pycom07g25480

Tic22-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012179
Physical Location & Seq
Forward (+)
25780523 .. 25783206
2684 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25480.5

Sequence Viewer

Length: 1644 bp
ATGGCCTCACCATCCGAACCACCCCACACCAAGCAGCAACTCAGACCCACCATAACCCACTTGGTCCACTCCACCACATTCAATCTCCTCTCCCTCTTTGCCTCCCCCAAGACAACGCCGTCGCCATCGCCATCGCCGTTCACGGCTCCTCCGGAAATCCGCGTCGCGCTCTTCCTCCCCAACTTGCCAAAACCACTCGCTTTCCAGCCCGACTTCAAACCCTCCGCCATGAAGGGCGTGGCTTCGTCGCCCGAGTCGACCTCCGGGTTCCCGTCAACGGTCAGGATTGCAGGGCTGAACTCGAATATCAAGGGCAGCGGTGGGCCGGCGTTTGTGGGCCAGGTCTTCAGTATGTGCGACCTCTCCGGGACTGGTCTCATGGCCGTCTCCACCCACTTCGACATCCCCTTTATTTCCAAAAGAACTCCCGAGTGGCTGAAGAAGATGTTTAAATCAATTACTAACAGTGAGAGAAACGGCCCCGTGTTTCGATTTTTCATGGATTTAGGCGATGCAGTTACTTATGTGAAACGACTGAATATTCCGAGTGGCGTGGTGGGAGCTTGTCGTCTCGATTTAGCATATGAACATTTCAAGGAAAAGCCGCACCTATTCCAGTTTGTTCCAAATGAAAAACAGGTCAAGGCAGCTAACAAACTTCTCAAGACAATGCCGCAGAGTGGCAAATGCAAAAGGGTTGACGGAGTTCCGGTTTTTAGTGCTCAAAACTTGGATATTGCAATAGCAAGCTCGGATGGGATCAAATGGTATACTCCCTACTTTTTTGATAAAAACATGCTTGATAACATTCTTGAGGAATCTGTAGATCAGCATTTCCATTCTTTAATTCAAACTCGGCACACGCAGCGGCGACATGATGTGATTGATGACAACATACCCGCAGAAGTAATTGAAGAGATGGGCGATAACTTGTGGGAGCCTCCAGAGGTTCAGGAAGTAATGGATGAGATAGGGAATCCTGGAATACCTTTGAGTGTCATTTCTAAAGCCGCTGAAATGCAGCTCCTTTATGCTGTTGATAAAGTACTTCTGGGTAATAGGTGGCTACGGAAAGCAACAGGCATTCAACCCAAATTCCCTTATATGGTTGACTCTTTCGAAAGAAGGAGTGCAGCCTCTTTTCTTAGAGCTTCTCAGTCAGCCAGATGCCTTACCAATGGGGAGGTAGTAAATGATAACGAAGATTCTCTAGATTGTAGCACTTCGGAGATTAAATTAAAGGATGATATTCACACCAATAAAGGAAACAAAACAGATCGACGGTTTCCTTTCAGTGATTGGTTTAGTCATCTGTGGTCGAAACAAAAGGACCAAAATGAAGAGTTTCCAAAGGAATGCATAGAGCAGAGCACACTGCAAAATCCAAACCTTCCTAAGATTACTATGGTTGGCATTTCAACTGGTGAGGCAGGGCCGATGAGTAAAGCAAATTTAAAGAAGACGATGGAGGATTTAACAAAAGAATTGGAGCAGACGGATTCGGGAAATGCTTTTGGTAGCGATACAGGTAATGAGTTGAGATTTGAAGATAGAGATCCACTGTTTGTGGCAAACGTAGGTGATTATTATTCTGGCATGGCAAAGACGCGTTCAGCTAGGTGGGTTCGTGGAGGAAACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tic22

Protein Analysis

548

Amino Acids

60.97

Weight (kDa)

6.66

Isoelectric Point (pI)

