MD07G1059300.v1.1

Hemerythrin HHE cation binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0020984
Physical Location & Seq
Reverse (-)
5509661 .. 5512156
2496 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1059300.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGGGGATCTGTTTCGCGAAGCGGAAGAAATCGACGGCAGATATAGTGCCGCAAAAACAACTGACGACAACAGTCCGACTGTACGGCTCTCCCAGCAGCACAAACTACATCCGATTTGCCCTCAAATACAAGGAGGCGGCTGTCTCACTTCGCTGCGTGGTGGTAGCCAGATCTAACGACGACAACGACGACAACGACGACAACGACGACAACGACGAAAACGACGAAGATTATAGAAACTTGGAGGTGGCGATGGAGGTGGAGGAGGGCGGGTCATCGTCGGATCCAGCTGAGAGGGTTTCAGGGCCACCCAATACTCTGCTGCAGTTGATGGAGGCCAGATTCCCTCATCCGCCGCTTCTTCTTCAAACGGGGTCGTCCTCTGAAACCCAAACGACGTCGCTGGTGGCGGCGGTTGTGAAGCTGACTGAGCTGCAACACAGGAGCCTGGCGTGGCACTTGGAGAGGTTGGTGAGGTGGGTTATGGATCTGTTGAAGCGTCCGGGTAAAGGAAGAGGAGGGGTGGTTGATCCGACGGTGGGGAGTGCTCGGATGGAGTTAAGGAAGCTGGGGAGGAACTACTCGCAGCTTTTGGAGCTGATGTTGGAACACGCTCAGATGGAGGAGAGACTCCTCTTCCCCATCTTCAACTACTGTGATCCAGGAATCTGTAAAGCTGCGAATGAAGAACACGCAAGGGACCTACCCATCATGAACGGCATCAAAGAAGACATCAAATCCATTGAGGTTCTTGACATTGGGAGTCGTTACTACAAAGAAGCTCTTTCTAACTTTTCTAAGCGCCTCAATTCACTACAGGAGCGTTATAGACAACACTTTACGGAGGAGGAAAGAGAAGTATTGCCGTACATGGAGGCAGCCGAGCTGAGCAAAGAGCAGCAGCAGAGACTATTGGACGAGTGTTTGGATGTGATGCAACAGTCACACTCATCGCATTTGTTCAATTTTCTCCTTGAAGGGCTACTTCCTTGTGAAGCCATGCACTACTTGGACTTGATCTCCATGTCCACCAACAAACAACGAACAACTTCTCTGCTTCACATGATCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hemerythrin

Protein Analysis

358

Amino Acids

40.74

Weight (kDa)

