MD07G1280200.v1.1

Tic22-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012179
Physical Location & Seq
Forward (+)
34418766 .. 34422108
3343 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1280200.v1.1.491

Sequence Viewer

Length: 1638 bp
ATGGCCTCACCATCCGAACCACCCCACACCAGGCAGCAACTCAGACCCTCCATAACCCACCTGGTCCACTCCACCACATCCAATCTCCTCTCGCTCCTCGCCTCCCCCAAAACGACGCCGTCGCCATCGCTTTCCACGACTCCTCCGGAAATCCGCGTCGCGCTCTTCCTCCCCAACTTGCCAAAACCACTCGCTTTCCAGCCCGACTCCAAACTCTCCGCCATGAAGGGCGTGGCGTCATCGCCCGAGTCGACCTCCGGGTTCCCGTCAACGGTCAGGATTGCAGGGCTGAACTCGAATATCAAGGGCAGCGGTGGGCCGGCGTTTGTGGGCCAGGTCTTCAGTATGTGCGACCTCTCCGGGACTGGTCTGATGGCCGTCTCCACCCACTTCGACATCCCCTTTATTTCCAAAAGAACTCCCGAGTGGCTGAAGAAGATGTTTAAATCAATTACTAACAGTGAGAGAAACGGCCCCGTGTTTCGATTTTTCATGGATTTAGGCGATGCAGTTACTTATGTGAAACGGCTGAATATTCCGAGCGGCGTGGTGGGTGCTTGTCGTCTCGATTTAGCATATGAACATTTCAAGGAAAAGCCCCACTTATTCCAGTTTGTTCCGAATGAAAAACAGGTCAAGGCAGCTAACAAACTTCTCAAGACAATGCCGCAGAGTGGCAGATGCAAAAGGGTTGATGGAGTTCCAGTTTTTAGTGCTCAAAACTTGGATATTGCAATAGCAAGCTCAGATGGGATCAAATGGTATACTCCCTACTTTTTTGATAAAAACATGCTTGATAACATTCTCGAGGAATCTGTAGATCAGCATTTCCATTCTTTAATTCAAACTCGGCACACGCAGCGGCGACATGATGTGATTGATGACAACATACCAGCAGAAGTAATTGAAGAGATGGGCGATAACTTGTGGGAGCCTCCAGAGGTTCAGGAAGTGATGGATGAGATAGGGAATTCTGGAATACCCTTGAGTGTCATTTCTAAAGCCGCTGAAATGCAGCTCCTTTATGCTGTTGATAAAGTACTTCTGGGTAATAGGTGGTTACGGAAAGCAACAGGCATTCAACCCAAATTCCCTTATATGGTTGACTCTTTCGAGAGAAGGAGTGCAGCCTCTTTTCTTAGAGCTTCTCAGTCAGCCAGATGCCTTACGAATGGGGAAGCAGTAAATGATAACGAAGATTCTCTAGATTGTAGCACTTCGAAGATTAAATCAAAGGATGATATTCACACCAATAAAGGAAACAAAACAGATCTACGGTTTCCTTTCAGTGATTGGTTTAGTCATCTGTGGTCGAAACAGAAGGACCAAAATGAAGAGTTGTCAAAGGAACGCATAGAGCAGAGCACACTGCAAAATCCAAACCTTCCTAAGATTACTATGGTTGGCATTTCAACTGGTGCGGCAGGGCCGATGAGTAAAGCAAATTTAAAGAAGACGATGGAGGATTTAACAAAAGAATTGGAGCAGACGGATTCGGGAAATGCTTTTGGTAGCGATACAGGTAATGAGTTGAGATTTGAAGATAGAGATCCACTGTTTGTGGCAAACGTAGGTGATTATTATTCTGGCATGGCAAAGACGGGTTCGGCTAAATGGGTTCGTGGAGGAAACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tic22

Protein Analysis

546

Amino Acids

60.39

Weight (kDa)

7.2

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tic22 PF04278 196 - 272 7e-07 Tic22-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62650
fragaria_vesca FvH4_7g30450 FvH4_7g30450
