Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0010734

Physical Location & Seq

Genomic Coordinates Reverse (-)

36583052 .. 36586462

Gene Structure 3411 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1234800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGTATATACTGAACGTGGTTGTCTTTTCTCTCCAGAGATTAGAGAGAGAGAGAATGAGGAAGACCATGGCGGTGGGAGTACACTGCATCGTCGCCTTGCTAATTCTTTCCGGTGCGGATCTAGTCAGGTCCGACGGGTCGGATCATCGGTACAAGGAAGGAGATTTTGTCCCTCTGTATGCCAACAAGGTCGGCCCGTTTCACAACCCCAGTGAAACGTACCGATACTTTGATCTCCCATTCTGCTCACGAGATGCGGTGAAAGAGAAAAGGGAAGCTCTTGGTGAAGTATTGAATGGAGATCGTTTAGTCAGTGCCCCTTACAAACTTGAGTTCCTAAGGCATAAAGATACCGAAGTAGCTTGCAAGAAATCACTCACAAAGGAAGAAGTAGCCCGGTTCAGATCTGCTGTCAACAAGGACTATTACTTCCAGATGTATTATGATGACTTGCCCATTTGGGGTTTCATAGGGAAGGTTGACAAGGAAGGCAAAGACCCGAGCGAGTACAAGACCTACCTTTATAAGCACATTCATTTTGATCTCTATTATAACAAGGACCGCGTGATTGAAGTGAATGTTCGAACAGATCCAAATGCACTGGTTGACCTTACAAAGGATAGAGAAGTTGATGTTGAATTCATGTATACTGTGGAGTGGAAGGAAACAACCACTCCTTTCGATAAGAGGATGGATAAGTATTCTCAGTCCTCATCCTTGCCTCATCATTTGGAGATCCATTGGTTCTCAATTATAAATTCATGCGTGACAGTTCTCCTCTTGACCGGGTTTCTTGCTACAATTCTCATGCGAGTACTTAAGAATGATTTTGTCAAATATGCCCATGATGAAGAAGCAGCTGAAGACCAGGAAGAGACTGGATGGAAATACATCCACGGAGATGTATTTAGGTTCCCCAAATACAAGTCCCTGTTTGCAGCAGCTCTAGGTTCAGGAACCCAACTATTTACTCTTACGGTGTTCATTTTCATACTCGCACTTGTTGGTGTGTTTTATCCATACAACCGAGGGGCTTTATTTACTGCTCTGGTTGTCATTTATGCACTCACTTCAGGAATTGCGGGCTATACTGCTACGTCATTTTATTGTCAGCTAGAGGGATCAAACTGGGTTAGGAATCTATTGTTGACGGGTTGCCTCTTCTGTGGACCTCTGTTCCTCACATTCTGCTTTTTGAATACTGTTGCAATTGCTTATACGGCCACTGCTGCCCTTCCATTCGGCACAATTGTGGTTATAGTTCTGATATGGACACTTGTAACATCACCTTTACTGGTGTTAGGTGGGATTGCTGGGAAGAACAGCAAAGCTGAGTTCCAAGCCCCTGTTCGCACCACTAAATATCCCAGAGAGATTCCACCTTTACCTTGGTACCGGGCAACAATCCCTCAAATGGCAATGGCCGGTTTTCTGCCTTTCAGTGCTATATACATTGAACTGTACTACATATTTGCCAGTGTATGGGGTCACAGGATTTACACCATTTACAGCATCTTGTTTATTGTGTTTATCATTCTTCTGATCGTTACTGCTTTCATTACTGTGGCATTGACATATTTCCAACTTGCTGCCGAGGACCATGAGTGGTGGTGGAGGTCTTTCCTCTGTGGTGGGTCTACTGGACTATTTATCTATGCCTATTGTTTGTATTACTACTACGCACGCTCGGATATGTCAGGATTCATGCAAACATCATTCTTCTTTGGTTACATGGCATGCATCTGCTACGGTTTCTTCCTCATGCTGGGGACTGTAGGCTTCCGTGCAGCTTTGCTCTTTGTCCGTCACATATACCGGTCCATCAAGTGTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002790 GO:0003674 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006897 GO:0006909 GO:0007154 GO:0007155 GO:0007165 GO:0008104 GO:0008150 GO:0008283 GO:0009306 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016192 GO:0022610 GO:0023052 GO:0030587 GO:0031224 GO:0031226 GO:0031589 GO:0032502 GO:0032940 GO:0033036 GO:0033299 GO:0034613 GO:0038023 GO:0042886 GO:0044425 GO:0044459 GO:0044464 GO:0045184 GO:0046903 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051703 GO:0051704 GO:0051716 GO:0060089 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071944 GO:0072657 GO:0090702 GO:0098657 GO:0099120

