pycom01g14740

Protein-tyrosine-phosphatase MKP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012651
Physical Location & Seq
Forward (+)
14826382 .. 14829561
3180 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g14740.1

Sequence Viewer

Length: 2523 bp
ATGGTGAGTAAGGAGGATGCCTCCGCTACTTCACGGCCTCCGGCCCAGCTTTCGAGCTCTCGGAAGCTGTTCTGGCGCTCAGCGTCGTGGTCTGCTTCCCGGACCAATCCTGAGACTGAGGAAAGAGACCTAGCAGATCCAAATGCCATTGTAGGAAACAGTGTCCAAAACCATCGTAGGTTTCCTGTACCCTTAACCCCTCGGTCCCAGCAGAATAGCAAGGCTAGGTCTGGTTTGCCCCCCTTGCAGTTGCCCATTGCTCGGCGTAGCTTAGATGAGTGGCCCAAGGCAGGTTCAGATGATATTGGTGAGTGGCCACAACCTCCTACCCCAAGTGGAAGAAGCAGTGGGGAGAGGTTGAAGCTTGATTTGTCAGCGATTCAGCGAAATCCTGAAAAGAATGGCGGGCTTGTGAGGAGGGATAAGATTGCTTTCTTTGATAAAGAGTGTTCCAAAGTGGCTGAACACATTTATCTTGGGGGAGATGCTGTTGCAAGGGATAGGGACATACTTAAGCAGAGCGGGATCACCCATGTTCTGAATTGTGTGGGTTTTGTTTGTCCCGAGTATTTCAAGGGGGATTTTGTGTACAGAACTCTGTGGTTGCAGGATAGTCCGACTGAGGATATTACAAGTATTTTATATGATGTTTTTGATTACTTTGAAGACGTTAGGGAACAAGGTGGAAGAGTTTTGGTTCATTGTTGTCAAGGTGTGTCTCGGTCCACATCGTTGGTGATTGCGTATCTTATGTGGAGAGAAGGACAGAGTTTTGATGATGCGTTTCAGTATGTGAAAGCAGCAAGAGGTATTGCTGATCCAAATATGGGTTTTGCTTGTCAGTTGTTACAGTGCCAAAAGAGGGTCCATGCTTTCCCTCTTAGCCCGAGTTCACTATTGAGGATGTACAGAATTGCCCCACATTCATCATATGATCGATTGCATCTTGTTCCAAAAATGTTGAATGATCCTTCGCAGTCTGCCTTGGATTCTAGAGGTGCATTTATTGTTCATATACCTTCTGCCATATATTTTTGGATTGGTAAGAATTGTGAGGCCATCATGGAAAGGGATGCAAGAGGGGCTGTTTGTCAGATTGTTCGGTATGAGAGAGTGCAAGGTCCAATAACAATAATCAAGGAAGGGGAAGAACCAGCGTACTTTTGGGATGCTTTCTCGAACATTTTACCTTTGATGGATAAGTCTGGCAATGAAGGGGACGTTGGGGAGTCAGTAGTTAAGATTCGCCCAGGTGAAAGAAAAACAGACATGTATAATATTGATTATGAAATTTTCCAGAAAGCTATTAGTGGTGGTTTTGTACCTCCAATTGCATCCTCAGAGAACGAACACGAAACCCATCTTCCTGCTAGAGAGAGCAGCTGGAGTGCTCTCAGGCGTAAGTTTGCCTCTGAAAGTATGAAGGACTTTGTGTCAGCACCCAGGATATCCCTCTCCAGGGTCTATTCAGATTCTATGATGTTAGTTCATTCAGCTAAAAATTCGTCGTCACCTGTATCAGCGCCGTCTTCATCTTCATCAGCTTCTTCTTCGTCACCTTCATATCTATCACCAGATTCCATTTCTTCTGAATCAAGCACTAATTCAAAGTACTTTTCAGAATCCTCTACCGATTCGCCATCTGCAGCTTCATGCTCTCTTCCAGTATCTTCAACATCGTCAGATGATTCTGATGTGTCACTCCTCTCAGCTAAATCATCTGATCAACCCATGTCTAACACACTAGAAAATGTTGTTTCCAGTTGTAGTTCACAGCCCTATTCACGATCAATCTCATTACCATCTAAAAGAATTTCATCTTCGCTTGCAAAGCGCAGAGGTAATTTGTCTCTTAAATTACCTGTCATTAGTGATGAGATGAGGCTGATGAGTCCTTCGTCAAAATTTCTTCCCAGTAAAGAAGACAGTGTTAGGATAAATGACAGTACTTTTTCGATAGGTCACGTAGATAATATTGACAATGCCTTAGAGTCGAAGGATGATGTTCAAAATGGAGGGGGAGACAAATCCCCGTGTAGGGAAAATAGTATTGACTCATGCCAGAAGGAAACTTCTTTTATCAAACATTCCACTGAGGCTTGGAACCCTTTGAAGGAAGGTACAGAATCCTCTGCCTCAAAGGAGATTGTGGAAAGTTGTCCAGCGCAATGCAATTTTATCCAACCTTTTGTGTGCCGCTGGCCCAGTTTAGAGAAGATTGCAACATTTGGTGTGAGGGAACTAGATTCTAAAGCTGCTTACACTATTTTCTCACCAAATACAGATTTTGGAAAAAGTGAAGACAGGGTTTTGTACCTCTGGGTGGGAAGATTTTTCGACAGTGATAAGTTTTCCATTCAACTAGATAGTGGAAGAGAGAGAACTGATGTAGAAGAGAGTGACTGGGATCAAGTTGGTTTTCATGTTCTTGTTCAAATGGGTCTGCCAAAGGACACAATCATTAAGATTGTTAAAGAAAATGAAGAACCAGTGGAGTTTCTTGAAATATTGAGCCGGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000188 GO:0001704 GO:0001706 GO:0001932 GO:0001933 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0004725 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006464 GO:0006469 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0007369 GO:0007492 GO:0008138 GO:0008150 GO:0008152 GO:0008330 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009651 GO:0009653 GO:0009790 GO:0009888 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010224 GO:0010225 GO:0010563 GO:0010605 GO:0010646 GO:0010648 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0017017 GO:0019220 GO:0019222 GO:0019538 GO:0023051 GO:0023057 GO:0031323 GO:0031324 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0033549 GO:0033673 GO:0035335 GO:0035970 GO:0036211 GO:0042325 GO:0042326 GO:0042578 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043405 GO:0043407 GO:0043408 GO:0043409 GO:0043412 GO:0043549 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045859 GO:0045936 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048598 GO:0048646 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051174 GO:0051246 GO:0051248 GO:0051338 GO:0051348 GO:0060255 GO:0065007 GO:0065009 GO:0070013 GO:0070372 GO:0070373 GO:0071704 GO:0071900 GO:0071901 GO:0080090 GO:0140096 GO:1901564 GO:1902531 GO:1902532 GO:1990439
KEGG Pathways
Metabolic & Signaling
ko04010 ko04726 ko05418 map04010 map04726 map05418
Pfam Domains
Protein Families
DSPc

Protein Analysis

841

Amino Acids

93.26

Weight (kDa)

5.66

Isoelectric Point (pI)

60.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSPc PF00782 157 - 287 1.2e-26 Dual specificity phosphatase, catalytic domain
MPK1_gelsolin_C PF25466 733 - 837 1.1e-37 MPK1 gelsolin domain C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 281
AccBSI CCGCTC 1 cut(s) 522
AciI CCGC 4 cut(s) 24, 405, 522, 2197
AclWI GGATC 5 cut(s) 131, 533, 812, 962, 2415
AcoI YGGCCR 1 cut(s) 314
AcsI RAATTY 4 cut(s) 1290, 1501, 1812, 1904
