Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0006177

Physical Location & Seq

Genomic Coordinates Reverse (-)

22387773 .. 22392926

Gene Structure 5154 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1153800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3258 bp

ATGGAAACCAAATTCTCGCGATCCAAACAGAAGGCTGACGCGGCAGCTGTGCTCCGGAAATCATGGTACCACCTCAGGCTGTCGGTAAGGCACCCGACCCGGGTTCCCACTTGGGACGCCGTCGTATTGACGGCGGCTAGCCCCGAGCAGGCCGAGCTCTACGAGTGGCAGCTCACTCGCGCCAAGCGGGCGGGCCGCATAGCCGCCTCCACGATCACACTCGCTGTGCCTGACCCCCATGGCCAGCGCATCGGCTCCGGCGCCGCCACTCTCCACGCCATTCACGCCCTCGCCAAGCATTACCGTGCGTTGGGCCTACACTCAGAGGTGGCAGCTACAAGCAATGGTGGTTTTGGGTTCTCGGAGTCAAATCGAAATGCAACTGGCAATGAAATGGATGATGATCTCTCGGAAATGGTTAGCTTCATTGCCAAGAGGCATATATTATTGCTTCATGCTGGTGGTGATTCGAAAAGGGTCCCCTGGGCAAATCCCACGGGGAAAGTTTTCCTGCCACTTCCATATATGGCAGCTGACGACCCTGATGGGCCAGTCCCGCTGCTTTTTGATCATATACTTGCAATTGCTTCTTGTGCAAGACAAGCTTTTAAAAATGAAGGTGGAATATTTACTATGACTGGAGACGTACTTCCATGTTTTGATGCCTCCAACATGGTGCTTCCAGAGGACACATCCTGTATCATAACTGTTCCCATCACCCTTGATATCGCGTCTAACCATGGTGTTGTTGTGGCATCTAAGAGAAGAAATGTAGAAAAAAGTTATCCTGTCAGCTTTGTTGATAATCTCTTACAGAAACCTAGCGTAGACGAACTTGTTAAGAACAATGCAATTCTTGATGATGGTAGAACACTTCTTGACACTGGAATAATAGCGGTTAGAGGTAAAGGGTGGGAGGAGCTTGTTATACTTGCATGTTCCTGCCAACCAATGATTTCGGAGCTTCTGAAGACCAGAAAGGAGATGAGCTTATATGAAGATCTTGTAGCAGCTTGGGTACCTGCAAAGCATGACTGGTTGCGACTGCGCCCTTCAGGCAAAGAACTGGTCAGCAGATTAGGAAAACAGAAGATGTTTAGCTACTGCGCTTATGATTTGTCGTTCTTACATTTTGGAACTTCAAGTGAGATTTTGGATCATCTGAGTGGAGCTGCCTCGGGACTGGTCGGTCGAAGACACCAGTGTTCTATCCCAGCAAGCACTCTATCTGACATTGCAGCATCCGCTGTTCTTCTTTCTAGTAAAATTGCACCTGCTGTCTCGATTGGGGAAGATTCTCTTATATATGATTCAACCATTCCGAGTGGAATGCAAGTTGGTTCTCTGTCCATAGTTGTTGGTATCAATGTTCCAGAAGTAAATAGTAGTGCTGCAGAAAATTCATTTAGATTCATACTTCCGGATCGCCATTGTCTTTGGGAGGTTCCTCTAGTCGGACACACTGGAAGAGTCATAGTATATTGTGGCCTTCATGATAACCCCAAGGTTTCACTCTCTAAGGATGGCACTTTTTGTGGGAAACCCTGGAGGAAGGTTGTGCAAGATTTAGGAATCCAAGAAAATGATCTATGGAGCTCAATGGGTACTCATGAAAAGTGTTTGTGGAATTCAAAAATATTCCCCATTCTTTCTTACTTTGAGATGCTAACTTTGGCATCGTGGTTGATGGGCTTGAGTGATGAAAATTCTGAGCATTTGCTTTCTCTGTGGAGAAGTTCACCTCGTGTTAGTTTAGAGGAGTTGCATAGATCAATTGATTTTTCAAAGATGTGTCATGGTTCAATTGATCATCAAGCAGACCTTGCAGCTGGAATCGCTAAAGCATGCATTAATTATGGGATGTTGGGCCGCAATTTATATCAGTTGTGTGAAGAAGTTCTGCAAAAGGAAGATTTAGGAGTCAAAGTATGTGAGGAATTTCTAAGTCTTTGTCCCGGACTCCTGGAGCAGAACTCTAAGATCATTCCCAAAAGCCGAGCATTCCAGGTTCAAGTTGATCTTCTTCGAGCATGCAGCAATGAAACAACAGCACGTAAGTTGGAGCACAAAGTTTGGAATGCAGTTGCTGATGAAACAGCTTCAGCTGTAAAATATGGTTTTAAAGAACATCTCTATGAGACTCCCAGCGATATCTCTATTCTGTCCCATAAGAACAATGATTTCGACGGCTGTGTGGATCATTCATTCCACCCCAGAAAGGTGAAGGTTGAATTACCAGTTCGTGTGGATTTTGTTGGGGGTTGGAGTGACACTCCTCCATGGAGCTTGGAGCGCTCTGGTTGTGTTCTGAATATGGCAATAAGTTTGGAAGGTTCTCTTCCAATTGGCACCATCATCGAGACAACGAAAAAAACTGGTGTCTGTATTAGTGATGATGGTGGAAATGAGTTACACATTAAGGACCTAACCTCAATTGCCACACCATTTGATGACAACGATCCATTTCGGCTTGTCAAATCCGCACTGCTTGTGACTGGCATTATTCACGAAAATGCTCTTGCATCCAGGGGTCTGCAGATCAGGACATGGGCTTGCGTACCTCGTGGAAGTGGCTTGGGGACTTCTAGCATCTTAGCAGCTGCAGTTGTGAAAGGACTTCTTCAGATTACCGATGGAGATGAAAGTAATGAAAATGTTGCGAGACTTGTTCTTGTATTAGAACAGCTCATGGGGACAGGTGGTGGTTGGCAGGATCAGATTGGGGGTCTCTACCCGGGTATTAAATGTACTTCAAGTTTTCCTGGTATCCCATTGCGACTACAAGTCGTCCCACTCTTGGCTTCCCCACCGTTAATTTCAGAGTTGCAGCAACGCTTGCTTGTGGTTTTTACCGGTCAAGTTCGACTTGCACACCAAGTCTTGCAAAAGGTGGTAACCCGCTACCTCCGAAGGGACAACCTTCTTATATCGAGCATCAAGCGCCTCGCTGAACTGGCCAAGATTGGGAGAGAAGCGTTGATGAACTGTGACATAGACGACCTTGGGGAGATAATGCTAGAGGCTTGGAGGCTGCATCAAGAACTCGACCCTTACTGCAGTAACGAGTTTGTGGATCAACTCTTCAGATTCGCACACCAGTACTGCTCCGGCTACAAGCTCGTGGGTGCCGGAGGCGGAGGCTTTGCCTTGTTGCTTGCCAAGGATGCCAAGCAGGCAAAGGAACTAAGGCTCTTGCTGGAACAAGATTCAAATTTTGACGTCAAAGTATACAACTGGAACGTCTTCTTAGATAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006139 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009117 GO:0009165 GO:0009225 GO:0009226 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016779 GO:0018130 GO:0019438 GO:0019637 GO:0034641 GO:0034654 GO:0042350 GO:0042352 GO:0043094 GO:0043173 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0046368 GO:0046483 GO:0047341 GO:0050201 GO:0055086 GO:0070568 GO:0071704 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00051 ko00520 ko01100 map00051 map00520 map01100

