Rroxscaffold_3G00257890

Actin-depolymerizing factor 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0016486
Physical Location & Seq
Forward (+)
52196362 .. 52197388
1027 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00257890.1

Sequence Viewer

Length: 438 bp
ATGATTAAGCTTTCTGATGTGGTACAGGCCAATGCAGCTTCTGGAATGGCTGTGCACGATGACTGCAAGCTGAGGTTCTTGGAGTTAAAGGCAAAGAGGAGCTATCGTTTCATTTTGTTCAAGATTGAGCAACAGCAAATTGTGGTTGATACGCTCGGGGAACCCAATGCAAGTTACGACGATTTCACCTCCAGTTTTCCTGCTGATGAGTGTCGTTATGCTGTTTATGATTTCGATTTCACTACCGAAGAGAACTGCCAGAAAAGCAAGATTTTCTTCATCGCATGGTCACCCGATACATCAAAGGTGAGGATGAAGATGGTGTATGCAAGTTCCAAGGACAGATTCAAAAGAGAATTGGACGGCATTTCATTTGAATTGCAAGCAACAGATCCAAGTGAGATGAGCTTGGACATAGTGAAAGGGCGAGCCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

145

Amino Acids

16.63

Weight (kDa)

5.23

Isoelectric Point (pI)

45.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 15 - 143 6.5e-41 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016486)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g02051 FvH4_6g08480
malus_domestica MD06G1079800.v1.1 MD14G1101700.v1.1
pyrus_communis pycom06g07660 pycom14g09820
rosa_chinensis RchiOBHm_Chr7g0198251
rosa_laevigata RLG00000003880
rosa_roxburghii Rroxscaffold_3G00257890
rosa_rugosa Rorug07G0046200
rosa_samantha Rh7AG169800 Rh7BG173000 Rh7CG179500
rosa_wichuraiana Rw7G014850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 386
AfaI GTAC 1 cut(s) 24
AgsI TTSAA 3 cut(s) 121, 349, 377
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 5 cut(s) 10, 38, 70, 102, 408
AluI AGCT 5 cut(s) 10, 38, 70, 102, 408
Alw21I GWGCWC 1 cut(s) 57
Alw44I GTGCAC 1 cut(s) 53
AlwI GGATC 1 cut(s) 386
AlwNI CAGNNNCTG 1 cut(s) 41
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 1 cut(s) 27
ApaLI GTGCAC 1 cut(s) 53
ApeKI GCWGC 1 cut(s) 35
AsuHPI GGTGA 3 cut(s) 178, 282, 319
AvaI CYCGRG 1 cut(s) 155
BaeGI GKGCMC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 57
BbvCI CCTCAGC 1 cut(s) 71
BbvI GCAGC 1 cut(s) 47
BccI CCATC 1 cut(s) 313
BceAI ACGGC 1 cut(s) 379
BisI GCNGC 1 cut(s) 36
BlsI GCNGC 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 155
BmiI GGNNCC 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 175
Bpu10I CCTNAGC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 336
BsaXI ACNNNNNCTCC 2 cut(s) 91, 121
Bse1I ACTGG 1 cut(s) 192
BseDI CCNNGG 1 cut(s) 336
BseGI GGATG 1 cut(s) 318
BseMII CTCAG 1 cut(s) 62
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 112
BseSI GKGCMC 1 cut(s) 57
BseXI GCAGC 1 cut(s) 47
BshFI GGCC 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 57
BsiHKCI CYCGRG 1 cut(s) 155
BsnI GGCC 1 cut(s) 29
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 1 cut(s) 391
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 162
BspPI GGATC 1 cut(s) 386
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 1 cut(s) 391
BssT1I CCWWGG 1 cut(s) 336
Bst6I CTCTTC 1 cut(s) 243
