Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008207

Physical Location & Seq

Genomic Coordinates Reverse (-)

4370752 .. 4373974

Gene Structure 3223 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1056300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1923 bp

ATGGCATCTCGTAGATCTCCGGCAGTCCTGGCGATCTCTGTGATCCTCTTACTTCTCGTCCATGGCGCCCACTCCTTCTACCTCCCCGGCGTCGCTCCGGAGGACTTCATCAAGGGAGATGATTTGAAAGTTAAAGTGAACAAATTGACTTCTACAAAGACTCAGCTTCCATACTCATATTACTCTCTCCGTTACTGCAAACCTGATAAAATCTTGGACAGTACAGAGAATCTTGGGGAAGTGCTCCGCGGTGACCGGATCGAGAACTCCCCCTATGTGTTTAAAATGCGGGAGCCTCAGATGTGCAACATTGTCTGCCGGTTAACGCCTGATGCCAAAATTGTAAAGCAGTTTAAGGAGAAGATAGATGATGAGTATCGAGTGAACATGATCCTTGATAATCTACCTCTAGTTTTTCCCATTCAAAGGCCAGACCAAGACGCTCCTACAGTTTATCAGCTTGGATTCCACGTTGGGCTCAAAGGCCATTATGCTGGGAGCAAAGAAGAGAAATACTTCATCCACAACCATCTGGCATTTACTGTGAAGTACCATAGAGATTCGCAAACTGAAACAGCAAGAATCGTGGGATTTGAGGTTAAACCATTCAGTGTTAAACATGAATATGAAGGGAAATGGAATGATGCAAAGACTCGTTTAACTACCTGTGACCCCCATTCGAAACACACTGTTGTTAATTCTAACTCTCCTCAAGAGGTTGCCGAAAAGCAAGAAATCGTATTTACCTATGATGTTGAATTCCAGGAGAGTGATGTGAAGTGGGCTTCTAGATGGGATGCTTATCTACTGATGAGTGACGACCAAATCCATTGGTTTTCAATTGTCAACTCATTGATGATTGTCCTCTTCCTTTCGGGTATGGTAGCGATGATTATGCTAAGAACACTCTACCGTGATATCTCCAAGTATAATGAACTTGAGACCCAGGAAGAAGCGCAAGAAGAGACAGGATGGAAACTTGTCCACGGGGATGTTTTCCGGGCTCCAAATAACTCTGAATTGCTCTGCGTATATGTTGGAACTGGTGTTCAGTTCTTTGGAATGATACTGGTGACAATGATTTTTGCTGTCCTTGGCTTCCTCTCCCCATCTAACCGGGGCGGTCTTATGACTGCAATGCTCTTGCTTTTTGTCTTCATGGGAATTTTTGCTGGTTACGCCTCTGTTCGCTTGTACAAGATGTTTAAAGGTACAGAATGGAAGAAAATTGCATTCAGAACTGCTGTCATGTTCCCAGCAACTGTCTCTGCCATTTTCATTGTCTTGAACACTCTGATTTGGGGCCAGAAATCATCTGGAGCTGTGCCATTTGGCACAATGTTTGCCCTGGTCTTTTTATGGTTCGGAATCTCAGTCCCACTCGTATTTGTGGGAGGCTATGTTGGGTTCAGGAAGCCAGCACTTGAGGACCCAGTGAAGACAAACAAAATCCCAAGACAGATCCCAGAACAGGCCTGGTACATGAACCCAGTTTTCTCAATTCTGATTGGAGGGATCCTCCCATTCGGAGCAGTTTTCATCGAACTATTCTTCATCCTCACTTCAATTTGGCTGAACCAGTTCTACTACATCTTTGGTTTCCTCTTCTTGGTCTTCATCATCCTCATCATCACATGCGCTGAGATCACCATCGTCCTCTGCTACTTCCAGTTGTGCAGCGAGGATTACTTGTGGTGGTGGAGATCATACCTCACATCTGGCTCATCGGCTCTGTACCTCTTCCTCTACGCCACATTCTACTTCTTCACAAAGCTTGAAATCACAAAGCTGGTCTCGGCCCTCTTATACTTCGGATACATGCTCATTGCATCGTATGCATTCTTTGTTGTCACCGGAACCATCGGCTTCTACGCCTGCTTTTGGTTCACGAGGCTCATCTACTCGTCGGTGAAGATCGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0006810 GO:0006873 GO:0006885 GO:0006897 GO:0006909 GO:0006950 GO:0007155 GO:0008150 GO:0009505 GO:0009628 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016192 GO:0019725 GO:0022607 GO:0022610 GO:0030003 GO:0030004 GO:0030312 GO:0030641 GO:0031090 GO:0031224 GO:0031410 GO:0031982 GO:0031984 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033157 GO:0034622 GO:0036293 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0048878 GO:0050789 GO:0050801 GO:0050896 GO:0051049 GO:0051050 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0060341 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0070201 GO:0070482 GO:0070861 GO:0070863 GO:0071840 GO:0071944 GO:0090087 GO:0090316 GO:0097708 GO:0098588 GO:0098657 GO:0098771 GO:0098791 GO:0098805 GO:1903827 GO:1903829 GO:1904951 GO:2000008 GO:2000010

