Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0011941

Physical Location & Seq

Genomic Coordinates Reverse (-)

9526080 .. 9530220

Gene Structure 4141 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000011453

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGCATAATCCTTCGATAACCACCACCCAGGAACCCAGTCCTCTCTATGACCTTGTGTCCCAATTGGGTCGGTTTGCCTTGAGCACAAGGTTTCGAAACCTGCGCTCTGAGGGCCACTCCGCCCTTTTATCTAATTTCCTCTACTTTGATGGGCCCAGAAACACCAAGGTCAGTGCTTCTCTCAGTTTGAACACTAGAGGCAGCGGCTCCAGCATTCGGAGAATTGTCACCGAGTTCAATAGAGCTATTAGGTTCCATTGTGACAAATTCCCTATTGGCTTCGCTTCTCTTGGCGTTGGTGGTGGGGATAGCAATGGATCAGATAATGGTGCCGGTGCCGCCGACAACAATAATGGATTGAGAGACAATGTCTGCACTCTTAACGACAATGGAGTAGCCTTGAATAGTGCGGTGGCAGGCAGCGCCAAAAAAGTGCTGATTTTGATGAGTGACACCGGTGGCGGTCATAGAGCTTCAGCAGAAGCTATTAAGGCGGCCTTTAATGAAGAATTCGGAGATGAATATCAGGTGTTTGTTACAGACTTATGGTCGGATCATACGCCTTGGCCATTTAATCAACTTCCCAGGAGCTATAATTTCTTGGTGAAACATGGACCATTATGGAAAATGACATATTATGGAACTGCTCCACGCCTGGTGCATCAATCTAATTTTGCTGCGACTTCAACATTTATAGCTCGGGAGGTTGCCAAAGGACTGATGAAATACCAGCCAGATATTATTATCAGTGTGCATCCGCTGATGCAACACGTACCACTTCGTGTACTGAGGCAAAAGGGTCTGTTGGAGAAGATAGTTTTTACTACAGTCATCACAGATTTAAGCACTTGTCATCCTACATGGTTTCATAAGCTTGTAACAAGATGTTATTGTCCGACATCTGAGGTTGCAAAAAGGGCATCGAAAGCCGGACTCAAGCCTTCGCAAATCAAGGTTTATGGCCTCCCCGTGCGGCCTTCTTTTGTTAAGCCTGTGCGACCAAAGGCTGAGCTTCGGAGAGAATTAGGAATGGAAGAGCATCTTCCGGCCGTGTTGCTGATGGGAGGAGGAGAAGGGATGGGTCCTATTGAGGCTACAGCCCGAGCACTTGGGGATGCATTATATGATGAAAATCTTGGGGAGCCTATGGGTCAGGTCCTTGTGGTATGTGGCCACAACAAAAAGCTTGCAGAAAAGTTGCGCACAATCGATTGGAAGGTTCCTGTCCAGGTGAAGGGATTTGTCACTAAAATGGAGGAAGGCATGGGAGCTTGTGATTGCATAATTACAAAAGCGGGCCCAGGGACTATTGCAGAAGCAATGATAAGAGGCCTCCCAATTATTCTGAATGATTACATTGCCGGGCAGGAAGTTGGAAATGTCCCATATGTTGTCGAAAATGGGTGTGGGAAATATTCTAAATCTCCAAAAGAGATAGCAAGGATTGTTTCAGAGTGGTTTGGTCCAAAAGCTGATGAGCTCCAGGCGATGTCGCAAAACGCATTGAAGCTGGCAAGGCCTGATTCTGTGTTCAAGATTGTTCATGATATGCACGAGCTTGTTCGACAACGAAGCTTTGTACCCCAGTACTCATATGATCAGAGCTATGTCCGTGGCTGGCTGAAGATTTCACCTCAGACAAGTACAGTGGCTGAAGATTTCATCTCCGGTTCAGTTTGGGGTGAACCCGGAGATGACCTCACGGAACCTGGAAACCTGAATCCTACTCTTCCCATTTTGATAAGCTCTTCTCGTCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006643 GO:0006664 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0008378 GO:0008610 GO:0009058 GO:0009247 GO:0009507 GO:0009526 GO:0009528 GO:0009536 GO:0009657 GO:0009668 GO:0009706 GO:0009790 GO:0009791 GO:0009793 GO:0009941 GO:0009987 GO:0010027 GO:0010154 GO:0016020 GO:0016043 GO:0016740 GO:0016757 GO:0016758 GO:0019866 GO:0022414 GO:0031090 GO:0031967 GO:0031969 GO:0031975 GO:0032501 GO:0032502 GO:0035250 GO:0042170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046467 GO:0046509 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061024 GO:0061458 GO:0071704 GO:0071840 GO:1901135 GO:1901137 GO:1901576 GO:1903509

