MD07G1104300.v1.1

ARABIDILLO 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010465
Physical Location & Seq
Forward (+)
11814328 .. 11823664
9337 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1104300.v1.1.491

Sequence Viewer

Length: 2802 bp
ATGAGTCGTAGGGTGCGGCGAAAGGTGGCTAGGAAGGGGAAGGAGAAGGTGGTTTTGCCTAGTTATCCGGAAATTGAAGATGAAGTTTCATGTCCAATGCAAAATGGGATTGTCAATTGGACTGGTTTGCCAGATGATACAGTCATTCAGTTGTTTTCATGTCTGAATTATCGTGACCGGGCAAGCTTATCTTCAACTTGCAAGACATGGAGGGTTCTTGGAATTTCGCCTTGTTTGTGGACGTCTTTGGATCTTCGCGCTCACAAATGCAATGATACCATGGCATCTTCACTTGCTTCAAGATGTGTAAATCTTCAGAAGCTTAGGTTTCGTGGGGCAGAATCTGCTGATGCAATATTGCATCTTCGGGCAAAGAATTTACGTGAAATAAGTGGCGATTACTGTAGAAAAATAACTGATGCTACTCTATCTGTGATCGTGGCGCGGCATGAGTCACTTGAAAGCCTCCAGCTTGGGCCAGATTTCTGTGAAAGGATCAGCAGTGATGCTATAAAAGCAATAGCTCTTTGCTGTCCTAAATTGAAAAAGCTTCGACTTTCCGGAATTAGGGATGTGCAAGCTGATGCTATTAATGCTTTATCTAAGCATTGTCCAAATTTGACTGATATCGGGTTCATAGACTGTCTGAACATTGACGAGATGGCACTGGGAAATGTTTTATCAGTTCGATTCCTCTCTGTTGCTGGGACCTCAAACATGAAATGGGGCGTGGTTTCACATCTTTGGCATAAGTTGCCTAACTTGGTTGGGCTAGATGTTTCAAGAACTGATATTGGTTCTGCTGCTGTTTCACGGTTGTTATCATCATCACAGAGCTTGAAGGTTTTATGTGCCCTGAATTGTCCCGTGCTTGAAGAAGACAGCAACTTTGCTCCTCGTAAATATAAAAATAAAATGTTACTTGCCCTTTTTACAGATATTATGAAAGAAATAGCTTTTTTATTAGTCGACATTACGAAGAAAGGAAATAATGTGTTTCTGTATTGGAGGAATTCCAAGAACAAGGATAAAAACTTGAATGACATTATGAGTTGGATTGAATGGATTCTCTCTCATACTCTTTTGCGCATTGCCGAGAGCAATCAACAGGGTTTAGATGTTTTTTGGCTCAAGCAAGGTGCATCTTTGTTGCTCACTTTAATGCAGAGCTCACAAGAGGATGTCCAAGAAAGGGCAGCTACAGGGCTTGCAACTTTTGTTGTTATTGATGATGAAAATGCTAGCATAGATTGTGGGAGGGCTGAAGCTGTTATGCGAGATGGTGGCATACGTCTTCTCTTAAACCTGGCAAAATCTTGGCGGGAAGGGCTCCAATCAGAGGCTGCAAAGGCTATAGCAAACTTGTCTGTGAATGCCAATGTTGCTAAAGCTGTTGCTGAAGAAGGAGGGATCAATATTCTTGCGGGTTTGGCAAGGTCTATGAACAGGCTGGTCGCTGAAGAGGCTGCTGGAGGACTATGGAATCTTTCTGTTGGAGAAGAGCACAAGGGTGCCATTGCTGAGGCCGGTGGAGTGAAAGCTTTAGTTGATCTAATTTTTAAATGGTCATCTGGTGGTGATGGAGTTCTGGAACGGGCAGCTGGTGCACTGGCGAATTTGGCTGCTGATGACAAGTGTAGCACAGAGGTTGCTGTGGCAGGTGGTGTGCATGCTTTAGTGATGCTTGCTCGTAACTGCAAGTTTGAGGGAGTGCAAGAGCAGGTGTACATCTTGCATTGCAAATTATTAATGCACTTCTTATACCAGGCTGCCCGTGCCTTGGCAAATTTGGCTGCCCATGGAGACAGTAATAGTAATAATGCTGCAGTTGGACAAGAGGCAGGTGCTCTTGATGCTCTTGTTCAGCTTACACAATCTCCTCATGAAGGTGTCAGGCAAGAGGCAGCTGGTGCATTGTGGAATCTATCGTTTGATGACAGAAATCGAGAAGCAATTGCTGCAGCAGGCGGTGTTGAGGCATTGGTTGCTCTTGCACAAGGCTGCTCAAACGCCTCCCCAGGCCTTCAAGAACGGGCTGCTGGTGCTCTTTGGGGATTGTCAGTGTCAGAAGCCAATAGCATTGCCATTGGACGGGAAGGAGGTGTTGTGCCTTTGATTGCATTGGCACGCTCTGAAGCTGCAGATGTCCATGAAACAGCTGCGGGTGCTCTTTGGAATCTTGCTTTTAACCCTGGTAATGCTCTCCGGATTGTGGAGGAAGGAGGTGTTCCTGCGCTTGTTCATCTTTGTTCTTCTTCGGTGTCAAAAATGGCTCGCTTCATGGCTGCGTTGGCATTGGCATACATGTTTGATGGCAGAATGGATGAGTACGCGATGATAGGGACTTCATCGGAAAGTGTCTCAAAGAGTGTGAGCTTAGATGGCGCTAGAAGGATGGCTCTGAAACACATTGAGGCCTTTGTCCACACTTTTTCTGATCCACAAACGTTTGGTGCTGCTGCAGTATCATCAGCCCCTGCAGCGTTGGCGCAAGTGACAGAAGGTGCTCGTATTCAAGAAGCAGGACATCTGAGATGCAGCGGGGCTGAAATTGGAAGATTTGTTTCCATGCTACGGAATACATCATCTGTTCTCAAGGCGTGTGCTGCATTTGCTCTTCTTCAGTTTACCATACCTGGTGGCCGGCATGCTATGCACCATGCCAGCCTTATGCAGAATGGAGGAGCAGCACGCCTTCTGCGGGCTGCAGCTGCTGCAGCAACTGCCCCCCTTGAAGCCAAAATCTTTGCTAGAATTGTGCTTCGGAATCTTGAGCATCACCAAATAGAACCATCACTTTAA

Protein Analysis

934

Amino Acids

100.04

Weight (kDa)

7.54

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 40 - 86 2.5e-09 F-box-like
F-box PF00646 40 - 80 4.5e-07 F-box domain
Arm PF00514 501 - 542 5.4e-06 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 588 - 726 1.6e-07 ARMC5
Arm PF00514 607 - 643 5.6e-09 Armadillo/beta-catenin-like repeat
Arm PF00514 690 - 728 1.8e-06 Armadillo/beta-catenin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1649, 1711, 1832
AatII GACGTC 1 cut(s) 245
Acc16I TGCGCA 1 cut(s) 1088
Acc36I ACCTGC 3 cut(s) 1649, 1711, 1832
AccB1I GGYRCC 1 cut(s) 1511
AccI GTMKAC 1 cut(s) 969
AccII CGCG 3 cut(s) 258, 445, 2333
AccIII TCCGGA 3 cut(s) 67, 560, 2205
AciI CCGC 8 cut(s) 16, 445, 1321, 1424, 1968, 2162, 2541, 2701
AclI AACGTT 1 cut(s) 2447
AclWI GGATC 4 cut(s) 258, 503, 1418, 2432
AcoI YGGCCR 1 cut(s) 2641
AcsI RAATTY 6 cut(s) 222, 376, 616, 1012, 1615, 1786
AcuI CTGAAG 6 cut(s) 299, 1284, 1419, 1479, 2154, 2606
AcyI GRCGYC 1 cut(s) 242
AfaI GTAC 2 cut(s) 1727, 2330
AfiI CCNNNNNNNGG 9 cut(s) 567, 1192, 1339, 1780, 1886, 2091, 2212, 2574, 2701
