Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0016221

Physical Location & Seq

Genomic Coordinates Reverse (-)

31201113 .. 31202901

Gene Structure 1789 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1231500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1749 bp

ATGGCTTCACCGGCAGTGATCTCCCAACCGCGCTCCTCCAAGACCGAGTCCTATGTCGACAACAAGCGCAAGGAGGACATCCGCCAGGCCAACATCACCGCCGCTCGAGCCGTTGCCGATGCCGTTCGAACCAGCCTGGGACCCAAGGGTATGGACAAGATGATCTCCACCGCTAACGGCGAGGTTATCATCACCAACGACGGCGCCACGATTCTTAACAAGATGGAGGTCCTCCAACCCGCCGCCAAAATGCTCGTCGAGCTTTCTAAGTCACAGGATGCTGCCGCCGGAGATGGAACCACCACCGTCGTCGTCATTGCCGGCGCTCTCCTGAAACAGTGCCAGGTCCTTCTGTCACACGGCATCCACCCGACTGTGATCTCTGACTCTCTCCACAAGGCGTCCACCAAAGCGGTAGACATCTTGACCGCCATGGCCGTCCCCGTTGAGCTCACAGACCGTGATTCGCTGGTAAAGTCTGCAAGCACATCGCTCAACAGCAAGGTGGTGAGCCAGTACTCGACTCTGCTCGCACCGATGGCCGTGGACGCCGTGCTCTCCGTGGTGGACCCGGCGAAGCCGGAGATAGTGGACCTTAGGGATATCAGGATCGTGAAGAAGCTCGGAGGAACTGTCGACGATACTGAGATGGTGAAGGGACTTGTTTTCGATAAGAAGGCGAGTCACGCCGCCGGTGGGCCGACGAGGATGGAGAATGCCAAGATTGCTGTGATTCAGTTCCAGATTTCGCCTCCGAAAACCGATATTGAGCAGAGCATTGTCATCTCGGATTATACTCAGATGGATAGGATTCTGAAGGAAGAGAGGAATTACATTCTGGGTATGATCAAGAAGATCAAGGCTACTGGATGCAACGTTTTGCTGATTCAGAAGAGTATTCTGAGAGATGCAGTTACTGATTTGTCCTTGCATTACTTGGCGAAAGCGAAGATCTTGGTGATCAAGGATGTGGAGAGAGATGAGATTGAGTTCATCACCAAGACTCTGAATTGCTTACCCATTGCGAATATCGAGCATTTCCGGGCCGAGAAACTGGGGTATGCTGATATGGTTGAGGAGGTTTCGCTTGGTGATGGGAAGATTGTGAAGATCACAGGGATTCAGAACATGGGTCGGACCACCACCGTACTGGTTCGCGGCTCGAATCAGCTGGTGCTAGATGAGGCGGAGCGGAGCCTGCACGATGCTCTTTGTGTGGTGAGGTGTTTGGTGAGCAAGAGGTTTTTGATTGCAGGTGGCGGTGCACCAGAGATTGAGCTGTCGAGGCAGTTGGGGGCGTGGGCTAAGGTGCTGCAGGGGATGGAGGGTTATTGTGTGAAGTCGTTTGCTGAGGCGCTCGAGGTTGTTCCCTATACACTGGCAGAGAATGCCGGGTTGAACCCAATTTCGATTGTGACTGAGCTGAGGAATCGCCACGCGCAGGGAGAGATCAACACCGGGATTAATGTGAGGAAGGGGCAGATTACCAACATCTTGGAGGAGAATGTGGTGCAGCCATTGCTCGTCAGCACAAGTGCCATCACTCTGGCCACCGAGTGCGTGCGAATGATTTTGAAGATTGATGACATTGTCACTTCTATTTCATTCGAGGTAGATTTCTGTTCCGAATTTTGGTATTGGTTCTGGATGTTTACTTTGGATTTGAACTTTGTTTTTGCGCTTGCTAGGCGGATGTTATGTAACCTACCTTTGCCCATTTATATGCATCTAATTAGTTTGTCGTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005832 GO:0006457 GO:0006458 GO:0008150 GO:0009987 GO:0010035 GO:0010038 GO:0032991 GO:0042221 GO:0044183 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0046686 GO:0050896 GO:0051082 GO:0061077 GO:0101031

