MD08G1006200.v1.1

Divergent CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0017732
Physical Location & Seq
Reverse (-)
524675 .. 525770
1096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1006200.v1.1.491

Sequence Viewer

Length: 663 bp
ATGAATTATTTTTCTTCCCCTTCTATACCAACACTCTCTCTGTCAGACCAGCAACACCTCCTTGAGAAACTTCAAGTGTTTAACATCCAAGGCCGGGACAAACATGGTCGAAACGTTCTTCGCATCGTCGGAAAGTTCTTTCCAGCTCGAAGCGTGACGAACGAGGCTGTGAACAAGTACTTGGAGGAGAAAATATTCCCAAAGTTAGGGGAAACGGCTTTCTTCTCGGTGGTGTACGTGCACACAGATGTGAATCGCGGTGACAACTTTCCCGGAATTTCAACCCTCCGATCCATCTACGAGGCAATTCCGACCGCCGTCAAGGATCGGCTTCAAGCTGTTTACTTTCTCCACCCGGGATTGCAGGCCAAGCTCTTCCTCGCCACCTTTGGTCGTCTCCTTTTCACCGGAGGGTTGTACAAGAAGCTAAAATATGTGCAACGGCTGGAGTTTCTATGGAACCAGGTGCGGAGGAATGAGGTGGAGGTTCCGGAGTTTGTGCACGATTATGATGAGGAGCTGGAGTACCGTCCGATCATGGACTATGGATTAGAGAGTGATCACCCCAGAGTCTACGAAGCTTCCTCTTTTGCGTCTTCTACGACGTCGTCTATGCTGGATTGGCCTGCCGTTTCCATGTACTCCATGCGTTGTATTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2

Protein Analysis

221

Amino Acids

25.41

Weight (kDa)

7.02

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_2 PF13716 36 - 173 2.1e-35 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017732)

