pycom07g04550

Hemerythrin HHE cation binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0020984
Physical Location & Seq
Reverse (-)
3910052 .. 3912183
2132 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g04550.1

Sequence Viewer

Length: 1047 bp
ATGGGGATCTGTTTCGGGAAGCGGAAGAAATCGACGGCGGAGATAGTGCCGCAAAAACAGCCGACGACAACAGTCCGACTGTACGGCTCTCCCAGCAGCACAAACTACATCCGATTTGCCCTCAAATACAAGGAGGCGGCTGTCTCACTTCGCTGCATGGTGGTAGCCAGATCTAATGACGACAACGACGACAATGACGAAGATTATAGAAACTTGGTGGTGGCGATGGAGGTGGAGGAGGGCGGGTCATCGTCGGAGCCAGCTGAGAGGGTTTCAGGGCCGCCCAATACTCTGCTGCAGTTGATAGAGGCCAGATTCCCTCATCCGCCGCTTCTCCTTCAAACGGGGTCGTCCTCTGAAACCCAACCGACGTCGCTGGTGGCGGCGGTGGTGAAGCTGACTGAGCTGCAACACAGGAGCCTGGCGTGGCACTTGGAGAGGTTGGTGAGGTGGGTTATGGATCTGTTGAAGCGTCCGGGTAAAGAAAGAGGAGGGGTGGTTGATCCGACGGTGGGGAGTGCTCGGATGGAGGTAAGGAAGCTGGGGAGGAACTACTCGCAGCTTTTGGAGCTGATGTTGGAACACGCTCAGATGGAGGAGAGACTCCTCTTCCCCATCTTCAACTACTGTGATCCAGGGATCTGTAAAGCTGCGAATGAAGAACACGCAAGGGACCTACCCATCATGAACGGAATCAAAGAAGACATCAAATCCATTGAGGTTCTTGACATTGGAAGTCGTGACTACAAAGAAGCTCTTTCCAACTTTTCTAAGCGCCTCAAATCACTACAGGAGCGTTATAGACAACACTTTACGGAGGAGGAAAGAGAAGTATTGCCGTACATGGAGGCCGCCGAGCTGAGCAAAGAGCAGCAGCAGAGACTATTGGACGAGTGTTTGGATGTGATGCAACAGTCACACTCATCGCATTTGTTCAATTTTCTTCTTGAAGGGCTACTTCCTCGTGAAGCCATGCACTACTTGGAATTAATCTCCATGTCCACCAACAAACAACGAACAACTTCTCTGCTTCACATGATCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hemerythrin

Protein Analysis

349

Amino Acids

39.76

Weight (kDa)

5.99

Isoelectric Point (pI)

