Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0016221

Physical Location & Seq

Genomic Coordinates Reverse (-)

20662870 .. 20664672

Gene Structure 1803 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom14g19270.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1671 bp

ATGGCTTCACCGGCAGTGATCTCCCAGCCGCGCTCCTCCAAGACCGAGTCCTATGTCGACAACAAGCGCAAGGAGGACATCCGCCAGGCCAACATCACCGCCGCTCGAGCCGTCGCCGATGCCGTCCGAACCAGCCTGGGGCCCAAGGGCATGGACAAGATGATCTCCACCGCTAACGGCGAGGTTATCATCACCAACGATGGCGCCACGATTCTTAACAAGATGGAGGTCCTCCAACCCGCCGCCAAGATGCTTGTCGAGCTTTCTAAGTCCCAGGATGCTGCCGCCGGAGATGGAACCACCACCGTCGTCGTCATTGCTGGCGCTCTCCTGAAACAGTGCCAGGTCCTTCTGTCACACGGCATCCACCCGACTGTGATCTCTGACTCTCTCCACAAGGCGTCCACCAAAGCGGTAGACGTCTTGACCGCCATGGCCGTCCCCGTTGAGCTCACAGACCGTGATTCGCTGGTAAAGTCTGCAAGCACATCGCTCAACAGCAAGGTGGTGAGCCAGTACTCGACTCTGCTCGCGCCGATGGCCGTGGACGCCGTGCTCTCCGTGGTGGACCCGGCGAAGCCGGAGATAGTGGACCTTAGGGACATCAGGATCGTGAAGAAGCTCGGAGGAACTGTCGACGATACTGAGATGGTGAAGGGACTTGTTTTCGATAAGAAGGCGAGTCACGCCGCCGGTGGGCCGACGAGGATGGAGAATGCCAAGATTGCTGTGATTCAGTTCCAGATATCGCCTCCGAAAACCGATATCGAGCAGAGCATTGTCGTCTCGGATTATACTCAGATGGATAGGATTCTGAAGGAAGAGAGAAATTACATTCTGGGTATGATAAAAAAGATCAAGGCTACCGGGTGCAACGTTTTGCTGATTCAGAAGAGTATTCTGAGAGATGCAGTTACTGATTTGTCTTTGCATTACTTGGCGAAAGCGAAGATCTTGGTGATCAAAGATGTGGAGAGAGATGAGATTGAGTTCATCACCAAGACTCTGAATTGCTTGCCCATTGCGAATATCGAGCATTTCCGGGCCGAGAAACTGGGGTATGCTGATATGATCACAGGGATTCAGAACATGGGTCGGACCACCACGGTGCTGGTTCGCGGCTCGAATCAACTGGTGCTAGATGAGGCGGAGCGGAGCCTGCACGATGCTCTTTGTGTGGTGAGGTGTTTGGTGAGCAAGAGGTTTTTGATTGCAGGTGGTGGTGCACCAGAGATTGAGCTGTCGAGGCAGTTGGGGGCGTGGGCTAAGGTGCTGCAGGGGATGGAGGGTTATTGTGTGAAGTCGTTTGCTGAGGCGCTCGAGGTTGTTCCCTATACACTGGCTGAGAATGCCGGGTTGAACCCAATTTCGATTGTGACTGAGCTGAGGAATCGCCACGGGCAGATTACCAACATCTTGGAGGAGAATGTGGTGCAGCCATTGCTCGTCAGCACAAGTGCCATCACTCTGGCCACCGAGTGCGTGCGGATGATTTTGAAGATTGATGACATAGTTACTTCTATGTCATTCGAGATGGATTTCTGTTCCGAATTTTGGTACTGGTTCTGGATGTTTACTTTGGATTTGAACTTTGTTTTTGTGTTTGTTAGGCGGATGTTGTGTAACCTACCTTTGCCCATTTATATGCATCTAATTAGTTTGTCGTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005832 GO:0006457 GO:0006458 GO:0008150 GO:0009987 GO:0010035 GO:0010038 GO:0032991 GO:0042221 GO:0044183 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0046686 GO:0050896 GO:0051082 GO:0061077 GO:0101031