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EMP70

Protein Analysis

612

Amino Acids

70.32

Weight (kDa)

7.84

Isoelectric Point (pI)

37.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EMP70	PF02990	73 - 568	9.3e-181	Endomembrane protein 70

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010734)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37310 AT5G37310
fragaria_vesca	FvH4_5g17920 FvH4_5g17920 FvH4_5g17920
malus_domestica	MD06G1234800.v1.1 MD14G1242000.v1.1
prunus_persica	Prupe.5G239600_v2.0.a1 Prupe.5G239600_v2.0.a1 Prupe.5G239600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0177681
rosa_laevigata	RLG00000005514
rosa_multiflora	Rmu_sc0004786.1_g000001
rosa_roxburghii	Rroxscaffold_3G00275660
rosa_rugosa	Rorug06G0408400
rosa_samantha	Rh7AG008000 Rh7BG008000 Rh7CG008400 Rh7DG008100
rosa_wichuraiana	Rw7G000670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	525, 552, 755
Acc65I	GGTACC	1 cut(s)	1392
AccB1I	GGYRCC	1 cut(s)	1392
AccB7I	CCANNNNNTGG	1 cut(s)	1482
AccI	GTMKAC	2 cut(s)	647, 1637
AccII	CGCG	1 cut(s)	564
AciI	CCGC	5 cut(s)	71, 116, 257, 562, 1082
AclWI	GGATC	5 cut(s)	126, 150, 584, 730, 1129
AcoI	YGGCCR	2 cut(s)	1221, 1422
AcsI	RAATTY	2 cut(s)	638, 757
AcuI	CTGAAG	2 cut(s)	882, 1056
AfaI	GTAC	7 cut(s)	81, 152, 221, 509, 816, 1394, 1463
AfiI	CCNNNNNNNGG	5 cut(s)	461, 616, 1414, 1482, 1765
AflII	CTTAAG	1 cut(s)	818
AgeI	ACCGGT	1 cut(s)	1815
AgsI	TTSAA	5 cut(s)	295, 572, 638, 1198, 1457
AjnI	CCWGG	1 cut(s)	867
AjuI	GAANNNNNNNTTGG	2 cut(s)	1332, 1364
AleI	CACNNNNGTG	1 cut(s)	1250
AluBI	AGCT	7 cut(s)	278, 362, 860, 944, 1114, 1331, 1790
AluI	AGCT	7 cut(s)	278, 362, 860, 944, 1114, 1331, 1790
Alw26I	GTCTC	1 cut(s)	869
AlwI	GGATC	5 cut(s)	126, 150, 584, 730, 1129
Ama87I	CYCGRG	1 cut(s)	499
AoxI	GGCC	3 cut(s)	193, 1221, 1422
ApeKI	GCWGC	6 cut(s)	857, 938, 941, 1229, 1589, 1787
ApoI	RAATTY	2 cut(s)	638, 757
AsiGI	ACCGGT	1 cut(s)	1815
Asp718I	GGTACC	1 cut(s)	1392
AspS9I	GGNCC	6 cut(s)	129, 194, 559, 1169, 1597, 1818
