MD07G1129800.v1.1

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0020398
Physical Location & Seq
Reverse (-)
18564028 .. 18564711
684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1129800.v1.1.491

Sequence Viewer

Length: 618 bp
ATGGCACCAAAGTTAGGAATTTCACTAGCATTCCTCCTTTCAGTAACCCTAGCTCTACAATTCGGAGCTGAAGCTGATGGCATTGCCATTTACTGGGGACAAAATGAGAATGAGGGTACCTTGGAGGAGACTTGTGCCACATGCAACTATGAATATGTCATCTTGGCTTTCCTTCCAACTTTTGGCAATGGCCAGACCCCCATGATCAACCTGGCTGGACACTGCGATCCATACATGAATGGCTGCACCGGTTTGAGCTCCGACATTAAATCATGCCAAGCGAAAGGTCTCAAGGTAGCAACGTATTTGTGGAATAACTTTTTGGGGGGAACCTCTTCATTGCGCCCGCTGGGTGACGTTGTTTTGGATGGAATTGATTTTGATATCGAGGGAGGAACACCCATACATTGGGATGACCTAGCAAGGTTCCTCTCTGCTTATAGTAATAAAGAGAAGAAAGTTTACTTCACTGCGGCTCCTCAGTGTCCTTTCCCTGATGCTTGGGTTGGAAGTGCCCTAAAGACAGGCCTTTTCGACAACGTTTGGGTGCAATTCTACAACAACCCACTATGCCAATACTCGCCGTGGGATCATTTCCGGCGACAAAGATCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

206

Amino Acids

22.68

Weight (kDa)

4.82

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 28 - 187 1.1e-06 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020398)