malus_domestica MD01G1210100.v1.1 MD07G1280200.v1.1
prunus_persica Prupe.2G302400_v2.0.a1 Prupe.2G302400_v2.0.a1 Prupe.2G302400_v2.0.a1
pyrus_communis pycom07g25480
rosa_chinensis RchiOBHm_Chr1g0378611
rosa_laevigata RLG00000026419
rosa_multiflora Rmu_sc0002395.1_g000010
rosa_roxburghii Rroxscaffold_4G00280360
rosa_rugosa Rorug01G0409900
rosa_samantha Rh1AG431400 Rh1BG389700 Rh1CG403000 Rh1DG420900
rosa_wichuraiana Rw1G037790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 543
AccI GTMKAC 2 cut(s) 251, 764
AccII CGCG 2 cut(s) 156, 161
AccIII TCCGGA 1 cut(s) 145
AciI CCGC 8 cut(s) 154, 219, 312, 543, 668, 862, 1005, 1421
AclWI GGATC 2 cut(s) 761, 1544
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 3 cut(s) 970, 1088, 1444
AcuI CTGAAG 2 cut(s) 325, 452
AcyI GRCGYC 2 cut(s) 116, 236
AfaI GTAC 1 cut(s) 1041
AfiI CCNNNNNNNGG 4 cut(s) 30, 271, 674, 1099
AgsI TTSAA 6 cut(s) 589, 845, 908, 1082, 1413, 1541
AjnI CCWGG 3 cut(s) 29, 60, 333
AluBI AGCT 4 cut(s) 644, 744, 1018, 1145
AluI AGCT 4 cut(s) 644, 744, 1018, 1145
Alw21I GWGCWC 2 cut(s) 718, 1367
Alw26I GTCTC 2 cut(s) 385, 569
AlwI GGATC 2 cut(s) 761, 1544
Ama87I CYCGRG 3 cut(s) 245, 422, 806
Aor13HI TCCGGA 1 cut(s) 145
AoxI GGCC 6 cut(s) 3, 317, 331, 375, 472, 1427
ApeKI GCWGC 6 cut(s) 34, 309, 641, 859, 1015, 1127
ApoI RAATTY 3 cut(s) 970, 1088, 1444
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 6 cut(s) 64, 317, 331, 473, 1324, 1427
AsuC2I CCSGG 2 cut(s) 259, 361
AsuHPI GGTGA 1 cut(s) 1586
AsuII TTCGAA 1 cut(s) 1220
AvaI CYCGRG 3 cut(s) 245, 422, 806
AvaII GGWCC 2 cut(s) 64, 1324
BbsI GAAGAC 2 cut(s) 331, 1460
Bbv12I GWGCWC 2 cut(s) 718, 1367
BbvI GCAGC 6 cut(s) 46, 321, 653, 871, 1027, 1139
BccI CCATC 8 cut(s) 19, 133, 367, 689, 743, 907, 949, 1453
BceAI ACGGC 4 cut(s) 103, 362, 487, 542
BciT130I CCWGG 3 cut(s) 31, 62, 335
BcnI CCSGG 2 cut(s) 259, 361
BcoDI GTCTC 2 cut(s) 385, 569
BfaI CTAG 1 cut(s) 1205
BfmI CTRYAG 1 cut(s) 816
BglII AGATCT 1 cut(s) 1270
BmcAI AGTACT 1 cut(s) 1041
Bme1390I CCNGG 5 cut(s) 31, 62, 259, 335, 361
Bme18I GGWCC 2 cut(s) 64, 1324
BmeT110I CYCGRG 3 cut(s) 245, 422, 806
BmgT120I GGNCC 6 cut(s) 64, 317, 331, 473, 1324, 1427
BmiI GGNNCC 3 cut(s) 263, 475, 933
BmrFI CCNGG 5 cut(s) 31, 62, 259, 335, 361
