MD06G1098100.v1.1

intracellular protein transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008611
Physical Location & Seq
Forward (+)
23348598 .. 23352397
3800 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1098100.v1.1.491

Sequence Viewer

Length: 2133 bp
ATGAAAAAGGAAGGAGAAAGTGGGGAAGGGGGAGAAGAAAAGGAGGAAAATTTGGGAGAAAAGTTGAGAGGAAGGAGGAGGAGAGGAGTATTGATTAGAAAAAGCACTGGTGGTGGCCCAACCACTCCAGTGTCCTCATGGAGTTGGAGACCTTGTTGCCCTTTTGGCCTTGATCCCATCATCAATAAAGACTCCCACTTTACCATTAATACCAGCTCCTCCTCCTCCTACTCTGTCTCTGCGAGAAAGCTAGCTGCTGCTCTTTGGGAGTTTCAACACTTCCAATTCCTTCCAAAGATGCACAGAGGTCCTCACACTAATGGCGGCGGCGGCGGTGCTCCACCTCCTAGGCTGAGACACCTTCAGCTCCAACACCACCACCTCAGCAGAGACAAGAAGGCTCTTGATCTTTCTCATTTTTTGGCTGATAATTCTCCCAGTTCTCCTGACCAGCCAGAAAGTGCAAGCAGTTTAAGAAGGCATATTGCAGCATCGTTGATGCAACATCATCGGACAATTGAAAGGAACAAATTGAACAATCACATTATGCAGCCTGCATCTCCTGCAAGCTACGGTAGTTCACTGGAGGTGGCTCCGTATAACCCTGCAGTCACCCCTAGCAGTTCTTTAGACTTTAAGGGAAGGGTTGGCGATTCACACTATAATCTTAAAACATCTACAGAACTGCTAAAAGTTTTGAACCGGATTTGGAGCTTGGAGGAACAACATACATCTAATATAGCATTGATAAAGGCGTTGAAAACAGAGTTAGATCATGCCCGTGTTAAGATCAAAGAGTTGCTACGGGTTCGGCAAGCTGATCGACATGAGCTAGATGATTTGGTGAAACAAATTTCAGAAGATAAACAAGTTAGAAAGAGCAAAGAACAGGATCGAATCCATGCTGCAGTTCAGTCCGTGAGGGATGAGCTAGAAGATGAGAGGAAGTTAAGAAAACGATCAGAAAGTCTGCACAGGAAGTTGGCGAGGGAGCTTGCTGAGGTGAAATCTTCTCTTGCTAATTCTGTGAAAGAGCTTGAAAGAGAGAGAAAATCGAGGAGGTTGTTAGAAGACCTGTGCGACGAGTTTGCTACGGGAATAAAAGAATATGAACAAGAGGTGCATTCTTTGAAACAGAAGACTGACAAGGATTGGACGGGAAGATCTGATCATGGTAGATTGATTCTCCATGTATCTGAATCGTGGCTGGATGAGCGGATGCAGATGCAGCTAGATGAAGCCGAGTGTGGCGTTTCAGGAAATAACTCAATAGCGGATAAGTTAAGCCTTGAAATAGAGGCTTTCCTTAGAGCTAAACATATGAATTCTCTAAAGCATACTGAAAATCTGATGACAAGAAATCGTAGGAATTCAATGGAATCTGTCCCTCTGAATGAGGTTGTGAGTGCGCCTCAAGATGCGGGTGAAGAAGAAGATGATTCTTTTGGCAGTGATTCAAATTGTTTTGAGCTCAATAAGCCTACCAACGGTGACTTCAAATTGGTTGGCGATGAAGCTGGTAAAAATCATATTGAGAAAAAGGTGAAATCAGATCCCTCAAAGAAAAAGCCGATCTCTTCTGAAAGGGTAAGAAGTCGGAGTCCCTCCAGCTTGCAAGTGAAGTTTGAAGAACAGATGGCTTGGGCCGTGTCTTGTAATGGAAATAAGAAGTCCCAGATGGAGAATCCAGAACAGGAGAAAATTGAAGGGAAGCCGCCTGAAATAAGCACATCCCAAAAATCTGAGCATTGTGAAGCCACTGAAGATAATGCTTACAGAAAGGGAAATAAACAAGATGAAACCCATGTTTCGAATTCGAATCACATGGCTGAAAACCTTATAAGAAGTCAACTACTGATGCCGGATGGTGGGGGGAATGTACATTCTGAAAACAATTACAATGAGGCTTCCTGCAGTAATACTGGCTGGAGGAATCAGGCAAGTCCAGTAAGACAGTGGATGGCGAGACTCGCATCTCCTGCTCGTGACATGTCCGAGTCTTCTTCAAAGTTGCCTCCACGGACAAGGGAAAACACCTTGAAGGCAAAACTTCTTGAAGCAAGGTCGAGGGGACAACGGACACGTCCAAAAGCTTCCAAAAACACGTCTAAATCCTCTGAAAGGGTAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0009507 GO:0009536 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

711

Amino Acids

80.14

Weight (kDa)

9.06

Isoelectric Point (pI)

59.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1841
AccBSI CCGCTC 1 cut(s) 1216
AciI CCGC 8 cut(s) 324, 327, 330, 333, 1216, 1274, 1421, 1715
AclWI GGATC 3 cut(s) 167, 900, 1547
AcsI RAATTY 5 cut(s) 49, 852, 1324, 1369, 1813
AcuI CTGAAG 2 cut(s) 347, 1782
AfaI GTAC 1 cut(s) 1881
AfiI CCNNNNNNNGG 3 cut(s) 1487, 1868, 2121
AflIII ACRYGT 3 cut(s) 1989, 2081, 2103
AjiI CACGTC 2 cut(s) 2084, 2106
AjuI GAANNNNNNNTTGG 2 cut(s) 1478, 1510
AleI CACNNNNGTG 1 cut(s) 128
Alw21I GWGCWC 2 cut(s) 340, 1473
Alw26I GTCTC 5 cut(s) 142, 241, 349, 384, 1960
AlwI GGATC 3 cut(s) 167, 900, 1547
AoxI GGCC 3 cut(s) 115, 166, 1644
ApeKI GCWGC 6 cut(s) 254, 257, 488, 550, 905, 1228
ApoI RAATTY 5 cut(s) 49, 852, 1324, 1369, 1813
AseI ATTAAT 1 cut(s) 207
AspA2I CCTAGG 1 cut(s) 347
AspLEI GCGC 1 cut(s) 1411
AspS9I GGNCC 3 cut(s) 116, 308, 1644
AsuHPI GGTGA 6 cut(s) 604, 856, 1015, 1436, 1502, 1555
AsuII TTCGAA 2 cut(s) 1811, 1817
AsuNHI GCTAGC 1 cut(s) 250
AvaII GGWCC 1 cut(s) 308
AvrII CCTAGG 1 cut(s) 347
BanII GRGCYC 1 cut(s) 1473
BauI CACGAG 1 cut(s) 1983
BbsI GAAGAC 3 cut(s) 1077, 1145, 1992
Bbv12I GWGCWC 2 cut(s) 340, 1473
BbvCI CCTCAGC 2 cut(s) 383, 999
BbvI GCAGC 6 cut(s) 241, 244, 500, 562, 892, 1240
BccI CCATC 5 cut(s) 185, 1630, 1672, 1859, 1954
BceAI ACGGC 1 cut(s) 1631
BcgI CGANNNNNNTGC 6 cut(s) 491, 525, 803, 837, 1070, 1104
BclI TGATCA 1 cut(s) 1168
BcoDI GTCTC 5 cut(s) 142, 241, 349, 384, 1960
BfaI CTAG 6 cut(s) 251, 348, 618, 833, 932, 1232
BfmI CTRYAG 4 cut(s) 606, 678, 906, 1912
BglI GCCNNNNNGGC 1 cut(s) 165
BglII AGATCT 1 cut(s) 1163
BlnI CCTAGG 1 cut(s) 347
