MD06G1222400.v1.1
TCP Family

T-complex protein 1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016221
Physical Location & Seq
Reverse (-)
35391379 .. 35392983
1605 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1222400.v1.1.491

Sequence Viewer

Length: 1605 bp
ATGGCTTCCCCGGCAGTCATCTCCCAACCGCGCTCCTCCAAGACCGAGTCTTATGTCGATAACAAGCGCAAGGAGGACGTCCGCCAGGCCAACATCACCGCCGCTCGCGCAGTCGCCGATGCCGTCCGAACCAGCCTGGGACCCAAGGGCATGGACAAGATGATCTCCACCGCCAACGGCGAGGTTATCATCACCAACGACGGCGCCACGATTCTTAACAAGATGGAGGTCCTCCAACCGGCCGCCAAGATGCTTGTCGAGCTTTCCAAGTCCCAGGACGCAGCCGCCGGTGACGGCACCACTACCGTCGTCGTCATTGCCGGCGCTCTCCTGAAACAGTGCCAGGTCCTTCTGTCCCACGGCATTCACCCGACTGTGATCTCCGACTCTCTCTATAAGGCCTCCACCAAAGCCGTCGACGTCTTGACCGCCATGGCCGTCCCCGTCGAGCTCACGGACCGTGATTCGCTTGTCAAGTCTGCAAGCACATCGCTCAACAGCAAGGTGGTGAGCCAGTACTCGACTCTGCTCGCGCCGATGGCCGTGGACGCTGTGCTCTCCGTGGTGGACCCCGCGAAGCCAGAGGTGGTGGACCTGAGGGATATCAGGATCGTGAAGAAGCTTGGAGGAACCGTGGACGATACTGAGACGGTGAAGGGACTTGTTTTCGATAAGAAGGCGAGCCACGCTGCTGGTGGGCCGACAAGGATGGAGAACGCCAAGATTGCTGTGATTCAGTTCCAGATTTCGCCTCCAAAAACCGATATCGAGCAGAGCATTGTCGTCTCGGATTATACTCAGATGGATAGGATTTTGAAGGAAGAGAGGAATTACATTCTGGGTATGATTAAGAAGATCAAGGCGACCGGTTGCAACGTTTTGCTGATTCAGAAGAGTATTCTGAGAGATGCAGTTACTGATTTGTCCTTGCATTACTTGGCGAAAGCGAAGATCTTGGTGATCAAGGATGTGGAGAGAGATGAGATTGAGTTCATCACCAAGACTCTGAATTGCTTGCCCATTGCGAATATCGAGCATTTCCGGGCAGAGAAATTGGGTTATGCTGATCTGGTTGAGGAGGTTTCACTTGGTGATGGGAAGATTGTGAAGATAACGGGGATTCAAAACATGGGTCGGACCACCACTGTGCTGGTTCGCGGGTCAAACCAGCTGGTGCTAGATGAGGCGGAGCGGAGCCTGCACGATGCTCTGTGTGTGGTGAGGTGTTTGGTGAGCAAAAGGTTTTTGATTGCAGGTGGCGGTGCGCCAGAGATTGAGCTGTCGAGGCAGTTGGGGGCGTGGGCTAAGGTGCTGCAGGGGATGGAGGGTTATTGTGTGAAGTCGTTTGCTGAGGCGCTCGAGGTTGTTCCCTATACGCTGGCTGAGAATGCCGGGTTGAACCCAATTTCAATTGTGACTGAGCTGAGGAACCGGCACGCACAGGGAGAGATCAACACCGGGATTAATGTGAGGAAGGGGCAGATTACCAACATCTTGGAGGAGAATGTGGTGCAGCCATTGCTCGTCAGCACAAGTGCCATCACTCTGGCTGCCGAGTGTGTGCGGATGATTTTGAAGATCGATGACATAGTTACAGTAAGATAG

Protein Analysis

535

Amino Acids

57.64

Weight (kDa)

7.05

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cpn60_TCP1 PF00118 38 - 532 4.2e-163 TCP-1/cpn60 chaperonin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016221)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18190