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cpn60_TCP1

Protein Analysis

583

Amino Acids

63.59

Weight (kDa)

6.36

Isoelectric Point (pI)

37.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cpn60_TCP1	PF00118	38 - 533	5.8e-164	TCP-1/cpn60 chaperonin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18190
fragaria_vesca	FvH4_7g06380
malus_domestica	MD06G1222400.v1.1 MD14G1231500.v1.1
prunus_persica	Prupe.5G227000_v2.0.a1
pyrus_communis	pycom14g19270
rosa_chinensis	RchiOBHm_Chr1g0334481
rosa_laevigata	RLG00000029450
rosa_multiflora	Rmu_ssc0000404.1_g000012
rosa_roxburghii	Rroxscaffold_4G00316810
rosa_rugosa	Rorug01G0114200
rosa_samantha	Rh1AG139400 Rh1BG105800 Rh1DG143300
rosa_wichuraiana	Rw1G011490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1244
Acc36I	ACCTGC	1 cut(s)	1244
AccB1I	GGYRCC	1 cut(s)	203
AccBSI	CCGCTC	2 cut(s)	104, 1192
AccI	GTMKAC	3 cut(s)	57, 417, 636
AccII	CGCG	3 cut(s)	31, 1158, 1439
AclI	AACGTT	1 cut(s)	876
AclWI	GGATC	1 cut(s)	617
AcoI	YGGCCR	3 cut(s)	435, 540, 1548
AcsI	RAATTY	1 cut(s)	1628
AcuI	CTGAAG	1 cut(s)	836
AcyI	GRCGYC	3 cut(s)	204, 401, 549
AdeI	CACNNNGTG	1 cut(s)	1557
AfaI	GTAC	2 cut(s)	518, 1149
AfiI	CCNNNNNNNGG	3 cut(s)	696, 1441, 1632
AgsI	TTSAA	3 cut(s)	1399, 1576, 1666
AjnI	CCWGG	3 cut(s)	84, 135, 342
AluBI	AGCT	6 cut(s)	262, 451, 622, 1171, 1279, 1423
AluI	AGCT	6 cut(s)	262, 451, 622, 1171, 1279, 1423
Alw21I	GWGCWC	3 cut(s)	453, 558, 1267
Alw44I	GTGCAC	1 cut(s)	1263
AlwI	GGATC	1 cut(s)	617
AlwNI	CAGNNNCTG	1 cut(s)	917
Ama87I	CYCGRG	2 cut(s)	105, 1358
AoxI	GGCC	6 cut(s)	87, 435, 540, 698, 1044, 1548
ApaLI	GTGCAC	1 cut(s)	1263
ApeKI	GCWGC	3 cut(s)	281, 1312, 1513
ApoI	RAATTY	1 cut(s)	1628
ArsI	GACNNNNNNTTYG	2 cut(s)	240, 272
AseI	ATTAAT	1 cut(s)	1464
AspLEI	GCGC	7 cut(s)	33, 69, 206, 326, 1357, 1441, 1681
AspS9I	GGNCC	8 cut(s)	140, 229, 346, 568, 592, 698, 1044, 1137
AsuC2I	CCSGG	4 cut(s)	572, 1043, 1393, 1459
AsuHPI	GGTGA	9 cut(s)	88, 184, 520, 664, 970, 988, 1103, 1231, 1243
AsuII	TTCGAA	1 cut(s)	127
AvaI	CYCGRG	2 cut(s)	105, 1358