Bme18I GGWCC 1 cut(s) 308
BmgBI CACGTC 2 cut(s) 2084, 2106
BmgT120I GGNCC 3 cut(s) 116, 308, 1644
BmiI GGNNCC 1 cut(s) 594
BmrI ACTGGG 1 cut(s) 432
BmsI GCATC 9 cut(s) 288, 489, 500, 566, 1209, 1215, 1408, 1848, 1982
BmtI GCTAGC 1 cut(s) 254
BmuI ACTGGG 1 cut(s) 432
BpiI GAAGAC 3 cut(s) 1077, 1145, 1992
BplI GAGNNNNNCTC 2 cut(s) 1396, 1428
BpmI CTGGAG 4 cut(s) 111, 605, 1591, 1948
Bpu10I CCTNAGC 2 cut(s) 383, 999
Bpu14I TTCGAA 2 cut(s) 1811, 1817
BpuEI CTTGAG 1 cut(s) 1398
BsaI GGTCTC 1 cut(s) 142
BsaJI CCNNGG 2 cut(s) 347, 2018
BsaWI WCCGGW 1 cut(s) 702
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bsc4I CCNNNNNNNGG 3 cut(s) 1487, 1868, 2121
Bse1I ACTGG 6 cut(s) 112, 128, 438, 588, 1927, 1946
BseDI CCNNGG 2 cut(s) 347, 2018
BseGI GGATG 6 cut(s) 931, 1216, 1224, 1730, 1870, 1965
BseLI CCNNNNNNNGG 3 cut(s) 1487, 1868, 2121
BseMII CTCAG 4 cut(s) 344, 397, 990, 1734
BseNI ACTGG 6 cut(s) 112, 128, 438, 588, 1927, 1946
BseRI GAGGAG 7 cut(s) 91, 94, 99, 208, 211, 214, 1072
BseXI GCAGC 6 cut(s) 241, 244, 500, 562, 892, 1240
BsgI GTGCAG 1 cut(s) 956
BshFI GGCC 3 cut(s) 117, 168, 1646
BsiHKAI GWGCWC 2 cut(s) 340, 1473
BsiSI CCGG 2 cut(s) 703, 1862
BslFI GGGAC 4 cut(s) 1370, 1587, 1657, 2085
BslI CCNNNNNNNGG 3 cut(s) 1487, 1868, 2121
BsmAI GTCTC 5 cut(s) 142, 241, 349, 384, 1960
BsmFI GGGAC 4 cut(s) 1370, 1587, 1657, 2085
BsmI GAATGC 1 cut(s) 1123
BsnI GGCC 3 cut(s) 117, 168, 1646
Bso31I GGTCTC 1 cut(s) 142
Bsp119I TTCGAA 2 cut(s) 1811, 1817
Bsp1286I GDGCHC 2 cut(s) 340, 1473
Bsp1407I TGTACA 1 cut(s) 1879
BspACI CCGC 8 cut(s) 324, 327, 330, 333, 1216, 1274, 1421, 1715
BspANI GGCC 3 cut(s) 117, 168, 1646
BspCNI CTCAG 4 cut(s) 345, 396, 991, 1735
BspLI GGNNCC 1 cut(s) 594
BspMAI CTGCAG 3 cut(s) 610, 910, 1916
BspOI GCTAGC 1 cut(s) 254
BspPI GGATC 3 cut(s) 167, 900, 1547
BspT104I TTCGAA 2 cut(s) 1811, 1817
BspTNI GGTCTC 1 cut(s) 142
BsrBI CCGCTC 1 cut(s) 1216
BsrGI TGTACA 1 cut(s) 1879
BsrI ACTGG 6 cut(s) 112, 128, 438, 588, 1927, 1946
BssECI CCNNGG 2 cut(s) 347, 2018
BssSI CACGAG 1 cut(s) 1983
BssT1I CCWWGG 1 cut(s) 347
Bst2BI CACGAG 1 cut(s) 1983