AflIII ACRYGT 1 cut(s) 2304
AjnI CCWGG 5 cut(s) 1305, 1764, 2017, 2191, 2635
AleI CACNNNNGTG 1 cut(s) 1509
Alw21I GWGCWC 7 cut(s) 1172, 1506, 1609, 1849, 2047, 2170, 2509
Alw26I GTCTC 2 cut(s) 1797, 2365
Alw44I GTGCAC 1 cut(s) 1605
AlwI GGATC 4 cut(s) 258, 503, 1418, 2432
AlwNI CAGNNNCTG 5 cut(s) 344, 1343, 2477, 2714, 2723
Aor13HI TCCGGA 3 cut(s) 67, 560, 2205
AoxI GGCC 5 cut(s) 476, 1524, 2020, 2415, 2641
ApaLI GTGCAC 1 cut(s) 1605
ApoI RAATTY 6 cut(s) 222, 376, 616, 1012, 1615, 1786
ArsI GACNNNNNNTTYG 2 cut(s) 872, 904
AseI ATTAAT 2 cut(s) 591, 1748
Asp700I GAANNNNTTC 1 cut(s) 1065
AspLEI GCGC 6 cut(s) 260, 445, 1089, 2236, 2387, 2491
AspS9I GGNCC 2 cut(s) 476, 708
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 2 cut(s) 1589, 2771
AsuNHI GCTAGC 1 cut(s) 1241
AvaII GGWCC 1 cut(s) 708
BaeGI GKGCMC 2 cut(s) 856, 1609
BanI GGYRCC 1 cut(s) 1511
BanII GRGCYC 2 cut(s) 1172, 1332
BbsI GAAGAC 2 cut(s) 885, 1286
Bbv12I GWGCWC 7 cut(s) 1172, 1506, 1609, 1849, 2047, 2170, 2509
BbvCI CCTCAGC 1 cut(s) 1521
BccI CCATC 7 cut(s) 655, 1274, 1574, 2306, 2375, 2389, 2800
BciT130I CCWGG 5 cut(s) 1307, 1766, 2019, 2193, 2637
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 2 cut(s) 1797, 2365
BfaI CTAG 6 cut(s) 30, 60, 773, 1242, 2388, 2751
BfoI RGCGCY 1 cut(s) 2388
BfuAI ACCTGC 3 cut(s) 1649, 1711, 1832
Bme1390I CCNGG 6 cut(s) 179, 1307, 1766, 2019, 2193, 2637
Bme18I GGWCC 1 cut(s) 708
BmgT120I GGNCC 2 cut(s) 476, 708
BmiI GGNNCC 3 cut(s) 709, 1331, 1513
BmrFI CCNGG 6 cut(s) 179, 1307, 1766, 2019, 2193, 2637
BmrI ACTGGG 1 cut(s) 677
BmtI GCTAGC 1 cut(s) 1245
BmuI ACTGGG 1 cut(s) 677
BpiI GAAGAC 2 cut(s) 885, 1286
BpmI CTGGAG 2 cut(s) 452, 1491
Bpu10I CCTNAGC 2 cut(s) 323, 1521
BpuEI CTTGAG 3 cut(s) 1115, 2579, 2792
BpuMI CCSGG 1 cut(s) 179
BsaAI YACGTR 1 cut(s) 383
BsaHI GRCGYC 1 cut(s) 242
BsaJI CCNNGG 5 cut(s) 279, 1779, 1798, 2017, 2191
BsaWI WCCGGW 3 cut(s) 67, 560, 2205
BsaXI ACNNNNNCTCC 4 cut(s) 1861, 1891, 2091, 2121
Bsc4I CCNNNNNNNGG 9 cut(s) 567, 1192, 1339, 1780, 1886, 2091, 2212, 2574, 2701
Bse118I RCCGGY 2 cut(s) 1526, 2643
