Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0010993

Physical Location & Seq

Genomic Coordinates Forward (+)

15076441 .. 15080220

Gene Structure 3780 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1120700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2049 bp

ATGCATTATTTCCTTCTCTCCCTTCTCCTCCCCCTCCTCCTCCTCCTCCCCCTCTCTATCTCCTCGCTCACCGAGCTCCCTGCTCTCATTGCAATGAAGGCCTCTCTGGACCCACAAAACCAGTTCTTGACGTCATGGACGCCGCACGCGGACCCCTGCGGCGGCGCTTTTGAAGGCGTGGCTTGCAACGAGCAGGGCCGTGTGACTAATATCTCTCTGCAAGGGAAGGGTCTTTGGGGCCAGATTCCCCCAGCTGTTGGCGGCCTGAAGAACCTCACTGGGCTGTACCTTCACTTCAATGCTTTGACTGGGAAGATCCCAAAGCAGATTGCTGCTTTGAACCAGCTCGCCGATTTGTACCTCAATGTGAACAATCTGTCAGGTGAAATTCCTCGTGAGATTGGTAAAATGCCCAATTTGCAAGTTTTGCAGTTGTGTTACAACAAGTTGACTGGGGGAATACCTACAGAATTAGGGCAGCTTAAAAGACTTAGTGTTCTTGCAGTGCAATCCAATGAGTTAACGGGGGCAATTCCTGCAAGTTTGGGTGAGTTGGGGACGCTAACAAGGTTGGATTTGAGCTTTAATAGCTTTTTTGGTCCTATTCCAGCAAAATTAGCTGATGCTTCTATGCTAGAAGTCCTTGACGTTCGAAGCAACACTCTCTCAGGAAATATACCTCCAGCTCTAAAGAGACTTAATGAAGGATTCCAATACGAAAACAACCCGAACTTATGTGGGGTTGGGTTTTCTGGTTTAGAACCGTGCACAGCTACTACTCGGAACCCAAACATGCCACAACCATTTGAACCTAGTAACTTTTCTGGAAAAGGTCTTCCAGACTCATCTACTGCAAAAGAAATCCCCGAGACAGCAAATATTCATTCCAATTGTAGCCAAACCCAGTGTTCAGAAGCATCAAACTCCCCAGAAATTGGTTTTGTCTTTGGGGTGATCGGAGCTGTTGTTGCTTTAGCTGTATCTGGGCTCTTCTTGTTCTCCTGGTGTCGTCGACAAAAGCAGAAGATTGGGAGCACTTTTGACTCTTCTGATAGCAGGCTTAGTACTGGCCAAGCAAAGGCAGTTTACAAGAAGAGTGTGTCTCCTCTCATCAATCTGGAGTATTCTAACGGGTGGGATCCATTGGCTAAAGGCAGCAGTGGGTACTCTCAAGAAGTTCTTGAGAGTTTTATGATAAATTTAGAAGAAGTGGAGCGTGCAACTCAAAGCTTTTTGGAAGTCAATCTGTTGCGCAAGGGTAAATTTTCTGCCATCTATAAGGGGATCCTAAGAGACGGATCAGTTGTTACTATAAACTGCATTAGCAAGACAAGCTGTAAGCCCGACGAAGCTGAATTTTTGAAGGGGTTGAAGATATTGACATCATTAAAACATGAGAATCTTGTTAGGTTGAGAGGCTTTTGCTGCTCAAAAGGCAGGGGAGAGTGGTTTCTTATCTATGATTTTATCCCGAATGAAAGTCTGTTACAGTATCTCGACATTAAGGATGGCAGTGCTGAGGCTCTTGAATGGTCAACCAGAGTATCTATAATCAATGGGATCGCTAAAGGTATTGGATATTTGCATGGAAATGCAGACAATAAACCTGCTATAGTTCATCAGACCATATCAGCTGAGAAGGTGCTTATTGACTCCCACTATAACCCCTTACTATCGGATTCAGGTTTGCACAAACTCCTAGCAGATGATATTGTCTTCTCCATGCTCAAAGCCAGTGCTGCCATGGGGTACCTGGCTCCCGAGTACACCACCATTGGTCGTTTCACTTCAAAGAGTGACATTTATGCATTTGGTATGATTGTGTTTCAACTCCTTTGTGGAAAACGTAAAATCACCCAGGTGACTCGCCAAGGTGCTGAGGCCGGCAGATTTGAAGATTTTATTGATGCAAACCTGGAGGGAAAGTTTTCGGAATCAGAGGCAAGTAAACTTGGAAAACTTGCTCTTCTTTGCACGCACGAGTCTCCCATTTACAGGCCACCAATAGAAAATGTGGTCAAAGAGCTAAGCGAGTTCAATTGCACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

