Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0011941

Physical Location & Seq

Genomic Coordinates Forward (+)

4114123 .. 4117779

Gene Structure 3657 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1052400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1626 bp

ATGCATAACCCTTCAGTCGCAACCCAGGAACCCAGTCCGGTCTACGACCTCGTTTCGCAATTGGGTCGGTTCGCCTTCAGCTCAGGGCCTCATAGCAGCTCCGGTTCTGATGGTCACTCCTCGTTTTTGTCCAATGTCTTGTATTTCGACGCCTTTGGGGGTGCTTCAGTTGCCGGGAAGAAGCCCAAGGTCGGCGTTTCAGCTTCACTGAGCTTGAGCAGTCGCGGCGGTTTGGGAATTTGGAGGAGTGTGACGGAGTTTAATCGGGCTATACGGTTCCACTGTGACAAATTCCCGATTGGGTTTGCTTCTGTTCGCGTCGGGTCTGGGGACGGAAATGGAAATGGGTCGGATAACGGCGTTGGAGACAATAACAATGTGTTGGTAGAAGATGGAGGCGGAGTTAACGATAATGGGATTGCGTTGAATGGTGTGAAGGTGGAGAGACCCAAGAAAGTTTTGATTTTGATGAGTGACACCGGCGGCGGCCACCGTGCTTCTGCGGAGGCGATTAAGGCGGCGTTTAATGAGGAATTTGGTGATGAATATCAGGTGTTTGTTACTGACTTATGGTCGGATCATACGCCTTGGCCATTTAATCAACTTCCCAGGAGCTATAATTTCTTGGTGAAACACGGGCCGTTATGGAAAATGACATATTATGGGACTGCTCCGCGGCTGGTGCATCAATCTAATTTTGCAGCAACTTCTACGTTTATAGCTCGGGAGGTTGCAAAAGGACTGATGAAATACCAGCCGGATATTATCATCAGTGTGCATCCGCTGATGCAACATGTACCACTTCGTGTCTTGAGGGGAAGGGGTCTATTGAAGAAGATAGTCTTTACCACAGTTGTCACGGATTTAAGTACTTGTCATCCTACATGGTTTCATAAGCTTGTAACGAGATGCTATTGCCCAACAAATGAGGTCGCAAAGAGGGCATTGAAAGCTGGACTCCAGCCTTCCCAAATCAAGGTTTATGGCCTCCCTGTGCGGCCTAGCTTTGTCAAGCCTGTGCGACCAAAGGCTGAACTTCGGAGAGAACTAGGAATGGAGGAGGATCTTCCCGCTGTGTTGCTCATGGGAGGAGGAGAAGGAATGGGTCCTATCGAAGCTACAGCTCAGGAACTTGGGGATGCATTATATGACGAGAATCTTGGGGAGCCTGTAGGTCAGGTCCTTGTCATATGTGGTCGCAACAAAAAGCTTGCAAACAAGTTGCTTTCTATCAACTGGAAGATCCCGGTCCAGGTGAAGGGGTTTGTTACTAAAATGGAAGAATGCATGGGAGCTTGCGATTGCATTATTACGAAGGCCGGTCCGGGGACTATTGCAGAAGCCATGATAAGAGGCCTCCCGATTATTCTGAACGATTACATTGCCGGACAGGAAGTTGGGAATGTACCATATGTCGTTGAAAACGGGTGCGGAAAATATTCTAAATCTCCGAAAGAGATAGCAAGGATCGTCGCTGATTGGTTTGGTCCGAAAGCAGATGAGCTAAAGGCCATGTCGGAAAATGCACTGAAGCTGGCAAAGCCTGATTCCGTGTTCAAGATTGTTCATGATCTTCACGAGCTCGTTAGACAACGAAGCCTTGTACCGCAATTATGTACAACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006643 GO:0006664 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0008378 GO:0008610 GO:0009058 GO:0009247 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009657 GO:0009668 GO:0009706 GO:0009790 GO:0009791 GO:0009793 GO:0009941 GO:0009987 GO:0010027 GO:0010154 GO:0016020 GO:0016043 GO:0016740 GO:0016757 GO:0016758 GO:0019866 GO:0022414 GO:0031090 GO:0031967 GO:0031969 GO:0031975 GO:0032501 GO:0032502 GO:0035250 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046467 GO:0046509 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061024 GO:0061458 GO:0071704 GO:0071840 GO:1901135 GO:1901137 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00561 ko01100 map00561 map01100