AvaII	GGWCC	6 cut(s)	140, 229, 346, 568, 592, 1137
AxyI	CCTNAGG	1 cut(s)	596
BaeGI	GKGCMC	1 cut(s)	1267
BalI	TGGCCA	1 cut(s)	1550
BanI	GGYRCC	1 cut(s)	203
BanII	GRGCYC	1 cut(s)	453
Bbv12I	GWGCWC	3 cut(s)	453, 558, 1267
BbvCI	CCTCAGC	2 cut(s)	1350, 1424
BbvI	GCAGC	3 cut(s)	268, 1299, 1525
BccI	CCATC	9 cut(s)	217, 287, 532, 643, 703, 796, 1088, 1315, 1547
BceAI	ACGGC	8 cut(s)	95, 107, 193, 217, 376, 422, 527, 536
BcgI	CGANNNNNNTGC	2 cut(s)	471, 505
BciT130I	CCWGG	3 cut(s)	86, 137, 344
BclI	TGATCA	2 cut(s)	846, 960
BcnI	CCSGG	4 cut(s)	572, 1043, 1393, 1459
BfaI	CTAG	2 cut(s)	1178, 1686
BfmI	CTRYAG	1 cut(s)	1313
BfoI	RGCGCY	3 cut(s)	207, 327, 1358
BfuAI	ACCTGC	1 cut(s)	1244
BglII	AGATCT	1 cut(s)	951
BisI	GCNGC	8 cut(s)	102, 243, 282, 285, 690, 1159, 1313, 1514
BlsI	GCNGC	8 cut(s)	103, 244, 283, 286, 691, 1160, 1314, 1515
BmcAI	AGTACT	1 cut(s)	518
Bme1390I	CCNGG	7 cut(s)	86, 137, 344, 572, 1043, 1393, 1459
Bme18I	GGWCC	6 cut(s)	140, 229, 346, 568, 592, 1137
BmeT110I	CYCGRG	2 cut(s)	105, 1358
BmgT120I	GGNCC	8 cut(s)	140, 229, 346, 568, 592, 698, 1044, 1137
BmiI	GGNNCC	6 cut(s)	141, 142, 205, 298, 570, 1196
BmrFI	CCNGG	7 cut(s)	86, 137, 344, 572, 1043, 1393, 1459
BmrI	ACTGGG	1 cut(s)	1064
BmsI	GCATC	7 cut(s)	109, 268, 372, 860, 898, 1195, 1735
BmuI	ACTGGG	1 cut(s)	1064
Bpu10I	CCTNAGC	3 cut(s)	1305, 1350, 1424
Bpu14I	TTCGAA	1 cut(s)	127
BpuMI	CCSGG	4 cut(s)	572, 1043, 1393, 1459
BsaHI	GRCGYC	3 cut(s)	204, 401, 549
BsaJI	CCNNGG	5 cut(s)	136, 144, 432, 543, 561
BsaXI	ACNNNNNCTCC	2 cut(s)	618, 648
Bsc4I	CCNNNNNNNGG	3 cut(s)	696, 1441, 1632
Bse118I	RCCGGY	3 cut(s)	10, 320, 692
Bse1I	ACTGG	5 cut(s)	514, 871, 1059, 1155, 1383
Bse21I	CCTNAGG	1 cut(s)	596
Bse3DI	GCAATG	3 cut(s)	315, 1020, 1517
BseBI	CCWGG	3 cut(s)	86, 137, 344
BseDI	CCNNGG	5 cut(s)	136, 144, 432, 543, 561
BseGI	GGATG	9 cut(s)	78, 283, 363, 714, 875, 973, 1326, 1653, 1698
BseLI	CCNNNNNNNGG	3 cut(s)	696, 1441, 1632
BseMI	GCAATG	3 cut(s)	315, 1020, 1517
BseMII	CTCAG	6 cut(s)	636, 812, 893, 1341, 1410, 1415