BstC8I GCNNGC 4 cut(s) 68, 384, 429, 433
BstDEI CTNAG 1 cut(s) 71
BstEII GGTNACC 1 cut(s) 288
BstF5I GGATG 1 cut(s) 318
BstKTI GATC 1 cut(s) 394
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 2 cut(s) 35, 264
BstPI GGTNACC 1 cut(s) 288
BstSLI GKGCMC 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 47
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BsuRI GGCC 1 cut(s) 29
BtgZI GCGATG 1 cut(s) 265
BtsCI GGATG 1 cut(s) 318
Cac8I GCNNGC 4 cut(s) 68, 384, 429, 433
CaiI CAGNNNCTG 1 cut(s) 41
Csp6I GTAC 1 cut(s) 23
CviAII CATG 1 cut(s) 285
CviJI RGCY 8 cut(s) 10, 29, 38, 50, 70, 102, 408, 431
CviKI_1 RGCY 8 cut(s) 10, 29, 38, 50, 70, 102, 408, 431
CviQI GTAC 1 cut(s) 23
DdeI CTNAG 1 cut(s) 71
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 336
Eco88I CYCGRG 1 cut(s) 155
Eco91I GGTNACC 1 cut(s) 288
EcoO65I GGTNACC 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 336
FaeI CATG 1 cut(s) 288
FaiI YATR 5 cut(s) 219, 228, 286, 327, 416
FalI AAGNNNNNCTT 2 cut(s) 260, 292
FatI CATG 1 cut(s) 284
Fnu4HI GCNGC 1 cut(s) 36
FokI GGATG 1 cut(s) 325
Fsp4HI GCNGC 1 cut(s) 36
GluI GCNGC 1 cut(s) 36
GsuI CTGGAG 1 cut(s) 175
HaeIII GGCC 1 cut(s) 29
Hin1II CATG 1 cut(s) 288
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 1 cut(s) 345
HphI GGTGA 3 cut(s) 178, 282, 319
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 1 cut(s) 16
Hpy188III TCNNGA 2 cut(s) 42, 121
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 1 cut(s) 182
HpyCH4V TGCA 6 cut(s) 35, 55, 66, 170, 329, 382
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 264
HpyF3I CTNAG 1 cut(s) 71
Hsp92II CATG 1 cut(s) 288
Kzo9I GATC 1 cut(s) 391
LmnI GCTCC 1 cut(s) 99
LpnPI CCDG 5 cut(s) 11, 27, 205, 213, 272
Lsp1109I GCAGC 1 cut(s) 47
MaeIII GTNAC 2 cut(s) 173, 288
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 3 cut(s) 260, 268, 328
MflI RGATCY 1 cut(s) 391
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 3 cut(s) 138, 356, 377
MnlI CCTC 4 cut(s) 66, 90, 199, 303
MseI TTAA 2 cut(s) 6, 86
MwoI GCNNNNNNNGC 2 cut(s) 35, 264
NdeII GATC 1 cut(s) 391
NlaIII CATG 1 cut(s) 288
NlaIV GGNNCC 1 cut(s) 162
NmuCI GTSAC 1 cut(s) 288
PfeI GAWTC 1 cut(s) 345
PkrI GCNGC 1 cut(s) 37
PspEI GGTNACC 1 cut(s) 288
PspN4I GGNNCC 1 cut(s) 162
PstNI CAGNNNCTG 1 cut(s) 41
PsuI RGATCY 1 cut(s) 391
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 6, 86
SatI GCNGC 1 cut(s) 36
Sau3AI GATC 1 cut(s) 391
SduI GDGCHC 1 cut(s) 57
SetI ASST 8 cut(s) 12, 40, 72, 77, 104, 191, 309, 410
Sse9I AATT 3 cut(s) 138, 356, 377
StyI CCWWGG 1 cut(s) 336
TaqI TCGA 1 cut(s) 234
TasI AATT 3 cut(s) 138, 356, 377
TfiI GAWTC 1 cut(s) 345
Tru1I TTAA 2 cut(s) 6, 86
Tru9I TTAA 2 cut(s) 6, 86
TseFI GTSAC 1 cut(s) 288
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 1 cut(s) 288
TspDTI ATGAA 4 cut(s) 100, 268, 329, 360
VneI GTGCAC 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.