Rorug06G0325000

Divergent CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0017732
Physical Location & Seq
Reverse (-)
47665411 .. 47668295
2885 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0325000.1

Sequence Viewer

Length: 666 bp
ATGGAGACCCTTCTTGTTGTGGAGCATAAGAACCAGTACTACAGCCGGGTCAAGCCACATGGGCCGGGCCGGGTCGGACCCTCGCCGTCCAAGAGCTTCCGAGGGATCAATTGCCGGACTTTCCAATCCGGCACAGGTATACTACCAACCCCTCCTCCATTGAAGTCGTCTTCTACTACTACTACTAGTACTAGTACTCCTGTTTCGAAAAAACCATGCCCCCCTTCTGCTTATTCACCCAAGACACCTACACCTAGTGCGGATTCACAGTTTGATGCTAAGCCTAAATTTTACTCTGACAGTAAAGCTGTGAGTAGAAGCAATGTGAGAACCAACCCAATTCCTATCAATGCCCGAAACCTGAAGAAAGAGAAGCCTTTTAATGAGAGTTTTTCATTTTCGGAACTCTGGGCTGGTCCTGCTTACTCAAACTCTCCCCCACCTAGTTCTTTGCCAATACCCAAATTTTCAGTTCGGCCAAAGAGGACCGTGTCACTTGAATTGCCTAGCTCTGTTTCTGCTATTGAAATGCACCCAATTGCCAAGTCTGCACCCCCATCCCCGACCAGAGAGCATAGCTCTTCCAAAAGAGATCTCTTTCACAATGCTGACTCTGCCACTAGGACTCTGCGTCGCATCCTCAACCTGGATGTTGACGACGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2

Protein Analysis

221

Amino Acids

24.14

Weight (kDa)

10.0

Isoelectric Point (pI)

62.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNRC PF15365 137 - 156 2.6e-07 Proline-rich nuclear receptor coactivator motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017732)