KEGG Pathways

Metabolic & Signaling

ko00561 ko01100 map00561 map01100

Pfam Domains

Protein Families

Glyco_tran_28_C MGDG_synth

Protein Analysis

587

Amino Acids

64.27

Weight (kDa)

8.77

Isoelectric Point (pI)

41.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MGDG_synth	PF06925	156 - 324	6.3e-63	Monogalactosyldiacylglycerol (MGDG) synthase
Glyco_tran_28_C	PF04101	381 - 472	2.1e-08	Glycosyltransferase family 28 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011941)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31780 AT4G31780 AT4G31780
fragaria_vesca	FvH4_2g41310
malus_domestica	MD08G1052400.v1.1 MD15G1043100.v1.1
prunus_persica	Prupe.1G396500_v2.0.a1
pyrus_communis	pycom08g04370 pycom15g04010
rosa_chinensis	RchiOBHm_Chr6g0299211
rosa_laevigata	RLG00000011453
rosa_roxburghii	Rroxscaffold_7G00168910
rosa_rugosa	Rorug06G0286300 Rorug06G0286300
rosa_samantha	Rh6AG397400 Rh6BG406000 Rh6CG412100 Rh6DG398800
rosa_wichuraiana	Rw6G034790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1202
Acc36I	ACCTGC	1 cut(s)	108
AccB1I	GGYRCC	2 cut(s)	329, 335
AciI	CCGC	9 cut(s)	120, 204, 339, 410, 462, 494, 758, 973, 1295
AclWI	GGATC	2 cut(s)	325, 561
AcoI	YGGCCR	3 cut(s)	566, 1047, 1171
AcsI	RAATTY	2 cut(s)	266, 509
AcuI	CTGAAG	3 cut(s)	459, 1643, 1674
AdeI	CACNNNGTG	1 cut(s)	782
AfaI	GTAC	5 cut(s)	774, 786, 1581, 1589, 1645
AfiI	CCNNNNNNNGG	2 cut(s)	216, 1234
AflIII	ACRYGT	1 cut(s)	769
AgeI	ACCGGT	1 cut(s)	455
AgsI	TTSAA	6 cut(s)	190, 238, 403, 687, 1507, 1534
AhdI	GACNNNNNGTC	2 cut(s)	55, 547
AjnI	CCWGG	7 cut(s)	27, 584, 654, 1227, 1300, 1482, 1708
Alw21I	GWGCWC	3 cut(s)	86, 1108, 1482
Alw26I	GTCTC	1 cut(s)	357
AlwI	GGATC	2 cut(s)	325, 561
AlwNI	CAGNNNCTG	1 cut(s)	1652
Ama87I	CYCGRG	2 cut(s)	699, 1101
ApaI	GGGCCC	2 cut(s)	156, 1301
ApeKI	GCWGC	3 cut(s)	201, 420, 677
ApoI	RAATTY	2 cut(s)	266, 509
AsiGI	ACCGGT	1 cut(s)	455
AspLEI	GCGC	3 cut(s)	105, 425, 1203
AspS9I	GGNCC	9 cut(s)	112, 152, 153, 614, 1082, 1156, 1297, 1298, 1463
