Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0017312

Physical Location & Seq

Genomic Coordinates Forward (+)

4973222 .. 4978977

Gene Structure 5756 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1053900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1581 bp

ATGATACAAGAAACAAGAAGACTTGCAGATGAGTATGAGATATCAGAGATTTTAGGCAGAGGAGGATTCTCTGTGGTCAGAAAAGGCATCAGCAGAAAGTCAAGCAGCAGCAGTGACAAAACCGATGTGGCAATCAAAACACTCAAAAGGCCGTTTGCACCCTCGAACCCTCCTCCTCTGCCGCCCCACGCCCGGCGGAACGACCAGAATAGCTTTGCTGCTGCCGCTTTCCAAACCCGGAAGCAGGTGTCCATATCGAATGTGCTGCTCACGAATGAAATCCTGGTGATGAGGAAGATTGTTGAGAATGTGTCGCCGCACCCGAACGTGATTGACCTCTATGATGTGTACGAGGATGAGAACGGGGTTCACCTTGTGCTGGAGCTATGTTCTGGAGGGGAACTGTTTGATAGGATTGTGAAGCAAGAAAGGTATTCTGAGGTTGGAGCTGCAGCTGTGGTGAGGCAGATTGCACAAGGCTTGGCAGCTCTTCACCGCTCGAATATTGTTCACCGGGATTTGAAGCCGGAAAACTGCCTCTTCTTGGACAACACCGTTGATTCTCCTTTGAAGATTATGGATTTTGGGCTGAGTTCTGTGGAGGAGTTCACTGACCCTGTTGTGGGACTGTTTGGTTCCATAGATTATGTGTCACCAGAGGCTCTTTCTCAGGGGCAAGTAACTTCCAAGAGCGATATGTGGGCTCTTGGTGTAATCTTATATATCCTTCTCTCCGGGTACCCACCTTTTATTGCTCAGTCGAATCGGCAAAAGCAACAGATGATTATGGCTGGAGAATTCAGTTTCTATGAGAAAACCTGGAAGGGGATTTCTTTGTCAGCAAAGCAATTGATTTCGAGCCTCCTCAAAGTCGACCCTGACAAGAGACCTAGTGCTCAAGAGCTTCTGGACCATCCATGGGTTGTCGGTCTTTCAGCCAGAGAGGATCAAATGGATGCTGAGATTGTATCACGACTGCAGAGTTTTAATGCTCGGCGCAAACTCCGTGCTGCAGCAATAGCAAGCGTGTGGACAAGCTCGATTTTCTTAAGGACAAAGAAGCTAAAATCGTTACTAGGATCCTATGACCTTAAACCAGAGGAAATCAAGAATCTGAGTTCACATTTTAAGAAAATATGTGTAAAAGGTGACAATGCTACTCTTTCTGAATTCGAGGAGGTACTGAAAGCAATGAAAATGTCGTCTCTAATGCCTCTAGCACCCCGAATCTTTGACCTATTTGACAACAATCGTGATGGCACCATTGACATGCGAGAGATACTGTGTGGGTTCTCTAATCTCAAGAACTCACAAGGAGACGATGCTCTCCGCCTGTGCTTCCAGATGTATGATACGGATCGGTCTGGATGCATCAGCAAAGAAGAAGTAGCATCTATGCTCAGGGCTTTACCAGATGACTGCCTTCCAGCTGATATCACCGAACCTGGAAAACTAGATGAAATATTTGATCGGATGGATGCAAACAGTGATGGAAAAGTTACCTTTGATGAGTTCAAAGCTGCCATGCAGAGAGATAGCTCTCTCCAAGATGTAGTCCTCTCCCATCTTCGACCAATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009608 GO:0009877 GO:0009931 GO:0009987 GO:0010857 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04626 ko04921 ko04925 ko05145 map04626 map04921 map04925 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 Pkinase

Protein Analysis

527

Amino Acids

58.79

Weight (kDa)

5.78

Isoelectric Point (pI)