Bse1I ACTGG 3 cut(s) 127, 672, 1614
Bse3DI GCAATG 5 cut(s) 277, 1089, 1515, 1735, 2079
BseAI TCCGGA 3 cut(s) 67, 560, 2205
BseBI CCWGG 5 cut(s) 1307, 1766, 2019, 2193, 2637
BseDI CCNNGG 5 cut(s) 279, 1779, 1798, 2017, 2191
BseGI GGATG 4 cut(s) 577, 1186, 2329, 2400
BseLI CCNNNNNNNGG 9 cut(s) 567, 1192, 1339, 1780, 1886, 2091, 2212, 2574, 2701
BseMI GCAATG 5 cut(s) 277, 1089, 1515, 1735, 2079
BseMII CTCAG 2 cut(s) 1512, 2522
BseNI ACTGG 3 cut(s) 127, 672, 1614
BseRI GAGGAG 3 cut(s) 885, 1869, 2697
BseSI GKGCMC 2 cut(s) 856, 1609
BseYI CCCAGC 1 cut(s) 704
Bsh1236I CGCG 3 cut(s) 258, 445, 2333
BshFI GGCC 5 cut(s) 478, 1526, 2022, 2417, 2643
BshNI GGYRCC 1 cut(s) 1511
BsiHKAI GWGCWC 7 cut(s) 1172, 1506, 1609, 1849, 2047, 2170, 2509
BsiSI CCGG 6 cut(s) 68, 178, 561, 1527, 2206, 2644
BslFI GGGAC 3 cut(s) 721, 849, 2356
BslI CCNNNNNNNGG 9 cut(s) 567, 1192, 1339, 1780, 1886, 2091, 2212, 2574, 2701
BsmAI GTCTC 2 cut(s) 1797, 2365
BsmFI GGGAC 3 cut(s) 721, 849, 2356
BsmI GAATGC 1 cut(s) 1378
BsnI GGCC 5 cut(s) 478, 1526, 2022, 2417, 2643
Bsp1286I GDGCHC 9 cut(s) 856, 1172, 1332, 1506, 1609, 1849, 2047, 2170, 2509
Bsp13I TCCGGA 3 cut(s) 67, 560, 2205
Bsp1407I TGTACA 1 cut(s) 1725
Bsp143I GATC 6 cut(s) 250, 435, 495, 1410, 1549, 2437
Bsp19I CCATGG 2 cut(s) 279, 1798
BspACI CCGC 8 cut(s) 16, 445, 1321, 1424, 1968, 2162, 2541, 2701
BspANI GGCC 5 cut(s) 478, 1526, 2022, 2417, 2643
BspCNI CTCAG 2 cut(s) 1513, 2523
BspEI TCCGGA 3 cut(s) 67, 560, 2205
BspFNI CGCG 3 cut(s) 258, 445, 2333
BspHI TCATGA 1 cut(s) 1882
BspLI GGNNCC 3 cut(s) 709, 1331, 1513
BspMAI CTGCAG 7 cut(s) 1828, 1963, 2143, 2464, 2482, 2710, 2719
BspMI ACCTGC 3 cut(s) 1649, 1711, 1832
BspOI GCTAGC 1 cut(s) 1245
BspPI GGATC 4 cut(s) 258, 503, 1418, 2432
BspQI GCTCTTC 2 cut(s) 1494, 2622
BspT107I GGYRCC 1 cut(s) 1511
BsrDI GCAATG 5 cut(s) 277, 1089, 1515, 1735, 2079
BsrFI RCCGGY 2 cut(s) 1526, 2643
BsrGI TGTACA 1 cut(s) 1725
BsrI ACTGG 3 cut(s) 127, 672, 1614
BssAI RCCGGY 2 cut(s) 1526, 2643
BssECI CCNNGG 5 cut(s) 279, 1779, 1798, 2017, 2191
BssMI GATC 6 cut(s) 250, 435, 495, 1410, 1549, 2437
BssNI GRCGYC 1 cut(s) 242