53.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012179)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62650
fragaria_vesca FvH4_7g30450 FvH4_7g30450
malus_domestica MD01G1210100.v1.1 MD07G1280200.v1.1
prunus_persica Prupe.2G302400_v2.0.a1 Prupe.2G302400_v2.0.a1 Prupe.2G302400_v2.0.a1
pyrus_communis pycom07g25480
rosa_chinensis RchiOBHm_Chr1g0378611
rosa_laevigata RLG00000026419
rosa_multiflora Rmu_sc0002395.1_g000010
rosa_roxburghii Rroxscaffold_4G00280360
rosa_rugosa Rorug01G0409900
rosa_samantha Rh1AG431400 Rh1BG389700 Rh1CG403000 Rh1DG420900
rosa_wichuraiana Rw1G037790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 118
AccI GTMKAC 2 cut(s) 257, 770
AccII CGCG 3 cut(s) 162, 167, 1609
AccIII TCCGGA 1 cut(s) 151
AciI CCGC 8 cut(s) 160, 225, 318, 605, 674, 868, 900, 1011
AclWI GGATC 2 cut(s) 767, 1550
AcoI YGGCCR 1 cut(s) 381
AcsI RAATTY 2 cut(s) 1094, 1450
AcuI CTGAAG 2 cut(s) 331, 458
AfaI GTAC 1 cut(s) 1047
AfiI CCNNNNNNNGG 3 cut(s) 277, 680, 1105
AflIII ACRYGT 1 cut(s) 1607
AgsI TTSAA 8 cut(s) 82, 217, 595, 851, 914, 1088, 1419, 1547
AjnI CCWGG 2 cut(s) 339, 979
AluBI AGCT 6 cut(s) 563, 650, 750, 1024, 1151, 1616
AluI AGCT 6 cut(s) 563, 650, 750, 1024, 1151, 1616
Alw21I GWGCWC 2 cut(s) 724, 1373
Alw26I GTCTC 3 cut(s) 380, 391, 575
AlwI GGATC 2 cut(s) 767, 1550
Ama87I CYCGRG 2 cut(s) 251, 428
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 6 cut(s) 3, 323, 337, 381, 478, 1433
ApeKI GCWGC 6 cut(s) 34, 315, 647, 865, 1021, 1133
ApoI RAATTY 2 cut(s) 1094, 1450
Asp700I GAANNNNTTC 1 cut(s) 1344
AspLEI GCGC 1 cut(s) 169
AspS9I GGNCC 6 cut(s) 64, 323, 337, 479, 1330, 1433
AsuC2I CCSGG 2 cut(s) 265, 367
AsuHPI GGTGA 2 cut(s) 1436, 1592
AsuII TTCGAA 1 cut(s) 1119
AvaI CYCGRG 2 cut(s) 251, 428
AvaII GGWCC 2 cut(s) 64, 1330
BbsI GAAGAC 2 cut(s) 337, 1466
Bbv12I GWGCWC 2 cut(s) 724, 1373
BbvI GCAGC 6 cut(s) 46, 327, 659, 877, 1033, 1145
BccI CCATC 6 cut(s) 19, 133, 139, 749, 913, 1459
BceAI ACGGC 5 cut(s) 103, 121, 159, 368, 493
BciT130I CCWGG 2 cut(s) 341, 981
BcnI CCSGG 2 cut(s) 265, 367
BcoDI GTCTC 3 cut(s) 380, 391, 575
BfaI CTAG 2 cut(s) 1211, 1617
BfmI CTRYAG 1 cut(s) 822
BmcAI AGTACT 1 cut(s) 1047
Bme1390I CCNGG 4 cut(s) 265, 341, 367, 981
Bme18I GGWCC 2 cut(s) 64, 1330
BmeT110I CYCGRG 2 cut(s) 251, 428
