Rh6AG439300

Divergent CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0017732
Physical Location & Seq
Forward (+)
62748504 .. 62749727
1224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG439300.1

Sequence Viewer

Length: 627 bp
ATGGACTTTCCCTCTGCAGCACTCTCTCTATCAGATCAACAGCAAATGAAGGTGATAGAGAAACTAGAAGTTTTCAAGATCCAGGGCCGAGACAAGCTTGGCCGAAAGGTTCTTCGCATCGTCGGAAAGTCTCTTCCAGCTCGTAGTGCAAGCAAAGAGGCTCTGAATGAGTACTTGAAGGAGAAAATCTTACCAAAATTAGAGGAAAAGCCGTTTTCAATCGTCTACGTGCACACTGATGTTAATCGCAGCGAAAACTTCCCCGGAATTTCTACCCTCCGATCTATCTACGAGGCTATTCCGGTGAACGTGAAGGATAATCTCGAAGCTGTCTACTTTGTCCACCCGGGCTTGCAGGCCAGATTCTTCCTCGCCACCTTTGGCCGTCTCCTCTTCACCGGAGGGTTGTACAGGAAGGTGAACTATGTGACGAGGCTGGAGTTTCTGTGGGATCACGTGAGGCGGAATGAAATGGAGATACCAGAGTTTGTGTACGATCACGATGAGGAGCTGGAGTACCGTCCGATGATGGACTATGGGATAGAGAGTGACCATCCTAGAGTGTATGGTGCACCACCTGCTACGGACTCTTCCGTATCCATCTACTCCATGCGCTGTATCGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2

Protein Analysis

208

Amino Acids

24.03

Weight (kDa)

6.54

Isoelectric Point (pI)

36.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_2 PF13716 34 - 170 2.2e-38 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017732)