45.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	66 - 308	3.3e-59	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	91 - 303	1.9e-30	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	441 - 510	4.9e-15	EF-hand domain pair
EF-hand_6	PF13405	443 - 470	6e-06	EF-hand domain
EF-hand_1	PF00036	484 - 511	2.3e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017312)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g27182
malus_domestica	MD07G1053900.v1.1
rosa_chinensis	RchiOBHm_Chr7g0218291
rosa_laevigata	RLG00000002380
rosa_multiflora	Rmu_sc0002408.1_g000003 Rmu_sc0004956.1_g000013
rosa_roxburghii	Rroxscaffold_3G00241090
rosa_rugosa	Rorug07G0176200
rosa_samantha	Rh7AG317900 Rh7BG309200 Rh7CG334700 Rh7DG316900
rosa_wichuraiana	Rw7G027000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	235
Acc36I	ACCTGC	1 cut(s)	235
Acc65I	GGTACC	1 cut(s)	738
AccB1I	GGYRCC	2 cut(s)	738, 1259
AccBSI	CCGCTC	1 cut(s)	498
AccI	GTMKAC	1 cut(s)	873
AciI	CCGC	6 cut(s)	182, 196, 225, 317, 496, 1330
AclWI	GGATC	4 cut(s)	954, 1074, 1087, 1365
AcsI	RAATTY	2 cut(s)	797, 1169
AdeI	CACNNNGTG	1 cut(s)	376
AfaI	GTAC	3 cut(s)	350, 740, 1182
AfiI	CCNNNNNNNGG	8 cut(s)	192, 244, 379, 544, 622, 623, 825, 919
AflII	CTTAAG	1 cut(s)	1048
AgsI	TTSAA	3 cut(s)	523, 571, 1516
AjnI	CCWGG	3 cut(s)	282, 818, 1444
Alw21I	GWGCWC	1 cut(s)	898
Alw26I	GTCTC	3 cut(s)	880, 1209, 1311
AlwI	GGATC	4 cut(s)	954, 1074, 1087, 1365
AoxI	GGCC	1 cut(s)	149
ApoI	RAATTY	2 cut(s)	797, 1169
Asp718I	GGTACC	1 cut(s)	738
AspLEI	GCGC	1 cut(s)	999
AspS9I	GGNCC	1 cut(s)	910
AsuC2I	CCSGG	4 cut(s)	193, 238, 515, 736
AsuHPI	GGTGA	8 cut(s)	298, 362, 472, 485, 503, 645, 1160, 1429
AvaII	GGWCC	1 cut(s)	910
BamHI	GGATCC	1 cut(s)	1079
BanI	GGYRCC	2 cut(s)	738, 1259
BanII	GRGCYC	1 cut(s)	706
BbsI	GAAGAC	1 cut(s)	25
Bbv12I	GWGCWC	1 cut(s)	898
BccI	CCATC	5 cut(s)	921, 1250, 1468, 1484, 1572
BceAI	ACGGC	1 cut(s)	136
BcgI	CGANNNNNNTGC	2 cut(s)	247, 281
BciT130I	CCWGG	3 cut(s)	284, 820, 1446
BcnI	CCSGG	4 cut(s)	193, 238, 515, 736
BcoDI	GTCTC	3 cut(s)	880, 1209, 1311
BfaI	CTAG	4 cut(s)	891, 1076, 1217, 1454