BmgT120I GGNCC 6 cut(s) 64, 323, 337, 479, 1330, 1433
BmiI GGNNCC 4 cut(s) 147, 269, 481, 939
BmrFI CCNGG 4 cut(s) 265, 341, 367, 981
BmsI GCATC 2 cut(s) 502, 1157
BpiI GAAGAC 2 cut(s) 337, 1466
BplI GAGNNNNNCTC 2 cut(s) 245, 277
BpmI CTGGAG 1 cut(s) 927
Bpu14I TTCGAA 1 cut(s) 1119
BpuEI CTTGAG 2 cut(s) 647, 833
BpuMI CCSGG 2 cut(s) 265, 367
BsaBI GATNNNNATC 1 cut(s) 1554
BsaI GGTCTC 1 cut(s) 380
BsaWI WCCGGW 2 cut(s) 151, 709
BsaXI ACNNNNNCTCC 4 cut(s) 133, 163, 696, 726
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 680, 1105
Bse118I RCCGGY 1 cut(s) 325
Bse1I ACTGG 3 cut(s) 376, 616, 1426
Bse8I GATNNNNATC 1 cut(s) 1554
BseAI TCCGGA 1 cut(s) 151
BseBI CCWGG 2 cut(s) 341, 981
BseGI GGATG 5 cut(s) 11, 402, 760, 970, 1249
BseJI GATNNNNATC 1 cut(s) 1554
BseLI CCNNNNNNNGG 3 cut(s) 277, 680, 1105
BseMII CTCAG 2 cut(s) 55, 1169
BseNI ACTGG 3 cut(s) 376, 616, 1426
BseRI GAGGAG 2 cut(s) 77, 138
BseXI GCAGC 6 cut(s) 46, 327, 659, 877, 1033, 1145
BsgI GTGCAG 1 cut(s) 1152
Bsh1236I CGCG 3 cut(s) 162, 167, 1609
BshFI GGCC 6 cut(s) 5, 325, 339, 383, 480, 1435
BsiHKAI GWGCWC 2 cut(s) 724, 1373
BsiHKCI CYCGRG 2 cut(s) 251, 428
BsiSI CCGG 5 cut(s) 152, 264, 326, 366, 710
BslFI GGGAC 1 cut(s) 382
BslI CCNNNNNNNGG 3 cut(s) 277, 680, 1105
BsmAI GTCTC 3 cut(s) 380, 391, 575
BsmBI CGTCTC 2 cut(s) 391, 575
BsmFI GGGAC 1 cut(s) 382
BsmI GAATGC 2 cut(s) 1083, 1361
BsnI GGCC 6 cut(s) 5, 325, 339, 383, 480, 1435
Bso31I GGTCTC 1 cut(s) 380
BsoBI CYCGRG 2 cut(s) 251, 428
Bsp119I TTCGAA 1 cut(s) 1119
Bsp1286I GDGCHC 2 cut(s) 724, 1373
Bsp13I TCCGGA 1 cut(s) 151
Bsp143I GATC 4 cut(s) 759, 826, 1276, 1555
BspACI CCGC 8 cut(s) 160, 225, 318, 605, 674, 868, 900, 1011
BspANI GGCC 6 cut(s) 5, 325, 339, 383, 480, 1435
BspCNI CTCAG 2 cut(s) 54, 1168
BspEI TCCGGA 1 cut(s) 151
BspFNI CGCG 3 cut(s) 162, 167, 1609
BspLI GGNNCC 4 cut(s) 147, 269, 481, 939
BspPI GGATC 2 cut(s) 767, 1550
BspQI GCTCTTC 1 cut(s) 176
BspT104I TTCGAA 1 cut(s) 1119
BspTNI GGTCTC 1 cut(s) 380
BsrFI RCCGGY 1 cut(s) 325
BsrI ACTGG 3 cut(s) 376, 616, 1426
BssAI RCCGGY 1 cut(s) 325
BssMI GATC 4 cut(s) 759, 826, 1276, 1555
BssNAI GTATAC 1 cut(s) 771
Bst1107I GTATAC 1 cut(s) 771
Bst2UI CCWGG 2 cut(s) 341, 981
Bst4CI ACNGT 4 cut(s) 280, 467, 1284, 1563
Bst6I CTCTTC 3 cut(s) 176, 909, 1335
