MD15G1005600.v1.1

Divergent CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017732
Physical Location & Seq
Reverse (-)
326934 .. 328341
1408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1005600.v1.1.491

Sequence Viewer

Length: 663 bp
ATGAACTATTCTTCTCCTCCTTACATACCAACAATCTCAATATCGGACCAGCAACACCTCCTTGAGAAACTTCAAGTCTTTAAGATTCAAGGCCGGGACAAACATGGTCGAAAGGTTCTTCGCATCGTCGGAAAGTTCTTTCCAGCTCGAAGCGTGAGCAACGAGGCTGTGAACAAGTACTTGGAGGAGAAAATATTCCCAAAGTTAGGTGACAAAGCCTTCTTCTCGGTGGTGTACGTTCACACAGATGTGAATCGCGGCGACAACTTTCCCGGAATTTCAACCCTGCGATCCATCTACGGGGCAATTCCGACCGCCGTCAAGGATCGCCTCCAAGCCGTTTACTTTCTCCACCCAGGATTGCAGGCCAAGCTCTTCCTTGCCACCTTTGGTCGTCTCCTTTTCACCGGAGGGTTGTACAAGAAGCTAAAGTATGTGACAAGGCTGGAGTTTCTGTGGGTTCAGGTGCGGAGGAATGAGGTGGAAGTTCCGGATTTCGTGTACGATTACGATGAGCAGCTGGAGTACCGTCCGATCATGGACTATGGATTAGAGAGTGATCACCCCAGAGTCTACGGGCCTTCCTCCTTTGCGTCTTCTACGACATCGTCCTTGCTGGATTCGCCTGCTGTGTCCATGTACTCCATGCGTTGTATTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2

Protein Analysis

221

Amino Acids

25.19

Weight (kDa)

9.26

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_2 PF13716 36 - 172 2.3e-36 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017732)

