Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0015626

Physical Location & Seq

Genomic Coordinates Forward (+)

34812277 .. 34813997

Gene Structure 1721 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1215800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGATCACGGATAAGAAAGCTGCAAATGCAATGGGAGGCAACACATCTCGAGCTTGCGATAGCTGTTTGGTGAAACGGGCACGATGGTTTTGTGCTGCTGATGATGCTTTTCTTTGCCAAAGATGTGATGCCTCGGTGCACTCGGCGAACCAGTTAGCAAGCAGACACGAGAGGGTTCGGCTCCAAGGTTCATCTTTCAAGGCCAATGCATCGGTGCAGCTGATTGCGGACCAAGCTGCACCGGCTTGGCACAAGGGGTTCAAGCGCAAGGCGCGAACCCCTCGCCACAACAACAAGCCTGGATTCGTGGCCAAAGATGAGCAAGAGAAAGTAGGGCTACACCCTCCTCTACCTTTTGTTCCGGAAATCGGGTGCCAAGAAGCAAATTTGATGGAGGATGAGGAAAATGAAGATCTAGAGCAGCTTCTTTATAGGGTTCCAATTTATGACCCTTTTGCAGCCGAGCTTTGCAATATTTCGTCAAGTGATCAGGTTGGGAACAATACTGGTGCTATTGATCTCGATGCCGAAGAGATTGAGATCAGAAATACGGGAAATGACTTGGACAATTTGGGAGGGTTTCTACGGTCGGATATGGAGCTGGCGGAGTTTGCTGCTGATGTGGAGAACCTGCTTGGAGGGGGACTAGATGAGGATTGTAGTGACATCAAAGGAGTCCTGGGGCTATTGGACGAGGGTAAAGAAGTGAAGGTTGAAGAGGAAGTTGAAGGGATAGAATTTAATCCTTCGATCTTGGATTGGAGCTTGTATTACGATCAGTCATCAGCTGTACCGGTACTAATGGGAAGTGAGGAGGACAGGGAGGAAGAGGAGAAGGTAGTCCTGCTCATGGGGGCTCAGAGTAGTGATGAGATTGGATTCATGGAGAACAAGCAAATAGTGATGAAGAGGAAGAACATATGTTTGAAGCTAGATTATGAGGCAGTCATCACTGCCTGGGCAAGCCAAGGCTCTCAATGGACGACTGGAACTCGACCGGAGCTCAACGCTGACGAAGGCTGGCTAGACTGCATGGGTATGTGGCCTACAGAAATTCATCAACCACATGGAGATTCAGCAGGATGTGTAAGTCGAGGAAAAGGCGATGACCCGGGAAGAGAAGCAAGAGTTTCTAGGTACAGAGAGAAGCGAAGGACGCGACTGTTTTCGAAGAAGATAAGATACGAAGTTAGGAAGCTGAACGCGGAGAAGCGGCCTAGAATGAAAGGTCGATTCGTTAAGAGGACAACGTCCAGCTTTGGTGGGGTGGGAAGTAGTAGTAGTAATGCTCCTGCGGCTGCATTTCCCTACCTCATGATCAATAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001763 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006355 GO:0007275 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009641 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0010016 GO:0010223 GO:0010346 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045893 GO:0045935 GO:0048367 GO:0048518 GO:0048522 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0070013 GO:0080090 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:1905393 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CCT zf-B_box

Protein Analysis

443

Amino Acids

49.11

Weight (kDa)

5.16

Isoelectric Point (pI)