BseNI	ACTGG	5 cut(s)	514, 871, 1059, 1155, 1383
BseRI	GAGGAG	3 cut(s)	25, 1091, 1514
BseSI	GKGCMC	1 cut(s)	1267
BseXI	GCAGC	3 cut(s)	268, 1299, 1525
BsgI	GTGCAG	2 cut(s)	1184, 1532
Bsh1236I	CGCG	3 cut(s)	31, 1158, 1439
BshFI	GGCC	6 cut(s)	89, 437, 542, 700, 1046, 1550
BshNI	GGYRCC	1 cut(s)	203
BsiHKAI	GWGCWC	3 cut(s)	453, 558, 1267
BsiHKCI	CYCGRG	2 cut(s)	105, 1358
BsiSI	CCGG	9 cut(s)	11, 288, 321, 572, 581, 693, 1042, 1392, 1458
BslFI	GGGAC	3 cut(s)	153, 425, 672
BslI	CCNNNNNNNGG	3 cut(s)	696, 1441, 1632
BsmFI	GGGAC	3 cut(s)	153, 425, 672
BsmI	GAATGC	2 cut(s)	721, 1393
BsnI	GGCC	6 cut(s)	89, 437, 542, 700, 1046, 1550
BsoBI	CYCGRG	2 cut(s)	105, 1358
Bsp119I	TTCGAA	1 cut(s)	127
Bsp1286I	GDGCHC	3 cut(s)	453, 558, 1267
Bsp19I	CCATGG	1 cut(s)	432
BspANI	GGCC	6 cut(s)	89, 437, 542, 700, 1046, 1550
BspCNI	CTCAG	6 cut(s)	637, 811, 894, 1342, 1411, 1416
BspFNI	CGCG	3 cut(s)	31, 1158, 1439
BspLI	GGNNCC	6 cut(s)	141, 142, 205, 298, 570, 1196
BspMAI	CTGCAG	1 cut(s)	1317
BspMI	ACCTGC	1 cut(s)	1244
BspPI	GGATC	1 cut(s)	617
BspT104I	TTCGAA	1 cut(s)	127
BspT107I	GGYRCC	1 cut(s)	203
BsrBI	CCGCTC	2 cut(s)	104, 1192
BsrDI	GCAATG	3 cut(s)	315, 1020, 1517
BsrFI	RCCGGY	3 cut(s)	10, 320, 692
BsrI	ACTGG	5 cut(s)	514, 871, 1059, 1155, 1383
BssAI	RCCGGY	3 cut(s)	10, 320, 692
BssECI	CCNNGG	5 cut(s)	136, 144, 432, 543, 561
BssNI	GRCGYC	3 cut(s)	204, 401, 549
BssT1I	CCWWGG	2 cut(s)	144, 432
Bst2UI	CCWGG	3 cut(s)	86, 137, 344
Bst4CI	ACNGT	6 cut(s)	307, 339, 376, 461, 634, 1147
Bst6I	CTCTTC	2 cut(s)	816, 887
BstACI	GRCGYC	3 cut(s)	204, 401, 549
BstAPI	GCANNNNNTGC	2 cut(s)	1388, 1519
BstBI	TTCGAA	1 cut(s)	127
BstC8I	GCNNGC	6 cut(s)	322, 484, 531, 1199, 1562, 1683
BstDEI	CTNAG	9 cut(s)	267, 596, 645, 798, 902, 1305, 1350, 1419, 1424
BstDSI	CCRYGG	3 cut(s)	432, 543, 561
BstF5I	GGATG	9 cut(s)	78, 283, 363, 714, 875, 973, 1326, 1653, 1698
BstFNI	CGCG	3 cut(s)	31, 1158, 1439
BstH2I	RGCGCY	3 cut(s)	207, 327, 1358
BstHHI	GCGC	7 cut(s)	33, 69, 206, 326, 1357, 1441, 1681
BstNI	CCWGG	3 cut(s)	86, 137, 344
BstSCI	CCNGG	7 cut(s)	84, 135, 342, 570, 1041, 1391, 1457
