Rh6AG397400

Monogalactosyldiacylglycerol synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0011941
Physical Location & Seq
Forward (+)
59627645 .. 59630210
2566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG397400.1

Sequence Viewer

Length: 1086 bp
ATGTCTCAGGTGTTTGTTACAGACTTATGGTCGGATCATACGCCTTGGCCATTTAATCAACTTCCCAGGAGCTATAATTTCTTGGTGAAACATGGACCATTGTGGAAAATGACATATTATGGAACTGCTCCACGCCTGGTGCATCAATCTAATTTTGCTGCGACTTCTACATTTATAGCTCGGGAGGTTGCCAAAGGACTGATGAAATACCAGCCAGATATTATTATCAGTGTGCATCCGCTGATGCAACACGTACCACTTCGTGTACTGAGGCAAAAGGGTCTGTTGGAGAAGATAGTTTTTACTACAGTCATCACAGATTTAAGCACTTGTCATCCTACATGGTTTCATAAGCTTGTAACAAGATGTTATTGTCCGACATCTGAGGTTGCGAAAAGGGCATCGAAAGCCGGACTCAAGCCTTCACAAATCAAGGTTTATGGCCTCCCTGTGCGGCCTTCTTTTGTTAAGCCTGTGCGACCAAAGGCTGAGCTTCGGAGAGAATTAGGAATGGAAGAGCATCTTCCTGCTGTGTTGCTGATGGGAGGAGGAGAAGGGATGGGTCCTATTGAGGCTACAGCCCGAGCACTTGGGGATGCATTATATGATGAAAATCTTGGGGAGCCTATGGGTCAGGTCCTTGTGGTATGTGGCCGCAACAAAAAGCTTGCAGAAAAGTTGCGCGCAATCGATTGGAAGGTTCCTGTCCAGGTGAAGGGATTTGTCACTAAAATGGAGGAAGGCATGGGAGCTTGTGATTGCATAATTACAAAGGCAGGCCCAGGGACTATTGCAGAAGCAATGATAAGAGGCCTCCCAATTATTCTGAATGATTACATTGCCGGGCAGGAAGTTGGAAATGTCCCATATGTTGTCGAAAACGGGTGTGGGAAATATTCTAAATCTCCAAAAGAGATAGCAAGGATTGTTTCAGAGTGGTTTGGTCCAAAAGCTGATGAGCTGCAGGCGATGTCGCAAAACGCATTGAAGCTGGCAAGGCCTGACTCTGTGTTCAAGATTGTTCATGATATGCACGAGCTTGTTCGACAACGAAGCTTTGTACCCCAGTACTCATGTACAACCTAA

Protein Analysis

361

Amino Acids

40.26

Weight (kDa)

9.27

Isoelectric Point (pI)

