MD08G1033200.v1.1

Domain of unknown function (DUF4091)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008088
Physical Location & Seq
Reverse (-)
2357337 .. 2363029
5693 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1033200.v1.1.491

Sequence Viewer

Length: 1944 bp
ATGGATGATTCAGGGAATCCTCAGGACGTCGTTGTGCCACCAGTTGAAGGTGTTGCTGGAGGAGGGACGGCATATGGATGGACTGAGGGTGGCTTACAAGGTGCAAGTCCACTCAAGGGATCCATCGACCCTACAGAGGTTCCGACTGCTGATTTAGTGCATGTGTGGTGCATGCCAAGCACAGCAAATGTTGGACCACAAGAAACACCAAGGAATTTGGAGTCGGTTAATCTTCTGGCTGGTAGAAATGAAAGAGAGAGTACTCAAATTGCACTGCGTCCAAAAGTTTCTTGGGGTGATCCTGGAAATGCAGGGGTTGTGCAGGTCCAGTGTAGTGACTTGTGCTCCACATCTGGTGATCGGTTGGTTGTTGGACAATCAATTAACATGCGGCGTGTGGTTCCCATATTGGGTGTCCCAGATGCTCTTGCACCTCTTGATCTTCCAATTAGTCAAATAAACTTATTACCAGGAGAGACAACTGCAGTTTGGATTTCCATTGATGTTCCTAGTGCGCAACCCCCAGGACAGTATGAGGGAGAGATCATCATTACTGCTACAAAGGCAGATCCAGAGGCTCCAGCGCATTGCTTGGGCAAAGGCGAGAAGCATCAACTTTATAGGGATCTTCAAAGTTGTCTTCAAATGGTGGAGCCCATTGATGGGAAACCGGTGGATGAAGTGGTAGAAAGGGTGAAATCTGCTGCAACATCTTTGAGAAGAGCTCTTCTGTCGCCATTATTTTCTGAATTTTGTACATTAAATGGGCCAGTTGATATGATGGAGGAAGATGCTGTTTCGATCCTTTCAGTACGTGTGAAGATTAATATGACAGTTTGGGACTTCATTCTTCCTGCAACCCCATCACTTCCTGCTGTTTTTGGCATATCTGATACTGTAATTGAAGATCGTTTTGGTGTTGAACATGGGACTGATGAGTGGTATGAGGCACTGGATCAGCATTTTAAGTGGCTTCTTCAATACAAAATCAGTCCATACTTTTGCAGATGGGGTGATAGTATGCGTGTCTTGACGTATACCTGCCCTTGGCCAGCTGATCATCCAAAATCAGATGAATACTTTTCGGATCCACGGTTGGCAGCCTATGCTTTGCCATATAGTCGATCTGTCTCTGGTGGTGATGCAGCAAAGGATTACATGCAGAAACAAATTGATATATTGAGGACAAAGTCTCACTGGAAGAAAGCATACTTTTACTTGTGGGATGAGCCACTGAATCTGGAGCAATATGAGTCCCTCCGCAGCATAGCCAGTGAGATCCATGCGTATGCGCCTGATGCTCGTGTTTTAACTACTTACTACTGTGGGCCTAGCAATGCACCTACTGCGTTTGACGGTTTTGTGAAAGTTCCAAAATTCCTGCGCCCACATACTCAAATTTATTGTACAAGTGAATGGGTGCTTGGGAATCGAGAGGATTTGGTCAAAGATATTGTTTCCGAAATACAAACAGAAAATGGCGAGGAATGGTGGACTTATGTGTGCTTGGGACCGTCTGACCCCCATCCCAATTGGCACCTTGGGATGCGAGGTACACAACACCGTGCTGTAATGTGGCGTGTATGGAAAGAAGGTGGAACGGGTTTCCTATATTGGGGTGCCAATTGCTATGAGAAGGCAAATGTTCCAAGCGCAGAGATAAGATTCAGGCGTGGCCTTCCCCCCGGAGATGGAGTTTTGTTCTACCCTGGCCAGGTGTTCTCATCATCAAGTCAACCAGTTGCTTCGGTCAGACTCGAGCGCATTCTCAGTGGCTTGCAGGACATTGAGTACCTCAGACTCTACTCTTCAAGATACGGTCGAGATGAAGGACTGGCTCTCCTTGAAAAGACGGGTTTGTACCTCGGTCCTGAGCGTTACACGCACGATCACACGCCCATTGATGCAATGCGGGGTGAGATCTACAATGCGTGTCGCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4091