Species Orthologous Gene IDs
malus_domestica MD08G1006200.v1.1 MD15G1005600.v1.1
prunus_persica Prupe.1G358200_v2.0.a1
pyrus_communis pycom15g00400
rosa_chinensis RchiOBHm_Chr6g0303811
rosa_laevigata RLG00000011042
rosa_multiflora Rmu_sc0012878.1_g000003
rosa_roxburghii Rroxscaffold_7G00164390
rosa_rugosa Rorug06G0325000
rosa_samantha Rh6AG439300 Rh6BG463700
rosa_wichuraiana Rw6G038030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 139
AciI CCGC 1 cut(s) 260
AclWI GGATC 1 cut(s) 113
AcoI YGGCCR 1 cut(s) 476
AcsI RAATTY 2 cut(s) 287, 464
AcuI CTGAAG 1 cut(s) 383
AdeI CACNNNGTG 1 cut(s) 257
AfaI GTAC 3 cut(s) 38, 190, 196
AfiI CCNNNNNNNGG 1 cut(s) 646
AgsI TTSAA 3 cut(s) 163, 500, 527
AhdI GACNNNNNGTC 1 cut(s) 630
AhlI ACTAGT 2 cut(s) 185, 191
AjnI CCWGG 1 cut(s) 645
AluBI AGCT 4 cut(s) 96, 308, 510, 579
AluI AGCT 4 cut(s) 96, 308, 510, 579
AlwI GGATC 1 cut(s) 113
AoxI GGCC 3 cut(s) 62, 67, 476
ApoI RAATTY 2 cut(s) 287, 464
AspS9I GGNCC 5 cut(s) 62, 67, 77, 416, 486
AsuC2I CCSGG 3 cut(s) 47, 66, 71
AsuHPI GGTGA 1 cut(s) 228
AsuII TTCGAA 1 cut(s) 206
AvaII GGWCC 3 cut(s) 77, 416, 486
BbsI GAAGAC 1 cut(s) 162
BccI CCATC 1 cut(s) 565
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 1 cut(s) 647
BcnI CCSGG 3 cut(s) 47, 66, 71
BcuI ACTAGT 2 cut(s) 185, 191
BfaI CTAG 6 cut(s) 186, 192, 255, 444, 507, 621
BfmI CTRYAG 1 cut(s) 40
BglI GCCNNNNNGGC 1 cut(s) 61
BglII AGATCT 1 cut(s) 592
BlpI GCTNAGC 1 cut(s) 279
BmcAI AGTACT 3 cut(s) 38, 190, 196
Bme1390I CCNGG 4 cut(s) 47, 66, 71, 647
Bme18I GGWCC 3 cut(s) 77, 416, 486
BmeRI GACNNNNNGTC 1 cut(s) 630
BmgT120I GGNCC 5 cut(s) 62, 67, 77, 416, 486
BmiI GGNNCC 1 cut(s) 79
BmrFI CCNGG 4 cut(s) 47, 66, 71, 647
BmsI GCATC 2 cut(s) 265, 645
BpiI GAAGAC 1 cut(s) 162
BplI GAGNNNNNCTC 2 cut(s) 563, 595
Bpu1102I GCTNAGC 1 cut(s) 279
Bpu14I TTCGAA 1 cut(s) 206
BpuMI CCSGG 3 cut(s) 47, 66, 71
BsaJI CCNNGG 1 cut(s) 100
BsaXI ACNNNNNCTCC 4 cut(s) 139, 169, 181, 211
Bsc4I CCNNNNNNNGG 1 cut(s) 646
Bse1I ACTGG 1 cut(s) 34
Bse3DI GCAATG 1 cut(s) 328
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 3 cut(s) 557, 636, 655
BseLI CCNNNNNNNGG 1 cut(s) 646
BseMI GCAATG 1 cut(s) 328
BseNI ACTGG 1 cut(s) 34
BseRI GAGGAG 1 cut(s) 144
BsgI GTGCAG 1 cut(s) 534
BshFI GGCC 3 cut(s) 64, 69, 478
BsiSI CCGG 5 cut(s) 46, 65, 70, 115, 129
BslI CCNNNNNNNGG 1 cut(s) 646
BsnI GGCC 3 cut(s) 64, 69, 478
Bsp119I TTCGAA 1 cut(s) 206
Bsp143I GATC 2 cut(s) 105, 592
Bsp1720I GCTNAGC 1 cut(s) 279
BspACI CCGC 1 cut(s) 260
BspANI GGCC 3 cut(s) 64, 69, 478
BspLI GGNNCC 1 cut(s) 79
BspPI GGATC 1 cut(s) 113
BspQI GCTCTTC 1 cut(s) 586
BspT104I TTCGAA 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 328
BsrI ACTGG 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 2 cut(s) 105, 592
BssNAI GTATAC 1 cut(s) 140
Bst1107I GTATAC 1 cut(s) 140
Bst2UI CCWGG 1 cut(s) 647
Bst4CI ACNGT 3 cut(s) 270, 302, 490
Bst6I CTCTTC 1 cut(s) 586
BstBI TTCGAA 1 cut(s) 206
BstDEI CTNAG 1 cut(s) 279
BstF5I GGATG 3 cut(s) 557, 636, 655
BstKTI GATC 2 cut(s) 108, 595
BstMBI GATC 2 cut(s) 105, 592
BstMWI GCNNNNNNNGC 4 cut(s) 61, 419, 548, 614
BstNI CCWGG 1 cut(s) 647
BstSCI CCNGG 4 cut(s) 45, 64, 69, 645
BstSFI CTRYAG 1 cut(s) 40
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 592
BstYI RGATCY 1 cut(s) 592
BstZ17I GTATAC 1 cut(s) 140
BsuRI GGCC 3 cut(s) 64, 69, 478
BtsCI GGATG 3 cut(s) 557, 636, 655
Cfr13I GGNCC 5 cut(s) 62, 67, 77, 416, 486
CseI GACGC 1 cut(s) 620
Csp6I GTAC 3 cut(s) 37, 189, 195
CviAII CATG 2 cut(s) 59, 216
CviQI GTAC 3 cut(s) 37, 189, 195