BssT1I CCWWGG 3 cut(s) 279, 1779, 1798
Bst2UI CCWGG 5 cut(s) 1307, 1766, 2019, 2193, 2637
Bst4CI ACNGT 5 cut(s) 142, 404, 644, 816, 1808
Bst6I CTCTTC 3 cut(s) 1455, 1494, 2622
BstACI GRCGYC 1 cut(s) 242
BstAPI GCANNNNNTGC 9 cut(s) 344, 754, 1604, 1910, 1958, 1985, 2653, 2714, 2723
BstAUI TGTACA 1 cut(s) 1725
BstBAI YACGTR 1 cut(s) 383
BstDEI CTNAG 5 cut(s) 323, 603, 1521, 2377, 2531
BstDSI CCRYGG 2 cut(s) 279, 1798
BstENI CCTNNNNNAGG 1 cut(s) 1884
BstF5I GGATG 4 cut(s) 577, 1186, 2329, 2400
BstFNI CGCG 3 cut(s) 258, 445, 2333
BstH2I RGCGCY 1 cut(s) 2388
BstHHI GCGC 6 cut(s) 260, 445, 1089, 2236, 2387, 2491
BstKTI GATC 6 cut(s) 253, 438, 498, 1413, 1552, 2440
BstMAI GTCTC 2 cut(s) 1797, 2365
BstMBI GATC 6 cut(s) 250, 435, 495, 1410, 1549, 2437
BstNI CCWGG 5 cut(s) 1307, 1766, 2019, 2193, 2637
BstNSI RCATGY 3 cut(s) 1673, 2308, 2651
BstSCI CCNGG 6 cut(s) 177, 1305, 1764, 2017, 2191, 2635
BstSLI GKGCMC 2 cut(s) 856, 1609
BstUI CGCG 3 cut(s) 258, 445, 2333
BstV2I GAAGAC 2 cut(s) 885, 1286
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BsuRI GGCC 5 cut(s) 478, 1526, 2022, 2417, 2643
BtgI CCRYGG 2 cut(s) 279, 1798
BtgZI GCGATG 1 cut(s) 2348
BtsCI GGATG 4 cut(s) 577, 1186, 2329, 2400
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 4 cut(s) 508, 665, 1607, 2067
BveI ACCTGC 3 cut(s) 1649, 1711, 1832
CaiI CAGNNNCTG 5 cut(s) 344, 1343, 2477, 2714, 2723
CciI TCATGA 1 cut(s) 1882
CfoI GCGC 6 cut(s) 260, 445, 1089, 2236, 2387, 2491
Cfr10I RCCGGY 2 cut(s) 1526, 2643
Cfr13I GGNCC 2 cut(s) 476, 708
CsiI ACCWGGT 1 cut(s) 2635
Csp6I GTAC 2 cut(s) 1726, 2329
CviQI GTAC 2 cut(s) 1726, 2329
DdeI CTNAG 5 cut(s) 323, 603, 1521, 2377, 2531
DpnI GATC 6 cut(s) 252, 437, 497, 1412, 1551, 2439
DpnII GATC 6 cut(s) 250, 435, 495, 1410, 1549, 2437
DraI TTTAAA 1 cut(s) 1561
EaeI YGGCCR 1 cut(s) 2641
Eam1104I CTCTTC 3 cut(s) 1455, 1494, 2622
EarI CTCTTC 3 cut(s) 1455, 1494, 2622
Ecl136II GAGCTC 1 cut(s) 1170
Eco130I CCWWGG 3 cut(s) 279, 1779, 1798
Eco147I AGGCCT 2 cut(s) 2022, 2417
Eco24I GRGCYC 2 cut(s) 1172, 1332
Eco32I GATATC 1 cut(s) 628
Eco47I GGWCC 1 cut(s) 708
