Rorug01G0409900

Tic22-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0012179
Physical Location & Seq
Reverse (-)
51556608 .. 51558544
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0409900.1

Sequence Viewer

Length: 1581 bp
ATGGGGACATCAGATTTGAAGAAGTCGGATTCATGTGAGCAGGACAACAATGTCAGTCAAGATGGTTCTATATGGCTGGTAGAAAATCTTGAAGAGATGGACCAGAGAATTAAGCAAATGCTTAAGCTGATAAAAGAAGATGGTGATTCTTTGCCTAGAAATGTTGCAGAAGATTCTTATAAGAAGCCAGAGTTAATTGAGGAATTTCAGCGATTGTATCGATCACTGGCTGGGTGCTATGGTCACTTGGCAAAAGAAGCACATATGCAGACTCCTGATGGAAAGGTGGGTTTGCATAAATCTGGCCATCAGGTCATTGGTTTAGATATGTCACCAAGCTCAGGTGGTTCTAGTCCTGCTCTTTCTTTAAAGAAAGGCACCGAATCATCTTCCTCATCATCATTGGAGTCTGAGTCAGAATCTCTTAACTTATCTCCCAGCAATTACTCGATTCCACCTCTGAACATGGACTTTGACTCGCAGGGATGGGAGAGGATTATTGAACTAGAGGATGAACTTTCCAGCATGAAAGAGAAGCTCCAGACAAGGGAGGCTGATCTCGAACAGGAGAAATTACGGGTATCAGATCTGCAAAAACAGATAGCTGAGTTGGAGAGTCGTGCATCAGAAACAGATAATGACATTGGTAGATTGGTGAGAGAGTTGGAAGTAATTACAGCAAGGCTCAAGGGGTCGGATGAGGAGAATGCAAGGTTGAAGCAGGAGGTTACTGAGAAAGTGTCTGAAGGTGTTAAAGAAATGCAAGGTCATCTTGCAGTGGCTGAGGAAGATATAGCCATGTTGGAAGCTCAACTTGACTCGGAGAGGAAGCATGTTTTGGAGCTGCAGGAGAGGATTGTGAAGTACGATGCCGATTTGGTTGGCCGTGATCTTGAGTTGATGGAATTGAAGAGTGCTTTGCATGATGCACAGGACCAGTTTTCTCTGGAGAGAGCAGACATGCAGTTTCATATTTCTGGTTTGTCAGAGAATCAAACCATCTTGGAGACCAGGCTTGAAGAATGGGAGTGGAAGAACAGGAATCTAGAAGATGAAATAAGGCAACACCAAACTGATAAGATGGAGCTGGAAAAGATGCGTGTTGCACAAGAGATGGTTTTGCAAGGCGAAATAAGTTGGCTGAAGGTAGAACTCGCTGAACGAGGTAAACATGTGGAAGCTGTGAATAAGGACTTTGACAAATTCAAACTGAAATATGATATGCTAATGGCAGAGAAGGATGAGCTCAATGCTAAGGTTCACACGCTTATGGCGAATGTGAGTTGCCGTGATGATCAAATTAGGGAAATGGAAGGACATCTATGCCGGTTACATACAGACCATGAGGATCTGATTGCTGGGTCTGAAAGTGCACGCAAGTTAGTAGATGAACTGAAATTGAGAGTGGAGGAGTTGCAGGAAGAGGTGAATAGGCAGAGAGTTGTGATATCAGACGGGGCTGAGGAGAAAAGAGAGGCTATACGACAGCTATGTTTCTCACTGGAGCATTATAGGAGTGGATATAAAGAACTTCACCAAGCATTTAAGGGGCACAAGTGGCGAGCAGTTGCTGCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tic22

Protein Analysis

526

Amino Acids

60.24

Weight (kDa)

4.93

Isoelectric Point (pI)