Species Orthologous Gene IDs
malus_domestica MD08G1006200.v1.1 MD15G1005600.v1.1
prunus_persica Prupe.1G358200_v2.0.a1
pyrus_communis pycom15g00400
rosa_chinensis RchiOBHm_Chr6g0303811
rosa_laevigata RLG00000011042
rosa_multiflora Rmu_sc0012878.1_g000003
rosa_roxburghii Rroxscaffold_7G00164390
rosa_rugosa Rorug06G0325000
rosa_samantha Rh6AG439300 Rh6BG463700
rosa_wichuraiana Rw6G038030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 586
Acc36I ACCTGC 1 cut(s) 586
AccI GTMKAC 2 cut(s) 225, 333
AciI CCGC 1 cut(s) 463
AclWI GGATC 2 cut(s) 73, 459
AcoI YGGCCR 2 cut(s) 100, 382
AcsI RAATTY 1 cut(s) 267
AcvI CACGTG 1 cut(s) 457
AfaI GTAC 4 cut(s) 173, 410, 494, 518
AgsI TTSAA 3 cut(s) 76, 178, 219
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 4 cut(s) 97, 140, 329, 511
AluI AGCT 4 cut(s) 97, 140, 329, 511
Alw21I GWGCWC 2 cut(s) 234, 574
Alw26I GTCTC 3 cut(s) 84, 135, 392
Alw44I GTGCAC 2 cut(s) 230, 570
AlwI GGATC 2 cut(s) 73, 459
Ama87I CYCGRG 1 cut(s) 346
AoxI GGCC 4 cut(s) 85, 100, 357, 382
ApaLI GTGCAC 2 cut(s) 230, 570
ApeKI GCWGC 2 cut(s) 17, 249
ApoI RAATTY 1 cut(s) 267
AspLEI GCGC 1 cut(s) 615
AspS9I GGNCC 1 cut(s) 85
AsuC2I CCSGG 3 cut(s) 264, 347, 348
AsuHPI GGTGA 4 cut(s) 64, 316, 388, 430
AvaI CYCGRG 1 cut(s) 346
BaeGI GKGCMC 2 cut(s) 234, 574
BaeI ACNNNNGTAYC 2 cut(s) 470, 503
BbrPI CACGTG 1 cut(s) 457
Bbv12I GWGCWC 2 cut(s) 234, 574
BbvI GCAGC 2 cut(s) 29, 261
BccI CCATC 3 cut(s) 523, 561, 608
BceAI ACGGC 2 cut(s) 196, 369
BciT130I CCWGG 1 cut(s) 83
BciVI GTATCC 1 cut(s) 607
BcnI CCSGG 3 cut(s) 264, 347, 348
BcoDI GTCTC 3 cut(s) 84, 135, 392
BfaI CTAG 2 cut(s) 65, 558
BfmI CTRYAG 1 cut(s) 15
BfuAI ACCTGC 1 cut(s) 586
BfuI GTATCC 1 cut(s) 607
BisI GCNGC 2 cut(s) 18, 250
BlsI GCNGC 2 cut(s) 19, 251
BmcAI AGTACT 1 cut(s) 173
Bme1390I CCNGG 4 cut(s) 83, 264, 347, 348
BmeT110I CYCGRG 1 cut(s) 346
BmgT120I GGNCC 1 cut(s) 85
BmrFI CCNGG 4 cut(s) 83, 264, 347, 348
BmsI GCATC 1 cut(s) 126
BpmI CTGGAG 2 cut(s) 458, 533
BpuMI CCSGG 3 cut(s) 264, 347, 348
BsaAI YACGTR 2 cut(s) 229, 457
BsaBI GATNNNNATC 1 cut(s) 243
BsaJI CCNNGG 3 cut(s) 82, 262, 346
BsaWI WCCGGW 2 cut(s) 301, 398
BsaXI ACNNNNNCTCC 4 cut(s) 431, 461, 500, 530
Bse8I GATNNNNATC 1 cut(s) 243
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 3 cut(s) 82, 262, 346
BseGI GGATG 1 cut(s) 553
BseJI GATNNNNATC 1 cut(s) 243
BseRI GAGGAG 2 cut(s) 380, 521
BseSI GKGCMC 2 cut(s) 234, 574
BseXI GCAGC 2 cut(s) 29, 261
BshFI GGCC 4 cut(s) 87, 102, 359, 384
BsiHKAI GWGCWC 2 cut(s) 234, 574
BsiHKCI CYCGRG 1 cut(s) 346
BsiSI CCGG 4 cut(s) 264, 302, 347, 399
BsmAI GTCTC 3 cut(s) 84, 135, 392
BsmBI CGTCTC 1 cut(s) 392
BsnI GGCC 4 cut(s) 87, 102, 359, 384