Eco53kI GAGCTC 1 cut(s) 1170
Eco57I CTGAAG 6 cut(s) 299, 1284, 1419, 1479, 2154, 2606
EcoICRI GAGCTC 1 cut(s) 1170
EcoNI CCTNNNNNAGG 1 cut(s) 1884
EcoO109I RGGNCCY 1 cut(s) 708
EcoRI GAATTC 1 cut(s) 1012
EcoRII CCWGG 5 cut(s) 1305, 1764, 2017, 2191, 2635
EcoRV GATATC 1 cut(s) 628
EcoT14I CCWWGG 3 cut(s) 279, 1779, 1798
EcoT38I GRGCYC 2 cut(s) 1172, 1332
ErhI CCWWGG 3 cut(s) 279, 1779, 1798
FalI AAGNNNNNCTT 2 cut(s) 175, 207
FaqI GGGAC 3 cut(s) 721, 849, 2356
FauI CCCGC 5 cut(s) 1314, 1417, 2155, 2534, 2694
FblI GTMKAC 1 cut(s) 969
FokI GGATG 4 cut(s) 584, 1193, 2336, 2407
FriOI GRGCYC 2 cut(s) 1172, 1332
FspBI CTAG 6 cut(s) 30, 60, 773, 1242, 2388, 2751
FspI TGCGCA 1 cut(s) 1088
GlaI GCGC 6 cut(s) 259, 444, 1088, 2235, 2386, 2490
GsaI CCCAGC 1 cut(s) 708
GsuI CTGGAG 2 cut(s) 452, 1491
HaeII RGCGCY 1 cut(s) 2388
HaeIII GGCC 5 cut(s) 478, 1526, 2022, 2417, 2643
HapII CCGG 6 cut(s) 68, 178, 561, 1527, 2206, 2644
HhaI GCGC 6 cut(s) 260, 445, 1089, 2236, 2387, 2491
Hin1I GRCGYC 1 cut(s) 242
Hin6I GCGC 6 cut(s) 258, 443, 1087, 2234, 2385, 2489
HinP1I GCGC 6 cut(s) 258, 443, 1087, 2234, 2385, 2489
HincII GTYRAC 1 cut(s) 970
HindII GTYRAC 1 cut(s) 970
HindIII AAGCTT 4 cut(s) 184, 320, 548, 1539
HinfI GANTC 9 cut(s) 4, 341, 452, 690, 1066, 1483, 1921, 2176, 2767
HpaII CCGG 6 cut(s) 68, 178, 561, 1527, 2206, 2644
HphI GGTGA 2 cut(s) 1589, 2771
Hpy166II GTNNAC 6 cut(s) 240, 970, 1607, 1726, 2425, 2628
Hpy8I GTNNAC 6 cut(s) 240, 970, 1607, 1726, 2425, 2628
HpyCH4III ACNGT 5 cut(s) 142, 404, 644, 816, 1808
HpyCH4IV ACGT 4 cut(s) 242, 382, 1291, 2447
HpyF3I CTNAG 5 cut(s) 323, 603, 1521, 2377, 2531
HpySE526I ACGT 4 cut(s) 242, 382, 1291, 2447
Hsp92I GRCGYC 1 cut(s) 242
HspAI GCGC 6 cut(s) 258, 443, 1087, 2234, 2385, 2489
Kpn2I TCCGGA 3 cut(s) 67, 560, 2205
KroI GCCGGC 1 cut(s) 2643
KroNI GCCGGC 1 cut(s) 2645
Kzo9I GATC 6 cut(s) 250, 435, 495, 1410, 1549, 2437
LguI GCTCTTC 2 cut(s) 1494, 2622
LmnI GCTCC 3 cut(s) 898, 1335, 2684
MabI ACCWGGT 1 cut(s) 2635
MaeI CTAG 6 cut(s) 30, 60, 773, 1242, 2388, 2751
MaeII ACGT 4 cut(s) 242, 382, 1291, 2447