AsuC2I	CCSGG	2 cut(s)	1363, 1689
AsuHPI	GGTGA	5 cut(s)	220, 616, 1243, 1623, 1694
AsuII	TTCGAA	1 cut(s)	94
AvaI	CYCGRG	2 cut(s)	699, 1101
AvaII	GGWCC	4 cut(s)	614, 1082, 1156, 1463
BaeGI	GKGCMC	2 cut(s)	156, 1301
BalI	TGGCCA	2 cut(s)	568, 1173
BanI	GGYRCC	2 cut(s)	329, 335
BanII	GRGCYC	3 cut(s)	156, 1301, 1482
BauI	CACGAG	1 cut(s)	1553
Bbv12I	GWGCWC	3 cut(s)	86, 1108, 1482
BbvI	GCAGC	3 cut(s)	213, 432, 664
BccI	CCATC	3 cut(s)	143, 1054, 1072
BceAI	ACGGC	1 cut(s)	1034
BciT130I	CCWGG	7 cut(s)	29, 586, 656, 1229, 1302, 1484, 1710
BclI	TGATCA	1 cut(s)	1597
BcnI	CCSGG	2 cut(s)	1363, 1689
BcoDI	GTCTC	1 cut(s)	357
BfaI	CTAG	1 cut(s)	195
BfmI	CTRYAG	2 cut(s)	825, 1095
BfoI	RGCGCY	1 cut(s)	426
BfuAI	ACCTGC	1 cut(s)	108
BisI	GCNGC	7 cut(s)	202, 205, 339, 421, 495, 678, 974
BlpI	GCTNAGC	1 cut(s)	1008
BlsI	GCNGC	7 cut(s)	203, 206, 340, 422, 496, 679, 975
BmcAI	AGTACT	1 cut(s)	1589
Bme1390I	CCNGG	9 cut(s)	29, 586, 656, 1229, 1302, 1363, 1484, 1689, 1710
Bme18I	GGWCC	4 cut(s)	614, 1082, 1156, 1463
BmeRI	GACNNNNNGTC	2 cut(s)	55, 547
BmeT110I	CYCGRG	2 cut(s)	699, 1101
BmgT120I	GGNCC	9 cut(s)	112, 152, 153, 614, 1082, 1156, 1297, 1298, 1463
BmrFI	CCNGG	9 cut(s)	29, 586, 656, 1229, 1302, 1363, 1484, 1689, 1710
BmrI	ACTGGG	2 cut(s)	30, 1579
BmsI	GCATC	6 cut(s)	670, 753, 763, 929, 1048, 1105
BmuI	ACTGGG	2 cut(s)	30, 1579
BplI	GAGNNNNNCTC	4 cut(s)	101, 133, 1683, 1715
BpmI	CTGGAG	2 cut(s)	193, 1466
Bpu1102I	GCTNAGC	1 cut(s)	1008
Bpu14I	TTCGAA	1 cut(s)	94
BpuEI	CTTGAG	2 cut(s)	100, 920
BpuMI	CCSGG	2 cut(s)	1363, 1689
Bsa29I	ATCGAT	1 cut(s)	1209
BsaAI	YACGTR	1 cut(s)	772
BsaBI	GATNNNNATC	2 cut(s)	522, 1131
BsaJI	CCNNGG	7 cut(s)	27, 165, 563, 584, 1300, 1301, 1612
BsaWI	WCCGGW	2 cut(s)	455, 1667
Bsc4I	CCNNNNNNNGG	2 cut(s)	216, 1234
Bse118I	RCCGGY	2 cut(s)	332, 455
Bse1I	ACTGG	2 cut(s)	36, 1585
Bse3DI	GCAATG	3 cut(s)	319, 1326, 1356
Bse8I	GATNNNNATC	2 cut(s)	522, 1131
BseBI	CCWGG	7 cut(s)	29, 586, 656, 1229, 1302, 1484, 1710
BseCI	ATCGAT	1 cut(s)	1209
BseDI	CCNNGG	7 cut(s)	27, 165, 563, 584, 1300, 1301, 1612
