MD07G1059000.v1.1

Hemerythrin HHE cation binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0020984
Physical Location & Seq
Reverse (-)
5482996 .. 5487248
4253 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1059000.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGGGATCTGTTTCGCGAAGCGGAAGAAATCGACGGCAGAGATAGTGCCGCAAAAACAACTGACGACAACAGTCCGACTGTACGGCTCTCCCAGCAGCACAAACTACATCCGATTTGCCCTCAAATACAAGGCGGCGGCTGTCTCACTTCGCTGCGTGGTGGTAGCCAGATCTAACGACGACAACGACGACAACGACGACACCGACGAAGATTATAGAAACTTGGAGGTGGCGATGGAGGTGGAGGAGGGCGGGTCATCGTCGGAGCCAGCTGAGAGGGTTTCAGGGCCACCCAATACTCTGCTGCAGTTGATGGAGGACAGATTCCCTCATCCGCCGCTTCTTCTTCAAACGGGGTCGTCCTCTGAAACCCAAACGACGTCGCTGGTGGCGGCGGTTGTGAAGCTGACTGAGCTGCAACACAGGAGCCTGGCGTGGCACTTGGAGAGGTTGGTGAGGTGGGTTACGGATCTGTTGAAGCGTCCGGGTAAAGGAAGAGGAGGGGTGGTTGATCCGACGGTGGGGAGTGCTCGGATGGAGTTAAGGAAGCTGGGGAGGAACTACTCGCAGCTTTTGGAGCTGATGTTGGAACACGCTCAGATGGAGGAGAGACTCCTCTTCCCCATCTTCAACTACTGTGATCCAGGAATCTGTAAAGCTGCGAATGAAGAACACGCAAGGGACCTACCCATCATGAACGGCATCAAAGAAGACATCAAATCCATTGAGGTTCTTGACATTGGGAGTCGTGACTACAAAGAAGCTCTTTCTAACTTTTCTAAGCGCCTCAAATCACTACAGGAGCGTTATAGACAACACTTTACGGAGGAGGAAAGAGAAGTATTGCCGTACATGGAGGCAGCCGAGCTGAGCAAAGAGCAGCAGCGGAGACTATTGGACGAGTGTTTGGATGTGATGCAACAGTCACACTCATCGCATTTGTTCAATTTTCTCCTTGAAGGGCTACTTCCTTGTGAAGCCATGCACTACTTGGACTTGATCTCCATGTCCACCAACAAACAACGAACAACTTCTCTGCTTCACATGATCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hemerythrin

Protein Analysis

352

Amino Acids

40.0

Weight (kDa)

5.77

Isoelectric Point (pI)