BmsI GCATC 3 cut(s) 496, 671, 1151
BpiI GAAGAC 2 cut(s) 331, 1460
BplI GAGNNNNNCTC 2 cut(s) 239, 271
BpmI CTGGAG 1 cut(s) 921
Bpu14I TTCGAA 1 cut(s) 1220
BpuEI CTTGAG 2 cut(s) 641, 1006
BpuMI CCSGG 2 cut(s) 259, 361
BsaBI GATNNNNATC 1 cut(s) 1548
BsaHI GRCGYC 2 cut(s) 116, 236
BsaWI WCCGGW 1 cut(s) 145
BsaXI ACNNNNNCTCC 4 cut(s) 127, 157, 690, 720
Bsc4I CCNNNNNNNGG 4 cut(s) 30, 271, 674, 1099
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 4 cut(s) 370, 610, 704, 1420
Bse8I GATNNNNATC 1 cut(s) 1548
BseAI TCCGGA 1 cut(s) 145
BseBI CCWGG 3 cut(s) 31, 62, 335
BseGI GGATG 5 cut(s) 11, 77, 396, 964, 1243
BseJI GATNNNNATC 1 cut(s) 1548
BseLI CCNNNNNNNGG 4 cut(s) 30, 271, 674, 1099
BseMII CTCAG 3 cut(s) 55, 759, 1163
BseNI ACTGG 4 cut(s) 370, 610, 704, 1420
BseRI GAGGAG 3 cut(s) 77, 86, 132
BseXI GCAGC 6 cut(s) 46, 321, 653, 871, 1027, 1139
BsgI GTGCAG 1 cut(s) 1146
Bsh1236I CGCG 2 cut(s) 156, 161
BshFI GGCC 6 cut(s) 5, 319, 333, 377, 474, 1429
BsiHKAI GWGCWC 2 cut(s) 718, 1367
BsiHKCI CYCGRG 3 cut(s) 245, 422, 806
BsiSI CCGG 4 cut(s) 146, 258, 320, 360
BslFI GGGAC 1 cut(s) 376
BslI CCNNNNNNNGG 4 cut(s) 30, 271, 674, 1099
BsmAI GTCTC 2 cut(s) 385, 569
BsmBI CGTCTC 2 cut(s) 385, 569
BsmFI GGGAC 1 cut(s) 376
BsmI GAATGC 1 cut(s) 1077
BsnI GGCC 6 cut(s) 5, 319, 333, 377, 474, 1429
BsoBI CYCGRG 3 cut(s) 245, 422, 806
Bsp119I TTCGAA 1 cut(s) 1220
Bsp1286I GDGCHC 2 cut(s) 718, 1367
Bsp13I TCCGGA 1 cut(s) 145
Bsp143I GATC 4 cut(s) 753, 820, 1270, 1549
BspACI CCGC 8 cut(s) 154, 219, 312, 543, 668, 862, 1005, 1421
BspANI GGCC 6 cut(s) 5, 319, 333, 377, 474, 1429
BspCNI CTCAG 3 cut(s) 54, 758, 1162
BspEI TCCGGA 1 cut(s) 145
BspFNI CGCG 2 cut(s) 156, 161
BspLI GGNNCC 3 cut(s) 263, 475, 933
BspPI GGATC 2 cut(s) 761, 1544
BspQI GCTCTTC 1 cut(s) 170
BspT104I TTCGAA 1 cut(s) 1220
BsrBI CCGCTC 1 cut(s) 543
BsrFI RCCGGY 1 cut(s) 319
BsrI ACTGG 4 cut(s) 370, 610, 704, 1420
BssAI RCCGGY 1 cut(s) 319
BssMI GATC 4 cut(s) 753, 820, 1270, 1549
BssNAI GTATAC 1 cut(s) 765
BssNI GRCGYC 2 cut(s) 116, 236
Bst1107I GTATAC 1 cut(s) 765
Bst2UI CCWGG 3 cut(s) 31, 62, 335
Bst4CI ACNGT 4 cut(s) 274, 461, 1278, 1557
Bst6I CTCTTC 3 cut(s) 170, 903, 1329
BstACI GRCGYC 2 cut(s) 116, 236
BstBI TTCGAA 1 cut(s) 1220
BstC8I GCNNGC 2 cut(s) 321, 742