Protein Analysis

648

Amino Acids

71.99

Weight (kDa)

5.07

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH123_cat PF13320 382 - 601 5.1e-15 Glycoside hydrolase 123, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 30
Acc16I TGCGCA 1 cut(s) 516
Acc36I ACCTGC 2 cut(s) 313, 1049
AccB1I GGYRCC 2 cut(s) 1536, 1619
AccI GTMKAC 1 cut(s) 1037
AciI CCGC 3 cut(s) 391, 1261, 1912
AcoI YGGCCR 2 cut(s) 1049, 1711
AcsI RAATTY 4 cut(s) 214, 749, 1376, 1398
AcyI GRCGYC 1 cut(s) 27
AfaI GTAC 7 cut(s) 262, 757, 813, 1408, 1555, 1793, 1862
AflIII ACRYGT 1 cut(s) 814
AgeI ACCGGT 1 cut(s) 670
AgsI TTSAA 8 cut(s) 47, 632, 644, 905, 923, 980, 1812, 1847
AjnI CCWGG 5 cut(s) 301, 469, 523, 1708, 1713
AjuI GAANNNNNNNTTGG 4 cut(s) 897, 929, 1407, 1439
AluBI AGCT 2 cut(s) 725, 1055
AluI AGCT 2 cut(s) 725, 1055
Alw21I GWGCWC 2 cut(s) 347, 727
Alw26I GTCTC 3 cut(s) 470, 1135, 1197
Ama87I CYCGRG 1 cut(s) 1757
AoxI GGCC 5 cut(s) 767, 1049, 1328, 1675, 1711
ApeKI GCWGC 4 cut(s) 704, 1100, 1145, 1263
ApoI RAATTY 4 cut(s) 214, 749, 1376, 1398
AseI ATTAAT 1 cut(s) 825
AsiGI ACCGGT 1 cut(s) 670
AspLEI GCGC 6 cut(s) 517, 586, 1294, 1386, 1655, 1764
AspS9I GGNCC 6 cut(s) 194, 325, 767, 1328, 1511, 1868
AsuC2I CCSGG 1 cut(s) 1686
AsuHPI GGTGA 6 cut(s) 308, 368, 706, 1025, 1151, 1928
AvaI CYCGRG 1 cut(s) 1757
AvaII GGWCC 4 cut(s) 194, 325, 1511, 1868
AxyI CCTNAGG 1 cut(s) 21
BalI TGGCCA 2 cut(s) 1051, 1713
BamHI GGATCC 2 cut(s) 119, 1087
BanI GGYRCC 2 cut(s) 1536, 1619
BanII GRGCYC 2 cut(s) 657, 727
BarI GAAGNNNNNNTAC 2 cut(s) 1193, 1225
BauI CACGAG 2 cut(s) 1302, 1939
BbsI GAAGAC 1 cut(s) 632
Bbv12I GWGCWC 2 cut(s) 347, 727
BbvI GCAGC 4 cut(s) 691, 1112, 1157, 1275
BccI CCATC 8 cut(s) 72, 131, 656, 775, 871, 1002, 1533, 1685
BceAI ACGGC 1 cut(s) 84
BciT130I CCWGG 5 cut(s) 303, 471, 525, 1710, 1715
BclI TGATCA 1 cut(s) 1057
BcnI CCSGG 1 cut(s) 1686
BcoDI GTCTC 3 cut(s) 470, 1135, 1197
BfaI CTAG 2 cut(s) 510, 1332
BfmI CTRYAG 2 cut(s) 132, 483
BfuAI ACCTGC 2 cut(s) 313, 1049
BglII AGATCT 1 cut(s) 1920
BisI GCNGC 5 cut(s) 392, 705, 1101, 1146, 1264
BlsI GCNGC 5 cut(s) 393, 706, 1102, 1147, 1265
BmcAI AGTACT 1 cut(s) 262