53.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hemerythrin PF01814 133 - 281 2.1e-12 Hemerythrin HHE cation binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54290
malus_domestica MD07G1059000.v1.1 MD07G1059300.v1.1
prunus_persica Prupe.2G067800_v2.0.a1
pyrus_communis pycom07g04550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 374
AclWI GGATC 5 cut(s) 14, 468, 497, 626, 647
AcyI GRCGYC 1 cut(s) 371
AfaI GTAC 2 cut(s) 83, 842
AfiI CCNNNNNNNGG 2 cut(s) 343, 512
AgsI TTSAA 5 cut(s) 341, 469, 622, 937, 950
AjnI CCWGG 2 cut(s) 420, 634
AluBI AGCT 9 cut(s) 263, 397, 406, 541, 562, 571, 650, 755, 859
AluI AGCT 9 cut(s) 263, 397, 406, 541, 562, 571, 650, 755, 859
Alw21I GWGCWC 1 cut(s) 523
Alw26I GTCTC 3 cut(s) 148, 595, 874
AlwI GGATC 5 cut(s) 14, 468, 497, 626, 647
AoxI GGCC 3 cut(s) 278, 309, 849
ApeKI GCWGC 8 cut(s) 96, 153, 295, 406, 559, 650, 871, 874
AseI ATTAAT 1 cut(s) 989
Asp700I GAANNNNTTC 1 cut(s) 1021
AspLEI GCGC 1 cut(s) 777
AspS9I GGNCC 2 cut(s) 278, 673
AsuC2I CCSGG 1 cut(s) 477
AsuHPI GGTGA 2 cut(s) 403, 457
AvaII GGWCC 1 cut(s) 673
BauI CACGAG 1 cut(s) 963
BbsI GAAGAC 1 cut(s) 708
Bbv12I GWGCWC 1 cut(s) 523
BbvI GCAGC 8 cut(s) 108, 140, 282, 393, 571, 637, 883, 886
BccI CCATC 5 cut(s) 220, 520, 586, 623, 689
BceAI ACGGC 3 cut(s) 51, 100, 823
BciT130I CCWGG 2 cut(s) 422, 636
BclI TGATCA 1 cut(s) 1038
BcnI CCSGG 1 cut(s) 477
BcoDI GTCTC 3 cut(s) 148, 595, 874
BfmI CTRYAG 2 cut(s) 296, 788
BfoI RGCGCY 1 cut(s) 778
BglII AGATCT 1 cut(s) 170
BlpI GCTNAGC 1 cut(s) 860
Bme1390I CCNGG 3 cut(s) 422, 477, 636
Bme18I GGWCC 1 cut(s) 673
BmgT120I GGNCC 2 cut(s) 278, 673
BmiI GGNNCC 3 cut(s) 258, 419, 674
BmrFI CCNGG 3 cut(s) 422, 477, 636
BmsI GCATC 1 cut(s) 897
BoxI GACNNNNGTC 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 708
Bpu1102I GCTNAGC 1 cut(s) 860
BpuMI CCSGG 1 cut(s) 477
BsaHI GRCGYC 1 cut(s) 371
BsaJI CCNNGG 1 cut(s) 635
BsaXI ACNNNNNCTCC 6 cut(s) 230, 260, 483, 513, 560, 590
Bsc4I CCNNNNNNNGG 2 cut(s) 343, 512
BseBI CCWGG 2 cut(s) 422, 636
BseDI CCNNGG 1 cut(s) 635
BseGI GGATG 4 cut(s) 108, 322, 531, 907
BseLI CCNNNNNNNGG 2 cut(s) 343, 512
BseMII CTCAG 4 cut(s) 255, 393, 602, 851
BseRI GAGGAG 5 cut(s) 251, 504, 596, 611, 833
BseXI GCAGC 8 cut(s) 108, 140, 282, 393, 571, 637, 883, 886
BseYI CCCAGC 2 cut(s) 92, 541
BshFI GGCC 3 cut(s) 280, 311, 851
BsiHKAI GWGCWC 1 cut(s) 523
BsiSI CCGG 1 cut(s) 476
BslFI GGGAC 1 cut(s) 686
BslI CCNNNNNNNGG 2 cut(s) 343, 512
BsmAI GTCTC 3 cut(s) 148, 595, 874
BsmFI GGGAC 1 cut(s) 686
BsnI GGCC 3 cut(s) 280, 311, 851
Bsp1286I GDGCHC 1 cut(s) 523
Bsp143I GATC 7 cut(s) 6, 170, 460, 502, 631, 639, 1038
Bsp1720I GCTNAGC 1 cut(s) 860
BspANI GGCC 3 cut(s) 280, 311, 851
BspCNI CTCAG 4 cut(s) 256, 394, 601, 852
BspHI TCATGA 1 cut(s) 684
BspLI GGNNCC 3 cut(s) 258, 419, 674