fragaria_vesca FvH4_7g06380
malus_domestica MD06G1222400.v1.1 MD14G1231500.v1.1
prunus_persica Prupe.5G227000_v2.0.a1
pyrus_communis pycom14g19270
rosa_chinensis RchiOBHm_Chr1g0334481
rosa_laevigata RLG00000029450
rosa_multiflora Rmu_ssc0000404.1_g000012
rosa_roxburghii Rroxscaffold_4G00316810
rosa_rugosa Rorug01G0114200
rosa_samantha Rh1AG139400 Rh1BG105800 Rh1DG143300
rosa_wichuraiana Rw1G011490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1244
AatII GACGTC 2 cut(s) 81, 423
Acc36I ACCTGC 1 cut(s) 1244
AccB1I GGYRCC 2 cut(s) 203, 296
AccBSI CCGCTC 2 cut(s) 104, 1192
AccI GTMKAC 1 cut(s) 417
AccII CGCG 5 cut(s) 31, 108, 533, 575, 1158
AclI AACGTT 1 cut(s) 876
AclWI GGATC 1 cut(s) 617
AcoI YGGCCR 3 cut(s) 240, 435, 540
AcyI GRCGYC 3 cut(s) 78, 204, 420
AdeI CACNNNGTG 1 cut(s) 1091
AfaI GTAC 1 cut(s) 518
AfiI CCNNNNNNNGG 1 cut(s) 238
AgeI ACCGGT 1 cut(s) 866
AgsI TTSAA 5 cut(s) 817, 1124, 1399, 1410, 1576
AjnI CCWGG 4 cut(s) 84, 135, 273, 342
AleI CACNNNNGTG 1 cut(s) 1145
AluBI AGCT 6 cut(s) 262, 451, 622, 1171, 1279, 1423
AluI AGCT 6 cut(s) 262, 451, 622, 1171, 1279, 1423
Alw21I GWGCWC 2 cut(s) 453, 558
Alw26I GTCTC 2 cut(s) 641, 790
AlwI GGATC 1 cut(s) 617
AlwNI CAGNNNCTG 1 cut(s) 917
Ama87I CYCGRG 1 cut(s) 1358
AoxI GGCC 6 cut(s) 87, 240, 399, 435, 540, 698
ApeKI GCWGC 5 cut(s) 281, 689, 1312, 1513, 1550
ArsI GACNNNNNNTTYG 2 cut(s) 1117, 1149
AseI ATTAAT 1 cut(s) 1464
AsiGI ACCGGT 1 cut(s) 866
AspLEI GCGC 8 cut(s) 33, 69, 110, 206, 326, 535, 1267, 1357
AspS9I GGNCC 8 cut(s) 140, 229, 346, 457, 568, 592, 698, 1137
AsuC2I CCSGG 4 cut(s) 11, 1043, 1393, 1459
AvaI CYCGRG 1 cut(s) 1358
AvaII GGWCC 7 cut(s) 140, 229, 346, 457, 568, 592, 1137
AxyI CCTNAGG 1 cut(s) 596
BanI GGYRCC 2 cut(s) 203, 296
BanII GRGCYC 1 cut(s) 453
Bbv12I GWGCWC 2 cut(s) 453, 558
BbvCI CCTCAGC 2 cut(s) 1350, 1424
BbvI GCAGC 5 cut(s) 293, 676, 1299, 1525, 1537
BccI CCATC 7 cut(s) 217, 532, 703, 796, 1088, 1315, 1547
BceAI ACGGC 8 cut(s) 107, 193, 217, 310, 376, 398, 422, 527
BcgI CGANNNNNNTGC 2 cut(s) 471, 505
BciT130I CCWGG 4 cut(s) 86, 137, 275, 344
BclI TGATCA 1 cut(s) 960
BcnI CCSGG 4 cut(s) 11, 1043, 1393, 1459
