MD07G1190200.v1.1

Protein-tyrosine-phosphatase MKP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012651
Physical Location & Seq
Forward (+)
27052759 .. 27057611
4853 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1190200.v1.1.491

Sequence Viewer

Length: 2541 bp
ATGGTGAGTAAGGAGGATGCCTCCGCTACTTCACGGCCACCGGCCCAGCTTTCGAGCTCTCGGAAGATGTTCTGGCGCTCAGCGTCGTGGTCTGCTTCACGGACCAACCCTGAGACTGAGGAAAGAGACATAGCAGATCCAAATGTCATTGTAGGAAACAGTGTCCAAAACCATCGTAGGTTTCCTGTACCTTTAACCCCTCGGTCCCAGCAGAATAGCAAGGCTAAGTCTGGTTTGCCCCCCTTGCAATTGCCCATTGCTCGGCGTAGCTTAGATGAGTGGCCCAAGGCGGGTTCAGACGACATTGGTGAGTGGCCACAACCTCCTACCCCGAGTGGCAGAAGTGGTGGGGAGAGGTTGAAGCTTGATTTGTCAACGATTCAGCGAAACCCGGAAAAGAATTGTGGGCTTGTGAGGAGGGATAAGATTGCTTTCTTTGATAAAGAGTGTTCCAAAGTGGCTGAACACATTTATGTTGGCGGAGATGCTGTTGCAAGGGACAGGGACATACTTAAGCAGAATGGGATCACACATGTTCTGAATTGTGTGGGTTTTGTTTGTCCCGAGTATTTCAAGGCGGATTTTGTGTATAGAACTCTATGGTTGCAAGATAGTCCGACTGAAGATATTACAAGTATTCTATATGATGTTTTTGATTACTTTGAAGATGTGAGGGAACAAGGTGGAAGAGTTTTGGTTCATTGTTGTCAAGGTGTGTCTCGGTCCACATCATTGGTGATTGCGTATCTTATGTGGAGGGAAGGACAGAGTTTTGATGATGCATTTCAATATGTGAAGGCAGCAAGAGGTATTGCTGATCCAAATATGGGTTTTGCTTGTCAGTTATTGCAGTGCCAAAAGAGGGTACATGCTTTCCCTCTTAGCCCGAGTTCATTATTGCGGGTGTACAGAATTGCCCCCCATTCACCATATGATCCCTTGCATCTTGTTCCAAAAATGTTGAATGATCCTTCGCCATCTGCCCTGGATTCTAGAGGTGCATTTATTGTTCATATACCTTCTGCCATATATGTTTGGATTGGTAAGAATTGTGAGGCCATCATGGAAAAGGATGCAAGAGGGGCAGTTTGTCAGATCGTTCGGTATGAGAGAGTGCAAGGTCCAATAACAATAATCAAGGAAGGGGAAGAACCAGCTTATTTTTGGGATGCTTTTTCAAACATTTTACCTTTGATGGATAGGTCTGGCAATGAAGGGGAGGCTGGGGAGTCAATAGTTAAGATTCGCCCGGGTGAAAGGAAATCAGACATATATAATATTGATTTTGAAATTTTCCAGAAAGCTATTAGTGGTGGTATTGTACCTCCATTTGCATCCTCAGAGAACGAACACGAAACCCATCTTCCTGCTAGAGAGAGCAGCTGGAGTGCCCTCAGGCGTAAGTTTGCCTCTGGCAATATGAAGGACTTTGTGTCAGCACCCAGGATATCCCTCTCCAGGGTCTATTCAGATTCTATGATGTTAGTTCATTCAGCTAACAATTCATCATCACCTTTATCAGCGTCCTCTTCGTCTTCAGCATCCTCTTCTTCTTCACCTCCATATCTTTCACCAGATTCCATCTCTTCTGAATCAAGCACTAATTCAAAGTACTTTTCAGAATCCTCTACGGATTCACCATTTGCAGCTTCATGCTCTCTTCCAGTATTTTCAACATCGTCAGATGATTCTGACATTTCTCTCCTCTCAGCTAAATCATCAGATCAGCCCATGTCTAACACTCTAGAAAATGTTGTTTCCAGTTGTAGTTCACAGCCCTATTCTAGATCAACCTCATTACCATCTAAAAAAATTTCTTCGTCGCTTGCAGAGCGCAGAGGTAGTTTGTCTCTTAAATTACCTGTAATGAGTGATGAGATGAGGCTGATGAGTACGACATCAAAATTTCTTCCCAGTAAAGAAGATGGTGTTAGGATAAATGACAGTACTTGTTCTGTAGGCCATGTAGATAATATTGACAATGCTGTAGAGTTGAAGGATGATGTTCAAAATGAAGGGGGAGATTCAACACATCAATGCAACAAATCACCACATTGGGGTGATAGTATTGGCTTATGCCAGATGGAGGCTTCTTTTATTAAACATTCCACTGAGGCTTGGAAACCTTTGGGTGAAGGTACAAAATTCTCTGCCTCAAAGGAGATTGTGGAAAGTTGTCCAGCACAATGCAATTTTATGCAACCTTTTGTGTGCCACTGGCCCAGTTTAGAGAAGATAACAGCATTTGGTGTGAGAGAATTAGATTCTAAAGCTGCTTACACTATTTTCTCACCAAACACAGGCTTTGGAAAAAGTGAAGACAGGGTTTTGTACCTCTGGGTGGGGAAATTTTTCGACAATGATAAGTTTTCCATTCAATTAGATAGTGGCAGAGAGAGAACTGATGTAGAAAAGACTGACTGGGATCAAGTTGGTTTTCATGTTCTTATCCAAATGGGTCTGCCAAAGGATACAATCATTAAGATTGTTAAAGAAAATGAAGAACCAGTGGAGTTTCTTGAATTATTGAGCCGGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000188 GO:0001704 GO:0001706 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0004725 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006464 GO:0006469 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0007369 GO:0007492 GO:0008138 GO:0008150 GO:0008152 GO:0008330 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009651 GO:0009653 GO:0009790 GO:0009888 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010224 GO:0010225 GO:0010563 GO:0010605 GO:0010646 GO:0010648 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0017017 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033549 GO:0033673 GO:0035335 GO:0035970 GO:0036211 GO:0042325 GO:0042326 GO:0042578 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043405 GO:0043407 GO:0043408 GO:0043409 GO:0043412 GO:0043549 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045859 GO:0045936 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048598 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051338 GO:0051348 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0070372 GO:0070373 GO:0071704 GO:0071900 GO:0071901 GO:0080090 GO:0140096 GO:1901564 GO:1902531 GO:1902532 GO:1990439
KEGG Pathways
Metabolic & Signaling
ko04010 ko04726 ko05418 map04010 map04726 map05418
Pfam Domains
Protein Families
DSPc

Protein Analysis

847

Amino Acids

93.77

Weight (kDa)

5.71

Isoelectric Point (pI)

53.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSPc PF00782 157 - 287 1.5e-26 Dual specificity phosphatase, catalytic domain
MPK1_gelsolin_C PF25466 739 - 843 2.5e-37 MPK1 gelsolin domain C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 24, 290, 480, 578, 901
AclWI GGATC 6 cut(s) 131, 533, 812, 929, 962, 2433
AcoI YGGCCR 2 cut(s) 35, 314