Bst4CI ACNGT 3 cut(s) 575, 1490, 1956
Bst6I CTCTTC 1 cut(s) 1582
BstAPI GCANNNNNTGC 2 cut(s) 563, 1979
BstAUI TGTACA 1 cut(s) 1879
BstBI TTCGAA 2 cut(s) 1811, 1817
BstC8I GCNNGC 7 cut(s) 252, 466, 555, 568, 816, 996, 1613
BstDEI CTNAG 5 cut(s) 353, 383, 999, 1307, 1743
BstDSI CCRYGG 1 cut(s) 2018
BstENI CCTNNNNNAGG 1 cut(s) 2119
BstF5I GGATG 6 cut(s) 931, 1216, 1224, 1730, 1870, 1965
BstHHI GCGC 1 cut(s) 1411
BstMAI GTCTC 5 cut(s) 142, 241, 349, 384, 1960
BstMWI GCNNNNNNNGC 8 cut(s) 165, 330, 563, 1213, 1228, 1477, 1970, 1979
BstNSI RCATGY 1 cut(s) 1993
BstSFI CTRYAG 4 cut(s) 606, 678, 906, 1912
BstV1I GCAGC 6 cut(s) 241, 244, 500, 562, 892, 1240
BstV2I GAAGAC 3 cut(s) 1077, 1145, 1992
BstX2I RGATCY 2 cut(s) 1163, 1552
BstYI RGATCY 2 cut(s) 1163, 1552
BsuRI GGCC 3 cut(s) 117, 168, 1646
BtgI CCRYGG 1 cut(s) 2018
BtgZI GCGATG 1 cut(s) 1524
BtrI CACGTC 2 cut(s) 2084, 2106
BtsCI GGATG 6 cut(s) 931, 1216, 1224, 1730, 1870, 1965
BtsI GCAGTG 1 cut(s) 1456
BtsIMutI CAGTG 6 cut(s) 105, 135, 581, 1456, 1758, 1961
Cac8I GCNNGC 7 cut(s) 252, 466, 555, 568, 816, 996, 1613
CfoI GCGC 1 cut(s) 1411
Cfr13I GGNCC 3 cut(s) 116, 308, 1644
Csp6I GTAC 1 cut(s) 1880
CviAII CATG 9 cut(s) 138, 776, 827, 902, 1172, 1190, 1805, 1825, 1990
CviQI GTAC 1 cut(s) 1880
DdeI CTNAG 5 cut(s) 353, 383, 999, 1307, 1743
Eam1104I CTCTTC 1 cut(s) 1582
EarI CTCTTC 1 cut(s) 1582
Ecl136II GAGCTC 1 cut(s) 1471
Eco130I CCWWGG 1 cut(s) 347
Eco24I GRGCYC 1 cut(s) 1473
Eco31I GGTCTC 1 cut(s) 142
Eco47I GGWCC 1 cut(s) 308
Eco53kI GAGCTC 1 cut(s) 1471
Eco57I CTGAAG 2 cut(s) 347, 1782
EcoICRI GAGCTC 1 cut(s) 1471
EcoNI CCTNNNNNAGG 1 cut(s) 2119
EcoO109I RGGNCCY 1 cut(s) 308
EcoRI GAATTC 3 cut(s) 1324, 1369, 1813
EcoT14I CCWWGG 1 cut(s) 347
EcoT38I GRGCYC 1 cut(s) 1473
ErhI CCWWGG 1 cut(s) 347
FaeI CATG 9 cut(s) 141, 779, 830, 905, 1175, 1193, 1808, 1828, 1993
FaqI GGGAC 4 cut(s) 1370, 1587, 1657, 2085
FatI CATG 9 cut(s) 137, 775, 826, 901, 1171, 1189, 1804, 1824, 1989
FauI CCCGC 1 cut(s) 1414
FauNDI CATATG 1 cut(s) 1320
FbaI TGATCA 1 cut(s) 1168
FokI GGATG 6 cut(s) 938, 1223, 1231, 1717, 1877, 1972