46.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 15 - 80 1.1e-14 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62650
fragaria_vesca FvH4_7g30450 FvH4_7g30450
malus_domestica MD01G1210100.v1.1 MD07G1280200.v1.1
prunus_persica Prupe.2G302400_v2.0.a1 Prupe.2G302400_v2.0.a1 Prupe.2G302400_v2.0.a1
pyrus_communis pycom07g25480
rosa_chinensis RchiOBHm_Chr1g0378611
rosa_laevigata RLG00000026419
rosa_multiflora Rmu_sc0002395.1_g000010
rosa_roxburghii Rroxscaffold_4G00280360
rosa_rugosa Rorug01G0409900
rosa_samantha Rh1AG431400 Rh1BG389700 Rh1CG403000 Rh1DG420900
rosa_wichuraiana Rw1G037790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 180
AasI GACNNNNNNGTC 1 cut(s) 50
AccB1I GGYRCC 1 cut(s) 377
AclWI GGATC 1 cut(s) 1356
AcoI YGGCCR 2 cut(s) 304, 883
AcsI RAATTY 2 cut(s) 203, 1202
AcuI CTGAAG 2 cut(s) 765, 1163
AfaI GTAC 1 cut(s) 866
AfiI CCNNNNNNNGG 2 cut(s) 341, 547
AflII CTTAAG 1 cut(s) 122
AflIII ACRYGT 1 cut(s) 1171
AgsI TTSAA 7 cut(s) 19, 92, 503, 718, 910, 1019, 1207
AjnI CCWGG 1 cut(s) 1010
AjuI GAANNNNNNNTTGG 2 cut(s) 821, 853
Alw21I GWGCWC 2 cut(s) 1248, 1375
Alw26I GTCTC 1 cut(s) 1001
Alw44I GTGCAC 1 cut(s) 1371
AlwI GGATC 1 cut(s) 1356
AlwNI CAGNNNCTG 2 cut(s) 782, 1571
AoxI GGCC 2 cut(s) 304, 883
ApaLI GTGCAC 1 cut(s) 1371
ApeKI GCWGC 3 cut(s) 844, 1571, 1574
ApoI RAATTY 2 cut(s) 203, 1202
ArsI GACNNNNNNTTYG 1 cut(s) 30
AspS9I GGNCC 2 cut(s) 100, 934
AsuHPI GGTGA 5 cut(s) 155, 324, 667, 1438, 1526
AvaII GGWCC 2 cut(s) 100, 934
BaeGI GKGCMC 2 cut(s) 1375, 1554
BalI TGGCCA 1 cut(s) 306
BanI GGYRCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 1248
Bbv12I GWGCWC 2 cut(s) 1248, 1375
BbvCI CCTCAGC 2 cut(s) 783, 1461
BbvI GCAGC 3 cut(s) 831, 1558, 1561
BceAI ACGGC 2 cut(s) 870, 1272
BciT130I CCWGG 1 cut(s) 1012
BclI TGATCA 1 cut(s) 1294
BcoDI GTCTC 1 cut(s) 1001
BfaI CTAG 4 cut(s) 156, 351, 506, 1046
BfmI CTRYAG 1 cut(s) 845
BfrI CTTAAG 1 cut(s) 122
BglII AGATCT 1 cut(s) 586
BisI GCNGC 3 cut(s) 845, 1572, 1575
BlsI GCNGC 3 cut(s) 846, 1573, 1576
Bme1390I CCNGG 1 cut(s) 1012
Bme18I GGWCC 2 cut(s) 100, 934
BmgT120I GGNCC 2 cut(s) 100, 934
BmiI GGNNCC 1 cut(s) 379
BmrFI CCNGG 1 cut(s) 1012
BmsI GCATC 4 cut(s) 632, 859, 916, 1086
BpmI CTGGAG 3 cut(s) 524, 968, 1523
Bpu10I CCTNAGC 4 cut(s) 340, 783, 1254, 1461
BpuEI CTTGAG 2 cut(s) 671, 914
Bsa29I ATCGAT 1 cut(s) 220
BsaI GGTCTC 1 cut(s) 1001
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 2 cut(s) 341, 547
Bse118I RCCGGY 1 cut(s) 1326