AsuC2I	CCSGG	3 cut(s)	397, 787, 1397
AsuHPI	GGTGA	3 cut(s)	271, 296, 1278
AsuII	TTCGAA	1 cut(s)	583
AvaI	CYCGRG	1 cut(s)	499
AvaII	GGWCC	5 cut(s)	129, 559, 1169, 1597, 1818
AxyI	CCTNAGG	1 cut(s)	338
BaeGI	GKGCMC	1 cut(s)	319
BaeI	ACNNNNGTAYC	4 cut(s)	342, 375, 1376, 1409
BanI	GGYRCC	1 cut(s)	1392
BauI	CACGAG	1 cut(s)	249
BbsI	GAAGAC	2 cut(s)	68, 870
BbvI	GCAGC	6 cut(s)	869, 950, 953, 1216, 1576, 1799
BccI	CCATC	3 cut(s)	685, 876, 1829
BceAI	ACGGC	1 cut(s)	1236
BciT130I	CCWGG	1 cut(s)	869
BcnI	CCSGG	3 cut(s)	397, 787, 1397
BcoDI	GTCTC	1 cut(s)	869
BfaI	CTAG	3 cut(s)	122, 947, 1115
BfmI	CTRYAG	1 cut(s)	1773
BfrI	CTTAAG	1 cut(s)	818
BglII	AGATCT	1 cut(s)	404
BisI	GCNGC	6 cut(s)	858, 939, 942, 1230, 1590, 1788
BlsI	GCNGC	6 cut(s)	859, 940, 943, 1231, 1591, 1789
BmcAI	AGTACT	1 cut(s)	816
Bme1390I	CCNGG	4 cut(s)	397, 787, 869, 1397
Bme18I	GGWCC	5 cut(s)	129, 559, 1169, 1597, 1818
BmeT110I	CYCGRG	1 cut(s)	499
BmgT120I	GGNCC	6 cut(s)	129, 194, 559, 1169, 1597, 1818
BmiI	GGNNCC	3 cut(s)	914, 958, 1394
BmrFI	CCNGG	4 cut(s)	397, 787, 869, 1397
BmrI	ACTGGG	2 cut(s)	204, 1138
BmsI	GCATC	4 cut(s)	96, 244, 1521, 1749
BmuI	ACTGGG	2 cut(s)	204, 1138
BpiI	GAAGAC	2 cut(s)	68, 870
BpmI	CTGGAG	1 cut(s)	17
Bpu14I	TTCGAA	1 cut(s)	583
BpuEI	CTTGAG	1 cut(s)	350
BpuMI	CCSGG	3 cut(s)	397, 787, 1397
BsaJI	CCNNGG	5 cut(s)	66, 895, 1027, 1388, 1593
BsaWI	WCCGGW	2 cut(s)	110, 1815
BsaXI	ACNNNNNCTCC	4 cut(s)	153, 183, 658, 688
Bsc4I	CCNNNNNNNGG	5 cut(s)	461, 616, 1414, 1482, 1765
Bse118I	RCCGGY	2 cut(s)	1424, 1815
Bse1I	ACTGG	7 cut(s)	210, 606, 883, 1133, 1299, 1476, 1645
Bse21I	CCTNAGG	1 cut(s)	338
Bse3DI	GCAATG	1 cut(s)	1425
BseBI	CCWGG	1 cut(s)	869
BseDI	CCNNGG	5 cut(s)	66, 895, 1027, 1388, 1593
BseGI	GGATG	4 cut(s)	696, 713, 887, 891
BseLI	CCNNNNNNNGG	5 cut(s)	461, 616, 1414, 1482, 1765
BseMI	GCAATG	1 cut(s)	1425
BseMII	CTCAG	2 cut(s)	719, 1323
BseNI	ACTGG	7 cut(s)	210, 606, 883, 1133, 1299, 1476, 1645
BseRI	GAGGAG	1 cut(s)	767