BspMAI CTGCAG 1 cut(s) 300
BspPI GGATC 5 cut(s) 14, 468, 497, 626, 647
BssECI CCNNGG 1 cut(s) 635
BssMI GATC 7 cut(s) 6, 170, 460, 502, 631, 639, 1038
BssNI GRCGYC 1 cut(s) 371
BssSI CACGAG 1 cut(s) 963
Bst2BI CACGAG 1 cut(s) 963
Bst2UI CCWGG 2 cut(s) 422, 636
Bst4CI ACNGT 5 cut(s) 73, 81, 511, 629, 915
Bst6I CTCTTC 1 cut(s) 614
BstACI GRCGYC 1 cut(s) 371
BstC8I GCNNGC 1 cut(s) 261
BstDEI CTNAG 5 cut(s) 264, 402, 588, 771, 860
BstF5I GGATG 4 cut(s) 108, 322, 531, 907
BstH2I RGCGCY 1 cut(s) 778
BstHHI GCGC 1 cut(s) 777
BstKTI GATC 7 cut(s) 9, 173, 463, 505, 634, 642, 1041
BstMAI GTCTC 3 cut(s) 148, 595, 874
BstMBI GATC 7 cut(s) 6, 170, 460, 502, 631, 639, 1038
BstMWI GCNNNNNNNGC 4 cut(s) 58, 93, 403, 568
BstNI CCWGG 2 cut(s) 422, 636
BstPAI GACNNNNGTC 1 cut(s) 71
BstSCI CCNGG 3 cut(s) 420, 475, 634
BstSFI CTRYAG 2 cut(s) 296, 788
BstV1I GCAGC 8 cut(s) 108, 140, 282, 393, 571, 637, 883, 886
BstV2I GAAGAC 1 cut(s) 708
BstX2I RGATCY 4 cut(s) 6, 170, 460, 639
BstYI RGATCY 4 cut(s) 6, 170, 460, 639
BsuRI GGCC 3 cut(s) 280, 311, 851
BtgZI GCGATG 2 cut(s) 239, 909
BtsCI GGATG 4 cut(s) 108, 322, 531, 907
Cac8I GCNNGC 1 cut(s) 261
CciI TCATGA 1 cut(s) 684
CfoI GCGC 1 cut(s) 777
Cfr13I GGNCC 2 cut(s) 278, 673
CseI GACGC 1 cut(s) 461
Csp6I GTAC 2 cut(s) 82, 841
CviAII CATG 6 cut(s) 157, 685, 844, 973, 997, 1036
CviQI GTAC 2 cut(s) 82, 841
DdeI CTNAG 5 cut(s) 264, 402, 588, 771, 860
DpnI GATC 7 cut(s) 8, 172, 462, 504, 633, 641, 1040
DpnII GATC 7 cut(s) 6, 170, 460, 502, 631, 639, 1038
Eam1104I CTCTTC 1 cut(s) 614
EarI CTCTTC 1 cut(s) 614
EciI GGCGGA 2 cut(s) 53, 315
Eco47I GGWCC 1 cut(s) 673
EcoO109I RGGNCCY 1 cut(s) 673
EcoRII CCWGG 2 cut(s) 420, 634
FaeI CATG 6 cut(s) 160, 688, 847, 976, 1000, 1039
FaiI YATR 9 cut(s) 158, 207, 458, 686, 801, 845, 974, 998, 1037
FalI AAGNNNNNCTT 4 cut(s) 741, 773, 942, 974
FaqI GGGAC 1 cut(s) 686
FatI CATG 6 cut(s) 156, 684, 843, 972, 996, 1035
FauI CCCGC 1 cut(s) 236
FbaI TGATCA 1 cut(s) 1038
FokI GGATG 4 cut(s) 95, 309, 538, 914
GlaI GCGC 1 cut(s) 776
GsaI CCCAGC 2 cut(s) 96, 545
HaeII RGCGCY 1 cut(s) 778
HaeIII GGCC 3 cut(s) 280, 311, 851
HapII CCGG 1 cut(s) 476
HgaI GACGC 1 cut(s) 461
HhaI GCGC 1 cut(s) 777
Hin1I GRCGYC 1 cut(s) 371
Hin1II CATG 6 cut(s) 160, 688, 847, 976, 1000, 1039
Hin6I GCGC 1 cut(s) 775
HinP1I GCGC 1 cut(s) 775
HinfI GANTC 3 cut(s) 315, 603, 693
HpaII CCGG 1 cut(s) 476
HphI GGTGA 2 cut(s) 403, 457
Hpy166II GTNNAC 1 cut(s) 1002
Hpy188I TCNGA 7 cut(s) 77, 113, 256, 358, 507, 525, 591
Hpy188III TCNNGA 6 cut(s) 16, 685, 725, 740, 947, 965
Hpy8I GTNNAC 1 cut(s) 1002
Hpy99I CGWCG 7 cut(s) 37, 67, 191, 256, 373, 376, 511
HpyAV CCTTC 2 cut(s) 347, 944
HpyCH4III ACNGT 5 cut(s) 73, 81, 511, 629, 915
HpyCH4IV ACGT 1 cut(s) 371
HpyCH4V TGCA 5 cut(s) 156, 298, 409, 910, 976
HpyF10VI GCNNNNNNNGC 4 cut(s) 58, 93, 403, 568