AcsI RAATTY 5 cut(s) 1290, 1812, 1904, 2144, 2348
AcuI CTGAAG 2 cut(s) 642, 1521
AfaI GTAC 9 cut(s) 189, 867, 908, 1323, 1613, 1894, 1948, 2140, 2333
AflII CTTAAG 1 cut(s) 512
AflIII ACRYGT 1 cut(s) 532
AhdI GACNNNNNGTC 1 cut(s) 1432
AjnI CCWGG 3 cut(s) 984, 1442, 1457
Alw21I GWGCWC 1 cut(s) 59
Alw26I GTCTC 4 cut(s) 107, 120, 723, 1854
AlwI GGATC 6 cut(s) 131, 533, 812, 929, 962, 2433
Ama87I CYCGRG 4 cut(s) 331, 563, 886, 1249
AoxI GGCC 7 cut(s) 35, 42, 281, 314, 1056, 1960, 2219
ApeKI GCWGC 4 cut(s) 800, 1380, 1646, 2273
ApoI RAATTY 5 cut(s) 1290, 1812, 1904, 2144, 2348
Asp700I GAANNNNTTC 2 cut(s) 68, 2351
AspLEI GCGC 2 cut(s) 78, 1836
AspS9I GGNCC 7 cut(s) 43, 102, 204, 282, 723, 1121, 2220
AsuC2I CCSGG 3 cut(s) 392, 1250, 1251
AvaI CYCGRG 4 cut(s) 331, 563, 886, 1249
AvaII GGWCC 4 cut(s) 102, 204, 723, 1121
AxyI CCTNAGG 1 cut(s) 1394
BaeGI GKGCMC 1 cut(s) 1393
BalI TGGCCA 1 cut(s) 316
BanII GRGCYC 1 cut(s) 59
BbsI GAAGAC 2 cut(s) 1527, 2325
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 4 cut(s) 812, 1392, 1658, 2260
BccI CCATC 9 cut(s) 180, 985, 1067, 1189, 1368, 1589, 1810, 1919, 2077
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 3 cut(s) 986, 1444, 1459
BciVI GTATCC 1 cut(s) 2464
BcnI CCSGG 3 cut(s) 392, 1250, 1251
BcoDI GTCTC 4 cut(s) 107, 120, 723, 1854
BfaI CTAG 4 cut(s) 993, 1371, 1745, 1785
BfmI CTRYAG 2 cut(s) 1956, 1986
BfoI RGCGCY 1 cut(s) 79
BfrI CTTAAG 1 cut(s) 512
BfuI GTATCC 1 cut(s) 2464
BisI GCNGC 4 cut(s) 801, 1381, 1647, 2274
BlpI GCTNAGC 1 cut(s) 79
BlsI GCNGC 4 cut(s) 802, 1382, 1648, 2275
BmcAI AGTACT 2 cut(s) 1613, 1948
Bme1390I CCNGG 6 cut(s) 392, 986, 1250, 1251, 1444, 1459
Bme18I GGWCC 4 cut(s) 102, 204, 723, 1121
BmeRI GACNNNNNGTC 1 cut(s) 1432
BmeT110I CYCGRG 4 cut(s) 331, 563, 886, 1249
BmgT120I GGNCC 7 cut(s) 43, 102, 204, 282, 723, 1121, 2220
BmiI GGNNCC 1 cut(s) 206
BmrFI CCNGG 6 cut(s) 392, 986, 1250, 1251, 1444, 1459
BmrI ACTGGG 3 cut(s) 1908, 2217, 2431
BmsI GCATC 8 cut(s) 7, 475, 769, 952, 1063, 1159, 1343, 1550
BmuI ACTGGG 3 cut(s) 1908, 2217, 2431
BpiI GAAGAC 2 cut(s) 1527, 2325
BplI GAGNNNNNCTC 1 cut(s) 37
BpmI CTGGAG 2 cut(s) 1405, 1441
Bpu1102I GCTNAGC 1 cut(s) 79
BpuMI CCSGG 3 cut(s) 392, 1250, 1251
BsaJI CCNNGG 6 cut(s) 200, 285, 984, 1249, 1442, 1458
BsaXI ACNNNNNCTCC 2 cut(s) 474, 504
Bse118I RCCGGY 2 cut(s) 40, 2532