Pfam Domains

Protein Families

Glyco_tran_28_C MGDG_synth

Protein Analysis

542

Amino Acids

58.73

Weight (kDa)

8.83

Isoelectric Point (pI)

37.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MGDG_synth	PF06925	164 - 332	4.7e-63	Monogalactosyldiacylglycerol (MGDG) synthase
Glyco_tran_28_C	PF04101	360 - 480	1.1e-09	Glycosyltransferase family 28 C-terminal domain
Glycos_transf_1	PF00534	394 - 510	3.7e-06	Glycosyl transferases group 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011941)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31780 AT4G31780 AT4G31780
fragaria_vesca	FvH4_2g41310
malus_domestica	MD08G1052400.v1.1 MD15G1043100.v1.1
prunus_persica	Prupe.1G396500_v2.0.a1
pyrus_communis	pycom08g04370 pycom15g04010
rosa_chinensis	RchiOBHm_Chr6g0299211
rosa_laevigata	RLG00000011453
rosa_roxburghii	Rroxscaffold_7G00168910
rosa_rugosa	Rorug06G0286300 Rorug06G0286300
rosa_samantha	Rh6AG397400 Rh6BG406000 Rh6CG412100 Rh6DG398800
rosa_wichuraiana	Rw6G034790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	42
AccII	CGCG	3 cut(s)	225, 318, 676
AclWI	GGATC	4 cut(s)	585, 1071, 1237, 1475
AcoI	YGGCCR	2 cut(s)	487, 590
AcsI	RAATTY	3 cut(s)	237, 290, 533
AcuI	CTGAAG	3 cut(s)	61, 150, 1550
AcyI	GRCGYC	1 cut(s)	150
AdeI	CACNNNGTG	1 cut(s)	806
AfaI	GTAC	5 cut(s)	798, 871, 1407, 1605, 1618
AfiI	CCNNNNNNNGG	5 cut(s)	191, 976, 1252, 1258, 1326
AflIII	ACRYGT	1 cut(s)	793
AgsI	TTSAA	5 cut(s)	427, 832, 949, 1421, 1558
AhdI	GACNNNNNGTC	1 cut(s)	571
AjnI	CCWGG	3 cut(s)	24, 608, 1251
Alw21I	GWGCWC	1 cut(s)	1584
Alw26I	GTCTC	2 cut(s)	360, 439
AlwI	GGATC	4 cut(s)	585, 1071, 1237, 1475
Ama87I	CYCGRG	1 cut(s)	723
AoxI	GGCC	9 cut(s)	86, 487, 590, 638, 985, 998, 1317, 1354, 1509
ApeKI	GCWGC	2 cut(s)	96, 701
ApoI	RAATTY	3 cut(s)	237, 290, 533
Asp700I	GAANNNNTTC	1 cut(s)	1438
AspS9I	GGNCC	7 cut(s)	86, 638, 1106, 1180, 1249, 1322, 1487
AsuC2I	CCSGG	3 cut(s)	175, 1247, 1326
AsuHPI	GGTGA	3 cut(s)	551, 640, 1267
AvaI	CYCGRG	1 cut(s)	723
AvaII	GGWCC	5 cut(s)	1106, 1180, 1249, 1322, 1487
BalI	TGGCCA	1 cut(s)	592
BanII	GRGCYC	1 cut(s)	1584
BauI	CACGAG	1 cut(s)	1577
Bbv12I	GWGCWC	1 cut(s)	1584
BbvI	GCAGC	2 cut(s)	108, 713
BccI	CCATC	2 cut(s)	104, 386
BceAI	ACGGC	2 cut(s)	373, 625
