Rorug01G0186800

ARABIDILLO 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0010465
Physical Location & Seq
Forward (+)
25830807 .. 25833995
3189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0186800.1

Sequence Viewer

Length: 1302 bp
ATGAAGCAACTCACATTCTCACTCCAATCATCCCTTCTACTAATATCTCTTATATTTGTCACAACCTCAGTTTCCTTAAAAATTCCAAGGCTGAGTCCAACTGGAGGGCATTGGACACAAGGCCGACCCAGCGAAGCTTCATCATCTGCATTTGATCCCAAAGATCTTCAGACATTTTACTACAACCAAACACTTGACCATTTCAACTTTAGGTATGATAGCTTCAATACTTTTCAACAAAGATATTTGATCAATTCCAAGCATTGGGGTGGCTCCAATATCAGTGCACCTATACTCGCTTACTTAGGCGCAGAAGAATCAATTGATGTTGATATACCTATTATTGGGTTCCTACCTGAAAACGCCATTCACTTTCAAGCCCTCCAGATATATATAGAGCACCGGTACTATGGGGAATCAATCCCATTCGGATCAACGGAAGTGGCATTTAAAAATGCAAGCACTCTTGGATACTTCAACTCAGCCCAAGCTATAGCAGATTATGCAGAAATTCTCATACATGTAAAGAAGCAGCTACATGCTGAACATTCTCCGGTCATTGTTATTGGTGGCTCATATGGTGGAATGCTTGCTTCATGGTTTCGTCTAAAGTATCCCCACGTCGCTCTTGGAGCTCTGGCCTCATCAGCTCCAATTCTATACTTTGATGACATAGTTCCTCCAGAAACAGGATACTACTCCATTGTCACCAAGGATTTTCGAGAAGCTAGTGAGACTTGCTACCAAATCATAAAGAAGTCTTGGTCTGAAATTGATATTATTGCTTCTAAGCCTGAAGGCCTCTCAAGTCTTAGCAAGAAATTCCGAACTTGCAGACCGTTGACCACGTCGTCTGAGCTCAAGGACTACTTGGACTCGATGTATGCTGGGGCAGCTCAATACGATCGTCCACCGAATTATCCGGTTACTGTAGTCTGCGGTGGCATTGATGGAGCTTCATCTACAGATGATATTTTGACAAAAATATTTGCAGGTGTGGTTGCATATACCGGAAACCTGTCATGCTATGTCAACCAGCCCAGAAATGTAACAGAAAGACACGTCGGGTGGGCCTGGCAGACATGTAGTGACATGGTGATTCCCATAAGCGTGAACAACGACACCATGTTTCCACCGTACAAATTTGATCTCGATGAATTTATCAATAACTGCAAGGCAACCTATGGCGTCCCTCCCAGGCCTAATTGGGTGACTACCTACTTTGGAGGCCATGACATAAAACTGAGTCTCTCCAGGTTTGCTAGCAACATCATTTTCTCCAATGGGCTAAGAGACCCTTAA

Protein Analysis

433

Amino Acids

48.33

Weight (kDa)