BstSFI	CTRYAG	1 cut(s)	1313
BstSLI	GKGCMC	1 cut(s)	1267
BstUI	CGCG	3 cut(s)	31, 1158, 1439
BstV1I	GCAGC	3 cut(s)	268, 1299, 1525
BstX2I	RGATCY	1 cut(s)	951
BstXI	CCANNNNNNTGG	4 cut(s)	151, 1150, 1495, 1546
BstYI	RGATCY	1 cut(s)	951
Bsu36I	CCTNAGG	1 cut(s)	596
BsuRI	GGCC	6 cut(s)	89, 437, 542, 700, 1046, 1550
BtgI	CCRYGG	3 cut(s)	432, 543, 561
BtgZI	GCGATG	1 cut(s)	474
BtsCI	GGATG	9 cut(s)	78, 283, 363, 714, 875, 973, 1326, 1653, 1698
BtsI	GCAGTG	1 cut(s)	21
BtsIMutI	CAGTG	3 cut(s)	21, 344, 1376
BveI	ACCTGC	1 cut(s)	1244
Cac8I	GCNNGC	6 cut(s)	322, 484, 531, 1199, 1562, 1683
CaiI	CAGNNNCTG	1 cut(s)	917
CfoI	GCGC	7 cut(s)	33, 69, 206, 326, 1357, 1441, 1681
Cfr10I	RCCGGY	3 cut(s)	10, 320, 692
Cfr13I	GGNCC	8 cut(s)	140, 229, 346, 568, 592, 698, 1044, 1137
CseI	GACGC	2 cut(s)	390, 557
Csp6I	GTAC	2 cut(s)	517, 1148
CviAII	CATG	2 cut(s)	433, 1129
CviQI	GTAC	2 cut(s)	517, 1148
DdeI	CTNAG	9 cut(s)	267, 596, 645, 798, 902, 1305, 1350, 1419, 1424
DinI	GGCGCC	1 cut(s)	205
DraIII	CACNNNGTG	1 cut(s)	1557
EaeI	YGGCCR	3 cut(s)	435, 540, 1548
Eam1104I	CTCTTC	2 cut(s)	816, 887
EarI	CTCTTC	2 cut(s)	816, 887
EciI	GGCGGA	3 cut(s)	71, 1202, 1705
Ecl136II	GAGCTC	1 cut(s)	451
Eco130I	CCWWGG	2 cut(s)	144, 432
Eco24I	GRGCYC	1 cut(s)	453
Eco32I	GATATC	1 cut(s)	604
Eco47I	GGWCC	6 cut(s)	140, 229, 346, 568, 592, 1137
Eco53kI	GAGCTC	1 cut(s)	451
Eco57I	CTGAAG	1 cut(s)	836
Eco81I	CCTNAGG	1 cut(s)	596
Eco88I	CYCGRG	2 cut(s)	105, 1358
EcoICRI	GAGCTC	1 cut(s)	451
EcoO109I	RGGNCCY	3 cut(s)	140, 229, 346
EcoRII	CCWGG	3 cut(s)	84, 135, 342
EcoRV	GATATC	1 cut(s)	604
EcoT14I	CCWWGG	2 cut(s)	144, 432
EcoT22I	ATGCAT	1 cut(s)	1728
EcoT38I	GRGCYC	1 cut(s)	453
EgeI	GGCGCC	1 cut(s)	205
EheI	GGCGCC	1 cut(s)	205
ErhI	CCWWGG	2 cut(s)	144, 432
FaeI	CATG	2 cut(s)	436, 1132
FaqI	GGGAC	3 cut(s)	153, 425, 672
FatI	CATG	2 cut(s)	432, 1128
FauI	CCCGC	1 cut(s)	247
FbaI	TGATCA	2 cut(s)	846, 960
FblI	GTMKAC	3 cut(s)	57, 417, 636
Fnu4HI	GCNGC	8 cut(s)	102, 243, 282, 285, 690, 1159, 1313, 1514
