Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006866

Physical Location & Seq

Genomic Coordinates Reverse (-)

2372360 .. 2376419

Gene Structure 4060 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1033500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2412 bp

ATGGACAGAGACGATGACTTTGAGCTACTGTCACTGGAGGCTGCTCCTCCGGTCCGCGAAACGAAGCTGAAGCGCTTAAAGAAAGCAATTAGGGTTTCCGAAGAGCCTCCCGTTGACGAATCCGAGAGTGGCCCAGTGAATCCCTCTGAATTCGAAGCTCTAAGCCTGGAGGAATCGAATGAGCCGTCGAGGTTAGGGTTGGGATCAGAAGGTTTGGGCGGCGAAGGGGATTCTGATTCCGGGTTTCACGGTTTGGGCGGTGGTGAAGGTGAAATGAGTTCTGGTTCCGACGGGTTCGGCGGTGGAGGTGAAATCGATTCCGGGTTTGATGGTTTGGGCGGTGAAAGTGAAATGAAGTCGAATTTTGATGGTTTGGGCGGCGGTGAAGACGGCAATGGAGCTAAGAGGGTTTTGGATTTTGATTCTGTGGAAGAGGAGTTTGGTGAAGAAGGCAAAGATCGAAACTGGGAGATGCCGGAAGAAAGTGATGATGTGAGGGTAGAAGAGACGGATAAGAAACGGTGTAGTTCTGATGGCTTTGAGGAAGAAGAGAACATGAAGAAGAGCAAGAGGGCCAAGAGCGAAGCTGCAGCTGTCAAGGGCAAGAGAACGACTGAAAAAGAAAGAAAAGAGCATCTGAAGCAACTGCATGCTGAATCTCAGAGACTTTTGCGAGAAACCAGAGATGCAGCATTTAAACCCATACCGCTTGTTCAGAAGCCGATATCTTCAGTTCTGGAGAAGATTCGGAAAAGGAAACTGGAGGTTTCACAAAAATGTAGTAGTACAGGAGATTATAAGGTACTGAAAGCGGCAACTGAGGAGCCAATTGCATCTCAGACTGAAAAGGGGTATAGTGCAAATGAGGTGTGCAAAGATGGGTCTAATGATAAGGAGGAGCATTCAAACGGTGAAAATGCTCCTTCCGGGATGGATGTTGACGAGGAACCCAAACAAACATTTCGAGCACCTATTGATGATACTCAGGACCTGTTTTCTGATTCCCAGACTACCGACTCCAAGGATGATCTGCCAAATGATGATCCTAAAAGTCCGCTGGAAGAGGTTTTTGCACCGTCCATACCTGCCATGAACTTAAAACTTGACTCTGCTCCTCCTGATGATGTTTCTTCTGACGAGGAGGACTACAATGACAAAGAAAATATTGCTCCTTGTTCTCCTGAAGTGGCTGACGTGCCTTCATCTCCAATCGGTGATCCTGTAAAAGCTTTTGTGGATGATGAAGCTGAGGAAGAAGACGATAGTGATCATGACCTACATCGGTTCGAAGATACTGACGAAGATGAGGATGATGGAGACACTGCGGAGCTTAATGATATGATAGCAACTGGATATGAAGAAAGGCCAGTTGATGATGAAAGGCGGAATGAACTCCATCAAAAGTGGCTCGAGCAACAGGATGCCTCTGGAACAGAAAAACTTATGCAGAAGCTGAAGTTTGGTTCTAAAGGAAAAAAAACAGCATTGGTTGAAGAGAAAGATGCAGAGGGACAGGAAGATGAAGAGTTTGGTGATGAAAATGCAGAAGATGAAGAGCTTGGTGATGACGATGCAGCAGAAGATTCAGCACCAGCAAATGCTGTGCGAATGAACTTACGAGAAATAAAGCAAATGATGCCTCTACTGTATACTGATAACGATGATGTATACTTGTCATCTGACGATGATGAGACAGAAAAGAGACTATCTAAACAGTGCCTGTCTGAGAAAGCTGAAATGCAGGCTACTTTCTTGTCACCAGCAGAGGATGAGAGTTCAAGAGAAGTTTTTGGTCATATAAAGAAACTGAATATTGCGCCTGATACTAAGAAGGCCAAAGCCCCTTCCTTCCCCAATATCCTGCTTGCAGGAGGAGACAGGAGCATATCATCAAAGTCATCCTTCTTAGGTCGGGGGTCAAGTCATTCTCTGCCCTCATCCAATAAGCATGGACTGAGCACAGTTCGTTCTTTCATCTTAGTACGAGGTGACAGCAATAGTAGGAGCTCAATGTTGGAGGATTCTTCAGACACGATACAGAAGGAGATTCTACCGAAAAAGACTACTACAGCCAAGCTTAGCAAGTCCAAGGCAAAGTTTGGTATTCAGAAATCGTCACCTGACACGGTATCAACTGAAGCTGTTGCGAATACAGAAACACTGGTTGAAGCTGGTGCACAGATAGACAGATCAGCTGGAGCGGGTGCGCAGGCGGAGGCATCAGCTGAAGCTGGTATGGGGACAAACACATCAGCTGGATCTTCGTTACTCACGGTATTAAGGCAGCCTTCCTTGTCCTCAAAGCGATGCACCGGGGAGATTACTGTTGACCAAATCGAATCCGTCTTAGCTTCATTGGAAGACGGAAGGAAATCGAAGCGGGCGAACGGACATGTATACAGAAGAACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

804

Amino Acids

87.37

Weight (kDa)

4.5

Isoelectric Point (pI)

56.38

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006866)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75150 AT1G75150 AT1G75150
fragaria_vesca	FvH4_2g39970 FvH4_2g39970 FvH4_2g39970 FvH4_2g39970 FvH4_2g39970 FvH4_2g39970
malus_domestica	MD08G1033500.v1.1 MD15G1030200.v1.1
prunus_persica	Prupe.1G381400_v2.0.a1 Prupe.1G381400_v2.0.a1
pyrus_communis	pycom08g02670 pycom08g02680 pycom15g02650
rosa_chinensis	RchiOBHm_Chr6g0300781
rosa_laevigata	RLG00000011302
rosa_multiflora	Rmu_sc0011226.1_g000002 Rmu_sc0028577.1_g000001
rosa_roxburghii	Rroxscaffold_7G00167420
rosa_rugosa	Rorug06G0299200
rosa_samantha	Rh6AG412000 Rh6BG419200 Rh6CG426700 Rh6DG412000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	798
Acc16I	TGCGCA	1 cut(s)	2208
Acc36I	ACCTGC	1 cut(s)	1093
AccBSI	CCGCTC	1 cut(s)	2201
AccI	GTMKAC	3 cut(s)	1649, 1668, 2397
AccII	CGCG	1 cut(s)	57
AclWI	GGATC	4 cut(s)	211, 1037, 1211, 2266
AcsI	RAATTY	2 cut(s)	149, 361
AcuI	CTGAAG	8 cut(s)	89, 659, 714, 1203, 1475, 2010, 2157, 2247
AfaI	GTAC	3 cut(s)	787, 804, 1983
AfeI	AGCGCT	1 cut(s)	74
AflIII	ACRYGT	1 cut(s)	2392
AgsI	TTSAA	4 cut(s)	906, 1493, 1779, 2168
AjiI	CACGTC	1 cut(s)	1195
AjnI	CCWGG	1 cut(s)	165