BcgI	CGANNNNNNTGC	4 cut(s)	47, 81, 1364, 1398
BciT130I	CCWGG	3 cut(s)	26, 610, 1253
BcnI	CCSGG	3 cut(s)	175, 1247, 1326
BcoDI	GTCTC	2 cut(s)	360, 439
BfaI	CTAG	2 cut(s)	1002, 1049
BfmI	CTRYAG	2 cut(s)	1119, 1170
BisI	GCNGC	8 cut(s)	97, 226, 484, 487, 519, 677, 702, 998
BlsI	GCNGC	8 cut(s)	98, 227, 485, 488, 520, 678, 703, 999
BmcAI	AGTACT	1 cut(s)	871
Bme1390I	CCNGG	6 cut(s)	26, 175, 610, 1247, 1253, 1326
Bme18I	GGWCC	5 cut(s)	1106, 1180, 1249, 1322, 1487
BmeRI	GACNNNNNGTC	1 cut(s)	571
BmeT110I	CYCGRG	1 cut(s)	723
BmgT120I	GGNCC	7 cut(s)	86, 638, 1106, 1180, 1249, 1322, 1487
BmiI	GGNNCC	4 cut(s)	30, 278, 1107, 1167
BmrFI	CCNGG	6 cut(s)	26, 175, 610, 1247, 1253, 1326
BmrI	ACTGGG	1 cut(s)	27
BmsI	GCATC	5 cut(s)	694, 777, 787, 899, 1129
BmuI	ACTGGG	1 cut(s)	27
BpmI	CTGGAG	1 cut(s)	944
Bpu10I	CCTNAGC	2 cut(s)	82, 1125
BpuEI	CTTGAG	2 cut(s)	235, 832
BpuMI	CCSGG	3 cut(s)	175, 1247, 1326
BsaBI	GATNNNNATC	1 cut(s)	546
BsaHI	GRCGYC	1 cut(s)	150
BsaI	GGTCTC	1 cut(s)	439
BsaJI	CCNNGG	6 cut(s)	24, 186, 587, 608, 674, 1325
BsaWI	WCCGGW	2 cut(s)	37, 101
BsaXI	ACNNNNNCTCC	2 cut(s)	387, 417
Bsc4I	CCNNNNNNNGG	5 cut(s)	191, 976, 1252, 1258, 1326
Bse118I	RCCGGY	2 cut(s)	479, 1319
Bse1I	ACTGG	2 cut(s)	33, 1241
Bse3DI	GCAATG	1 cut(s)	1380
Bse8I	GATNNNNATC	1 cut(s)	546
BseBI	CCWGG	3 cut(s)	26, 610, 1253
BseDI	CCNNGG	6 cut(s)	24, 186, 587, 608, 674, 1325
BseGI	GGATG	3 cut(s)	778, 877, 1144
BseJI	GATNNNNATC	1 cut(s)	546
BseLI	CCNNNNNNNGG	5 cut(s)	191, 976, 1252, 1258, 1326
BseMI	GCAATG	1 cut(s)	1380
BseMII	CTCAG	3 cut(s)	96, 200, 1139
BseNI	ACTGG	2 cut(s)	33, 1241
BseRI	GAGGAG	5 cut(s)	109, 259, 1073, 1104, 1107
BseXI	GCAGC	2 cut(s)	108, 713
Bsh1236I	CGCG	3 cut(s)	225, 318, 676
BshFI	GGCC	9 cut(s)	88, 489, 592, 640, 987, 1000, 1319, 1356, 1511
BsiHKAI	GWGCWC	1 cut(s)	1584
BsiHKCI	CYCGRG	1 cut(s)	723
BsiSI	CCGG	9 cut(s)	38, 102, 174, 480, 758, 1247, 1320, 1325, 1386
BslFI	GGGAC	3 cut(s)	344, 679, 1342
BslI	CCNNNNNNNGG	5 cut(s)	191, 976, 1252, 1258, 1326
BsmAI	GTCTC	2 cut(s)	360, 439
BsmFI	GGGAC	3 cut(s)	344, 679, 1342
BsmI	GAATGC	1 cut(s)	1289
BsnI	GGCC	9 cut(s)	88, 489, 592, 640, 987, 1000, 1319, 1356, 1511
Bso31I	GGTCTC	1 cut(s)	439
BsoBI	CYCGRG	1 cut(s)	723
Bsp1286I	GDGCHC	1 cut(s)	1584
Bsp1407I	TGTACA	1 cut(s)	1616