BfmI	CTRYAG	3 cut(s)	450, 977, 1011
BfrI	CTTAAG	1 cut(s)	1048
BfuAI	ACCTGC	1 cut(s)	235
Bme1390I	CCNGG	7 cut(s)	193, 238, 284, 515, 736, 820, 1446
Bme18I	GGWCC	1 cut(s)	910
BmgT120I	GGNCC	1 cut(s)	910
BmiI	GGNNCC	4 cut(s)	637, 740, 1081, 1261
BmrFI	CCNGG	7 cut(s)	193, 238, 284, 515, 736, 820, 1446
BmsI	GCATC	7 cut(s)	96, 946, 1312, 1358, 1380, 1400, 1468
BpiI	GAAGAC	1 cut(s)	25
BpmI	CTGGAG	3 cut(s)	401, 414, 813
Bpu10I	CCTNAGC	1 cut(s)	1400
BpuEI	CTTGAG	2 cut(s)	882, 1286
BpuMI	CCSGG	4 cut(s)	193, 238, 515, 736
BsaBI	GATNNNNATC	1 cut(s)	1356
BsaI	GGTCTC	1 cut(s)	880
BsaJI	CCNNGG	1 cut(s)	917
Bsc4I	CCNNNNNNNGG	8 cut(s)	192, 244, 379, 544, 622, 623, 825, 919
Bse3DI	GCAATG	1 cut(s)	1197
Bse8I	GATNNNNATC	1 cut(s)	1356
BseBI	CCWGG	3 cut(s)	284, 820, 1446
BseDI	CCNNGG	1 cut(s)	917
BseGI	GGATG	6 cut(s)	361, 913, 961, 1373, 1479, 1483
BseJI	GATNNNNATC	1 cut(s)	1356
BseLI	CCNNNNNNNGG	8 cut(s)	192, 244, 379, 544, 622, 623, 825, 919
BseMI	GCAATG	1 cut(s)	1197
BseMII	CTCAG	7 cut(s)	429, 581, 683, 770, 951, 1106, 1414
BseRI	GAGGAG	6 cut(s)	75, 162, 165, 617, 854, 1190
BshFI	GGCC	1 cut(s)	151
BshNI	GGYRCC	2 cut(s)	738, 1259
BsiHKAI	GWGCWC	1 cut(s)	898
BsiSI	CCGG	5 cut(s)	193, 238, 514, 527, 735
BslFI	GGGAC	1 cut(s)	639
BslI	CCNNNNNNNGG	8 cut(s)	192, 244, 379, 544, 622, 623, 825, 919
BsmAI	GTCTC	3 cut(s)	880, 1209, 1311
BsmBI	CGTCTC	2 cut(s)	1209, 1311
BsmFI	GGGAC	1 cut(s)	639
BsnI	GGCC	1 cut(s)	151
Bso31I	GGTCTC	1 cut(s)	880
Bsp1286I	GDGCHC	2 cut(s)	706, 898
Bsp143I	GATC	4 cut(s)	946, 1079, 1357, 1468
Bsp19I	CCATGG	1 cut(s)	917
BspACI	CCGC	6 cut(s)	182, 196, 225, 317, 496, 1330
BspANI	GGCC	1 cut(s)	151
BspCNI	CTCAG	7 cut(s)	430, 582, 682, 769, 952, 1107, 1413
BspLI	GGNNCC	4 cut(s)	637, 740, 1081, 1261
BspMAI	CTGCAG	3 cut(s)	454, 981, 1015
BspMI	ACCTGC	1 cut(s)	235
BspPI	GGATC	4 cut(s)	954, 1074, 1087, 1365
BspQI	GCTCTTC	1 cut(s)	495
BspT107I	GGYRCC	2 cut(s)	738, 1259
BspTI	CTTAAG	1 cut(s)	1048
BspTNI	GGTCTC	1 cut(s)	880
BsrBI	CCGCTC	1 cut(s)	498
BsrDI	GCAATG	1 cut(s)	1197
BssECI	CCNNGG	1 cut(s)	917
BssMI	GATC	4 cut(s)	946, 1079, 1357, 1468
BssT1I	CCWWGG	1 cut(s)	917