683

Amino Acids

74.16

Weight (kDa)

6.41

Isoelectric Point (pI)

34.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	24 - 63	2e-07	Leucine rich repeat N-terminal domain
LRR_14	PF23598	80 - 195	3.1e-10	Leucine-rich repeat region
LRR_8	PF13855	138 - 197	2.2e-06	Leucine rich repeat
PK_Tyr_Ser-Thr	PF07714	417 - 676	5.6e-32	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	417 - 674	5.7e-23	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010993)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45340
fragaria_vesca	FvH4_7g11610 FvH4_7g11610 FvH4_7g11610
malus_domestica	MD02G1205600.v1.1 MD07G1120700.v1.1
prunus_persica	Prupe.2G144000_v2.0.a1
pyrus_communis	pycom02g16740 pycom07g10460
rosa_chinensis	RchiOBHm_Chr1g0350041
rosa_laevigata	RLG00000028504
rosa_multiflora	Rmu_sc0003674.1_g000018
rosa_roxburghii	Rroxscaffold_4G00305100
rosa_rugosa	Rorug01G0208200
rosa_samantha	Rh1AG224500 Rh1BG191800 Rh1CG209200 Rh1DG220300
rosa_wichuraiana	Rw1G019180 Rw1G019300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	134
Acc16I	TGCGCA	1 cut(s)	1253
Acc36I	ACCTGC	1 cut(s)	1615
Acc65I	GGTACC	1 cut(s)	1749
AccB1I	GGYRCC	1 cut(s)	1749
AccB7I	CCANNNNNTGG	2 cut(s)	257, 935
AccI	GTMKAC	1 cut(s)	1012
AccII	CGCG	1 cut(s)	149
AciI	CCGC	5 cut(s)	143, 149, 159, 162, 261
AclWI	GGATC	7 cut(s)	310, 1133, 1146, 1279, 1292, 1306, 1568
AcoI	YGGCCR	1 cut(s)	1069
AcsI	RAATTY	4 cut(s)	387, 1198, 1262, 1355
AcuI	CTGAAG	1 cut(s)	287
AcyI	GRCGYC	2 cut(s)	131, 140
AfaI	GTAC	6 cut(s)	287, 359, 1066, 1166, 1751, 1766
AfiI	CCNNNNNNNGG	5 cut(s)	161, 257, 935, 1078, 1995
AjnI	CCWGG	4 cut(s)	1001, 1752, 1857, 1914
AleI	CACNNNNGTG	1 cut(s)	1859
Alw21I	GWGCWC	3 cut(s)	78, 770, 1037
Alw26I	GTCTC	5 cut(s)	688, 863, 1107, 1287, 1989
Alw44I	GTGCAC	1 cut(s)	766
AlwI	GGATC	7 cut(s)	310, 1133, 1146, 1279, 1292, 1306, 1568
Ama87I	CYCGRG	2 cut(s)	866, 1760
