MD07G1013300.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0018115
Physical Location & Seq
Forward (+)
1179226 .. 1180862
1637 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1013300.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGTATCAGGGGGACGACATGAATAACCCCAGATTCTGGTCAAAGGTTTGCCTGCACAAGATTGCTAAGTTAACCAAGGAAGCTACAACCGTACGGCGTGTATTAGAATCTTTGTTCCGGTATTTTGATAATGGAGATCTCTGGTCTCCTAAACATGGGCTCGCACTGTCTGTCTTGATGGATATGCAGTTAATAATTGAAAATTGTGGGCAGAACAGGCACTTCATGTTGTCCATCTTTATTAAGCATCTTGATCACAAGAATGTTCTTAAGAACCCAAATATGCAGCTGGACGTTGTTGATGTTGCCACCTCTCTTGCTCGAGAAGCAAAGATCCAATCATCAGTTGCTATAATTGGTGCTCTAAGTGATATGATGAGATACCTGCGGAAAAGTATACATTGCTCACTTGATGATTCAAATTTGGGGGCCGAAGTAATTGAATGGAACCGAAAATTTCGAGCAGCAGTGGATGAGTGCCTTGTGCAGTTGACACATAAGGTAGGAGATGCAGGTCCTGTTCTTGACATGATGGCCGTGATGTTAGAAAACATGTCTAACATTACTGTTATGGCTAGAACCTTGATTTCTACTGTTTACCGTACTGGTCAAGTTGTGGCGACAATACCTAATTTGACATATCAAAACAAGGCAAGAATGCATCAGCTATATCACTTGCTTCAATCTACAATTATTAATGCACAAACTTACCAATTTTCCACATTTCCTGAGGCATTATTTCATCAATTACTCGTAGCTATGGTCTGCATGGACCAGGAAAGCAGGTTGGGCACACACCGCATTATTTCATCTGTTTGCCCTCGTCCTTGTGCTGCTGTGCCCATTACTACAAAGAACAATAATATTGGAAGGACCCTCTCTAGAACTGTATCAGTATTTTCTTCATCAGCTGCGCTCTTTGAAAAATTAAACCATCAATCTCACTCACAGGAAAATATTACTCAAGAGATGAAGGACAAAGTTGTAATTGTGGAAGAAGCAAAGGTTCCTAACGAGTCCATGTTGAACAGATTGAAGTCCAAATTTAGTTCAAGAAGGCATCCATCAGCTACAAGTGAGTCTGTCGGTAACGAGGATGGTTCGGTCTCAGTAATCACTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

375

Amino Acids

42.11

Weight (kDa)

8.89

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018115)

Species Orthologous Gene IDs
malus_domestica MD07G1013300.v1.1
rosa_chinensis RchiOBHm_Chr5g0079171
rosa_laevigata RLG00000030645
rosa_multiflora Rmu_sc0011280.1_g000004
rosa_samantha Rh5BG539500 Rh6BG125300 Rh6CG124000 Rh6CG124100 Rh6CG160700 Rh6CG160800
rosa_wichuraiana Rw0G003100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 393, 503, 774
AccB7I CCANNNNNTGG 1 cut(s) 36
AccI GTMKAC 1 cut(s) 397
AciI CCGC 2 cut(s) 388, 799
AclWI GGATC 1 cut(s) 328
AcoI YGGCCR 1 cut(s) 534
AcsI RAATTY 3 cut(s) 421, 455, 1043
AdeI CACNNNGTG 1 cut(s) 1122
AfaI GTAC 2 cut(s) 93, 604
AfiI CCNNNNNNNGG 2 cut(s) 36, 155
AflII CTTAAG 1 cut(s) 269
AflIII ACRYGT 1 cut(s) 552
AgsI TTSAA 8 cut(s) 200, 420, 443, 683, 923, 1027, 1036, 1053