Bse1I ACTGG 7 cut(s) 1664, 1761, 1914, 2222, 2223, 2426, 2507
Bse21I CCTNAGG 1 cut(s) 1394
Bse3DI GCAATG 2 cut(s) 255, 1216
BseBI CCWGG 3 cut(s) 986, 1444, 1459
BseDI CCNNGG 6 cut(s) 200, 285, 984, 1249, 1442, 1458
BseGI GGATG 6 cut(s) 22, 1078, 1174, 1334, 1541, 2005
BseMI GCAATG 2 cut(s) 255, 1216
BseMII CTCAG 7 cut(s) 93, 102, 108, 1353, 1408, 1722, 2103
BseNI ACTGG 7 cut(s) 1664, 1761, 1914, 2222, 2223, 2426, 2507
BseRI GAGGAG 2 cut(s) 430, 1694
BseSI GKGCMC 1 cut(s) 1393
BseXI GCAGC 4 cut(s) 812, 1392, 1658, 2260
BseYI CCCAGC 3 cut(s) 45, 207, 1223
BshFI GGCC 7 cut(s) 37, 44, 283, 316, 1058, 1962, 2221
BsiHKAI GWGCWC 1 cut(s) 59
BsiHKCI CYCGRG 4 cut(s) 331, 563, 886, 1249
BsiSI CCGG 4 cut(s) 41, 392, 1250, 2533
BslFI GGGAC 4 cut(s) 190, 512, 518, 546
BsmAI GTCTC 4 cut(s) 107, 120, 723, 1854
BsmFI GGGAC 4 cut(s) 190, 512, 518, 546
BsnI GGCC 7 cut(s) 37, 44, 283, 316, 1058, 1962, 2221
BsoBI CYCGRG 4 cut(s) 331, 563, 886, 1249
Bsp1286I GDGCHC 2 cut(s) 59, 1393
Bsp1407I TGTACA 1 cut(s) 906
Bsp143I GATC 9 cut(s) 136, 525, 817, 934, 967, 1095, 1723, 1787, 2425
Bsp1720I GCTNAGC 1 cut(s) 79
BspACI CCGC 5 cut(s) 24, 290, 480, 578, 901
BspANI GGCC 7 cut(s) 37, 44, 283, 316, 1058, 1962, 2221
BspCNI CTCAG 7 cut(s) 92, 103, 109, 1352, 1407, 1721, 2104
BspLI GGNNCC 1 cut(s) 206
BspPI GGATC 6 cut(s) 131, 533, 812, 929, 962, 2433
BspTI CTTAAG 1 cut(s) 512
BsrDI GCAATG 2 cut(s) 255, 1216
BsrFI RCCGGY 2 cut(s) 40, 2532
BsrGI TGTACA 1 cut(s) 906
BsrI ACTGG 7 cut(s) 1664, 1761, 1914, 2222, 2223, 2426, 2507
BssAI RCCGGY 2 cut(s) 40, 2532
BssECI CCNNGG 6 cut(s) 200, 285, 984, 1249, 1442, 1458
BssMI GATC 9 cut(s) 136, 525, 817, 934, 967, 1095, 1723, 1787, 2425
BssT1I CCWWGG 1 cut(s) 285
Bst2UI CCWGG 3 cut(s) 986, 1444, 1459
Bst4CI ACNGT 2 cut(s) 161, 1946
Bst6I CTCTTC 5 cut(s) 682, 1534, 1552, 1591, 1665
BstAFI CTTAAG 1 cut(s) 512
BstAUI TGTACA 1 cut(s) 906
BstC8I GCNNGC 1 cut(s) 1827
BstF5I GGATG 6 cut(s) 22, 1078, 1174, 1334, 1541, 2005
BstH2I RGCGCY 1 cut(s) 79
BstHHI GCGC 2 cut(s) 78, 1836
BstKTI GATC 9 cut(s) 139, 528, 820, 937, 970, 1098, 1726, 1790, 2428
BstMAI GTCTC 4 cut(s) 107, 120, 723, 1854
BstMBI GATC 9 cut(s) 136, 525, 817, 934, 967, 1095, 1723, 1787, 2425
BstMWI GCNNNNNNNGC 3 cut(s) 244, 1082, 1831
BstNI CCWGG 3 cut(s) 986, 1444, 1459
BstNSI RCATGY 2 cut(s) 536, 872