BseGI	GGATG	4 cut(s)	754, 853, 1083, 1120
BseJI	GATNNNNATC	2 cut(s)	522, 1131
BseLI	CCNNNNNNNGG	2 cut(s)	216, 1234
BseMI	GCAATG	3 cut(s)	319, 1326, 1356
BseMII	CTCAG	6 cut(s)	99, 196, 779, 894, 999, 1649
BseNI	ACTGG	2 cut(s)	36, 1585
BseRI	GAGGAG	2 cut(s)	1080, 1083
BseSI	GKGCMC	2 cut(s)	156, 1301
BseX3I	CGGCCG	1 cut(s)	1047
BseXI	GCAGC	3 cut(s)	213, 432, 664
BsgI	GTGCAG	1 cut(s)	358
Bsh1285I	CGRYCG	1 cut(s)	1050
BshNI	GGYRCC	2 cut(s)	329, 335
BshTI	ACCGGT	1 cut(s)	455
BshVI	ATCGAT	1 cut(s)	1209
BsiEI	CGRYCG	1 cut(s)	1050
BsiHKAI	GWGCWC	3 cut(s)	86, 1108, 1482
BsiHKCI	CYCGRG	2 cut(s)	699, 1101
BsiSI	CCGG	7 cut(s)	333, 456, 930, 1046, 1362, 1668, 1689
BslFI	GGGAC	3 cut(s)	43, 1318, 1367
BslI	CCNNNNNNNGG	2 cut(s)	216, 1234
BsmAI	GTCTC	1 cut(s)	357
BsmFI	GGGAC	3 cut(s)	43, 1318, 1367
BsmI	GAATGC	1 cut(s)	213
BsoBI	CYCGRG	2 cut(s)	699, 1101
Bsp119I	TTCGAA	1 cut(s)	94
Bsp120I	GGGCCC	2 cut(s)	152, 1297
Bsp1286I	GDGCHC	5 cut(s)	86, 156, 1108, 1301, 1482
Bsp143I	GATC	3 cut(s)	317, 553, 1597
Bsp1720I	GCTNAGC	1 cut(s)	1008
BspACI	CCGC	9 cut(s)	120, 204, 339, 410, 462, 494, 758, 973, 1295
BspCNI	CTCAG	6 cut(s)	100, 195, 780, 895, 1000, 1648
BspDI	ATCGAT	1 cut(s)	1209
BspHI	TCATGA	1 cut(s)	1543
BspMI	ACCTGC	1 cut(s)	108
BspPI	GGATC	2 cut(s)	325, 561
BspQI	GCTCTTC	2 cut(s)	1029, 1753
BspT104I	TTCGAA	1 cut(s)	94
BspT107I	GGYRCC	2 cut(s)	329, 335
BsrDI	GCAATG	3 cut(s)	319, 1326, 1356
BsrFI	RCCGGY	2 cut(s)	332, 455
BsrI	ACTGG	2 cut(s)	36, 1585
BssAI	RCCGGY	2 cut(s)	332, 455
BssECI	CCNNGG	7 cut(s)	27, 165, 563, 584, 1300, 1301, 1612
BssMI	GATC	3 cut(s)	317, 553, 1597
BssSI	CACGAG	1 cut(s)	1553
BssT1I	CCWWGG	2 cut(s)	165, 563
Bst2BI	CACGAG	1 cut(s)	1553
Bst2UI	CCWGG	7 cut(s)	29, 586, 656, 1229, 1302, 1484, 1710
Bst4CI	ACNGT	2 cut(s)	829, 1648
Bst6I	CTCTTC	3 cut(s)	1029, 1734, 1753
BstBAI	YACGTR	1 cut(s)	772
BstBI	TTCGAA	1 cut(s)	94
BstC8I	GCNNGC	5 cut(s)	418, 1188, 1297, 1512, 1619
BstDEI	CTNAG	6 cut(s)	108, 182, 788, 903, 1008, 1635
BstDSI	CCRYGG	1 cut(s)	1612