AfaI GTAC 9 cut(s) 189, 590, 908, 1160, 1323, 1613, 1948, 2122, 2315
AflII CTTAAG 1 cut(s) 512
AflIII ACRYGT 1 cut(s) 1269
AhdI GACNNNNNGTC 1 cut(s) 1432
AjnI CCWGG 3 cut(s) 1249, 1442, 1457
AjuI GAANNNNNNNTTGG 2 cut(s) 1206, 1238
Alw21I GWGCWC 2 cut(s) 59, 1393
Alw26I GTCTC 5 cut(s) 107, 120, 723, 1854, 2016
AlwI GGATC 5 cut(s) 131, 533, 812, 962, 2415
Ama87I CYCGRG 2 cut(s) 563, 886
AoxI GGCC 6 cut(s) 35, 42, 281, 314, 1056, 2201
ApeKI GCWGC 4 cut(s) 800, 1380, 1646, 2255
ApoI RAATTY 4 cut(s) 1290, 1501, 1812, 1904
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 4 cut(s) 78, 1525, 1836, 2167
AspS9I GGNCC 8 cut(s) 43, 102, 204, 282, 723, 865, 1121, 2202
AsuC2I CCSGG 1 cut(s) 100
AsuHPI GGTGA 9 cut(s) 16, 320, 520, 748, 1265, 1503, 1548, 1563, 2265
AvaI CYCGRG 2 cut(s) 563, 886
AvaII GGWCC 5 cut(s) 102, 204, 723, 865, 1121
BalI TGGCCA 1 cut(s) 316
BanII GRGCYC 1 cut(s) 59
BbsI GAAGAC 4 cut(s) 672, 1521, 1929, 2307
Bbv12I GWGCWC 2 cut(s) 59, 1393
BbvI GCAGC 4 cut(s) 812, 1392, 1658, 2242
BccI CCATC 6 cut(s) 180, 1067, 1189, 1368, 1648, 1810
BceAI ACGGC 2 cut(s) 50, 1510
BciT130I CCWGG 3 cut(s) 1251, 1444, 1459
BclI TGATCA 1 cut(s) 1723
BcnI CCSGG 1 cut(s) 100
BcoDI GTCTC 5 cut(s) 107, 120, 723, 1854, 2016
BfaI CTAG 7 cut(s) 131, 225, 993, 1371, 1745, 2243, 2363
BfmI CTRYAG 1 cut(s) 1644
BfoI RGCGCY 2 cut(s) 79, 1526
BfrI CTTAAG 1 cut(s) 512
BfuAI ACCTGC 1 cut(s) 281
BisI GCNGC 5 cut(s) 801, 1381, 1647, 2197, 2256
BlpI GCTNAGC 1 cut(s) 79
BlsI GCNGC 5 cut(s) 802, 1382, 1648, 2198, 2257
BmcAI AGTACT 2 cut(s) 1613, 1948
Bme1390I CCNGG 4 cut(s) 100, 1251, 1444, 1459
Bme18I GGWCC 5 cut(s) 102, 204, 723, 865, 1121
BmeRI GACNNNNNGTC 1 cut(s) 1432
BmeT110I CYCGRG 2 cut(s) 563, 886
BmgT120I GGNCC 8 cut(s) 43, 102, 204, 282, 723, 865, 1121, 2202
BmiI GGNNCC 3 cut(s) 206, 866, 2105
BmrFI CCNGG 4 cut(s) 100, 1251, 1444, 1459
BmrI ACTGGG 3 cut(s) 1908, 2199, 2413
BmsI GCATC 7 cut(s) 7, 475, 769, 952, 1063, 1159, 1343
BmuI ACTGGG 3 cut(s) 1908, 2199, 2413
BpiI GAAGAC 4 cut(s) 672, 1521, 1929, 2307
BplI GAGNNNNNCTC 1 cut(s) 37
BpmI CTGGAG 2 cut(s) 1405, 1441
Bpu1102I GCTNAGC 1 cut(s) 79
BpuMI CCSGG 1 cut(s) 100
Bsa29I ATCGAT 1 cut(s) 937
BsaAI YACGTR 1 cut(s) 1966
BsaI GGTCTC 1 cut(s) 120
BsaJI CCNNGG 6 cut(s) 200, 285, 984, 1249, 1442, 1458
BsaXI ACNNNNNCTCC 2 cut(s) 474, 504
Bse118I RCCGGY 1 cut(s) 2514
Bse1I ACTGG 6 cut(s) 1664, 1761, 1914, 2205, 2408, 2489
Bse3DI GCAATG 3 cut(s) 255, 1216, 2174