51.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hemerythrin PF01814 136 - 284 8.8e-13 Hemerythrin HHE cation binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54290
malus_domestica MD07G1059000.v1.1 MD07G1059300.v1.1
prunus_persica Prupe.2G067800_v2.0.a1
pyrus_communis pycom07g04550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 383
AccII CGCG 1 cut(s) 17
AclWI GGATC 4 cut(s) 14, 477, 506, 635
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 2 cut(s) 83, 851
AfiI CCNNNNNNNGG 2 cut(s) 491, 521
AgsI TTSAA 5 cut(s) 350, 478, 631, 946, 959
AjnI CCWGG 2 cut(s) 429, 643
AluBI AGCT 9 cut(s) 272, 406, 415, 550, 571, 580, 659, 764, 868
AluI AGCT 9 cut(s) 272, 406, 415, 550, 571, 580, 659, 764, 868
Alw21I GWGCWC 1 cut(s) 532
Alw26I GTCTC 3 cut(s) 148, 604, 883
AlwI GGATC 4 cut(s) 14, 477, 506, 635
AoxI GGCC 1 cut(s) 287
ApeKI GCWGC 9 cut(s) 96, 153, 304, 415, 568, 659, 860, 880, 883
Asp700I GAANNNNTTC 1 cut(s) 1030
AspLEI GCGC 1 cut(s) 786
AspS9I GGNCC 2 cut(s) 287, 682
AsuC2I CCSGG 1 cut(s) 486
AsuHPI GGTGA 1 cut(s) 466
AvaII GGWCC 1 cut(s) 682
BbsI GAAGAC 1 cut(s) 717
Bbv12I GWGCWC 1 cut(s) 532
BbvI GCAGC 9 cut(s) 108, 140, 291, 402, 580, 646, 872, 892, 895
BccI CCATC 6 cut(s) 229, 307, 529, 595, 632, 698
BceAI ACGGC 4 cut(s) 51, 100, 715, 832
BciT130I CCWGG 2 cut(s) 431, 645
BclI TGATCA 1 cut(s) 1047
BcnI CCSGG 1 cut(s) 486
BcoDI GTCTC 3 cut(s) 148, 604, 883
BfmI CTRYAG 2 cut(s) 305, 797
BfoI RGCGCY 1 cut(s) 787
BglII AGATCT 1 cut(s) 170
BlpI GCTNAGC 1 cut(s) 869
Bme1390I CCNGG 3 cut(s) 431, 486, 645
Bme18I GGWCC 1 cut(s) 682
BmgT120I GGNCC 2 cut(s) 287, 682
BmiI GGNNCC 3 cut(s) 267, 428, 683
BmrFI CCNGG 3 cut(s) 431, 486, 645
BmsI GCATC 2 cut(s) 711, 906
BoxI GACNNNNGTC 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 717
Bpu1102I GCTNAGC 1 cut(s) 869
BpuMI CCSGG 1 cut(s) 486
BsaHI GRCGYC 1 cut(s) 380
BsaXI ACNNNNNCTCC 8 cut(s) 239, 269, 492, 522, 569, 599, 986, 1016
Bsc4I CCNNNNNNNGG 2 cut(s) 491, 521
BseBI CCWGG 2 cut(s) 431, 645
BseGI GGATG 4 cut(s) 108, 331, 540, 916
BseLI CCNNNNNNNGG 2 cut(s) 491, 521
BseMII CTCAG 4 cut(s) 264, 402, 611, 860
BseRI GAGGAG 5 cut(s) 260, 513, 605, 620, 842
BseXI GCAGC 9 cut(s) 108, 140, 291, 402, 580, 646, 872, 892, 895
BseYI CCCAGC 2 cut(s) 92, 550
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 532
BsiSI CCGG 1 cut(s) 485
BslFI GGGAC 1 cut(s) 695
BslI CCNNNNNNNGG 2 cut(s) 491, 521
BsmAI GTCTC 3 cut(s) 148, 604, 883
BsmFI GGGAC 1 cut(s) 695
BsnI GGCC 1 cut(s) 289
Bsp1286I GDGCHC 1 cut(s) 532
Bsp143I GATC 7 cut(s) 6, 170, 469, 511, 640, 999, 1047
Bsp1720I GCTNAGC 1 cut(s) 869
Bsp68I TCGCGA 1 cut(s) 17
BspANI GGCC 1 cut(s) 289
BspCNI CTCAG 4 cut(s) 265, 403, 610, 861
BspFNI CGCG 1 cut(s) 17
BspHI TCATGA 1 cut(s) 693
BspLI GGNNCC 3 cut(s) 267, 428, 683
BspMAI CTGCAG 1 cut(s) 309
BspPI GGATC 4 cut(s) 14, 477, 506, 635