Pfam Domains

Protein Families

Fucokinase GHMP_kinases_C GHMP_kinases_N

Protein Analysis

1086

Amino Acids

119.18

Weight (kDa)

6.19

Isoelectric Point (pI)

38.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fucose_pyrophosphorylase	PF07959	143 - 551	2.6e-112	Fucose pyrophosphorylase domain
GHMP_kinases_N	PF00288	837 - 910	2.4e-10	GHMP kinases N terminal domain
GHMP_kinases_C	PF08544	991 - 1063	7.4e-07	GHMP kinases C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006177)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01220 AT1G01220 AT1G01220 AT1G01220 AT1G01220 AT1G01220 AT1G01220 AT1G01220
fragaria_vesca	FvH4_7g17030 FvH4_7g17030 FvH4_7g17030
malus_domestica	MD07G1153800.v1.1
prunus_persica	Prupe.2G192700_v2.0.a1 Prupe.2G192700_v2.0.a1 Prupe.2G192700_v2.0.a1
pyrus_communis	pycom07g15160
rosa_chinensis	RchiOBHm_Chr1g0360431
rosa_laevigata	RLG00000027808
rosa_multiflora	Rmu_ssc0000368.1_g000084
rosa_roxburghii	Rroxscaffold_4G00296110
rosa_rugosa	Rorug01G0277000 Rorug01G0277100
rosa_samantha	Rh1AG290900 Rh1BG256000 Rh1CG273700 Rh1DG285800
rosa_wichuraiana	Rw1G025760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1282
AasI	GACNNNNNNGTC	1 cut(s)	1188
AatII	GACGTC	1 cut(s)	3222
Acc36I	ACCTGC	2 cut(s)	1030, 1282
Acc65I	GGTACC	2 cut(s)	66, 1018
AccB1I	GGYRCC	6 cut(s)	66, 90, 260, 1018, 2348, 3125
AccI	GTMKAC	2 cut(s)	828, 3228
AccII	CGCG	4 cut(s)	19, 41, 180, 731
AccIII	TCCGGA	2 cut(s)	54, 1420
AclWI	GGATC	7 cut(s)	15, 1164, 1431, 2205, 2453, 2721, 3081
AcoI	YGGCCR	2 cut(s)	241, 2955
AcsI	RAATTY	6 cut(s)	11, 1399, 1627, 1705, 1937, 3211
AcuI	CTGAAG	5 cut(s)	989, 1038, 2085, 2606, 3067
AcyI	GRCGYC	3 cut(s)	117, 261, 3219
AdeI	CACNNNGTG	1 cut(s)	1745
AfaI	GTAC	7 cut(s)	68, 648, 1020, 1606, 2559, 2749, 3101
AfeI	AGCGCT	1 cut(s)	2294
AfiI	CCNNNNNNNGG	8 cut(s)	100, 148, 310, 1454, 2218, 2797, 2911, 2964
AgeI	ACCGGT	1 cut(s)	2852
AgsI	TTSAA	9 cut(s)	1143, 1314, 1632, 1785, 1803, 2012, 2231, 2754, 3210
AhdI	GACNNNNNGTC	1 cut(s)	2783
AjnI	CCWGG	6 cut(s)	482, 1544, 1962, 2004, 2525, 2761
Alw21I	GWGCWC	4 cut(s)	54, 159, 1598, 2067
Alw26I	GTCTC	6 cut(s)	636, 1285, 2132, 2354, 2656, 2732
AlwI	GGATC	7 cut(s)	15, 1164, 1431, 2205, 2453, 2721, 3081
AlwNI	CAGNNNCTG	1 cut(s)	2087
Ama87I	CYCGRG	4 cut(s)	99, 143, 1177, 2734
Aor13HI	TCCGGA	2 cut(s)	54, 1420
Aor51HI	AGCGCT	1 cut(s)	2294
AoxI	GGCC	8 cut(s)	150, 193, 241, 313, 548, 1486, 1867, 2955
ApoI	RAATTY	6 cut(s)	11, 1399, 1627, 1705, 1937, 3211
ArsI	GACNNNNNNTTYG	2 cut(s)	1053, 1085
AseI	ATTAAT	1 cut(s)	1851
AsiGI	ACCGGT	1 cut(s)	2852
Asp700I	GAANNNNTTC	3 cut(s)	506, 1896, 3242
Asp718I	GGTACC	2 cut(s)	66, 1018
