MD14G1101700.v1.1

Actin-depolymerizing factor 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016486
Physical Location & Seq
Forward (+)
15670719 .. 15672446
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1101700.v1.1.491

Sequence Viewer

Length: 414 bp
ATGGCCAATGCAGCTTCTGGAATGGCTGTACACGATGACTGCAAGCTGAGGTTCTTGGAGCTCAAAGCAAAAAGGAGCTATCGCTTTATTCTGTACAAGATTGAGCAACAACAAATTGTGATTGATAAGCTCGGGGAACCTAATGAGAGTTATGAGGATTTCACCGCCAGTTTTCCTGCTGATGAGTGTCGCTATGCTGTCTATGACTTCGATTTCACTACTGAAGAAAACTGCCAGAAAAGCAAGATTTTCTTCATTGCTTGGTCACCGGATACATCAAAGGTGAGGATGAAGATGGTGTATGCTAGTTCCAAGGACAGATTCAAGAGAGAATTGGACGGCATTTCATTTGAATTGCAAGCAACAGATCCAAGTGAGATGAGCTTGGACATAGTGAAAGGGCGAGCCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

138

Amino Acids

15.86

Weight (kDa)

5.26

Isoelectric Point (pI)

51.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 7 - 135 7.3e-41 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016486)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g02051 FvH4_6g08480
malus_domestica MD06G1079800.v1.1 MD14G1101700.v1.1
pyrus_communis pycom06g07660 pycom14g09820
rosa_chinensis RchiOBHm_Chr7g0198251
rosa_laevigata RLG00000003880
rosa_roxburghii Rroxscaffold_3G00257890
rosa_rugosa Rorug07G0046200
rosa_samantha Rh7AG169800 Rh7BG173000 Rh7CG179500
rosa_wichuraiana Rw7G014850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 165
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 2 cut(s) 30, 95
AgsI TTSAA 2 cut(s) 325, 353
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
AluBI AGCT 6 cut(s) 14, 46, 61, 78, 130, 384
AluI AGCT 6 cut(s) 14, 46, 61, 78, 130, 384
Alw21I GWGCWC 1 cut(s) 63
AlwI GGATC 1 cut(s) 362
AlwNI CAGNNNCTG 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 11
AsuHPI GGTGA 3 cut(s) 154, 258, 295
AvaI CYCGRG 1 cut(s) 131
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 63
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 1 cut(s) 23
BccI CCATC 1 cut(s) 289
BceAI ACGGC 1 cut(s) 355
BciVI GTATCC 1 cut(s) 265
BfaI CTAG 1 cut(s) 306
BfuI GTATCC 1 cut(s) 265
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
BmeT110I CYCGRG 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 138
Bpu10I CCTNAGC 1 cut(s) 47
BsaJI CCNNGG 1 cut(s) 312
BsaWI WCCGGW 1 cut(s) 268
Bse1I ACTGG 1 cut(s) 168
Bse3DI GCAATG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 1 cut(s) 294
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 1 cut(s) 168
BseXI GCAGC 1 cut(s) 23
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 1 cut(s) 269
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 63
Bsp1407I TGTACA 2 cut(s) 28, 93
Bsp143I GATC 1 cut(s) 367
BspACI CCGC 1 cut(s) 165
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 138
BspPI GGATC 1 cut(s) 362
BsrDI GCAATG 1 cut(s) 255
BsrGI TGTACA 2 cut(s) 28, 93
BsrI ACTGG 1 cut(s) 168
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 1 cut(s) 367
BssT1I CCWWGG 1 cut(s) 312
BstAUI TGTACA 2 cut(s) 28, 93
BstC8I GCNNGC 4 cut(s) 44, 360, 405, 409
BstDEI CTNAG 1 cut(s) 47