BssMI GATC 7 cut(s) 6, 170, 469, 511, 640, 999, 1047
BssNI GRCGYC 1 cut(s) 380
Bst2UI CCWGG 2 cut(s) 431, 645
Bst4CI ACNGT 5 cut(s) 73, 81, 520, 638, 924
Bst6I CTCTTC 2 cut(s) 490, 623
BstACI GRCGYC 1 cut(s) 380
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 5 cut(s) 273, 411, 597, 780, 869
BstF5I GGATG 4 cut(s) 108, 331, 540, 916
BstFNI CGCG 1 cut(s) 17
BstH2I RGCGCY 1 cut(s) 787
BstHHI GCGC 1 cut(s) 786
BstKTI GATC 7 cut(s) 9, 173, 472, 514, 643, 1002, 1050
BstMAI GTCTC 3 cut(s) 148, 604, 883
BstMBI GATC 7 cut(s) 6, 170, 469, 511, 640, 999, 1047
BstMWI GCNNNNNNNGC 3 cut(s) 93, 412, 577
BstNI CCWGG 2 cut(s) 431, 645
BstPAI GACNNNNGTC 1 cut(s) 71
BstSCI CCNGG 3 cut(s) 429, 484, 643
BstSFI CTRYAG 2 cut(s) 305, 797
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 9 cut(s) 108, 140, 291, 402, 580, 646, 872, 892, 895
BstV2I GAAGAC 1 cut(s) 717
BstX2I RGATCY 3 cut(s) 6, 170, 469
BstYI RGATCY 3 cut(s) 6, 170, 469
BsuRI GGCC 1 cut(s) 289
BtgZI GCGATG 2 cut(s) 248, 918
BtsCI GGATG 4 cut(s) 108, 331, 540, 916
BtuMI TCGCGA 1 cut(s) 17
Cac8I GCNNGC 1 cut(s) 270
CciI TCATGA 1 cut(s) 693
CfoI GCGC 1 cut(s) 786
Cfr13I GGNCC 2 cut(s) 287, 682
CseI GACGC 1 cut(s) 470
Csp6I GTAC 2 cut(s) 82, 850
CviAII CATG 5 cut(s) 694, 853, 982, 1006, 1045
CviQI GTAC 2 cut(s) 82, 850
DdeI CTNAG 5 cut(s) 273, 411, 597, 780, 869
DpnI GATC 7 cut(s) 8, 172, 471, 513, 642, 1001, 1049
DpnII GATC 7 cut(s) 6, 170, 469, 511, 640, 999, 1047
Eam1104I CTCTTC 2 cut(s) 490, 623
EarI CTCTTC 2 cut(s) 490, 623
EciI GGCGGA 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 682
EcoO109I RGGNCCY 1 cut(s) 682
EcoRII CCWGG 2 cut(s) 429, 643
FaeI CATG 5 cut(s) 697, 856, 985, 1009, 1048
FaiI YATR 7 cut(s) 216, 695, 810, 854, 983, 1007, 1046
FalI AAGNNNNNCTT 4 cut(s) 750, 782, 951, 983
FaqI GGGAC 1 cut(s) 695
FatI CATG 5 cut(s) 693, 852, 981, 1005, 1044
FauI CCCGC 1 cut(s) 245
FbaI TGATCA 1 cut(s) 1047
FokI GGATG 4 cut(s) 95, 318, 547, 923
GlaI GCGC 1 cut(s) 785
GsaI CCCAGC 2 cut(s) 96, 554
HaeII RGCGCY 1 cut(s) 787
HaeIII GGCC 1 cut(s) 289
HapII CCGG 1 cut(s) 485
HgaI GACGC 1 cut(s) 470
HhaI GCGC 1 cut(s) 786
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 5 cut(s) 697, 856, 985, 1009, 1048
Hin6I GCGC 1 cut(s) 784
HinP1I GCGC 1 cut(s) 784
HinfI GANTC 4 cut(s) 324, 612, 648, 745
HpaII CCGG 1 cut(s) 485
HphI GGTGA 1 cut(s) 466
Hpy166II GTNNAC 1 cut(s) 1011
Hpy188I TCNGA 7 cut(s) 77, 113, 265, 367, 516, 534, 600
Hpy188III TCNNGA 4 cut(s) 16, 694, 734, 749
Hpy8I GTNNAC 1 cut(s) 1011
Hpy99I CGWCG 9 cut(s) 37, 182, 191, 200, 209, 265, 382, 385, 520
HpyAV CCTTC 1 cut(s) 953
HpyCH4III ACNGT 5 cut(s) 73, 81, 520, 638, 924
HpyCH4IV ACGT 1 cut(s) 380
HpyCH4V TGCA 4 cut(s) 307, 418, 919, 985
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 412, 577
HpyF3I CTNAG 5 cut(s) 273, 411, 597, 780, 869