BseBI CCWGG 3 cut(s) 1251, 1444, 1459
BseCI ATCGAT 1 cut(s) 937
BseDI CCNNGG 6 cut(s) 200, 285, 984, 1249, 1442, 1458
BseGI GGATG 6 cut(s) 22, 909, 1078, 1174, 1334, 2005
BseMI GCAATG 3 cut(s) 255, 1216, 2174
BseMII CTCAG 8 cut(s) 93, 102, 108, 612, 1353, 1408, 1722, 2085
BseNI ACTGG 6 cut(s) 1664, 1761, 1914, 2205, 2408, 2489
BseRI GAGGAG 2 cut(s) 430, 1694
BseXI GCAGC 4 cut(s) 812, 1392, 1658, 2242
BseYI CCCAGC 2 cut(s) 45, 207
BshFI GGCC 6 cut(s) 37, 44, 283, 316, 1058, 2203
BshVI ATCGAT 1 cut(s) 937
BsiHKAI GWGCWC 2 cut(s) 59, 1393
BsiHKCI CYCGRG 2 cut(s) 563, 886
BsiSI CCGG 3 cut(s) 41, 100, 2515
BslFI GGGAC 4 cut(s) 190, 518, 546, 1232
BsmAI GTCTC 5 cut(s) 107, 120, 723, 1854, 2016
BsmFI GGGAC 4 cut(s) 190, 518, 546, 1232
BsnI GGCC 6 cut(s) 37, 44, 283, 316, 1058, 2203
Bso31I GGTCTC 1 cut(s) 120
BsoBI CYCGRG 2 cut(s) 563, 886
Bsp1286I GDGCHC 2 cut(s) 59, 1393
Bsp1407I TGTACA 2 cut(s) 588, 906
Bsp143I GATC 8 cut(s) 136, 525, 817, 934, 967, 1723, 1787, 2407
Bsp1720I GCTNAGC 1 cut(s) 79
BspACI CCGC 4 cut(s) 24, 405, 522, 2197
BspANI GGCC 6 cut(s) 37, 44, 283, 316, 1058, 2203
BspCNI CTCAG 8 cut(s) 92, 103, 109, 613, 1352, 1407, 1721, 2086
BspDI ATCGAT 1 cut(s) 937
BspLI GGNNCC 3 cut(s) 206, 866, 2105
BspMAI CTGCAG 1 cut(s) 1648
BspMI ACCTGC 1 cut(s) 281
BspPI GGATC 5 cut(s) 131, 533, 812, 962, 2415
BspTI CTTAAG 1 cut(s) 512
BspTNI GGTCTC 1 cut(s) 120
BsrBI CCGCTC 1 cut(s) 522
BsrDI GCAATG 3 cut(s) 255, 1216, 2174
BsrFI RCCGGY 1 cut(s) 2514
BsrGI TGTACA 2 cut(s) 588, 906
BsrI ACTGG 6 cut(s) 1664, 1761, 1914, 2205, 2408, 2489
BssAI RCCGGY 1 cut(s) 2514
BssECI CCNNGG 6 cut(s) 200, 285, 984, 1249, 1442, 1458
BssMI GATC 8 cut(s) 136, 525, 817, 934, 967, 1723, 1787, 2407
BssT1I CCWWGG 2 cut(s) 285, 984
Bst2UI CCWGG 3 cut(s) 1251, 1444, 1459
Bst4CI ACNGT 5 cut(s) 161, 852, 1928, 1946, 2342
Bst6I CTCTTC 4 cut(s) 682, 1665, 2368, 2388
BstAFI CTTAAG 1 cut(s) 512
BstAUI TGTACA 2 cut(s) 588, 906
BstBAI YACGTR 1 cut(s) 1966
BstC8I GCNNGC 3 cut(s) 407, 1827, 2201
BstF5I GGATG 6 cut(s) 22, 909, 1078, 1174, 1334, 2005
BstH2I RGCGCY 2 cut(s) 79, 1526
BstHHI GCGC 4 cut(s) 78, 1525, 1836, 2167
BstKTI GATC 8 cut(s) 139, 528, 820, 937, 970, 1726, 1790, 2410
BstMAI GTCTC 5 cut(s) 107, 120, 723, 1854, 2016
BstMBI GATC 8 cut(s) 136, 525, 817, 934, 967, 1723, 1787, 2407
BstMWI GCNNNNNNNGC 4 cut(s) 73, 244, 1082, 1831
BstNI CCWGG 3 cut(s) 1251, 1444, 1459
BstNSI RCATGY 1 cut(s) 1273