AoxI	GGCC	7 cut(s)	99, 196, 238, 262, 1069, 1881, 1997
ApaLI	GTGCAC	1 cut(s)	766
ApeKI	GCWGC	5 cut(s)	332, 478, 1155, 1425, 1739
ApoI	RAATTY	4 cut(s)	387, 1198, 1262, 1355
Asp700I	GAANNNNTTC	1 cut(s)	1927
Asp718I	GGTACC	1 cut(s)	1749
AspLEI	GCGC	2 cut(s)	167, 1254
AspS9I	GGNCC	5 cut(s)	109, 151, 196, 238, 599
AsuHPI	GGTGA	6 cut(s)	61, 395, 560, 964, 1846, 1873
AsuII	TTCGAA	1 cut(s)	652
AvaI	CYCGRG	2 cut(s)	866, 1760
AvaII	GGWCC	3 cut(s)	109, 151, 599
BaeGI	GKGCMC	1 cut(s)	770
BaeI	ACNNNNGTAYC	2 cut(s)	1527, 1560
BalI	TGGCCA	1 cut(s)	1071
BamHI	GGATCC	2 cut(s)	1138, 1284
BanI	GGYRCC	1 cut(s)	1749
BanII	GRGCYC	2 cut(s)	78, 990
BarI	GAAGNNNNNNTAC	2 cut(s)	278, 310
BauI	CACGAG	2 cut(s)	393, 1979
BbsI	GAAGAC	2 cut(s)	827, 1708
Bbv12I	GWGCWC	3 cut(s)	78, 770, 1037
BbvCI	CCTCAGC	2 cut(s)	1518, 1878
BbvI	GCAGC	5 cut(s)	319, 490, 1167, 1412, 1726
BccI	CCATC	2 cut(s)	1280, 1502
BceAI	ACGGC	1 cut(s)	183
BcgI	CGANNNNNNTGC	2 cut(s)	62, 96
BciT130I	CCWGG	4 cut(s)	1003, 1754, 1859, 1916
BcoDI	GTCTC	5 cut(s)	688, 863, 1107, 1287, 1989
BfaI	CTAG	3 cut(s)	635, 813, 1700
BfmI	CTRYAG	2 cut(s)	465, 1611
BfoI	RGCGCY	1 cut(s)	168
BfuAI	ACCTGC	1 cut(s)	1615
BisI	GCNGC	9 cut(s)	143, 160, 163, 262, 333, 479, 1156, 1426, 1740
BlpI	GCTNAGC	1 cut(s)	2027
BlsI	GCNGC	9 cut(s)	144, 161, 164, 263, 334, 480, 1157, 1427, 1741
BmcAI	AGTACT	1 cut(s)	1066
Bme1390I	CCNGG	4 cut(s)	1003, 1754, 1859, 1916
Bme18I	GGWCC	3 cut(s)	109, 151, 599
BmeT110I	CYCGRG	2 cut(s)	866, 1760
BmgT120I	GGNCC	5 cut(s)	109, 151, 196, 238, 599
BmiI	GGNNCC	8 cut(s)	111, 153, 239, 785, 1140, 1286, 1751, 1758
BmrFI	CCNGG	4 cut(s)	1003, 1754, 1859, 1916
BmrI	ACTGGG	4 cut(s)	288, 318, 462, 898
BmsI	GCATC	3 cut(s)	613, 926, 1897
BmuI	ACTGGG	4 cut(s)	288, 318, 462, 898
BpiI	GAAGAC	2 cut(s)	827, 1708
BplI	GAGNNNNNCTC	2 cut(s)	1087, 1119
BpmI	CTGGAG	3 cut(s)	666, 1139, 1937
Bpu10I	CCTNAGC	2 cut(s)	1518, 1878
Bpu1102I	GCTNAGC	1 cut(s)	2027
Bpu14I	TTCGAA	1 cut(s)	652
BpuEI	CTTGAG	2 cut(s)	1155, 1202
BsaHI	GRCGYC	2 cut(s)	131, 140
BsaJI	CCNNGG	3 cut(s)	1743, 1857, 1870
BsaXI	ACNNNNNCTCC	4 cut(s)	951, 981, 1910, 1940
Bsc4I	CCNNNNNNNGG	5 cut(s)	161, 257, 935, 1078, 1995