BstDEI CTNAG 5 cut(s) 41, 745, 1139, 1149, 1389
BstF5I GGATG 5 cut(s) 11, 77, 396, 964, 1243
BstFNI CGCG 2 cut(s) 156, 161
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 4 cut(s) 756, 823, 1273, 1552
BstMAI GTCTC 2 cut(s) 385, 569
BstMBI GATC 4 cut(s) 753, 820, 1270, 1549
BstMWI GCNNNNNNNGC 1 cut(s) 859
BstNI CCWGG 3 cut(s) 31, 62, 335
BstNSI RCATGY 1 cut(s) 793
BstSCI CCNGG 5 cut(s) 29, 60, 257, 333, 359
BstSFI CTRYAG 1 cut(s) 816
BstUI CGCG 2 cut(s) 156, 161
BstV1I GCAGC 6 cut(s) 46, 321, 653, 871, 1027, 1139
BstV2I GAAGAC 2 cut(s) 331, 1460
BstX2I RGATCY 2 cut(s) 1270, 1549
BstYI RGATCY 2 cut(s) 1270, 1549
BstZ17I GTATAC 1 cut(s) 765
BsuRI GGCC 6 cut(s) 5, 319, 333, 377, 474, 1429
BtgZI GCGATG 3 cut(s) 111, 225, 519
BtsCI GGATG 5 cut(s) 11, 77, 396, 964, 1243
BtsI GCAGTG 1 cut(s) 1367
BtsIMutI CAGTG 4 cut(s) 466, 1294, 1367, 1553
Cac8I GCNNGC 2 cut(s) 321, 742
CfoI GCGC 1 cut(s) 163
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 6 cut(s) 64, 317, 331, 473, 1324, 1427
CseI GACGC 3 cut(s) 124, 145, 225
CsiI ACCWGGT 1 cut(s) 60
Csp6I GTAC 1 cut(s) 1040
CviAII CATG 5 cut(s) 223, 493, 790, 869, 1591
CviQI GTAC 1 cut(s) 1040
DdeI CTNAG 5 cut(s) 41, 745, 1139, 1149, 1389
DpnI GATC 4 cut(s) 755, 822, 1272, 1551
DpnII GATC 4 cut(s) 753, 820, 1270, 1549
DraI TTTAAA 2 cut(s) 445, 1449
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 3 cut(s) 170, 903, 1329
EarI CTCTTC 3 cut(s) 170, 903, 1329
EciI GGCGGA 1 cut(s) 208
Eco47I GGWCC 2 cut(s) 64, 1324
Eco57I CTGAAG 2 cut(s) 325, 452
Eco88I CYCGRG 3 cut(s) 245, 422, 806
EcoRI GAATTC 1 cut(s) 970
EcoRII CCWGG 3 cut(s) 29, 60, 333
Esp3I CGTCTC 2 cut(s) 385, 569
FaeI CATG 5 cut(s) 226, 496, 793, 872, 1594
FalI AAGNNNNNCTT 2 cut(s) 587, 619
FaqI GGGAC 1 cut(s) 376
FatI CATG 5 cut(s) 222, 492, 789, 868, 1590
FauNDI CATATG 1 cut(s) 577
FblI GTMKAC 2 cut(s) 251, 764
FokI GGATG 4 cut(s) 64, 383, 971, 1250
FspBI CTAG 1 cut(s) 1205
GlaI GCGC 1 cut(s) 162
GsuI CTGGAG 1 cut(s) 921
HaeIII GGCC 6 cut(s) 5, 319, 333, 377, 474, 1429
HapII CCGG 4 cut(s) 146, 258, 320, 360
HgaI GACGC 3 cut(s) 124, 145, 225
HhaI GCGC 1 cut(s) 163
Hin1I GRCGYC 2 cut(s) 116, 236
Hin1II CATG 5 cut(s) 226, 496, 793, 872, 1594