5.34

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hemerythrin PF01814 142 - 290 3.8e-12 Hemerythrin HHE cation binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54290
malus_domestica MD07G1059000.v1.1 MD07G1059300.v1.1
prunus_persica Prupe.2G067800_v2.0.a1
pyrus_communis pycom07g04550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 401
AccII CGCG 1 cut(s) 17
AciI CCGC 8 cut(s) 22, 50, 137, 270, 353, 356, 410, 413
AclWI GGATC 6 cut(s) 14, 278, 291, 495, 524, 653
AcyI GRCGYC 1 cut(s) 398
AfaI GTAC 2 cut(s) 83, 869
AfiI CCNNNNNNNGG 2 cut(s) 509, 539
AgsI TTSAA 5 cut(s) 368, 496, 649, 964, 977
AjnI CCWGG 2 cut(s) 447, 661
AluBI AGCT 9 cut(s) 290, 424, 433, 568, 589, 598, 677, 782, 886
AluI AGCT 9 cut(s) 290, 424, 433, 568, 589, 598, 677, 782, 886
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 3 cut(s) 148, 622, 901
AlwI GGATC 6 cut(s) 14, 278, 291, 495, 524, 653
AoxI GGCC 2 cut(s) 305, 336
ApeKI GCWGC 9 cut(s) 96, 153, 322, 433, 586, 677, 878, 898, 901
Asp700I GAANNNNTTC 1 cut(s) 1048
AspLEI GCGC 1 cut(s) 804
AspS9I GGNCC 2 cut(s) 305, 700
AsuC2I CCSGG 1 cut(s) 504
AsuHPI GGTGA 1 cut(s) 484
AvaII GGWCC 1 cut(s) 700
BamHI GGATCC 1 cut(s) 283
BbsI GAAGAC 1 cut(s) 735
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 9 cut(s) 108, 140, 309, 420, 598, 664, 890, 910, 913
BccI CCATC 6 cut(s) 247, 325, 547, 613, 650, 716
BceAI ACGGC 4 cut(s) 51, 100, 733, 850
BciT130I CCWGG 2 cut(s) 449, 663
BclI TGATCA 1 cut(s) 1065
BcnI CCSGG 1 cut(s) 504
BcoDI GTCTC 3 cut(s) 148, 622, 901
BfmI CTRYAG 2 cut(s) 323, 815
BfoI RGCGCY 1 cut(s) 805
BglII AGATCT 1 cut(s) 170
BlpI GCTNAGC 1 cut(s) 887
Bme1390I CCNGG 3 cut(s) 449, 504, 663
Bme18I GGWCC 1 cut(s) 700
BmgT120I GGNCC 2 cut(s) 305, 700
BmiI GGNNCC 3 cut(s) 285, 446, 701
BmrFI CCNGG 3 cut(s) 449, 504, 663
BmsI GCATC 2 cut(s) 729, 924
BoxI GACNNNNGTC 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 735
Bpu1102I GCTNAGC 1 cut(s) 887
BpuMI CCSGG 1 cut(s) 504
BsaHI GRCGYC 1 cut(s) 398
BsaXI ACNNNNNCTCC 8 cut(s) 257, 287, 510, 540, 587, 617, 1004, 1034
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 539
BseBI CCWGG 2 cut(s) 449, 663
BseGI GGATG 4 cut(s) 108, 349, 558, 934
BseLI CCNNNNNNNGG 2 cut(s) 509, 539
BseMII CTCAG 4 cut(s) 282, 420, 629, 878
BseRI GAGGAG 5 cut(s) 278, 531, 623, 638, 860
BseXI GCAGC 9 cut(s) 108, 140, 309, 420, 598, 664, 890, 910, 913
BseYI CCCAGC 2 cut(s) 92, 568
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 2 cut(s) 307, 338
BsiHKAI GWGCWC 1 cut(s) 550
BsiSI CCGG 1 cut(s) 503
BslFI GGGAC 1 cut(s) 713
BslI CCNNNNNNNGG 2 cut(s) 509, 539
BsmAI GTCTC 3 cut(s) 148, 622, 901
BsmFI GGGAC 1 cut(s) 713
BsnI GGCC 2 cut(s) 307, 338
Bsp1286I GDGCHC 1 cut(s) 550
Bsp143I GATC 8 cut(s) 6, 170, 283, 487, 529, 658, 1017, 1065
Bsp1720I GCTNAGC 1 cut(s) 887
Bsp68I TCGCGA 1 cut(s) 17
BspACI CCGC 8 cut(s) 22, 50, 137, 270, 353, 356, 410, 413