Bse118I	RCCGGY	1 cut(s)	1883
Bse1I	ACTGG	7 cut(s)	121, 283, 313, 457, 904, 1072, 1734
Bse3DI	GCAATG	2 cut(s)	87, 99
BseBI	CCWGG	4 cut(s)	1003, 1754, 1859, 1916
BseDI	CCNNGG	3 cut(s)	1743, 1857, 1870
BseGI	GGATG	1 cut(s)	1513
BseLI	CCNNNNNNNGG	5 cut(s)	161, 257, 935, 1078, 1995
BseMI	GCAATG	2 cut(s)	87, 99
BseMII	CTCAG	4 cut(s)	681, 1509, 1626, 1869
BseNI	ACTGG	7 cut(s)	121, 283, 313, 457, 904, 1072, 1734
BseRI	GAGGAG	7 cut(s)	17, 26, 29, 32, 35, 52, 1095
BseSI	GKGCMC	1 cut(s)	770
BseXI	GCAGC	5 cut(s)	319, 490, 1167, 1412, 1726
BseYI	CCCAGC	1 cut(s)	250
Bsh1236I	CGCG	1 cut(s)	149
BshFI	GGCC	7 cut(s)	101, 198, 240, 264, 1071, 1883, 1999
BshNI	GGYRCC	1 cut(s)	1749
BsiHKAI	GWGCWC	3 cut(s)	78, 770, 1037
BsiHKCI	CYCGRG	2 cut(s)	866, 1760
BsiSI	CCGG	1 cut(s)	1884
BslFI	GGGAC	1 cut(s)	571
BslI	CCNNNNNNNGG	5 cut(s)	161, 257, 935, 1078, 1995
BsmAI	GTCTC	5 cut(s)	688, 863, 1107, 1287, 1989
BsmBI	CGTCTC	1 cut(s)	1287
BsmFI	GGGAC	1 cut(s)	571
BsnI	GGCC	7 cut(s)	101, 198, 240, 264, 1071, 1883, 1999
BsoBI	CYCGRG	2 cut(s)	866, 1760
Bsp119I	TTCGAA	1 cut(s)	652
Bsp1286I	GDGCHC	4 cut(s)	78, 770, 990, 1037
Bsp143I	GATC	6 cut(s)	315, 954, 1138, 1284, 1298, 1560
Bsp1720I	GCTNAGC	1 cut(s)	2027
Bsp19I	CCATGG	1 cut(s)	1743
BspACI	CCGC	5 cut(s)	143, 149, 159, 162, 261
BspANI	GGCC	7 cut(s)	101, 198, 240, 264, 1071, 1883, 1999
BspCNI	CTCAG	4 cut(s)	680, 1510, 1627, 1870
BspFNI	CGCG	1 cut(s)	149
BspLI	GGNNCC	8 cut(s)	111, 153, 239, 785, 1140, 1286, 1751, 1758
BspMI	ACCTGC	1 cut(s)	1615
BspPI	GGATC	7 cut(s)	310, 1133, 1146, 1279, 1292, 1306, 1568
BspQI	GCTCTTC	2 cut(s)	995, 1971
BspT104I	TTCGAA	1 cut(s)	652
BspT107I	GGYRCC	1 cut(s)	1749
BsrDI	GCAATG	2 cut(s)	87, 99
BsrFI	RCCGGY	1 cut(s)	1883
BsrI	ACTGG	7 cut(s)	121, 283, 313, 457, 904, 1072, 1734
BssAI	RCCGGY	1 cut(s)	1883
BssECI	CCNNGG	3 cut(s)	1743, 1857, 1870
BssMI	GATC	6 cut(s)	315, 954, 1138, 1284, 1298, 1560
BssNI	GRCGYC	2 cut(s)	131, 140
BssSI	CACGAG	2 cut(s)	393, 1979
BssT1I	CCWWGG	2 cut(s)	1743, 1870
Bst2BI	CACGAG	2 cut(s)	393, 1979
Bst2UI	CCWGG	4 cut(s)	1003, 1754, 1859, 1916
Bst4CI	ACNGT	2 cut(s)	765, 1491
Bst6I	CTCTTC	4 cut(s)	995, 1051, 1088, 1971