54.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-B_box	PF00643	16 - 59	1.5e-06	B-box zinc finger
CCT	PF06203	373 - 415	4.5e-17	CCT motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015626)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g12150
malus_domestica	MD06G1215800.v1.1 MD14G1226400.v1.1
prunus_persica	Prupe.5G221000_v2.0.a1
pyrus_communis	pycom06g19250 pycom14g18780
rosa_chinensis	RchiOBHm_Chr7g0185411
rosa_laevigata	RLG00000004915
rosa_multiflora	Rmu_sc0001761.1_g000007
rosa_roxburghii	Rroxscaffold_3G00268960
rosa_rugosa	Rorug06G0468900
rosa_samantha	Rh7AG073900 Rh7BG073800 Rh7CG073900 Rh7DG073900
rosa_wichuraiana	Rw7G006140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	639
AccB1I	GGYRCC	1 cut(s)	372
AccII	CGCG	3 cut(s)	274, 1159, 1205
AccIII	TCCGGA	1 cut(s)	361
AciI	CCGC	5 cut(s)	227, 605, 1205, 1213, 1295
AcoI	YGGCCR	1 cut(s)	309
AcsI	RAATTY	3 cut(s)	385, 737, 1053
AfaI	GTAC	3 cut(s)	792, 798, 1139
AfiI	CCNNNNNNNGG	2 cut(s)	368, 850
AgeI	ACCGGT	1 cut(s)	793
AgsI	TTSAA	5 cut(s)	199, 262, 716, 728, 928
AjnI	CCWGG	3 cut(s)	298, 678, 956
Alw21I	GWGCWC	2 cut(s)	141, 1005
Alw44I	GTGCAC	1 cut(s)	137
Ama87I	CYCGRG	2 cut(s)	48, 1111
Aor13HI	TCCGGA	1 cut(s)	361
AoxI	GGCC	4 cut(s)	201, 309, 1043, 1214
ApaLI	GTGCAC	1 cut(s)	137
ApeKI	GCWGC	8 cut(s)	20, 95, 217, 236, 421, 458, 614, 1298
ApoI	RAATTY	3 cut(s)	385, 737, 1053
AsiGI	ACCGGT	1 cut(s)	793
AspLEI	GCGC	2 cut(s)	267, 274
AspS9I	GGNCC	1 cut(s)	229
AsuC2I	CCSGG	2 cut(s)	1112, 1113
AsuHPI	GGTGA	1 cut(s)	82
AsuII	TTCGAA	1 cut(s)	1169
AvaI	CYCGRG	2 cut(s)	48, 1111
AvaII	GGWCC	1 cut(s)	229
BaeGI	GKGCMC	2 cut(s)	82, 141
BalI	TGGCCA	1 cut(s)	311
BanI	GGYRCC	1 cut(s)	372
BanII	GRGCYC	2 cut(s)	859, 1005
BarI	GAAGNNNNNNTAC	2 cut(s)	1166, 1198
BauI	CACGAG	1 cut(s)	167
Bbv12I	GWGCWC	2 cut(s)	141, 1005
BbvI	GCAGC	8 cut(s)	7, 82, 223, 229, 433, 470, 601, 1285
BccI	CCATC	2 cut(s)	78, 385
BciT130I	CCWGG	3 cut(s)	300, 680, 958
BclI	TGATCA	3 cut(s)	3, 487, 1317
BcnI	CCSGG	2 cut(s)	1112, 1113
BfaI	CTAG	6 cut(s)	416, 647, 932, 1025, 1134, 1218
BfmI	CTRYAG	1 cut(s)	1047
BfuAI	ACCTGC	1 cut(s)	639
BglII	AGATCT	1 cut(s)	412
Bme1390I	CCNGG	5 cut(s)	300, 680, 958, 1112, 1113
Bme18I	GGWCC	1 cut(s)	229
BmeT110I	CYCGRG	2 cut(s)	48, 1111
BmgT120I	GGNCC	1 cut(s)	229