Bme1390I CCNGG 6 cut(s) 303, 471, 525, 1686, 1710, 1715
Bme18I GGWCC 4 cut(s) 194, 325, 1511, 1868
BmeT110I CYCGRG 1 cut(s) 1757
BmgT120I GGNCC 6 cut(s) 194, 325, 767, 1328, 1511, 1868
BmiI GGNNCC 9 cut(s) 121, 141, 402, 579, 654, 1089, 1512, 1538, 1621
BmrFI CCNGG 6 cut(s) 303, 471, 525, 1686, 1710, 1715
BmsI GCATC 7 cut(s) 412, 619, 781, 1132, 1288, 1536, 1894
BpiI GAAGAC 1 cut(s) 632
BplI GAGNNNNNCTC 2 cut(s) 709, 741
BpmI CTGGAG 3 cut(s) 78, 564, 1262
Bpu10I CCTNAGC 1 cut(s) 1872
BpuEI CTTGAG 1 cut(s) 98
BpuMI CCSGG 1 cut(s) 1686
BsaAI YACGTR 1 cut(s) 815
BsaHI GRCGYC 1 cut(s) 27
BsaJI CCNNGG 8 cut(s) 209, 523, 1046, 1091, 1540, 1684, 1708, 1864
BsaWI WCCGGW 1 cut(s) 670
BsaXI ACNNNNNCTCC 6 cut(s) 329, 359, 1680, 1710, 1824, 1854
Bse118I RCCGGY 1 cut(s) 670
Bse1I ACTGG 8 cut(s) 41, 328, 770, 957, 1202, 1272, 1739, 1839
Bse21I CCTNAGG 1 cut(s) 21
Bse3DI GCAATG 3 cut(s) 586, 1342, 1914
BseBI CCWGG 5 cut(s) 303, 471, 525, 1710, 1715
BseDI CCNNGG 8 cut(s) 209, 523, 1046, 1091, 1540, 1684, 1708, 1864
BseGI GGATG 7 cut(s) 10, 83, 682, 1060, 1231, 1525, 1551
BseMI GCAATG 3 cut(s) 586, 1342, 1914
BseMII CTCAG 5 cut(s) 35, 75, 1783, 1810, 1863
BseNI ACTGG 8 cut(s) 41, 328, 770, 957, 1202, 1272, 1739, 1839
BseRI GAGGAG 1 cut(s) 75
BseXI GCAGC 4 cut(s) 691, 1112, 1157, 1275
BsgI GTGCAG 1 cut(s) 341
Bsh1285I CGRYCG 1 cut(s) 1822
BshFI GGCC 5 cut(s) 769, 1051, 1330, 1677, 1713
BshNI GGYRCC 2 cut(s) 1536, 1619
BshTI ACCGGT 1 cut(s) 670
BsiEI CGRYCG 1 cut(s) 1822
BsiHKAI GWGCWC 2 cut(s) 347, 727
BsiHKCI CYCGRG 1 cut(s) 1757
BsiSI CCGG 2 cut(s) 671, 1686
BslFI GGGAC 6 cut(s) 79, 401, 854, 943, 1240, 1524
BsmAI GTCTC 3 cut(s) 470, 1135, 1197
BsmFI GGGAC 6 cut(s) 79, 401, 854, 943, 1240, 1524
BsmI GAATGC 1 cut(s) 1764
BsnI GGCC 5 cut(s) 769, 1051, 1330, 1677, 1713
BsoBI CYCGRG 1 cut(s) 1757
Bsp1286I GDGCHC 3 cut(s) 347, 657, 727
Bsp1407I TGTACA 2 cut(s) 755, 1406
BspACI CCGC 3 cut(s) 391, 1261, 1912
BspANI GGCC 5 cut(s) 769, 1051, 1330, 1677, 1713
BspCNI CTCAG 5 cut(s) 34, 76, 1782, 1809, 1864
BspLI GGNNCC 9 cut(s) 121, 141, 402, 579, 654, 1089, 1512, 1538, 1621
BspMAI CTGCAG 1 cut(s) 487
BspMI ACCTGC 2 cut(s) 313, 1049
BspQI GCTCTTC 2 cut(s) 715, 732