BstBI TTCGAA 1 cut(s) 1119
BstC8I GCNNGC 2 cut(s) 327, 748
BstDEI CTNAG 4 cut(s) 41, 1145, 1155, 1395
BstF5I GGATG 5 cut(s) 11, 402, 760, 970, 1249
BstFNI CGCG 3 cut(s) 162, 167, 1609
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 4 cut(s) 762, 829, 1279, 1558
BstMAI GTCTC 3 cut(s) 380, 391, 575
BstMBI GATC 4 cut(s) 759, 826, 1276, 1555
BstMWI GCNNNNNNNGC 1 cut(s) 865
BstNI CCWGG 2 cut(s) 341, 981
BstNSI RCATGY 1 cut(s) 799
BstSCI CCNGG 4 cut(s) 263, 339, 365, 979
BstSFI CTRYAG 1 cut(s) 822
BstUI CGCG 3 cut(s) 162, 167, 1609
BstV1I GCAGC 6 cut(s) 46, 327, 659, 877, 1033, 1145
BstV2I GAAGAC 2 cut(s) 337, 1466
BstX2I RGATCY 1 cut(s) 1555
BstYI RGATCY 1 cut(s) 1555
BstZ17I GTATAC 1 cut(s) 771
BsuRI GGCC 6 cut(s) 5, 325, 339, 383, 480, 1435
BtgZI GCGATG 3 cut(s) 111, 117, 525
BtsCI GGATG 5 cut(s) 11, 402, 760, 970, 1249
BtsI GCAGTG 1 cut(s) 1373
BtsIMutI CAGTG 4 cut(s) 472, 1300, 1373, 1559
Cac8I GCNNGC 2 cut(s) 327, 748
CfoI GCGC 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 325
Cfr13I GGNCC 6 cut(s) 64, 323, 337, 479, 1330, 1433
CseI GACGC 2 cut(s) 151, 1615
Csp6I GTAC 1 cut(s) 1046
CviAII CATG 6 cut(s) 229, 379, 499, 796, 875, 1597
CviQI GTAC 1 cut(s) 1046
DdeI CTNAG 4 cut(s) 41, 1145, 1155, 1395
DpnI GATC 4 cut(s) 761, 828, 1278, 1557
DpnII GATC 4 cut(s) 759, 826, 1276, 1555
DraI TTTAAA 2 cut(s) 451, 1455
DrdI GACNNNNNNGTC 1 cut(s) 118
DseDI GACNNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 381
Eam1104I CTCTTC 3 cut(s) 176, 909, 1335
EarI CTCTTC 3 cut(s) 176, 909, 1335
EciI GGCGGA 1 cut(s) 214
Eco31I GGTCTC 1 cut(s) 380
Eco47I GGWCC 2 cut(s) 64, 1330
Eco57I CTGAAG 2 cut(s) 331, 458
Eco88I CYCGRG 2 cut(s) 251, 428
EcoRII CCWGG 2 cut(s) 339, 979
EcoT22I ATGCAT 1 cut(s) 1361
Esp3I CGTCTC 2 cut(s) 391, 575
FaeI CATG 6 cut(s) 232, 382, 502, 799, 878, 1600
FaqI GGGAC 1 cut(s) 382
FatI CATG 6 cut(s) 228, 378, 498, 795, 874, 1596
FauI CCCGC 1 cut(s) 907
FauNDI CATATG 1 cut(s) 583
FblI GTMKAC 2 cut(s) 257, 770
FokI GGATG 4 cut(s) 389, 767, 977, 1256
FspBI CTAG 2 cut(s) 1211, 1617
GlaI GCGC 1 cut(s) 168
GsuI CTGGAG 1 cut(s) 927
HaeIII GGCC 6 cut(s) 5, 325, 339, 383, 480, 1435
HapII CCGG 5 cut(s) 152, 264, 326, 366, 710
HgaI GACGC 2 cut(s) 151, 1615
HhaI GCGC 1 cut(s) 169