HpySE526I ACGT 1 cut(s) 380
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 5 cut(s) 697, 856, 985, 1009, 1048
HspAI GCGC 1 cut(s) 784
Ksp22I TGATCA 1 cut(s) 1047
Kzo9I GATC 7 cut(s) 6, 170, 469, 511, 640, 999, 1047
LmnI GCTCC 4 cut(s) 265, 426, 577, 802
Lsp1109I GCAGC 9 cut(s) 108, 140, 291, 402, 580, 646, 872, 892, 895
LweI GCATC 2 cut(s) 711, 906
MaeII ACGT 1 cut(s) 380
MaeIII GTNAC 3 cut(s) 463, 749, 924
MalI GATC 7 cut(s) 8, 172, 471, 513, 642, 1001, 1049
MboI GATC 7 cut(s) 6, 170, 469, 511, 640, 999, 1047
MboII GAAGA 9 cut(s) 37, 221, 335, 338, 507, 610, 619, 680, 722
MflI RGATCY 3 cut(s) 6, 170, 469
MhlI GDGCHC 1 cut(s) 532
MluCI AATT 1 cut(s) 946
MlyI GAGTC 2 cut(s) 606, 754
MmeI TCCRAC 4 cut(s) 100, 243, 539, 567
MroXI GAANNNNTTC 1 cut(s) 1030
MseI TTAA 1 cut(s) 542
MspA1I CMGCKG 2 cut(s) 272, 886
MspI CCGG 1 cut(s) 485
MspR9I CCNGG 3 cut(s) 431, 486, 645
MvaI CCWGG 2 cut(s) 431, 645
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 3 cut(s) 93, 412, 577
NciI CCSGG 1 cut(s) 486
NdeII GATC 7 cut(s) 6, 170, 469, 511, 640, 999, 1047
NlaIII CATG 5 cut(s) 697, 856, 985, 1009, 1048
NlaIV GGNNCC 3 cut(s) 267, 428, 683
NmeAIII GCCGAG 1 cut(s) 889
NmuCI GTSAC 2 cut(s) 749, 924
NruI TCGCGA 1 cut(s) 17
PagI TCATGA 1 cut(s) 693
PcsI WCGNNNNNNNCGW 6 cut(s) 183, 186, 192, 195, 201, 204
PdmI GAANNNNTTC 1 cut(s) 1030
PfeI GAWTC 2 cut(s) 324, 648
PfoI TCCNGGA 1 cut(s) 643
PleI GAGTC 2 cut(s) 606, 753
PpsI GAGTC 2 cut(s) 606, 753
PpuMI RGGWCCY 1 cut(s) 682
PshAI GACNNNNGTC 1 cut(s) 71
Psp5II RGGWCCY 1 cut(s) 682
Psp6I CCWGG 2 cut(s) 429, 643
PspFI CCCAGC 2 cut(s) 92, 550
PspGI CCWGG 2 cut(s) 429, 643
PspN4I GGNNCC 3 cut(s) 267, 428, 683
PspPI GGNCC 2 cut(s) 287, 682
PspPPI RGGWCCY 1 cut(s) 682
PstI CTGCAG 1 cut(s) 309
PsuI RGATCY 3 cut(s) 6, 170, 469
PvuII CAGCTG 1 cut(s) 272
RruI TCGCGA 1 cut(s) 17
RsaI GTAC 2 cut(s) 83, 851
RsaNI GTAC 2 cut(s) 82, 850
SaqAI TTAA 1 cut(s) 542
Sau3AI GATC 7 cut(s) 6, 170, 469, 511, 640, 999, 1047
Sau96I GGNCC 2 cut(s) 287, 682
SchI GAGTC 2 cut(s) 606, 754
ScrFI CCNGG 3 cut(s) 431, 486, 645
SduI GDGCHC 1 cut(s) 532
SfaNI GCATC 2 cut(s) 711, 906
SfcI CTRYAG 2 cut(s) 305, 797
SinI GGWCC 1 cut(s) 682
Sse9I AATT 1 cut(s) 946
StyD4I CCNGG 3 cut(s) 429, 484, 643
TaaI ACNGT 5 cut(s) 73, 81, 520, 638, 924
TaiI ACGT 1 cut(s) 383
TaqI TCGA 1 cut(s) 32
TasI AATT 1 cut(s) 946
TauI GCSGC 5 cut(s) 52, 137, 140, 340, 395
TfiI GAWTC 2 cut(s) 324, 648
Tru1I TTAA 1 cut(s) 542
Tru9I TTAA 1 cut(s) 542
TseFI GTSAC 2 cut(s) 749, 924
TseI GCWGC 9 cut(s) 96, 153, 304, 415, 568, 659, 860, 880, 883
Tsp45I GTSAC 2 cut(s) 749, 924
TspDTI ATGAA 2 cut(s) 681, 710
TspGWI ACGGA 2 cut(s) 482, 839
VpaK11BI GGWCC 1 cut(s) 682
XcmI CCANNNNNNNNNTGG 1 cut(s) 988
XmnI GAANNNNTTC 1 cut(s) 1030
ZraI GACGTC 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.