BcoDI GTCTC 2 cut(s) 641, 790
BfaI CTAG 1 cut(s) 1178
BfmI CTRYAG 1 cut(s) 1313
BfoI RGCGCY 3 cut(s) 207, 327, 1358
BfuAI ACCTGC 1 cut(s) 1244
BglII AGATCT 1 cut(s) 951
BisI GCNGC 8 cut(s) 102, 243, 282, 285, 690, 1313, 1514, 1551
BlsI GCNGC 8 cut(s) 103, 244, 283, 286, 691, 1314, 1515, 1552
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 8 cut(s) 11, 86, 137, 275, 344, 1043, 1393, 1459
Bme18I GGWCC 7 cut(s) 140, 229, 346, 457, 568, 592, 1137
BmeT110I CYCGRG 1 cut(s) 1358
BmgT120I GGNCC 8 cut(s) 140, 229, 346, 457, 568, 592, 698, 1137
BmiI GGNNCC 8 cut(s) 141, 142, 205, 298, 570, 631, 1196, 1430
BmrFI CCNGG 8 cut(s) 11, 86, 137, 275, 344, 1043, 1393, 1459
BmsI GCATC 4 cut(s) 109, 240, 898, 1195
Bpu10I CCTNAGC 3 cut(s) 1305, 1350, 1424
BpuMI CCSGG 4 cut(s) 11, 1043, 1393, 1459
Bsa29I ATCGAT 1 cut(s) 1581
BsaHI GRCGYC 3 cut(s) 78, 204, 420
BsaJI CCNNGG 9 cut(s) 9, 136, 144, 273, 358, 432, 543, 561, 633
BsaWI WCCGGW 1 cut(s) 866
BsaXI ACNNNNNCTCC 2 cut(s) 618, 648
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse118I RCCGGY 5 cut(s) 238, 287, 320, 866, 1431
Bse1I ACTGG 1 cut(s) 514
Bse21I CCTNAGG 1 cut(s) 596
Bse3DI GCAATG 3 cut(s) 315, 1020, 1517
BseBI CCWGG 4 cut(s) 86, 137, 275, 344
BseCI ATCGAT 1 cut(s) 1581
BseDI CCNNGG 9 cut(s) 9, 136, 144, 273, 358, 432, 543, 561, 633
BseGI GGATG 4 cut(s) 714, 973, 1326, 1572
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMI GCAATG 3 cut(s) 315, 1020, 1517
BseMII CTCAG 8 cut(s) 587, 636, 812, 893, 1341, 1374, 1410, 1415
BseNI ACTGG 1 cut(s) 514
BseRI GAGGAG 3 cut(s) 25, 1091, 1514
BseX3I CGGCCG 1 cut(s) 240
BseXI GCAGC 5 cut(s) 293, 676, 1299, 1525, 1537
BsgI GTGCAG 2 cut(s) 1184, 1532
Bsh1236I CGCG 5 cut(s) 31, 108, 533, 575, 1158
Bsh1285I CGRYCG 2 cut(s) 243, 867
BshFI GGCC 6 cut(s) 89, 242, 401, 437, 542, 700
BshNI GGYRCC 2 cut(s) 203, 296
BshTI ACCGGT 1 cut(s) 866
BshVI ATCGAT 1 cut(s) 1581
BsiEI CGRYCG 2 cut(s) 243, 867
BsiHKAI GWGCWC 2 cut(s) 453, 558
BsiHKCI CYCGRG 1 cut(s) 1358
BsiSI CCGG 9 cut(s) 11, 239, 288, 321, 867, 1042, 1392, 1432, 1458
BslFI GGGAC 5 cut(s) 153, 256, 340, 425, 672
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 2 cut(s) 641, 790