Bst2UI	CCWGG	3 cut(s)	284, 820, 1446
Bst4CI	ACNGT	5 cut(s)	405, 556, 630, 1284, 1487
Bst6I	CTCTTC	2 cut(s)	495, 545
BstAFI	CTTAAG	1 cut(s)	1048
BstC8I	GCNNGC	1 cut(s)	1024
BstDEI	CTNAG	7 cut(s)	438, 590, 669, 756, 960, 1115, 1400
BstDSI	CCRYGG	1 cut(s)	917
BstF5I	GGATG	6 cut(s)	361, 913, 961, 1373, 1479, 1483
BstHHI	GCGC	1 cut(s)	999
BstKTI	GATC	4 cut(s)	949, 1082, 1360, 1471
BstMAI	GTCTC	3 cut(s)	880, 1209, 1311
BstMBI	GATC	4 cut(s)	946, 1079, 1357, 1468
BstMWI	GCNNNNNNNGC	2 cut(s)	224, 1019
BstNI	CCWGG	3 cut(s)	284, 820, 1446
BstNSI	RCATGY	1 cut(s)	1273
BstSCI	CCNGG	7 cut(s)	191, 236, 282, 513, 734, 818, 1444
BstSFI	CTRYAG	3 cut(s)	450, 977, 1011
BstV2I	GAAGAC	1 cut(s)	25
BstX2I	RGATCY	1 cut(s)	1079
BstYI	RGATCY	1 cut(s)	1079
BsuRI	GGCC	1 cut(s)	151
BtgI	CCRYGG	1 cut(s)	917
BtsCI	GGATG	6 cut(s)	361, 913, 961, 1373, 1479, 1483
BtsI	GCAGTG	1 cut(s)	118
BtsIMutI	CAGTG	3 cut(s)	118, 609, 1492
BveI	ACCTGC	1 cut(s)	235
Cac8I	GCNNGC	1 cut(s)	1024
CfoI	GCGC	1 cut(s)	999
Cfr13I	GGNCC	1 cut(s)	910
Csp6I	GTAC	3 cut(s)	349, 739, 1181
CviAII	CATG	3 cut(s)	918, 1270, 1525
CviQI	GTAC	3 cut(s)	349, 739, 1181
DdeI	CTNAG	7 cut(s)	438, 590, 669, 756, 960, 1115, 1400
DpnI	GATC	4 cut(s)	948, 1081, 1359, 1470
DpnII	GATC	4 cut(s)	946, 1079, 1357, 1468
DraIII	CACNNNGTG	1 cut(s)	376
Eam1104I	CTCTTC	2 cut(s)	495, 545
EarI	CTCTTC	2 cut(s)	495, 545
EciI	GGCGGA	2 cut(s)	211, 1319
Eco130I	CCWWGG	1 cut(s)	917
Eco24I	GRGCYC	1 cut(s)	706
Eco31I	GGTCTC	1 cut(s)	880
Eco32I	GATATC	2 cut(s)	42, 1435
Eco47I	GGWCC	1 cut(s)	910
EcoRI	GAATTC	2 cut(s)	797, 1169
EcoRII	CCWGG	3 cut(s)	282, 818, 1444
EcoRV	GATATC	2 cut(s)	42, 1435
EcoT14I	CCWWGG	1 cut(s)	917
EcoT22I	ATGCAT	1 cut(s)	1373
EcoT38I	GRGCYC	1 cut(s)	706
ErhI	CCWWGG	1 cut(s)	917
Esp3I	CGTCTC	2 cut(s)	1209, 1311
FaeI	CATG	3 cut(s)	921, 1273, 1528
FaqI	GGGAC	1 cut(s)	639
FatI	CATG	3 cut(s)	917, 1269, 1524
FblI	GTMKAC	1 cut(s)	873
FokI	GGATG	6 cut(s)	368, 900, 968, 1380, 1486, 1490
FriOI	GRGCYC	1 cut(s)	706
FspBI	CTAG	4 cut(s)	891, 1076, 1217, 1454
GlaI	GCGC	1 cut(s)	998
GsuI	CTGGAG	3 cut(s)	401, 414, 813
HaeIII	GGCC	1 cut(s)	151