AjuI	GAANNNNNNNTTGG	2 cut(s)	423, 455
Alw21I	GWGCWC	4 cut(s)	970, 1961, 2009, 2179
Alw26I	GTCTC	7 cut(s)	3, 500, 658, 1311, 1685, 1696, 1869
Alw44I	GTGCAC	1 cut(s)	2175
AlwI	GGATC	4 cut(s)	211, 1037, 1211, 2266
AlwNI	CAGNNNCTG	3 cut(s)	991, 1453, 1720
Ama87I	CYCGRG	1 cut(s)	1409
Aor51HI	AGCGCT	1 cut(s)	74
AoxI	GGCC	4 cut(s)	130, 573, 1364, 1833
ApaLI	GTGCAC	1 cut(s)	2175
ApeKI	GCWGC	6 cut(s)	41, 587, 590, 689, 1574, 2284
ApoI	RAATTY	2 cut(s)	149, 361
ArsI	GACNNNNNNTTYG	3 cut(s)	34, 170, 202
AspLEI	GCGC	3 cut(s)	75, 1819, 2209
AspS9I	GGNCC	4 cut(s)	52, 131, 573, 988
AsuC2I	CCSGG	4 cut(s)	241, 322, 928, 2314
AsuII	TTCGAA	2 cut(s)	153, 1287
AvaI	CYCGRG	1 cut(s)	1409
AvaII	GGWCC	2 cut(s)	52, 988
BaeGI	GKGCMC	1 cut(s)	2179
BanII	GRGCYC	1 cut(s)	2009
BbsI	GAAGAC	3 cut(s)	393, 1263, 2367
Bbv12I	GWGCWC	4 cut(s)	970, 1961, 2009, 2179
BbvCI	CCTCAGC	1 cut(s)	1248
BbvI	GCAGC	6 cut(s)	28, 574, 602, 701, 1586, 2296
BccI	CCATC	7 cut(s)	323, 362, 527, 872, 925, 1307, 1404
BceAI	ACGGC	2 cut(s)	169, 406
BciT130I	CCWGG	1 cut(s)	167
BclI	TGATCA	1 cut(s)	1267
BcnI	CCSGG	4 cut(s)	241, 322, 928, 2314
BcoDI	GTCTC	7 cut(s)	3, 500, 658, 1311, 1685, 1696, 1869
BfaI	CTAG	1 cut(s)	2410
BfmI	CTRYAG	2 cut(s)	588, 2067
BfoI	RGCGCY	1 cut(s)	76
BfuAI	ACCTGC	1 cut(s)	1093
BisI	GCNGC	9 cut(s)	42, 220, 379, 588, 591, 690, 813, 1575, 2285
BlpI	GCTNAGC	1 cut(s)	2078
BlsI	GCNGC	9 cut(s)	43, 221, 380, 589, 592, 691, 814, 1576, 2286
Bme1390I	CCNGG	5 cut(s)	167, 241, 322, 928, 2314
Bme18I	GGWCC	2 cut(s)	52, 988
BmeT110I	CYCGRG	1 cut(s)	1409
BmgBI	CACGTC	1 cut(s)	1195
BmgT120I	GGNCC	4 cut(s)	52, 131, 573, 988
BmiI	GGNNCC	3 cut(s)	286, 825, 948
BmrFI	CCNGG	5 cut(s)	167, 241, 322, 928, 2314
BmrI	ACTGGG	2 cut(s)	128, 475
BmuI	ACTGGG	2 cut(s)	128, 475
BpiI	GAAGAC	3 cut(s)	393, 1263, 2367
BpmI	CTGGAG	5 cut(s)	56, 188, 758, 782, 2217
Bpu10I	CCTNAGC	1 cut(s)	1248
Bpu1102I	GCTNAGC	1 cut(s)	2078
Bpu14I	TTCGAA	2 cut(s)	153, 1287
BpuMI	CCSGG	4 cut(s)	241, 322, 928, 2314
Bsa29I	ATCGAT	1 cut(s)	315
BsaBI	GATNNNNATC	1 cut(s)	1266