BsmBI CGTCTC 2 cut(s) 641, 790
BsmFI GGGAC 5 cut(s) 153, 256, 340, 425, 672
BsmI GAATGC 2 cut(s) 363, 1393
BsnI GGCC 6 cut(s) 89, 242, 401, 437, 542, 700
BsoBI CYCGRG 1 cut(s) 1358
Bsp1286I GDGCHC 2 cut(s) 453, 558
Bsp143I GATC 9 cut(s) 162, 378, 609, 855, 951, 960, 1066, 1449, 1578
Bsp19I CCATGG 1 cut(s) 432
BspANI GGCC 6 cut(s) 89, 242, 401, 437, 542, 700
BspCNI CTCAG 8 cut(s) 588, 637, 811, 894, 1342, 1375, 1411, 1416
BspDI ATCGAT 1 cut(s) 1581
BspFNI CGCG 5 cut(s) 31, 108, 533, 575, 1158
BspLI GGNNCC 8 cut(s) 141, 142, 205, 298, 570, 631, 1196, 1430
BspMAI CTGCAG 1 cut(s) 1317
BspMI ACCTGC 1 cut(s) 1244
BspPI GGATC 1 cut(s) 617
BspT107I GGYRCC 2 cut(s) 203, 296
BsrBI CCGCTC 2 cut(s) 104, 1192
BsrDI GCAATG 3 cut(s) 315, 1020, 1517
BsrFI RCCGGY 5 cut(s) 238, 287, 320, 866, 1431
BsrI ACTGG 1 cut(s) 514
BssAI RCCGGY 5 cut(s) 238, 287, 320, 866, 1431
BssECI CCNNGG 9 cut(s) 9, 136, 144, 273, 358, 432, 543, 561, 633
BssMI GATC 9 cut(s) 162, 378, 609, 855, 951, 960, 1066, 1449, 1578
BssNI GRCGYC 3 cut(s) 78, 204, 420
BssT1I CCWWGG 2 cut(s) 144, 432
Bst2UI CCWGG 4 cut(s) 86, 137, 275, 344
Bst4CI ACNGT 8 cut(s) 307, 339, 376, 461, 634, 652, 1147, 1597
Bst6I CTCTTC 2 cut(s) 816, 887
BstACI GRCGYC 3 cut(s) 78, 204, 420
BstAPI GCANNNNNTGC 1 cut(s) 1519
BstC8I GCNNGC 9 cut(s) 106, 322, 484, 531, 682, 1016, 1199, 1380, 1437
BstDEI CTNAG 9 cut(s) 596, 645, 798, 902, 1305, 1350, 1383, 1419, 1424
BstDSI CCRYGG 5 cut(s) 358, 432, 543, 561, 633
BstF5I GGATG 4 cut(s) 714, 973, 1326, 1572
BstFNI CGCG 5 cut(s) 31, 108, 533, 575, 1158
BstH2I RGCGCY 3 cut(s) 207, 327, 1358
BstHHI GCGC 8 cut(s) 33, 69, 110, 206, 326, 535, 1267, 1357
BstKTI GATC 9 cut(s) 165, 381, 612, 858, 954, 963, 1069, 1452, 1581
BstMAI GTCTC 2 cut(s) 641, 790
BstMBI GATC 9 cut(s) 162, 378, 609, 855, 951, 960, 1066, 1449, 1578
BstMCI CGRYCG 2 cut(s) 243, 867
BstNI CCWGG 4 cut(s) 86, 137, 275, 344
BstSCI CCNGG 8 cut(s) 9, 84, 135, 273, 342, 1041, 1391, 1457
BstSFI CTRYAG 1 cut(s) 1313
BstUI CGCG 5 cut(s) 31, 108, 533, 575, 1158
BstV1I GCAGC 5 cut(s) 293, 676, 1299, 1525, 1537
BstX2I RGATCY 1 cut(s) 951
BstXI CCANNNNNNTGG 5 cut(s) 151, 692, 1150, 1495, 1546