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cpn60_TCP1

Protein Analysis

557

Amino Acids

60.97

Weight (kDa)

6.35

Isoelectric Point (pI)

39.79

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18190
fragaria_vesca	FvH4_7g06380
malus_domestica	MD06G1222400.v1.1 MD14G1231500.v1.1
prunus_persica	Prupe.5G227000_v2.0.a1
pyrus_communis	pycom14g19270
rosa_chinensis	RchiOBHm_Chr1g0334481
rosa_laevigata	RLG00000029450
rosa_multiflora	Rmu_ssc0000404.1_g000012
rosa_roxburghii	Rroxscaffold_4G00316810
rosa_rugosa	Rorug01G0114200
rosa_samantha	Rh1AG139400 Rh1BG105800 Rh1DG143300
rosa_wichuraiana	Rw1G011490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1205
AatII	GACGTC	1 cut(s)	423
Acc36I	ACCTGC	1 cut(s)	1205
AccB1I	GGYRCC	1 cut(s)	203
AccBSI	CCGCTC	2 cut(s)	104, 1153
AccI	GTMKAC	3 cut(s)	57, 417, 636
AccII	CGCG	3 cut(s)	31, 533, 1119
AclI	AACGTT	1 cut(s)	876
AclWI	GGATC	1 cut(s)	617
AcoI	YGGCCR	3 cut(s)	435, 540, 1470
AcsI	RAATTY	1 cut(s)	1550
AcuI	CTGAAG	1 cut(s)	836
AcyI	GRCGYC	4 cut(s)	204, 401, 420, 549
AdeI	CACNNNGTG	1 cut(s)	1479
AfaI	GTAC	2 cut(s)	518, 1559
AfiI	CCNNNNNNNGG	3 cut(s)	138, 696, 1554
AgsI	TTSAA	3 cut(s)	1360, 1498, 1588
AjnI	CCWGG	4 cut(s)	84, 135, 273, 342
AleI	CACNNNNGTG	1 cut(s)	1106
AluBI	AGCT	5 cut(s)	262, 451, 622, 1240, 1384
AluI	AGCT	5 cut(s)	262, 451, 622, 1240, 1384
Alw21I	GWGCWC	3 cut(s)	453, 558, 1228
Alw26I	GTCTC	1 cut(s)	790
Alw44I	GTGCAC	1 cut(s)	1224
AlwI	GGATC	1 cut(s)	617
AlwNI	CAGNNNCTG	1 cut(s)	917
Ama87I	CYCGRG	2 cut(s)	105, 1319
AoxI	GGCC	7 cut(s)	87, 140, 435, 540, 698, 1044, 1470
ApaI	GGGCCC	1 cut(s)	144
ApaLI	GTGCAC	1 cut(s)	1224
ApeKI	GCWGC	3 cut(s)	281, 1273, 1435
ApoI	RAATTY	1 cut(s)	1550
AspLEI	GCGC	6 cut(s)	33, 69, 206, 326, 535, 1318
AspS9I	GGNCC	9 cut(s)	140, 141, 229, 346, 568, 592, 698, 1044, 1098
AsuC2I	CCSGG	4 cut(s)	572, 868, 1043, 1354
AsuHPI	GGTGA	8 cut(s)	88, 184, 520, 664, 970, 988, 1192, 1204
AvaI	CYCGRG	2 cut(s)	105, 1319
AvaII	GGWCC	5 cut(s)	229, 346, 568, 592, 1098
AxyI	CCTNAGG	1 cut(s)	596
BaeGI	GKGCMC	2 cut(s)	144, 1228
BalI	TGGCCA	1 cut(s)	1472