BseSI	GKGCMC	1 cut(s)	319
BseXI	GCAGC	6 cut(s)	869, 950, 953, 1216, 1576, 1799
BseYI	CCCAGC	2 cut(s)	1313, 1765
BsgI	GTGCAG	1 cut(s)	1806
Bsh1236I	CGCG	1 cut(s)	564
BshFI	GGCC	3 cut(s)	195, 1223, 1424
BshNI	GGYRCC	1 cut(s)	1392
BshTI	ACCGGT	1 cut(s)	1815
BsiHKCI	CYCGRG	1 cut(s)	499
BsiSI	CCGG	6 cut(s)	111, 397, 786, 1396, 1425, 1816
BslFI	GGGAC	3 cut(s)	155, 913, 1783
BslI	CCNNNNNNNGG	5 cut(s)	461, 616, 1414, 1482, 1765
BsmAI	GTCTC	1 cut(s)	869
BsmFI	GGGAC	3 cut(s)	155, 913, 1783
BsnI	GGCC	3 cut(s)	195, 1223, 1424
BsoBI	CYCGRG	1 cut(s)	499
Bsp119I	TTCGAA	1 cut(s)	583
Bsp1286I	GDGCHC	1 cut(s)	319
Bsp19I	CCATGG	1 cut(s)	66
BspACI	CCGC	5 cut(s)	71, 116, 257, 562, 1082
BspANI	GGCC	3 cut(s)	195, 1223, 1424
BspCNI	CTCAG	2 cut(s)	718, 1324
BspFNI	CGCG	1 cut(s)	564
BspLI	GGNNCC	3 cut(s)	914, 958, 1394
BspPI	GGATC	5 cut(s)	126, 150, 584, 730, 1129
BspT104I	TTCGAA	1 cut(s)	583
BspT107I	GGYRCC	1 cut(s)	1392
BspTI	CTTAAG	1 cut(s)	818
BsrDI	GCAATG	1 cut(s)	1425
BsrFI	RCCGGY	2 cut(s)	1424, 1815
BsrI	ACTGG	7 cut(s)	210, 606, 883, 1133, 1299, 1476, 1645
BssAI	RCCGGY	2 cut(s)	1424, 1815
BssECI	CCNNGG	5 cut(s)	66, 895, 1027, 1388, 1593
BssNAI	GTATAC	1 cut(s)	648
BssSI	CACGAG	1 cut(s)	249
BssT1I	CCWWGG	2 cut(s)	66, 1388
Bst1107I	GTATAC	1 cut(s)	648
Bst2BI	CACGAG	1 cut(s)	249
Bst2UI	CCWGG	1 cut(s)	869
Bst4CI	ACNGT	8 cut(s)	652, 772, 979, 1204, 1461, 1564, 1751, 1774
Bst6I	CTCTTC	2 cut(s)	867, 1166
BstAFI	CTTAAG	1 cut(s)	818
BstBI	TTCGAA	1 cut(s)	583
BstC8I	GCNNGC	4 cut(s)	364, 1084, 1684, 1738
BstDEI	CTNAG	3 cut(s)	338, 705, 1332
BstDSI	CCRYGG	2 cut(s)	66, 895
BstENI	CCTNNNNNAGG	1 cut(s)	614
BstF5I	GGATG	4 cut(s)	696, 713, 887, 891
BstFNI	CGCG	1 cut(s)	564
BstMAI	GTCTC	1 cut(s)	869
BstMWI	GCNNNNNNNGC	2 cut(s)	1220, 1229
BstNI	CCWGG	1 cut(s)	869
BstNSI	RCATGY	1 cut(s)	1740
BstSCI	CCNGG	4 cut(s)	395, 785, 867, 1395
BstSFI	CTRYAG	1 cut(s)	1773
BstSLI	GKGCMC	1 cut(s)	319
BstUI	CGCG	1 cut(s)	564
BstV1I	GCAGC	6 cut(s)	869, 950, 953, 1216, 1576, 1799