BstACI	GRCGYC	2 cut(s)	131, 140
BstAPI	GCANNNNNTGC	2 cut(s)	427, 536
BstBI	TTCGAA	1 cut(s)	652
BstC8I	GCNNGC	7 cut(s)	147, 184, 348, 1058, 1218, 1885, 1976
BstDEI	CTNAG	9 cut(s)	491, 667, 1061, 1289, 1518, 1635, 1878, 2027, 2046
BstDSI	CCRYGG	1 cut(s)	1743
BstF5I	GGATG	1 cut(s)	1513
BstFNI	CGCG	1 cut(s)	149
BstH2I	RGCGCY	1 cut(s)	168
BstHHI	GCGC	2 cut(s)	167, 1254
BstKTI	GATC	6 cut(s)	318, 957, 1141, 1287, 1301, 1563
BstMAI	GTCTC	5 cut(s)	688, 863, 1107, 1287, 1989
BstMBI	GATC	6 cut(s)	315, 954, 1138, 1284, 1298, 1560
BstNI	CCWGG	4 cut(s)	1003, 1754, 1859, 1916
BstNSI	RCATGY	1 cut(s)	796
BstSCI	CCNGG	4 cut(s)	1001, 1752, 1857, 1914
BstSFI	CTRYAG	2 cut(s)	465, 1611
BstSLI	GKGCMC	1 cut(s)	770
BstUI	CGCG	1 cut(s)	149
BstV1I	GCAGC	5 cut(s)	319, 490, 1167, 1412, 1726
BstV2I	GAAGAC	2 cut(s)	827, 1708
BstX2I	RGATCY	3 cut(s)	315, 1138, 1284
BstYI	RGATCY	3 cut(s)	315, 1138, 1284
BsuRI	GGCC	7 cut(s)	101, 198, 240, 264, 1071, 1883, 1999
BtgI	CCRYGG	1 cut(s)	1743
BtsCI	GGATG	1 cut(s)	1513
BtsI	GCAGTG	3 cut(s)	510, 1165, 1519
BtsIMutI	CAGTG	6 cut(s)	276, 510, 911, 1165, 1519, 1741
BveI	ACCTGC	1 cut(s)	1615
Cac8I	GCNNGC	7 cut(s)	147, 184, 348, 1058, 1218, 1885, 1976
CfoI	GCGC	2 cut(s)	167, 1254
Cfr10I	RCCGGY	1 cut(s)	1883
Cfr13I	GGNCC	5 cut(s)	109, 151, 196, 238, 599
CseI	GACGC	2 cut(s)	148, 568
Csp6I	GTAC	6 cut(s)	286, 358, 1065, 1165, 1750, 1765
CviAII	CATG	6 cut(s)	135, 793, 1394, 1586, 1723, 1744
CviQI	GTAC	6 cut(s)	286, 358, 1065, 1165, 1750, 1765
DdeI	CTNAG	9 cut(s)	491, 667, 1061, 1289, 1518, 1635, 1878, 2027, 2046
DpnI	GATC	6 cut(s)	317, 956, 1140, 1286, 1300, 1562
DpnII	GATC	6 cut(s)	315, 954, 1138, 1284, 1298, 1560
EaeI	YGGCCR	1 cut(s)	1069
Eam1104I	CTCTTC	4 cut(s)	995, 1051, 1088, 1971
EarI	CTCTTC	4 cut(s)	995, 1051, 1088, 1971
Ecl136II	GAGCTC	1 cut(s)	76
Eco130I	CCWWGG	2 cut(s)	1743, 1870
Eco147I	AGGCCT	1 cut(s)	101
Eco24I	GRGCYC	2 cut(s)	78, 990
Eco47I	GGWCC	3 cut(s)	109, 151, 599
Eco53kI	GAGCTC	1 cut(s)	76
Eco57I	CTGAAG	1 cut(s)	287
Eco88I	CYCGRG	2 cut(s)	866, 1760
EcoICRI	GAGCTC	1 cut(s)	76
EcoRII	CCWGG	4 cut(s)	1001, 1752, 1857, 1914
EcoT14I	CCWWGG	2 cut(s)	1743, 1870
EcoT22I	ATGCAT	2 cut(s)	6, 1810