Species Orthologous Gene IDs
malus_domestica MD08G1006200.v1.1 MD15G1005600.v1.1
prunus_persica Prupe.1G358200_v2.0.a1
pyrus_communis pycom15g00400
rosa_chinensis RchiOBHm_Chr6g0303811
rosa_laevigata RLG00000011042
rosa_multiflora Rmu_sc0012878.1_g000003
rosa_roxburghii Rroxscaffold_7G00164390
rosa_rugosa Rorug06G0325000
rosa_samantha Rh6AG439300 Rh6BG463700
rosa_wichuraiana Rw6G038030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 608
AccI GTMKAC 1 cut(s) 573
AccII CGCG 1 cut(s) 258
AccIII TCCGGA 1 cut(s) 490
AciI CCGC 3 cut(s) 258, 315, 469
AclI AACGTT 1 cut(s) 114
AclWI GGATC 2 cut(s) 285, 333
AcsI RAATTY 1 cut(s) 276
AcyI GRCGYC 1 cut(s) 605
AfaI GTAC 5 cut(s) 179, 236, 419, 527, 641
AfiI CCNNNNNNNGG 2 cut(s) 94, 206
AgsI TTSAA 3 cut(s) 74, 282, 335
AjnI CCWGG 1 cut(s) 462
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AleI CACNNNNGTG 1 cut(s) 248
AluBI AGCT 6 cut(s) 146, 338, 373, 427, 520, 581
AluI AGCT 6 cut(s) 146, 338, 373, 427, 520, 581
Alw21I GWGCWC 2 cut(s) 243, 504
Alw26I GTCTC 1 cut(s) 401
Alw44I GTGCAC 2 cut(s) 239, 500
AlwI GGATC 2 cut(s) 285, 333
Ama87I CYCGRG 1 cut(s) 355
Aor13HI TCCGGA 1 cut(s) 490
AoxI GGCC 3 cut(s) 91, 366, 623
ApaLI GTGCAC 2 cut(s) 239, 500
ApoI RAATTY 1 cut(s) 276
Asp700I GAANNNNTTC 1 cut(s) 194
AsuC2I CCSGG 4 cut(s) 95, 273, 356, 357
AsuHPI GGTGA 3 cut(s) 272, 397, 554
AvaI CYCGRG 1 cut(s) 355
BaeGI GKGCMC 2 cut(s) 243, 504
BbsI GAAGAC 1 cut(s) 588
Bbv12I GWGCWC 2 cut(s) 243, 504
BccI CCATC 1 cut(s) 302
BceAI ACGGC 4 cut(s) 231, 302, 458, 614
BciT130I CCWGG 1 cut(s) 464
BclI TGATCA 1 cut(s) 559
BcnI CCSGG 4 cut(s) 95, 273, 356, 357
BcoDI GTCTC 1 cut(s) 401
BfaI CTAG 1 cut(s) 661
BmcAI AGTACT 1 cut(s) 179
Bme1390I CCNGG 5 cut(s) 95, 273, 356, 357, 464
BmeT110I CYCGRG 1 cut(s) 355
BmiI GGNNCC 2 cut(s) 461, 489
BmrFI CCNGG 5 cut(s) 95, 273, 356, 357, 464
BmsI GCATC 1 cut(s) 132
BoxI GACNNNNGTC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 588
BpmI CTGGAG 2 cut(s) 467, 542
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 4 cut(s) 95, 273, 356, 357
BsaAI YACGTR 1 cut(s) 238
BsaBI GATNNNNATC 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 605
BsaJI CCNNGG 2 cut(s) 88, 355
BsaWI WCCGGW 2 cut(s) 407, 490
BsaXI ACNNNNNCTCC 2 cut(s) 509, 539
Bsc4I CCNNNNNNNGG 2 cut(s) 94, 206
Bse8I GATNNNNATC 1 cut(s) 252
BseAI TCCGGA 1 cut(s) 490
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 2 cut(s) 88, 355
BseGI GGATG 1 cut(s) 84
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 94, 206
BseRI GAGGAG 2 cut(s) 200, 530
BseSI GKGCMC 2 cut(s) 243, 504
Bsh1236I CGCG 1 cut(s) 258
Bsh1285I CGRYCG 1 cut(s) 315
BshFI GGCC 3 cut(s) 93, 368, 625
BsiEI CGRYCG 1 cut(s) 315
BsiHKAI GWGCWC 2 cut(s) 243, 504
BsiHKCI CYCGRG 1 cut(s) 355
BsiSI CCGG 5 cut(s) 94, 273, 356, 408, 491
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 94, 206