BspANI GGCC 2 cut(s) 307, 338
BspCNI CTCAG 4 cut(s) 283, 421, 628, 879
BspFNI CGCG 1 cut(s) 17
BspHI TCATGA 1 cut(s) 711
BspLI GGNNCC 3 cut(s) 285, 446, 701
BspMAI CTGCAG 1 cut(s) 327
BspPI GGATC 6 cut(s) 14, 278, 291, 495, 524, 653
BssMI GATC 8 cut(s) 6, 170, 283, 487, 529, 658, 1017, 1065
BssNI GRCGYC 1 cut(s) 398
Bst2UI CCWGG 2 cut(s) 449, 663
Bst4CI ACNGT 5 cut(s) 73, 81, 538, 656, 942
Bst6I CTCTTC 2 cut(s) 508, 641
BstACI GRCGYC 1 cut(s) 398
BstDEI CTNAG 5 cut(s) 291, 429, 615, 798, 887
BstF5I GGATG 4 cut(s) 108, 349, 558, 934
BstFNI CGCG 1 cut(s) 17
BstH2I RGCGCY 1 cut(s) 805
BstHHI GCGC 1 cut(s) 804
BstKTI GATC 8 cut(s) 9, 173, 286, 490, 532, 661, 1020, 1068
BstMAI GTCTC 3 cut(s) 148, 622, 901
BstMBI GATC 8 cut(s) 6, 170, 283, 487, 529, 658, 1017, 1065
BstMWI GCNNNNNNNGC 3 cut(s) 93, 430, 595
BstNI CCWGG 2 cut(s) 449, 663
BstPAI GACNNNNGTC 1 cut(s) 71
BstSCI CCNGG 3 cut(s) 447, 502, 661
BstSFI CTRYAG 2 cut(s) 323, 815
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 9 cut(s) 108, 140, 309, 420, 598, 664, 890, 910, 913
BstV2I GAAGAC 1 cut(s) 735
BstX2I RGATCY 4 cut(s) 6, 170, 283, 487
BstYI RGATCY 4 cut(s) 6, 170, 283, 487
BsuRI GGCC 2 cut(s) 307, 338
BtgZI GCGATG 2 cut(s) 266, 936
BtsCI GGATG 4 cut(s) 108, 349, 558, 934
BtuMI TCGCGA 1 cut(s) 17
CciI TCATGA 1 cut(s) 711
CfoI GCGC 1 cut(s) 804
Cfr13I GGNCC 2 cut(s) 305, 700
CseI GACGC 1 cut(s) 488
Csp6I GTAC 2 cut(s) 82, 868
CviAII CATG 5 cut(s) 712, 871, 1000, 1024, 1063
CviQI GTAC 2 cut(s) 82, 868
DdeI CTNAG 5 cut(s) 291, 429, 615, 798, 887
DpnI GATC 8 cut(s) 8, 172, 285, 489, 531, 660, 1019, 1067
DpnII GATC 8 cut(s) 6, 170, 283, 487, 529, 658, 1017, 1065
Eam1104I CTCTTC 2 cut(s) 508, 641
EarI CTCTTC 2 cut(s) 508, 641
EciI GGCGGA 1 cut(s) 342
Eco47I GGWCC 1 cut(s) 700
EcoO109I RGGNCCY 1 cut(s) 700
EcoRII CCWGG 2 cut(s) 447, 661
FaeI CATG 5 cut(s) 715, 874, 1003, 1027, 1066
FaiI YATR 9 cut(s) 44, 234, 485, 713, 828, 872, 1001, 1025, 1064
FalI AAGNNNNNCTT 4 cut(s) 768, 800, 969, 1001
FaqI GGGAC 1 cut(s) 713
FatI CATG 5 cut(s) 711, 870, 999, 1023, 1062
FauI CCCGC 1 cut(s) 263
FbaI TGATCA 1 cut(s) 1065
FokI GGATG 4 cut(s) 95, 336, 565, 941
GlaI GCGC 1 cut(s) 803
GsaI CCCAGC 2 cut(s) 96, 572
HaeII RGCGCY 1 cut(s) 805
HaeIII GGCC 2 cut(s) 307, 338
HapII CCGG 1 cut(s) 503
HgaI GACGC 1 cut(s) 488
HhaI GCGC 1 cut(s) 804
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 5 cut(s) 715, 874, 1003, 1027, 1066
Hin6I GCGC 1 cut(s) 802
HinP1I GCGC 1 cut(s) 802
HinfI GANTC 4 cut(s) 342, 630, 666, 763
HpaII CCGG 1 cut(s) 503
HphI GGTGA 1 cut(s) 484
Hpy166II GTNNAC 1 cut(s) 1029
Hpy188I TCNGA 7 cut(s) 77, 113, 283, 385, 534, 552, 618
Hpy188III TCNNGA 3 cut(s) 16, 712, 752
Hpy8I GTNNAC 1 cut(s) 1029
HpyAV CCTTC 1 cut(s) 971
HpyCH4III ACNGT 5 cut(s) 73, 81, 538, 656, 942
HpyCH4IV ACGT 1 cut(s) 398
HpyCH4V TGCA 4 cut(s) 325, 436, 937, 1003
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 430, 595