AjnI CCWGG 1 cut(s) 774
AluBI AGCT 6 cut(s) 83, 289, 667, 758, 911, 1070
AluI AGCT 6 cut(s) 83, 289, 667, 758, 911, 1070
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 2 cut(s) 150, 1111
AlwI GGATC 1 cut(s) 328
AlwNI CAGNNNCTG 2 cut(s) 36, 518
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 2 cut(s) 429, 534
ApeKI GCWGC 4 cut(s) 286, 464, 833, 911
ApoI RAATTY 3 cut(s) 421, 455, 1043
AseI ATTAAT 1 cut(s) 696
AspLEI GCGC 1 cut(s) 916
AspS9I GGNCC 4 cut(s) 429, 515, 772, 873
AvaI CYCGRG 1 cut(s) 321
AvaII GGWCC 3 cut(s) 515, 772, 873
AxyI CCTNAGG 1 cut(s) 729
BaeGI GKGCMC 2 cut(s) 794, 843
BanII GRGCYC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 4 cut(s) 298, 476, 820, 898
BccI CCATC 6 cut(s) 172, 242, 526, 942, 1072, 1091
BceAI ACGGC 2 cut(s) 110, 521
BciT130I CCWGG 1 cut(s) 776
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 150, 1111
BfaI CTAG 2 cut(s) 576, 882
BfrI CTTAAG 1 cut(s) 269
BfuAI ACCTGC 3 cut(s) 393, 503, 774
BglII AGATCT 1 cut(s) 136
BisI GCNGC 4 cut(s) 287, 465, 834, 912
BlsI GCNGC 4 cut(s) 288, 466, 835, 913
Bme1390I CCNGG 1 cut(s) 776
Bme18I GGWCC 3 cut(s) 515, 772, 873
BmeT110I CYCGRG 1 cut(s) 321
BmgT120I GGNCC 4 cut(s) 429, 515, 772, 873
BmiI GGNNCC 4 cut(s) 430, 449, 875, 1008
BmrFI CCNGG 1 cut(s) 776
BmsI GCATC 4 cut(s) 256, 499, 670, 1069
BpuEI CTTGAG 1 cut(s) 948
BsaI GGTCTC 2 cut(s) 150, 1111
BsaJI CCNNGG 1 cut(s) 75
BsaWI WCCGGW 1 cut(s) 117
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 155
Bse1I ACTGG 1 cut(s) 610
Bse21I CCTNAGG 1 cut(s) 729
Bse3DI GCAATG 1 cut(s) 400
BseBI CCWGG 1 cut(s) 776
BseDI CCNNGG 1 cut(s) 75
BseGI GGATG 3 cut(s) 478, 1060, 1102
BseLI CCNNNNNNNGG 2 cut(s) 36, 155
BseMI GCAATG 1 cut(s) 400
BseMII CTCAG 2 cut(s) 720, 1122
BseNI ACTGG 1 cut(s) 610
BseSI GKGCMC 2 cut(s) 794, 843
BseXI GCAGC 4 cut(s) 298, 476, 820, 898
BsgI GTGCAG 2 cut(s) 38, 506
BshFI GGCC 2 cut(s) 431, 536
BsiHKAI GWGCWC 1 cut(s) 364
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 1 cut(s) 118
BsiWI CGTACG 1 cut(s) 91
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 2 cut(s) 36, 155
BsmAI GTCTC 2 cut(s) 150, 1111
BsmFI GGGAC 1 cut(s) 26
BsmI GAATGC 1 cut(s) 663
BsnI GGCC 2 cut(s) 431, 536
Bso31I GGTCTC 2 cut(s) 150, 1111