BsoBI CYCGRG 1 cut(s) 346
Bsp1286I GDGCHC 2 cut(s) 234, 574
Bsp1407I TGTACA 1 cut(s) 408
Bsp143I GATC 5 cut(s) 34, 78, 281, 451, 496
BspACI CCGC 1 cut(s) 463
BspANI GGCC 4 cut(s) 87, 102, 359, 384
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 586
BspPI GGATC 2 cut(s) 73, 459
BsrGI TGTACA 1 cut(s) 408
BssECI CCNNGG 3 cut(s) 82, 262, 346
BssMI GATC 5 cut(s) 34, 78, 281, 451, 496
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 521
Bst6I CTCTTC 3 cut(s) 138, 398, 595
BstAPI GCANNNNNTGC 1 cut(s) 578
BstAUI TGTACA 1 cut(s) 408
BstBAI YACGTR 2 cut(s) 229, 457
BstC8I GCNNGC 3 cut(s) 151, 353, 357
BstF5I GGATG 1 cut(s) 553
BstHHI GCGC 1 cut(s) 615
BstKTI GATC 5 cut(s) 37, 81, 284, 454, 499
BstMAI GTCTC 3 cut(s) 84, 135, 392
BstMBI GATC 5 cut(s) 34, 78, 281, 451, 496
BstMWI GCNNNNNNNGC 2 cut(s) 146, 578
BstNI CCWGG 1 cut(s) 83
BstSCI CCNGG 4 cut(s) 81, 262, 345, 346
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 2 cut(s) 234, 574
BstV1I GCAGC 2 cut(s) 29, 261
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
BsuI GTATCC 1 cut(s) 607
BsuRI GGCC 4 cut(s) 87, 102, 359, 384
BtsCI GGATG 1 cut(s) 553
BtsIMutI CAGTG 1 cut(s) 234
BveI ACCTGC 1 cut(s) 586
Cac8I GCNNGC 3 cut(s) 151, 353, 357
CfoI GCGC 1 cut(s) 615
Cfr13I GGNCC 1 cut(s) 85
Cfr9I CCCGGG 1 cut(s) 346
Csp6I GTAC 4 cut(s) 172, 409, 493, 517
CviAII CATG 1 cut(s) 610
CviQI GTAC 4 cut(s) 172, 409, 493, 517
DpnI GATC 5 cut(s) 36, 80, 283, 453, 498
DpnII GATC 5 cut(s) 34, 78, 281, 451, 496
EaeI YGGCCR 2 cut(s) 100, 382
Eam1104I CTCTTC 3 cut(s) 138, 398, 595
EarI CTCTTC 3 cut(s) 138, 398, 595
EciI GGCGGA 1 cut(s) 478
Eco72I CACGTG 1 cut(s) 457
Eco88I CYCGRG 1 cut(s) 346
EcoRII CCWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 392
FaeI CATG 1 cut(s) 613
FaiI YATR 4 cut(s) 426, 537, 567, 611
FatI CATG 1 cut(s) 609
FblI GTMKAC 2 cut(s) 225, 333
Fnu4HI GCNGC 2 cut(s) 18, 250
FokI GGATG 1 cut(s) 540
Fsp4HI GCNGC 2 cut(s) 18, 250
FspBI CTAG 2 cut(s) 65, 558
GlaI GCGC 1 cut(s) 614
GluI GCNGC 2 cut(s) 18, 250
GsuI CTGGAG 2 cut(s) 458, 533
HaeIII GGCC 4 cut(s) 87, 102, 359, 384
HapII CCGG 4 cut(s) 264, 302, 347, 399
HhaI GCGC 1 cut(s) 615
Hin1II CATG 1 cut(s) 613
Hin6I GCGC 1 cut(s) 613
HinP1I GCGC 1 cut(s) 613
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 2 cut(s) 363, 587
HpaII CCGG 4 cut(s) 264, 302, 347, 399
HphI GGTGA 4 cut(s) 64, 316, 388, 430
Hpy166II GTNNAC 8 cut(s) 226, 232, 307, 334, 343, 421, 493, 572
Hpy188I TCNGA 6 cut(s) 34, 125, 165, 281, 525, 623
Hpy188III TCNNGA 3 cut(s) 76, 323, 500
Hpy8I GTNNAC 8 cut(s) 226, 232, 307, 334, 343, 421, 493, 572
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 4 cut(s) 43, 172, 307, 409