Species Orthologous Gene IDs
malus_domestica MD08G1006200.v1.1 MD15G1005600.v1.1
prunus_persica Prupe.1G358200_v2.0.a1
pyrus_communis pycom15g00400
rosa_chinensis RchiOBHm_Chr6g0303811
rosa_laevigata RLG00000011042
rosa_multiflora Rmu_sc0012878.1_g000003
rosa_roxburghii Rroxscaffold_7G00164390
rosa_rugosa Rorug06G0325000
rosa_samantha Rh6AG439300 Rh6BG463700
rosa_wichuraiana Rw6G038030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 573
AccII CGCG 1 cut(s) 258
AccIII TCCGGA 1 cut(s) 490
AciI CCGC 3 cut(s) 258, 315, 469
AclWI GGATC 2 cut(s) 285, 333
AcsI RAATTY 1 cut(s) 276
AfaI GTAC 6 cut(s) 179, 236, 419, 503, 527, 641
AfiI CCNNNNNNNGG 2 cut(s) 206, 300
AgsI TTSAA 3 cut(s) 74, 89, 282
AjnI CCWGG 1 cut(s) 355
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AleI CACNNNNGTG 1 cut(s) 248
AluBI AGCT 4 cut(s) 146, 373, 427, 520
AluI AGCT 4 cut(s) 146, 373, 427, 520
Alw26I GTCTC 1 cut(s) 401
AlwI GGATC 2 cut(s) 285, 333
Aor13HI TCCGGA 1 cut(s) 490
AoxI GGCC 3 cut(s) 91, 366, 578
ApeKI GCWGC 1 cut(s) 517
ApoI RAATTY 1 cut(s) 276
Asp700I GAANNNNTTC 1 cut(s) 194
AspS9I GGNCC 2 cut(s) 46, 578
AsuC2I CCSGG 2 cut(s) 95, 273
AsuHPI GGTGA 3 cut(s) 221, 397, 554
AvaII GGWCC 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 588
BbvI GCAGC 1 cut(s) 529
BccI CCATC 1 cut(s) 302
BceAI ACGGC 2 cut(s) 302, 323
BciT130I CCWGG 1 cut(s) 357
BclI TGATCA 1 cut(s) 559
BcnI CCSGG 2 cut(s) 95, 273
BcoDI GTCTC 1 cut(s) 401
BfaI CTAG 1 cut(s) 661
BisI GCNGC 2 cut(s) 259, 518
BlsI GCNGC 2 cut(s) 260, 519
BmcAI AGTACT 1 cut(s) 179
Bme1390I CCNGG 3 cut(s) 95, 273, 357
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 46, 578
BmrFI CCNGG 3 cut(s) 95, 273, 357
BmsI GCATC 1 cut(s) 132
BoxI GACNNNNGTC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 588
BpmI CTGGAG 2 cut(s) 467, 542
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 2 cut(s) 95, 273
BsaBI GATNNNNATC 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 355
BsaWI WCCGGW 2 cut(s) 407, 490
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 300
Bse8I GATNNNNATC 1 cut(s) 252
BseAI TCCGGA 1 cut(s) 490
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 1 cut(s) 355
BseJI GATNNNNATC 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 206, 300
BseRI GAGGAG 2 cut(s) 6, 200
BseXI GCAGC 1 cut(s) 529
Bsh1236I CGCG 1 cut(s) 258
Bsh1285I CGRYCG 1 cut(s) 315
BshFI GGCC 3 cut(s) 93, 368, 580
BsiEI CGRYCG 1 cut(s) 315
BsiSI CCGG 4 cut(s) 94, 273, 408, 491
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 206, 300
BsmAI GTCTC 1 cut(s) 401
BsmBI CGTCTC 1 cut(s) 401
BsmFI GGGAC 1 cut(s) 110
BsnI GGCC 3 cut(s) 93, 368, 580
Bsp13I TCCGGA 1 cut(s) 490
Bsp1407I TGTACA 1 cut(s) 417
Bsp143I GATC 4 cut(s) 290, 325, 534, 559
BspACI CCGC 3 cut(s) 258, 315, 469
BspANI GGCC 3 cut(s) 93, 368, 580
BspEI TCCGGA 1 cut(s) 490
BspFNI CGCG 1 cut(s) 258
BspPI GGATC 2 cut(s) 285, 333
BspQI GCTCTTC 1 cut(s) 380
BsrGI TGTACA 1 cut(s) 417
BssECI CCNNGG 1 cut(s) 355
BssMI GATC 4 cut(s) 290, 325, 534, 559
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 1 cut(s) 530
Bst6I CTCTTC 1 cut(s) 380
BstAUI TGTACA 1 cut(s) 417
BstC8I GCNNGC 2 cut(s) 366, 627
BstFNI CGCG 1 cut(s) 258
BstKTI GATC 4 cut(s) 293, 328, 537, 562
BstMAI GTCTC 1 cut(s) 401
BstMBI GATC 4 cut(s) 290, 325, 534, 559
BstMCI CGRYCG 1 cut(s) 315
BstMWI GCNNNNNNNGC 2 cut(s) 370, 622
BstNI CCWGG 1 cut(s) 357
BstPAI GACNNNNGTC 1 cut(s) 317
BstSCI CCNGG 3 cut(s) 93, 271, 355
BstUI CGCG 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 529
BstV2I GAAGAC 1 cut(s) 588
BsuRI GGCC 3 cut(s) 93, 368, 580
Cac8I GCNNGC 2 cut(s) 366, 627
Cfr13I GGNCC 2 cut(s) 46, 578
CseI GACGC 1 cut(s) 582
Csp6I GTAC 6 cut(s) 178, 235, 418, 502, 526, 640
CviAII CATG 4 cut(s) 104, 538, 637, 646
CviQI GTAC 6 cut(s) 178, 235, 418, 502, 526, 640
DpnI GATC 4 cut(s) 292, 327, 536, 561
DpnII GATC 4 cut(s) 290, 325, 534, 559