MaeIII GTNAC 5 cut(s) 173, 453, 918, 1691, 2494
MalI GATC 6 cut(s) 252, 437, 497, 1412, 1551, 2439
MboI GATC 6 cut(s) 250, 435, 495, 1410, 1549, 2437
MfeI CAATTG 2 cut(s) 115, 1953
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 9 cut(s) 856, 1172, 1332, 1506, 1609, 1849, 2047, 2170, 2509
MlyI GAGTC 2 cut(s) 13, 461
MmeI TCCRAC 3 cut(s) 1034, 1474, 1810
MroI TCCGGA 3 cut(s) 67, 560, 2205
MroNI GCCGGC 1 cut(s) 2643
MroXI GAANNNNTTC 1 cut(s) 1065
MseI TTAA 7 cut(s) 591, 1160, 1301, 1560, 1748, 2187, 2800
MslI CAYNNNNRTG 3 cut(s) 1160, 1509, 1887
MspA1I CMGCKG 5 cut(s) 1601, 1907, 2159, 2541, 2711
MspI CCGG 6 cut(s) 68, 178, 561, 1527, 2206, 2644
MspR9I CCNGG 6 cut(s) 179, 1307, 1766, 2019, 2193, 2637
MunI CAATTG 2 cut(s) 115, 1953
Mva1269I GAATGC 1 cut(s) 1378
MvaI CCWGG 5 cut(s) 1307, 1766, 2019, 2193, 2637
MvnI CGCG 3 cut(s) 258, 445, 2333
NaeI GCCGGC 1 cut(s) 2645
NciI CCSGG 1 cut(s) 179
NcoI CCATGG 2 cut(s) 279, 1798
NdeII GATC 6 cut(s) 250, 435, 495, 1410, 1549, 2437
NgoMIV GCCGGC 1 cut(s) 2643
NheI GCTAGC 1 cut(s) 1241
NlaIV GGNNCC 3 cut(s) 709, 1331, 1513
NmeAIII GCCGAG 1 cut(s) 1120
NmuCI GTSAC 3 cut(s) 173, 453, 2494
NsbI TGCGCA 1 cut(s) 1088
NspI RCATGY 3 cut(s) 1673, 2308, 2651
OliI CACNNNNGTG 1 cut(s) 1509
PaeI GCATGC 2 cut(s) 1673, 2651
PagI TCATGA 1 cut(s) 1882
PaqCI CACCTGC 3 cut(s) 1649, 1711, 1832
PceI AGGCCT 2 cut(s) 2022, 2417
PciI ACATGT 1 cut(s) 2304
PciSI GCTCTTC 2 cut(s) 1494, 2622
PctI GAATGC 1 cut(s) 1378
PdiI GCCGGC 1 cut(s) 2645
PdmI GAANNNNTTC 1 cut(s) 1065
PfeI GAWTC 7 cut(s) 341, 690, 1066, 1483, 1921, 2176, 2767
PleI GAGTC 2 cut(s) 12, 460
PpsI GAGTC 2 cut(s) 12, 460
Ppu21I YACGTR 1 cut(s) 383
PpuMI RGGWCCY 1 cut(s) 708
PscI ACATGT 1 cut(s) 2304
PshBI ATTAAT 2 cut(s) 591, 1748
Psp124BI GAGCTC 1 cut(s) 1172
Psp1406I AACGTT 1 cut(s) 2447
Psp5II RGGWCCY 1 cut(s) 708
Psp6I CCWGG 5 cut(s) 1305, 1764, 2017, 2191, 2635
PspFI CCCAGC 1 cut(s) 704
PspGI CCWGG 5 cut(s) 1305, 1764, 2017, 2191, 2635
PspN4I GGNNCC 3 cut(s) 709, 1331, 1513
PspPI GGNCC 2 cut(s) 476, 708
PspPPI RGGWCCY 1 cut(s) 708
PstI CTGCAG 7 cut(s) 1828, 1963, 2143, 2464, 2482, 2710, 2719