AspLEI	GCGC	7 cut(s)	182, 249, 263, 1050, 1109, 2295, 2943
AspS9I	GGNCC	6 cut(s)	193, 313, 478, 548, 1867, 2422
AsuC2I	CCSGG	5 cut(s)	100, 101, 1956, 2735, 2736
AsuHPI	GGTGA	4 cut(s)	476, 709, 1731, 2233
AsuII	TTCGAA	1 cut(s)	470
AsuNHI	GCTAGC	1 cut(s)	137
AvaI	CYCGRG	4 cut(s)	99, 143, 1177, 2734
AvaII	GGWCC	2 cut(s)	478, 2422
AxyI	CCTNAGG	1 cut(s)	74
BalI	TGGCCA	2 cut(s)	243, 2957
BanI	GGYRCC	6 cut(s)	66, 90, 260, 1018, 2348, 3125
BanII	GRGCYC	2 cut(s)	159, 1598
BauI	CACGAG	3 cut(s)	1743, 2562, 3119
BbsI	GAAGAC	3 cut(s)	977, 1201, 3235
Bbv12I	GWGCWC	4 cut(s)	54, 159, 1598, 2067
BccI	CCATC	8 cut(s)	539, 722, 857, 1517, 1681, 2360, 2390, 2627
BceAI	ACGGC	3 cut(s)	104, 147, 2203
BcgI	CGANNNNNNTGC	6 cut(s)	158, 192, 1312, 1346, 2338, 2372
BciT130I	CCWGG	6 cut(s)	484, 1546, 1964, 2006, 2527, 2763
BciVI	GTATCC	1 cut(s)	2777
BclI	TGATCA	2 cut(s)	568, 1807
BcnI	CCSGG	5 cut(s)	100, 101, 1956, 2735, 2736
BcoDI	GTCTC	6 cut(s)	636, 1285, 2132, 2354, 2656, 2732
BfaI	CTAG	6 cut(s)	138, 822, 1260, 1451, 2586, 3017
BfmI	CTRYAG	4 cut(s)	1392, 2534, 2601, 3055
BfoI	RGCGCY	3 cut(s)	264, 2296, 2944
BfuAI	ACCTGC	2 cut(s)	1030, 1282
BfuI	GTATCC	1 cut(s)	2777
BglI	GCCNNNNNGGC	3 cut(s)	188, 1056, 3173
BglII	AGATCT	1 cut(s)	1000
BmcAI	AGTACT	1 cut(s)	3101
Bme18I	GGWCC	2 cut(s)	478, 2422
BmeRI	GACNNNNNGTC	1 cut(s)	2783
BmeT110I	CYCGRG	4 cut(s)	99, 143, 1177, 2734
BmgT120I	GGNCC	6 cut(s)	193, 313, 478, 548, 1867, 2422
BmtI	GCTAGC	1 cut(s)	141
BpiI	GAAGAC	3 cut(s)	977, 1201, 3235
BplI	GAGNNNNNCTC	4 cut(s)	1958, 1990, 2257, 2289
BpmI	CTGGAG	3 cut(s)	660, 1567, 1985
Bpu14I	TTCGAA	1 cut(s)	470
BpuEI	CTTGAG	1 cut(s)	1714
BpuMI	CCSGG	5 cut(s)	100, 101, 1956, 2735, 2736
BsaAI	YACGTR	1 cut(s)	2054
BsaBI	GATNNNNATC	1 cut(s)	402
BsaHI	GRCGYC	3 cut(s)	117, 261, 3219
BsaI	GGTCTC	1 cut(s)	2732
BsaWI	WCCGGW	3 cut(s)	54, 1420, 2852
BsaXI	ACNNNNNCTCC	2 cut(s)	1911, 1941
Bsc4I	CCNNNNNNNGG	8 cut(s)	100, 148, 310, 1454, 2218, 2797, 2911, 2964
Bse118I	RCCGGY	1 cut(s)	2852
Bse21I	CCTNAGG	1 cut(s)	74
Bse3DI	GCAATG	6 cut(s)	349, 394, 426, 1233, 2044, 2771
Bse8I	GATNNNNATC	1 cut(s)	402
BseAI	TCCGGA	2 cut(s)	54, 1420
BseBI	CCWGG	6 cut(s)	484, 1546, 1964, 2006, 2527, 2763
BseGI	GGATG	7 cut(s)	403, 692, 1241, 1528, 1866, 2522, 3169
BseJI	GATNNNNATC	1 cut(s)	402
BseLI	CCNNNNNNNGG	8 cut(s)	100, 148, 310, 1454, 2218, 2797, 2911, 2964
BseMI	GCAATG	6 cut(s)	349, 394, 426, 1233, 2044, 2771
BseMII	CTCAG	4 cut(s)	88, 336, 1154, 1701
BseRI	GAGGAG	3 cut(s)	932, 1772, 2265
BseYI	CCCAGC	2 cut(s)	1213, 2144
Bsh1236I	CGCG	4 cut(s)	19, 41, 180, 731
Bsh1285I	CGRYCG	1 cut(s)	1192
BshFI	GGCC	8 cut(s)	152, 195, 243, 315, 550, 1488, 1869, 2957