BstSCI CCNGG 6 cut(s) 390, 984, 1248, 1249, 1442, 1457
BstSFI CTRYAG 2 cut(s) 1956, 1986
BstSLI GKGCMC 1 cut(s) 1393
BstV1I GCAGC 4 cut(s) 812, 1392, 1658, 2260
BstV2I GAAGAC 2 cut(s) 1527, 2325
BstX2I RGATCY 1 cut(s) 136
BstXI CCANNNNNNTGG 1 cut(s) 733
BstYI RGATCY 1 cut(s) 136
Bsu36I CCTNAGG 1 cut(s) 1394
BsuI GTATCC 1 cut(s) 2464
BsuRI GGCC 7 cut(s) 37, 44, 283, 316, 1058, 1962, 2221
BtsCI GGATG 6 cut(s) 22, 1078, 1174, 1334, 1541, 2005
BtsI GCAGTG 1 cut(s) 857
BtsIMutI CAGTG 5 cut(s) 166, 857, 2109, 2215, 2514
Cac8I GCNNGC 1 cut(s) 1827
CfoI GCGC 2 cut(s) 78, 1836
Cfr10I RCCGGY 2 cut(s) 40, 2532
Cfr13I GGNCC 7 cut(s) 43, 102, 204, 282, 723, 1121, 2220
Cfr9I CCCGGG 1 cut(s) 1249
CseI GACGC 2 cut(s) 72, 1512
Csp6I GTAC 9 cut(s) 188, 866, 907, 1322, 1612, 1893, 1947, 2139, 2332
CviAII CATG 7 cut(s) 533, 869, 1063, 1653, 1732, 1964, 2441
CviQI GTAC 9 cut(s) 188, 866, 907, 1322, 1612, 1893, 1947, 2139, 2332
DpnI GATC 9 cut(s) 138, 527, 819, 936, 969, 1097, 1725, 1789, 2427
DpnII GATC 9 cut(s) 136, 525, 817, 934, 967, 1095, 1723, 1787, 2425
DriI GACNNNNNGTC 1 cut(s) 1432
EaeI YGGCCR 2 cut(s) 35, 314
Eam1104I CTCTTC 5 cut(s) 682, 1534, 1552, 1591, 1665
Eam1105I GACNNNNNGTC 1 cut(s) 1432
EarI CTCTTC 5 cut(s) 682, 1534, 1552, 1591, 1665
EciI GGCGGA 2 cut(s) 495, 593
Ecl136II GAGCTC 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 59
Eco32I GATATC 1 cut(s) 1449
Eco47I GGWCC 4 cut(s) 102, 204, 723, 1121
Eco53kI GAGCTC 1 cut(s) 57
Eco57I CTGAAG 2 cut(s) 642, 1521
Eco81I CCTNAGG 1 cut(s) 1394
Eco88I CYCGRG 4 cut(s) 331, 563, 886, 1249
EcoICRI GAGCTC 1 cut(s) 57
EcoRII CCWGG 3 cut(s) 984, 1442, 1457
EcoRV GATATC 1 cut(s) 1449
EcoT14I CCWWGG 1 cut(s) 285
EcoT22I ATGCAT 1 cut(s) 784
EcoT38I GRGCYC 1 cut(s) 59
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 7 cut(s) 536, 872, 1066, 1656, 1735, 1967, 2444
FaqI GGGAC 4 cut(s) 190, 512, 518, 546
FatI CATG 7 cut(s) 532, 868, 1062, 1652, 1731, 1963, 2440
FauI CCCGC 2 cut(s) 283, 894
FauNDI CATATG 1 cut(s) 931
Fnu4HI GCNGC 4 cut(s) 801, 1381, 1647, 2274
FokI GGATG 6 cut(s) 29, 1085, 1181, 1321, 1528, 2012
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 4 cut(s) 801, 1381, 1647, 2274
FspBI CTAG 4 cut(s) 993, 1371, 1745, 1785
GlaI GCGC 2 cut(s) 77, 1835
GluI GCNGC 4 cut(s) 801, 1381, 1647, 2274