BstV2I	GAAGAC	2 cut(s)	68, 870
BstX2I	RGATCY	4 cut(s)	118, 404, 589, 735
BstXI	CCANNNNNNTGG	1 cut(s)	73
BstYI	RGATCY	4 cut(s)	118, 404, 589, 735
BstZ17I	GTATAC	1 cut(s)	648
Bsu36I	CCTNAGG	1 cut(s)	338
BsuRI	GGCC	3 cut(s)	195, 1223, 1424
BtgI	CCRYGG	2 cut(s)	66, 895
BtsCI	GGATG	4 cut(s)	696, 713, 887, 891
BtsI	GCAGTG	2 cut(s)	82, 1224
BtsIMutI	CAGTG	7 cut(s)	82, 217, 319, 599, 1224, 1447, 1483
Cac8I	GCNNGC	4 cut(s)	364, 1084, 1684, 1738
Cfr10I	RCCGGY	2 cut(s)	1424, 1815
Cfr13I	GGNCC	6 cut(s)	129, 194, 559, 1169, 1597, 1818
Csp6I	GTAC	7 cut(s)	80, 151, 220, 508, 815, 1393, 1462
CspAI	ACCGGT	1 cut(s)	1815
CviQI	GTAC	7 cut(s)	80, 151, 220, 508, 815, 1393, 1462
DdeI	CTNAG	3 cut(s)	338, 705, 1332
EaeI	YGGCCR	2 cut(s)	1221, 1422
Eam1104I	CTCTTC	2 cut(s)	867, 1166
EarI	CTCTTC	2 cut(s)	867, 1166
Eco130I	CCWWGG	2 cut(s)	66, 1388
Eco47I	GGWCC	5 cut(s)	129, 559, 1169, 1597, 1818
Eco57I	CTGAAG	2 cut(s)	882, 1056
Eco81I	CCTNAGG	1 cut(s)	338
Eco88I	CYCGRG	1 cut(s)	499
EcoNI	CCTNNNNNAGG	1 cut(s)	614
EcoRI	GAATTC	1 cut(s)	638
EcoRII	CCWGG	1 cut(s)	867
EcoT14I	CCWWGG	2 cut(s)	66, 1388
EcoT22I	ATGCAT	1 cut(s)	1742
ErhI	CCWWGG	2 cut(s)	66, 1388
FaqI	GGGAC	3 cut(s)	155, 913, 1783
FauI	CCCGC	1 cut(s)	1075
FblI	GTMKAC	2 cut(s)	647, 1637
Fnu4HI	GCNGC	6 cut(s)	858, 939, 942, 1230, 1590, 1788
FokI	GGATG	4 cut(s)	700, 703, 878, 894
Fsp4HI	GCNGC	6 cut(s)	858, 939, 942, 1230, 1590, 1788
FspBI	CTAG	3 cut(s)	122, 947, 1115
GluI	GCNGC	6 cut(s)	858, 939, 942, 1230, 1590, 1788
GsaI	CCCAGC	2 cut(s)	1317, 1769
GsuI	CTGGAG	1 cut(s)	17
HaeIII	GGCC	3 cut(s)	195, 1223, 1424
HapII	CCGG	6 cut(s)	111, 397, 786, 1396, 1425, 1816
HincII	GTYRAC	4 cut(s)	415, 481, 607, 1149
HindII	GTYRAC	4 cut(s)	415, 481, 607, 1149
HinfI	GANTC	3 cut(s)	1138, 1375, 1701
HpaII	CCGG	6 cut(s)	111, 397, 786, 1396, 1425, 1816
HphI	GGTGA	3 cut(s)	271, 296, 1278
Hpy166II	GTNNAC	8 cut(s)	82, 415, 481, 607, 648, 1149, 1169, 1638
Hpy188I	TCNGA	6 cut(s)	133, 142, 404, 1266, 1542, 1690
Hpy188III	TCNNGA	7 cut(s)	34, 249, 433, 781, 954, 1074, 1698