BmiI	GGNNCC	3 cut(s)	182, 374, 438
BmrFI	CCNGG	5 cut(s)	300, 680, 958, 1112, 1113
BmsI	GCATC	4 cut(s)	94, 118, 218, 514
Bpu14I	TTCGAA	1 cut(s)	1169
BpuMI	CCSGG	2 cut(s)	1112, 1113
BsaBI	GATNNNNATC	1 cut(s)	539
BsaJI	CCNNGG	6 cut(s)	132, 184, 679, 957, 967, 1111
BsaWI	WCCGGW	3 cut(s)	361, 793, 997
Bsc4I	CCNNNNNNNGG	2 cut(s)	368, 850
Bse118I	RCCGGY	2 cut(s)	241, 793
Bse1I	ACTGG	3 cut(s)	151, 511, 991
Bse3DI	GCAATG	1 cut(s)	36
Bse8I	GATNNNNATC	1 cut(s)	539
BseAI	TCCGGA	1 cut(s)	361
BseBI	CCWGG	3 cut(s)	300, 680, 958
BseDI	CCNNGG	6 cut(s)	132, 184, 679, 957, 967, 1111
BseGI	GGATG	2 cut(s)	403, 1088
BseJI	GATNNNNATC	1 cut(s)	539
BseLI	CCNNNNNNNGG	2 cut(s)	368, 850
BseMI	GCAATG	1 cut(s)	36
BseMII	CTCAG	1 cut(s)	872
BseNI	ACTGG	3 cut(s)	151, 511, 991
BseRI	GAGGAG	3 cut(s)	336, 827, 845
BseSI	GKGCMC	2 cut(s)	82, 141
BseXI	GCAGC	8 cut(s)	7, 82, 223, 229, 433, 470, 601, 1285
BsgI	GTGCAG	2 cut(s)	222, 236
Bsh1236I	CGCG	3 cut(s)	274, 1159, 1205
Bsh1285I	CGRYCG	2 cut(s)	590, 998
BshFI	GGCC	4 cut(s)	203, 311, 1045, 1216
BshNI	GGYRCC	1 cut(s)	372
BshTI	ACCGGT	1 cut(s)	793
BsiEI	CGRYCG	2 cut(s)	590, 998
BsiHKAI	GWGCWC	2 cut(s)	141, 1005
BsiHKCI	CYCGRG	2 cut(s)	48, 1111
BsiSI	CCGG	5 cut(s)	242, 362, 794, 998, 1112
BslFI	GGGAC	1 cut(s)	657
BslI	CCNNNNNNNGG	2 cut(s)	368, 850
BsmFI	GGGAC	1 cut(s)	657
BsnI	GGCC	4 cut(s)	203, 311, 1045, 1216
BsoBI	CYCGRG	2 cut(s)	48, 1111
Bsp119I	TTCGAA	1 cut(s)	1169
Bsp1286I	GDGCHC	4 cut(s)	82, 141, 859, 1005
Bsp13I	TCCGGA	1 cut(s)	361
Bsp143I	GATC	8 cut(s)	3, 412, 487, 517, 540, 750, 775, 1317
BspACI	CCGC	5 cut(s)	227, 605, 1205, 1213, 1295
BspANI	GGCC	4 cut(s)	203, 311, 1045, 1216
BspCNI	CTCAG	1 cut(s)	871
BspEI	TCCGGA	1 cut(s)	361
BspFNI	CGCG	3 cut(s)	274, 1159, 1205
BspHI	TCATGA	1 cut(s)	1314
BspLI	GGNNCC	3 cut(s)	182, 374, 438
BspMI	ACCTGC	1 cut(s)	639
BspT104I	TTCGAA	1 cut(s)	1169
BspT107I	GGYRCC	1 cut(s)	372
BsrDI	GCAATG	1 cut(s)	36
BsrFI	RCCGGY	2 cut(s)	241, 793
BsrI	ACTGG	3 cut(s)	151, 511, 991
BssAI	RCCGGY	2 cut(s)	241, 793
BssECI	CCNNGG	6 cut(s)	132, 184, 679, 957, 967, 1111
BssMI	GATC	8 cut(s)	3, 412, 487, 517, 540, 750, 775, 1317
BssSI	CACGAG	1 cut(s)	167
BssT1I	CCWWGG	2 cut(s)	184, 967
Bst2BI	CACGAG	1 cut(s)	167
Bst2UI	CCWGG	3 cut(s)	300, 680, 958
Bst4CI	ACNGT	2 cut(s)	588, 1164
Bst6I	CTCTTC	5 cut(s)	525, 711, 822, 902, 1111