Bsp143I	GATC	5 cut(s)	577, 1063, 1242, 1467, 1570
BspANI	GGCC	9 cut(s)	88, 489, 592, 640, 987, 1000, 1319, 1356, 1511
BspCNI	CTCAG	3 cut(s)	95, 201, 1138
BspFNI	CGCG	3 cut(s)	225, 318, 676
BspHI	TCATGA	1 cut(s)	1567
BspLI	GGNNCC	4 cut(s)	30, 278, 1107, 1167
BspPI	GGATC	4 cut(s)	585, 1071, 1237, 1475
BspTNI	GGTCTC	1 cut(s)	439
BsrDI	GCAATG	1 cut(s)	1380
BsrFI	RCCGGY	2 cut(s)	479, 1319
BsrGI	TGTACA	1 cut(s)	1616
BsrI	ACTGG	2 cut(s)	33, 1241
BssAI	RCCGGY	2 cut(s)	479, 1319
BssECI	CCNNGG	6 cut(s)	24, 186, 587, 608, 674, 1325
BssMI	GATC	5 cut(s)	577, 1063, 1242, 1467, 1570
BssNI	GRCGYC	1 cut(s)	150
BssSI	CACGAG	1 cut(s)	1577
BssT1I	CCWWGG	2 cut(s)	186, 587
Bst2BI	CACGAG	1 cut(s)	1577
Bst2UI	CCWGG	3 cut(s)	26, 610, 1253
Bst4CI	ACNGT	4 cut(s)	276, 284, 494, 853
BstACI	GRCGYC	1 cut(s)	150
BstAUI	TGTACA	1 cut(s)	1616
BstC8I	GCNNGC	3 cut(s)	1212, 1297, 1536
BstDEI	CTNAG	3 cut(s)	82, 209, 1125
BstDSI	CCRYGG	1 cut(s)	674
BstF5I	GGATG	3 cut(s)	778, 877, 1144
BstFNI	CGCG	3 cut(s)	225, 318, 676
BstKTI	GATC	5 cut(s)	580, 1066, 1245, 1470, 1573
BstMAI	GTCTC	2 cut(s)	360, 439
BstMBI	GATC	5 cut(s)	577, 1063, 1242, 1467, 1570
BstMWI	GCNNNNNNNGC	7 cut(s)	170, 225, 515, 682, 941, 950, 1540
BstNI	CCWGG	3 cut(s)	26, 610, 1253
BstNSI	RCATGY	1 cut(s)	797
BstSCI	CCNGG	6 cut(s)	24, 173, 608, 1245, 1251, 1324
BstSFI	CTRYAG	2 cut(s)	1119, 1170
BstUI	CGCG	3 cut(s)	225, 318, 676
BstV1I	GCAGC	2 cut(s)	108, 713
BstX2I	RGATCY	2 cut(s)	1063, 1242
BstYI	RGATCY	2 cut(s)	1063, 1242
BsuRI	GGCC	9 cut(s)	88, 489, 592, 640, 987, 1000, 1319, 1356, 1511
BtgI	CCRYGG	1 cut(s)	674
BtsCI	GGATG	3 cut(s)	778, 877, 1144
BtsIMutI	CAGTG	4 cut(s)	206, 280, 778, 1526
Cac8I	GCNNGC	3 cut(s)	1212, 1297, 1536
CciI	TCATGA	1 cut(s)	1567
Cfr10I	RCCGGY	2 cut(s)	479, 1319
Cfr13I	GGNCC	7 cut(s)	86, 638, 1106, 1180, 1249, 1322, 1487
Cfr42I	CCGCGG	1 cut(s)	677
CpoI	CGGWCCG	1 cut(s)	1322
CseI	GACGC	2 cut(s)	158, 307
Csp6I	GTAC	5 cut(s)	797, 870, 1406, 1604, 1617
CspI	CGGWCCG	1 cut(s)	1322
CviAII	CATG	7 cut(s)	794, 885, 1084, 1288, 1345, 1513, 1568
CviQI	GTAC	5 cut(s)	797, 870, 1406, 1604, 1617
DdeI	CTNAG	3 cut(s)	82, 209, 1125
DpnI	GATC	5 cut(s)	579, 1065, 1244, 1469, 1572
DpnII	GATC	5 cut(s)	577, 1063, 1242, 1467, 1570
DraIII	CACNNNGTG	1 cut(s)	806