FokI	GGATG	9 cut(s)	65, 290, 350, 721, 882, 980, 1333, 1660, 1705
FriOI	GRGCYC	1 cut(s)	453
Fsp4HI	GCNGC	8 cut(s)	102, 243, 282, 285, 690, 1159, 1313, 1514
FspBI	CTAG	2 cut(s)	1178, 1686
GlaI	GCGC	7 cut(s)	32, 68, 205, 325, 1356, 1440, 1680
GluI	GCNGC	8 cut(s)	102, 243, 282, 285, 690, 1159, 1313, 1514
HaeII	RGCGCY	3 cut(s)	207, 327, 1358
HaeIII	GGCC	6 cut(s)	89, 437, 542, 700, 1046, 1550
HapII	CCGG	9 cut(s)	11, 288, 321, 572, 581, 693, 1042, 1392, 1458
HgaI	GACGC	2 cut(s)	390, 557
HhaI	GCGC	7 cut(s)	33, 69, 206, 326, 1357, 1441, 1681
Hin1I	GRCGYC	3 cut(s)	204, 401, 549
Hin1II	CATG	2 cut(s)	436, 1132
Hin6I	GCGC	7 cut(s)	31, 67, 204, 324, 1355, 1439, 1679
HinP1I	GCGC	7 cut(s)	31, 67, 204, 324, 1355, 1439, 1679
HincII	GTYRAC	2 cut(s)	58, 637
HindII	GTYRAC	2 cut(s)	58, 637
HpaII	CCGG	9 cut(s)	11, 288, 321, 572, 581, 693, 1042, 1392, 1458
HphI	GGTGA	9 cut(s)	88, 184, 520, 664, 970, 988, 1103, 1231, 1243
Hpy166II	GTNNAC	9 cut(s)	58, 405, 418, 547, 568, 592, 637, 1265, 1653
Hpy188III	TCNNGA	7 cut(s)	331, 424, 607, 613, 742, 850, 1645
Hpy8I	GTNNAC	9 cut(s)	58, 405, 418, 547, 568, 592, 637, 1265, 1653
Hpy99I	CGWCG	6 cut(s)	203, 260, 311, 314, 641, 706
HpyAV	CCTTC	5 cut(s)	359, 649, 670, 811, 1468
HpyCH4III	ACNGT	6 cut(s)	307, 339, 376, 461, 634, 1147
HpyCH4IV	ACGT	1 cut(s)	876
HpyF3I	CTNAG	9 cut(s)	267, 596, 645, 798, 902, 1305, 1350, 1419, 1424
HpySE526I	ACGT	1 cut(s)	876
Hsp92I	GRCGYC	3 cut(s)	204, 401, 549
Hsp92II	CATG	2 cut(s)	436, 1132
HspAI	GCGC	7 cut(s)	31, 67, 204, 324, 1355, 1439, 1679
KasI	GGCGCC	1 cut(s)	203
KflI	GGGWCCC	1 cut(s)	140
KroI	GCCGGC	1 cut(s)	320
KroNI	GCCGGC	1 cut(s)	322
Ksp22I	TGATCA	2 cut(s)	846, 960
LmnI	GCTCC	3 cut(s)	38, 1189, 1194
Lsp1109I	GCAGC	3 cut(s)	268, 1299, 1525
LweI	GCATC	7 cut(s)	109, 268, 372, 860, 898, 1195, 1735
MaeI	CTAG	2 cut(s)	1178, 1686
MaeII	ACGT	1 cut(s)	876
MaeIII	GTNAC	7 cut(s)	270, 354, 683, 913, 1414, 1591, 1700
MbiI	CCGCTC	2 cut(s)	104, 1192
MboII	GAAGA	8 cut(s)	628, 833, 865, 904, 961, 1111, 1120, 1588
MflI	RGATCY	1 cut(s)	951
MhlI	GDGCHC	3 cut(s)	453, 558, 1267