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HincII GTYRAC 3 cut(s) 252, 270, 1105
HindII GTYRAC 3 cut(s) 252, 270, 1105
HinfI GANTC 7 cut(s) 139, 206, 248, 812, 1106, 1199, 1493
HpaII CCGG 4 cut(s) 146, 258, 320, 360
HphI GGTGA 1 cut(s) 1586
Hpy166II GTNNAC 5 cut(s) 67, 252, 270, 765, 1105
Hpy188I TCNGA 6 cut(s) 16, 44, 372, 540, 621, 748
Hpy8I GTNNAC 5 cut(s) 67, 252, 270, 765, 1105
Hpy99I CGWCG 3 cut(s) 118, 124, 161
HpyAV CCTTC 4 cut(s) 220, 1113, 1315, 1394
HpyCH4III ACNGT 4 cut(s) 274, 461, 1278, 1557
HpyCH4IV ACGT 1 cut(s) 1569
HpyCH4V TGCA 7 cut(s) 284, 509, 684, 734, 1015, 1127, 1372
HpyF10VI GCNNNNNNNGC 1 cut(s) 859
HpyF3I CTNAG 5 cut(s) 41, 745, 1139, 1149, 1389
HpySE526I ACGT 1 cut(s) 1569
Hsp92I GRCGYC 2 cut(s) 116, 236
Hsp92II CATG 5 cut(s) 226, 496, 793, 872, 1594
HspAI GCGC 1 cut(s) 161
Kpn2I TCCGGA 1 cut(s) 145
KroI GCCGGC 1 cut(s) 319
KroNI GCCGGC 1 cut(s) 321
Kzo9I GATC 4 cut(s) 753, 820, 1270, 1549
LguI GCTCTTC 1 cut(s) 170
LmnI GCTCC 4 cut(s) 99, 931, 1023, 1483
Lsp1109I GCAGC 6 cut(s) 46, 321, 653, 871, 1027, 1139
LweI GCATC 3 cut(s) 496, 671, 1151
MabI ACCWGGT 1 cut(s) 60
MaeI CTAG 1 cut(s) 1205
MaeII ACGT 1 cut(s) 1569
MaeIII GTNAC 2 cut(s) 511, 1059
MalI GATC 4 cut(s) 755, 822, 1272, 1551
MbiI CCGCTC 1 cut(s) 543
MboI GATC 4 cut(s) 753, 820, 1270, 1549
MflI RGATCY 2 cut(s) 1270, 1549
MhlI GDGCHC 2 cut(s) 718, 1367
MluCI AATT 8 cut(s) 450, 840, 903, 970, 1088, 1444, 1478, 1633
MlyI GAGTC 4 cut(s) 133, 200, 257, 1100
MroI TCCGGA 1 cut(s) 145
MroNI GCCGGC 1 cut(s) 319
MseI TTAA 5 cut(s) 444, 839, 1227, 1448, 1469
MspA1I CMGCKG 3 cut(s) 312, 862, 1007
MspI CCGG 4 cut(s) 146, 258, 320, 360
MspR9I CCNGG 5 cut(s) 31, 62, 259, 335, 361
Mva1269I GAATGC 1 cut(s) 1077
MvaI CCWGG 3 cut(s) 31, 62, 335
MvnI CGCG 2 cut(s) 156, 161
MwoI GCNNNNNNNGC 1 cut(s) 859
NaeI GCCGGC 1 cut(s) 321
NciI CCSGG 2 cut(s) 259, 361
NdeI CATATG 1 cut(s) 577
NdeII GATC 4 cut(s) 753, 820, 1270, 1549
NgoMIV GCCGGC 1 cut(s) 319
NlaIII CATG 5 cut(s) 226, 496, 793, 872, 1594
NlaIV GGNNCC 3 cut(s) 263, 475, 933
NmeAIII GCCGAG 1 cut(s) 829
NspI RCATGY 1 cut(s) 793
NspV TTCGAA 1 cut(s) 1220
PaeR7I CTCGAG 1 cut(s) 806
PciSI GCTCTTC 1 cut(s) 170
PcsI WCGNNNNNNNCGW 2 cut(s) 134, 248
PctI GAATGC 1 cut(s) 1077