Hin1II CATG 6 cut(s) 232, 382, 502, 799, 878, 1600
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HincII GTYRAC 4 cut(s) 258, 276, 700, 1111
HindII GTYRAC 4 cut(s) 258, 276, 700, 1111
HinfI GANTC 6 cut(s) 254, 818, 976, 1112, 1205, 1499
HpaII CCGG 5 cut(s) 152, 264, 326, 366, 710
HphI GGTGA 2 cut(s) 1436, 1592
Hpy166II GTNNAC 7 cut(s) 67, 141, 258, 276, 700, 771, 1111
Hpy188I TCNGA 5 cut(s) 16, 44, 546, 754, 1228
Hpy8I GTNNAC 7 cut(s) 67, 141, 258, 276, 700, 771, 1111
Hpy99I CGWCG 4 cut(s) 124, 167, 250, 1284
HpyAV CCTTC 3 cut(s) 226, 1119, 1400
HpyCH4III ACNGT 4 cut(s) 280, 467, 1284, 1563
HpyCH4IV ACGT 1 cut(s) 1575
HpyCH4V TGCA 8 cut(s) 290, 515, 690, 740, 1021, 1133, 1359, 1378
HpyF10VI GCNNNNNNNGC 1 cut(s) 865
HpyF3I CTNAG 4 cut(s) 41, 1145, 1155, 1395
HpySE526I ACGT 1 cut(s) 1575
Hsp92II CATG 6 cut(s) 232, 382, 502, 799, 878, 1600
HspAI GCGC 1 cut(s) 167
Kpn2I TCCGGA 1 cut(s) 151
KroI GCCGGC 1 cut(s) 325
KroNI GCCGGC 1 cut(s) 327
Kzo9I GATC 4 cut(s) 759, 826, 1276, 1555
LguI GCTCTTC 1 cut(s) 176
LmnI GCTCC 5 cut(s) 151, 560, 937, 1029, 1489
Lsp1109I GCAGC 6 cut(s) 46, 327, 659, 877, 1033, 1145
LweI GCATC 2 cut(s) 502, 1157
MaeI CTAG 2 cut(s) 1211, 1617
MaeII ACGT 1 cut(s) 1575
MaeIII GTNAC 1 cut(s) 517
MalI GATC 4 cut(s) 761, 828, 1278, 1557
MboI GATC 4 cut(s) 759, 826, 1276, 1555
MboII GAAGA 9 cut(s) 163, 337, 451, 454, 926, 1214, 1352, 1471, 1559
MflI RGATCY 1 cut(s) 1555
MhlI GDGCHC 2 cut(s) 724, 1373
MluCI AATT 8 cut(s) 456, 846, 909, 1094, 1235, 1450, 1484, 1639
MluI ACGCGT 1 cut(s) 1607
MlyI GAGTC 2 cut(s) 263, 1106
Mph1103I ATGCAT 1 cut(s) 1361
MroI TCCGGA 1 cut(s) 151
MroNI GCCGGC 1 cut(s) 325
MroXI GAANNNNTTC 1 cut(s) 1344
MseI TTAA 6 cut(s) 450, 845, 1233, 1238, 1454, 1475
MspA1I CMGCKG 3 cut(s) 318, 868, 1013
MspI CCGG 5 cut(s) 152, 264, 326, 366, 710
MspR9I CCNGG 4 cut(s) 265, 341, 367, 981
Mva1269I GAATGC 2 cut(s) 1083, 1361
MvaI CCWGG 2 cut(s) 341, 981
MvnI CGCG 3 cut(s) 162, 167, 1609
MwoI GCNNNNNNNGC 1 cut(s) 865
NaeI GCCGGC 1 cut(s) 327
NciI CCSGG 2 cut(s) 265, 367
NdeI CATATG 1 cut(s) 583
NdeII GATC 4 cut(s) 759, 826, 1276, 1555
NgoMIV GCCGGC 1 cut(s) 325
NlaIII CATG 6 cut(s) 232, 382, 502, 799, 878, 1600
NlaIV GGNNCC 4 cut(s) 147, 269, 481, 939
NmeAIII GCCGAG 1 cut(s) 835
NsiI ATGCAT 1 cut(s) 1361