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EMP70

Protein Analysis

641

Amino Acids

73.53

Weight (kDa)

7.92

Isoelectric Point (pI)

40.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EMP70	PF02990	57 - 596	3.2e-221	Endomembrane protein 70

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10840 AT5G25100 AT5G25100
fragaria_vesca	FvH4_2g41140
malus_domestica	MD08G1056300.v1.1 MD08G1056400.v1.1 MD08G1056900.v1.1 MD15G1040700.v1.1
prunus_persica	Prupe.1G393900_v2.0.a1
pyrus_communis	pycom08g04620 pycom08g04630 pycom15g03800
rosa_chinensis	RchiOBHm_Chr6g0299401
rosa_multiflora	Rmu_sc0000861.1_g000066 Rmu_sc0005509.1_g000001 Rmu_sc0013965.1_g000004 Rmu_sc0017526.1_g000004
rosa_roxburghii	Rroxscaffold_7G00168720
rosa_rugosa	Rorug06G0287800
rosa_samantha	Rh6BG407900 Rh6CG413700 Rh6DG400700
rosa_wichuraiana	Rw6G034980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	65
AccII	CGCG	1 cut(s)	249
AccIII	TCCGGA	1 cut(s)	97
AciI	CCGC	4 cut(s)	247, 249, 289, 1122
AclWI	GGATC	6 cut(s)	37, 266, 385, 1456, 1510, 1523
AcsI	RAATTY	2 cut(s)	758, 1164
AcyI	GRCGYC	2 cut(s)	66, 90
AfaI	GTAC	6 cut(s)	223, 551, 1196, 1213, 1481, 1736
AfiI	CCNNNNNNNGG	4 cut(s)	426, 1471, 1609, 1881
AgsI	TTSAA	7 cut(s)	127, 425, 758, 840, 1288, 1566, 1778
AjnI	CCWGG	5 cut(s)	27, 762, 945, 1347, 1475
AloI	GAACNNNNNNTCC	2 cut(s)	1032, 1064
AluBI	AGCT	5 cut(s)	166, 460, 1322, 1774, 1789
AluI	AGCT	5 cut(s)	166, 460, 1322, 1774, 1789
Alw21I	GWGCWC	1 cut(s)	246
Alw26I	GTCTC	4 cut(s)	935, 959, 1270, 1798
AlwI	GGATC	6 cut(s)	37, 266, 385, 1456, 1510, 1523
AlwNI	CAGNNNCTG	1 cut(s)	1262
Aor13HI	TCCGGA	1 cut(s)	97
AoxI	GGCC	5 cut(s)	428, 484, 1303, 1473, 1797
ApeKI	GCWGC	1 cut(s)	1677
ApoI	RAATTY	2 cut(s)	758, 1164
Asp700I	GAANNNNTTC	2 cut(s)	515, 1580
AspLEI	GCGC	3 cut(s)	68, 958, 1640
AspS9I	GGNCC	3 cut(s)	1303, 1429, 1798
AsuC2I	CCSGG	3 cut(s)	87, 1001, 1118
AsuHPI	GGTGA	5 cut(s)	263, 1084, 1639, 1843, 1921
AsuII	TTCGAA	1 cut(s)	680
AvaII	GGWCC	1 cut(s)	1429
BamHI	GGATCC	1 cut(s)	1515
BanI	GGYRCC	1 cut(s)	65
BanII	GRGCYC	2 cut(s)	480, 1006
BauI	CACGAG	1 cut(s)	1888
BbsI	GAAGAC	3 cut(s)	1147, 1445, 1606
Bbv12I	GWGCWC	1 cut(s)	246
BbvI	GCAGC	1 cut(s)	1689