PdiI GCCGGC 1 cut(s) 321
PfeI GAWTC 3 cut(s) 812, 1199, 1493
PflFI GACNNNGTC 1 cut(s) 118
PfoI TCCNGGA 1 cut(s) 359
PleI GAGTC 4 cut(s) 133, 200, 256, 1100
PpsI GAGTC 4 cut(s) 133, 200, 256, 1100
Psp6I CCWGG 3 cut(s) 29, 60, 333
PspGI CCWGG 3 cut(s) 29, 60, 333
PspN4I GGNNCC 3 cut(s) 263, 475, 933
PspPI GGNCC 6 cut(s) 64, 317, 331, 473, 1324, 1427
PsuI RGATCY 2 cut(s) 1270, 1549
PsyI GACNNNGTC 1 cut(s) 118
RsaI GTAC 1 cut(s) 1041
RsaNI GTAC 1 cut(s) 1040
SalI GTCGAC 1 cut(s) 250
SapI GCTCTTC 1 cut(s) 170
SaqAI TTAA 5 cut(s) 444, 839, 1227, 1448, 1469
Sau3AI GATC 4 cut(s) 753, 820, 1270, 1549
Sau96I GGNCC 6 cut(s) 64, 317, 331, 473, 1324, 1427
ScaI AGTACT 1 cut(s) 1041
SchI GAGTC 4 cut(s) 133, 200, 257, 1100
ScrFI CCNGG 5 cut(s) 31, 62, 259, 335, 361
SduI GDGCHC 2 cut(s) 718, 1367
SexAI ACCWGGT 1 cut(s) 60
SfaNI GCATC 3 cut(s) 496, 671, 1151
SfcI CTRYAG 1 cut(s) 816
Sfr274I CTCGAG 1 cut(s) 806
SfuI TTCGAA 1 cut(s) 1220
SinI GGWCC 2 cut(s) 64, 1324
SlaI CTCGAG 1 cut(s) 806
SmlI CTYRAG 3 cut(s) 656, 806, 985
SmoI CTYRAG 3 cut(s) 656, 806, 985
Sse9I AATT 8 cut(s) 450, 840, 903, 970, 1088, 1444, 1478, 1633
SsiI CCGC 8 cut(s) 154, 219, 312, 543, 668, 862, 1005, 1421
SspI AATATT 1 cut(s) 535
SspMI CTAG 1 cut(s) 1205
StyD4I CCNGG 5 cut(s) 29, 60, 257, 333, 359
TaaI ACNGT 4 cut(s) 274, 461, 1278, 1557
TaiI ACGT 1 cut(s) 1572
TaqI TCGA 9 cut(s) 251, 296, 393, 484, 567, 807, 1113, 1220, 1313
TasI AATT 8 cut(s) 450, 840, 903, 970, 1088, 1444, 1478, 1633
TatI WGTACW 1 cut(s) 1039
TauI GCSGC 5 cut(s) 546, 670, 865, 1007, 1424
TfiI GAWTC 3 cut(s) 812, 1199, 1493
Tru1I TTAA 5 cut(s) 444, 839, 1227, 1448, 1469
Tru9I TTAA 5 cut(s) 444, 839, 1227, 1448, 1469
TscAI CASTG 4 cut(s) 466, 1294, 1374, 1560
TseI GCWGC 6 cut(s) 34, 309, 641, 859, 1015, 1127
TspDTI ATGAA 5 cut(s) 239, 481, 594, 639, 1347
TspGWI ACGGA 2 cut(s) 1078, 1505
TspRI CASTG 4 cut(s) 466, 1294, 1374, 1560
Tth111I GACNNNGTC 1 cut(s) 118
VpaK11BI GGWCC 2 cut(s) 64, 1324
XapI RAATTY 3 cut(s) 970, 1088, 1444
XbaI TCTAGA 1 cut(s) 1204
XceI RCATGY 1 cut(s) 793
XcmI CCANNNNNNNNNTGG 2 cut(s) 58, 229
XhoI CTCGAG 1 cut(s) 806
XmiI GTMKAC 2 cut(s) 251, 764
XspI CTAG 1 cut(s) 1205
ZrmI AGTACT 1 cut(s) 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.