42.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MGDG_synth PF06925 2 - 151 1.2e-54 Monogalactosyldiacylglycerol (MGDG) synthase
Glyco_tran_28_C PF04101 208 - 318 9e-09 Glycosyltransferase family 28 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011941)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31780 AT4G31780 AT4G31780
fragaria_vesca FvH4_2g41310
malus_domestica MD08G1052400.v1.1 MD15G1043100.v1.1
prunus_persica Prupe.1G396500_v2.0.a1
pyrus_communis pycom08g04370 pycom15g04010
rosa_chinensis RchiOBHm_Chr6g0299211
rosa_laevigata RLG00000011453
rosa_roxburghii Rroxscaffold_7G00168910
rosa_rugosa Rorug06G0286300 Rorug06G0286300
rosa_samantha Rh6AG397400 Rh6BG406000 Rh6CG412100 Rh6DG398800
rosa_wichuraiana Rw6G034790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 684
AciI CCGC 3 cut(s) 239, 454, 655
AclWI GGATC 1 cut(s) 42
AcoI YGGCCR 2 cut(s) 47, 652
AdeI CACNNNGTG 1 cut(s) 263
AfaI GTAC 5 cut(s) 255, 267, 1062, 1070, 1078
AfiI CCNNNNNNNGG 1 cut(s) 715
AflIII ACRYGT 1 cut(s) 250
AgsI TTSAA 2 cut(s) 988, 1015
AhdI GACNNNNNGTC 1 cut(s) 28
AjnI CCWGG 4 cut(s) 65, 135, 708, 781
Alw21I GWGCWC 1 cut(s) 589
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 42
Ama87I CYCGRG 2 cut(s) 180, 582
AoxI GGCC 7 cut(s) 47, 442, 455, 652, 778, 811, 998
ApeKI GCWGC 2 cut(s) 158, 961
AspLEI GCGC 2 cut(s) 684, 686
AspS9I GGNCC 5 cut(s) 95, 563, 637, 779, 944
AsuC2I CCSGG 1 cut(s) 844
AsuHPI GGTGA 2 cut(s) 97, 724
AvaI CYCGRG 2 cut(s) 180, 582
AvaII GGWCC 4 cut(s) 95, 563, 637, 944
BalI TGGCCA 1 cut(s) 49
BauI CACGAG 1 cut(s) 1034
Bbv12I GWGCWC 1 cut(s) 589
BbvI GCAGC 2 cut(s) 145, 948
BccI CCATC 2 cut(s) 535, 553
BciT130I CCWGG 4 cut(s) 67, 137, 710, 783
BcnI CCSGG 1 cut(s) 844
BcoDI GTCTC 1 cut(s) 9
BfmI CTRYAG 3 cut(s) 306, 576, 962
BisI GCNGC 4 cut(s) 159, 455, 655, 962
BlpI GCTNAGC 1 cut(s) 489
BlsI GCNGC 4 cut(s) 160, 456, 656, 963
BmcAI AGTACT 1 cut(s) 1070
Bme1390I CCNGG 5 cut(s) 67, 137, 710, 783, 844
Bme18I GGWCC 4 cut(s) 95, 563, 637, 944
BmeRI GACNNNNNGTC 1 cut(s) 28
BmeT110I CYCGRG 2 cut(s) 180, 582
BmgT120I GGNCC 5 cut(s) 95, 563, 637, 779, 944
BmiI GGNNCC 3 cut(s) 564, 624, 702
BmrFI CCNGG 5 cut(s) 67, 137, 710, 783, 844
BmrI ACTGGG 1 cut(s) 1060
BmsI GCATC 6 cut(s) 151, 234, 244, 410, 529, 586
BmuI ACTGGG 1 cut(s) 1060
Bpu1102I GCTNAGC 1 cut(s) 489
BpuEI CTTGAG 1 cut(s) 401
BpuMI CCSGG 1 cut(s) 844
Bsa29I ATCGAT 1 cut(s) 690
BsaAI YACGTR 1 cut(s) 253
BsaBI GATNNNNATC 1 cut(s) 612
BsaJI CCNNGG 4 cut(s) 44, 65, 781, 782
Bsc4I CCNNNNNNNGG 1 cut(s) 715