FriOI GRGCYC 1 cut(s) 1473
FspBI CTAG 6 cut(s) 251, 348, 618, 833, 932, 1232
GlaI GCGC 1 cut(s) 1410
GsuI CTGGAG 4 cut(s) 111, 605, 1591, 1948
HaeIII GGCC 3 cut(s) 117, 168, 1646
HapII CCGG 2 cut(s) 703, 1862
HhaI GCGC 1 cut(s) 1411
Hin1II CATG 9 cut(s) 141, 779, 830, 905, 1175, 1193, 1808, 1828, 1993
Hin6I GCGC 1 cut(s) 1409
HinP1I GCGC 1 cut(s) 1409
HincII GTYRAC 1 cut(s) 1850
HindII GTYRAC 1 cut(s) 1850
HindIII AAGCTT 1 cut(s) 2091
HpaII CCGG 2 cut(s) 703, 1862
HphI GGTGA 6 cut(s) 604, 856, 1015, 1436, 1502, 1555
Hpy166II GTNNAC 3 cut(s) 581, 1850, 2128
Hpy188III TCNNGA 7 cut(s) 404, 446, 1257, 1415, 1688, 1985, 2054
Hpy8I GTNNAC 3 cut(s) 581, 1850, 2128
Hpy99I CGWCG 1 cut(s) 1085
HpyCH4III ACNGT 3 cut(s) 575, 1490, 1956
HpyCH4IV ACGT 2 cut(s) 2083, 2105
HpyF10VI GCNNNNNNNGC 8 cut(s) 165, 330, 563, 1213, 1228, 1477, 1970, 1979
HpyF3I CTNAG 5 cut(s) 353, 383, 999, 1307, 1743
HpySE526I ACGT 2 cut(s) 2083, 2105
Hsp92II CATG 9 cut(s) 141, 779, 830, 905, 1175, 1193, 1808, 1828, 1993
HspAI GCGC 1 cut(s) 1409
Ksp22I TGATCA 1 cut(s) 1168
LmnI GCTCC 6 cut(s) 221, 343, 372, 598, 711, 991
Lsp1109I GCAGC 6 cut(s) 241, 244, 500, 562, 892, 1240
LweI GCATC 9 cut(s) 288, 489, 500, 566, 1209, 1215, 1408, 1848, 1982
MaeI CTAG 6 cut(s) 251, 348, 618, 833, 932, 1232
MaeII ACGT 2 cut(s) 2083, 2105
MaeIII GTNAC 3 cut(s) 610, 1490, 1985
MbiI CCGCTC 1 cut(s) 1216
MfeI CAATTG 1 cut(s) 516
MflI RGATCY 2 cut(s) 1163, 1552
MhlI GDGCHC 2 cut(s) 340, 1473
MlyI GAGTC 4 cut(s) 185, 1609, 1962, 2006
MmeI TCCRAC 3 cut(s) 125, 394, 1577
MseI TTAA 7 cut(s) 207, 473, 636, 669, 786, 950, 1283
MslI CAYNNNNRTG 3 cut(s) 128, 318, 780
MspI CCGG 2 cut(s) 703, 1862
MunI CAATTG 1 cut(s) 516
Mva1269I GAATGC 1 cut(s) 1123
MwoI GCNNNNNNNGC 8 cut(s) 165, 330, 563, 1213, 1228, 1477, 1970, 1979
NdeI CATATG 1 cut(s) 1320
NheI GCTAGC 1 cut(s) 250
NlaIII CATG 9 cut(s) 141, 779, 830, 905, 1175, 1193, 1808, 1828, 1993
NlaIV GGNNCC 1 cut(s) 594
NmeAIII GCCGAG 1 cut(s) 1267
NmuCI GTSAC 3 cut(s) 610, 1490, 1985
NspI RCATGY 1 cut(s) 1993
NspV TTCGAA 2 cut(s) 1811, 1817
OliI CACNNNNGTG 1 cut(s) 128
PciI ACATGT 1 cut(s) 1989