6.0

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 63 - 433 1.1e-61 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 983
AasI GACNNNNNNGTC 1 cut(s) 850
Acc36I ACCTGC 1 cut(s) 983
AccB7I CCANNNNNTGG 1 cut(s) 264
AciI CCGC 1 cut(s) 939
AclWI GGATC 2 cut(s) 149, 439
AcsI RAATTY 5 cut(s) 81, 510, 821, 1142, 1157
AcuI CTGAAG 2 cut(s) 152, 816
AcyI GRCGYC 1 cut(s) 1188
AfaI GTAC 2 cut(s) 407, 1139
AfiI CCNNNNNNNGG 4 cut(s) 104, 264, 344, 689
AflIII ACRYGT 3 cut(s) 520, 1060, 1082
AgeI ACCGGT 1 cut(s) 402
AgsI TTSAA 5 cut(s) 205, 226, 236, 377, 478
AjiI CACGTC 3 cut(s) 622, 849, 1063
AjnI CCWGG 3 cut(s) 1073, 1196, 1253
AjuI GAANNNNNNNTTGG 2 cut(s) 18, 50
Alw21I GWGCWC 4 cut(s) 289, 402, 637, 861
Alw26I GTCTC 3 cut(s) 728, 1253, 1287
Alw44I GTGCAC 1 cut(s) 285
AlwI GGATC 2 cut(s) 149, 439
AoxI GGCC 6 cut(s) 121, 639, 799, 1071, 1199, 1228
ApaLI GTGCAC 1 cut(s) 285
ApeKI GCWGC 2 cut(s) 532, 893
ApoI RAATTY 5 cut(s) 81, 510, 821, 1142, 1157
AsiGI ACCGGT 1 cut(s) 402
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 1 cut(s) 1071
AsuHPI GGTGA 3 cut(s) 700, 1108, 1222
AsuNHI GCTAGC 1 cut(s) 1262
BaeGI GKGCMC 1 cut(s) 289
BanII GRGCYC 2 cut(s) 637, 861
BarI GAAGNNNNNNTAC 2 cut(s) 206, 238
Bbv12I GWGCWC 4 cut(s) 289, 402, 637, 861
BbvI GCAGC 2 cut(s) 544, 905
BccI CCATC 1 cut(s) 944
BciT130I CCWGG 3 cut(s) 1075, 1198, 1255
BciVI GTATCC 3 cut(s) 464, 624, 686
BclI TGATCA 1 cut(s) 249
BcoDI GTCTC 3 cut(s) 728, 1253, 1287
BfaI CTAG 2 cut(s) 729, 1263
BfmI CTRYAG 3 cut(s) 492, 930, 963
BfuAI ACCTGC 1 cut(s) 983
BfuI GTATCC 3 cut(s) 464, 624, 686
BglII AGATCT 1 cut(s) 163
BisI GCNGC 2 cut(s) 533, 894
BlsI GCNGC 2 cut(s) 534, 895
Bme1390I CCNGG 3 cut(s) 1075, 1198, 1255
BmgBI CACGTC 3 cut(s) 622, 849, 1063
BmgT120I GGNCC 1 cut(s) 1071
BmiI GGNNCC 2 cut(s) 274, 350
BmrFI CCNGG 3 cut(s) 1075, 1198, 1255
BmtI GCTAGC 1 cut(s) 1266
BpmI CTGGAG 4 cut(s) 123, 368, 666, 1237
BpuEI CTTGAG 2 cut(s) 790, 845
BsaHI GRCGYC 1 cut(s) 1188
BsaI GGTCTC 1 cut(s) 1287
BsaJI CCNNGG 3 cut(s) 86, 711, 1196
BsaWI WCCGGW 4 cut(s) 402, 553, 922, 1010
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 264, 344, 689
Bse118I RCCGGY 1 cut(s) 402
Bse1I ACTGG 1 cut(s) 106
BseBI CCWGG 3 cut(s) 1075, 1198, 1255
BseDI CCNNGG 3 cut(s) 86, 711, 1196
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 4 cut(s) 104, 264, 344, 689
BseMII CTCAG 5 cut(s) 81, 83, 495, 846, 1235
BseNI ACTGG 1 cut(s) 106
BseSI GKGCMC 1 cut(s) 289
BseXI GCAGC 2 cut(s) 544, 905
BseYI CCCAGC 2 cut(s) 128, 887
Bsh1285I CGRYCG 1 cut(s) 907
BshFI GGCC 6 cut(s) 123, 641, 801, 1073, 1201, 1230
BshTI ACCGGT 1 cut(s) 402
BsiEI CGRYCG 1 cut(s) 907