BanI	GGYRCC	1 cut(s)	203
BanII	GRGCYC	2 cut(s)	144, 453
Bbv12I	GWGCWC	3 cut(s)	453, 558, 1228
BbvCI	CCTCAGC	2 cut(s)	1311, 1385
BbvI	GCAGC	3 cut(s)	268, 1260, 1447
BceAI	ACGGC	7 cut(s)	95, 107, 193, 376, 422, 527, 536
BcgI	CGANNNNNNTGC	2 cut(s)	471, 505
BciT130I	CCWGG	4 cut(s)	86, 137, 275, 344
BclI	TGATCA	2 cut(s)	960, 1071
BcnI	CCSGG	4 cut(s)	572, 868, 1043, 1354
BcoDI	GTCTC	1 cut(s)	790
BfaI	CTAG	1 cut(s)	1139
BfmI	CTRYAG	1 cut(s)	1274
BfoI	RGCGCY	3 cut(s)	207, 327, 1319
BfuAI	ACCTGC	1 cut(s)	1205
BglII	AGATCT	1 cut(s)	951
BisI	GCNGC	9 cut(s)	29, 102, 243, 282, 285, 690, 1120, 1274, 1436
BlsI	GCNGC	9 cut(s)	30, 103, 244, 283, 286, 691, 1121, 1275, 1437
BmcAI	AGTACT	1 cut(s)	518
Bme1390I	CCNGG	8 cut(s)	86, 137, 275, 344, 572, 868, 1043, 1354
Bme18I	GGWCC	5 cut(s)	229, 346, 568, 592, 1098
BmeT110I	CYCGRG	2 cut(s)	105, 1319
BmgT120I	GGNCC	9 cut(s)	140, 141, 229, 346, 568, 592, 698, 1044, 1098
BmiI	GGNNCC	6 cut(s)	141, 142, 205, 298, 570, 1157
BmrFI	CCNGG	8 cut(s)	86, 137, 275, 344, 572, 868, 1043, 1354
BmrI	ACTGGG	1 cut(s)	1064
BmsI	GCATC	7 cut(s)	109, 240, 268, 372, 898, 1156, 1657
BmuI	ACTGGG	1 cut(s)	1064
Bpu10I	CCTNAGC	3 cut(s)	1266, 1311, 1385
BpuMI	CCSGG	4 cut(s)	572, 868, 1043, 1354
BsaHI	GRCGYC	4 cut(s)	204, 401, 420, 549
BsaJI	CCNNGG	8 cut(s)	136, 144, 273, 432, 543, 561, 1104, 1396
BsaXI	ACNNNNNCTCC	2 cut(s)	618, 648
Bsc4I	CCNNNNNNNGG	3 cut(s)	138, 696, 1554
Bse118I	RCCGGY	2 cut(s)	10, 692
Bse1I	ACTGG	5 cut(s)	514, 1059, 1137, 1344, 1565
Bse21I	CCTNAGG	1 cut(s)	596
Bse3DI	GCAATG	3 cut(s)	315, 1020, 1439
BseBI	CCWGG	4 cut(s)	86, 137, 275, 344
BseDI	CCNNGG	8 cut(s)	136, 144, 273, 432, 543, 561, 1104, 1396
BseGI	GGATG	8 cut(s)	78, 283, 363, 714, 1287, 1494, 1575, 1620
BseLI	CCNNNNNNNGG	3 cut(s)	138, 696, 1554
BseMI	GCAATG	3 cut(s)	315, 1020, 1439
BseMII	CTCAG	7 cut(s)	636, 812, 893, 1302, 1335, 1371, 1376
BseNI	ACTGG	5 cut(s)	514, 1059, 1137, 1344, 1565
BseRI	GAGGAG	2 cut(s)	25, 1436
BseSI	GKGCMC	2 cut(s)	144, 1228
BseXI	GCAGC	3 cut(s)	268, 1260, 1447
BseYI	CCCAGC	1 cut(s)	24
BsgI	GTGCAG	2 cut(s)	1145, 1454