Bse1I ACTGG 3 cut(s) 231, 937, 1506
BseBI CCWGG 1 cut(s) 1012
BseCI ATCGAT 1 cut(s) 220
BseGI GGATG 4 cut(s) 491, 517, 703, 1246
BseLI CCNNNNNNNGG 2 cut(s) 341, 547
BseMII CTCAG 6 cut(s) 354, 402, 597, 723, 774, 1452
BseNI ACTGG 3 cut(s) 231, 937, 1506
BseRI GAGGAG 3 cut(s) 716, 1424, 1478
BseSI GKGCMC 2 cut(s) 1375, 1554
BseXI GCAGC 3 cut(s) 831, 1558, 1561
BseYI CCCAGC 3 cut(s) 230, 437, 1358
BshFI GGCC 2 cut(s) 306, 885
BshNI GGYRCC 1 cut(s) 377
BshVI ATCGAT 1 cut(s) 220
BsiHKAI GWGCWC 2 cut(s) 1248, 1375
BsiSI CCGG 1 cut(s) 1327
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 2 cut(s) 341, 547
BsmAI GTCTC 1 cut(s) 1001
BsmFI GGGAC 1 cut(s) 19
BsmI GAATGC 1 cut(s) 712
BsnI GGCC 2 cut(s) 306, 885
Bso31I GGTCTC 1 cut(s) 1001
Bsp1286I GDGCHC 3 cut(s) 1248, 1375, 1554
Bsp143I GATC 6 cut(s) 221, 556, 586, 889, 1294, 1348
BspANI GGCC 2 cut(s) 306, 885
BspCNI CTCAG 6 cut(s) 353, 403, 598, 724, 775, 1453
BspDI ATCGAT 1 cut(s) 220
BspLI GGNNCC 1 cut(s) 379
BspMAI CTGCAG 1 cut(s) 849
BspPI GGATC 1 cut(s) 1356
BspT107I GGYRCC 1 cut(s) 377
BspTI CTTAAG 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 1001
BsrFI RCCGGY 1 cut(s) 1326
BsrI ACTGG 3 cut(s) 231, 937, 1506
BssAI RCCGGY 1 cut(s) 1326
BssMI GATC 6 cut(s) 221, 556, 586, 889, 1294, 1348
Bst2UI CCWGG 1 cut(s) 1012
Bst6I CTCTTC 3 cut(s) 87, 905, 1416
BstAFI CTTAAG 1 cut(s) 122
BstAPI GCANNNNNTGC 1 cut(s) 1571
BstC8I GCNNGC 2 cut(s) 1375, 1563
BstDEI CTNAG 7 cut(s) 340, 411, 606, 732, 783, 1254, 1461
BstF5I GGATG 4 cut(s) 491, 517, 703, 1246
BstKTI GATC 6 cut(s) 224, 559, 589, 892, 1297, 1351
BstMAI GTCTC 1 cut(s) 1001
BstMBI GATC 6 cut(s) 221, 556, 586, 889, 1294, 1348
BstMWI GCNNNNNNNGC 3 cut(s) 257, 1558, 1571
BstNI CCWGG 1 cut(s) 1012
BstNSI RCATGY 3 cut(s) 836, 964, 1175
BstSCI CCNGG 1 cut(s) 1010
BstSFI CTRYAG 1 cut(s) 845
BstSLI GKGCMC 2 cut(s) 1375, 1554
BstV1I GCAGC 3 cut(s) 831, 1558, 1561
BstX2I RGATCY 2 cut(s) 586, 1348
BstYI RGATCY 2 cut(s) 586, 1348
Bsu15I ATCGAT 1 cut(s) 220
BsuRI GGCC 2 cut(s) 306, 885
BsuTUI ATCGAT 1 cut(s) 220
BtsCI GGATG 4 cut(s) 491, 517, 703, 1246
BtsI GCAGTG 1 cut(s) 783
BtsIMutI CAGTG 3 cut(s) 224, 783, 1499
Cac8I GCNNGC 2 cut(s) 1375, 1563
CaiI CAGNNNCTG 2 cut(s) 782, 1571
Cfr10I RCCGGY 1 cut(s) 1326
Cfr13I GGNCC 2 cut(s) 100, 934
ClaI ATCGAT 1 cut(s) 220
Csp6I GTAC 1 cut(s) 865
CviAII CATG 9 cut(s) 33, 466, 526, 799, 833, 923, 961, 1172, 1343
CviQI GTAC 1 cut(s) 865
DdeI CTNAG 7 cut(s) 340, 411, 606, 732, 783, 1254, 1461
