pycom14g09820

Actin-depolymerizing factor 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0016486
Physical Location & Seq
Forward (+)
12069014 .. 12070141
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g09820.1

Sequence Viewer

Length: 393 bp
ATGGCTGTGCACGATGACTGCAAGTTGAGGTTCTTGGAGCTCAAAGCAAAAAGGAGCTATCGCTTTATTCTGTACAAGATTGAGCAACAACAAATTGTGATTGATAAGCTCGGGGAACCTAATGAGAGTTATGAGGATTTCACCGCCAGTTTTCCTGCTGATGAGTGTCGCTATGCTGTCTATGACTTCGATTTCACTACTGAAGAGAACTGCCAGAAAAGCAAGATTTTCTTCATTGCATGGTCACCGGATACATCAAAGGTGAGGATGAAGATGGTGTATGCTAGTTCCAAGGACAGATTCAAGAGAGAATTGGACGGCATTTCATTTGAATTGCAAGCAACAGATCCAAGTGAGATGAGCTTGGACATAGTGAAAGGGCGAGCCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

131

Amino Acids

15.26

Weight (kDa)

5.26

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 2 - 128 1.7e-40 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016486)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g02051 FvH4_6g08480
malus_domestica MD06G1079800.v1.1 MD14G1101700.v1.1
pyrus_communis pycom06g07660 pycom14g09820
rosa_chinensis RchiOBHm_Chr7g0198251
rosa_laevigata RLG00000003880
rosa_roxburghii Rroxscaffold_3G00257890
rosa_rugosa Rorug07G0046200
rosa_samantha Rh7AG169800 Rh7BG173000 Rh7CG179500
rosa_wichuraiana Rw7G014850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 144
AclWI GGATC 1 cut(s) 341
AcuI CTGAAG 1 cut(s) 222
AfaI GTAC 1 cut(s) 74
AgsI TTSAA 2 cut(s) 304, 332
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 4 cut(s) 40, 57, 109, 363
AluI AGCT 4 cut(s) 40, 57, 109, 363
Alw21I GWGCWC 2 cut(s) 12, 42
Alw44I GTGCAC 1 cut(s) 8
AlwI GGATC 1 cut(s) 341
Ama87I CYCGRG 1 cut(s) 110
ApaLI GTGCAC 1 cut(s) 8
AsuHPI GGTGA 3 cut(s) 133, 237, 274
AvaI CYCGRG 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 12
BanII GRGCYC 1 cut(s) 42
Bbv12I GWGCWC 2 cut(s) 12, 42
BccI CCATC 1 cut(s) 268
BceAI ACGGC 1 cut(s) 334
BciVI GTATCC 1 cut(s) 244
BfaI CTAG 1 cut(s) 285
BfuI GTATCC 1 cut(s) 244
BmeT110I CYCGRG 1 cut(s) 110
BmiI GGNNCC 1 cut(s) 117
BsaJI CCNNGG 1 cut(s) 291
BsaWI WCCGGW 1 cut(s) 247
Bse1I ACTGG 1 cut(s) 147
Bse3DI GCAATG 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 1 cut(s) 273
BseMI GCAATG 1 cut(s) 234
BseNI ACTGG 1 cut(s) 147
BseSI GKGCMC 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 12, 42
BsiHKCI CYCGRG 1 cut(s) 110
BsiSI CCGG 1 cut(s) 248
BsoBI CYCGRG 1 cut(s) 110
Bsp1286I GDGCHC 2 cut(s) 12, 42
Bsp1407I TGTACA 1 cut(s) 72
Bsp143I GATC 1 cut(s) 346
BspACI CCGC 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 117
BspPI GGATC 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 234
BsrGI TGTACA 1 cut(s) 72
BsrI ACTGG 1 cut(s) 147
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 1 cut(s) 346
BssT1I CCWWGG 1 cut(s) 291