BsoBI CYCGRG 1 cut(s) 321
Bsp1286I GDGCHC 4 cut(s) 162, 364, 794, 843
Bsp143I GATC 3 cut(s) 136, 253, 333
BspACI CCGC 2 cut(s) 388, 799
BspANI GGCC 2 cut(s) 431, 536
BspCNI CTCAG 2 cut(s) 721, 1121
BspLI GGNNCC 4 cut(s) 430, 449, 875, 1008
BspMI ACCTGC 3 cut(s) 393, 503, 774
BspPI GGATC 1 cut(s) 328
BspTI CTTAAG 1 cut(s) 269
BspTNI GGTCTC 2 cut(s) 150, 1111
BsrDI GCAATG 1 cut(s) 400
BsrI ACTGG 1 cut(s) 610
BssECI CCNNGG 1 cut(s) 75
BssMI GATC 3 cut(s) 136, 253, 333
BssNAI GTATAC 1 cut(s) 398
BssT1I CCWWGG 1 cut(s) 75
Bst1107I GTATAC 1 cut(s) 398
Bst2UI CCWGG 1 cut(s) 776
Bst4CI ACNGT 6 cut(s) 91, 168, 568, 595, 602, 889
BstAFI CTTAAG 1 cut(s) 269
BstC8I GCNNGC 2 cut(s) 53, 162
BstDEI CTNAG 4 cut(s) 66, 365, 729, 1108
BstF5I GGATG 3 cut(s) 478, 1060, 1102
BstHHI GCGC 1 cut(s) 916
BstKTI GATC 3 cut(s) 139, 256, 336
BstMAI GTCTC 2 cut(s) 150, 1111
BstMBI GATC 3 cut(s) 136, 253, 333
BstMWI GCNNNNNNNGC 4 cut(s) 217, 326, 789, 798
BstNI CCWGG 1 cut(s) 776
BstNSI RCATGY 1 cut(s) 556
BstSCI CCNGG 1 cut(s) 774
BstSLI GKGCMC 2 cut(s) 794, 843
BstV1I GCAGC 4 cut(s) 298, 476, 820, 898
BstX2I RGATCY 2 cut(s) 136, 333
BstYI RGATCY 2 cut(s) 136, 333
BstZ17I GTATAC 1 cut(s) 398
Bsu36I CCTNAGG 1 cut(s) 729
BsuRI GGCC 2 cut(s) 431, 536
BtsCI GGATG 3 cut(s) 478, 1060, 1102
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 2 cut(s) 164, 474
BveI ACCTGC 3 cut(s) 393, 503, 774
Cac8I GCNNGC 2 cut(s) 53, 162
CaiI CAGNNNCTG 2 cut(s) 36, 518
CfoI GCGC 1 cut(s) 916
Cfr13I GGNCC 4 cut(s) 429, 515, 772, 873
Csp6I GTAC 2 cut(s) 92, 603
CspCI CAANNNNNGTGG 2 cut(s) 298, 333
CviAII CATG 7 cut(s) 19, 155, 226, 529, 553, 769, 1021
CviQI GTAC 2 cut(s) 92, 603
DdeI CTNAG 4 cut(s) 66, 365, 729, 1108
DpnI GATC 3 cut(s) 138, 255, 335
DpnII GATC 3 cut(s) 136, 253, 333
DraIII CACNNNGTG 1 cut(s) 1122
EaeI YGGCCR 1 cut(s) 534
Eco130I CCWWGG 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 162
Eco31I GGTCTC 2 cut(s) 150, 1111
Eco47I GGWCC 3 cut(s) 515, 772, 873
Eco81I CCTNAGG 1 cut(s) 729
Eco88I CYCGRG 1 cut(s) 321
EcoO109I RGGNCCY 2 cut(s) 515, 873
EcoRII CCWGG 1 cut(s) 774
EcoT14I CCWWGG 1 cut(s) 75
EcoT22I ATGCAT 1 cut(s) 663
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 7 cut(s) 22, 158, 229, 532, 556, 772, 1024