DriI	GACNNNNNGTC	1 cut(s)	571
EaeI	YGGCCR	2 cut(s)	487, 590
Eam1105I	GACNNNNNGTC	1 cut(s)	571
EciI	GGCGGA	1 cut(s)	414
Ecl136II	GAGCTC	1 cut(s)	1582
Eco130I	CCWWGG	2 cut(s)	186, 587
Eco147I	AGGCCT	1 cut(s)	1356
Eco24I	GRGCYC	1 cut(s)	1584
Eco31I	GGTCTC	1 cut(s)	439
Eco47I	GGWCC	5 cut(s)	1106, 1180, 1249, 1322, 1487
Eco53kI	GAGCTC	1 cut(s)	1582
Eco57I	CTGAAG	3 cut(s)	61, 150, 1550
Eco88I	CYCGRG	1 cut(s)	723
EcoICRI	GAGCTC	1 cut(s)	1582
EcoO109I	RGGNCCY	3 cut(s)	86, 1106, 1180
EcoRII	CCWGG	3 cut(s)	24, 608, 1251
EcoT14I	CCWWGG	2 cut(s)	186, 587
EcoT22I	ATGCAT	3 cut(s)	6, 1144, 1289
EcoT38I	GRGCYC	1 cut(s)	1584
ErhI	CCWWGG	2 cut(s)	186, 587
FaeI	CATG	7 cut(s)	797, 888, 1087, 1291, 1348, 1516, 1571
FalI	AAGNNNNNCTT	2 cut(s)	827, 859
FaqI	GGGAC	3 cut(s)	344, 679, 1342
FatI	CATG	7 cut(s)	793, 884, 1083, 1287, 1344, 1512, 1567
FauI	CCCGC	1 cut(s)	1078
FauNDI	CATATG	2 cut(s)	1190, 1411
FblI	GTMKAC	1 cut(s)	42
Fnu4HI	GCNGC	8 cut(s)	97, 226, 484, 487, 519, 677, 702, 998
FokI	GGATG	3 cut(s)	765, 864, 1151
FriOI	GRGCYC	1 cut(s)	1584
Fsp4HI	GCNGC	8 cut(s)	97, 226, 484, 487, 519, 677, 702, 998
FspBI	CTAG	2 cut(s)	1002, 1049
GluI	GCNGC	8 cut(s)	97, 226, 484, 487, 519, 677, 702, 998
GsuI	CTGGAG	1 cut(s)	944
HaeIII	GGCC	9 cut(s)	88, 489, 592, 640, 987, 1000, 1319, 1356, 1511
HapII	CCGG	9 cut(s)	38, 102, 174, 480, 758, 1247, 1320, 1325, 1386
HgaI	GACGC	2 cut(s)	158, 307
Hin1I	GRCGYC	1 cut(s)	150
Hin1II	CATG	7 cut(s)	797, 888, 1087, 1291, 1348, 1516, 1571
HincII	GTYRAC	1 cut(s)	406
HindII	GTYRAC	1 cut(s)	406
HindIII	AAGCTT	2 cut(s)	896, 1208
HinfI	GANTC	3 cut(s)	957, 1156, 1547
HpaI	GTTAAC	1 cut(s)	406
HpaII	CCGG	9 cut(s)	38, 102, 174, 480, 758, 1247, 1320, 1325, 1386
HphI	GGTGA	3 cut(s)	551, 640, 1267
Hpy166II	GTNNAC	2 cut(s)	43, 406
Hpy188I	TCNGA	8 cut(s)	109, 352, 577, 1041, 1371, 1452, 1491, 1519
Hpy188III	TCNNGA	8 cut(s)	295, 725, 811, 1127, 1360, 1558, 1568, 1577
Hpy8I	GTNNAC	2 cut(s)	43, 406
Hpy99I	CGWCG	3 cut(s)	152, 323, 1475
HpyAV	CCTTC	8 cut(s)	21, 85, 430, 813, 975, 1091, 1252, 1309
HpyCH4III	ACNGT	4 cut(s)	276, 284, 494, 853
HpyCH4IV	ACGT	1 cut(s)	713
HpyF10VI	GCNNNNNNNGC	7 cut(s)	170, 225, 515, 682, 941, 950, 1540
HpyF3I	CTNAG	3 cut(s)	82, 209, 1125
HpySE526I	ACGT	1 cut(s)	713
Hsp92I	GRCGYC	1 cut(s)	150