EcoT38I	GRGCYC	2 cut(s)	78, 990
ErhI	CCWWGG	2 cut(s)	1743, 1870
Esp3I	CGTCTC	1 cut(s)	1287
FaeI	CATG	6 cut(s)	138, 796, 1397, 1589, 1726, 1747
FalI	AAGNNNNNCTT	2 cut(s)	1164, 1196
FaqI	GGGAC	1 cut(s)	571
FatI	CATG	6 cut(s)	134, 792, 1393, 1585, 1722, 1743
FblI	GTMKAC	1 cut(s)	1012
Fnu4HI	GCNGC	9 cut(s)	143, 160, 163, 262, 333, 479, 1156, 1426, 1740
FokI	GGATG	1 cut(s)	1520
FriOI	GRGCYC	2 cut(s)	78, 990
Fsp4HI	GCNGC	9 cut(s)	143, 160, 163, 262, 333, 479, 1156, 1426, 1740
FspBI	CTAG	3 cut(s)	635, 813, 1700
FspI	TGCGCA	1 cut(s)	1253
GlaI	GCGC	2 cut(s)	166, 1253
GluI	GCNGC	9 cut(s)	143, 160, 163, 262, 333, 479, 1156, 1426, 1740
GsaI	CCCAGC	1 cut(s)	254
GsuI	CTGGAG	3 cut(s)	666, 1139, 1937
HaeII	RGCGCY	1 cut(s)	168
HaeIII	GGCC	7 cut(s)	101, 198, 240, 264, 1071, 1883, 1999
HapII	CCGG	1 cut(s)	1884
HgaI	GACGC	2 cut(s)	148, 568
HhaI	GCGC	2 cut(s)	167, 1254
Hin1I	GRCGYC	2 cut(s)	131, 140
Hin1II	CATG	6 cut(s)	138, 796, 1397, 1589, 1726, 1747
Hin6I	GCGC	2 cut(s)	165, 1252
HinP1I	GCGC	2 cut(s)	165, 1252
HincII	GTYRAC	4 cut(s)	450, 522, 1013, 1536
HindII	GTYRAC	4 cut(s)	450, 522, 1013, 1536
HindIII	AAGCTT	1 cut(s)	1228
HpaI	GTTAAC	1 cut(s)	522
HpaII	CCGG	1 cut(s)	1884
HphI	GGTGA	6 cut(s)	61, 395, 560, 964, 1846, 1873
Hpy166II	GTNNAC	9 cut(s)	370, 450, 522, 768, 1013, 1087, 1536, 1767, 1949
Hpy188I	TCNGA	8 cut(s)	783, 913, 959, 1051, 1623, 1678, 1933, 1939
Hpy8I	GTNNAC	9 cut(s)	370, 450, 522, 768, 1013, 1087, 1536, 1767, 1949
Hpy99I	CGWCG	2 cut(s)	1014, 1349
HpyAV	CCTTC	9 cut(s)	23, 32, 91, 167, 220, 299, 698, 1357, 1633
HpyCH4III	ACNGT	2 cut(s)	765, 1491
HpyCH4IV	ACGT	3 cut(s)	131, 648, 1846
HpyF3I	CTNAG	9 cut(s)	491, 667, 1061, 1289, 1518, 1635, 1878, 2027, 2046
HpySE526I	ACGT	3 cut(s)	131, 648, 1846
Hsp92I	GRCGYC	2 cut(s)	131, 140
Hsp92II	CATG	6 cut(s)	138, 796, 1397, 1589, 1726, 1747
HspAI	GCGC	2 cut(s)	165, 1252
KpnI	GGTACC	1 cut(s)	1753
KroI	GCCGGC	1 cut(s)	1883
KroNI	GCCGGC	1 cut(s)	1885
KspAI	GTTAAC	1 cut(s)	522
Kzo9I	GATC	6 cut(s)	315, 954, 1138, 1284, 1298, 1560
LguI	GCTCTTC	2 cut(s)	995, 1971
LmnI	GCTCC	5 cut(s)	81, 959, 1032, 1213, 1762
Lsp1109I	GCAGC	5 cut(s)	319, 490, 1167, 1412, 1726