BsaJI	CCNNGG	3 cut(s)	1020, 2088, 2313
BsaWI	WCCGGW	1 cut(s)	49
Bse1I	ACTGG	7 cut(s)	39, 134, 470, 765, 1354, 1367, 2166
Bse3DI	GCAATG	1 cut(s)	400
Bse8I	GATNNNNATC	1 cut(s)	1266
BseBI	CCWGG	1 cut(s)	167
BseCI	ATCGAT	1 cut(s)	315
BseDI	CCNNGG	3 cut(s)	1020, 2088, 2313
BseGI	GGATG	9 cut(s)	936, 940, 1030, 1243, 1315, 1426, 1774, 1898, 1937
BseJI	GATNNNNATC	1 cut(s)	1266
BseMI	GCAATG	1 cut(s)	400
BseMII	CTCAG	7 cut(s)	674, 810, 851, 998, 1239, 1716, 1946
BseNI	ACTGG	7 cut(s)	39, 134, 470, 765, 1354, 1367, 2166
BseRI	GAGGAG	7 cut(s)	36, 449, 836, 911, 1104, 1154, 1887
BseSI	GKGCMC	1 cut(s)	2179
BseXI	GCAGC	6 cut(s)	28, 574, 602, 701, 1586, 2296
Bsh1236I	CGCG	1 cut(s)	57
BshFI	GGCC	4 cut(s)	132, 575, 1366, 1835
BshVI	ATCGAT	1 cut(s)	315
BsiHKAI	GWGCWC	4 cut(s)	970, 1961, 2009, 2179
BsiHKCI	CYCGRG	1 cut(s)	1409
BsiSI	CCGG	6 cut(s)	50, 240, 321, 476, 927, 2313
BslFI	GGGAC	2 cut(s)	1524, 2254
BsmAI	GTCTC	7 cut(s)	3, 500, 658, 1311, 1685, 1696, 1869
BsmBI	CGTCTC	2 cut(s)	3, 500
BsmFI	GGGAC	2 cut(s)	1524, 2254
BsmI	GAATGC	1 cut(s)	901
BsnI	GGCC	4 cut(s)	132, 575, 1366, 1835
BsoBI	CYCGRG	1 cut(s)	1409
Bsp119I	TTCGAA	2 cut(s)	153, 1287
Bsp1286I	GDGCHC	4 cut(s)	970, 1961, 2009, 2179
Bsp143I	GATC	8 cut(s)	203, 457, 1027, 1042, 1216, 1267, 2189, 2258
Bsp1720I	GCTNAGC	1 cut(s)	2078
BspANI	GGCC	4 cut(s)	132, 575, 1366, 1835
BspCNI	CTCAG	7 cut(s)	673, 811, 850, 997, 1240, 1717, 1947
BspDI	ATCGAT	1 cut(s)	315
BspFNI	CGCG	1 cut(s)	57
BspHI	TCATGA	1 cut(s)	1270
BspLI	GGNNCC	3 cut(s)	286, 825, 948
BspMAI	CTGCAG	1 cut(s)	592
BspMI	ACCTGC	1 cut(s)	1093
BspPI	GGATC	4 cut(s)	211, 1037, 1211, 2266
BspQI	GCTCTTC	3 cut(s)	96, 557, 1548
BspT104I	TTCGAA	2 cut(s)	153, 1287
BsrBI	CCGCTC	1 cut(s)	2201
BsrDI	GCAATG	1 cut(s)	400
BsrI	ACTGG	7 cut(s)	39, 134, 470, 765, 1354, 1367, 2166
BssECI	CCNNGG	3 cut(s)	1020, 2088, 2313
BssMI	GATC	8 cut(s)	203, 457, 1027, 1042, 1216, 1267, 2189, 2258
BssNAI	GTATAC	3 cut(s)	1650, 1669, 2398
BssT1I	CCWWGG	2 cut(s)	1020, 2088
Bst1107I	GTATAC	3 cut(s)	1650, 1669, 2398