BspT107I GGYRCC 2 cut(s) 1536, 1619
BsrDI GCAATG 3 cut(s) 586, 1342, 1914
BsrFI RCCGGY 1 cut(s) 670
BsrGI TGTACA 2 cut(s) 755, 1406
BsrI ACTGG 8 cut(s) 41, 328, 770, 957, 1202, 1272, 1739, 1839
BssAI RCCGGY 1 cut(s) 670
BssECI CCNNGG 8 cut(s) 209, 523, 1046, 1091, 1540, 1684, 1708, 1864
BssNAI GTATAC 1 cut(s) 1038
BssNI GRCGYC 1 cut(s) 27
BssSI CACGAG 2 cut(s) 1302, 1939
BssT1I CCWWGG 3 cut(s) 209, 1046, 1540
Bst1107I GTATAC 1 cut(s) 1038
Bst2BI CACGAG 2 cut(s) 1302, 1939
Bst2UI CCWGG 5 cut(s) 303, 471, 525, 1710, 1715
Bst4CI ACNGT 9 cut(s) 531, 835, 898, 1095, 1325, 1358, 1515, 1565, 1820
Bst6I CTCTTC 3 cut(s) 715, 732, 1813
BstACI GRCGYC 1 cut(s) 27
BstAPI GCANNNNNTGC 2 cut(s) 1106, 1346
BstAUI TGTACA 2 cut(s) 755, 1406
BstBAI YACGTR 1 cut(s) 815
BstC8I GCNNGC 3 cut(s) 173, 1053, 1778
BstDEI CTNAG 5 cut(s) 21, 84, 1769, 1796, 1872
BstDSI CCRYGG 1 cut(s) 1091
BstF5I GGATG 7 cut(s) 10, 83, 682, 1060, 1231, 1525, 1551
BstHHI GCGC 6 cut(s) 517, 586, 1294, 1386, 1655, 1764
BstMAI GTCTC 3 cut(s) 470, 1135, 1197
BstMCI CGRYCG 1 cut(s) 1822
BstMWI GCNNNNNNNGC 6 cut(s) 177, 563, 1106, 1298, 1346, 1882
BstNI CCWGG 5 cut(s) 303, 471, 525, 1710, 1715
BstNSI RCATGY 4 cut(s) 164, 175, 391, 1162
BstSCI CCNGG 6 cut(s) 301, 469, 523, 1684, 1708, 1713
BstSFI CTRYAG 2 cut(s) 132, 483
BstV1I GCAGC 4 cut(s) 691, 1112, 1157, 1275
BstV2I GAAGAC 1 cut(s) 632
BstX2I RGATCY 6 cut(s) 119, 568, 625, 1087, 1278, 1920
BstYI RGATCY 6 cut(s) 119, 568, 625, 1087, 1278, 1920
BstZ17I GTATAC 1 cut(s) 1038
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 5 cut(s) 769, 1051, 1330, 1677, 1713
BtgI CCRYGG 1 cut(s) 1091
BtsCI GGATG 7 cut(s) 10, 83, 682, 1060, 1231, 1525, 1551
BtsI GCAGTG 1 cut(s) 272
BtsIMutI CAGTG 7 cut(s) 272, 335, 950, 1195, 1232, 1279, 1777
BveI ACCTGC 2 cut(s) 313, 1049
Cac8I GCNNGC 3 cut(s) 173, 1053, 1778
CfoI GCGC 6 cut(s) 517, 586, 1294, 1386, 1655, 1764
Cfr10I RCCGGY 1 cut(s) 670
Cfr13I GGNCC 6 cut(s) 194, 325, 767, 1328, 1511, 1868
CseI GACGC 1 cut(s) 266
Csp6I GTAC 7 cut(s) 261, 756, 812, 1407, 1554, 1792, 1861
CspAI ACCGGT 1 cut(s) 670
CviAII CATG 6 cut(s) 161, 172, 388, 926, 1159, 1283
CviQI GTAC 7 cut(s) 261, 756, 812, 1407, 1554, 1792, 1861
DdeI CTNAG 5 cut(s) 21, 84, 1769, 1796, 1872