Bse1I ACTGG 1 cut(s) 1066
Bse3DI GCAATG 2 cut(s) 807, 837
Bse8I GATNNNNATC 1 cut(s) 612
BseBI CCWGG 4 cut(s) 67, 137, 710, 783
BseCI ATCGAT 1 cut(s) 690
BseDI CCNNGG 4 cut(s) 44, 65, 781, 782
BseGI GGATG 4 cut(s) 235, 334, 564, 601
BseJI GATNNNNATC 1 cut(s) 612
BseLI CCNNNNNNNGG 1 cut(s) 715
BseMI GCAATG 2 cut(s) 807, 837
BseMII CTCAG 4 cut(s) 20, 260, 375, 480
BseNI ACTGG 1 cut(s) 1066
BsePI GCGCGC 1 cut(s) 682
BseRI GAGGAG 2 cut(s) 561, 564
BseXI GCAGC 2 cut(s) 145, 948
Bsh1236I CGCG 1 cut(s) 684
BshFI GGCC 7 cut(s) 49, 444, 457, 654, 780, 813, 1000
BshVI ATCGAT 1 cut(s) 690
BsiHKAI GWGCWC 1 cut(s) 589
BsiHKCI CYCGRG 2 cut(s) 180, 582
BsiSI CCGG 2 cut(s) 411, 843
BslFI GGGAC 2 cut(s) 799, 848
BslI CCNNNNNNNGG 1 cut(s) 715
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 799, 848
BsnI GGCC 7 cut(s) 49, 444, 457, 654, 780, 813, 1000
BsoBI CYCGRG 2 cut(s) 180, 582
Bsp1286I GDGCHC 1 cut(s) 589
Bsp1407I TGTACA 1 cut(s) 1076
Bsp143I GATC 1 cut(s) 34
Bsp1720I GCTNAGC 1 cut(s) 489
BspACI CCGC 3 cut(s) 239, 454, 655
BspANI GGCC 7 cut(s) 49, 444, 457, 654, 780, 813, 1000
BspCNI CTCAG 4 cut(s) 19, 261, 376, 481
BspDI ATCGAT 1 cut(s) 690
BspFNI CGCG 1 cut(s) 684
BspHI TCATGA 1 cut(s) 1024
BspLI GGNNCC 3 cut(s) 564, 624, 702
BspMAI CTGCAG 1 cut(s) 966
BspPI GGATC 1 cut(s) 42
BspQI GCTCTTC 1 cut(s) 510
BsrDI GCAATG 2 cut(s) 807, 837
BsrGI TGTACA 1 cut(s) 1076
BsrI ACTGG 1 cut(s) 1066
BssECI CCNNGG 4 cut(s) 44, 65, 781, 782
BssHII GCGCGC 1 cut(s) 682
BssMI GATC 1 cut(s) 34
BssSI CACGAG 1 cut(s) 1034
BssT1I CCWWGG 1 cut(s) 44
Bst2BI CACGAG 1 cut(s) 1034
Bst2UI CCWGG 4 cut(s) 67, 137, 710, 783
Bst4CI ACNGT 1 cut(s) 310
Bst6I CTCTTC 1 cut(s) 510
BstAUI TGTACA 1 cut(s) 1076
BstBAI YACGTR 1 cut(s) 253
BstC8I GCNNGC 5 cut(s) 669, 684, 778, 966, 993
BstDEI CTNAG 4 cut(s) 6, 269, 384, 489
BstF5I GGATG 4 cut(s) 235, 334, 564, 601
BstFNI CGCG 1 cut(s) 684
BstHHI GCGC 2 cut(s) 684, 686
BstKTI GATC 1 cut(s) 37
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 3 cut(s) 398, 407, 997
BstNI CCWGG 4 cut(s) 67, 137, 710, 783
BstSCI CCNGG 5 cut(s) 65, 135, 708, 781, 842
BstSFI CTRYAG 3 cut(s) 306, 576, 962
BstUI CGCG 1 cut(s) 684
BstV1I GCAGC 2 cut(s) 145, 948
Bsu15I ATCGAT 1 cut(s) 690
BsuRI GGCC 7 cut(s) 49, 444, 457, 654, 780, 813, 1000
BsuTUI ATCGAT 1 cut(s) 690
BtgZI GCGATG 1 cut(s) 983
BtsCI GGATG 4 cut(s) 235, 334, 564, 601
BtsIMutI CAGTG 1 cut(s) 235
Cac8I GCNNGC 5 cut(s) 669, 684, 778, 966, 993
CciI TCATGA 1 cut(s) 1024
CfoI GCGC 2 cut(s) 684, 686
Cfr13I GGNCC 5 cut(s) 95, 563, 637, 779, 944