Hsp92II	CATG	7 cut(s)	797, 888, 1087, 1291, 1348, 1516, 1571
KspAI	GTTAAC	1 cut(s)	406
KspI	CCGCGG	1 cut(s)	677
Kzo9I	GATC	5 cut(s)	577, 1063, 1242, 1467, 1570
LmnI	GCTCC	5 cut(s)	104, 612, 676, 1165, 1292
Lsp1109I	GCAGC	2 cut(s)	108, 713
LweI	GCATC	5 cut(s)	694, 777, 787, 899, 1129
MaeI	CTAG	2 cut(s)	1002, 1049
MaeII	ACGT	1 cut(s)	713
MaeIII	GTNAC	8 cut(s)	113, 250, 284, 473, 559, 856, 901, 1267
MalI	GATC	5 cut(s)	579, 1065, 1244, 1469, 1572
MboI	GATC	5 cut(s)	577, 1063, 1242, 1467, 1570
MboII	GAAGA	8 cut(s)	190, 401, 844, 847, 1058, 1252, 1292, 1565
MfeI	CAATTG	1 cut(s)	59
MflI	RGATCY	2 cut(s)	1063, 1242
MhlI	GDGCHC	1 cut(s)	1584
MlsI	TGGCCA	1 cut(s)	592
MluCI	AATT	7 cut(s)	59, 237, 290, 533, 619, 694, 1610
MluNI	TGGCCA	1 cut(s)	592
MlyI	GAGTC	1 cut(s)	951
MmeI	TCCRAC	4 cut(s)	330, 343, 555, 1497
Mox20I	TGGCCA	1 cut(s)	592
Mph1103I	ATGCAT	3 cut(s)	6, 1144, 1289
MroXI	GAANNNNTTC	1 cut(s)	1438
MscI	TGGCCA	1 cut(s)	592
MseI	TTAA	6 cut(s)	261, 405, 513, 525, 597, 866
MslI	CAYNNNNRTG	1 cut(s)	773
Msp20I	TGGCCA	1 cut(s)	592
MspA1I	CMGCKG	3 cut(s)	676, 784, 1073
MspI	CCGG	9 cut(s)	38, 102, 174, 480, 758, 1247, 1320, 1325, 1386
MspR9I	CCNGG	6 cut(s)	26, 175, 610, 1247, 1253, 1326
MunI	CAATTG	1 cut(s)	59
Mva1269I	GAATGC	1 cut(s)	1289
MvaI	CCWGG	3 cut(s)	26, 610, 1253
MvnI	CGCG	3 cut(s)	225, 318, 676
MwoI	GCNNNNNNNGC	7 cut(s)	170, 225, 515, 682, 941, 950, 1540
NciI	CCSGG	3 cut(s)	175, 1247, 1326
NdeI	CATATG	2 cut(s)	1190, 1411
NdeII	GATC	5 cut(s)	577, 1063, 1242, 1467, 1570
NlaIII	CATG	7 cut(s)	797, 888, 1087, 1291, 1348, 1516, 1571
NlaIV	GGNNCC	4 cut(s)	30, 278, 1107, 1167
NmuCI	GTSAC	5 cut(s)	113, 250, 284, 473, 856
NsiI	ATGCAT	3 cut(s)	6, 1144, 1289
NspI	RCATGY	1 cut(s)	797
PagI	TCATGA	1 cut(s)	1567
PceI	AGGCCT	1 cut(s)	1356
PciI	ACATGT	1 cut(s)	793
PctI	GAATGC	1 cut(s)	1289
PdmI	GAANNNNTTC	1 cut(s)	1438
PfeI	GAWTC	2 cut(s)	1156, 1547
PkrI	GCNGC	8 cut(s)	98, 227, 485, 488, 520, 678, 703, 999
PleI	GAGTC	1 cut(s)	951
PpsI	GAGTC	1 cut(s)	951
PpuMI	RGGWCCY	2 cut(s)	1106, 1180
PscI	ACATGT	1 cut(s)	793
Psp124BI	GAGCTC	1 cut(s)	1584
Psp5II	RGGWCCY	2 cut(s)	1106, 1180
Psp6I	CCWGG	3 cut(s)	24, 608, 1251
PspGI	CCWGG	3 cut(s)	24, 608, 1251
PspN4I	GGNNCC	4 cut(s)	30, 278, 1107, 1167