Bsh1236I	CGCG	3 cut(s)	31, 533, 1119
BshFI	GGCC	7 cut(s)	89, 142, 437, 542, 700, 1046, 1472
BshNI	GGYRCC	1 cut(s)	203
BsiHKAI	GWGCWC	3 cut(s)	453, 558, 1228
BsiHKCI	CYCGRG	2 cut(s)	105, 1319
BsiSI	CCGG	8 cut(s)	11, 288, 572, 581, 693, 867, 1042, 1353
BslFI	GGGAC	4 cut(s)	256, 425, 614, 672
BslI	CCNNNNNNNGG	3 cut(s)	138, 696, 1554
BsmAI	GTCTC	1 cut(s)	790
BsmBI	CGTCTC	1 cut(s)	790
BsmFI	GGGAC	4 cut(s)	256, 425, 614, 672
BsmI	GAATGC	2 cut(s)	721, 1354
BsnI	GGCC	7 cut(s)	89, 142, 437, 542, 700, 1046, 1472
BsoBI	CYCGRG	2 cut(s)	105, 1319
Bsp120I	GGGCCC	1 cut(s)	140
Bsp1286I	GDGCHC	4 cut(s)	144, 453, 558, 1228
Bsp143I	GATC	8 cut(s)	18, 162, 378, 609, 855, 951, 960, 1071
Bsp19I	CCATGG	1 cut(s)	432
BspANI	GGCC	7 cut(s)	89, 142, 437, 542, 700, 1046, 1472
BspCNI	CTCAG	7 cut(s)	637, 811, 894, 1303, 1336, 1372, 1377
BspFNI	CGCG	3 cut(s)	31, 533, 1119
BspLI	GGNNCC	6 cut(s)	141, 142, 205, 298, 570, 1157
BspMAI	CTGCAG	1 cut(s)	1278
BspMI	ACCTGC	1 cut(s)	1205
BspPI	GGATC	1 cut(s)	617
BspT107I	GGYRCC	1 cut(s)	203
BsrBI	CCGCTC	2 cut(s)	104, 1153
BsrDI	GCAATG	3 cut(s)	315, 1020, 1439
BsrFI	RCCGGY	2 cut(s)	10, 692
BsrI	ACTGG	5 cut(s)	514, 1059, 1137, 1344, 1565
BssAI	RCCGGY	2 cut(s)	10, 692
BssECI	CCNNGG	8 cut(s)	136, 144, 273, 432, 543, 561, 1104, 1396
BssMI	GATC	8 cut(s)	18, 162, 378, 609, 855, 951, 960, 1071
BssNI	GRCGYC	4 cut(s)	204, 401, 420, 549
BssT1I	CCWWGG	2 cut(s)	144, 432
Bst2UI	CCWGG	4 cut(s)	86, 137, 275, 344
Bst4CI	ACNGT	6 cut(s)	307, 339, 376, 461, 634, 1108
Bst6I	CTCTTC	2 cut(s)	816, 887
BstACI	GRCGYC	4 cut(s)	204, 401, 420, 549
BstAPI	GCANNNNNTGC	1 cut(s)	1441
BstC8I	GCNNGC	6 cut(s)	322, 484, 531, 1016, 1160, 1484
BstDSI	CCRYGG	5 cut(s)	432, 543, 561, 1104, 1396
BstF5I	GGATG	8 cut(s)	78, 283, 363, 714, 1287, 1494, 1575, 1620
BstFNI	CGCG	3 cut(s)	31, 533, 1119
BstH2I	RGCGCY	3 cut(s)	207, 327, 1319
BstHHI	GCGC	6 cut(s)	33, 69, 206, 326, 535, 1318
BstKTI	GATC	8 cut(s)	21, 165, 381, 612, 858, 954, 963, 1074
BstMAI	GTCTC	1 cut(s)	790
BstMBI	GATC	8 cut(s)	18, 162, 378, 609, 855, 951, 960, 1071
BstNI	CCWGG	4 cut(s)	86, 137, 275, 344