GsaI CCCAGC 3 cut(s) 49, 211, 1227
GsuI CTGGAG 2 cut(s) 1405, 1441
HaeII RGCGCY 1 cut(s) 79
HaeIII GGCC 7 cut(s) 37, 44, 283, 316, 1058, 1962, 2221
HapII CCGG 4 cut(s) 41, 392, 1250, 2533
HgaI GACGC 2 cut(s) 72, 1512
HhaI GCGC 2 cut(s) 78, 1836
Hin1II CATG 7 cut(s) 536, 872, 1066, 1656, 1735, 1967, 2444
Hin6I GCGC 2 cut(s) 76, 1834
HinP1I GCGC 2 cut(s) 76, 1834
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HindIII AAGCTT 1 cut(s) 362
HpaII CCGG 4 cut(s) 41, 392, 1250, 2533
Hpy166II GTNNAC 4 cut(s) 375, 726, 907, 1772
Hpy188III TCNNGA 6 cut(s) 563, 993, 1297, 1745, 1785, 2519
Hpy8I GTNNAC 4 cut(s) 375, 726, 907, 1772
Hpy99I CGWCG 2 cut(s) 88, 1825
HpyCH4III ACNGT 2 cut(s) 161, 1946
HpyF10VI GCNNNNNNNGC 3 cut(s) 244, 1082, 1831
Hsp92II CATG 7 cut(s) 536, 872, 1066, 1656, 1735, 1967, 2444
HspAI GCGC 2 cut(s) 76, 1834
Kzo9I GATC 9 cut(s) 136, 525, 817, 934, 967, 1095, 1723, 1787, 2425
Lsp1109I GCAGC 4 cut(s) 812, 1392, 1658, 2260
LweI GCATC 8 cut(s) 7, 475, 769, 952, 1063, 1159, 1343, 1550
MaeI CTAG 4 cut(s) 993, 1371, 1745, 1785
MalI GATC 9 cut(s) 138, 527, 819, 936, 969, 1097, 1725, 1789, 2427
MboI GATC 9 cut(s) 136, 525, 817, 934, 967, 1095, 1723, 1787, 2425
MfeI CAATTG 1 cut(s) 248
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 2 cut(s) 59, 1393
MlsI TGGCCA 1 cut(s) 316
MluNI TGGCCA 1 cut(s) 316
MlyI GAGTC 1 cut(s) 1238
MmeI TCCRAC 1 cut(s) 641
Mox20I TGGCCA 1 cut(s) 316
Mph1103I ATGCAT 1 cut(s) 784
MroXI GAANNNNTTC 2 cut(s) 68, 2351
MscI TGGCCA 1 cut(s) 316
MseI TTAA 7 cut(s) 194, 513, 1239, 1854, 2100, 2481, 2490
MslI CAYNNNNRTG 3 cut(s) 471, 2035, 2058
Msp20I TGGCCA 1 cut(s) 316
MspA1I CMGCKG 1 cut(s) 1383
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 4 cut(s) 41, 392, 1250, 2533
MspR9I CCNGG 6 cut(s) 392, 986, 1250, 1251, 1444, 1459
MunI CAATTG 1 cut(s) 248
MvaI CCWGG 3 cut(s) 986, 1444, 1459
MwoI GCNNNNNNNGC 3 cut(s) 244, 1082, 1831
NciI CCSGG 3 cut(s) 392, 1250, 1251
NdeI CATATG 1 cut(s) 931
NdeII GATC 9 cut(s) 136, 525, 817, 934, 967, 1095, 1723, 1787, 2425
NlaIII CATG 7 cut(s) 536, 872, 1066, 1656, 1735, 1967, 2444
NlaIV GGNNCC 1 cut(s) 206
NmeAIII GCCGAG 1 cut(s) 241
NsiI ATGCAT 1 cut(s) 784
NspI RCATGY 2 cut(s) 536, 872
PciI ACATGT 1 cut(s) 532
PdmI GAANNNNTTC 2 cut(s) 68, 2351
PkrI GCNGC 4 cut(s) 802, 1382, 1648, 2275
PleI GAGTC 1 cut(s) 1237