Bst6I CTCTTC 1 cut(s) 198
BstAUI TGTACA 1 cut(s) 72
BstC8I GCNNGC 3 cut(s) 339, 384, 388
BstEII GGTNACC 1 cut(s) 243
BstF5I GGATG 1 cut(s) 273
BstKTI GATC 1 cut(s) 349
BstMBI GATC 1 cut(s) 346
BstMWI GCNNNNNNNGC 1 cut(s) 219
BstPI GGTNACC 1 cut(s) 243
BstSLI GKGCMC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
BsuI GTATCC 1 cut(s) 244
BtsCI GGATG 1 cut(s) 273
Cac8I GCNNGC 3 cut(s) 339, 384, 388
Csp6I GTAC 1 cut(s) 73
CviAII CATG 1 cut(s) 240
CviJI RGCY 6 cut(s) 5, 40, 57, 109, 363, 386
CviKI_1 RGCY 6 cut(s) 5, 40, 57, 109, 363, 386
CviQI GTAC 1 cut(s) 73
DpnI GATC 1 cut(s) 348
DpnII GATC 1 cut(s) 346
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 42
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 222
Eco88I CYCGRG 1 cut(s) 110
Eco91I GGTNACC 1 cut(s) 243
EcoICRI GAGCTC 1 cut(s) 40
EcoO65I GGTNACC 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 291
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 1 cut(s) 243
FaiI YATR 6 cut(s) 132, 174, 183, 241, 282, 371
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FatI CATG 1 cut(s) 239
FokI GGATG 1 cut(s) 280
FriOI GRGCYC 1 cut(s) 42
FspBI CTAG 1 cut(s) 285
HapII CCGG 1 cut(s) 248
Hin1II CATG 1 cut(s) 243
HinfI GANTC 1 cut(s) 300
HpaII CCGG 1 cut(s) 248
HphI GGTGA 3 cut(s) 133, 237, 274
Hpy166II GTNNAC 1 cut(s) 10
Hpy188III TCNNGA 1 cut(s) 304
Hpy8I GTNNAC 1 cut(s) 10
HpyCH4V TGCA 4 cut(s) 10, 21, 239, 337
HpyF10VI GCNNNNNNNGC 1 cut(s) 219
Hsp92II CATG 1 cut(s) 243
Kzo9I GATC 1 cut(s) 346
LmnI GCTCC 2 cut(s) 37, 54
LpnPI CCDG 4 cut(s) 160, 168, 227, 261
MaeI CTAG 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 243
MalI GATC 1 cut(s) 348
MboI GATC 1 cut(s) 346
MboII GAAGA 3 cut(s) 215, 223, 283
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 2 cut(s) 12, 42
MluCI AATT 3 cut(s) 93, 311, 332
MnlI CCTC 3 cut(s) 21, 127, 258
MspI CCGG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 219
NdeII GATC 1 cut(s) 346
NlaIII CATG 1 cut(s) 243
NlaIV GGNNCC 1 cut(s) 117
NmuCI GTSAC 1 cut(s) 243
PfeI GAWTC 1 cut(s) 300
Psp124BI GAGCTC 1 cut(s) 42
PspEI GGTNACC 1 cut(s) 243
PspN4I GGNNCC 1 cut(s) 117
PsuI RGATCY 1 cut(s) 346
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
SacI GAGCTC 1 cut(s) 42
Sau3AI GATC 1 cut(s) 346
SduI GDGCHC 2 cut(s) 12, 42
SetI ASST 7 cut(s) 32, 42, 59, 111, 121, 264, 365
Sse9I AATT 3 cut(s) 93, 311, 332
SsiI CCGC 1 cut(s) 144
SspMI CTAG 1 cut(s) 285
SstI GAGCTC 1 cut(s) 42
StyI CCWWGG 1 cut(s) 291
TaqI TCGA 1 cut(s) 189
TasI AATT 3 cut(s) 93, 311, 332
TatI WGTACW 1 cut(s) 72
TfiI GAWTC 1 cut(s) 300
TseFI GTSAC 1 cut(s) 243
Tsp45I GTSAC 1 cut(s) 243
TspDTI ATGAA 3 cut(s) 223, 284, 315
VneI GTGCAC 1 cut(s) 8
XspI CTAG 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.