BstSCI	CCNGG	8 cut(s)	84, 135, 273, 342, 570, 866, 1041, 1352
BstSFI	CTRYAG	1 cut(s)	1274
BstSLI	GKGCMC	2 cut(s)	144, 1228
BstUI	CGCG	3 cut(s)	31, 533, 1119
BstV1I	GCAGC	3 cut(s)	268, 1260, 1447
BstX2I	RGATCY	1 cut(s)	951
BstXI	CCANNNNNNTGG	4 cut(s)	151, 1111, 1417, 1468
BstYI	RGATCY	1 cut(s)	951
Bsu36I	CCTNAGG	1 cut(s)	596
BsuRI	GGCC	7 cut(s)	89, 142, 437, 542, 700, 1046, 1472
BtgI	CCRYGG	5 cut(s)	432, 543, 561, 1104, 1396
BtgZI	GCGATG	1 cut(s)	474
BtsCI	GGATG	8 cut(s)	78, 283, 363, 714, 1287, 1494, 1575, 1620
BtsI	GCAGTG	1 cut(s)	21
BtsIMutI	CAGTG	3 cut(s)	21, 344, 1337
BveI	ACCTGC	1 cut(s)	1205
Cac8I	GCNNGC	6 cut(s)	322, 484, 531, 1016, 1160, 1484
CaiI	CAGNNNCTG	1 cut(s)	917
CfoI	GCGC	6 cut(s)	33, 69, 206, 326, 535, 1318
Cfr10I	RCCGGY	2 cut(s)	10, 692
Cfr13I	GGNCC	9 cut(s)	140, 141, 229, 346, 568, 592, 698, 1044, 1098
CseI	GACGC	2 cut(s)	390, 557
Csp6I	GTAC	2 cut(s)	517, 1558
CviAII	CATG	3 cut(s)	151, 433, 1090
CviQI	GTAC	2 cut(s)	517, 1558
DinI	GGCGCC	1 cut(s)	205
DpnI	GATC	8 cut(s)	20, 164, 380, 611, 857, 953, 962, 1073
DpnII	GATC	8 cut(s)	18, 162, 378, 609, 855, 951, 960, 1071
DraIII	CACNNNGTG	1 cut(s)	1479
EaeI	YGGCCR	3 cut(s)	435, 540, 1470
Eam1104I	CTCTTC	2 cut(s)	816, 887
EarI	CTCTTC	2 cut(s)	816, 887
EciI	GGCGGA	3 cut(s)	71, 1163, 1627
Ecl136II	GAGCTC	1 cut(s)	451
Eco130I	CCWWGG	2 cut(s)	144, 432
Eco24I	GRGCYC	2 cut(s)	144, 453
Eco32I	GATATC	2 cut(s)	747, 766
Eco47I	GGWCC	5 cut(s)	229, 346, 568, 592, 1098
Eco53kI	GAGCTC	1 cut(s)	451
Eco57I	CTGAAG	1 cut(s)	836
Eco81I	CCTNAGG	1 cut(s)	596
Eco88I	CYCGRG	2 cut(s)	105, 1319
EcoICRI	GAGCTC	1 cut(s)	451
EcoO109I	RGGNCCY	3 cut(s)	140, 229, 346
EcoRII	CCWGG	4 cut(s)	84, 135, 273, 342
EcoRV	GATATC	2 cut(s)	747, 766
EcoT14I	CCWWGG	2 cut(s)	144, 432
EcoT22I	ATGCAT	1 cut(s)	1650
EcoT38I	GRGCYC	2 cut(s)	144, 453
EgeI	GGCGCC	1 cut(s)	205
EheI	GGCGCC	1 cut(s)	205
ErhI	CCWWGG	2 cut(s)	144, 432
Esp3I	CGTCTC	1 cut(s)	790
FaeI	CATG	3 cut(s)	154, 436, 1093
FaqI	GGGAC	4 cut(s)	256, 425, 614, 672
FatI	CATG	3 cut(s)	150, 432, 1089
FauI	CCCGC	1 cut(s)	247
FbaI	TGATCA	2 cut(s)	960, 1071