FaqI GGGAC 1 cut(s) 26
FatI CATG 7 cut(s) 18, 154, 225, 528, 552, 768, 1020
FbaI TGATCA 1 cut(s) 253
FblI GTMKAC 1 cut(s) 397
Fnu4HI GCNGC 4 cut(s) 287, 465, 834, 912
FokI GGATG 3 cut(s) 485, 1047, 1109
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 4 cut(s) 287, 465, 834, 912
FspBI CTAG 2 cut(s) 576, 882
GlaI GCGC 1 cut(s) 915
GluI GCNGC 4 cut(s) 287, 465, 834, 912
HaeIII GGCC 2 cut(s) 431, 536
HapII CCGG 1 cut(s) 118
HhaI GCGC 1 cut(s) 916
Hin1II CATG 7 cut(s) 22, 158, 229, 532, 556, 772, 1024
Hin6I GCGC 1 cut(s) 914
HinP1I GCGC 1 cut(s) 914
HincII GTYRAC 2 cut(s) 72, 492
HindII GTYRAC 2 cut(s) 72, 492
HinfI GANTC 5 cut(s) 33, 107, 416, 1016, 1079
HpaI GTTAAC 1 cut(s) 72
HpaII CCGG 1 cut(s) 118
Hpy166II GTNNAC 4 cut(s) 72, 398, 492, 598
Hpy188III TCNNGA 8 cut(s) 175, 251, 323, 524, 728, 882, 965, 1053
Hpy8I GTNNAC 4 cut(s) 72, 398, 492, 598
HpyAV CCTTC 3 cut(s) 864, 967, 1050
HpyCH4III ACNGT 6 cut(s) 91, 168, 568, 595, 602, 889
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 8 cut(s) 55, 187, 286, 487, 512, 661, 701, 768
HpyF10VI GCNNNNNNNGC 4 cut(s) 217, 326, 789, 798
HpyF3I CTNAG 4 cut(s) 66, 365, 729, 1108
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 7 cut(s) 22, 158, 229, 532, 556, 772, 1024
HspAI GCGC 1 cut(s) 914
Ksp22I TGATCA 1 cut(s) 253
KspAI GTTAAC 1 cut(s) 72
Kzo9I GATC 3 cut(s) 136, 253, 333
Lsp1109I GCAGC 4 cut(s) 298, 476, 820, 898
LweI GCATC 4 cut(s) 256, 499, 670, 1069
MaeI CTAG 2 cut(s) 576, 882
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 1 cut(s) 1088
MalI GATC 3 cut(s) 138, 255, 335
MboI GATC 3 cut(s) 136, 253, 333
MboII GAAGA 2 cut(s) 894, 1007
MflI RGATCY 2 cut(s) 136, 333
MhlI GDGCHC 4 cut(s) 162, 364, 794, 843
MlyI GAGTC 2 cut(s) 1025, 1088
MnlI CCTC 5 cut(s) 322, 724, 831, 887, 1087
Mph1103I ATGCAT 1 cut(s) 663
MseI TTAA 6 cut(s) 71, 191, 243, 270, 696, 929
MslI CAYNNNNRTG 1 cut(s) 261
MspA1I CMGCKG 2 cut(s) 289, 911
MspCI CTTAAG 1 cut(s) 269
MspI CCGG 1 cut(s) 118
MspR9I CCNGG 1 cut(s) 776
Mva1269I GAATGC 1 cut(s) 663
MvaI CCWGG 1 cut(s) 776
MwoI GCNNNNNNNGC 4 cut(s) 217, 326, 789, 798
NdeII GATC 3 cut(s) 136, 253, 333
NlaIII CATG 7 cut(s) 22, 158, 229, 532, 556, 772, 1024
NlaIV GGNNCC 4 cut(s) 430, 449, 875, 1008
NsiI ATGCAT 1 cut(s) 663