BstF5I	GGATG	4 cut(s)	754, 853, 1083, 1120
BstH2I	RGCGCY	1 cut(s)	426
BstHHI	GCGC	3 cut(s)	105, 425, 1203
BstKTI	GATC	3 cut(s)	320, 556, 1600
BstMAI	GTCTC	1 cut(s)	357
BstMBI	GATC	3 cut(s)	317, 553, 1597
BstMCI	CGRYCG	1 cut(s)	1050
BstMWI	GCNNNNNNNGC	8 cut(s)	111, 210, 338, 422, 491, 917, 926, 1516
BstNI	CCWGG	7 cut(s)	29, 586, 656, 1229, 1302, 1484, 1710
BstSCI	CCNGG	9 cut(s)	27, 584, 654, 1227, 1300, 1361, 1482, 1687, 1708
BstSFI	CTRYAG	2 cut(s)	825, 1095
BstSLI	GKGCMC	2 cut(s)	156, 1301
BstV1I	GCAGC	3 cut(s)	213, 432, 664
BstZI	CGGCCG	1 cut(s)	1047
Bsu15I	ATCGAT	1 cut(s)	1209
BsuTUI	ATCGAT	1 cut(s)	1209
BtgI	CCRYGG	1 cut(s)	1612
BtgZI	GCGATG	1 cut(s)	1502
BtsCI	GGATG	4 cut(s)	754, 853, 1083, 1120
BtsIMutI	CAGTG	3 cut(s)	178, 754, 1653
BveI	ACCTGC	1 cut(s)	108
Cac8I	GCNNGC	5 cut(s)	418, 1188, 1297, 1512, 1619
CaiI	CAGNNNCTG	1 cut(s)	1652
CciI	TCATGA	1 cut(s)	1543
CfoI	GCGC	3 cut(s)	105, 425, 1203
Cfr10I	RCCGGY	2 cut(s)	332, 455
Cfr13I	GGNCC	9 cut(s)	112, 152, 153, 614, 1082, 1156, 1297, 1298, 1463
ClaI	ATCGAT	1 cut(s)	1209
Csp6I	GTAC	5 cut(s)	773, 785, 1580, 1588, 1644
CspAI	ACCGGT	1 cut(s)	455
CspCI	CAANNNNNGTGG	2 cut(s)	1629, 1664
CviAII	CATG	4 cut(s)	611, 861, 1264, 1544
CviQI	GTAC	5 cut(s)	773, 785, 1580, 1588, 1644
DdeI	CTNAG	6 cut(s)	108, 182, 788, 903, 1008, 1635
DpnI	GATC	3 cut(s)	319, 555, 1599
DpnII	GATC	3 cut(s)	317, 553, 1597
DraIII	CACNNNGTG	1 cut(s)	782
DriI	GACNNNNNGTC	2 cut(s)	55, 547
EaeI	YGGCCR	3 cut(s)	566, 1047, 1171
EagI	CGGCCG	1 cut(s)	1047
Eam1104I	CTCTTC	3 cut(s)	1029, 1734, 1753
Eam1105I	GACNNNNNGTC	2 cut(s)	55, 547
EarI	CTCTTC	3 cut(s)	1029, 1734, 1753
EciI	GGCGGA	1 cut(s)	109
Ecl136II	GAGCTC	1 cut(s)	1480
EclXI	CGGCCG	1 cut(s)	1047
Eco130I	CCWWGG	2 cut(s)	165, 563
Eco147I	AGGCCT	2 cut(s)	1332, 1519
Eco24I	GRGCYC	3 cut(s)	156, 1301, 1482
Eco47I	GGWCC	4 cut(s)	614, 1082, 1156, 1463
Eco52I	CGGCCG	1 cut(s)	1047
Eco53kI	GAGCTC	1 cut(s)	1480
Eco57I	CTGAAG	3 cut(s)	459, 1643, 1674
Eco88I	CYCGRG	2 cut(s)	699, 1101
EcoICRI	GAGCTC	1 cut(s)	1480