FblI	GTMKAC	3 cut(s)	57, 417, 636
Fnu4HI	GCNGC	9 cut(s)	29, 102, 243, 282, 285, 690, 1120, 1274, 1436
FokI	GGATG	8 cut(s)	65, 290, 350, 721, 1294, 1501, 1582, 1627
FriOI	GRGCYC	2 cut(s)	144, 453
Fsp4HI	GCNGC	9 cut(s)	29, 102, 243, 282, 285, 690, 1120, 1274, 1436
FspBI	CTAG	1 cut(s)	1139
GlaI	GCGC	6 cut(s)	32, 68, 205, 325, 534, 1317
GluI	GCNGC	9 cut(s)	29, 102, 243, 282, 285, 690, 1120, 1274, 1436
GsaI	CCCAGC	1 cut(s)	28
HaeII	RGCGCY	3 cut(s)	207, 327, 1319
HaeIII	GGCC	7 cut(s)	89, 142, 437, 542, 700, 1046, 1472
HapII	CCGG	8 cut(s)	11, 288, 572, 581, 693, 867, 1042, 1353
HgaI	GACGC	2 cut(s)	390, 557
HhaI	GCGC	6 cut(s)	33, 69, 206, 326, 535, 1318
Hin1I	GRCGYC	4 cut(s)	204, 401, 420, 549
Hin1II	CATG	3 cut(s)	154, 436, 1093
Hin6I	GCGC	6 cut(s)	31, 67, 204, 324, 533, 1316
HinP1I	GCGC	6 cut(s)	31, 67, 204, 324, 533, 1316
HincII	GTYRAC	2 cut(s)	58, 637
HindII	GTYRAC	2 cut(s)	58, 637
HpaII	CCGG	8 cut(s)	11, 288, 572, 581, 693, 867, 1042, 1353
HphI	GGTGA	8 cut(s)	88, 184, 520, 664, 970, 988, 1192, 1204
Hpy166II	GTNNAC	9 cut(s)	58, 405, 418, 547, 568, 592, 637, 1226, 1575
Hpy188III	TCNNGA	7 cut(s)	331, 424, 607, 613, 742, 1531, 1567
Hpy8I	GTNNAC	9 cut(s)	58, 405, 418, 547, 568, 592, 637, 1226, 1575
Hpy99I	CGWCG	5 cut(s)	116, 311, 314, 641, 706
HpyAV	CCTTC	4 cut(s)	359, 649, 670, 811
HpyCH4III	ACNGT	6 cut(s)	307, 339, 376, 461, 634, 1108
HpyCH4IV	ACGT	2 cut(s)	420, 876
HpySE526I	ACGT	2 cut(s)	420, 876
Hsp92I	GRCGYC	4 cut(s)	204, 401, 420, 549
Hsp92II	CATG	3 cut(s)	154, 436, 1093
HspAI	GCGC	6 cut(s)	31, 67, 204, 324, 533, 1316
KasI	GGCGCC	1 cut(s)	203
Ksp22I	TGATCA	2 cut(s)	960, 1071
Kzo9I	GATC	8 cut(s)	18, 162, 378, 609, 855, 951, 960, 1071
LmnI	GCTCC	3 cut(s)	38, 1150, 1155
Lsp1109I	GCAGC	3 cut(s)	268, 1260, 1447
LweI	GCATC	7 cut(s)	109, 240, 268, 372, 898, 1156, 1657
MaeI	CTAG	1 cut(s)	1139
MaeII	ACGT	2 cut(s)	420, 876
MaeIII	GTNAC	6 cut(s)	354, 683, 913, 1375, 1513, 1622
MalI	GATC	8 cut(s)	20, 164, 380, 611, 857, 953, 962, 1073
MbiI	CCGCTC	2 cut(s)	104, 1153
MboI	GATC	8 cut(s)	18, 162, 378, 609, 855, 951, 960, 1071