BshNI	GGYRCC	6 cut(s)	66, 90, 260, 1018, 2348, 3125
BshTI	ACCGGT	1 cut(s)	2852
BsiEI	CGRYCG	1 cut(s)	1192
BsiHKAI	GWGCWC	4 cut(s)	54, 159, 1598, 2067
BsiHKCI	CYCGRG	4 cut(s)	99, 143, 1177, 2734
BsiSI	CCGG	9 cut(s)	55, 100, 258, 1421, 1956, 2735, 2853, 3108, 3129
BslI	CCNNNNNNNGG	8 cut(s)	100, 148, 310, 1454, 2218, 2797, 2911, 2964
BsmAI	GTCTC	6 cut(s)	636, 1285, 2132, 2354, 2656, 2732
BsmBI	CGTCTC	1 cut(s)	636
BsmI	GAATGC	3 cut(s)	1335, 2000, 2083
BsnI	GGCC	8 cut(s)	152, 195, 243, 315, 550, 1488, 1869, 2957
Bso31I	GGTCTC	1 cut(s)	2732
BsoBI	CYCGRG	4 cut(s)	99, 143, 1177, 2734
Bsp119I	TTCGAA	1 cut(s)	470
Bsp1286I	GDGCHC	4 cut(s)	54, 159, 1598, 2067
Bsp13I	TCCGGA	2 cut(s)	54, 1420
Bsp19I	CCATGG	3 cut(s)	238, 739, 2279
Bsp68I	TCGCGA	1 cut(s)	19
BspANI	GGCC	8 cut(s)	152, 195, 243, 315, 550, 1488, 1869, 2957
BspCNI	CTCAG	4 cut(s)	87, 335, 1155, 1702
BspEI	TCCGGA	2 cut(s)	54, 1420
BspFNI	CGCG	4 cut(s)	19, 41, 180, 731
BspHI	TCATGA	2 cut(s)	1492, 1609
BspMAI	CTGCAG	4 cut(s)	1396, 2538, 2605, 3059
BspMI	ACCTGC	2 cut(s)	1030, 1282
BspOI	GCTAGC	1 cut(s)	141
BspPI	GGATC	7 cut(s)	15, 1164, 1431, 2205, 2453, 2721, 3081
BspT104I	TTCGAA	1 cut(s)	470
BspT107I	GGYRCC	6 cut(s)	66, 90, 260, 1018, 2348, 3125
BspTNI	GGTCTC	1 cut(s)	2732
BsrDI	GCAATG	6 cut(s)	349, 394, 426, 1233, 2044, 2771
BsrFI	RCCGGY	1 cut(s)	2852
BssAI	RCCGGY	1 cut(s)	2852
BssNAI	GTATAC	1 cut(s)	3229
BssNI	GRCGYC	3 cut(s)	117, 261, 3219
BssSI	CACGAG	3 cut(s)	1743, 2562, 3119
BssT1I	CCWWGG	6 cut(s)	238, 739, 1503, 2279, 3001, 3159
Bst1107I	GTATAC	1 cut(s)	3229
Bst2BI	CACGAG	3 cut(s)	1743, 2562, 3119
Bst2UI	CCWGG	6 cut(s)	484, 1546, 1964, 2006, 2527, 2763
Bst4CI	ACNGT	4 cut(s)	305, 709, 2811, 2987
Bst6I	CTCTTC	3 cut(s)	1462, 2343, 3086
BstACI	GRCGYC	3 cut(s)	117, 261, 3219
BstAPI	GCANNNNNTGC	2 cut(s)	1823, 2836
BstBAI	YACGTR	1 cut(s)	2054
BstBI	TTCGAA	1 cut(s)	470
BstDSI	CCRYGG	4 cut(s)	238, 495, 739, 2279
BstEII	GGTNACC	1 cut(s)	2893
BstF5I	GGATG	7 cut(s)	403, 692, 1241, 1528, 1866, 2522, 3169
BstFNI	CGCG	4 cut(s)	19, 41, 180, 731
BstH2I	RGCGCY	3 cut(s)	264, 2296, 2944
BstHHI	GCGC	7 cut(s)	182, 249, 263, 1050, 1109, 2295, 2943
BstMAI	GTCTC	6 cut(s)	636, 1285, 2132, 2354, 2656, 2732
BstMCI	CGRYCG	1 cut(s)	1192
BstNI	CCWGG	6 cut(s)	484, 1546, 1964, 2006, 2527, 2763
BstNSI	RCATGY	3 cut(s)	939, 1848, 2034
BstPI	GGTNACC	1 cut(s)	2893
BstSFI	CTRYAG	4 cut(s)	1392, 2534, 2601, 3055
BstUI	CGCG	4 cut(s)	19, 41, 180, 731
BstV2I	GAAGAC	3 cut(s)	977, 1201, 3235
BstX2I	RGATCY	1 cut(s)	1000
BstYI	RGATCY	1 cut(s)	1000
BstZ17I	GTATAC	1 cut(s)	3229
Bsu36I	CCTNAGG	1 cut(s)	74
BsuI	GTATCC	1 cut(s)	2777
BsuRI	GGCC	8 cut(s)	152, 195, 243, 315, 550, 1488, 1869, 2957