BstBI	TTCGAA	1 cut(s)	1169
BstC8I	GCNNGC	5 cut(s)	55, 160, 603, 964, 1022
BstDEI	CTNAG	1 cut(s)	858
BstF5I	GGATG	2 cut(s)	403, 1088
BstFNI	CGCG	3 cut(s)	274, 1159, 1205
BstHHI	GCGC	2 cut(s)	267, 274
BstKTI	GATC	8 cut(s)	6, 415, 490, 520, 543, 753, 778, 1320
BstMBI	GATC	8 cut(s)	3, 412, 487, 517, 540, 750, 775, 1317
BstMCI	CGRYCG	2 cut(s)	590, 998
BstMWI	GCNNNNNNNGC	8 cut(s)	26, 104, 233, 242, 271, 611, 1156, 1295
BstNI	CCWGG	3 cut(s)	300, 680, 958
BstSCI	CCNGG	5 cut(s)	298, 678, 956, 1110, 1111
BstSFI	CTRYAG	1 cut(s)	1047
BstSLI	GKGCMC	2 cut(s)	82, 141
BstUI	CGCG	3 cut(s)	274, 1159, 1205
BstV1I	GCAGC	8 cut(s)	7, 82, 223, 229, 433, 470, 601, 1285
BstX2I	RGATCY	1 cut(s)	412
BstYI	RGATCY	1 cut(s)	412
BsuRI	GGCC	4 cut(s)	203, 311, 1045, 1216
BtgZI	GCGATG	1 cut(s)	1119
BtsCI	GGATG	2 cut(s)	403, 1088
BtsI	GCAGTG	1 cut(s)	951
BtsIMutI	CAGTG	1 cut(s)	951
BveI	ACCTGC	1 cut(s)	639
Cac8I	GCNNGC	5 cut(s)	55, 160, 603, 964, 1022
CciI	TCATGA	1 cut(s)	1314
CfoI	GCGC	2 cut(s)	267, 274
Cfr10I	RCCGGY	2 cut(s)	241, 793
Cfr13I	GGNCC	1 cut(s)	229
Cfr9I	CCCGGG	1 cut(s)	1111
CseI	GACGC	1 cut(s)	1165
Csp6I	GTAC	3 cut(s)	791, 797, 1138
CspAI	ACCGGT	1 cut(s)	793
CviAII	CATG	5 cut(s)	850, 883, 1033, 1067, 1315
CviQI	GTAC	3 cut(s)	791, 797, 1138
DdeI	CTNAG	1 cut(s)	858
DpnI	GATC	8 cut(s)	5, 414, 489, 519, 542, 752, 777, 1319
DpnII	GATC	8 cut(s)	3, 412, 487, 517, 540, 750, 775, 1317
EaeI	YGGCCR	1 cut(s)	309
Eam1104I	CTCTTC	5 cut(s)	525, 711, 822, 902, 1111
EarI	CTCTTC	5 cut(s)	525, 711, 822, 902, 1111
EciI	GGCGGA	1 cut(s)	620
Ecl136II	GAGCTC	1 cut(s)	1003
Eco130I	CCWWGG	2 cut(s)	184, 967
Eco24I	GRGCYC	2 cut(s)	859, 1005
Eco47I	GGWCC	1 cut(s)	229
Eco53kI	GAGCTC	1 cut(s)	1003
Eco88I	CYCGRG	2 cut(s)	48, 1111
EcoICRI	GAGCTC	1 cut(s)	1003
EcoRII	CCWGG	3 cut(s)	298, 678, 956
EcoT14I	CCWWGG	2 cut(s)	184, 967
EcoT22I	ATGCAT	1 cut(s)	211
EcoT38I	GRGCYC	2 cut(s)	859, 1005
ErhI	CCWWGG	2 cut(s)	184, 967
FaeI	CATG	5 cut(s)	853, 886, 1036, 1070, 1318
FaqI	GGGAC	1 cut(s)	657
FatI	CATG	5 cut(s)	849, 882, 1032, 1066, 1314
FauNDI	CATATG	1 cut(s)	920
FbaI	TGATCA	3 cut(s)	3, 487, 1317
FokI	GGATG	2 cut(s)	410, 1095
FriOI	GRGCYC	2 cut(s)	859, 1005
FspBI	CTAG	6 cut(s)	416, 647, 932, 1025, 1134, 1218
GlaI	GCGC	2 cut(s)	266, 273
HaeIII	GGCC	4 cut(s)	203, 311, 1045, 1216