EcoO109I	RGGNCCY	2 cut(s)	1082, 1156
EcoRI	GAATTC	1 cut(s)	509
EcoRII	CCWGG	7 cut(s)	27, 584, 654, 1227, 1300, 1482, 1708
EcoT14I	CCWWGG	2 cut(s)	165, 563
EcoT22I	ATGCAT	2 cut(s)	6, 1120
EcoT38I	GRGCYC	3 cut(s)	156, 1301, 1482
ErhI	CCWWGG	2 cut(s)	165, 563
FaeI	CATG	4 cut(s)	614, 864, 1267, 1547
FalI	AAGNNNNNCTT	4 cut(s)	482, 514, 1026, 1058
FaqI	GGGAC	3 cut(s)	43, 1318, 1367
FatI	CATG	4 cut(s)	610, 860, 1263, 1543
FauI	CCCGC	1 cut(s)	1288
FauNDI	CATATG	2 cut(s)	1387, 1594
FbaI	TGATCA	1 cut(s)	1597
Fnu4HI	GCNGC	7 cut(s)	202, 205, 339, 421, 495, 678, 974
FokI	GGATG	4 cut(s)	741, 840, 1090, 1127
FriOI	GRGCYC	3 cut(s)	156, 1301, 1482
Fsp4HI	GCNGC	7 cut(s)	202, 205, 339, 421, 495, 678, 974
FspBI	CTAG	1 cut(s)	195
FspI	TGCGCA	1 cut(s)	1202
GlaI	GCGC	3 cut(s)	104, 424, 1202
GluI	GCNGC	7 cut(s)	202, 205, 339, 421, 495, 678, 974
GsuI	CTGGAG	2 cut(s)	193, 1466
HaeII	RGCGCY	1 cut(s)	426
HapII	CCGG	7 cut(s)	333, 456, 930, 1046, 1362, 1668, 1689
HhaI	GCGC	3 cut(s)	105, 425, 1203
Hin1II	CATG	4 cut(s)	614, 864, 1267, 1547
Hin6I	GCGC	3 cut(s)	103, 423, 1201
HinP1I	GCGC	3 cut(s)	103, 423, 1201
HindIII	AAGCTT	3 cut(s)	872, 1184, 1573
HinfI	GANTC	3 cut(s)	933, 1523, 1720
HpaII	CCGG	7 cut(s)	333, 456, 930, 1046, 1362, 1668, 1689
HphI	GGTGA	5 cut(s)	220, 616, 1243, 1623, 1694
Hpy166II	GTNNAC	2 cut(s)	785, 1685
Hpy188III	TCNNGA	3 cut(s)	701, 1534, 1544
Hpy8I	GTNNAC	2 cut(s)	785, 1685
HpyAV	CCTTC	7 cut(s)	21, 951, 987, 1067, 1210, 1228, 1253
HpyCH4III	ACNGT	2 cut(s)	829, 1648
HpyCH4IV	ACGT	1 cut(s)	771
HpyF10VI	GCNNNNNNNGC	8 cut(s)	111, 210, 338, 422, 491, 917, 926, 1516
HpyF3I	CTNAG	6 cut(s)	108, 182, 788, 903, 1008, 1635
HpySE526I	ACGT	1 cut(s)	771
Hsp92II	CATG	4 cut(s)	614, 864, 1267, 1547
HspAI	GCGC	3 cut(s)	103, 423, 1201
Ksp22I	TGATCA	1 cut(s)	1597
Kzo9I	GATC	3 cut(s)	317, 553, 1597
LguI	GCTCTTC	2 cut(s)	1029, 1753
LmnI	GCTCC	6 cut(s)	212, 588, 652, 1141, 1268, 1485
Lsp1109I	GCAGC	3 cut(s)	213, 432, 664
LweI	GCATC	6 cut(s)	670, 753, 763, 929, 1048, 1105