BstYI RGATCY 1 cut(s) 951
BstZI CGGCCG 1 cut(s) 240
Bsu15I ATCGAT 1 cut(s) 1581
Bsu36I CCTNAGG 1 cut(s) 596
BsuRI GGCC 6 cut(s) 89, 242, 401, 437, 542, 700
BsuTUI ATCGAT 1 cut(s) 1581
BtgI CCRYGG 5 cut(s) 358, 432, 543, 561, 633
BtgZI GCGATG 1 cut(s) 474
BtsCI GGATG 4 cut(s) 714, 973, 1326, 1572
BtsIMutI CAGTG 2 cut(s) 344, 1143
BveI ACCTGC 1 cut(s) 1244
Cac8I GCNNGC 9 cut(s) 106, 322, 484, 531, 682, 1016, 1199, 1380, 1437
CaiI CAGNNNCTG 1 cut(s) 917
CfoI GCGC 8 cut(s) 33, 69, 110, 206, 326, 535, 1267, 1357
Cfr10I RCCGGY 5 cut(s) 238, 287, 320, 866, 1431
Cfr13I GGNCC 8 cut(s) 140, 229, 346, 457, 568, 592, 698, 1137
ClaI ATCGAT 1 cut(s) 1581
CpoI CGGWCCG 1 cut(s) 457
CseI GACGC 2 cut(s) 287, 557
Csp6I GTAC 1 cut(s) 517
CspAI ACCGGT 1 cut(s) 866
CspI CGGWCCG 1 cut(s) 457
CviAII CATG 3 cut(s) 151, 433, 1129
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 9 cut(s) 596, 645, 798, 902, 1305, 1350, 1383, 1419, 1424
DinI GGCGCC 1 cut(s) 205
DpnI GATC 9 cut(s) 164, 380, 611, 857, 953, 962, 1068, 1451, 1580
DpnII GATC 9 cut(s) 162, 378, 609, 855, 951, 960, 1066, 1449, 1578
DraIII CACNNNGTG 1 cut(s) 1091
EaeI YGGCCR 3 cut(s) 240, 435, 540
EagI CGGCCG 1 cut(s) 240
Eam1104I CTCTTC 2 cut(s) 816, 887
EarI CTCTTC 2 cut(s) 816, 887
EciI GGCGGA 2 cut(s) 71, 1202
Ecl136II GAGCTC 1 cut(s) 451
EclXI CGGCCG 1 cut(s) 240
Eco130I CCWWGG 2 cut(s) 144, 432
Eco147I AGGCCT 1 cut(s) 401
Eco24I GRGCYC 1 cut(s) 453
Eco32I GATATC 2 cut(s) 604, 766
Eco47I GGWCC 7 cut(s) 140, 229, 346, 457, 568, 592, 1137
Eco52I CGGCCG 1 cut(s) 240
Eco53kI GAGCTC 1 cut(s) 451
Eco81I CCTNAGG 1 cut(s) 596
Eco88I CYCGRG 1 cut(s) 1358
EcoICRI GAGCTC 1 cut(s) 451
EcoO109I RGGNCCY 3 cut(s) 140, 229, 346
EcoRII CCWGG 4 cut(s) 84, 135, 273, 342
EcoRV GATATC 2 cut(s) 604, 766
EcoT14I CCWWGG 2 cut(s) 144, 432
EcoT38I GRGCYC 1 cut(s) 453
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
ErhI CCWWGG 2 cut(s) 144, 432
Esp3I CGTCTC 2 cut(s) 641, 790
FaeI CATG 3 cut(s) 154, 436, 1132
FaqI GGGAC 5 cut(s) 153, 256, 340, 425, 672
FatI CATG 3 cut(s) 150, 432, 1128
FauI CCCGC 2 cut(s) 580, 1151
FbaI TGATCA 1 cut(s) 960
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 8 cut(s) 102, 243, 282, 285, 690, 1313, 1514, 1551