Species Orthologous Gene IDs
malus_domestica MD07G1129800.v1.1
rosa_chinensis RchiOBHm_Chr2g0156261
rosa_multiflora Rmu_sc0002071.1_g000011 Rmu_sc0007072.1_g000003
rosa_samantha Rh1AG436600 Rh4AG100600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 116
AccB1I GGYRCC 2 cut(s) 4, 116
AccB7I CCANNNNNTGG 3 cut(s) 93, 182, 408
AciI CCGC 2 cut(s) 347, 473
AclI AACGTT 1 cut(s) 540
AclWI GGATC 2 cut(s) 221, 597
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 90
AfaI GTAC 1 cut(s) 118
AfiI CCNNNNNNNGG 4 cut(s) 14, 93, 182, 408
AgeI ACCGGT 1 cut(s) 248
AjnI CCWGG 1 cut(s) 210
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
AluBI AGCT 4 cut(s) 53, 68, 74, 258
AluI AGCT 4 cut(s) 53, 68, 74, 258
Alw21I GWGCWC 1 cut(s) 260
Alw26I GTCTC 2 cut(s) 122, 293
AlwI GGATC 2 cut(s) 221, 597
AoxI GGCC 2 cut(s) 190, 526
ApeKI GCWGC 1 cut(s) 243
ApoI RAATTY 1 cut(s) 18
AsiGI ACCGGT 1 cut(s) 248
Asp700I GAANNNNTTC 1 cut(s) 334
Asp718I GGTACC 1 cut(s) 116
AspLEI GCGC 1 cut(s) 345
AsuHPI GGTGA 1 cut(s) 365
BaeGI GKGCMC 1 cut(s) 517
BalI TGGCCA 1 cut(s) 192
BanI GGYRCC 2 cut(s) 4, 116
BanII GRGCYC 1 cut(s) 260
BarI GAAGNNNNNNTAC 2 cut(s) 446, 478
Bbv12I GWGCWC 1 cut(s) 260
BbvI GCAGC 1 cut(s) 230
BccI CCATC 2 cut(s) 71, 362
BceAI ACGGC 1 cut(s) 568
BciT130I CCWGG 1 cut(s) 212
BclI TGATCA 1 cut(s) 204
BcoDI GTCTC 2 cut(s) 122, 293
BfaI CTAG 4 cut(s) 26, 50, 419, 616
BglII AGATCT 1 cut(s) 608
BisI GCNGC 2 cut(s) 244, 474
BlsI GCNGC 2 cut(s) 245, 475
Bme1390I CCNGG 1 cut(s) 212
BmiI GGNNCC 5 cut(s) 6, 118, 331, 428, 477
BmrFI CCNGG 1 cut(s) 212
BmrI ACTGGG 1 cut(s) 103
BmsI GCATC 1 cut(s) 487
BmuI ACTGGG 1 cut(s) 103
BpuEI CTTGAG 1 cut(s) 275
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 2 cut(s) 120, 584
BsaWI WCCGGW 1 cut(s) 248
BsaXI ACNNNNNCTCC 2 cut(s) 460, 490
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 93, 182, 408
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 98
Bse3DI GCAATG 3 cut(s) 81, 193, 338
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 2 cut(s) 120, 584
BseGI GGATG 2 cut(s) 373, 418
BseLI CCNNNNNNNGG 4 cut(s) 14, 93, 182, 408
BseMI GCAATG 3 cut(s) 81, 193, 338
BseMII CTCAG 1 cut(s) 494
BseNI ACTGG 1 cut(s) 98
BseRI GAGGAG 2 cut(s) 140, 468
BseSI GKGCMC 1 cut(s) 517
BseXI GCAGC 1 cut(s) 230
BseYI CCCAGC 1 cut(s) 349
BsgI GTGCAG 1 cut(s) 229
BshFI GGCC 2 cut(s) 192, 528
BshNI GGYRCC 2 cut(s) 4, 116
BshTI ACCGGT 1 cut(s) 248
BsiHKAI GWGCWC 1 cut(s) 260
BsiSI CCGG 2 cut(s) 249, 598
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 4 cut(s) 14, 93, 182, 408
BsmAI GTCTC 2 cut(s) 122, 293
BsmFI GGGAC 1 cut(s) 111
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 2 cut(s) 192, 528
Bso31I GGTCTC 1 cut(s) 293
Bsp1286I GDGCHC 2 cut(s) 260, 517
Bsp143I GATC 4 cut(s) 204, 226, 589, 608
BspACI CCGC 2 cut(s) 347, 473
BspANI GGCC 2 cut(s) 192, 528
BspCNI CTCAG 1 cut(s) 493
BspLI GGNNCC 5 cut(s) 6, 118, 331, 428, 477
BspPI GGATC 2 cut(s) 221, 597
BspT107I GGYRCC 2 cut(s) 4, 116
BspTNI GGTCTC 1 cut(s) 293
BsrDI GCAATG 3 cut(s) 81, 193, 338
BsrFI RCCGGY 1 cut(s) 248
BsrI ACTGG 1 cut(s) 98
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 2 cut(s) 120, 584
BssMI GATC 4 cut(s) 204, 226, 589, 608
BssT1I CCWWGG 1 cut(s) 120
Bst2UI CCWGG 1 cut(s) 212
Bst6I CTCTTC 1 cut(s) 340
BstC8I GCNNGC 1 cut(s) 347
BstDEI CTNAG 1 cut(s) 480
BstDSI CCRYGG 1 cut(s) 584
BstF5I GGATG 2 cut(s) 373, 418
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 4 cut(s) 207, 229, 592, 611
BstMAI GTCTC 2 cut(s) 122, 293
BstMBI GATC 4 cut(s) 204, 226, 589, 608
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 210
BstSLI GKGCMC 1 cut(s) 517
BstV1I GCAGC 1 cut(s) 230
BstX2I RGATCY 1 cut(s) 608
BstYI RGATCY 1 cut(s) 608
BsuRI GGCC 2 cut(s) 192, 528
BtgI CCRYGG 1 cut(s) 584
BtsCI GGATG 2 cut(s) 373, 418
BtsI GCAGTG 2 cut(s) 220, 468
BtsIMutI CAGTG 3 cut(s) 220, 468, 488
Cac8I GCNNGC 1 cut(s) 347
CfoI GCGC 1 cut(s) 345
Cfr10I RCCGGY 1 cut(s) 248
Csp6I GTAC 1 cut(s) 117
CspAI ACCGGT 1 cut(s) 248
CviAII CATG 4 cut(s) 141, 202, 235, 273
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 1 cut(s) 480
DpnI GATC 4 cut(s) 206, 228, 591, 610
DpnII GATC 4 cut(s) 204, 226, 589, 608
EaeI YGGCCR 1 cut(s) 190