EaeI YGGCCR 2 cut(s) 1049, 1711
Eam1104I CTCTTC 3 cut(s) 715, 732, 1813
EarI CTCTTC 3 cut(s) 715, 732, 1813
Ecl136II GAGCTC 1 cut(s) 725
Eco130I CCWWGG 3 cut(s) 209, 1046, 1540
Eco24I GRGCYC 2 cut(s) 657, 727
Eco47I GGWCC 4 cut(s) 194, 325, 1511, 1868
Eco53kI GAGCTC 1 cut(s) 725
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 1757
EcoICRI GAGCTC 1 cut(s) 725
EcoRII CCWGG 5 cut(s) 301, 469, 523, 1708, 1713
EcoT14I CCWWGG 3 cut(s) 209, 1046, 1540
EcoT38I GRGCYC 2 cut(s) 657, 727
ErhI CCWWGG 3 cut(s) 209, 1046, 1540
FaeI CATG 6 cut(s) 164, 175, 391, 929, 1162, 1286
FaqI GGGAC 6 cut(s) 79, 401, 854, 943, 1240, 1524
FatI CATG 6 cut(s) 160, 171, 387, 925, 1158, 1282
FauI CCCGC 1 cut(s) 1905
FauNDI CATATG 1 cut(s) 73
FbaI TGATCA 1 cut(s) 1057
FblI GTMKAC 1 cut(s) 1037
Fnu4HI GCNGC 5 cut(s) 392, 705, 1101, 1146, 1264
FokI GGATG 7 cut(s) 17, 90, 689, 1047, 1238, 1512, 1558
FriOI GRGCYC 2 cut(s) 657, 727
Fsp4HI GCNGC 5 cut(s) 392, 705, 1101, 1146, 1264
FspBI CTAG 2 cut(s) 510, 1332
FspI TGCGCA 1 cut(s) 516
GlaI GCGC 6 cut(s) 516, 585, 1293, 1385, 1654, 1763
GluI GCNGC 5 cut(s) 392, 705, 1101, 1146, 1264
GsuI CTGGAG 3 cut(s) 78, 564, 1262
HaeIII GGCC 5 cut(s) 769, 1051, 1330, 1677, 1713
HapII CCGG 2 cut(s) 671, 1686
HgaI GACGC 1 cut(s) 266
HhaI GCGC 6 cut(s) 517, 586, 1294, 1386, 1655, 1764
Hin1I GRCGYC 1 cut(s) 27
Hin1II CATG 6 cut(s) 164, 175, 391, 929, 1162, 1286
Hin6I GCGC 6 cut(s) 515, 584, 1292, 1384, 1653, 1762
HinP1I GCGC 6 cut(s) 515, 584, 1292, 1384, 1653, 1762
HincII GTYRAC 1 cut(s) 1736
HindII GTYRAC 1 cut(s) 1736
HinfI GANTC 9 cut(s) 8, 16, 221, 1237, 1253, 1429, 1665, 1755, 1800
HpaII CCGG 2 cut(s) 671, 1686
HphI GGTGA 6 cut(s) 308, 368, 706, 1025, 1151, 1928
Hpy166II GTNNAC 5 cut(s) 110, 1038, 1494, 1556, 1736
Hpy188I TCNGA 9 cut(s) 144, 748, 892, 1072, 1087, 1462, 1519, 1754, 1799
Hpy8I GTNNAC 5 cut(s) 110, 1038, 1494, 1556, 1736
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 5 cut(s) 41, 1586, 1630, 1688, 1823
HpyCH4III ACNGT 9 cut(s) 531, 835, 898, 1095, 1325, 1358, 1515, 1565, 1820
HpyCH4IV ACGT 3 cut(s) 27, 814, 1034
HpyF10VI GCNNNNNNNGC 6 cut(s) 177, 563, 1106, 1298, 1346, 1882
HpyF3I CTNAG 5 cut(s) 21, 84, 1769, 1796, 1872