FokI GGATG 4 cut(s) 721, 980, 1333, 1579
FriOI GRGCYC 1 cut(s) 453
Fsp4HI GCNGC 8 cut(s) 102, 243, 282, 285, 690, 1313, 1514, 1551
FspBI CTAG 1 cut(s) 1178
GlaI GCGC 8 cut(s) 32, 68, 109, 205, 325, 534, 1266, 1356
GluI GCNGC 8 cut(s) 102, 243, 282, 285, 690, 1313, 1514, 1551
HaeII RGCGCY 3 cut(s) 207, 327, 1358
HaeIII GGCC 6 cut(s) 89, 242, 401, 437, 542, 700
HapII CCGG 9 cut(s) 11, 239, 288, 321, 867, 1042, 1392, 1432, 1458
HgaI GACGC 2 cut(s) 287, 557
HhaI GCGC 8 cut(s) 33, 69, 110, 206, 326, 535, 1267, 1357
Hin1I GRCGYC 3 cut(s) 78, 204, 420
Hin1II CATG 3 cut(s) 154, 436, 1132
Hin6I GCGC 8 cut(s) 31, 67, 108, 204, 324, 533, 1265, 1355
HinP1I GCGC 8 cut(s) 31, 67, 108, 204, 324, 533, 1265, 1355
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HindIII AAGCTT 1 cut(s) 620
HinfI GANTC 9 cut(s) 47, 211, 386, 464, 523, 733, 886, 1003, 1120
HpaII CCGG 9 cut(s) 11, 239, 288, 321, 867, 1042, 1392, 1432, 1458
Hpy166II GTNNAC 5 cut(s) 418, 547, 568, 592, 637
Hpy188I TCNGA 8 cut(s) 128, 385, 790, 801, 891, 903, 1008, 1137
Hpy188III TCNNGA 5 cut(s) 331, 424, 607, 613, 742
Hpy8I GTNNAC 5 cut(s) 418, 547, 568, 592, 637
Hpy99I CGWCG 6 cut(s) 203, 311, 314, 419, 422, 449
HpyAV CCTTC 5 cut(s) 359, 649, 670, 811, 1468
HpyCH4III ACNGT 8 cut(s) 307, 339, 376, 461, 634, 652, 1147, 1597
HpyCH4IV ACGT 3 cut(s) 78, 420, 876
HpyCH4V TGCA 8 cut(s) 482, 873, 911, 931, 1201, 1253, 1315, 1513
HpyF3I CTNAG 9 cut(s) 596, 645, 798, 902, 1305, 1350, 1383, 1419, 1424
HpySE526I ACGT 3 cut(s) 78, 420, 876
Hsp92I GRCGYC 3 cut(s) 78, 204, 420
Hsp92II CATG 3 cut(s) 154, 436, 1132
HspAI GCGC 8 cut(s) 31, 67, 108, 204, 324, 533, 1265, 1355
KasI GGCGCC 1 cut(s) 203
KflI GGGWCCC 1 cut(s) 140
KroI GCCGGC 1 cut(s) 320
KroNI GCCGGC 1 cut(s) 322
Ksp22I TGATCA 1 cut(s) 960
Kzo9I GATC 9 cut(s) 162, 378, 609, 855, 951, 960, 1066, 1449, 1578
LmnI GCTCC 3 cut(s) 38, 1189, 1194
Lsp1109I GCAGC 5 cut(s) 293, 676, 1299, 1525, 1537
LweI GCATC 4 cut(s) 109, 240, 898, 1195
MaeI CTAG 1 cut(s) 1178
MaeII ACGT 3 cut(s) 78, 420, 876
MaeIII GTNAC 4 cut(s) 290, 913, 1414, 1591
MalI GATC 9 cut(s) 164, 380, 611, 857, 953, 962, 1068, 1451, 1580