NspI RCATGY 1 cut(s) 799
NspV TTCGAA 1 cut(s) 1119
PciSI GCTCTTC 1 cut(s) 176
PcsI WCGNNNNNNNCGW 2 cut(s) 134, 254
PctI GAATGC 2 cut(s) 1083, 1361
PdiI GCCGGC 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 1344
PfeI GAWTC 4 cut(s) 818, 976, 1205, 1499
PfoI TCCNGGA 2 cut(s) 365, 979
PleI GAGTC 2 cut(s) 262, 1106
PpsI GAGTC 2 cut(s) 262, 1106
Psp6I CCWGG 2 cut(s) 339, 979
PspGI CCWGG 2 cut(s) 339, 979
PspN4I GGNNCC 4 cut(s) 147, 269, 481, 939
PspPI GGNCC 6 cut(s) 64, 323, 337, 479, 1330, 1433
PsuI RGATCY 1 cut(s) 1555
RsaI GTAC 1 cut(s) 1047
RsaNI GTAC 1 cut(s) 1046
SalI GTCGAC 1 cut(s) 256
SapI GCTCTTC 1 cut(s) 176
SaqAI TTAA 6 cut(s) 450, 845, 1233, 1238, 1454, 1475
Sau3AI GATC 4 cut(s) 759, 826, 1276, 1555
Sau96I GGNCC 6 cut(s) 64, 323, 337, 479, 1330, 1433
ScaI AGTACT 1 cut(s) 1047
SchI GAGTC 2 cut(s) 263, 1106
ScrFI CCNGG 4 cut(s) 265, 341, 367, 981
SduI GDGCHC 2 cut(s) 724, 1373
SfaNI GCATC 2 cut(s) 502, 1157
SfcI CTRYAG 1 cut(s) 822
SfuI TTCGAA 1 cut(s) 1119
SinI GGWCC 2 cut(s) 64, 1330
SmlI CTYRAG 2 cut(s) 662, 812
SmoI CTYRAG 2 cut(s) 662, 812
Sse9I AATT 8 cut(s) 456, 846, 909, 1094, 1235, 1450, 1484, 1639
SsiI CCGC 8 cut(s) 160, 225, 318, 605, 674, 868, 900, 1011
SspI AATATT 1 cut(s) 541
SspMI CTAG 2 cut(s) 1211, 1617
StyD4I CCNGG 4 cut(s) 263, 339, 365, 979
TaaI ACNGT 4 cut(s) 280, 467, 1284, 1563
TaiI ACGT 1 cut(s) 1578
TaqI TCGA 8 cut(s) 257, 302, 399, 490, 573, 1119, 1279, 1319
TasI AATT 8 cut(s) 456, 846, 909, 1094, 1235, 1450, 1484, 1639
TatI WGTACW 1 cut(s) 1045
TauI GCSGC 4 cut(s) 607, 676, 871, 1013
TfiI GAWTC 4 cut(s) 818, 976, 1205, 1499
Tru1I TTAA 6 cut(s) 450, 845, 1233, 1238, 1454, 1475
Tru9I TTAA 6 cut(s) 450, 845, 1233, 1238, 1454, 1475
TscAI CASTG 4 cut(s) 472, 1300, 1380, 1566
TseI GCWGC 6 cut(s) 34, 315, 647, 865, 1021, 1133
TspDTI ATGAA 5 cut(s) 245, 487, 600, 645, 1353
TspGWI ACGGA 3 cut(s) 717, 1084, 1511
TspRI CASTG 4 cut(s) 472, 1300, 1380, 1566
VpaK11BI GGWCC 2 cut(s) 64, 1330
XapI RAATTY 2 cut(s) 1094, 1450
XbaI TCTAGA 1 cut(s) 1210
XceI RCATGY 1 cut(s) 799
XcmI CCANNNNNNNNNTGG 2 cut(s) 58, 235
XmiI GTMKAC 2 cut(s) 257, 770
XmnI GAANNNNTTC 1 cut(s) 1344
XspI CTAG 2 cut(s) 1211, 1617
ZrmI AGTACT 1 cut(s) 1047
Zsp2I ATGCAT 1 cut(s) 1361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.