BccI	CCATC	6 cut(s)	537, 786, 966, 1117, 1658, 1868
BcgI	CGANNNNNNTGC	2 cut(s)	877, 911
BciT130I	CCWGG	5 cut(s)	29, 764, 947, 1349, 1477
BciVI	GTATCC	1 cut(s)	1808
BcnI	CCSGG	3 cut(s)	87, 1001, 1118
BcoDI	GTCTC	4 cut(s)	935, 959, 1270, 1798
BfaI	CTAG	2 cut(s)	410, 789
BfmI	CTRYAG	1 cut(s)	447
BfoI	RGCGCY	1 cut(s)	69
BfuI	GTATCC	1 cut(s)	1808
BglII	AGATCT	1 cut(s)	14
BisI	GCNGC	1 cut(s)	1678
BlsI	GCNGC	1 cut(s)	1679
Bme1390I	CCNGG	8 cut(s)	29, 87, 764, 947, 1001, 1118, 1349, 1477
Bme18I	GGWCC	1 cut(s)	1429
BmgT120I	GGNCC	3 cut(s)	1303, 1429, 1798
BmiI	GGNNCC	7 cut(s)	67, 294, 1005, 1304, 1431, 1517, 1858
BmrFI	CCNGG	8 cut(s)	29, 87, 764, 947, 1001, 1118, 1349, 1477
BmrI	ACTGGG	2 cut(s)	1427, 1484
BmsI	GCATC	5 cut(s)	14, 322, 634, 787, 1838
BmuI	ACTGGG	2 cut(s)	1427, 1484
BpiI	GAAGAC	3 cut(s)	1147, 1445, 1606
BplI	GAGNNNNNCTC	2 cut(s)	1503, 1535
BpmI	CTGGAG	1 cut(s)	1338
Bpu14I	TTCGAA	1 cut(s)	680
BpuEI	CTTGAG	3 cut(s)	696, 959, 1445
BpuMI	CCSGG	3 cut(s)	87, 1001, 1118
BsaBI	GATNNNNATC	3 cut(s)	375, 801, 1649
BsaHI	GRCGYC	2 cut(s)	66, 90
BsaI	GGTCTC	2 cut(s)	935, 1798
BsaJI	CCNNGG	8 cut(s)	61, 85, 247, 945, 985, 1093, 1117, 1347
BsaWI	WCCGGW	3 cut(s)	97, 255, 1853
BsaXI	ACNNNNNCTCC	2 cut(s)	1386, 1416
Bsc4I	CCNNNNNNNGG	4 cut(s)	426, 1471, 1609, 1881
Bse118I	RCCGGY	1 cut(s)	318
Bse1I	ACTGG	6 cut(s)	1048, 1074, 1433, 1490, 1579, 1669
Bse3DI	GCAATG	2 cut(s)	1143, 1824
Bse8I	GATNNNNATC	3 cut(s)	375, 801, 1649
BseAI	TCCGGA	1 cut(s)	97
BseBI	CCWGG	5 cut(s)	29, 764, 947, 1349, 1477
BseDI	CCNNGG	8 cut(s)	61, 85, 247, 945, 985, 1093, 1117, 1347
BseGI	GGATG	6 cut(s)	519, 802, 977, 997, 1554, 1620
BseJI	GATNNNNATC	3 cut(s)	375, 801, 1649
BseLI	CCNNNNNNNGG	4 cut(s)	426, 1471, 1609, 1881
BseMI	GCAATG	2 cut(s)	1143, 1824
BseMII	CTCAG	4 cut(s)	176, 311, 1386, 1632
BseNI	ACTGG	6 cut(s)	1048, 1074, 1433, 1490, 1579, 1669
BseRI	GAGGAG	1 cut(s)	699
BseXI	GCAGC	1 cut(s)	1689
BseYI	CCCAGC	2 cut(s)	494, 1255
BsgI	GTGCAG	1 cut(s)	1696
Bsh1236I	CGCG	1 cut(s)	249
BshFI	GGCC	5 cut(s)	430, 486, 1305, 1475, 1799
BshNI	GGYRCC	1 cut(s)	65
BsiHKAI	GWGCWC	1 cut(s)	246
BsiSI	CCGG	8 cut(s)	20, 87, 98, 256, 319, 1000, 1117, 1854
BslFI	GGGAC	1 cut(s)	1361