MlsI	TGGCCA	1 cut(s)	1550
MluCI	AATT	5 cut(s)	829, 1009, 1404, 1628, 1731
MluNI	TGGCCA	1 cut(s)	1550
Mly113I	GGCGCC	1 cut(s)	204
MlyI	GAGTC	5 cut(s)	56, 380, 517, 691, 997
MmeI	TCCRAC	2 cut(s)	259, 1115
Mox20I	TGGCCA	1 cut(s)	1550
Mph1103I	ATGCAT	1 cut(s)	1728
MroNI	GCCGGC	1 cut(s)	320
MscI	TGGCCA	1 cut(s)	1550
MseI	TTAA	3 cut(s)	216, 1464, 1747
MslI	CAYNNNNRTG	1 cut(s)	1721
Msp20I	TGGCCA	1 cut(s)	1550
MspA1I	CMGCKG	1 cut(s)	1171
MspI	CCGG	9 cut(s)	11, 288, 321, 572, 581, 693, 1042, 1392, 1458
MspR9I	CCNGG	7 cut(s)	86, 137, 344, 572, 1043, 1393, 1459
Mva1269I	GAATGC	2 cut(s)	721, 1393
MvaI	CCWGG	3 cut(s)	86, 137, 344
MvnI	CGCG	3 cut(s)	31, 1158, 1439
NaeI	GCCGGC	1 cut(s)	322
NarI	GGCGCC	1 cut(s)	204
NciI	CCSGG	4 cut(s)	572, 1043, 1393, 1459
NcoI	CCATGG	1 cut(s)	432
NgoMIV	GCCGGC	1 cut(s)	320
NlaIII	CATG	2 cut(s)	436, 1132
NlaIV	GGNNCC	6 cut(s)	141, 142, 205, 298, 570, 1196
NmeAIII	GCCGAG	1 cut(s)	1072
NmuCI	GTSAC	5 cut(s)	270, 354, 683, 1414, 1591
NsiI	ATGCAT	1 cut(s)	1728
NspV	TTCGAA	1 cut(s)	127
PaeR7I	CTCGAG	2 cut(s)	105, 1358
PaqCI	CACCTGC	1 cut(s)	1244
PctI	GAATGC	2 cut(s)	721, 1393
PdiI	GCCGGC	1 cut(s)	322
PfeI	GAWTC	8 cut(s)	211, 464, 733, 811, 886, 1120, 1165, 1429
PflFI	GACNNNGTC	2 cut(s)	46, 1589
PkrI	GCNGC	8 cut(s)	103, 244, 283, 286, 691, 1160, 1314, 1515
PleI	GAGTC	5 cut(s)	55, 380, 517, 690, 997
PluTI	GGCGCC	1 cut(s)	207
PpsI	GAGTC	5 cut(s)	55, 380, 517, 690, 997
PpuMI	RGGWCCY	3 cut(s)	140, 229, 346
PshBI	ATTAAT	1 cut(s)	1464
Psp124BI	GAGCTC	1 cut(s)	453
Psp1406I	AACGTT	1 cut(s)	876
Psp5II	RGGWCCY	3 cut(s)	140, 229, 346
Psp6I	CCWGG	3 cut(s)	84, 135, 342
PspGI	CCWGG	3 cut(s)	84, 135, 342
PspN4I	GGNNCC	6 cut(s)	141, 142, 205, 298, 570, 1196
PspPI	GGNCC	8 cut(s)	140, 229, 346, 568, 592, 698, 1044, 1137
PspPPI	RGGWCCY	3 cut(s)	140, 229, 346
PspXI	VCTCGAGB	2 cut(s)	105, 1358
PstI	CTGCAG	1 cut(s)	1317
PstNI	CAGNNNCTG	1 cut(s)	917
PsuI	RGATCY	1 cut(s)	951
PsyI	GACNNNGTC	2 cut(s)	46, 1589
PvuII	CAGCTG	1 cut(s)	1171
RsaI	GTAC	2 cut(s)	518, 1149
RsaNI	GTAC	2 cut(s)	517, 1148