MaeI	CTAG	1 cut(s)	195
MaeII	ACGT	1 cut(s)	771
MaeIII	GTNAC	6 cut(s)	226, 260, 449, 535, 877, 1243
MalI	GATC	3 cut(s)	319, 555, 1599
MboI	GATC	3 cut(s)	317, 553, 1597
MboII	GAAGA	8 cut(s)	518, 823, 1034, 1046, 1636, 1667, 1721, 1740
MfeI	CAATTG	1 cut(s)	62
MhlI	GDGCHC	5 cut(s)	86, 156, 1108, 1301, 1482
MlsI	TGGCCA	2 cut(s)	568, 1173
MluNI	TGGCCA	2 cut(s)	568, 1173
MlyI	GAGTC	1 cut(s)	927
MmeI	TCCRAC	4 cut(s)	531, 786, 920, 1354
Mox20I	TGGCCA	2 cut(s)	568, 1173
Mph1103I	ATGCAT	2 cut(s)	6, 1120
MscI	TGGCCA	2 cut(s)	568, 1173
MseI	TTAA	7 cut(s)	381, 489, 501, 573, 842, 987, 1759
MslI	CAYNNNNRTG	1 cut(s)	1250
Msp20I	TGGCCA	2 cut(s)	568, 1173
MspA1I	CMGCKG	2 cut(s)	204, 760
MspI	CCGG	7 cut(s)	333, 456, 930, 1046, 1362, 1668, 1689
MspR9I	CCNGG	9 cut(s)	29, 586, 656, 1229, 1302, 1363, 1484, 1689, 1710
MunI	CAATTG	1 cut(s)	62
Mva1269I	GAATGC	1 cut(s)	213
MvaI	CCWGG	7 cut(s)	29, 586, 656, 1229, 1302, 1484, 1710
MwoI	GCNNNNNNNGC	8 cut(s)	111, 210, 338, 422, 491, 917, 926, 1516
NciI	CCSGG	2 cut(s)	1363, 1689
NdeI	CATATG	2 cut(s)	1387, 1594
NdeII	GATC	3 cut(s)	317, 553, 1597
NlaIII	CATG	4 cut(s)	614, 864, 1267, 1547
NmuCI	GTSAC	4 cut(s)	226, 260, 449, 1243
NsbI	TGCGCA	1 cut(s)	1202
NsiI	ATGCAT	2 cut(s)	6, 1120
NspV	TTCGAA	1 cut(s)	94
PagI	TCATGA	1 cut(s)	1543
PasI	CCCWGGG	1 cut(s)	1301
PceI	AGGCCT	2 cut(s)	1332, 1519
PciSI	GCTCTTC	2 cut(s)	1029, 1753
PctI	GAATGC	1 cut(s)	213
PfeI	GAWTC	2 cut(s)	1523, 1720
PflFI	GACNNNGTC	1 cut(s)	368
PinAI	ACCGGT	1 cut(s)	455
PkrI	GCNGC	7 cut(s)	203, 206, 340, 422, 496, 679, 975
PleI	GAGTC	1 cut(s)	927
PpsI	GAGTC	1 cut(s)	927
Ppu21I	YACGTR	1 cut(s)	772
PpuMI	RGGWCCY	2 cut(s)	1082, 1156
Psp124BI	GAGCTC	1 cut(s)	1482
Psp5II	RGGWCCY	2 cut(s)	1082, 1156
Psp6I	CCWGG	7 cut(s)	27, 584, 654, 1227, 1300, 1482, 1708
PspGI	CCWGG	7 cut(s)	27, 584, 654, 1227, 1300, 1482, 1708
PspOMI	GGGCCC	2 cut(s)	152, 1297
PspPI	GGNCC	9 cut(s)	112, 152, 153, 614, 1082, 1156, 1297, 1298, 1463
PspPPI	RGGWCCY	2 cut(s)	1082, 1156
PstNI	CAGNNNCTG	1 cut(s)	1652
PsyI	GACNNNGTC	1 cut(s)	368