MbiI CCGCTC 2 cut(s) 104, 1192
MboI GATC 9 cut(s) 162, 378, 609, 855, 951, 960, 1066, 1449, 1578
MboII GAAGA 8 cut(s) 628, 833, 865, 904, 961, 1111, 1120, 1588
MfeI CAATTG 1 cut(s) 1410
MflI RGATCY 1 cut(s) 951
MhlI GDGCHC 2 cut(s) 453, 558
MluCI AATT 5 cut(s) 829, 1009, 1052, 1404, 1410
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 4 cut(s) 56, 380, 517, 997
MmeI TCCRAC 3 cut(s) 259, 408, 1115
MroNI GCCGGC 1 cut(s) 320
MseI TTAA 3 cut(s) 216, 849, 1464
MslI CAYNNNNRTG 1 cut(s) 1145
MspA1I CMGCKG 1 cut(s) 1171
MspI CCGG 9 cut(s) 11, 239, 288, 321, 867, 1042, 1392, 1432, 1458
MspR9I CCNGG 8 cut(s) 11, 86, 137, 275, 344, 1043, 1393, 1459
MunI CAATTG 1 cut(s) 1410
Mva1269I GAATGC 2 cut(s) 363, 1393
MvaI CCWGG 4 cut(s) 86, 137, 275, 344
MvnI CGCG 5 cut(s) 31, 108, 533, 575, 1158
NaeI GCCGGC 1 cut(s) 322
NarI GGCGCC 1 cut(s) 204
NciI CCSGG 4 cut(s) 11, 1043, 1393, 1459
NcoI CCATGG 1 cut(s) 432
NdeII GATC 9 cut(s) 162, 378, 609, 855, 951, 960, 1066, 1449, 1578
NgoMIV GCCGGC 1 cut(s) 320
NlaIII CATG 3 cut(s) 154, 436, 1132
NlaIV GGNNCC 8 cut(s) 141, 142, 205, 298, 570, 631, 1196, 1430
NmeAIII GCCGAG 1 cut(s) 1579
NmuCI GTSAC 2 cut(s) 290, 1414
OliI CACNNNNGTG 1 cut(s) 1145
PaeR7I CTCGAG 1 cut(s) 1358
PaqCI CACCTGC 1 cut(s) 1244
PceI AGGCCT 1 cut(s) 401
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 2 cut(s) 363, 1393
PdiI GCCGGC 1 cut(s) 322
PfeI GAWTC 5 cut(s) 211, 464, 733, 886, 1120
PflFI GACNNNGTC 1 cut(s) 46
PinAI ACCGGT 1 cut(s) 866
PkrI GCNGC 8 cut(s) 103, 244, 283, 286, 691, 1314, 1515, 1552
PleI GAGTC 4 cut(s) 55, 380, 517, 997
PluTI GGCGCC 1 cut(s) 207
PpsI GAGTC 4 cut(s) 55, 380, 517, 997
PpuMI RGGWCCY 3 cut(s) 140, 229, 346
PshBI ATTAAT 1 cut(s) 1464
Psp124BI GAGCTC 1 cut(s) 453
Psp1406I AACGTT 1 cut(s) 876
Psp5II RGGWCCY 3 cut(s) 140, 229, 346
Psp6I CCWGG 4 cut(s) 84, 135, 273, 342
PspGI CCWGG 4 cut(s) 84, 135, 273, 342
PspN4I GGNNCC 8 cut(s) 141, 142, 205, 298, 570, 631, 1196, 1430
PspPI GGNCC 8 cut(s) 140, 229, 346, 457, 568, 592, 698, 1137
PspPPI RGGWCCY 3 cut(s) 140, 229, 346
PspXI VCTCGAGB 1 cut(s) 1358
PstI CTGCAG 1 cut(s) 1317
PstNI CAGNNNCTG 1 cut(s) 917
PsuI RGATCY 1 cut(s) 951