HapII	CCGG	5 cut(s)	193, 238, 514, 527, 735
HhaI	GCGC	1 cut(s)	999
Hin1II	CATG	3 cut(s)	921, 1273, 1528
Hin6I	GCGC	1 cut(s)	997
HinP1I	GCGC	1 cut(s)	997
HincII	GTYRAC	1 cut(s)	874
HindII	GTYRAC	1 cut(s)	874
HinfI	GANTC	5 cut(s)	66, 560, 763, 1111, 1227
HpaII	CCGG	5 cut(s)	193, 238, 514, 527, 735
HphI	GGTGA	8 cut(s)	298, 362, 472, 485, 503, 645, 1160, 1429
Hpy166II	GTNNAC	7 cut(s)	349, 370, 511, 609, 874, 1032, 1121
Hpy188I	TCNGA	6 cut(s)	46, 80, 439, 1116, 1168, 1473
Hpy8I	GTNNAC	7 cut(s)	349, 370, 511, 609, 874, 1032, 1121
HpyAV	CCTTC	3 cut(s)	737, 817, 1433
HpyCH4III	ACNGT	5 cut(s)	405, 556, 630, 1284, 1487
HpyCH4IV	ACGT	1 cut(s)	327
HpyCH4V	TGCA	9 cut(s)	26, 158, 452, 473, 979, 1013, 1371, 1481, 1528
HpyF10VI	GCNNNNNNNGC	2 cut(s)	224, 1019
HpyF3I	CTNAG	7 cut(s)	438, 590, 669, 756, 960, 1115, 1400
HpySE526I	ACGT	1 cut(s)	327
Hsp92II	CATG	3 cut(s)	921, 1273, 1528
HspAI	GCGC	1 cut(s)	997
KpnI	GGTACC	1 cut(s)	742
Kzo9I	GATC	4 cut(s)	946, 1079, 1357, 1468
LguI	GCTCTTC	1 cut(s)	495
LmnI	GCTCC	2 cut(s)	382, 446
LweI	GCATC	7 cut(s)	96, 946, 1312, 1358, 1380, 1400, 1468
MaeI	CTAG	4 cut(s)	891, 1076, 1217, 1454
MaeII	ACGT	1 cut(s)	327
MaeIII	GTNAC	6 cut(s)	113, 651, 679, 1071, 1148, 1498
MalI	GATC	4 cut(s)	948, 1081, 1359, 1470
MbiI	CCGCTC	1 cut(s)	498
MboI	GATC	4 cut(s)	946, 1079, 1357, 1468
MboII	GAAGA	7 cut(s)	30, 307, 482, 532, 583, 1394, 1559
MfeI	CAATTG	1 cut(s)	848
MflI	RGATCY	1 cut(s)	1079
MhlI	GDGCHC	2 cut(s)	706, 898
MluCI	AATT	3 cut(s)	797, 848, 1169
MmeI	TCCRAC	1 cut(s)	424
Mph1103I	ATGCAT	1 cut(s)	1373
MseI	TTAA	4 cut(s)	987, 1049, 1092, 1128
MslI	CAYNNNNRTG	1 cut(s)	1268
MspA1I	CMGCKG	2 cut(s)	455, 1430
MspCI	CTTAAG	1 cut(s)	1048
MspI	CCGG	5 cut(s)	193, 238, 514, 527, 735
MspR9I	CCNGG	7 cut(s)	193, 238, 284, 515, 736, 820, 1446
MunI	CAATTG	1 cut(s)	848
MvaI	CCWGG	3 cut(s)	284, 820, 1446
MwoI	GCNNNNNNNGC	2 cut(s)	224, 1019
NciI	CCSGG	4 cut(s)	193, 238, 515, 736
NcoI	CCATGG	1 cut(s)	917
NdeII	GATC	4 cut(s)	946, 1079, 1357, 1468
NlaIII	CATG	3 cut(s)	921, 1273, 1528
NlaIV	GGNNCC	4 cut(s)	637, 740, 1081, 1261
NmeAIII	GCCGAG	1 cut(s)	973
NmuCI	GTSAC	3 cut(s)	113, 651, 1148