BtgI	CCRYGG	4 cut(s)	238, 495, 739, 2279
BtsCI	GGATG	7 cut(s)	403, 692, 1241, 1528, 1866, 2522, 3169
BtsI	GCAGTG	1 cut(s)	2483
BtsIMutI	CAGTG	4 cut(s)	882, 1208, 1461, 2483
BtuMI	TCGCGA	1 cut(s)	19
BveI	ACCTGC	2 cut(s)	1030, 1282
CaiI	CAGNNNCTG	1 cut(s)	2087
CciI	TCATGA	2 cut(s)	1492, 1609
CfoI	GCGC	7 cut(s)	182, 249, 263, 1050, 1109, 2295, 2943
Cfr10I	RCCGGY	1 cut(s)	2852
Cfr13I	GGNCC	6 cut(s)	193, 313, 478, 548, 1867, 2422
Cfr9I	CCCGGG	2 cut(s)	99, 2734
CseI	GACGC	3 cut(s)	47, 125, 720
Csp6I	GTAC	7 cut(s)	67, 647, 1019, 1605, 2558, 2748, 3100
CspAI	ACCGGT	1 cut(s)	2852
CspCI	CAANNNNNGTGG	2 cut(s)	2428, 2463
CviQI	GTAC	7 cut(s)	67, 647, 1019, 1605, 2558, 2748, 3100
DinI	GGCGCC	1 cut(s)	262
DraI	TTTAAA	2 cut(s)	610, 2122
DraIII	CACNNNGTG	1 cut(s)	1745
DrdI	GACNNNNNNGTC	1 cut(s)	1188
DriI	GACNNNNNGTC	1 cut(s)	2783
DseDI	GACNNNNNNGTC	1 cut(s)	1188
EaeI	YGGCCR	2 cut(s)	241, 2955
Eam1104I	CTCTTC	3 cut(s)	1462, 2343, 3086
Eam1105I	GACNNNNNGTC	1 cut(s)	2783
EarI	CTCTTC	3 cut(s)	1462, 2343, 3086
EciI	GGCGGA	1 cut(s)	3150
Ecl136II	GAGCTC	2 cut(s)	157, 1596
Eco130I	CCWWGG	6 cut(s)	238, 739, 1503, 2279, 3001, 3159
Eco24I	GRGCYC	2 cut(s)	159, 1598
Eco31I	GGTCTC	1 cut(s)	2732
Eco32I	GATATC	2 cut(s)	727, 2152
Eco47I	GGWCC	2 cut(s)	478, 2422
Eco47III	AGCGCT	1 cut(s)	2294
Eco53kI	GAGCTC	2 cut(s)	157, 1596
Eco57I	CTGAAG	5 cut(s)	989, 1038, 2085, 2606, 3067
Eco81I	CCTNAGG	1 cut(s)	74
Eco88I	CYCGRG	4 cut(s)	99, 143, 1177, 2734
Eco91I	GGTNACC	1 cut(s)	2893
EcoICRI	GAGCTC	2 cut(s)	157, 1596
EcoO109I	RGGNCCY	2 cut(s)	478, 2422
EcoO65I	GGTNACC	1 cut(s)	2893
EcoRI	GAATTC	1 cut(s)	1627
EcoRII	CCWGG	6 cut(s)	482, 1544, 1962, 2004, 2525, 2761
EcoRV	GATATC	2 cut(s)	727, 2152
EcoT14I	CCWWGG	6 cut(s)	238, 739, 1503, 2279, 3001, 3159
EcoT22I	ATGCAT	1 cut(s)	1850
EcoT38I	GRGCYC	2 cut(s)	159, 1598
EgeI	GGCGCC	1 cut(s)	262
EheI	GGCGCC	1 cut(s)	262
ErhI	CCWWGG	6 cut(s)	238, 739, 1503, 2279, 3001, 3159
Esp3I	CGTCTC	1 cut(s)	636
FauI	CCCGC	4 cut(s)	180, 184, 564, 2906
FbaI	TGATCA	2 cut(s)	568, 1807
FblI	GTMKAC	2 cut(s)	828, 3228
FokI	GGATG	7 cut(s)	410, 679, 1228, 1535, 1873, 2509, 3176
FriOI	GRGCYC	2 cut(s)	159, 1598
FspBI	CTAG	6 cut(s)	138, 822, 1260, 1451, 2586, 3017
GlaI	GCGC	7 cut(s)	181, 248, 262, 1049, 1108, 2294, 2942
GsaI	CCCAGC	2 cut(s)	1217, 2148
GsuI	CTGGAG	3 cut(s)	660, 1567, 1985
HaeII	RGCGCY	3 cut(s)	264, 2296, 2944
HaeIII	GGCC	8 cut(s)	152, 195, 243, 315, 550, 1488, 1869, 2957
HapII	CCGG	9 cut(s)	55, 100, 258, 1421, 1956, 2735, 2853, 3108, 3129
HgaI	GACGC	3 cut(s)	47, 125, 720
HhaI	GCGC	7 cut(s)	182, 249, 263, 1050, 1109, 2295, 2943
Hin1I	GRCGYC	3 cut(s)	117, 261, 3219