Eam1104I CTCTTC 1 cut(s) 380
EarI CTCTTC 1 cut(s) 380
Eco47I GGWCC 1 cut(s) 46
EcoRII CCWGG 1 cut(s) 355
Esp3I CGTCTC 1 cut(s) 401
FaeI CATG 4 cut(s) 107, 541, 640, 649
FaiI YATR 7 cut(s) 26, 105, 435, 539, 546, 638, 647
FaqI GGGAC 1 cut(s) 110
FatI CATG 4 cut(s) 103, 537, 636, 645
FbaI TGATCA 1 cut(s) 559
FblI GTMKAC 1 cut(s) 573
Fnu4HI GCNGC 2 cut(s) 259, 518
Fsp4HI GCNGC 2 cut(s) 259, 518
FspBI CTAG 1 cut(s) 661
GluI GCNGC 2 cut(s) 259, 518
GsuI CTGGAG 2 cut(s) 467, 542
HaeIII GGCC 3 cut(s) 93, 368, 580
HapII CCGG 4 cut(s) 94, 273, 408, 491
HgaI GACGC 1 cut(s) 582
Hin1II CATG 4 cut(s) 107, 541, 640, 649
HinfI GANTC 4 cut(s) 85, 253, 570, 620
HpaII CCGG 4 cut(s) 94, 273, 408, 491
HphI GGTGA 3 cut(s) 221, 397, 554
Hpy166II GTNNAC 6 cut(s) 172, 235, 241, 343, 502, 574
Hpy188I TCNGA 4 cut(s) 46, 131, 312, 534
Hpy188III TCNNGA 1 cut(s) 491
Hpy8I GTNNAC 6 cut(s) 172, 235, 241, 343, 502, 574
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 2 cut(s) 229, 591
HpyCH4III ACNGT 1 cut(s) 530
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 1 cut(s) 364
HpyF10VI GCNNNNNNNGC 2 cut(s) 370, 622
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 4 cut(s) 107, 541, 640, 649
Kpn2I TCCGGA 1 cut(s) 490
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 4 cut(s) 290, 325, 534, 559
LguI GCTCTTC 1 cut(s) 380
Lsp1109I GCAGC 1 cut(s) 529
LweI GCATC 1 cut(s) 132
MaeI CTAG 1 cut(s) 661
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 2 cut(s) 209, 436
MalI GATC 4 cut(s) 292, 327, 536, 561
MboI GATC 4 cut(s) 290, 325, 534, 559
MboII GAAGA 5 cut(s) 3, 110, 214, 367, 588
MluCI AATT 2 cut(s) 276, 306
MlyI GAGTC 1 cut(s) 579
MmeI TCCRAC 2 cut(s) 109, 335
MnlI CCTC 9 cut(s) 27, 68, 157, 178, 341, 404, 465, 472, 595
MroI TCCGGA 1 cut(s) 490
MroXI GAANNNNTTC 1 cut(s) 194
MseI TTAA 1 cut(s) 81
MslI CAYNNNNRTG 2 cut(s) 246, 248
MspA1I CMGCKG 1 cut(s) 520
MspI CCGG 4 cut(s) 94, 273, 408, 491
MspR9I CCNGG 3 cut(s) 95, 273, 357
MvaI CCWGG 1 cut(s) 357
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 2 cut(s) 370, 622
NciI CCSGG 2 cut(s) 95, 273
NdeII GATC 4 cut(s) 290, 325, 534, 559
NlaIII CATG 4 cut(s) 107, 541, 640, 649
NmuCI GTSAC 2 cut(s) 209, 436
OliI CACNNNNGTG 1 cut(s) 248
PciSI GCTCTTC 1 cut(s) 380
PdmI GAANNNNTTC 1 cut(s) 194
PfeI GAWTC 3 cut(s) 85, 253, 620
PflFI GACNNNGTC 1 cut(s) 607
PfoI TCCNGGA 1 cut(s) 271
PkrI GCNGC 2 cut(s) 260, 519
PleI GAGTC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 578
PshAI GACNNNNGTC 1 cut(s) 317
Psp6I CCWGG 1 cut(s) 355
PspGI CCWGG 1 cut(s) 355
PspPI GGNCC 2 cut(s) 46, 578
PsyI GACNNNGTC 1 cut(s) 607
PvuII CAGCTG 1 cut(s) 520
RsaI GTAC 6 cut(s) 179, 236, 419, 503, 527, 641
RsaNI GTAC 6 cut(s) 178, 235, 418, 502, 526, 640
RseI CAYNNNNRTG 2 cut(s) 246, 248
SapI GCTCTTC 1 cut(s) 380
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 2 cut(s) 259, 518
Sau3AI GATC 4 cut(s) 290, 325, 534, 559
Sau96I GGNCC 2 cut(s) 46, 578
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 1 cut(s) 579
ScrFI CCNGG 3 cut(s) 95, 273, 357
SfaNI GCATC 1 cut(s) 132
SinI GGWCC 1 cut(s) 46
SmiMI CAYNNNNRTG 2 cut(s) 246, 248
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 2 cut(s) 276, 306
SsiI CCGC 3 cut(s) 258, 315, 469
SspI AATATT 1 cut(s) 195
SspMI CTAG 1 cut(s) 661
StyD4I CCNGG 3 cut(s) 93, 271, 355
TaaI ACNGT 1 cut(s) 530
TaiI ACGT 1 cut(s) 240
TaqI TCGA 2 cut(s) 109, 148
TasI AATT 2 cut(s) 276, 306
TatI WGTACW 3 cut(s) 177, 417, 639
TauI GCSGC 1 cut(s) 261
TfiI GAWTC 3 cut(s) 85, 253, 620
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TseFI GTSAC 2 cut(s) 209, 436
TseI GCWGC 1 cut(s) 517
Tsp45I GTSAC 2 cut(s) 209, 436
TspDTI ATGAA 1 cut(s) 17
Tth111I GACNNNGTC 1 cut(s) 607
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 573
XmnI GAANNNNTTC 1 cut(s) 194
XspI CTAG 1 cut(s) 661
ZrmI AGTACT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.