Bst2UI	CCWGG	1 cut(s)	167
Bst6I	CTCTTC	9 cut(s)	96, 426, 498, 543, 557, 1056, 1488, 1518, 1548
BstAPI	GCANNNNNTGC	1 cut(s)	1636
BstBI	TTCGAA	2 cut(s)	153, 1287
BstC8I	GCNNGC	5 cut(s)	651, 1743, 1866, 2211, 2382
BstF5I	GGATG	9 cut(s)	936, 940, 1030, 1243, 1315, 1426, 1774, 1898, 1937
BstFNI	CGCG	1 cut(s)	57
BstH2I	RGCGCY	1 cut(s)	76
BstHHI	GCGC	3 cut(s)	75, 1819, 2209
BstKTI	GATC	8 cut(s)	206, 460, 1030, 1045, 1219, 1270, 2192, 2261
BstMAI	GTCTC	7 cut(s)	3, 500, 658, 1311, 1685, 1696, 1869
BstMBI	GATC	8 cut(s)	203, 457, 1027, 1042, 1216, 1267, 2189, 2258
BstMWI	GCNNNNNNNGC	2 cut(s)	640, 1636
BstNI	CCWGG	1 cut(s)	167
BstNSI	RCATGY	2 cut(s)	653, 2396
BstSCI	CCNGG	5 cut(s)	165, 239, 320, 926, 2312
BstSFI	CTRYAG	2 cut(s)	588, 2067
BstSLI	GKGCMC	1 cut(s)	2179
BstUI	CGCG	1 cut(s)	57
BstV1I	GCAGC	6 cut(s)	28, 574, 602, 701, 1586, 2296
BstV2I	GAAGAC	3 cut(s)	393, 1263, 2367
BstX2I	RGATCY	1 cut(s)	2258
BstYI	RGATCY	1 cut(s)	2258
BstZ17I	GTATAC	3 cut(s)	1650, 1669, 2398
Bsu15I	ATCGAT	1 cut(s)	315
BsuRI	GGCC	4 cut(s)	132, 575, 1366, 1835
BsuTUI	ATCGAT	1 cut(s)	315
BtgZI	GCGATG	1 cut(s)	2320
BtrI	CACGTC	1 cut(s)	1195
BtsCI	GGATG	9 cut(s)	936, 940, 1030, 1243, 1315, 1426, 1774, 1898, 1937
BtsI	GCAGTG	1 cut(s)	1320
BtsIMutI	CAGTG	5 cut(s)	32, 141, 1320, 1721, 2159
BveI	ACCTGC	1 cut(s)	1093
Cac8I	GCNNGC	5 cut(s)	651, 1743, 1866, 2211, 2382
CaiI	CAGNNNCTG	3 cut(s)	991, 1453, 1720
CciI	TCATGA	1 cut(s)	1270
CfoI	GCGC	3 cut(s)	75, 1819, 2209
Cfr13I	GGNCC	4 cut(s)	52, 131, 573, 988
ClaI	ATCGAT	1 cut(s)	315
CpoI	CGGWCCG	1 cut(s)	52
Csp6I	GTAC	3 cut(s)	786, 803, 1982
CspI	CGGWCCG	1 cut(s)	52
CviAII	CATG	6 cut(s)	556, 650, 1090, 1271, 1949, 2393
CviQI	GTAC	3 cut(s)	786, 803, 1982
DpnI	GATC	8 cut(s)	205, 459, 1029, 1044, 1218, 1269, 2191, 2260
DpnII	GATC	8 cut(s)	203, 457, 1027, 1042, 1216, 1267, 2189, 2258
DraI	TTTAAA	1 cut(s)	697
Eam1104I	CTCTTC	9 cut(s)	96, 426, 498, 543, 557, 1056, 1488, 1518, 1548
EarI	CTCTTC	9 cut(s)	96, 426, 498, 543, 557, 1056, 1488, 1518, 1548