PctI GAATGC 1 cut(s) 1123
PleI GAGTC 4 cut(s) 185, 1608, 1962, 2005
PpsI GAGTC 4 cut(s) 185, 1608, 1962, 2005
PpuMI RGGWCCY 1 cut(s) 308
PscI ACATGT 1 cut(s) 1989
PshBI ATTAAT 1 cut(s) 207
PsiI TTATAA 1 cut(s) 1841
Psp124BI GAGCTC 1 cut(s) 1473
Psp5II RGGWCCY 1 cut(s) 308
PspN4I GGNNCC 1 cut(s) 594
PspPI GGNCC 3 cut(s) 116, 308, 1644
PspPPI RGGWCCY 1 cut(s) 308
PstI CTGCAG 3 cut(s) 610, 910, 1916
PsuI RGATCY 2 cut(s) 1163, 1552
RsaI GTAC 1 cut(s) 1881
RsaNI GTAC 1 cut(s) 1880
RseI CAYNNNNRTG 3 cut(s) 128, 318, 780
SacI GAGCTC 1 cut(s) 1473
SaqAI TTAA 7 cut(s) 207, 473, 636, 669, 786, 950, 1283
Sau96I GGNCC 3 cut(s) 116, 308, 1644
SchI GAGTC 4 cut(s) 185, 1609, 1962, 2006
SduI GDGCHC 2 cut(s) 340, 1473
SfaNI GCATC 9 cut(s) 288, 489, 500, 566, 1209, 1215, 1408, 1848, 1982
SfcI CTRYAG 4 cut(s) 606, 678, 906, 1912
SfuI TTCGAA 2 cut(s) 1811, 1817
SinI GGWCC 1 cut(s) 308
SmiMI CAYNNNNRTG 3 cut(s) 128, 318, 780
SmlI CTYRAG 1 cut(s) 1413
SmoI CTYRAG 1 cut(s) 1413
SsiI CCGC 8 cut(s) 324, 327, 330, 333, 1216, 1274, 1421, 1715
SspMI CTAG 6 cut(s) 251, 348, 618, 833, 932, 1232
SstI GAGCTC 1 cut(s) 1473
StyI CCWWGG 1 cut(s) 347
TaaI ACNGT 3 cut(s) 575, 1490, 1956
TaiI ACGT 2 cut(s) 2086, 2108
TaqI TCGA 6 cut(s) 823, 895, 1055, 1811, 1817, 2066
TatI WGTACW 1 cut(s) 1879
TauI GCSGC 4 cut(s) 327, 330, 333, 1717
Tru1I TTAA 7 cut(s) 207, 473, 636, 669, 786, 950, 1283
Tru9I TTAA 7 cut(s) 207, 473, 636, 669, 786, 950, 1283
TscAI CASTG 6 cut(s) 112, 135, 588, 1456, 1765, 1961
TseFI GTSAC 3 cut(s) 610, 1490, 1985
TseI GCWGC 6 cut(s) 254, 257, 488, 550, 905, 1228
Tsp45I GTSAC 3 cut(s) 610, 1490, 1985
TspDTI ATGAA 6 cut(s) 17, 1125, 1251, 1337, 1527, 1812
TspGWI ACGGA 4 cut(s) 585, 907, 2035, 2092
TspRI CASTG 6 cut(s) 112, 135, 588, 1456, 1765, 1961
VpaK11BI GGWCC 1 cut(s) 308
VspI ATTAAT 1 cut(s) 207
XagI CCTNNNNNAGG 1 cut(s) 2119
XapI RAATTY 5 cut(s) 49, 852, 1324, 1369, 1813
XceI RCATGY 1 cut(s) 1993
XcmI CCANNNNNNNNNTGG 2 cut(s) 135, 1953
XmaJI CCTAGG 1 cut(s) 347
XspI CTAG 6 cut(s) 251, 348, 618, 833, 932, 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.