NspI RCATGY 1 cut(s) 556
PaeR7I CTCGAG 1 cut(s) 321
PciI ACATGT 1 cut(s) 552
PctI GAATGC 1 cut(s) 663
PfeI GAWTC 3 cut(s) 33, 107, 416
Pfl23II CGTACG 1 cut(s) 91
PflMI CCANNNNNTGG 1 cut(s) 36
PkrI GCNGC 4 cut(s) 288, 466, 835, 913
PleI GAGTC 2 cut(s) 1024, 1087
PpsI GAGTC 2 cut(s) 1024, 1087
PpuMI RGGWCCY 2 cut(s) 515, 873
PscI ACATGT 1 cut(s) 552
PshBI ATTAAT 1 cut(s) 696
Psp5II RGGWCCY 2 cut(s) 515, 873
Psp6I CCWGG 1 cut(s) 774
PspGI CCWGG 1 cut(s) 774
PspLI CGTACG 1 cut(s) 91
PspN4I GGNNCC 4 cut(s) 430, 449, 875, 1008
PspPI GGNCC 4 cut(s) 429, 515, 772, 873
PspPPI RGGWCCY 2 cut(s) 515, 873
PstNI CAGNNNCTG 2 cut(s) 36, 518
PsuI RGATCY 2 cut(s) 136, 333
PvuII CAGCTG 2 cut(s) 289, 911
RsaI GTAC 2 cut(s) 93, 604
RsaNI GTAC 2 cut(s) 92, 603
RseI CAYNNNNRTG 1 cut(s) 261
SaqAI TTAA 6 cut(s) 71, 191, 243, 270, 696, 929
SatI GCNGC 4 cut(s) 287, 465, 834, 912
Sau3AI GATC 3 cut(s) 136, 253, 333
Sau96I GGNCC 4 cut(s) 429, 515, 772, 873
SchI GAGTC 2 cut(s) 1025, 1088
ScrFI CCNGG 1 cut(s) 776
SduI GDGCHC 4 cut(s) 162, 364, 794, 843
SfaNI GCATC 4 cut(s) 256, 499, 670, 1069
Sfr274I CTCGAG 1 cut(s) 321
SinI GGWCC 3 cut(s) 515, 772, 873
SlaI CTCGAG 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 261
SmlI CTYRAG 3 cut(s) 269, 321, 963
SmoI CTYRAG 3 cut(s) 269, 321, 963
SsiI CCGC 2 cut(s) 388, 799
SspI AATATT 2 cut(s) 865, 958
SspMI CTAG 2 cut(s) 576, 882
StyD4I CCNGG 1 cut(s) 774
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 6 cut(s) 91, 168, 568, 595, 602, 889
TaiI ACGT 1 cut(s) 297
TaqI TCGA 2 cut(s) 322, 460
TaqII GACCGA 1 cut(s) 1093
TfiI GAWTC 3 cut(s) 33, 107, 416
Tru1I TTAA 6 cut(s) 71, 191, 243, 270, 696, 929
Tru9I TTAA 6 cut(s) 71, 191, 243, 270, 696, 929
TscAI CASTG 2 cut(s) 171, 474
TseI GCWGC 4 cut(s) 286, 464, 833, 911
TspDTI ATGAA 6 cut(s) 35, 214, 731, 798, 894, 986
TspGWI ACGGA 1 cut(s) 1110
TspRI CASTG 2 cut(s) 171, 474
Van91I CCANNNNNTGG 1 cut(s) 36
Vha464I CTTAAG 1 cut(s) 269
VpaK11BI GGWCC 3 cut(s) 515, 772, 873
VspI ATTAAT 1 cut(s) 696
XapI RAATTY 3 cut(s) 421, 455, 1043
XbaI TCTAGA 1 cut(s) 881
XceI RCATGY 1 cut(s) 556
XhoI CTCGAG 1 cut(s) 321
XmiI GTMKAC 1 cut(s) 397
XspI CTAG 2 cut(s) 576, 882
Zsp2I ATGCAT 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.