MboII	GAAGA	5 cut(s)	628, 833, 904, 961, 1510
MflI	RGATCY	1 cut(s)	951
MhlI	GDGCHC	4 cut(s)	144, 453, 558, 1228
MlsI	TGGCCA	1 cut(s)	1472
MluCI	AATT	5 cut(s)	829, 1009, 1365, 1550, 1653
MluNI	TGGCCA	1 cut(s)	1472
Mly113I	GGCGCC	1 cut(s)	204
MlyI	GAGTC	5 cut(s)	56, 380, 517, 691, 997
MmeI	TCCRAC	2 cut(s)	259, 1076
Mox20I	TGGCCA	1 cut(s)	1472
Mph1103I	ATGCAT	1 cut(s)	1650
MscI	TGGCCA	1 cut(s)	1472
MseI	TTAA	2 cut(s)	216, 1669
MslI	CAYNNNNRTG	2 cut(s)	1106, 1643
Msp20I	TGGCCA	1 cut(s)	1472
MspI	CCGG	8 cut(s)	11, 288, 572, 581, 693, 867, 1042, 1353
MspR9I	CCNGG	8 cut(s)	86, 137, 275, 344, 572, 868, 1043, 1354
Mva1269I	GAATGC	2 cut(s)	721, 1354
MvaI	CCWGG	4 cut(s)	86, 137, 275, 344
MvnI	CGCG	3 cut(s)	31, 533, 1119
NarI	GGCGCC	1 cut(s)	204
NciI	CCSGG	4 cut(s)	572, 868, 1043, 1354
NcoI	CCATGG	1 cut(s)	432
NdeII	GATC	8 cut(s)	18, 162, 378, 609, 855, 951, 960, 1071
NlaIII	CATG	3 cut(s)	154, 436, 1093
NlaIV	GGNNCC	6 cut(s)	141, 142, 205, 298, 570, 1157
NmeAIII	GCCGAG	1 cut(s)	1072
NmuCI	GTSAC	3 cut(s)	354, 683, 1375
NsiI	ATGCAT	1 cut(s)	1650
OliI	CACNNNNGTG	1 cut(s)	1106
PaeR7I	CTCGAG	2 cut(s)	105, 1319
PaqCI	CACCTGC	1 cut(s)	1205
PcsI	WCGNNNNNNNCGW	1 cut(s)	120
PctI	GAATGC	2 cut(s)	721, 1354
PfeI	GAWTC	8 cut(s)	211, 464, 733, 811, 886, 1081, 1126, 1390
PflFI	GACNNNGTC	1 cut(s)	46
PkrI	GCNGC	9 cut(s)	30, 103, 244, 283, 286, 691, 1121, 1275, 1437
PleI	GAGTC	5 cut(s)	55, 380, 517, 690, 997
PluTI	GGCGCC	1 cut(s)	207
PpsI	GAGTC	5 cut(s)	55, 380, 517, 690, 997
PpuMI	RGGWCCY	2 cut(s)	229, 346
Psp124BI	GAGCTC	1 cut(s)	453
Psp1406I	AACGTT	1 cut(s)	876
Psp5II	RGGWCCY	2 cut(s)	229, 346
Psp6I	CCWGG	4 cut(s)	84, 135, 273, 342
PspFI	CCCAGC	1 cut(s)	24
PspGI	CCWGG	4 cut(s)	84, 135, 273, 342
PspN4I	GGNNCC	6 cut(s)	141, 142, 205, 298, 570, 1157
PspOMI	GGGCCC	1 cut(s)	140
PspPI	GGNCC	9 cut(s)	140, 141, 229, 346, 568, 592, 698, 1044, 1098
PspPPI	RGGWCCY	2 cut(s)	229, 346
PspXI	VCTCGAGB	2 cut(s)	105, 1319
PstI	CTGCAG	1 cut(s)	1278
PstNI	CAGNNNCTG	1 cut(s)	917
PsuI	RGATCY	1 cut(s)	951
PsyI	GACNNNGTC	1 cut(s)	46
RsaI	GTAC	2 cut(s)	518, 1559