ClaI ATCGAT 1 cut(s) 690
Csp6I GTAC 5 cut(s) 254, 266, 1061, 1069, 1077
CviAII CATG 5 cut(s) 92, 342, 745, 1025, 1074
CviQI GTAC 5 cut(s) 254, 266, 1061, 1069, 1077
DdeI CTNAG 4 cut(s) 6, 269, 384, 489
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
DraIII CACNNNGTG 1 cut(s) 263
DriI GACNNNNNGTC 1 cut(s) 28
EaeI YGGCCR 2 cut(s) 47, 652
Eam1104I CTCTTC 1 cut(s) 510
Eam1105I GACNNNNNGTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 510
Eco130I CCWWGG 1 cut(s) 44
Eco147I AGGCCT 2 cut(s) 813, 1000
Eco47I GGWCC 4 cut(s) 95, 563, 637, 944
Eco88I CYCGRG 2 cut(s) 180, 582
EcoO109I RGGNCCY 2 cut(s) 563, 637
EcoRII CCWGG 4 cut(s) 65, 135, 708, 781
EcoT14I CCWWGG 1 cut(s) 44
EcoT22I ATGCAT 1 cut(s) 601
ErhI CCWWGG 1 cut(s) 44
FaeI CATG 5 cut(s) 95, 345, 748, 1028, 1077
FalI AAGNNNNNCTT 2 cut(s) 507, 539
FaqI GGGAC 2 cut(s) 799, 848
FatI CATG 5 cut(s) 91, 341, 744, 1024, 1073
FauNDI CATATG 1 cut(s) 868
Fnu4HI GCNGC 4 cut(s) 159, 455, 655, 962
FokI GGATG 4 cut(s) 222, 321, 571, 608
Fsp4HI GCNGC 4 cut(s) 159, 455, 655, 962
GlaI GCGC 2 cut(s) 683, 685
GluI GCNGC 4 cut(s) 159, 455, 655, 962
HaeIII GGCC 7 cut(s) 49, 444, 457, 654, 780, 813, 1000
HapII CCGG 2 cut(s) 411, 843
HhaI GCGC 2 cut(s) 684, 686
Hin1II CATG 5 cut(s) 95, 345, 748, 1028, 1077
Hin6I GCGC 2 cut(s) 682, 684
HinP1I GCGC 2 cut(s) 682, 684
HindIII AAGCTT 3 cut(s) 353, 665, 1054
HinfI GANTC 2 cut(s) 414, 1004
HpaII CCGG 2 cut(s) 411, 843
HphI GGTGA 2 cut(s) 97, 724
Hpy166II GTNNAC 1 cut(s) 266
Hpy188I TCNGA 6 cut(s) 34, 378, 385, 498, 828, 934
Hpy188III TCNNGA 3 cut(s) 182, 1015, 1025
Hpy8I GTNNAC 1 cut(s) 266
HpyAV CCTTC 6 cut(s) 432, 468, 548, 691, 709, 734
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 9 cut(s) 142, 235, 247, 599, 671, 762, 794, 964, 1033
HpyF10VI GCNNNNNNNGC 3 cut(s) 398, 407, 997
HpyF3I CTNAG 4 cut(s) 6, 269, 384, 489
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 5 cut(s) 95, 345, 748, 1028, 1077
HspAI GCGC 2 cut(s) 682, 684
Kzo9I GATC 1 cut(s) 34
LguI GCTCTTC 1 cut(s) 510
LmnI GCTCC 4 cut(s) 69, 133, 622, 749
Lsp1109I GCAGC 2 cut(s) 145, 948
LweI GCATC 6 cut(s) 151, 234, 244, 410, 529, 586
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 3 cut(s) 16, 358, 724
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MboII GAAGA 3 cut(s) 304, 515, 527
MhlI GDGCHC 1 cut(s) 589
MlsI TGGCCA 1 cut(s) 49
MluCI AATT 5 cut(s) 76, 151, 503, 765, 819
MluNI TGGCCA 1 cut(s) 49
MlyI GAGTC 2 cut(s) 408, 998
MmeI TCCRAC 4 cut(s) 12, 267, 401, 835
Mox20I TGGCCA 1 cut(s) 49
Mph1103I ATGCAT 1 cut(s) 601