BstSCI CCNGG 4 cut(s) 98, 1249, 1442, 1457
BstSFI CTRYAG 1 cut(s) 1644
BstV1I GCAGC 4 cut(s) 812, 1392, 1658, 2242
BstV2I GAAGAC 4 cut(s) 672, 1521, 1929, 2307
BstX2I RGATCY 1 cut(s) 136
BstXI CCANNNNNNTGG 1 cut(s) 733
BstYI RGATCY 1 cut(s) 136
Bsu15I ATCGAT 1 cut(s) 937
BsuRI GGCC 6 cut(s) 37, 44, 283, 316, 1058, 2203
BsuTUI ATCGAT 1 cut(s) 937
BtsCI GGATG 6 cut(s) 22, 909, 1078, 1174, 1334, 2005
BtsI GCAGTG 1 cut(s) 352
BtsIMutI CAGTG 7 cut(s) 166, 352, 857, 1933, 2091, 2347, 2496
BveI ACCTGC 1 cut(s) 281
Cac8I GCNNGC 3 cut(s) 407, 1827, 2201
CfoI GCGC 4 cut(s) 78, 1525, 1836, 2167
Cfr10I RCCGGY 1 cut(s) 2514
Cfr13I GGNCC 8 cut(s) 43, 102, 204, 282, 723, 865, 1121, 2202
ClaI ATCGAT 1 cut(s) 937
CseI GACGC 1 cut(s) 72
Csp6I GTAC 9 cut(s) 188, 589, 907, 1159, 1322, 1612, 1947, 2121, 2314
CviAII CATG 8 cut(s) 533, 869, 1063, 1270, 1653, 1732, 2058, 2423
CviQI GTAC 9 cut(s) 188, 589, 907, 1159, 1322, 1612, 1947, 2121, 2314
DpnI GATC 8 cut(s) 138, 527, 819, 936, 969, 1725, 1789, 2409
DpnII GATC 8 cut(s) 136, 525, 817, 934, 967, 1723, 1787, 2407
DriI GACNNNNNGTC 1 cut(s) 1432
EaeI YGGCCR 1 cut(s) 314
Eam1104I CTCTTC 4 cut(s) 682, 1665, 2368, 2388
Eam1105I GACNNNNNGTC 1 cut(s) 1432
EarI CTCTTC 4 cut(s) 682, 1665, 2368, 2388
Ecl136II GAGCTC 1 cut(s) 57
Eco130I CCWWGG 2 cut(s) 285, 984
Eco24I GRGCYC 1 cut(s) 59
Eco31I GGTCTC 1 cut(s) 120
Eco32I GATATC 1 cut(s) 1449
Eco47I GGWCC 5 cut(s) 102, 204, 723, 865, 1121
Eco53kI GAGCTC 1 cut(s) 57
Eco88I CYCGRG 2 cut(s) 563, 886
EcoICRI GAGCTC 1 cut(s) 57
EcoRII CCWGG 3 cut(s) 1249, 1442, 1457
EcoRV GATATC 1 cut(s) 1449
EcoT14I CCWWGG 2 cut(s) 285, 984
EcoT38I GRGCYC 1 cut(s) 59
ErhI CCWWGG 2 cut(s) 285, 984
FaeI CATG 8 cut(s) 536, 872, 1066, 1273, 1656, 1735, 2061, 2426
FaqI GGGAC 4 cut(s) 190, 518, 546, 1232
FatI CATG 8 cut(s) 532, 868, 1062, 1269, 1652, 1731, 2057, 2422
FauI CCCGC 2 cut(s) 398, 515
FauNDI CATATG 1 cut(s) 931
FbaI TGATCA 1 cut(s) 1723
Fnu4HI GCNGC 5 cut(s) 801, 1381, 1647, 2197, 2256
FokI GGATG 6 cut(s) 29, 916, 1085, 1181, 1321, 2012
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 5 cut(s) 801, 1381, 1647, 2197, 2256
FspBI CTAG 7 cut(s) 131, 225, 993, 1371, 1745, 2243, 2363
GlaI GCGC 4 cut(s) 77, 1524, 1835, 2166
GluI GCNGC 5 cut(s) 801, 1381, 1647, 2197, 2256
GsaI CCCAGC 2 cut(s) 49, 211
GsuI CTGGAG 2 cut(s) 1405, 1441
HaeII RGCGCY 2 cut(s) 79, 1526
HaeIII GGCC 6 cut(s) 37, 44, 283, 316, 1058, 2203
HapII CCGG 3 cut(s) 41, 100, 2515