BstEII GGTNACC 1 cut(s) 264
BstF5I GGATG 1 cut(s) 294
BstKTI GATC 1 cut(s) 370
BstMBI GATC 1 cut(s) 367
BstMWI GCNNNNNNNGC 2 cut(s) 11, 240
BstPI GGTNACC 1 cut(s) 264
BstV1I GCAGC 1 cut(s) 23
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BsuI GTATCC 1 cut(s) 265
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 294
Cac8I GCNNGC 4 cut(s) 44, 360, 405, 409
CaiI CAGNNNCTG 1 cut(s) 17
Csp6I GTAC 2 cut(s) 29, 94
CviJI RGCY 9 cut(s) 5, 14, 26, 46, 61, 78, 130, 384, 407
CviKI_1 RGCY 9 cut(s) 5, 14, 26, 46, 61, 78, 130, 384, 407
CviQI GTAC 2 cut(s) 29, 94
DdeI CTNAG 1 cut(s) 47
DpnI GATC 1 cut(s) 369
DpnII GATC 1 cut(s) 367
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 312
Eco24I GRGCYC 1 cut(s) 63
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 131
Eco91I GGTNACC 1 cut(s) 264
EcoICRI GAGCTC 1 cut(s) 61
EcoO65I GGTNACC 1 cut(s) 264
EcoT14I CCWWGG 1 cut(s) 312
EcoT38I GRGCYC 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 312
FaiI YATR 5 cut(s) 153, 195, 204, 303, 392
FalI AAGNNNNNCTT 2 cut(s) 236, 268
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 1 cut(s) 301
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 1 cut(s) 306
GluI GCNGC 1 cut(s) 12
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 269
HinfI GANTC 1 cut(s) 321
HpaII CCGG 1 cut(s) 269
HphI GGTGA 3 cut(s) 154, 258, 295
Hpy166II GTNNAC 1 cut(s) 31
Hpy188III TCNNGA 2 cut(s) 18, 325
Hpy8I GTNNAC 1 cut(s) 31
HpyCH4V TGCA 3 cut(s) 11, 42, 358
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 240
HpyF3I CTNAG 1 cut(s) 47
Kzo9I GATC 1 cut(s) 367
LmnI GCTCC 2 cut(s) 58, 75
LpnPI CCDG 5 cut(s) 3, 181, 189, 248, 282
Lsp1109I GCAGC 1 cut(s) 23
MaeI CTAG 1 cut(s) 306
MaeIII GTNAC 1 cut(s) 264
MalI GATC 1 cut(s) 369
MboI GATC 1 cut(s) 367
MboII GAAGA 3 cut(s) 236, 244, 304
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 1 cut(s) 63
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 114, 332, 353
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 3 cut(s) 42, 148, 279
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 269
MwoI GCNNNNNNNGC 2 cut(s) 11, 240
NdeII GATC 1 cut(s) 367
NlaIV GGNNCC 1 cut(s) 138
NmuCI GTSAC 1 cut(s) 264
PfeI GAWTC 1 cut(s) 321
PkrI GCNGC 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 63
PspEI GGTNACC 1 cut(s) 264
PspN4I GGNNCC 1 cut(s) 138
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 367
RsaI GTAC 2 cut(s) 30, 95
RsaNI GTAC 2 cut(s) 29, 94
SacI GAGCTC 1 cut(s) 63
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 1 cut(s) 367
SduI GDGCHC 1 cut(s) 63
SetI ASST 9 cut(s) 16, 48, 53, 63, 80, 132, 142, 285, 386
Sse9I AATT 3 cut(s) 114, 332, 353
SsiI CCGC 1 cut(s) 165
SspMI CTAG 1 cut(s) 306
SstI GAGCTC 1 cut(s) 63
StyI CCWWGG 1 cut(s) 312
TaqI TCGA 1 cut(s) 210
TasI AATT 3 cut(s) 114, 332, 353
TatI WGTACW 2 cut(s) 28, 93
TfiI GAWTC 1 cut(s) 321
TseFI GTSAC 1 cut(s) 264
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 264
TspDTI ATGAA 3 cut(s) 244, 305, 336
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.