RsaI	GTAC	5 cut(s)	774, 786, 1581, 1589, 1645
RsaNI	GTAC	5 cut(s)	773, 785, 1580, 1588, 1644
RseI	CAYNNNNRTG	1 cut(s)	1250
SacI	GAGCTC	1 cut(s)	1482
SapI	GCTCTTC	2 cut(s)	1029, 1753
SaqAI	TTAA	7 cut(s)	381, 489, 501, 573, 842, 987, 1759
SatI	GCNGC	7 cut(s)	202, 205, 339, 421, 495, 678, 974
Sau3AI	GATC	3 cut(s)	317, 553, 1597
Sau96I	GGNCC	9 cut(s)	112, 152, 153, 614, 1082, 1156, 1297, 1298, 1463
ScaI	AGTACT	1 cut(s)	1589
SchI	GAGTC	1 cut(s)	927
ScrFI	CCNGG	9 cut(s)	29, 586, 656, 1229, 1302, 1363, 1484, 1689, 1710
SduI	GDGCHC	5 cut(s)	86, 156, 1108, 1301, 1482
SfaNI	GCATC	6 cut(s)	670, 753, 763, 929, 1048, 1105
SfcI	CTRYAG	2 cut(s)	825, 1095
SfuI	TTCGAA	1 cut(s)	94
SgrAI	CRCCGGYG	1 cut(s)	455
SinI	GGWCC	4 cut(s)	614, 1082, 1156, 1463
SmiMI	CAYNNNNRTG	1 cut(s)	1250
SmlI	CTYRAG	2 cut(s)	79, 935
SmoI	CTYRAG	2 cut(s)	79, 935
SseBI	AGGCCT	2 cut(s)	1332, 1519
SsiI	CCGC	9 cut(s)	120, 204, 339, 410, 462, 494, 758, 973, 1295
SspI	AATATT	1 cut(s)	1415
SspMI	CTAG	1 cut(s)	195
SstI	GAGCTC	1 cut(s)	1482
StuI	AGGCCT	2 cut(s)	1332, 1519
StyD4I	CCNGG	9 cut(s)	27, 584, 654, 1227, 1300, 1361, 1482, 1687, 1708
StyI	CCWWGG	2 cut(s)	165, 563
TaaI	ACNGT	2 cut(s)	829, 1648
TaiI	ACGT	1 cut(s)	774
TaqI	TCGA	6 cut(s)	14, 94, 923, 1209, 1395, 1564
TatI	WGTACW	3 cut(s)	784, 1587, 1643
TauI	GCSGC	4 cut(s)	207, 341, 497, 976
TfiI	GAWTC	2 cut(s)	1523, 1720
Tru1I	TTAA	7 cut(s)	381, 489, 501, 573, 842, 987, 1759
Tru9I	TTAA	7 cut(s)	381, 489, 501, 573, 842, 987, 1759
TscAI	CASTG	3 cut(s)	178, 754, 1653
TseFI	GTSAC	4 cut(s)	226, 260, 449, 1243
TseI	GCWGC	3 cut(s)	201, 420, 677
Tsp45I	GTSAC	4 cut(s)	226, 260, 449, 1243
TspDTI	ATGAA	7 cut(s)	519, 534, 737, 857, 1143, 1532, 1651
TspGWI	ACGGA	2 cut(s)	1601, 1718
TspRI	CASTG	3 cut(s)	178, 754, 1653
Tth111I	GACNNNGTC	1 cut(s)	368
VpaK11BI	GGWCC	4 cut(s)	614, 1082, 1156, 1463
XapI	RAATTY	2 cut(s)	266, 509
XspI	CTAG	1 cut(s)	195
ZrmI	AGTACT	1 cut(s)	1589
Zsp2I	ATGCAT	2 cut(s)	6, 1120

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.