RsaNI	GTAC	2 cut(s)	517, 1558
RseI	CAYNNNNRTG	2 cut(s)	1106, 1643
SacI	GAGCTC	1 cut(s)	453
SalI	GTCGAC	2 cut(s)	56, 635
SaqAI	TTAA	2 cut(s)	216, 1669
SatI	GCNGC	9 cut(s)	29, 102, 243, 282, 285, 690, 1120, 1274, 1436
Sau3AI	GATC	8 cut(s)	18, 162, 378, 609, 855, 951, 960, 1071
Sau96I	GGNCC	9 cut(s)	140, 141, 229, 346, 568, 592, 698, 1044, 1098
ScaI	AGTACT	1 cut(s)	518
SchI	GAGTC	5 cut(s)	56, 380, 517, 691, 997
ScrFI	CCNGG	8 cut(s)	86, 137, 275, 344, 572, 868, 1043, 1354
SduI	GDGCHC	4 cut(s)	144, 453, 558, 1228
SfaNI	GCATC	7 cut(s)	109, 240, 268, 372, 898, 1156, 1657
SfcI	CTRYAG	1 cut(s)	1274
SfoI	GGCGCC	1 cut(s)	205
Sfr274I	CTCGAG	2 cut(s)	105, 1319
SgrAI	CRCCGGYG	1 cut(s)	692
SinI	GGWCC	5 cut(s)	229, 346, 568, 592, 1098
SlaI	CTCGAG	2 cut(s)	105, 1319
SmiMI	CAYNNNNRTG	2 cut(s)	1106, 1643
SmlI	CTYRAG	2 cut(s)	105, 1319
SmoI	CTYRAG	2 cut(s)	105, 1319
Sse9I	AATT	5 cut(s)	829, 1009, 1365, 1550, 1653
SspDI	GGCGCC	1 cut(s)	203
SspMI	CTAG	1 cut(s)	1139
SstI	GAGCTC	1 cut(s)	453
StyD4I	CCNGG	8 cut(s)	84, 135, 273, 342, 570, 866, 1041, 1352
StyI	CCWWGG	2 cut(s)	144, 432
TaaI	ACNGT	6 cut(s)	307, 339, 376, 461, 634, 1108
TaiI	ACGT	2 cut(s)	423, 879
TaqII	GACCGA	1 cut(s)	59
TasI	AATT	5 cut(s)	829, 1009, 1365, 1550, 1653
TatI	WGTACW	1 cut(s)	516
TauI	GCSGC	6 cut(s)	31, 104, 245, 287, 692, 1122
TfiI	GAWTC	8 cut(s)	211, 464, 733, 811, 886, 1081, 1126, 1390
Tru1I	TTAA	2 cut(s)	216, 1669
Tru9I	TTAA	2 cut(s)	216, 1669
TscAI	CASTG	3 cut(s)	21, 344, 1344
TseFI	GTSAC	3 cut(s)	354, 683, 1375
TseI	GCWGC	3 cut(s)	281, 1273, 1435
Tsp45I	GTSAC	3 cut(s)	354, 683, 1375
TspDTI	ATGAA	1 cut(s)	982
TspGWI	ACGGA	1 cut(s)	550
TspRI	CASTG	3 cut(s)	21, 344, 1344
Tth111I	GACNNNGTC	1 cut(s)	46
VneI	GTGCAC	1 cut(s)	1224
VpaK11BI	GGWCC	5 cut(s)	229, 346, 568, 592, 1098
XapI	RAATTY	1 cut(s)	1550
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1108
XhoI	CTCGAG	2 cut(s)	105, 1319
XmiI	GTMKAC	3 cut(s)	57, 417, 636
XspI	CTAG	1 cut(s)	1139
ZraI	GACGTC	1 cut(s)	421
ZrmI	AGTACT	1 cut(s)	518
Zsp2I	ATGCAT	1 cut(s)	1650

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.