NsiI	ATGCAT	1 cut(s)	1373
NspI	RCATGY	1 cut(s)	1273
PaqCI	CACCTGC	1 cut(s)	235
PciSI	GCTCTTC	1 cut(s)	495
PcsI	WCGNNNNNNNCGW	1 cut(s)	320
PfeI	GAWTC	5 cut(s)	66, 560, 763, 1111, 1227
Psp6I	CCWGG	3 cut(s)	282, 818, 1444
PspGI	CCWGG	3 cut(s)	282, 818, 1444
PspN4I	GGNNCC	4 cut(s)	637, 740, 1081, 1261
PspPI	GGNCC	1 cut(s)	910
PstI	CTGCAG	3 cut(s)	454, 981, 1015
PsuI	RGATCY	1 cut(s)	1079
PvuII	CAGCTG	2 cut(s)	455, 1430
RsaI	GTAC	3 cut(s)	350, 740, 1182
RsaNI	GTAC	3 cut(s)	349, 739, 1181
RseI	CAYNNNNRTG	1 cut(s)	1268
SalI	GTCGAC	1 cut(s)	872
SapI	GCTCTTC	1 cut(s)	495
SaqAI	TTAA	4 cut(s)	987, 1049, 1092, 1128
Sau3AI	GATC	4 cut(s)	946, 1079, 1357, 1468
Sau96I	GGNCC	1 cut(s)	910
ScrFI	CCNGG	7 cut(s)	193, 238, 284, 515, 736, 820, 1446
SduI	GDGCHC	2 cut(s)	706, 898
SfaNI	GCATC	7 cut(s)	96, 946, 1312, 1358, 1380, 1400, 1468
SfcI	CTRYAG	3 cut(s)	450, 977, 1011
SinI	GGWCC	1 cut(s)	910
SmiMI	CAYNNNNRTG	1 cut(s)	1268
SmlI	CTYRAG	3 cut(s)	897, 1048, 1301
SmoI	CTYRAG	3 cut(s)	897, 1048, 1301
Sse9I	AATT	3 cut(s)	797, 848, 1169
SsiI	CCGC	6 cut(s)	182, 196, 225, 317, 496, 1330
SspI	AATATT	2 cut(s)	505, 1464
SspMI	CTAG	4 cut(s)	891, 1076, 1217, 1454
StyD4I	CCNGG	7 cut(s)	191, 236, 282, 513, 734, 818, 1444
StyI	CCWWGG	1 cut(s)	917
TaaI	ACNGT	5 cut(s)	405, 556, 630, 1284, 1487
TaiI	ACGT	1 cut(s)	330
TaqI	TCGA	9 cut(s)	164, 257, 500, 761, 857, 873, 1040, 1173, 1570
TaqII	GACCGA	2 cut(s)	917, 1350
TasI	AATT	3 cut(s)	797, 848, 1169
TauI	GCSGC	3 cut(s)	184, 227, 319
TfiI	GAWTC	5 cut(s)	66, 560, 763, 1111, 1227
Tru1I	TTAA	4 cut(s)	987, 1049, 1092, 1128
Tru9I	TTAA	4 cut(s)	987, 1049, 1092, 1128
TscAI	CASTG	3 cut(s)	118, 616, 1492
TseFI	GTSAC	3 cut(s)	113, 651, 1148
Tsp45I	GTSAC	3 cut(s)	113, 651, 1148
TspDTI	ATGAA	3 cut(s)	291, 1208, 1473
TspGWI	ACGGA	2 cut(s)	995, 1370
TspRI	CASTG	3 cut(s)	118, 616, 1492
Vha464I	CTTAAG	1 cut(s)	1048
VpaK11BI	GGWCC	1 cut(s)	910
XapI	RAATTY	2 cut(s)	797, 1169
XceI	RCATGY	1 cut(s)	1273
XmiI	GTMKAC	1 cut(s)	873
XspI	CTAG	4 cut(s)	891, 1076, 1217, 1454
Zsp2I	ATGCAT	1 cut(s)	1373

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.