HpySE526I ACGT 3 cut(s) 27, 814, 1034
Hsp92I GRCGYC 1 cut(s) 27
Hsp92II CATG 6 cut(s) 164, 175, 391, 929, 1162, 1286
HspAI GCGC 6 cut(s) 515, 584, 1292, 1384, 1653, 1762
Ksp22I TGATCA 1 cut(s) 1057
LguI GCTCTTC 2 cut(s) 715, 732
LmnI GCTCC 4 cut(s) 350, 583, 652, 1243
Lsp1109I GCAGC 4 cut(s) 691, 1112, 1157, 1275
LweI GCATC 7 cut(s) 412, 619, 781, 1132, 1288, 1536, 1894
MaeI CTAG 2 cut(s) 510, 1332
MaeII ACGT 3 cut(s) 27, 814, 1034
MaeIII GTNAC 2 cut(s) 335, 1877
MfeI CAATTG 2 cut(s) 1531, 1624
MflI RGATCY 6 cut(s) 119, 568, 625, 1087, 1278, 1920
MhlI GDGCHC 3 cut(s) 347, 657, 727
MlsI TGGCCA 2 cut(s) 1051, 1713
MluNI TGGCCA 2 cut(s) 1051, 1713
MlyI GAGTC 4 cut(s) 230, 1262, 1749, 1794
MmeI TCCRAC 3 cut(s) 167, 172, 352
Mox20I TGGCCA 2 cut(s) 1051, 1713
MscI TGGCCA 2 cut(s) 1051, 1713
MseI TTAA 6 cut(s) 228, 384, 761, 825, 966, 1310
MslI CAYNNNNRTG 2 cut(s) 76, 1287
Msp20I TGGCCA 2 cut(s) 1051, 1713
MspA1I CMGCKG 1 cut(s) 1055
MspI CCGG 2 cut(s) 671, 1686
MspR9I CCNGG 6 cut(s) 303, 471, 525, 1686, 1710, 1715
MunI CAATTG 2 cut(s) 1531, 1624
Mva1269I GAATGC 1 cut(s) 1764
MvaI CCWGG 5 cut(s) 303, 471, 525, 1710, 1715
MwoI GCNNNNNNNGC 6 cut(s) 177, 563, 1106, 1298, 1346, 1882
NciI CCSGG 1 cut(s) 1686
NdeI CATATG 1 cut(s) 73
NlaIII CATG 6 cut(s) 164, 175, 391, 929, 1162, 1286
NlaIV GGNNCC 9 cut(s) 121, 141, 402, 579, 654, 1089, 1512, 1538, 1621
NmuCI GTSAC 1 cut(s) 335
NsbI TGCGCA 1 cut(s) 516
NspI RCATGY 4 cut(s) 164, 175, 391, 1162
PaeI GCATGC 1 cut(s) 175
PaeR7I CTCGAG 1 cut(s) 1757
PciSI GCTCTTC 2 cut(s) 715, 732
PctI GAATGC 1 cut(s) 1764
PfeI GAWTC 5 cut(s) 8, 16, 1237, 1429, 1665
PflFI GACNNNGTC 1 cut(s) 1189
PfoI TCCNGGA 1 cut(s) 301
PinAI ACCGGT 1 cut(s) 670
PkrI GCNGC 5 cut(s) 393, 706, 1102, 1147, 1265
PleI GAGTC 4 cut(s) 229, 1261, 1749, 1794
PpsI GAGTC 4 cut(s) 229, 1261, 1749, 1794
Ppu21I YACGTR 1 cut(s) 815
PshBI ATTAAT 1 cut(s) 825
Psp124BI GAGCTC 1 cut(s) 727
Psp6I CCWGG 5 cut(s) 301, 469, 523, 1708, 1713
PspGI CCWGG 5 cut(s) 301, 469, 523, 1708, 1713
PspN4I GGNNCC 9 cut(s) 121, 141, 402, 579, 654, 1089, 1512, 1538, 1621
PspPI GGNCC 6 cut(s) 194, 325, 767, 1328, 1511, 1868
PspXI VCTCGAGB 1 cut(s) 1757
PstI CTGCAG 1 cut(s) 487