DpnI GATC 6 cut(s) 223, 558, 588, 891, 1296, 1350
DpnII GATC 6 cut(s) 221, 556, 586, 889, 1294, 1348
DraI TTTAAA 1 cut(s) 369
DrdI GACNNNNNNGTC 1 cut(s) 50
DseDI GACNNNNNNGTC 1 cut(s) 50
EaeI YGGCCR 2 cut(s) 304, 883
Eam1104I CTCTTC 3 cut(s) 87, 905, 1416
EarI CTCTTC 3 cut(s) 87, 905, 1416
Ecl136II GAGCTC 1 cut(s) 1246
Eco24I GRGCYC 1 cut(s) 1248
Eco31I GGTCTC 1 cut(s) 1001
Eco32I GATATC 1 cut(s) 1449
Eco47I GGWCC 2 cut(s) 100, 934
Eco53kI GAGCTC 1 cut(s) 1246
Eco57I CTGAAG 2 cut(s) 765, 1163
EcoICRI GAGCTC 1 cut(s) 1246
EcoRII CCWGG 1 cut(s) 1010
EcoRV GATATC 1 cut(s) 1449
EcoT38I GRGCYC 1 cut(s) 1248
FaeI CATG 9 cut(s) 36, 469, 529, 802, 836, 926, 964, 1175, 1346
FalI AAGNNNNNCTT 4 cut(s) 756, 788, 798, 830
FaqI GGGAC 1 cut(s) 19
FatI CATG 9 cut(s) 32, 465, 525, 798, 832, 922, 960, 1171, 1342
FauNDI CATATG 1 cut(s) 264
FbaI TGATCA 1 cut(s) 1294
Fnu4HI GCNGC 3 cut(s) 845, 1572, 1575
FokI GGATG 4 cut(s) 498, 524, 710, 1253
FriOI GRGCYC 1 cut(s) 1248
Fsp4HI GCNGC 3 cut(s) 845, 1572, 1575
FspBI CTAG 4 cut(s) 156, 351, 506, 1046
GluI GCNGC 3 cut(s) 845, 1572, 1575
GsaI CCCAGC 3 cut(s) 234, 441, 1362
GsuI CTGGAG 3 cut(s) 524, 968, 1523
HaeIII GGCC 2 cut(s) 306, 885
HapII CCGG 1 cut(s) 1327
Hin1II CATG 9 cut(s) 36, 469, 529, 802, 836, 926, 964, 1175, 1346
HpaII CCGG 1 cut(s) 1327
HphI GGTGA 5 cut(s) 155, 324, 667, 1438, 1526
Hpy166II GTNNAC 3 cut(s) 1169, 1261, 1373
Hpy188III TCNNGA 8 cut(s) 59, 89, 275, 541, 560, 893, 947, 1046
Hpy8I GTNNAC 3 cut(s) 1169, 1261, 1373
HpyAV CCTTC 4 cut(s) 740, 1138, 1231, 1307
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 1558, 1571
HpyF3I CTNAG 7 cut(s) 340, 411, 606, 732, 783, 1254, 1461
Hsp92II CATG 9 cut(s) 36, 469, 529, 802, 836, 926, 964, 1175, 1346
Ksp22I TGATCA 1 cut(s) 1294
Kzo9I GATC 6 cut(s) 221, 556, 586, 889, 1294, 1348
LmnI GCTCC 4 cut(s) 543, 841, 1084, 1504
Lsp1109I GCAGC 3 cut(s) 831, 1558, 1561
LweI GCATC 4 cut(s) 632, 859, 916, 1086
MaeI CTAG 4 cut(s) 156, 351, 506, 1046
MaeIII GTNAC 4 cut(s) 242, 330, 727, 1329
MalI GATC 6 cut(s) 223, 558, 588, 891, 1296, 1350
MboI GATC 6 cut(s) 221, 556, 586, 889, 1294, 1348
MflI RGATCY 2 cut(s) 586, 1348
MhlI GDGCHC 3 cut(s) 1248, 1375, 1554
MlsI TGGCCA 1 cut(s) 306
MluNI TGGCCA 1 cut(s) 306
MlyI GAGTC 6 cut(s) 265, 416, 422, 470, 625, 812
MmeI TCCRAC 5 cut(s) 6, 591, 645, 675, 783
Mox20I TGGCCA 1 cut(s) 306
MscI TGGCCA 1 cut(s) 306
MseI TTAA 7 cut(s) 111, 123, 194, 368, 426, 753, 1545