LweI	GCATC	3 cut(s)	613, 926, 1897
MaeI	CTAG	3 cut(s)	635, 813, 1700
MaeII	ACGT	3 cut(s)	131, 648, 1846
MaeIII	GTNAC	7 cut(s)	202, 437, 815, 1306, 1485, 1796, 1861
MalI	GATC	6 cut(s)	317, 956, 1140, 1286, 1300, 1562
MboI	GATC	6 cut(s)	315, 954, 1138, 1284, 1298, 1560
MfeI	CAATTG	2 cut(s)	889, 2038
MflI	RGATCY	3 cut(s)	315, 1138, 1284
MhlI	GDGCHC	4 cut(s)	78, 770, 990, 1037
MlsI	TGGCCA	1 cut(s)	1071
MluNI	TGGCCA	1 cut(s)	1071
MlyI	GAGTC	5 cut(s)	836, 1037, 1646, 1858, 1991
MmeI	TCCRAC	1 cut(s)	552
Mox20I	TGGCCA	1 cut(s)	1071
Mph1103I	ATGCAT	2 cut(s)	6, 1810
MroNI	GCCGGC	1 cut(s)	1883
MroXI	GAANNNNTTC	1 cut(s)	1927
MscI	TGGCCA	1 cut(s)	1071
MseI	TTAA	6 cut(s)	483, 521, 585, 699, 1388, 1503
MslI	CAYNNNNRTG	4 cut(s)	92, 297, 1590, 1859
Msp20I	TGGCCA	1 cut(s)	1071
MspA1I	CMGCKG	2 cut(s)	254, 1634
MspI	CCGG	1 cut(s)	1884
MspR9I	CCNGG	4 cut(s)	1003, 1754, 1859, 1916
MunI	CAATTG	2 cut(s)	889, 2038
MvaI	CCWGG	4 cut(s)	1003, 1754, 1859, 1916
MvnI	CGCG	1 cut(s)	149
NaeI	GCCGGC	1 cut(s)	1885
NcoI	CCATGG	1 cut(s)	1743
NdeII	GATC	6 cut(s)	315, 954, 1138, 1284, 1298, 1560
NgoMIV	GCCGGC	1 cut(s)	1883
NlaIII	CATG	6 cut(s)	138, 796, 1397, 1589, 1726, 1747
NlaIV	GGNNCC	8 cut(s)	111, 153, 239, 785, 1140, 1286, 1751, 1758
NmuCI	GTSAC	3 cut(s)	202, 1796, 1861
NsbI	TGCGCA	1 cut(s)	1253
NsiI	ATGCAT	2 cut(s)	6, 1810
NspI	RCATGY	1 cut(s)	796
NspV	TTCGAA	1 cut(s)	652
OliI	CACNNNNGTG	1 cut(s)	1859
PceI	AGGCCT	1 cut(s)	101
PciSI	GCTCTTC	2 cut(s)	995, 1971
PdiI	GCCGGC	1 cut(s)	1885
PdmI	GAANNNNTTC	1 cut(s)	1927
PfeI	GAWTC	5 cut(s)	244, 708, 1399, 1679, 1934
PflMI	CCANNNNNTGG	2 cut(s)	257, 935
PkrI	GCNGC	9 cut(s)	144, 161, 164, 263, 334, 480, 1157, 1427, 1741
PleI	GAGTC	5 cut(s)	836, 1037, 1646, 1858, 1990
PpsI	GAGTC	5 cut(s)	836, 1037, 1646, 1858, 1990
Psp124BI	GAGCTC	1 cut(s)	78
Psp6I	CCWGG	4 cut(s)	1001, 1752, 1857, 1914
PspFI	CCCAGC	1 cut(s)	250
PspGI	CCWGG	4 cut(s)	1001, 1752, 1857, 1914
PspN4I	GGNNCC	8 cut(s)	111, 153, 239, 785, 1140, 1286, 1751, 1758
PspPI	GGNCC	5 cut(s)	109, 151, 196, 238, 599
PsuI	RGATCY	3 cut(s)	315, 1138, 1284
PvuII	CAGCTG	2 cut(s)	254, 1634
RsaI	GTAC	6 cut(s)	287, 359, 1066, 1166, 1751, 1766