BsiHKAI GWGCWC 4 cut(s) 289, 402, 637, 861
BsiSI CCGG 4 cut(s) 403, 554, 923, 1011
BslFI GGGAC 1 cut(s) 1175
BslI CCNNNNNNNGG 4 cut(s) 104, 264, 344, 689
BsmAI GTCTC 3 cut(s) 728, 1253, 1287
BsmFI GGGAC 1 cut(s) 1175
BsmI GAATGC 1 cut(s) 591
BsnI GGCC 6 cut(s) 123, 641, 801, 1073, 1201, 1230
Bso31I GGTCTC 1 cut(s) 1287
Bsp1286I GDGCHC 4 cut(s) 289, 402, 637, 861
Bsp143I GATC 6 cut(s) 154, 163, 249, 431, 904, 1147
BspACI CCGC 1 cut(s) 939
BspANI GGCC 6 cut(s) 123, 641, 801, 1073, 1201, 1230
BspCNI CTCAG 5 cut(s) 80, 84, 494, 847, 1236
BspLI GGNNCC 2 cut(s) 274, 350
BspMI ACCTGC 1 cut(s) 983
BspOI GCTAGC 1 cut(s) 1266
BspPI GGATC 2 cut(s) 149, 439
BspTNI GGTCTC 1 cut(s) 1287
BsrFI RCCGGY 1 cut(s) 402
BsrI ACTGG 1 cut(s) 106
BssAI RCCGGY 1 cut(s) 402
BssECI CCNNGG 3 cut(s) 86, 711, 1196
BssMI GATC 6 cut(s) 154, 163, 249, 431, 904, 1147
BssNI GRCGYC 1 cut(s) 1188
BssT1I CCWWGG 2 cut(s) 86, 711
Bst2UI CCWGG 3 cut(s) 1075, 1198, 1255
Bst4CI ACNGT 3 cut(s) 840, 931, 1137
BstACI GRCGYC 1 cut(s) 1188
BstAPI GCANNNNNTGC 1 cut(s) 503
BstC8I GCNNGC 3 cut(s) 460, 591, 1264
BstDEI CTNAG 9 cut(s) 67, 92, 304, 481, 789, 812, 855, 1244, 1289
BstF5I GGATG 1 cut(s) 29
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 6 cut(s) 157, 166, 252, 434, 907, 1150
BstMAI GTCTC 3 cut(s) 728, 1253, 1287
BstMBI GATC 6 cut(s) 154, 163, 249, 431, 904, 1147
BstMCI CGRYCG 1 cut(s) 907
BstMWI GCNNNNNNNGC 5 cut(s) 129, 503, 632, 647, 893
BstNI CCWGG 3 cut(s) 1075, 1198, 1255
BstNSI RCATGY 3 cut(s) 524, 542, 1086
BstSCI CCNGG 3 cut(s) 1073, 1196, 1253
BstSFI CTRYAG 3 cut(s) 492, 930, 963
BstSLI GKGCMC 1 cut(s) 289
BstV1I GCAGC 2 cut(s) 544, 905
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
BsuI GTATCC 3 cut(s) 464, 624, 686
BsuRI GGCC 6 cut(s) 123, 641, 801, 1073, 1201, 1230
BtrI CACGTC 3 cut(s) 622, 849, 1063
BtsCI GGATG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 289
BveI ACCTGC 1 cut(s) 983
Cac8I GCNNGC 3 cut(s) 460, 591, 1264
CfoI GCGC 1 cut(s) 311
Cfr10I RCCGGY 1 cut(s) 402
Cfr13I GGNCC 1 cut(s) 1071
CseI GACGC 1 cut(s) 1177
Csp6I GTAC 2 cut(s) 406, 1138
CspAI ACCGGT 1 cut(s) 402
CspCI CAANNNNNGTGG 2 cut(s) 423, 458
CviAII CATG 8 cut(s) 521, 539, 597, 1023, 1083, 1093, 1126, 1232
CviQI GTAC 2 cut(s) 406, 1138
DdeI CTNAG 9 cut(s) 67, 92, 304, 481, 789, 812, 855, 1244, 1289
DpnI GATC 6 cut(s) 156, 165, 251, 433, 906, 1149
DpnII GATC 6 cut(s) 154, 163, 249, 431, 904, 1147
DraI TTTAAA 1 cut(s) 451
DrdI GACNNNNNNGTC 1 cut(s) 850
DseDI GACNNNNNNGTC 1 cut(s) 850
Ecl136II GAGCTC 2 cut(s) 635, 859
Eco130I CCWWGG 2 cut(s) 86, 711
Eco147I AGGCCT 2 cut(s) 801, 1201
Eco24I GRGCYC 2 cut(s) 637, 861
Eco31I GGTCTC 1 cut(s) 1287