HgaI GACGC 1 cut(s) 72
HhaI GCGC 4 cut(s) 78, 1525, 1836, 2167
Hin1II CATG 8 cut(s) 536, 872, 1066, 1273, 1656, 1735, 2061, 2426
Hin6I GCGC 4 cut(s) 76, 1523, 1834, 2165
HinP1I GCGC 4 cut(s) 76, 1523, 1834, 2165
HindIII AAGCTT 1 cut(s) 362
HpaII CCGG 3 cut(s) 41, 100, 2515
HphI GGTGA 9 cut(s) 16, 320, 520, 748, 1265, 1503, 1548, 1563, 2265
Hpy166II GTNNAC 4 cut(s) 589, 726, 893, 1772
Hpy188III TCNNGA 8 cut(s) 110, 392, 563, 993, 1177, 1297, 1785, 2501
Hpy8I GTNNAC 4 cut(s) 589, 726, 893, 1772
Hpy99I CGWCG 2 cut(s) 88, 1510
HpyCH4III ACNGT 5 cut(s) 161, 852, 1928, 1946, 2342
HpyCH4IV ACGT 3 cut(s) 669, 1221, 1965
HpyF10VI GCNNNNNNNGC 4 cut(s) 73, 244, 1082, 1831
HpySE526I ACGT 3 cut(s) 669, 1221, 1965
Hsp92II CATG 8 cut(s) 536, 872, 1066, 1273, 1656, 1735, 2061, 2426
HspAI GCGC 4 cut(s) 76, 1523, 1834, 2165
Ksp22I TGATCA 1 cut(s) 1723
Kzo9I GATC 8 cut(s) 136, 525, 817, 934, 967, 1723, 1787, 2407
Lsp1109I GCAGC 4 cut(s) 812, 1392, 1658, 2242
LweI GCATC 7 cut(s) 7, 475, 769, 952, 1063, 1159, 1343
MaeI CTAG 7 cut(s) 131, 225, 993, 1371, 1745, 2243, 2363
MaeII ACGT 3 cut(s) 669, 1221, 1965
MaeIII GTNAC 6 cut(s) 846, 1509, 1554, 1698, 1961, 2399
MalI GATC 8 cut(s) 138, 527, 819, 936, 969, 1725, 1789, 2409
MbiI CCGCTC 1 cut(s) 522
MboI GATC 8 cut(s) 136, 525, 817, 934, 967, 1723, 1787, 2407
MfeI CAATTG 1 cut(s) 1329
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 2 cut(s) 59, 1393
MlsI TGGCCA 1 cut(s) 316
MluNI TGGCCA 1 cut(s) 316
MlyI GAGTC 4 cut(s) 1238, 1900, 2000, 2048
MmeI TCCRAC 2 cut(s) 641, 2206
Mox20I TGGCCA 1 cut(s) 316
MroXI GAANNNNTTC 1 cut(s) 68
MscI TGGCCA 1 cut(s) 316
MseI TTAA 6 cut(s) 194, 513, 1239, 1854, 2463, 2472
Msp20I TGGCCA 1 cut(s) 316
MspA1I CMGCKG 2 cut(s) 1383, 2199
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 3 cut(s) 41, 100, 2515
MspR9I CCNGG 4 cut(s) 100, 1251, 1444, 1459
MunI CAATTG 1 cut(s) 1329
MvaI CCWGG 3 cut(s) 1251, 1444, 1459
MwoI GCNNNNNNNGC 4 cut(s) 73, 244, 1082, 1831
NciI CCSGG 1 cut(s) 100
NdeI CATATG 1 cut(s) 931
NdeII GATC 8 cut(s) 136, 525, 817, 934, 967, 1723, 1787, 2407
NlaIII CATG 8 cut(s) 536, 872, 1066, 1273, 1656, 1735, 2061, 2426
NlaIV GGNNCC 3 cut(s) 206, 866, 2105
NmeAIII GCCGAG 1 cut(s) 241
NmuCI GTSAC 5 cut(s) 1509, 1554, 1698, 1961, 2399
NspI RCATGY 1 cut(s) 1273
PciI ACATGT 1 cut(s) 1269
PdmI GAANNNNTTC 1 cut(s) 68
PfoI TCCNGGA 1 cut(s) 98
PkrI GCNGC 5 cut(s) 802, 1382, 1648, 2198, 2257