Eam1104I CTCTTC 1 cut(s) 340
EarI CTCTTC 1 cut(s) 340
Ecl136II GAGCTC 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 120
Eco147I AGGCCT 1 cut(s) 528
Eco24I GRGCYC 1 cut(s) 260
Eco31I GGTCTC 1 cut(s) 293
Eco32I GATATC 1 cut(s) 385
Eco53kI GAGCTC 1 cut(s) 258
Eco57I CTGAAG 1 cut(s) 90
EcoICRI GAGCTC 1 cut(s) 258
EcoRII CCWGG 1 cut(s) 210
EcoRV GATATC 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 120
EcoT38I GRGCYC 1 cut(s) 260
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 4 cut(s) 144, 205, 238, 276
FaqI GGGAC 1 cut(s) 111
FatI CATG 4 cut(s) 140, 201, 234, 272
FauI CCCGC 1 cut(s) 354
FbaI TGATCA 1 cut(s) 204
Fnu4HI GCNGC 2 cut(s) 244, 474
FokI GGATG 2 cut(s) 380, 425
FriOI GRGCYC 1 cut(s) 260
Fsp4HI GCNGC 2 cut(s) 244, 474
FspBI CTAG 4 cut(s) 26, 50, 419, 616
GlaI GCGC 1 cut(s) 344
GluI GCNGC 2 cut(s) 244, 474
GsaI CCCAGC 1 cut(s) 353
HaeIII GGCC 2 cut(s) 192, 528
HapII CCGG 2 cut(s) 249, 598
HhaI GCGC 1 cut(s) 345
Hin1II CATG 4 cut(s) 144, 205, 238, 276
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HpaII CCGG 2 cut(s) 249, 598
HphI GGTGA 1 cut(s) 365
Hpy166II GTNNAC 1 cut(s) 463
Hpy188I TCNGA 2 cut(s) 65, 262
Hpy8I GTNNAC 1 cut(s) 463
HpyAV CCTTC 1 cut(s) 182
HpyCH4IV ACGT 3 cut(s) 302, 357, 540
HpyCH4V TGCA 3 cut(s) 144, 246, 550
HpyF3I CTNAG 1 cut(s) 480
HpySE526I ACGT 3 cut(s) 302, 357, 540
Hsp92II CATG 4 cut(s) 144, 205, 238, 276
HspAI GCGC 1 cut(s) 343
KpnI GGTACC 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 204
Kzo9I GATC 4 cut(s) 204, 226, 589, 608
LmnI GCTCC 3 cut(s) 65, 263, 481
Lsp1109I GCAGC 1 cut(s) 230
LweI GCATC 1 cut(s) 487
MaeI CTAG 4 cut(s) 26, 50, 419, 616
MaeII ACGT 3 cut(s) 302, 357, 540
MaeIII GTNAC 2 cut(s) 43, 353
MalI GATC 4 cut(s) 206, 228, 591, 610
MboI GATC 4 cut(s) 204, 226, 589, 608
MboII GAAGA 2 cut(s) 327, 466
MflI RGATCY 1 cut(s) 608
MhlI GDGCHC 2 cut(s) 260, 517
MlsI TGGCCA 1 cut(s) 192
MluCI AATT 4 cut(s) 18, 59, 372, 551
MluNI TGGCCA 1 cut(s) 192
MmeI TCCRAC 3 cut(s) 200, 285, 487
MnlI CCTC 8 cut(s) 44, 106, 118, 343, 382, 386, 440, 489
Mox20I TGGCCA 1 cut(s) 192
MroXI GAANNNNTTC 1 cut(s) 334
MscI TGGCCA 1 cut(s) 192
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 1 cut(s) 411
Msp20I TGGCCA 1 cut(s) 192
MspA1I CMGCKG 1 cut(s) 349
MspI CCGG 2 cut(s) 249, 598
MspR9I CCNGG 1 cut(s) 212
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 1 cut(s) 212
NdeII GATC 4 cut(s) 204, 226, 589, 608
NlaIII CATG 4 cut(s) 144, 205, 238, 276
NlaIV GGNNCC 5 cut(s) 6, 118, 331, 428, 477
NmuCI GTSAC 1 cut(s) 353
NspI RCATGY 1 cut(s) 144
PceI AGGCCT 1 cut(s) 528
PctI GAATGC 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 334
PflMI CCANNNNNTGG 3 cut(s) 93, 182, 408
PinAI ACCGGT 1 cut(s) 248
PkrI GCNGC 2 cut(s) 245, 475
Psp124BI GAGCTC 1 cut(s) 260
Psp1406I AACGTT 1 cut(s) 540
Psp6I CCWGG 1 cut(s) 210
PspFI CCCAGC 1 cut(s) 349
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 5 cut(s) 6, 118, 331, 428, 477
PsuI RGATCY 1 cut(s) 608
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
RseI CAYNNNNRTG 1 cut(s) 411
SacI GAGCTC 1 cut(s) 260
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 2 cut(s) 244, 474
Sau3AI GATC 4 cut(s) 204, 226, 589, 608
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 2 cut(s) 260, 517
SfaNI GCATC 1 cut(s) 487
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 4 cut(s) 18, 59, 372, 551
SseBI AGGCCT 1 cut(s) 528
SsiI CCGC 2 cut(s) 347, 473
SspMI CTAG 4 cut(s) 26, 50, 419, 616
SstI GAGCTC 1 cut(s) 260
StuI AGGCCT 1 cut(s) 528
StyD4I CCNGG 1 cut(s) 210
StyI CCWWGG 1 cut(s) 120
TaiI ACGT 3 cut(s) 305, 360, 543
TaqI TCGA 2 cut(s) 387, 534
TasI AATT 4 cut(s) 18, 59, 372, 551
TauI GCSGC 1 cut(s) 476
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 3 cut(s) 227, 475, 488
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 1 cut(s) 243
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 3 cut(s) 165, 251, 327
TspRI CASTG 3 cut(s) 227, 475, 488
Van91I CCANNNNNTGG 3 cut(s) 93, 182, 408
XapI RAATTY 1 cut(s) 18
XceI RCATGY 1 cut(s) 144
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XmnI GAANNNNTTC 1 cut(s) 334
XspI CTAG 4 cut(s) 26, 50, 419, 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.