DdeI CTNAG 1 cut(s) 279
DpnI GATC 2 cut(s) 107, 594
DpnII GATC 2 cut(s) 105, 592
DraIII CACNNNGTG 1 cut(s) 257
DriI GACNNNNNGTC 1 cut(s) 630
EaeI YGGCCR 1 cut(s) 476
Eam1104I CTCTTC 1 cut(s) 586
Eam1105I GACNNNNNGTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 586
Eco47I GGWCC 3 cut(s) 77, 416, 486
Eco57I CTGAAG 1 cut(s) 383
EcoRII CCWGG 1 cut(s) 645
FaeI CATG 2 cut(s) 62, 219
FaiI YATR 5 cut(s) 27, 60, 140, 217, 576
FatI CATG 2 cut(s) 58, 215
FblI GTMKAC 1 cut(s) 139
FokI GGATG 3 cut(s) 544, 623, 662
FspBI CTAG 6 cut(s) 186, 192, 255, 444, 507, 621
HaeIII GGCC 3 cut(s) 64, 69, 478
HapII CCGG 5 cut(s) 46, 65, 70, 115, 129
HgaI GACGC 1 cut(s) 620
Hin1II CATG 2 cut(s) 62, 219
HincII GTYRAC 1 cut(s) 655
HindII GTYRAC 1 cut(s) 655
HinfI GANTC 3 cut(s) 263, 611, 625
HpaII CCGG 5 cut(s) 46, 65, 70, 115, 129
HphI GGTGA 1 cut(s) 228
Hpy166II GTNNAC 2 cut(s) 140, 655
Hpy188I TCNGA 4 cut(s) 77, 101, 298, 403
Hpy8I GTNNAC 2 cut(s) 140, 655
Hpy99I CGWCG 2 cut(s) 636, 662
HpyAV CCTTC 2 cut(s) 20, 234
HpyCH4III ACNGT 3 cut(s) 270, 302, 490
HpyCH4V TGCA 2 cut(s) 532, 551
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 419, 548, 614
HpyF3I CTNAG 1 cut(s) 279
Hsp92II CATG 2 cut(s) 62, 219
Kzo9I GATC 2 cut(s) 105, 592
LguI GCTCTTC 1 cut(s) 586
LmnI GCTCC 1 cut(s) 22
LweI GCATC 2 cut(s) 265, 645
MaeI CTAG 6 cut(s) 186, 192, 255, 444, 507, 621
MaeIII GTNAC 1 cut(s) 492
MalI GATC 2 cut(s) 107, 594
MboI GATC 2 cut(s) 105, 592
MboII GAAGA 3 cut(s) 162, 376, 573
MfeI CAATTG 2 cut(s) 109, 537
MflI RGATCY 1 cut(s) 592
MluCI AATT 6 cut(s) 109, 287, 339, 464, 500, 537
MlyI GAGTC 2 cut(s) 605, 619
MmeI TCCRAC 1 cut(s) 55
MnlI CCTC 6 cut(s) 91, 95, 162, 165, 477, 650
MseI TTAA 1 cut(s) 381
MspI CCGG 5 cut(s) 46, 65, 70, 115, 129
MspR9I CCNGG 4 cut(s) 47, 66, 71, 647
MunI CAATTG 2 cut(s) 109, 537
MvaI CCWGG 1 cut(s) 647
MwoI GCNNNNNNNGC 4 cut(s) 61, 419, 548, 614
NciI CCSGG 3 cut(s) 47, 66, 71
NdeII GATC 2 cut(s) 105, 592
NlaIII CATG 2 cut(s) 62, 219
NlaIV GGNNCC 1 cut(s) 79
NmuCI GTSAC 1 cut(s) 492
NspV TTCGAA 1 cut(s) 206
PciSI GCTCTTC 1 cut(s) 586
PfeI GAWTC 1 cut(s) 263
PflFI GACNNNGTC 1 cut(s) 490
PleI GAGTC 2 cut(s) 605, 619
PpsI GAGTC 2 cut(s) 605, 619
Psp6I CCWGG 1 cut(s) 645
PspGI CCWGG 1 cut(s) 645
PspN4I GGNNCC 1 cut(s) 79
PspPI GGNCC 5 cut(s) 62, 67, 77, 416, 486
PsrI GAACNNNNNNTAC 2 cut(s) 23, 55
PsuI RGATCY 1 cut(s) 592
PsyI GACNNNGTC 1 cut(s) 490
RsaI GTAC 3 cut(s) 38, 190, 196
RsaNI GTAC 3 cut(s) 37, 189, 195
SapI GCTCTTC 1 cut(s) 586
SaqAI TTAA 1 cut(s) 381
Sau3AI GATC 2 cut(s) 105, 592
Sau96I GGNCC 5 cut(s) 62, 67, 77, 416, 486
ScaI AGTACT 3 cut(s) 38, 190, 196
SchI GAGTC 2 cut(s) 605, 619
ScrFI CCNGG 4 cut(s) 47, 66, 71, 647
SfaNI GCATC 2 cut(s) 265, 645
SfcI CTRYAG 1 cut(s) 40
SfuI TTCGAA 1 cut(s) 206
SinI GGWCC 3 cut(s) 77, 416, 486
SpeI ACTAGT 2 cut(s) 185, 191
Sse9I AATT 6 cut(s) 109, 287, 339, 464, 500, 537
SsiI CCGC 1 cut(s) 260
SspMI CTAG 6 cut(s) 186, 192, 255, 444, 507, 621
StyD4I CCNGG 4 cut(s) 45, 64, 69, 645
TaaI ACNGT 3 cut(s) 270, 302, 490
TaqI TCGA 1 cut(s) 206
TasI AATT 6 cut(s) 109, 287, 339, 464, 500, 537
TatI WGTACW 3 cut(s) 36, 188, 194
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TseFI GTSAC 1 cut(s) 492
Tsp45I GTSAC 1 cut(s) 492
TspDTI ATGAA 1 cut(s) 384
Tth111I GACNNNGTC 1 cut(s) 490
VpaK11BI GGWCC 3 cut(s) 77, 416, 486
XapI RAATTY 2 cut(s) 287, 464
XmiI GTMKAC 1 cut(s) 139
XspI CTAG 6 cut(s) 186, 192, 255, 444, 507, 621
ZrmI AGTACT 3 cut(s) 38, 190, 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.