Rh4AG100600

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0020398
Physical Location & Seq
Reverse (-)
21442743 .. 21443042
300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG100600.1

Sequence Viewer

Length: 300 bp
ATGGCTTCCAAGTCTATATTTTCAGTAACAATATTGCTATTATTTCTGGCTGTTGGATCCAATGCAGGTGGAATTGCTATATATTGGGGCCAGAATGGGAATGAAGGCACATTAGCGGAAACATGTGCTTCAGGGAACTACAAATTCGTAAACATAGCTTTCCTCTCATCATTTGGTAATGGCCAAACCCCTACCATAAACCTCGCCGGTCACTGTGACCCATCCACCAACGAATGCACCAAGTTGAGCCCCGAGATCAAGTCATACCAGGCCAAAGGCATTAAGGTCATACTTCCATAG

Protein Analysis

99

Amino Acids

10.37

Weight (kDa)

7.7

Isoelectric Point (pI)

26.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020398)

Species Orthologous Gene IDs
malus_domestica MD07G1129800.v1.1
rosa_chinensis RchiOBHm_Chr2g0156261
rosa_multiflora Rmu_sc0002071.1_g000011 Rmu_sc0007072.1_g000003
rosa_samantha Rh1AG436600 Rh4AG100600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 56
Acc36I ACCTGC 1 cut(s) 56
AciI CCGC 1 cut(s) 116
AclWI GGATC 2 cut(s) 51, 64
AcoI YGGCCR 1 cut(s) 181
AcsI RAATTY 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 114
AflIII ACRYGT 1 cut(s) 122
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 1 cut(s) 158
AluI AGCT 1 cut(s) 158
AlwI GGATC 2 cut(s) 51, 64
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 3 cut(s) 88, 181, 270
ApoI RAATTY 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 88
AvaI CYCGRG 1 cut(s) 251
BalI TGGCCA 1 cut(s) 183
BamHI GGATCC 1 cut(s) 56
BanII GRGCYC 1 cut(s) 251
BccI CCATC 1 cut(s) 229
BciT130I CCWGG 1 cut(s) 269
BfuAI ACCTGC 1 cut(s) 56
Bme1390I CCNGG 1 cut(s) 269
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 88
BmiI GGNNCC 2 cut(s) 58, 89
BmrFI CCNGG 1 cut(s) 269
Bse118I RCCGGY 1 cut(s) 206
BseBI CCWGG 1 cut(s) 269
BseGI GGATG 1 cut(s) 221
BshFI GGCC 3 cut(s) 90, 183, 272
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 1 cut(s) 207
BsmI GAATGC 1 cut(s) 239
BsnI GGCC 3 cut(s) 90, 183, 272
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 251
Bsp143I GATC 2 cut(s) 56, 255
BspACI CCGC 1 cut(s) 116
BspANI GGCC 3 cut(s) 90, 183, 272
BspLI GGNNCC 2 cut(s) 58, 89
BspMI ACCTGC 1 cut(s) 56
BspPI GGATC 2 cut(s) 51, 64
BsrFI RCCGGY 1 cut(s) 206
BssAI RCCGGY 1 cut(s) 206
BssMI GATC 2 cut(s) 56, 255
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 215
BstF5I GGATG 1 cut(s) 221
BstKTI GATC 2 cut(s) 59, 258
BstMBI GATC 2 cut(s) 56, 255
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 267
BstX2I RGATCY 1 cut(s) 56
BstYI RGATCY 1 cut(s) 56
BsuRI GGCC 3 cut(s) 90, 183, 272
BtsCI GGATG 1 cut(s) 221
BtsIMutI CAGTG 1 cut(s) 211
BveI ACCTGC 1 cut(s) 56
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 1 cut(s) 88
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 1 cut(s) 123
CviJI RGCY 7 cut(s) 5, 50, 90, 158, 183, 249, 272
CviKI_1 RGCY 7 cut(s) 5, 50, 90, 158, 183, 249, 272
DpnI GATC 2 cut(s) 58, 257
DpnII GATC 2 cut(s) 56, 255
EaeI YGGCCR 1 cut(s) 181
Eco24I GRGCYC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 251
FaeI CATG 1 cut(s) 126
FaiI YATR 9 cut(s) 17, 80, 82, 124, 155, 197, 265, 290, 298
FatI CATG 1 cut(s) 122
FokI GGATG 1 cut(s) 208
FriOI GRGCYC 1 cut(s) 251
HaeIII GGCC 3 cut(s) 90, 183, 272
HapII CCGG 1 cut(s) 207
Hin1II CATG 1 cut(s) 126
HpaII CCGG 1 cut(s) 207
Hpy166II GTNNAC 1 cut(s) 151
Hpy8I GTNNAC 1 cut(s) 151
HpyAV CCTTC 1 cut(s) 98
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4V TGCA 2 cut(s) 65, 237
Hsp92II CATG 1 cut(s) 126
Kzo9I GATC 2 cut(s) 56, 255
LpnPI CCDG 7 cut(s) 32, 51, 104, 117, 220, 254, 281
MaeIII GTNAC 3 cut(s) 25, 209, 215
MalI GATC 2 cut(s) 58, 257
MboI GATC 2 cut(s) 56, 255
MflI RGATCY 1 cut(s) 56
MhlI GDGCHC 1 cut(s) 251
MlsI TGGCCA 1 cut(s) 183
MluCI AATT 2 cut(s) 72, 143
MluNI TGGCCA 1 cut(s) 183
MmeI TCCRAC 1 cut(s) 34
MnlI CCTC 2 cut(s) 173, 212
Mox20I TGGCCA 1 cut(s) 183
MscI TGGCCA 1 cut(s) 183
MseI TTAA 1 cut(s) 282
Msp20I TGGCCA 1 cut(s) 183
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 269
Mva1269I GAATGC 1 cut(s) 239
MvaI CCWGG 1 cut(s) 269
NdeII GATC 2 cut(s) 56, 255
NlaIII CATG 1 cut(s) 126
NlaIV GGNNCC 2 cut(s) 58, 89
NmuCI GTSAC 2 cut(s) 209, 215
NspI RCATGY 1 cut(s) 126
PaqCI CACCTGC 1 cut(s) 56
PciI ACATGT 1 cut(s) 122
PctI GAATGC 1 cut(s) 239
PscI ACATGT 1 cut(s) 122
Psp6I CCWGG 1 cut(s) 267
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 2 cut(s) 58, 89
PspPI GGNCC 1 cut(s) 88
PsuI RGATCY 1 cut(s) 56
SaqAI TTAA 1 cut(s) 282
Sau3AI GATC 2 cut(s) 56, 255
Sau96I GGNCC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 269
SduI GDGCHC 1 cut(s) 251
SetI ASST 4 cut(s) 70, 160, 204, 288
Sse9I AATT 2 cut(s) 72, 143
SsiI CCGC 1 cut(s) 116
SspI AATATT 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 215
TasI AATT 2 cut(s) 72, 143
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 218
TseFI GTSAC 2 cut(s) 209, 215
Tsp45I GTSAC 2 cut(s) 209, 215
TspDTI ATGAA 1 cut(s) 117
TspRI CASTG 1 cut(s) 218
XapI RAATTY 1 cut(s) 143
XceI RCATGY 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.