HapII	CCGG	5 cut(s)	242, 362, 794, 998, 1112
HgaI	GACGC	1 cut(s)	1165
HhaI	GCGC	2 cut(s)	267, 274
Hin1II	CATG	5 cut(s)	853, 886, 1036, 1070, 1318
Hin6I	GCGC	2 cut(s)	265, 272
HinP1I	GCGC	2 cut(s)	265, 272
HinfI	GANTC	5 cut(s)	303, 675, 879, 1073, 1233
HpaII	CCGG	5 cut(s)	242, 362, 794, 998, 1112
HphI	GGTGA	1 cut(s)	82
Hpy166II	GTNNAC	1 cut(s)	139
Hpy188I	TCNGA	3 cut(s)	545, 592, 861
Hpy188III	TCNNGA	5 cut(s)	48, 362, 416, 521, 1315
Hpy8I	GTNNAC	1 cut(s)	139
HpyAV	CCTTC	6 cut(s)	703, 722, 756, 829, 1010, 1146
HpyCH4III	ACNGT	2 cut(s)	588, 1164
HpyCH4IV	ACGT	1 cut(s)	1250
HpyF10VI	GCNNNNNNNGC	8 cut(s)	26, 104, 233, 242, 271, 611, 1156, 1295
HpyF3I	CTNAG	1 cut(s)	858
HpySE526I	ACGT	1 cut(s)	1250
Hsp92II	CATG	5 cut(s)	853, 886, 1036, 1070, 1318
HspAI	GCGC	2 cut(s)	265, 272
Kpn2I	TCCGGA	1 cut(s)	361
Ksp22I	TGATCA	3 cut(s)	3, 487, 1317
Kzo9I	GATC	8 cut(s)	3, 412, 487, 517, 540, 750, 775, 1317
LmnI	GCTCC	5 cut(s)	186, 598, 762, 1000, 1294
Lsp1109I	GCAGC	8 cut(s)	7, 82, 223, 229, 433, 470, 601, 1285
LweI	GCATC	4 cut(s)	94, 118, 218, 514
MaeI	CTAG	6 cut(s)	416, 647, 932, 1025, 1134, 1218
MaeII	ACGT	1 cut(s)	1250
MaeIII	GTNAC	1 cut(s)	662
MalI	GATC	8 cut(s)	5, 414, 489, 519, 542, 752, 777, 1319
MboI	GATC	8 cut(s)	3, 412, 487, 517, 540, 750, 775, 1317
MboII	GAAGA	9 cut(s)	422, 542, 728, 839, 919, 925, 1128, 1183, 1186
MflI	RGATCY	1 cut(s)	412
MhlI	GDGCHC	4 cut(s)	82, 141, 859, 1005
MlsI	TGGCCA	1 cut(s)	311
MluCI	AATT	5 cut(s)	385, 441, 568, 737, 1053
MluNI	TGGCCA	1 cut(s)	311
MlyI	GAGTC	1 cut(s)	684
MmeI	TCCRAC	1 cut(s)	570
Mox20I	TGGCCA	1 cut(s)	311
Mph1103I	ATGCAT	1 cut(s)	211
MroI	TCCGGA	1 cut(s)	361
MscI	TGGCCA	1 cut(s)	311
MseI	TTAA	2 cut(s)	741, 1239
MslI	CAYNNNNRTG	1 cut(s)	1037
Msp20I	TGGCCA	1 cut(s)	311
MspA1I	CMGCKG	2 cut(s)	220, 788
MspI	CCGG	5 cut(s)	242, 362, 794, 998, 1112
MspR9I	CCNGG	5 cut(s)	300, 680, 958, 1112, 1113
MvaI	CCWGG	3 cut(s)	300, 680, 958
MvnI	CGCG	3 cut(s)	274, 1159, 1205
MwoI	GCNNNNNNNGC	8 cut(s)	26, 104, 233, 242, 271, 611, 1156, 1295
NciI	CCSGG	2 cut(s)	1112, 1113
NdeI	CATATG	1 cut(s)	920
NdeII	GATC	8 cut(s)	3, 412, 487, 517, 540, 750, 775, 1317
NlaIII	CATG	5 cut(s)	853, 886, 1036, 1070, 1318
NlaIV	GGNNCC	3 cut(s)	182, 374, 438
NmeAIII	GCCGAG	2 cut(s)	122, 487
NmuCI	GTSAC	1 cut(s)	662