BsmAI GTCTC 1 cut(s) 401
BsmBI CGTCTC 1 cut(s) 401
BsmFI GGGAC 1 cut(s) 110
BsnI GGCC 3 cut(s) 93, 368, 625
BsoBI CYCGRG 1 cut(s) 355
Bsp1286I GDGCHC 2 cut(s) 243, 504
Bsp13I TCCGGA 1 cut(s) 490
Bsp1407I TGTACA 1 cut(s) 417
Bsp143I GATC 4 cut(s) 290, 325, 534, 559
BspACI CCGC 3 cut(s) 258, 315, 469
BspANI GGCC 3 cut(s) 93, 368, 625
BspEI TCCGGA 1 cut(s) 490
BspFNI CGCG 1 cut(s) 258
BspLI GGNNCC 2 cut(s) 461, 489
BspPI GGATC 2 cut(s) 285, 333
BspQI GCTCTTC 1 cut(s) 380
BsrGI TGTACA 1 cut(s) 417
BssECI CCNNGG 2 cut(s) 88, 355
BssMI GATC 4 cut(s) 290, 325, 534, 559
BssNI GRCGYC 1 cut(s) 605
BssT1I CCWWGG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 1 cut(s) 530
Bst6I CTCTTC 1 cut(s) 380
BstACI GRCGYC 1 cut(s) 605
BstAUI TGTACA 1 cut(s) 417
BstBAI YACGTR 1 cut(s) 238
BstC8I GCNNGC 2 cut(s) 366, 627
BstF5I GGATG 1 cut(s) 84
BstFNI CGCG 1 cut(s) 258
BstKTI GATC 4 cut(s) 293, 328, 537, 562
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 4 cut(s) 290, 325, 534, 559
BstMCI CGRYCG 1 cut(s) 315
BstMWI GCNNNNNNNGC 2 cut(s) 370, 622
BstNI CCWGG 1 cut(s) 464
BstPAI GACNNNNGTC 1 cut(s) 317
BstSCI CCNGG 5 cut(s) 93, 271, 354, 355, 462
BstSLI GKGCMC 2 cut(s) 243, 504
BstUI CGCG 1 cut(s) 258
BstV2I GAAGAC 1 cut(s) 588
BsuRI GGCC 3 cut(s) 93, 368, 625
BtsCI GGATG 1 cut(s) 84
Cac8I GCNNGC 2 cut(s) 366, 627
Cfr9I CCCGGG 1 cut(s) 355
CseI GACGC 1 cut(s) 582
CsiI ACCWGGT 1 cut(s) 462
Csp6I GTAC 5 cut(s) 178, 235, 418, 526, 640
CviAII CATG 4 cut(s) 104, 538, 637, 646
CviQI GTAC 5 cut(s) 178, 235, 418, 526, 640
DpnI GATC 4 cut(s) 292, 327, 536, 561
DpnII GATC 4 cut(s) 290, 325, 534, 559
Eam1104I CTCTTC 1 cut(s) 380
EarI CTCTTC 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 88
Eco88I CYCGRG 1 cut(s) 355
EcoRII CCWGG 1 cut(s) 462
EcoT14I CCWWGG 1 cut(s) 88
ErhI CCWWGG 1 cut(s) 88
Esp3I CGTCTC 1 cut(s) 401
FaeI CATG 4 cut(s) 107, 541, 640, 649
FaqI GGGAC 1 cut(s) 110
FatI CATG 4 cut(s) 103, 537, 636, 645
FbaI TGATCA 1 cut(s) 559
FblI GTMKAC 1 cut(s) 573
FokI GGATG 1 cut(s) 71
FspBI CTAG 1 cut(s) 661
GsuI CTGGAG 2 cut(s) 467, 542
HaeIII GGCC 3 cut(s) 93, 368, 625
HapII CCGG 5 cut(s) 94, 273, 356, 408, 491
HgaI GACGC 1 cut(s) 582
Hin1I GRCGYC 1 cut(s) 605
Hin1II CATG 4 cut(s) 107, 541, 640, 649
HindIII AAGCTT 1 cut(s) 579
HinfI GANTC 2 cut(s) 253, 570
HpaII CCGG 5 cut(s) 94, 273, 356, 408, 491
HphI GGTGA 3 cut(s) 272, 397, 554
Hpy166II GTNNAC 6 cut(s) 172, 235, 241, 343, 502, 574
Hpy188I TCNGA 5 cut(s) 46, 131, 290, 312, 534
Hpy188III TCNNGA 1 cut(s) 491
Hpy8I GTNNAC 6 cut(s) 172, 235, 241, 343, 502, 574
Hpy99I CGWCG 3 cut(s) 131, 607, 610
HpyAV CCTTC 1 cut(s) 30
HpyCH4III ACNGT 1 cut(s) 530
HpyCH4IV ACGT 3 cut(s) 114, 237, 605
HpyCH4V TGCA 4 cut(s) 241, 364, 439, 502
HpyF10VI GCNNNNNNNGC 2 cut(s) 370, 622
HpySE526I ACGT 3 cut(s) 114, 237, 605
Hsp92I GRCGYC 1 cut(s) 605