EciI	GGCGGA	2 cut(s)	1399, 2228
Ecl136II	GAGCTC	1 cut(s)	2007
Eco130I	CCWWGG	2 cut(s)	1020, 2088
Eco24I	GRGCYC	1 cut(s)	2009
Eco32I	GATATC	1 cut(s)	726
Eco47I	GGWCC	2 cut(s)	52, 988
Eco47III	AGCGCT	1 cut(s)	74
Eco53kI	GAGCTC	1 cut(s)	2007
Eco57I	CTGAAG	8 cut(s)	89, 659, 714, 1203, 1475, 2010, 2157, 2247
Eco88I	CYCGRG	1 cut(s)	1409
EcoICRI	GAGCTC	1 cut(s)	2007
EcoO109I	RGGNCCY	1 cut(s)	988
EcoRI	GAATTC	1 cut(s)	149
EcoRII	CCWGG	1 cut(s)	165
EcoRV	GATATC	1 cut(s)	726
EcoT14I	CCWWGG	2 cut(s)	1020, 2088
EcoT38I	GRGCYC	1 cut(s)	2009
ErhI	CCWWGG	2 cut(s)	1020, 2088
Esp3I	CGTCTC	2 cut(s)	3, 500
FaeI	CATG	6 cut(s)	559, 653, 1093, 1274, 1952, 2396
FalI	AAGNNNNNCTT	4 cut(s)	1886, 1918, 2272, 2304
FaqI	GGGAC	2 cut(s)	1524, 2254
FatI	CATG	6 cut(s)	555, 649, 1089, 1270, 1948, 2392
FauI	CCCGC	2 cut(s)	2194, 2373
FbaI	TGATCA	1 cut(s)	1267
FblI	GTMKAC	3 cut(s)	1649, 1668, 2397
Fnu4HI	GCNGC	9 cut(s)	42, 220, 379, 588, 591, 690, 813, 1575, 2285
FokI	GGATG	9 cut(s)	943, 947, 1037, 1250, 1322, 1433, 1781, 1885, 1924
FriOI	GRGCYC	1 cut(s)	2009
Fsp4HI	GCNGC	9 cut(s)	42, 220, 379, 588, 591, 690, 813, 1575, 2285
FspBI	CTAG	1 cut(s)	2410
FspI	TGCGCA	1 cut(s)	2208
GlaI	GCGC	3 cut(s)	74, 1818, 2208
GluI	GCNGC	9 cut(s)	42, 220, 379, 588, 591, 690, 813, 1575, 2285
GsuI	CTGGAG	5 cut(s)	56, 188, 758, 782, 2217
HaeII	RGCGCY	1 cut(s)	76
HaeIII	GGCC	4 cut(s)	132, 575, 1366, 1835
HapII	CCGG	6 cut(s)	50, 240, 321, 476, 927, 2313
HhaI	GCGC	3 cut(s)	75, 1819, 2209
Hin1II	CATG	6 cut(s)	559, 653, 1093, 1274, 1952, 2396
Hin6I	GCGC	3 cut(s)	73, 1817, 2207
HinP1I	GCGC	3 cut(s)	73, 1817, 2207
HincII	GTYRAC	3 cut(s)	115, 940, 2329
HindII	GTYRAC	3 cut(s)	115, 940, 2329
HindIII	AAGCTT	2 cut(s)	1227, 2075
HpaII	CCGG	6 cut(s)	50, 240, 321, 476, 927, 2313
Hpy166II	GTNNAC	7 cut(s)	115, 940, 1650, 1669, 2177, 2329, 2398
Hpy188III	TCNNGA	7 cut(s)	737, 986, 1118, 1181, 1271, 1428, 1779
Hpy8I	GTNNAC	7 cut(s)	115, 940, 1650, 1669, 2177, 2329, 2398
Hpy99I	CGWCG	2 cut(s)	190, 293
HpyCH4IV	ACGT	1 cut(s)	1194
HpyF10VI	GCNNNNNNNGC	2 cut(s)	640, 1636