PstNI CAGNNNCTG 5 cut(s) 344, 1343, 2477, 2714, 2723
PsuI RGATCY 1 cut(s) 250
PvuII CAGCTG 4 cut(s) 1601, 1907, 2159, 2711
RsaI GTAC 2 cut(s) 1727, 2330
RsaNI GTAC 2 cut(s) 1726, 2329
RseI CAYNNNNRTG 3 cut(s) 1160, 1509, 1887
SacI GAGCTC 1 cut(s) 1172
SalI GTCGAC 1 cut(s) 968
SapI GCTCTTC 2 cut(s) 1494, 2622
SaqAI TTAA 7 cut(s) 591, 1160, 1301, 1560, 1748, 2187, 2800
Sau3AI GATC 6 cut(s) 250, 435, 495, 1410, 1549, 2437
Sau96I GGNCC 2 cut(s) 476, 708
SchI GAGTC 2 cut(s) 13, 461
ScrFI CCNGG 6 cut(s) 179, 1307, 1766, 2019, 2193, 2637
SduI GDGCHC 9 cut(s) 856, 1172, 1332, 1506, 1609, 1849, 2047, 2170, 2509
SexAI ACCWGGT 1 cut(s) 2635
SinI GGWCC 1 cut(s) 708
SmiMI CAYNNNNRTG 3 cut(s) 1160, 1509, 1887
SmlI CTYRAG 3 cut(s) 1130, 2594, 2771
SmoI CTYRAG 3 cut(s) 1130, 2594, 2771
SphI GCATGC 2 cut(s) 1673, 2651
SseBI AGGCCT 2 cut(s) 2022, 2417
SsiI CCGC 8 cut(s) 16, 445, 1321, 1424, 1968, 2162, 2541, 2701
SspI AATATT 2 cut(s) 357, 1417
SspMI CTAG 6 cut(s) 30, 60, 773, 1242, 2388, 2751
SstI GAGCTC 1 cut(s) 1172
StuI AGGCCT 2 cut(s) 2022, 2417
StyD4I CCNGG 6 cut(s) 177, 1305, 1764, 2017, 2191, 2635
StyI CCWWGG 3 cut(s) 279, 1779, 1798
TaaI ACNGT 5 cut(s) 142, 404, 644, 816, 1808
TaiI ACGT 4 cut(s) 245, 385, 1294, 2450
TaqI TCGA 4 cut(s) 553, 688, 969, 1945
TatI WGTACW 1 cut(s) 1725
TauI GCSGC 2 cut(s) 19, 448
TfiI GAWTC 7 cut(s) 341, 690, 1066, 1483, 1921, 2176, 2767
Tru1I TTAA 7 cut(s) 591, 1160, 1301, 1560, 1748, 2187, 2800
Tru9I TTAA 7 cut(s) 591, 1160, 1301, 1560, 1748, 2187, 2800
TscAI CASTG 4 cut(s) 508, 672, 1614, 2067
TseFI GTSAC 3 cut(s) 173, 453, 2494
Tsp45I GTSAC 3 cut(s) 173, 453, 2494
TspGWI ACGGA 1 cut(s) 2590
TspRI CASTG 4 cut(s) 508, 672, 1614, 2067
VneI GTGCAC 1 cut(s) 1605
VpaK11BI GGWCC 1 cut(s) 708
VspI ATTAAT 2 cut(s) 591, 1748
XagI CCTNNNNNAGG 1 cut(s) 1884
XapI RAATTY 6 cut(s) 222, 376, 616, 1012, 1615, 1786
XceI RCATGY 3 cut(s) 1673, 2308, 2651
XmiI GTMKAC 1 cut(s) 969
XmnI GAANNNNTTC 1 cut(s) 1065
XspI CTAG 6 cut(s) 30, 60, 773, 1242, 2388, 2751
ZraI GACGTC 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.