HpyF3I CTNAG 5 cut(s) 291, 429, 615, 798, 887
HpySE526I ACGT 1 cut(s) 398
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 5 cut(s) 715, 874, 1003, 1027, 1066
HspAI GCGC 1 cut(s) 802
Ksp22I TGATCA 1 cut(s) 1065
Kzo9I GATC 8 cut(s) 6, 170, 283, 487, 529, 658, 1017, 1065
LmnI GCTCC 3 cut(s) 444, 595, 820
Lsp1109I GCAGC 9 cut(s) 108, 140, 309, 420, 598, 664, 890, 910, 913
LweI GCATC 2 cut(s) 729, 924
MaeII ACGT 1 cut(s) 398
MaeIII GTNAC 2 cut(s) 767, 942
MalI GATC 8 cut(s) 8, 172, 285, 489, 531, 660, 1019, 1067
MboI GATC 8 cut(s) 6, 170, 283, 487, 529, 658, 1017, 1065
MboII GAAGA 9 cut(s) 37, 239, 353, 356, 525, 628, 637, 698, 740
MflI RGATCY 4 cut(s) 6, 170, 283, 487
MhlI GDGCHC 1 cut(s) 550
MluCI AATT 2 cut(s) 808, 964
MlyI GAGTC 2 cut(s) 624, 772
MmeI TCCRAC 4 cut(s) 100, 261, 557, 585
MroXI GAANNNNTTC 1 cut(s) 1048
MseI TTAA 1 cut(s) 560
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 1 cut(s) 503
MspR9I CCNGG 3 cut(s) 449, 504, 663
MvaI CCWGG 2 cut(s) 449, 663
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 3 cut(s) 93, 430, 595
NciI CCSGG 1 cut(s) 504
NdeII GATC 8 cut(s) 6, 170, 283, 487, 529, 658, 1017, 1065
NlaIII CATG 5 cut(s) 715, 874, 1003, 1027, 1066
NlaIV GGNNCC 3 cut(s) 285, 446, 701
NmeAIII GCCGAG 1 cut(s) 907
NmuCI GTSAC 1 cut(s) 942
NruI TCGCGA 1 cut(s) 17
PagI TCATGA 1 cut(s) 711
PdmI GAANNNNTTC 1 cut(s) 1048
PfeI GAWTC 2 cut(s) 342, 666
PfoI TCCNGGA 1 cut(s) 661
PleI GAGTC 2 cut(s) 624, 771
PpsI GAGTC 2 cut(s) 624, 771
PpuMI RGGWCCY 1 cut(s) 700
PshAI GACNNNNGTC 1 cut(s) 71
Psp5II RGGWCCY 1 cut(s) 700
Psp6I CCWGG 2 cut(s) 447, 661
PspFI CCCAGC 2 cut(s) 92, 568
PspGI CCWGG 2 cut(s) 447, 661
PspN4I GGNNCC 3 cut(s) 285, 446, 701
PspPI GGNCC 2 cut(s) 305, 700
PspPPI RGGWCCY 1 cut(s) 700
PstI CTGCAG 1 cut(s) 327
PsuI RGATCY 4 cut(s) 6, 170, 283, 487
PvuII CAGCTG 1 cut(s) 290
RruI TCGCGA 1 cut(s) 17
RsaI GTAC 2 cut(s) 83, 869
RsaNI GTAC 2 cut(s) 82, 868
SaqAI TTAA 1 cut(s) 560
Sau3AI GATC 8 cut(s) 6, 170, 283, 487, 529, 658, 1017, 1065
Sau96I GGNCC 2 cut(s) 305, 700
SchI GAGTC 2 cut(s) 624, 772
ScrFI CCNGG 3 cut(s) 449, 504, 663
SduI GDGCHC 1 cut(s) 550
SfaNI GCATC 2 cut(s) 729, 924
SfcI CTRYAG 2 cut(s) 323, 815
SinI GGWCC 1 cut(s) 700
Sse9I AATT 2 cut(s) 808, 964
SsiI CCGC 8 cut(s) 22, 50, 137, 270, 353, 356, 410, 413
StyD4I CCNGG 3 cut(s) 447, 502, 661
TaaI ACNGT 5 cut(s) 73, 81, 538, 656, 942
TaiI ACGT 1 cut(s) 401
TaqI TCGA 1 cut(s) 32
TasI AATT 2 cut(s) 808, 964
TauI GCSGC 4 cut(s) 52, 140, 358, 413
TfiI GAWTC 2 cut(s) 342, 666
Tru1I TTAA 1 cut(s) 560
Tru9I TTAA 1 cut(s) 560
TseFI GTSAC 1 cut(s) 942
TseI GCWGC 9 cut(s) 96, 153, 322, 433, 586, 677, 878, 898, 901
Tsp45I GTSAC 1 cut(s) 942
TspDTI ATGAA 2 cut(s) 699, 728
TspGWI ACGGA 1 cut(s) 857
VpaK11BI GGWCC 1 cut(s) 700
XcmI CCANNNNNNNNNTGG 1 cut(s) 1006
XmnI GAANNNNTTC 1 cut(s) 1048
ZraI GACGTC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.