HpySE526I	ACGT	1 cut(s)	1194
Hsp92II	CATG	6 cut(s)	559, 653, 1093, 1274, 1952, 2396
HspAI	GCGC	3 cut(s)	73, 1817, 2207
Ksp22I	TGATCA	1 cut(s)	1267
Kzo9I	GATC	8 cut(s)	203, 457, 1027, 1042, 1216, 1267, 2189, 2258
LguI	GCTCTTC	3 cut(s)	96, 557, 1548
Lsp1109I	GCAGC	6 cut(s)	28, 574, 602, 701, 1586, 2296
MaeI	CTAG	1 cut(s)	2410
MaeII	ACGT	1 cut(s)	1194
MaeIII	GTNAC	5 cut(s)	30, 1755, 1988, 2115, 2265
MalI	GATC	8 cut(s)	205, 459, 1029, 1044, 1218, 1269, 2191, 2260
MbiI	CCGCTC	1 cut(s)	2201
MboI	GATC	8 cut(s)	203, 457, 1027, 1042, 1216, 1267, 2189, 2258
MfeI	CAATTG	1 cut(s)	828
MflI	RGATCY	1 cut(s)	2258
MhlI	GDGCHC	4 cut(s)	970, 1961, 2009, 2179
MluCI	AATT	4 cut(s)	87, 149, 361, 828
MlyI	GAGTC	2 cut(s)	1010, 1100
MmeI	TCCRAC	2 cut(s)	312, 1995
MseI	TTAA	5 cut(s)	77, 696, 1097, 1332, 2279
MslI	CAYNNNNRTG	1 cut(s)	775
MspA1I	CMGCKG	5 cut(s)	593, 1057, 2195, 2225, 2255
MspI	CCGG	6 cut(s)	50, 240, 321, 476, 927, 2313
MspR9I	CCNGG	5 cut(s)	167, 241, 322, 928, 2314
MunI	CAATTG	1 cut(s)	828
Mva1269I	GAATGC	1 cut(s)	901
MvaI	CCWGG	1 cut(s)	167
MvnI	CGCG	1 cut(s)	57
MwoI	GCNNNNNNNGC	2 cut(s)	640, 1636
NciI	CCSGG	4 cut(s)	241, 322, 928, 2314
NdeII	GATC	8 cut(s)	203, 457, 1027, 1042, 1216, 1267, 2189, 2258
NlaIII	CATG	6 cut(s)	559, 653, 1093, 1274, 1952, 2396
NlaIV	GGNNCC	3 cut(s)	286, 825, 948
NmuCI	GTSAC	4 cut(s)	30, 1755, 1988, 2115
NsbI	TGCGCA	1 cut(s)	2208
NspI	RCATGY	2 cut(s)	653, 2396
NspV	TTCGAA	2 cut(s)	153, 1287
PaeI	GCATGC	1 cut(s)	653
PaeR7I	CTCGAG	1 cut(s)	1409
PagI	TCATGA	1 cut(s)	1270
PciI	ACATGT	1 cut(s)	2392
PciSI	GCTCTTC	3 cut(s)	96, 557, 1548
PcsI	WCGNNNNNNNCGW	2 cut(s)	182, 2381
PctI	GAATGC	1 cut(s)	901
PfoI	TCCNGGA	1 cut(s)	926
PkrI	GCNGC	9 cut(s)	43, 221, 380, 589, 592, 691, 814, 1576, 2286
PleI	GAGTC	2 cut(s)	1010, 1100
PpsI	GAGTC	2 cut(s)	1010, 1100
PpuMI	RGGWCCY	1 cut(s)	988
PscI	ACATGT	1 cut(s)	2392
PsiI	TTATAA	1 cut(s)	798
Psp124BI	GAGCTC	1 cut(s)	2009
Psp5II	RGGWCCY	1 cut(s)	988
Psp6I	CCWGG	1 cut(s)	165
PspGI	CCWGG	1 cut(s)	165