PspPI	GGNCC	7 cut(s)	86, 638, 1106, 1180, 1249, 1322, 1487
PspPPI	RGGWCCY	2 cut(s)	1106, 1180
PsuI	RGATCY	2 cut(s)	1063, 1242
RsaI	GTAC	5 cut(s)	798, 871, 1407, 1605, 1618
RsaNI	GTAC	5 cut(s)	797, 870, 1406, 1604, 1617
RseI	CAYNNNNRTG	1 cut(s)	773
Rsr2I	CGGWCCG	1 cut(s)	1322
RsrII	CGGWCCG	1 cut(s)	1322
SacI	GAGCTC	1 cut(s)	1584
SacII	CCGCGG	1 cut(s)	677
SaqAI	TTAA	6 cut(s)	261, 405, 513, 525, 597, 866
SatI	GCNGC	8 cut(s)	97, 226, 484, 487, 519, 677, 702, 998
Sau3AI	GATC	5 cut(s)	577, 1063, 1242, 1467, 1570
Sau96I	GGNCC	7 cut(s)	86, 638, 1106, 1180, 1249, 1322, 1487
ScaI	AGTACT	1 cut(s)	871
SchI	GAGTC	1 cut(s)	951
ScrFI	CCNGG	6 cut(s)	26, 175, 610, 1247, 1253, 1326
SduI	GDGCHC	1 cut(s)	1584
SfaNI	GCATC	5 cut(s)	694, 777, 787, 899, 1129
SfcI	CTRYAG	2 cut(s)	1119, 1170
Sfr303I	CCGCGG	1 cut(s)	677
SgrAI	CRCCGGYG	1 cut(s)	479
SgrBI	CCGCGG	1 cut(s)	677
SinI	GGWCC	5 cut(s)	1106, 1180, 1249, 1322, 1487
SmiMI	CAYNNNNRTG	1 cut(s)	773
SmlI	CTYRAG	2 cut(s)	214, 811
SmoI	CTYRAG	2 cut(s)	214, 811
Sse9I	AATT	7 cut(s)	59, 237, 290, 533, 619, 694, 1610
SseBI	AGGCCT	1 cut(s)	1356
SspI	AATATT	1 cut(s)	1439
SspMI	CTAG	2 cut(s)	1002, 1049
SstI	GAGCTC	1 cut(s)	1584
StuI	AGGCCT	1 cut(s)	1356
StyD4I	CCNGG	6 cut(s)	24, 173, 608, 1245, 1251, 1324
StyI	CCWWGG	2 cut(s)	186, 587
TaaI	ACNGT	4 cut(s)	276, 284, 494, 853
TaiI	ACGT	1 cut(s)	716
TaqI	TCGA	2 cut(s)	147, 1113
TasI	AATT	7 cut(s)	59, 237, 290, 533, 619, 694, 1610
TatI	WGTACW	2 cut(s)	869, 1616
TauI	GCSGC	6 cut(s)	228, 486, 489, 521, 679, 1000
TfiI	GAWTC	2 cut(s)	1156, 1547
Tru1I	TTAA	6 cut(s)	261, 405, 513, 525, 597, 866
Tru9I	TTAA	6 cut(s)	261, 405, 513, 525, 597, 866
TscAI	CASTG	4 cut(s)	213, 287, 778, 1533
TseFI	GTSAC	5 cut(s)	113, 250, 284, 473, 856
TseI	GCWGC	2 cut(s)	96, 701
Tsp45I	GTSAC	5 cut(s)	113, 250, 284, 473, 856
TspDTI	ATGAA	4 cut(s)	558, 761, 881, 1556
TspGWI	ACGGA	4 cut(s)	269, 348, 875, 1540
TspRI	CASTG	4 cut(s)	213, 287, 778, 1533
VpaK11BI	GGWCC	5 cut(s)	1106, 1180, 1249, 1322, 1487
XapI	RAATTY	3 cut(s)	237, 290, 533
XceI	RCATGY	1 cut(s)	797
XmiI	GTMKAC	1 cut(s)	42
XmnI	GAANNNNTTC	1 cut(s)	1438
XspI	CTAG	2 cut(s)	1002, 1049
ZrmI	AGTACT	1 cut(s)	871
Zsp2I	ATGCAT	3 cut(s)	6, 1144, 1289

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.