MscI TGGCCA 1 cut(s) 49
MseI TTAA 3 cut(s) 54, 323, 468
MslI CAYNNNNRTG 1 cut(s) 731
Msp20I TGGCCA 1 cut(s) 49
MspA1I CMGCKG 1 cut(s) 241
MspI CCGG 2 cut(s) 411, 843
MspR9I CCNGG 5 cut(s) 67, 137, 710, 783, 844
MvaI CCWGG 4 cut(s) 67, 137, 710, 783
MvnI CGCG 1 cut(s) 684
MwoI GCNNNNNNNGC 3 cut(s) 398, 407, 997
NciI CCSGG 1 cut(s) 844
NdeI CATATG 1 cut(s) 868
NdeII GATC 1 cut(s) 34
NlaIII CATG 5 cut(s) 95, 345, 748, 1028, 1077
NlaIV GGNNCC 3 cut(s) 564, 624, 702
NmuCI GTSAC 1 cut(s) 724
NsiI ATGCAT 1 cut(s) 601
PagI TCATGA 1 cut(s) 1024
PasI CCCWGGG 1 cut(s) 782
PauI GCGCGC 1 cut(s) 682
PceI AGGCCT 2 cut(s) 813, 1000
PciSI GCTCTTC 1 cut(s) 510
PkrI GCNGC 4 cut(s) 160, 456, 656, 963
PleI GAGTC 2 cut(s) 408, 998
PpsI GAGTC 2 cut(s) 408, 998
Ppu21I YACGTR 1 cut(s) 253
PpuMI RGGWCCY 2 cut(s) 563, 637
Psp5II RGGWCCY 2 cut(s) 563, 637
Psp6I CCWGG 4 cut(s) 65, 135, 708, 781
PspGI CCWGG 4 cut(s) 65, 135, 708, 781
PspN4I GGNNCC 3 cut(s) 564, 624, 702
PspPI GGNCC 5 cut(s) 95, 563, 637, 779, 944
PspPPI RGGWCCY 2 cut(s) 563, 637
PstI CTGCAG 1 cut(s) 966
PteI GCGCGC 1 cut(s) 682
RsaI GTAC 5 cut(s) 255, 267, 1062, 1070, 1078
RsaNI GTAC 5 cut(s) 254, 266, 1061, 1069, 1077
RseI CAYNNNNRTG 1 cut(s) 731
SapI GCTCTTC 1 cut(s) 510
SaqAI TTAA 3 cut(s) 54, 323, 468
SatI GCNGC 4 cut(s) 159, 455, 655, 962
Sau3AI GATC 1 cut(s) 34
Sau96I GGNCC 5 cut(s) 95, 563, 637, 779, 944
ScaI AGTACT 1 cut(s) 1070
SchI GAGTC 2 cut(s) 408, 998
ScrFI CCNGG 5 cut(s) 67, 137, 710, 783, 844
SduI GDGCHC 1 cut(s) 589
SfaNI GCATC 6 cut(s) 151, 234, 244, 410, 529, 586
SfcI CTRYAG 3 cut(s) 306, 576, 962
SinI GGWCC 4 cut(s) 95, 563, 637, 944
SmiMI CAYNNNNRTG 1 cut(s) 731
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
Sse9I AATT 5 cut(s) 76, 151, 503, 765, 819
SseBI AGGCCT 2 cut(s) 813, 1000
SsiI CCGC 3 cut(s) 239, 454, 655
SspI AATATT 1 cut(s) 896
StuI AGGCCT 2 cut(s) 813, 1000
StyD4I CCNGG 5 cut(s) 65, 135, 708, 781, 842
StyI CCWWGG 1 cut(s) 44
TaaI ACNGT 1 cut(s) 310
TaiI ACGT 1 cut(s) 255
TaqI TCGA 4 cut(s) 404, 690, 876, 1045
TasI AATT 5 cut(s) 76, 151, 503, 765, 819
TatI WGTACW 3 cut(s) 265, 1068, 1076
TauI GCSGC 2 cut(s) 457, 657
Tru1I TTAA 3 cut(s) 54, 323, 468
Tru9I TTAA 3 cut(s) 54, 323, 468
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 1 cut(s) 724
TseI GCWGC 2 cut(s) 158, 961
Tsp45I GTSAC 1 cut(s) 724
TspDTI ATGAA 4 cut(s) 218, 338, 624, 1013
TspRI CASTG 1 cut(s) 235
VpaK11BI GGWCC 4 cut(s) 95, 563, 637, 944
ZrmI AGTACT 1 cut(s) 1070
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.