Hin6I	GCGC	7 cut(s)	180, 247, 261, 1048, 1107, 2293, 2941
HinP1I	GCGC	7 cut(s)	180, 247, 261, 1048, 1107, 2293, 2941
HindIII	AAGCTT	1 cut(s)	603
HpaII	CCGG	9 cut(s)	55, 100, 258, 1421, 1956, 2735, 2853, 3108, 3129
HphI	GGTGA	4 cut(s)	476, 709, 1731, 2233
Hpy166II	GTNNAC	3 cut(s)	829, 1739, 3229
Hpy8I	GTNNAC	3 cut(s)	829, 1739, 3229
Hpy99I	CGWCG	2 cut(s)	125, 2189
HpyAV	CCTTC	9 cut(s)	25, 611, 1062, 1499, 1546, 2218, 2324, 2904, 2930
HpyCH4III	ACNGT	4 cut(s)	305, 709, 2811, 2987
HpyCH4IV	ACGT	4 cut(s)	645, 2053, 3219, 3240
HpySE526I	ACGT	4 cut(s)	645, 2053, 3219, 3240
Hsp92I	GRCGYC	3 cut(s)	117, 261, 3219
HspAI	GCGC	7 cut(s)	180, 247, 261, 1048, 1107, 2293, 2941
KasI	GGCGCC	1 cut(s)	260
KflI	GGGWCCC	1 cut(s)	478
Kpn2I	TCCGGA	2 cut(s)	54, 1420
KpnI	GGTACC	2 cut(s)	70, 1022
Ksp22I	TGATCA	2 cut(s)	568, 1807
MaeI	CTAG	6 cut(s)	138, 822, 1260, 1451, 2586, 3017
MaeII	ACGT	4 cut(s)	645, 2053, 3219, 3240
MaeIII	GTNAC	6 cut(s)	2267, 2409, 2491, 2893, 2987, 3059
MfeI	CAATTG	5 cut(s)	582, 1773, 1803, 2343, 2433
MflI	RGATCY	1 cut(s)	1000
MhlI	GDGCHC	4 cut(s)	54, 159, 1598, 2067
MlsI	TGGCCA	2 cut(s)	243, 2957
MluNI	TGGCCA	2 cut(s)	243, 2957
Mly113I	GGCGCC	1 cut(s)	261
MlyI	GAGTC	5 cut(s)	374, 1479, 1929, 1953, 2134
MmeI	TCCRAC	4 cut(s)	693, 1435, 2040, 2243
Mox20I	TGGCCA	2 cut(s)	243, 2957
Mph1103I	ATGCAT	1 cut(s)	1850
MroI	TCCGGA	2 cut(s)	54, 1420
MroXI	GAANNNNTTC	3 cut(s)	506, 1896, 3242
MscI	TGGCCA	2 cut(s)	243, 2957
MseI	TTAA	8 cut(s)	609, 840, 1851, 2121, 2418, 2742, 2813, 3256
MslI	CAYNNNNRTG	5 cut(s)	303, 459, 1164, 2133, 2511
Msp20I	TGGCCA	2 cut(s)	243, 2957
MspA1I	CMGCKG	7 cut(s)	47, 533, 559, 1247, 1829, 2105, 2600
MspI	CCGG	9 cut(s)	55, 100, 258, 1421, 1956, 2735, 2853, 3108, 3129
MunI	CAATTG	5 cut(s)	582, 1773, 1803, 2343, 2433
Mva1269I	GAATGC	3 cut(s)	1335, 2000, 2083
MvaI	CCWGG	6 cut(s)	484, 1546, 1964, 2006, 2527, 2763
MvnI	CGCG	4 cut(s)	19, 41, 180, 731
NarI	GGCGCC	1 cut(s)	261
NciI	CCSGG	5 cut(s)	100, 101, 1956, 2735, 2736
NcoI	CCATGG	3 cut(s)	238, 739, 2279
NheI	GCTAGC	1 cut(s)	137
NmeAIII	GCCGAG	2 cut(s)	178, 2021
NmuCI	GTSAC	3 cut(s)	2267, 2491, 2987
NruI	TCGCGA	1 cut(s)	19
NsiI	ATGCAT	1 cut(s)	1850
NspI	RCATGY	3 cut(s)	939, 1848, 2034
NspV	TTCGAA	1 cut(s)	470
PaeI	GCATGC	2 cut(s)	1848, 2034
PagI	TCATGA	2 cut(s)	1492, 1609
PaqCI	CACCTGC	1 cut(s)	1282
PasI	CCCWGGG	1 cut(s)	483
PctI	GAATGC	3 cut(s)	1335, 2000, 2083
PdmI	GAANNNNTTC	3 cut(s)	506, 1896, 3242
PfeI	GAWTC	8 cut(s)	467, 1295, 1310, 1410, 1572, 1833, 3087, 3206
PflFI	GACNNNGTC	1 cut(s)	119
PfoI	TCCNGGA	2 cut(s)	1954, 1962
PinAI	ACCGGT	1 cut(s)	2852
PleI	GAGTC	5 cut(s)	373, 1478, 1928, 1953, 2134
PluTI	GGCGCC	1 cut(s)	264