NsiI	ATGCAT	1 cut(s)	211
NspV	TTCGAA	1 cut(s)	1169
PaeR7I	CTCGAG	1 cut(s)	48
PagI	TCATGA	1 cut(s)	1314
PfeI	GAWTC	4 cut(s)	303, 879, 1073, 1233
PflFI	GACNNNGTC	1 cut(s)	1249
PinAI	ACCGGT	1 cut(s)	793
PleI	GAGTC	1 cut(s)	683
PpsI	GAGTC	1 cut(s)	683
Psp124BI	GAGCTC	1 cut(s)	1005
Psp6I	CCWGG	3 cut(s)	298, 678, 956
PspGI	CCWGG	3 cut(s)	298, 678, 956
PspN4I	GGNNCC	3 cut(s)	182, 374, 438
PspPI	GGNCC	1 cut(s)	229
PsuI	RGATCY	1 cut(s)	412
PsyI	GACNNNGTC	1 cut(s)	1249
PvuII	CAGCTG	2 cut(s)	220, 788
RsaI	GTAC	3 cut(s)	792, 798, 1139
RsaNI	GTAC	3 cut(s)	791, 797, 1138
RseI	CAYNNNNRTG	1 cut(s)	1037
SacI	GAGCTC	1 cut(s)	1005
SaqAI	TTAA	2 cut(s)	741, 1239
Sau3AI	GATC	8 cut(s)	3, 412, 487, 517, 540, 750, 775, 1317
Sau96I	GGNCC	1 cut(s)	229
SchI	GAGTC	1 cut(s)	684
ScrFI	CCNGG	5 cut(s)	300, 680, 958, 1112, 1113
SduI	GDGCHC	4 cut(s)	82, 141, 859, 1005
SfaNI	GCATC	4 cut(s)	94, 118, 218, 514
SfcI	CTRYAG	1 cut(s)	1047
Sfr274I	CTCGAG	1 cut(s)	48
SfuI	TTCGAA	1 cut(s)	1169
SinI	GGWCC	1 cut(s)	229
SlaI	CTCGAG	1 cut(s)	48
SmaI	CCCGGG	1 cut(s)	1113
SmiMI	CAYNNNNRTG	1 cut(s)	1037
SmlI	CTYRAG	1 cut(s)	48
SmoI	CTYRAG	1 cut(s)	48
Sse9I	AATT	5 cut(s)	385, 441, 568, 737, 1053
SsiI	CCGC	5 cut(s)	227, 605, 1205, 1213, 1295
SspI	AATATT	1 cut(s)	475
SspMI	CTAG	6 cut(s)	416, 647, 932, 1025, 1134, 1218
SstI	GAGCTC	1 cut(s)	1005
StyD4I	CCNGG	5 cut(s)	298, 678, 956, 1110, 1111
StyI	CCWWGG	2 cut(s)	184, 967
TaaI	ACNGT	2 cut(s)	588, 1164
TaiI	ACGT	1 cut(s)	1253
TaqI	TCGA	7 cut(s)	49, 522, 749, 994, 1093, 1169, 1231
TasI	AATT	5 cut(s)	385, 441, 568, 737, 1053
TauI	GCSGC	2 cut(s)	1216, 1298
TfiI	GAWTC	4 cut(s)	303, 879, 1073, 1233
Tru1I	TTAA	2 cut(s)	741, 1239
Tru9I	TTAA	2 cut(s)	741, 1239
TscAI	CASTG	1 cut(s)	958
TseFI	GTSAC	1 cut(s)	662
TseI	GCWGC	8 cut(s)	20, 95, 217, 236, 421, 458, 614, 1298
Tsp45I	GTSAC	1 cut(s)	662
TspDTI	ATGAA	6 cut(s)	180, 423, 871, 920, 1046, 1238
TspGWI	ACGGA	1 cut(s)	23
TspMI	CCCGGG	1 cut(s)	1111
TspRI	CASTG	1 cut(s)	958
Tth111I	GACNNNGTC	1 cut(s)	1249
VneI	GTGCAC	1 cut(s)	137
VpaK11BI	GGWCC	1 cut(s)	229
XapI	RAATTY	3 cut(s)	385, 737, 1053
XbaI	TCTAGA	1 cut(s)	415
XhoI	CTCGAG	1 cut(s)	48
XmaI	CCCGGG	1 cut(s)	1111
XspI	CTAG	6 cut(s)	416, 647, 932, 1025, 1134, 1218
Zsp2I	ATGCAT	1 cut(s)	211

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.