PpsI GAGTC 1 cut(s) 1237
PscI ACATGT 1 cut(s) 532
Psp124BI GAGCTC 1 cut(s) 59
Psp6I CCWGG 3 cut(s) 984, 1442, 1457
PspFI CCCAGC 3 cut(s) 45, 207, 1223
PspGI CCWGG 3 cut(s) 984, 1442, 1457
PspN4I GGNNCC 1 cut(s) 206
PspPI GGNCC 7 cut(s) 43, 102, 204, 282, 723, 1121, 2220
PsuI RGATCY 1 cut(s) 136
PvuII CAGCTG 1 cut(s) 1383
RsaI GTAC 9 cut(s) 189, 867, 908, 1323, 1613, 1894, 1948, 2140, 2333
RsaNI GTAC 9 cut(s) 188, 866, 907, 1322, 1612, 1893, 1947, 2139, 2332
RseI CAYNNNNRTG 3 cut(s) 471, 2035, 2058
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 7 cut(s) 194, 513, 1239, 1854, 2100, 2481, 2490
SatI GCNGC 4 cut(s) 801, 1381, 1647, 2274
Sau3AI GATC 9 cut(s) 136, 525, 817, 934, 967, 1095, 1723, 1787, 2425
Sau96I GGNCC 7 cut(s) 43, 102, 204, 282, 723, 1121, 2220
ScaI AGTACT 2 cut(s) 1613, 1948
SchI GAGTC 1 cut(s) 1238
ScrFI CCNGG 6 cut(s) 392, 986, 1250, 1251, 1444, 1459
SduI GDGCHC 2 cut(s) 59, 1393
SfaNI GCATC 8 cut(s) 7, 475, 769, 952, 1063, 1159, 1343, 1550
SfcI CTRYAG 2 cut(s) 1956, 1986
SinI GGWCC 4 cut(s) 102, 204, 723, 1121
SmaI CCCGGG 1 cut(s) 1251
SmiMI CAYNNNNRTG 3 cut(s) 471, 2035, 2058
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
SsiI CCGC 5 cut(s) 24, 290, 480, 578, 901
SspI AATATT 2 cut(s) 1279, 1975
SspMI CTAG 4 cut(s) 993, 1371, 1745, 1785
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 6 cut(s) 390, 984, 1248, 1249, 1442, 1457
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 2 cut(s) 161, 1946
TaqI TCGA 2 cut(s) 53, 2355
TaqII GACCGA 2 cut(s) 192, 711
TatI WGTACW 3 cut(s) 906, 1611, 1946
Tru1I TTAA 7 cut(s) 194, 513, 1239, 1854, 2100, 2481, 2490
Tru9I TTAA 7 cut(s) 194, 513, 1239, 1854, 2100, 2481, 2490
TscAI CASTG 5 cut(s) 166, 857, 2116, 2222, 2514
TseI GCWGC 4 cut(s) 800, 1380, 1646, 2273
TspGWI ACGGA 2 cut(s) 115, 1646
TspMI CCCGGG 1 cut(s) 1249
TspRI CASTG 5 cut(s) 166, 857, 2116, 2222, 2514
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 4 cut(s) 102, 204, 723, 1121
XapI RAATTY 5 cut(s) 1290, 1812, 1904, 2144, 2348
XbaI TCTAGA 3 cut(s) 992, 1744, 1784
XceI RCATGY 2 cut(s) 536, 872
XcmI CCANNNNNNNNNTGG 1 cut(s) 1161
XmaI CCCGGG 1 cut(s) 1249
XmnI GAANNNNTTC 2 cut(s) 68, 2351
XspI CTAG 4 cut(s) 993, 1371, 1745, 1785
ZrmI AGTACT 2 cut(s) 1613, 1948
Zsp2I ATGCAT 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.