PleI GAGTC 4 cut(s) 1237, 1899, 1999, 2048
PpsI GAGTC 4 cut(s) 1237, 1899, 1999, 2048
Ppu21I YACGTR 1 cut(s) 1966
PscI ACATGT 1 cut(s) 1269
Psp124BI GAGCTC 1 cut(s) 59
Psp6I CCWGG 3 cut(s) 1249, 1442, 1457
PspFI CCCAGC 2 cut(s) 45, 207
PspGI CCWGG 3 cut(s) 1249, 1442, 1457
PspN4I GGNNCC 3 cut(s) 206, 866, 2105
PspPI GGNCC 8 cut(s) 43, 102, 204, 282, 723, 865, 1121, 2202
PstI CTGCAG 1 cut(s) 1648
PsuI RGATCY 1 cut(s) 136
PvuII CAGCTG 1 cut(s) 1383
RsaI GTAC 9 cut(s) 189, 590, 908, 1160, 1323, 1613, 1948, 2122, 2315
RsaNI GTAC 9 cut(s) 188, 589, 907, 1159, 1322, 1612, 1947, 2121, 2314
SacI GAGCTC 1 cut(s) 59
SaqAI TTAA 6 cut(s) 194, 513, 1239, 1854, 2463, 2472
SatI GCNGC 5 cut(s) 801, 1381, 1647, 2197, 2256
Sau3AI GATC 8 cut(s) 136, 525, 817, 934, 967, 1723, 1787, 2407
Sau96I GGNCC 8 cut(s) 43, 102, 204, 282, 723, 865, 1121, 2202
ScaI AGTACT 2 cut(s) 1613, 1948
SchI GAGTC 4 cut(s) 1238, 1900, 2000, 2048
ScrFI CCNGG 4 cut(s) 100, 1251, 1444, 1459
SduI GDGCHC 2 cut(s) 59, 1393
SfaNI GCATC 7 cut(s) 7, 475, 769, 952, 1063, 1159, 1343
SfcI CTRYAG 1 cut(s) 1644
SinI GGWCC 5 cut(s) 102, 204, 723, 865, 1121
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
SsiI CCGC 4 cut(s) 24, 405, 522, 2197
SspI AATATT 3 cut(s) 1279, 1975, 2508
SspMI CTAG 7 cut(s) 131, 225, 993, 1371, 1745, 2243, 2363
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 4 cut(s) 98, 1249, 1442, 1457
StyI CCWWGG 2 cut(s) 285, 984
TaaI ACNGT 5 cut(s) 161, 852, 1928, 1946, 2342
TaiI ACGT 3 cut(s) 672, 1224, 1968
TaqI TCGA 6 cut(s) 53, 937, 1178, 1955, 1994, 2337
TaqII GACCGA 2 cut(s) 192, 711
TatI WGTACW 4 cut(s) 588, 906, 1611, 1946
TauI GCSGC 1 cut(s) 2199
Tru1I TTAA 6 cut(s) 194, 513, 1239, 1854, 2463, 2472
Tru9I TTAA 6 cut(s) 194, 513, 1239, 1854, 2463, 2472
TscAI CASTG 7 cut(s) 166, 352, 857, 1933, 2098, 2347, 2496
TseFI GTSAC 5 cut(s) 1509, 1554, 1698, 1961, 2399
TseI GCWGC 4 cut(s) 800, 1380, 1646, 2255
Tsp45I GTSAC 5 cut(s) 1509, 1554, 1698, 1961, 2399
TspRI CASTG 7 cut(s) 166, 352, 857, 1933, 2098, 2347, 2496
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 5 cut(s) 102, 204, 723, 865, 1121
XapI RAATTY 4 cut(s) 1290, 1501, 1812, 1904
XbaI TCTAGA 1 cut(s) 992
XceI RCATGY 1 cut(s) 1273
XcmI CCANNNNNNNNNTGG 1 cut(s) 1161
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 7 cut(s) 131, 225, 993, 1371, 1745, 2243, 2363
ZrmI AGTACT 2 cut(s) 1613, 1948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.