MslI CAYNNNNRTG 1 cut(s) 1268
Msp20I TGGCCA 1 cut(s) 306
MspCI CTTAAG 1 cut(s) 122
MspI CCGG 1 cut(s) 1327
MspR9I CCNGG 1 cut(s) 1012
Mva1269I GAATGC 1 cut(s) 712
MvaI CCWGG 1 cut(s) 1012
MwoI GCNNNNNNNGC 3 cut(s) 257, 1558, 1571
NdeI CATATG 1 cut(s) 264
NdeII GATC 6 cut(s) 221, 556, 586, 889, 1294, 1348
NlaIII CATG 9 cut(s) 36, 469, 529, 802, 836, 926, 964, 1175, 1346
NlaIV GGNNCC 1 cut(s) 379
NmuCI GTSAC 2 cut(s) 242, 330
NspI RCATGY 3 cut(s) 836, 964, 1175
PciI ACATGT 1 cut(s) 1171
PcsI WCGNNNNNNNCGW 1 cut(s) 1271
PctI GAATGC 1 cut(s) 712
PfeI GAWTC 8 cut(s) 29, 146, 173, 383, 419, 451, 991, 1042
PkrI GCNGC 3 cut(s) 846, 1573, 1576
PleI GAGTC 6 cut(s) 265, 415, 421, 470, 624, 812
PpsI GAGTC 6 cut(s) 265, 415, 421, 470, 624, 812
PscI ACATGT 1 cut(s) 1171
PsiI TTATAA 1 cut(s) 180
Psp124BI GAGCTC 1 cut(s) 1248
Psp6I CCWGG 1 cut(s) 1010
PspFI CCCAGC 3 cut(s) 230, 437, 1358
PspGI CCWGG 1 cut(s) 1010
PspN4I GGNNCC 1 cut(s) 379
PspPI GGNCC 2 cut(s) 100, 934
PstI CTGCAG 1 cut(s) 849
PstNI CAGNNNCTG 2 cut(s) 782, 1571
PsuI RGATCY 2 cut(s) 586, 1348
RsaI GTAC 1 cut(s) 866
RsaNI GTAC 1 cut(s) 865
RseI CAYNNNNRTG 1 cut(s) 1268
SacI GAGCTC 1 cut(s) 1248
SaqAI TTAA 7 cut(s) 111, 123, 194, 368, 426, 753, 1545
SatI GCNGC 3 cut(s) 845, 1572, 1575
Sau3AI GATC 6 cut(s) 221, 556, 586, 889, 1294, 1348
Sau96I GGNCC 2 cut(s) 100, 934
SchI GAGTC 6 cut(s) 265, 416, 422, 470, 625, 812
ScrFI CCNGG 1 cut(s) 1012
SduI GDGCHC 3 cut(s) 1248, 1375, 1554
SfaNI GCATC 4 cut(s) 632, 859, 916, 1086
SfcI CTRYAG 1 cut(s) 845
SinI GGWCC 2 cut(s) 100, 934
SmiMI CAYNNNNRTG 1 cut(s) 1268
SmlI CTYRAG 3 cut(s) 122, 686, 893
SmoI CTYRAG 3 cut(s) 122, 686, 893
SspMI CTAG 4 cut(s) 156, 351, 506, 1046
SstI GAGCTC 1 cut(s) 1248
StyD4I CCNGG 1 cut(s) 1010
TaqI TCGA 3 cut(s) 220, 449, 561
TfiI GAWTC 8 cut(s) 29, 146, 173, 383, 419, 451, 991, 1042
Tru1I TTAA 7 cut(s) 111, 123, 194, 368, 426, 753, 1545
Tru9I TTAA 7 cut(s) 111, 123, 194, 368, 426, 753, 1545
TscAI CASTG 3 cut(s) 231, 783, 1506
TseFI GTSAC 2 cut(s) 242, 330
TseI GCWGC 3 cut(s) 844, 1571, 1574
Tsp45I GTSAC 2 cut(s) 242, 330
TspDTI ATGAA 6 cut(s) 21, 528, 542, 959, 1068, 1404
TspRI CASTG 3 cut(s) 231, 783, 1506
Vha464I CTTAAG 1 cut(s) 122
VneI GTGCAC 1 cut(s) 1371
VpaK11BI GGWCC 2 cut(s) 100, 934
XapI RAATTY 2 cut(s) 203, 1202
XbaI TCTAGA 1 cut(s) 1045
XceI RCATGY 3 cut(s) 836, 964, 1175
XcmI CCANNNNNNNNNTGG 1 cut(s) 314
XspI CTAG 4 cut(s) 156, 351, 506, 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.