HpyCH4III ACNGT 1 cut(s) 521
HpyCH4IV ACGT 3 cut(s) 228, 309, 456
HpyCH4V TGCA 5 cut(s) 17, 149, 232, 355, 572
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 578
HpySE526I ACGT 3 cut(s) 228, 309, 456
Hsp92II CATG 1 cut(s) 613
HspAI GCGC 1 cut(s) 613
Kzo9I GATC 5 cut(s) 34, 78, 281, 451, 496
LmnI GCTCC 1 cut(s) 508
Lsp1109I GCAGC 2 cut(s) 29, 261
LweI GCATC 1 cut(s) 126
MaeI CTAG 2 cut(s) 65, 558
MaeII ACGT 3 cut(s) 228, 309, 456
MaeIII GTNAC 2 cut(s) 427, 548
MalI GATC 5 cut(s) 36, 80, 283, 453, 498
MboI GATC 5 cut(s) 34, 78, 281, 451, 496
MboII GAAGA 5 cut(s) 104, 125, 358, 385, 582
MflI RGATCY 1 cut(s) 78
MhlI GDGCHC 2 cut(s) 234, 574
MluCI AATT 2 cut(s) 197, 267
MlyI GAGTC 1 cut(s) 581
MmeI TCCRAC 1 cut(s) 103
MseI TTAA 1 cut(s) 243
MslI CAYNNNNRTG 1 cut(s) 237
MspI CCGG 4 cut(s) 264, 302, 347, 399
MspR9I CCNGG 4 cut(s) 83, 264, 347, 348
MvaI CCWGG 1 cut(s) 83
MwoI GCNNNNNNNGC 2 cut(s) 146, 578
NciI CCSGG 3 cut(s) 264, 347, 348
NdeII GATC 5 cut(s) 34, 78, 281, 451, 496
NlaIII CATG 1 cut(s) 613
NmeAIII GCCGAG 1 cut(s) 113
NmuCI GTSAC 2 cut(s) 427, 548
PaqCI CACCTGC 1 cut(s) 586
PfeI GAWTC 1 cut(s) 363
PkrI GCNGC 2 cut(s) 19, 251
PleI GAGTC 1 cut(s) 581
PmaCI CACGTG 1 cut(s) 457
PmlI CACGTG 1 cut(s) 457
PpsI GAGTC 1 cut(s) 581
Ppu21I YACGTR 2 cut(s) 229, 457
Psp6I CCWGG 1 cut(s) 81
PspCI CACGTG 1 cut(s) 457
PspGI CCWGG 1 cut(s) 81
PspPI GGNCC 1 cut(s) 85
PstI CTGCAG 1 cut(s) 19
PsuI RGATCY 1 cut(s) 78
RsaI GTAC 4 cut(s) 173, 410, 494, 518
RsaNI GTAC 4 cut(s) 172, 409, 493, 517
RseI CAYNNNNRTG 1 cut(s) 237
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 2 cut(s) 18, 250
Sau3AI GATC 5 cut(s) 34, 78, 281, 451, 496
Sau96I GGNCC 1 cut(s) 85
ScaI AGTACT 1 cut(s) 173
SchI GAGTC 1 cut(s) 581
ScrFI CCNGG 4 cut(s) 83, 264, 347, 348
SduI GDGCHC 2 cut(s) 234, 574
SfaNI GCATC 1 cut(s) 126
SfcI CTRYAG 1 cut(s) 15
SmaI CCCGGG 1 cut(s) 348
SmiMI CAYNNNNRTG 1 cut(s) 237
Sse9I AATT 2 cut(s) 197, 267
SsiI CCGC 1 cut(s) 463
SspMI CTAG 2 cut(s) 65, 558
StyD4I CCNGG 4 cut(s) 81, 262, 345, 346
TaaI ACNGT 1 cut(s) 521
TaiI ACGT 3 cut(s) 231, 312, 459
TaqI TCGA 1 cut(s) 324
TasI AATT 2 cut(s) 197, 267
TatI WGTACW 2 cut(s) 171, 408
TfiI GAWTC 1 cut(s) 363
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 1 cut(s) 241
TseFI GTSAC 2 cut(s) 427, 548
TseI GCWGC 2 cut(s) 17, 249
Tsp45I GTSAC 2 cut(s) 427, 548
TspDTI ATGAA 2 cut(s) 62, 483
TspGWI ACGGA 2 cut(s) 583, 599
TspMI CCCGGG 1 cut(s) 346
TspRI CASTG 1 cut(s) 241
VneI GTGCAC 2 cut(s) 230, 570
XapI RAATTY 1 cut(s) 267
XmaI CCCGGG 1 cut(s) 346
XmiI GTMKAC 2 cut(s) 225, 333
XspI CTAG 2 cut(s) 65, 558
ZrmI AGTACT 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.