HpyF3I CTNAG 5 cut(s) 264, 402, 588, 771, 860
HpySE526I ACGT 1 cut(s) 371
Hsp92I GRCGYC 1 cut(s) 371
Hsp92II CATG 6 cut(s) 160, 688, 847, 976, 1000, 1039
HspAI GCGC 1 cut(s) 775
Ksp22I TGATCA 1 cut(s) 1038
Kzo9I GATC 7 cut(s) 6, 170, 460, 502, 631, 639, 1038
LmnI GCTCC 4 cut(s) 256, 417, 568, 793
Lsp1109I GCAGC 8 cut(s) 108, 140, 282, 393, 571, 637, 883, 886
LweI GCATC 1 cut(s) 897
MaeII ACGT 1 cut(s) 371
MaeIII GTNAC 2 cut(s) 740, 915
MalI GATC 7 cut(s) 8, 172, 462, 504, 633, 641, 1040
MboI GATC 7 cut(s) 6, 170, 460, 502, 631, 639, 1038
MboII GAAGA 7 cut(s) 37, 212, 601, 610, 671, 713, 935
MflI RGATCY 4 cut(s) 6, 170, 460, 639
MhlI GDGCHC 1 cut(s) 523
MluCI AATT 2 cut(s) 937, 986
MlyI GAGTC 1 cut(s) 597
MmeI TCCRAC 5 cut(s) 100, 234, 530, 558, 786
MroXI GAANNNNTTC 1 cut(s) 1021
MseI TTAA 1 cut(s) 989
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 1 cut(s) 476
MspR9I CCNGG 3 cut(s) 422, 477, 636
MvaI CCWGG 2 cut(s) 422, 636
MwoI GCNNNNNNNGC 4 cut(s) 58, 93, 403, 568
NciI CCSGG 1 cut(s) 477
NdeII GATC 7 cut(s) 6, 170, 460, 502, 631, 639, 1038
NlaIII CATG 6 cut(s) 160, 688, 847, 976, 1000, 1039
NlaIV GGNNCC 3 cut(s) 258, 419, 674
NmeAIII GCCGAG 1 cut(s) 880
NmuCI GTSAC 2 cut(s) 740, 915
PagI TCATGA 1 cut(s) 684
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 195
PdmI GAANNNNTTC 1 cut(s) 1021
PfeI GAWTC 2 cut(s) 315, 693
PleI GAGTC 1 cut(s) 597
PpsI GAGTC 1 cut(s) 597
PpuMI RGGWCCY 1 cut(s) 673
PshAI GACNNNNGTC 1 cut(s) 71
PshBI ATTAAT 1 cut(s) 989
Psp5II RGGWCCY 1 cut(s) 673
Psp6I CCWGG 2 cut(s) 420, 634
PspFI CCCAGC 2 cut(s) 92, 541
PspGI CCWGG 2 cut(s) 420, 634
PspN4I GGNNCC 3 cut(s) 258, 419, 674
PspPI GGNCC 2 cut(s) 278, 673
PspPPI RGGWCCY 1 cut(s) 673
PstI CTGCAG 1 cut(s) 300
PsuI RGATCY 4 cut(s) 6, 170, 460, 639
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 2 cut(s) 83, 842
RsaNI GTAC 2 cut(s) 82, 841
SaqAI TTAA 1 cut(s) 989
Sau3AI GATC 7 cut(s) 6, 170, 460, 502, 631, 639, 1038
Sau96I GGNCC 2 cut(s) 278, 673
SchI GAGTC 1 cut(s) 597
ScrFI CCNGG 3 cut(s) 422, 477, 636
SduI GDGCHC 1 cut(s) 523
SfaNI GCATC 1 cut(s) 897
SfcI CTRYAG 2 cut(s) 296, 788
SinI GGWCC 1 cut(s) 673
Sse9I AATT 2 cut(s) 937, 986
StyD4I CCNGG 3 cut(s) 420, 475, 634
TaaI ACNGT 5 cut(s) 73, 81, 511, 629, 915
TaiI ACGT 1 cut(s) 374
TaqI TCGA 1 cut(s) 32
TasI AATT 2 cut(s) 937, 986
TauI GCSGC 6 cut(s) 52, 140, 283, 331, 386, 854
TfiI GAWTC 2 cut(s) 315, 693
Tru1I TTAA 1 cut(s) 989
Tru9I TTAA 1 cut(s) 989
TseFI GTSAC 2 cut(s) 740, 915
TseI GCWGC 8 cut(s) 96, 153, 295, 406, 559, 650, 871, 874
Tsp45I GTSAC 2 cut(s) 740, 915
TspDTI ATGAA 2 cut(s) 672, 701
TspGWI ACGGA 2 cut(s) 705, 830
VpaK11BI GGWCC 1 cut(s) 673
VspI ATTAAT 1 cut(s) 989
XcmI CCANNNNNNNNNTGG 1 cut(s) 979
XmnI GAANNNNTTC 1 cut(s) 1021
ZraI GACGTC 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.