Hpy8I	GTNNAC	8 cut(s)	82, 415, 481, 607, 648, 1149, 1169, 1638
Hpy99I	CGWCG	2 cut(s)	95, 137
HpyAV	CCTTC	5 cut(s)	152, 469, 482, 655, 1244
HpyCH4III	ACNGT	8 cut(s)	652, 772, 979, 1204, 1461, 1564, 1751, 1774
HpyCH4IV	ACGT	3 cut(s)	15, 218, 1097
HpyCH4V	TGCA	9 cut(s)	87, 366, 599, 938, 1064, 1208, 1708, 1740, 1787
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1220, 1229
HpyF3I	CTNAG	3 cut(s)	338, 705, 1332
HpySE526I	ACGT	3 cut(s)	15, 218, 1097
KpnI	GGTACC	1 cut(s)	1396
Lsp1109I	GCAGC	6 cut(s)	869, 950, 953, 1216, 1576, 1799
LweI	GCATC	4 cut(s)	96, 244, 1521, 1749
MaeI	CTAG	3 cut(s)	122, 947, 1115
MaeII	ACGT	3 cut(s)	15, 218, 1097
MaeIII	GTNAC	6 cut(s)	766, 1279, 1487, 1546, 1727, 1805
MfeI	CAATTG	2 cut(s)	1209, 1248
MflI	RGATCY	4 cut(s)	118, 404, 589, 735
MhlI	GDGCHC	1 cut(s)	319
MluCI	AATT	8 cut(s)	102, 638, 750, 757, 801, 1077, 1209, 1248
MmeI	TCCRAC	3 cut(s)	120, 156, 1606
Mph1103I	ATGCAT	1 cut(s)	1742
MseI	TTAA	1 cut(s)	819
MslI	CAYNNNNRTG	4 cut(s)	71, 900, 1250, 1562
MspA1I	CMGCKG	1 cut(s)	860
MspCI	CTTAAG	1 cut(s)	818
MspI	CCGG	6 cut(s)	111, 397, 786, 1396, 1425, 1816
MspR9I	CCNGG	4 cut(s)	397, 787, 869, 1397
MunI	CAATTG	2 cut(s)	1209, 1248
MvaI	CCWGG	1 cut(s)	869
MvnI	CGCG	1 cut(s)	564
MwoI	GCNNNNNNNGC	2 cut(s)	1220, 1229
NciI	CCSGG	3 cut(s)	397, 787, 1397
NcoI	CCATGG	1 cut(s)	66
NlaIV	GGNNCC	3 cut(s)	914, 958, 1394
NmeAIII	GCCGAG	1 cut(s)	1618
NmuCI	GTSAC	3 cut(s)	766, 1487, 1805
NsiI	ATGCAT	1 cut(s)	1742
NspI	RCATGY	1 cut(s)	1740
NspV	TTCGAA	1 cut(s)	583
OliI	CACNNNNGTG	1 cut(s)	1250
PaeI	GCATGC	1 cut(s)	1740
PfeI	GAWTC	3 cut(s)	1138, 1375, 1701
PflMI	CCANNNNNTGG	1 cut(s)	1482
PinAI	ACCGGT	1 cut(s)	1815
PkrI	GCNGC	6 cut(s)	859, 940, 943, 1231, 1591, 1789
PsiI	TTATAA	3 cut(s)	525, 552, 755
Psp6I	CCWGG	1 cut(s)	867
PspFI	CCCAGC	2 cut(s)	1313, 1765
PspGI	CCWGG	1 cut(s)	867
PspN4I	GGNNCC	3 cut(s)	914, 958, 1394
PspPI	GGNCC	6 cut(s)	129, 194, 559, 1169, 1597, 1818
PsuI	RGATCY	4 cut(s)	118, 404, 589, 735