PpsI	GAGTC	5 cut(s)	373, 1478, 1928, 1953, 2134
Ppu21I	YACGTR	1 cut(s)	2054
PpuMI	RGGWCCY	2 cut(s)	478, 2422
PshBI	ATTAAT	1 cut(s)	1851
Psp124BI	GAGCTC	2 cut(s)	159, 1598
Psp5II	RGGWCCY	2 cut(s)	478, 2422
Psp6I	CCWGG	6 cut(s)	482, 1544, 1962, 2004, 2525, 2761
PspEI	GGTNACC	1 cut(s)	2893
PspFI	CCCAGC	2 cut(s)	1213, 2144
PspGI	CCWGG	6 cut(s)	482, 1544, 1962, 2004, 2525, 2761
PspPI	GGNCC	6 cut(s)	193, 313, 478, 548, 1867, 2422
PspPPI	RGGWCCY	2 cut(s)	478, 2422
PstI	CTGCAG	4 cut(s)	1396, 2538, 2605, 3059
PstNI	CAGNNNCTG	1 cut(s)	2087
PsuI	RGATCY	1 cut(s)	1000
PsyI	GACNNNGTC	1 cut(s)	119
PvuII	CAGCTG	5 cut(s)	47, 533, 1829, 2105, 2600
RruI	TCGCGA	1 cut(s)	19
RsaI	GTAC	7 cut(s)	68, 648, 1020, 1606, 2559, 2749, 3101
RsaNI	GTAC	7 cut(s)	67, 647, 1019, 1605, 2558, 2748, 3100
RseI	CAYNNNNRTG	5 cut(s)	303, 459, 1164, 2133, 2511
SacI	GAGCTC	2 cut(s)	159, 1598
SaqAI	TTAA	8 cut(s)	609, 840, 1851, 2121, 2418, 2742, 2813, 3256
Sau96I	GGNCC	6 cut(s)	193, 313, 478, 548, 1867, 2422
ScaI	AGTACT	1 cut(s)	3101
SchI	GAGTC	5 cut(s)	374, 1479, 1929, 1953, 2134
SduI	GDGCHC	4 cut(s)	54, 159, 1598, 2067
SfcI	CTRYAG	4 cut(s)	1392, 2534, 2601, 3055
SfoI	GGCGCC	1 cut(s)	262
SfuI	TTCGAA	1 cut(s)	470
SinI	GGWCC	2 cut(s)	478, 2422
SmaI	CCCGGG	2 cut(s)	101, 2736
SmiMI	CAYNNNNRTG	5 cut(s)	303, 459, 1164, 2133, 2511
SmlI	CTYRAG	1 cut(s)	1693
SmoI	CTYRAG	1 cut(s)	1693
SphI	GCATGC	2 cut(s)	1848, 2034
SspDI	GGCGCC	1 cut(s)	260
SspI	AATATT	2 cut(s)	627, 1638
SspMI	CTAG	6 cut(s)	138, 822, 1260, 1451, 2586, 3017
SstI	GAGCTC	2 cut(s)	159, 1598
StyI	CCWWGG	6 cut(s)	238, 739, 1503, 2279, 3001, 3159
TaaI	ACNGT	4 cut(s)	305, 709, 2811, 2987
TaiI	ACGT	4 cut(s)	648, 2056, 3222, 3243
TaqII	GACCGA	1 cut(s)	1178
TatI	WGTACW	2 cut(s)	2747, 3099
TauI	GCSGC	6 cut(s)	44, 137, 198, 206, 266, 1872
TfiI	GAWTC	8 cut(s)	467, 1295, 1310, 1410, 1572, 1833, 3087, 3206
Tru1I	TTAA	8 cut(s)	609, 840, 1851, 2121, 2418, 2742, 2813, 3256
Tru9I	TTAA	8 cut(s)	609, 840, 1851, 2121, 2418, 2742, 2813, 3256
TscAI	CASTG	4 cut(s)	889, 1208, 1468, 2490
TseFI	GTSAC	3 cut(s)	2267, 2491, 2987
Tsp45I	GTSAC	3 cut(s)	2267, 2491, 2987
TspMI	CCCGGG	2 cut(s)	99, 2734
TspRI	CASTG	4 cut(s)	889, 1208, 1468, 2490
Tth111I	GACNNNGTC	1 cut(s)	119
VpaK11BI	GGWCC	2 cut(s)	478, 2422
VspI	ATTAAT	1 cut(s)	1851
XapI	RAATTY	6 cut(s)	11, 1399, 1627, 1705, 1937, 3211
XceI	RCATGY	3 cut(s)	939, 1848, 2034
XmaI	CCCGGG	2 cut(s)	99, 2734
XmiI	GTMKAC	2 cut(s)	828, 3228
XmnI	GAANNNNTTC	3 cut(s)	506, 1896, 3242
XspI	CTAG	6 cut(s)	138, 822, 1260, 1451, 2586, 3017
ZraI	GACGTC	1 cut(s)	3220
ZrmI	AGTACT	1 cut(s)	3101
Zsp2I	ATGCAT	1 cut(s)	1850

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.