PsuI RGATCY 6 cut(s) 119, 568, 625, 1087, 1278, 1920
PsyI GACNNNGTC 1 cut(s) 1189
PvuII CAGCTG 1 cut(s) 1055
RsaI GTAC 7 cut(s) 262, 757, 813, 1408, 1555, 1793, 1862
RsaNI GTAC 7 cut(s) 261, 756, 812, 1407, 1554, 1792, 1861
RseI CAYNNNNRTG 2 cut(s) 76, 1287
SacI GAGCTC 1 cut(s) 727
SapI GCTCTTC 2 cut(s) 715, 732
SaqAI TTAA 6 cut(s) 228, 384, 761, 825, 966, 1310
SatI GCNGC 5 cut(s) 392, 705, 1101, 1146, 1264
Sau96I GGNCC 6 cut(s) 194, 325, 767, 1328, 1511, 1868
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 4 cut(s) 230, 1262, 1749, 1794
ScrFI CCNGG 6 cut(s) 303, 471, 525, 1686, 1710, 1715
SduI GDGCHC 3 cut(s) 347, 657, 727
SfaNI GCATC 7 cut(s) 412, 619, 781, 1132, 1288, 1536, 1894
SfcI CTRYAG 2 cut(s) 132, 483
Sfr274I CTCGAG 1 cut(s) 1757
SinI GGWCC 4 cut(s) 194, 325, 1511, 1868
SlaI CTCGAG 1 cut(s) 1757
SmiMI CAYNNNNRTG 2 cut(s) 76, 1287
SmlI CTYRAG 2 cut(s) 113, 1757
SmoI CTYRAG 2 cut(s) 113, 1757
SphI GCATGC 1 cut(s) 175
SsiI CCGC 3 cut(s) 391, 1261, 1912
SspMI CTAG 2 cut(s) 510, 1332
SstI GAGCTC 1 cut(s) 727
StyD4I CCNGG 6 cut(s) 301, 469, 523, 1684, 1708, 1713
StyI CCWWGG 3 cut(s) 209, 1046, 1540
TaaI ACNGT 9 cut(s) 531, 835, 898, 1095, 1325, 1358, 1515, 1565, 1820
TaiI ACGT 3 cut(s) 30, 817, 1037
TaqI TCGA 6 cut(s) 126, 800, 1123, 1432, 1758, 1822
TaqII GACCGA 2 cut(s) 1738, 1856
TatI WGTACW 3 cut(s) 260, 755, 1406
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 5 cut(s) 8, 16, 1237, 1429, 1665
Tru1I TTAA 6 cut(s) 228, 384, 761, 825, 966, 1310
Tru9I TTAA 6 cut(s) 228, 384, 761, 825, 966, 1310
TscAI CASTG 7 cut(s) 279, 335, 957, 1202, 1239, 1279, 1777
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 4 cut(s) 704, 1100, 1145, 1263
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 5 cut(s) 264, 693, 835, 1089, 1842
TspRI CASTG 7 cut(s) 279, 335, 957, 1202, 1239, 1279, 1777
Tth111I GACNNNGTC 1 cut(s) 1189
VpaK11BI GGWCC 4 cut(s) 194, 325, 1511, 1868
VspI ATTAAT 1 cut(s) 825
XapI RAATTY 4 cut(s) 214, 749, 1376, 1398
XceI RCATGY 4 cut(s) 164, 175, 391, 1162
XcmI CCANNNNNNNNNTGG 1 cut(s) 288
XhoI CTCGAG 1 cut(s) 1757
XmiI GTMKAC 1 cut(s) 1037
XspI CTAG 2 cut(s) 510, 1332
ZraI GACGTC 1 cut(s) 28
ZrmI AGTACT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.