RseI	CAYNNNNRTG	1 cut(s)	1721
SacI	GAGCTC	1 cut(s)	453
SalI	GTCGAC	2 cut(s)	56, 635
SaqAI	TTAA	3 cut(s)	216, 1464, 1747
SatI	GCNGC	8 cut(s)	102, 243, 282, 285, 690, 1159, 1313, 1514
Sau96I	GGNCC	8 cut(s)	140, 229, 346, 568, 592, 698, 1044, 1137
ScaI	AGTACT	1 cut(s)	518
SchI	GAGTC	5 cut(s)	56, 380, 517, 691, 997
ScrFI	CCNGG	7 cut(s)	86, 137, 344, 572, 1043, 1393, 1459
SduI	GDGCHC	3 cut(s)	453, 558, 1267
SfaNI	GCATC	7 cut(s)	109, 268, 372, 860, 898, 1195, 1735
SfcI	CTRYAG	1 cut(s)	1313
SfoI	GGCGCC	1 cut(s)	205
Sfr274I	CTCGAG	2 cut(s)	105, 1358
SfuI	TTCGAA	1 cut(s)	127
SgrAI	CRCCGGYG	1 cut(s)	692
SinI	GGWCC	6 cut(s)	140, 229, 346, 568, 592, 1137
SlaI	CTCGAG	2 cut(s)	105, 1358
SmiMI	CAYNNNNRTG	1 cut(s)	1721
SmlI	CTYRAG	2 cut(s)	105, 1358
SmoI	CTYRAG	2 cut(s)	105, 1358
Sse9I	AATT	5 cut(s)	829, 1009, 1404, 1628, 1731
SspDI	GGCGCC	1 cut(s)	203
SspMI	CTAG	2 cut(s)	1178, 1686
SstI	GAGCTC	1 cut(s)	453
StyD4I	CCNGG	7 cut(s)	84, 135, 342, 570, 1041, 1391, 1457
StyI	CCWWGG	2 cut(s)	144, 432
TaaI	ACNGT	6 cut(s)	307, 339, 376, 461, 634, 1147
TaiI	ACGT	1 cut(s)	879
TaqII	GACCGA	1 cut(s)	59
TasI	AATT	5 cut(s)	829, 1009, 1404, 1628, 1731
TatI	WGTACW	1 cut(s)	516
TauI	GCSGC	5 cut(s)	104, 245, 287, 692, 1161
TfiI	GAWTC	8 cut(s)	211, 464, 733, 811, 886, 1120, 1165, 1429
Tru1I	TTAA	3 cut(s)	216, 1464, 1747
Tru9I	TTAA	3 cut(s)	216, 1464, 1747
TscAI	CASTG	3 cut(s)	21, 344, 1383
TseFI	GTSAC	5 cut(s)	270, 354, 683, 1414, 1591
TseI	GCWGC	3 cut(s)	281, 1312, 1513
Tsp45I	GTSAC	5 cut(s)	270, 354, 683, 1414, 1591
TspDTI	ATGAA	2 cut(s)	982, 1593
TspGWI	ACGGA	1 cut(s)	550
TspRI	CASTG	3 cut(s)	21, 344, 1383
Tth111I	GACNNNGTC	2 cut(s)	46, 1589
VneI	GTGCAC	1 cut(s)	1263
VpaK11BI	GGWCC	6 cut(s)	140, 229, 346, 568, 592, 1137
VspI	ATTAAT	1 cut(s)	1464
XapI	RAATTY	1 cut(s)	1628
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1147
XhoI	CTCGAG	2 cut(s)	105, 1358
XmiI	GTMKAC	3 cut(s)	57, 417, 636
XspI	CTAG	2 cut(s)	1178, 1686
ZrmI	AGTACT	1 cut(s)	518
Zsp2I	ATGCAT	1 cut(s)	1728

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.