Hsp92II CATG 4 cut(s) 107, 541, 640, 649
Kpn2I TCCGGA 1 cut(s) 490
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 4 cut(s) 290, 325, 534, 559
LguI GCTCTTC 1 cut(s) 380
LmnI GCTCC 1 cut(s) 517
LweI GCATC 1 cut(s) 132
MabI ACCWGGT 1 cut(s) 462
MaeI CTAG 1 cut(s) 661
MaeII ACGT 3 cut(s) 114, 237, 605
MaeIII GTNAC 2 cut(s) 154, 260
MalI GATC 4 cut(s) 292, 327, 536, 561
MboI GATC 4 cut(s) 290, 325, 534, 559
MboII GAAGA 5 cut(s) 6, 110, 214, 367, 588
MhlI GDGCHC 2 cut(s) 243, 504
MluCI AATT 3 cut(s) 4, 276, 306
MlyI GAGTC 1 cut(s) 579
MmeI TCCRAC 2 cut(s) 109, 335
MroI TCCGGA 1 cut(s) 490
MroXI GAANNNNTTC 1 cut(s) 194
MseI TTAA 1 cut(s) 81
MslI CAYNNNNRTG 3 cut(s) 246, 248, 507
MspI CCGG 5 cut(s) 94, 273, 356, 408, 491
MspR9I CCNGG 5 cut(s) 95, 273, 356, 357, 464
MvaI CCWGG 1 cut(s) 464
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 2 cut(s) 370, 622
NciI CCSGG 4 cut(s) 95, 273, 356, 357
NdeII GATC 4 cut(s) 290, 325, 534, 559
NlaIII CATG 4 cut(s) 107, 541, 640, 649
NlaIV GGNNCC 2 cut(s) 461, 489
NmuCI GTSAC 2 cut(s) 154, 260
OliI CACNNNNGTG 1 cut(s) 248
PciSI GCTCTTC 1 cut(s) 380
PdmI GAANNNNTTC 1 cut(s) 194
PfeI GAWTC 1 cut(s) 253
PflFI GACNNNGTC 1 cut(s) 607
PfoI TCCNGGA 1 cut(s) 271
PleI GAGTC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 578
Ppu21I YACGTR 1 cut(s) 238
PshAI GACNNNNGTC 1 cut(s) 317
Psp1406I AACGTT 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 462
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 2 cut(s) 461, 489
PsyI GACNNNGTC 1 cut(s) 607
RsaI GTAC 5 cut(s) 179, 236, 419, 527, 641
RsaNI GTAC 5 cut(s) 178, 235, 418, 526, 640
RseI CAYNNNNRTG 3 cut(s) 246, 248, 507
SapI GCTCTTC 1 cut(s) 380
SaqAI TTAA 1 cut(s) 81
Sau3AI GATC 4 cut(s) 290, 325, 534, 559
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 1 cut(s) 579
ScrFI CCNGG 5 cut(s) 95, 273, 356, 357, 464
SduI GDGCHC 2 cut(s) 243, 504
SexAI ACCWGGT 1 cut(s) 462
SfaNI GCATC 1 cut(s) 132
SmaI CCCGGG 1 cut(s) 357
SmiMI CAYNNNNRTG 3 cut(s) 246, 248, 507
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 3 cut(s) 4, 276, 306
SsiI CCGC 3 cut(s) 258, 315, 469
SspI AATATT 1 cut(s) 195
SspMI CTAG 1 cut(s) 661
StyD4I CCNGG 5 cut(s) 93, 271, 354, 355, 462
StyI CCWWGG 1 cut(s) 88
TaaI ACNGT 1 cut(s) 530
TaiI ACGT 3 cut(s) 117, 240, 608
TaqI TCGA 2 cut(s) 109, 148
TasI AATT 3 cut(s) 4, 276, 306
TatI WGTACW 3 cut(s) 177, 417, 639
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TseFI GTSAC 2 cut(s) 154, 260
Tsp45I GTSAC 2 cut(s) 154, 260
TspDTI ATGAA 1 cut(s) 17
TspMI CCCGGG 1 cut(s) 355
Tth111I GACNNNGTC 1 cut(s) 607
VneI GTGCAC 2 cut(s) 239, 500
XapI RAATTY 1 cut(s) 276
XmaI CCCGGG 1 cut(s) 355
XmiI GTMKAC 1 cut(s) 573
XmnI GAANNNNTTC 1 cut(s) 194
XspI CTAG 1 cut(s) 661
ZraI GACGTC 1 cut(s) 606
ZrmI AGTACT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.