PsyI GACNNNGTC 1 cut(s) 46
PvuII CAGCTG 1 cut(s) 1171
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
RseI CAYNNNNRTG 1 cut(s) 1145
Rsr2I CGGWCCG 1 cut(s) 457
RsrII CGGWCCG 1 cut(s) 457
SacI GAGCTC 1 cut(s) 453
SalI GTCGAC 1 cut(s) 416
SaqAI TTAA 3 cut(s) 216, 849, 1464
SatI GCNGC 8 cut(s) 102, 243, 282, 285, 690, 1313, 1514, 1551
Sau3AI GATC 9 cut(s) 162, 378, 609, 855, 951, 960, 1066, 1449, 1578
Sau96I GGNCC 8 cut(s) 140, 229, 346, 457, 568, 592, 698, 1137
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 4 cut(s) 56, 380, 517, 997
ScrFI CCNGG 8 cut(s) 11, 86, 137, 275, 344, 1043, 1393, 1459
SduI GDGCHC 2 cut(s) 453, 558
SfaNI GCATC 4 cut(s) 109, 240, 898, 1195
SfcI CTRYAG 1 cut(s) 1313
SfoI GGCGCC 1 cut(s) 205
Sfr274I CTCGAG 1 cut(s) 1358
SgrAI CRCCGGYG 1 cut(s) 287
SgrDI CGTCGACG 1 cut(s) 416
SinI GGWCC 7 cut(s) 140, 229, 346, 457, 568, 592, 1137
SlaI CTCGAG 1 cut(s) 1358
SmiMI CAYNNNNRTG 1 cut(s) 1145
SmlI CTYRAG 1 cut(s) 1358
SmoI CTYRAG 1 cut(s) 1358
Sse9I AATT 5 cut(s) 829, 1009, 1052, 1404, 1410
SseBI AGGCCT 1 cut(s) 401
SspDI GGCGCC 1 cut(s) 203
SspMI CTAG 1 cut(s) 1178
SstI GAGCTC 1 cut(s) 453
StuI AGGCCT 1 cut(s) 401
StyD4I CCNGG 8 cut(s) 9, 84, 135, 273, 342, 1041, 1391, 1457
StyI CCWWGG 2 cut(s) 144, 432
TaaI ACNGT 8 cut(s) 307, 339, 376, 461, 634, 652, 1147, 1597
TaiI ACGT 3 cut(s) 81, 423, 879
TaqII GACCGA 1 cut(s) 59
TasI AATT 5 cut(s) 829, 1009, 1052, 1404, 1410
TatI WGTACW 1 cut(s) 516
TauI GCSGC 3 cut(s) 104, 245, 287
TfiI GAWTC 5 cut(s) 211, 464, 733, 886, 1120
Tru1I TTAA 3 cut(s) 216, 849, 1464
Tru9I TTAA 3 cut(s) 216, 849, 1464
TscAI CASTG 2 cut(s) 344, 1150
TseFI GTSAC 2 cut(s) 290, 1414
TseI GCWGC 5 cut(s) 281, 689, 1312, 1513, 1550
Tsp45I GTSAC 2 cut(s) 290, 1414
TspDTI ATGAA 1 cut(s) 982
TspGWI ACGGA 2 cut(s) 470, 550
TspRI CASTG 2 cut(s) 344, 1150
Tth111I GACNNNGTC 1 cut(s) 46
VpaK11BI GGWCC 7 cut(s) 140, 229, 346, 457, 568, 592, 1137
VspI ATTAAT 1 cut(s) 1464
XcmI CCANNNNNNNNNTGG 2 cut(s) 692, 1147
XhoI CTCGAG 1 cut(s) 1358
XmiI GTMKAC 1 cut(s) 417
XspI CTAG 1 cut(s) 1178
ZraI GACGTC 2 cut(s) 79, 421
ZrmI AGTACT 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.