RsaNI	GTAC	6 cut(s)	286, 358, 1065, 1165, 1750, 1765
RseI	CAYNNNNRTG	4 cut(s)	92, 297, 1590, 1859
SacI	GAGCTC	1 cut(s)	78
SalI	GTCGAC	1 cut(s)	1011
SapI	GCTCTTC	2 cut(s)	995, 1971
SaqAI	TTAA	6 cut(s)	483, 521, 585, 699, 1388, 1503
SatI	GCNGC	9 cut(s)	143, 160, 163, 262, 333, 479, 1156, 1426, 1740
Sau3AI	GATC	6 cut(s)	315, 954, 1138, 1284, 1298, 1560
Sau96I	GGNCC	5 cut(s)	109, 151, 196, 238, 599
ScaI	AGTACT	1 cut(s)	1066
SchI	GAGTC	5 cut(s)	836, 1037, 1646, 1858, 1991
ScrFI	CCNGG	4 cut(s)	1003, 1754, 1859, 1916
SduI	GDGCHC	4 cut(s)	78, 770, 990, 1037
SfaNI	GCATC	3 cut(s)	613, 926, 1897
SfcI	CTRYAG	2 cut(s)	465, 1611
SfuI	TTCGAA	1 cut(s)	652
SinI	GGWCC	3 cut(s)	109, 151, 599
SmiMI	CAYNNNNRTG	4 cut(s)	92, 297, 1590, 1859
SmlI	CTYRAG	2 cut(s)	1170, 1181
SmoI	CTYRAG	2 cut(s)	1170, 1181
SseBI	AGGCCT	1 cut(s)	101
SsiI	CCGC	5 cut(s)	143, 149, 159, 162, 261
SspI	AATATT	1 cut(s)	880
SspMI	CTAG	3 cut(s)	635, 813, 1700
SstI	GAGCTC	1 cut(s)	78
StuI	AGGCCT	1 cut(s)	101
StyD4I	CCNGG	4 cut(s)	1001, 1752, 1857, 1914
StyI	CCWWGG	2 cut(s)	1743, 1870
TaaI	ACNGT	2 cut(s)	765, 1491
TaiI	ACGT	3 cut(s)	134, 651, 1849
TaqI	TCGA	3 cut(s)	652, 1012, 1497
TatI	WGTACW	2 cut(s)	1064, 1764
TauI	GCSGC	4 cut(s)	145, 162, 165, 264
TfiI	GAWTC	5 cut(s)	244, 708, 1399, 1679, 1934
Tru1I	TTAA	6 cut(s)	483, 521, 585, 699, 1388, 1503
Tru9I	TTAA	6 cut(s)	483, 521, 585, 699, 1388, 1503
TscAI	CASTG	6 cut(s)	283, 510, 911, 1165, 1519, 1741
TseFI	GTSAC	3 cut(s)	202, 1796, 1861
TseI	GCWGC	5 cut(s)	332, 478, 1155, 1425, 1739
Tsp45I	GTSAC	3 cut(s)	202, 1796, 1861
TspDTI	ATGAA	5 cut(s)	110, 717, 872, 1491, 1607
TspGWI	ACGGA	1 cut(s)	1311
TspRI	CASTG	6 cut(s)	283, 510, 911, 1165, 1519, 1741
Van91I	CCANNNNNTGG	2 cut(s)	257, 935
VneI	GTGCAC	1 cut(s)	766
VpaK11BI	GGWCC	3 cut(s)	109, 151, 599
XapI	RAATTY	4 cut(s)	387, 1198, 1262, 1355
XceI	RCATGY	1 cut(s)	796
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1741, 1750
XmiI	GTMKAC	1 cut(s)	1012
XmnI	GAANNNNTTC	1 cut(s)	1927
XspI	CTAG	3 cut(s)	635, 813, 1700
ZraI	GACGTC	1 cut(s)	132
ZrmI	AGTACT	1 cut(s)	1066
Zsp2I	ATGCAT	2 cut(s)	6, 1810

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.