Eco53kI GAGCTC 2 cut(s) 635, 859
Eco57I CTGAAG 2 cut(s) 152, 816
EcoICRI GAGCTC 2 cut(s) 635, 859
EcoRII CCWGG 3 cut(s) 1073, 1196, 1253
EcoT14I CCWWGG 2 cut(s) 86, 711
EcoT38I GRGCYC 2 cut(s) 637, 861
ErhI CCWWGG 2 cut(s) 86, 711
FaeI CATG 8 cut(s) 524, 542, 600, 1026, 1086, 1096, 1129, 1235
FalI AAGNNNNNCTT 3 cut(s) 854, 886, 1282
FaqI GGGAC 1 cut(s) 1175
FatI CATG 8 cut(s) 520, 538, 596, 1022, 1082, 1092, 1125, 1231
FauNDI CATATG 1 cut(s) 577
FbaI TGATCA 1 cut(s) 249
Fnu4HI GCNGC 2 cut(s) 533, 894
FokI GGATG 1 cut(s) 16
FriOI GRGCYC 2 cut(s) 637, 861
Fsp4HI GCNGC 2 cut(s) 533, 894
FspBI CTAG 2 cut(s) 729, 1263
GlaI GCGC 1 cut(s) 310
GluI GCNGC 2 cut(s) 533, 894
GsaI CCCAGC 2 cut(s) 132, 891
GsuI CTGGAG 4 cut(s) 123, 368, 666, 1237
HaeIII GGCC 6 cut(s) 123, 641, 801, 1073, 1201, 1230
HapII CCGG 4 cut(s) 403, 554, 923, 1011
HgaI GACGC 1 cut(s) 1177
HhaI GCGC 1 cut(s) 311
Hin1I GRCGYC 1 cut(s) 1188
Hin1II CATG 8 cut(s) 524, 542, 600, 1026, 1086, 1096, 1129, 1235
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HincII GTYRAC 2 cut(s) 843, 1033
HindII GTYRAC 2 cut(s) 843, 1033
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 6 cut(s) 94, 317, 416, 875, 1099, 1246
HpaII CCGG 4 cut(s) 403, 554, 923, 1011
HphI GGTGA 3 cut(s) 700, 1108, 1222
Hpy166II GTNNAC 5 cut(s) 287, 843, 911, 1033, 1114
Hpy188I TCNGA 5 cut(s) 171, 431, 769, 827, 856
Hpy188III TCNNGA 4 cut(s) 385, 683, 722, 1151
Hpy8I GTNNAC 5 cut(s) 287, 843, 911, 1033, 1114
Hpy99I CGWCG 3 cut(s) 626, 853, 1067
HpyAV CCTTC 2 cut(s) 44, 791
HpyCH4III ACNGT 3 cut(s) 840, 931, 1137
HpyCH4IV ACGT 3 cut(s) 621, 848, 1062
HpyCH4V TGCA 8 cut(s) 149, 287, 458, 506, 834, 992, 1004, 1173
HpyF10VI GCNNNNNNNGC 5 cut(s) 129, 503, 632, 647, 893
HpyF3I CTNAG 9 cut(s) 67, 92, 304, 481, 789, 812, 855, 1244, 1289
HpySE526I ACGT 3 cut(s) 621, 848, 1062
Hsp92I GRCGYC 1 cut(s) 1188
Hsp92II CATG 8 cut(s) 524, 542, 600, 1026, 1086, 1096, 1129, 1235
HspAI GCGC 1 cut(s) 309
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 6 cut(s) 154, 163, 249, 431, 904, 1147
LmnI GCTCC 4 cut(s) 278, 632, 655, 953
Lsp1109I GCAGC 2 cut(s) 544, 905
MaeI CTAG 2 cut(s) 729, 1263
MaeII ACGT 3 cut(s) 621, 848, 1062
MaeIII GTNAC 6 cut(s) 58, 706, 925, 1048, 1088, 1210
MalI GATC 6 cut(s) 156, 165, 251, 433, 906, 1149
MboI GATC 6 cut(s) 154, 163, 249, 431, 904, 1147
MboII GAAGA 2 cut(s) 158, 326
MfeI CAATTG 1 cut(s) 321
MflI RGATCY 1 cut(s) 163
MhlI GDGCHC 4 cut(s) 289, 402, 637, 861
MlyI GAGTC 3 cut(s) 103, 869, 1255
MmeI TCCRAC 1 cut(s) 122
MnlI CCTC 8 cut(s) 76, 98, 392, 652, 690, 812, 1203, 1220
MseI TTAA 3 cut(s) 77, 450, 1300
MslI CAYNNNNRTG 2 cut(s) 267, 1109