PvuII	CAGCTG	1 cut(s)	860
RsaI	GTAC	7 cut(s)	81, 152, 221, 509, 816, 1394, 1463
RsaNI	GTAC	7 cut(s)	80, 151, 220, 508, 815, 1393, 1462
RseI	CAYNNNNRTG	4 cut(s)	71, 900, 1250, 1562
SaqAI	TTAA	1 cut(s)	819
SatI	GCNGC	6 cut(s)	858, 939, 942, 1230, 1590, 1788
Sau96I	GGNCC	6 cut(s)	129, 194, 559, 1169, 1597, 1818
ScaI	AGTACT	1 cut(s)	816
ScrFI	CCNGG	4 cut(s)	397, 787, 869, 1397
SduI	GDGCHC	1 cut(s)	319
SfaNI	GCATC	4 cut(s)	96, 244, 1521, 1749
SfcI	CTRYAG	1 cut(s)	1773
SfuI	TTCGAA	1 cut(s)	583
SinI	GGWCC	5 cut(s)	129, 559, 1169, 1597, 1818
SmiMI	CAYNNNNRTG	4 cut(s)	71, 900, 1250, 1562
SmlI	CTYRAG	2 cut(s)	329, 818
SmoI	CTYRAG	2 cut(s)	329, 818
SphI	GCATGC	1 cut(s)	1740
Sse9I	AATT	8 cut(s)	102, 638, 750, 757, 801, 1077, 1209, 1248
SsiI	CCGC	5 cut(s)	71, 116, 257, 562, 1082
SspMI	CTAG	3 cut(s)	122, 947, 1115
StyD4I	CCNGG	4 cut(s)	395, 785, 867, 1395
StyI	CCWWGG	2 cut(s)	66, 1388
TaaI	ACNGT	8 cut(s)	652, 772, 979, 1204, 1461, 1564, 1751, 1774
TaiI	ACGT	3 cut(s)	18, 221, 1100
TaqI	TCGA	2 cut(s)	583, 681
TasI	AATT	8 cut(s)	102, 638, 750, 757, 801, 1077, 1209, 1248
TatI	WGTACW	4 cut(s)	79, 507, 814, 1461
TfiI	GAWTC	3 cut(s)	1138, 1375, 1701
Tru1I	TTAA	1 cut(s)	819
Tru9I	TTAA	1 cut(s)	819
TscAI	CASTG	7 cut(s)	89, 217, 319, 606, 1231, 1447, 1483
TseFI	GTSAC	3 cut(s)	766, 1487, 1805
TseI	GCWGC	6 cut(s)	857, 938, 941, 1229, 1589, 1787
Tsp45I	GTSAC	3 cut(s)	766, 1487, 1805
TspDTI	ATGAA	9 cut(s)	457, 524, 631, 750, 864, 973, 979, 1546, 1693
TspGWI	ACGGA	3 cut(s)	912, 1772, 1793
TspRI	CASTG	7 cut(s)	89, 217, 319, 606, 1231, 1447, 1483
Van91I	CCANNNNNTGG	1 cut(s)	1482
Vha464I	CTTAAG	1 cut(s)	818
VpaK11BI	GGWCC	5 cut(s)	129, 559, 1169, 1597, 1818
XagI	CCTNNNNNAGG	1 cut(s)	614
XapI	RAATTY	2 cut(s)	638, 757
XceI	RCATGY	1 cut(s)	1740
XcmI	CCANNNNNNNNNTGG	2 cut(s)	875, 1386
XmiI	GTMKAC	2 cut(s)	647, 1637
XspI	CTAG	3 cut(s)	122, 947, 1115
ZrmI	AGTACT	1 cut(s)	816
Zsp2I	ATGCAT	1 cut(s)	1742

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.