PspN4I	GGNNCC	3 cut(s)	286, 825, 948
PspPI	GGNCC	4 cut(s)	52, 131, 573, 988
PspPPI	RGGWCCY	1 cut(s)	988
PspXI	VCTCGAGB	1 cut(s)	1409
PstI	CTGCAG	1 cut(s)	592
PstNI	CAGNNNCTG	3 cut(s)	991, 1453, 1720
PsuI	RGATCY	1 cut(s)	2258
PvuII	CAGCTG	4 cut(s)	593, 2195, 2225, 2255
RsaI	GTAC	3 cut(s)	787, 804, 1983
RsaNI	GTAC	3 cut(s)	786, 803, 1982
RseI	CAYNNNNRTG	1 cut(s)	775
Rsr2I	CGGWCCG	1 cut(s)	52
RsrII	CGGWCCG	1 cut(s)	52
SacI	GAGCTC	1 cut(s)	2009
SapI	GCTCTTC	3 cut(s)	96, 557, 1548
SaqAI	TTAA	5 cut(s)	77, 696, 1097, 1332, 2279
SatI	GCNGC	9 cut(s)	42, 220, 379, 588, 591, 690, 813, 1575, 2285
Sau3AI	GATC	8 cut(s)	203, 457, 1027, 1042, 1216, 1267, 2189, 2258
Sau96I	GGNCC	4 cut(s)	52, 131, 573, 988
SchI	GAGTC	2 cut(s)	1010, 1100
ScrFI	CCNGG	5 cut(s)	167, 241, 322, 928, 2314
SduI	GDGCHC	4 cut(s)	970, 1961, 2009, 2179
SfcI	CTRYAG	2 cut(s)	588, 2067
Sfr274I	CTCGAG	1 cut(s)	1409
SfuI	TTCGAA	2 cut(s)	153, 1287
SinI	GGWCC	2 cut(s)	52, 988
SlaI	CTCGAG	1 cut(s)	1409
SmiMI	CAYNNNNRTG	1 cut(s)	775
SmlI	CTYRAG	1 cut(s)	1409
SmoI	CTYRAG	1 cut(s)	1409
SphI	GCATGC	1 cut(s)	653
Sse9I	AATT	4 cut(s)	87, 149, 361, 828
SspI	AATATT	2 cut(s)	1165, 1813
SspMI	CTAG	1 cut(s)	2410
SstI	GAGCTC	1 cut(s)	2009
StyD4I	CCNGG	5 cut(s)	165, 239, 320, 926, 2312
StyI	CCWWGG	2 cut(s)	1020, 2088
TaiI	ACGT	1 cut(s)	1197
TasI	AATT	4 cut(s)	87, 149, 361, 828
TatI	WGTACW	1 cut(s)	785
TauI	GCSGC	3 cut(s)	222, 381, 815
Tru1I	TTAA	5 cut(s)	77, 696, 1097, 1332, 2279
Tru9I	TTAA	5 cut(s)	77, 696, 1097, 1332, 2279
TscAI	CASTG	5 cut(s)	39, 141, 1327, 1721, 2166
TseFI	GTSAC	4 cut(s)	30, 1755, 1988, 2115
TseI	GCWGC	6 cut(s)	41, 587, 590, 689, 1574, 2284
Tsp45I	GTSAC	4 cut(s)	30, 1755, 1988, 2115
TspGWI	ACGGA	4 cut(s)	524, 2332, 2379, 2403
TspRI	CASTG	5 cut(s)	39, 141, 1327, 1721, 2166
VneI	GTGCAC	1 cut(s)	2175
VpaK11BI	GGWCC	2 cut(s)	52, 988
XapI	RAATTY	2 cut(s)	149, 361
XceI	RCATGY	2 cut(s)	653, 2396
XhoI	CTCGAG	1 cut(s)	1409
XmiI	GTMKAC	3 cut(s)	1649, 1668, 2397
XspI	CTAG	1 cut(s)	2410

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.