MspI CCGG 4 cut(s) 403, 554, 923, 1011
MspR9I CCNGG 3 cut(s) 1075, 1198, 1255
MunI CAATTG 1 cut(s) 321
Mva1269I GAATGC 1 cut(s) 591
MvaI CCWGG 3 cut(s) 1075, 1198, 1255
MwoI GCNNNNNNNGC 5 cut(s) 129, 503, 632, 647, 893
NdeI CATATG 1 cut(s) 577
NdeII GATC 6 cut(s) 154, 163, 249, 431, 904, 1147
NheI GCTAGC 1 cut(s) 1262
NlaIII CATG 8 cut(s) 524, 542, 600, 1026, 1086, 1096, 1129, 1235
NlaIV GGNNCC 2 cut(s) 274, 350
NmuCI GTSAC 4 cut(s) 58, 706, 1088, 1210
NspI RCATGY 3 cut(s) 524, 542, 1086
PaqCI CACCTGC 1 cut(s) 983
PceI AGGCCT 2 cut(s) 801, 1201
PciI ACATGT 2 cut(s) 520, 1082
PctI GAATGC 1 cut(s) 591
PfeI GAWTC 3 cut(s) 317, 416, 1099
PflFI GACNNNGTC 1 cut(s) 847
PflMI CCANNNNNTGG 1 cut(s) 264
PinAI ACCGGT 1 cut(s) 402
PkrI GCNGC 2 cut(s) 534, 895
Ple19I CGATCG 1 cut(s) 907
PleI GAGTC 3 cut(s) 102, 869, 1254
PpsI GAGTC 3 cut(s) 102, 869, 1254
PscI ACATGT 2 cut(s) 520, 1082
Psp124BI GAGCTC 2 cut(s) 637, 861
Psp6I CCWGG 3 cut(s) 1073, 1196, 1253
PspFI CCCAGC 2 cut(s) 128, 887
PspGI CCWGG 3 cut(s) 1073, 1196, 1253
PspN4I GGNNCC 2 cut(s) 274, 350
PspPI GGNCC 1 cut(s) 1071
PsuI RGATCY 1 cut(s) 163
PsyI GACNNNGTC 1 cut(s) 847
PvuI CGATCG 1 cut(s) 907
RsaI GTAC 2 cut(s) 407, 1139
RsaNI GTAC 2 cut(s) 406, 1138
RseI CAYNNNNRTG 2 cut(s) 267, 1109
SacI GAGCTC 2 cut(s) 637, 861
SaqAI TTAA 3 cut(s) 77, 450, 1300
SatI GCNGC 2 cut(s) 533, 894
Sau3AI GATC 6 cut(s) 154, 163, 249, 431, 904, 1147
Sau96I GGNCC 1 cut(s) 1071
SchI GAGTC 3 cut(s) 103, 869, 1255
ScrFI CCNGG 3 cut(s) 1075, 1198, 1255
SduI GDGCHC 4 cut(s) 289, 402, 637, 861
SfcI CTRYAG 3 cut(s) 492, 930, 963
SmiMI CAYNNNNRTG 2 cut(s) 267, 1109
SmlI CTYRAG 2 cut(s) 805, 860
SmoI CTYRAG 2 cut(s) 805, 860
SseBI AGGCCT 2 cut(s) 801, 1201
SsiI CCGC 1 cut(s) 939
SspI AATATT 1 cut(s) 987
SspMI CTAG 2 cut(s) 729, 1263
SstI GAGCTC 2 cut(s) 637, 861
StuI AGGCCT 2 cut(s) 801, 1201
StyD4I CCNGG 3 cut(s) 1073, 1196, 1253
StyI CCWWGG 2 cut(s) 86, 711
TaaI ACNGT 3 cut(s) 840, 931, 1137
TaiI ACGT 3 cut(s) 624, 851, 1065
TaqI TCGA 3 cut(s) 721, 878, 1152
TfiI GAWTC 3 cut(s) 317, 416, 1099
Tru1I TTAA 3 cut(s) 77, 450, 1300
Tru9I TTAA 3 cut(s) 77, 450, 1300
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 4 cut(s) 58, 706, 1088, 1210
TseI GCWGC 2 cut(s) 532, 893
Tsp45I GTSAC 4 cut(s) 58, 706, 1088, 1210
TspDTI ATGAA 5 cut(s) 17, 129, 585, 948, 1170
TspGWI ACGGA 1 cut(s) 452
TspRI CASTG 1 cut(s) 289
Tth111I GACNNNGTC 1 cut(s) 847
Van91I CCANNNNNTGG 1 cut(s) 264
VneI GTGCAC 1 cut(s) 285
XapI RAATTY 5 cut(s) 81, 510, 821, 1142, 1157
XceI RCATGY 3 cut(s) 524, 542, 1086
XcmI CCANNNNNNNNNTGG 1 cut(s) 626
XspI CTAG 2 cut(s) 729, 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.