BslI	CCNNNNNNNGG	4 cut(s)	426, 1471, 1609, 1881
BsmAI	GTCTC	4 cut(s)	935, 959, 1270, 1798
BsmFI	GGGAC	1 cut(s)	1361
BsmI	GAATGC	2 cut(s)	1232, 1838
BsnI	GGCC	5 cut(s)	430, 486, 1305, 1475, 1799
Bso31I	GGTCTC	2 cut(s)	935, 1798
Bsp119I	TTCGAA	1 cut(s)	680
Bsp1286I	GDGCHC	3 cut(s)	246, 480, 1006
Bsp13I	TCCGGA	1 cut(s)	97
Bsp1407I	TGTACA	1 cut(s)	1194
Bsp19I	CCATGG	1 cut(s)	61
BspACI	CCGC	4 cut(s)	247, 249, 289, 1122
BspANI	GGCC	5 cut(s)	430, 486, 1305, 1475, 1799
BspCNI	CTCAG	4 cut(s)	175, 310, 1385, 1633
BspEI	TCCGGA	1 cut(s)	97
BspFNI	CGCG	1 cut(s)	249
BspLI	GGNNCC	7 cut(s)	67, 294, 1005, 1304, 1431, 1517, 1858
BspPI	GGATC	6 cut(s)	37, 266, 385, 1456, 1510, 1523
BspT104I	TTCGAA	1 cut(s)	680
BspT107I	GGYRCC	1 cut(s)	65
BspTNI	GGTCTC	2 cut(s)	935, 1798
BsrDI	GCAATG	2 cut(s)	1143, 1824
BsrFI	RCCGGY	1 cut(s)	318
BsrGI	TGTACA	1 cut(s)	1194
BsrI	ACTGG	6 cut(s)	1048, 1074, 1433, 1490, 1579, 1669
BssAI	RCCGGY	1 cut(s)	318
BssECI	CCNNGG	8 cut(s)	61, 85, 247, 945, 985, 1093, 1117, 1347
BssNI	GRCGYC	2 cut(s)	66, 90
BssSI	CACGAG	1 cut(s)	1888
BssT1I	CCWWGG	2 cut(s)	61, 1093
Bst2BI	CACGAG	1 cut(s)	1888
Bst2UI	CCWGG	5 cut(s)	29, 764, 947, 1349, 1477
Bst4CI	ACNGT	6 cut(s)	221, 451, 544, 691, 914, 1264
Bst6I	CTCTTC	5 cut(s)	501, 872, 957, 1610, 1745
BstACI	GRCGYC	2 cut(s)	66, 90
BstAPI	GCANNNNNTGC	1 cut(s)	1835
BstAUI	TGTACA	1 cut(s)	1194
BstBI	TTCGAA	1 cut(s)	680
BstC8I	GCNNGC	2 cut(s)	1419, 1876
BstDEI	CTNAG	5 cut(s)	162, 297, 899, 1372, 1641
BstDSI	CCRYGG	3 cut(s)	61, 247, 985
BstEII	GGTNACC	1 cut(s)	251
BstF5I	GGATG	6 cut(s)	519, 802, 977, 997, 1554, 1620
BstFNI	CGCG	1 cut(s)	249
BstH2I	RGCGCY	1 cut(s)	69
BstHHI	GCGC	3 cut(s)	68, 958, 1640
BstMAI	GTCTC	4 cut(s)	935, 959, 1270, 1798
BstMWI	GCNNNNNNNGC	3 cut(s)	29, 1178, 1835
BstNI	CCWGG	5 cut(s)	29, 764, 947, 1349, 1477
BstNSI	RCATGY	2 cut(s)	1638, 1822
BstPI	GGTNACC	1 cut(s)	251
BstSCI	CCNGG	8 cut(s)	27, 85, 762, 945, 999, 1116, 1347, 1475
BstSFI	CTRYAG	1 cut(s)	447
BstUI	CGCG	1 cut(s)	249
BstV1I	GCAGC	1 cut(s)	1689
BstV2I	GAAGAC	3 cut(s)	1147, 1445, 1606
BstX2I	RGATCY	3 cut(s)	14, 1461, 1515
BstXI	CCANNNNNNTGG	1 cut(s)	494
BstYI	RGATCY	3 cut(s)	14, 1461, 1515
BsuI	GTATCC	1 cut(s)	1808
BsuRI	GGCC	5 cut(s)	430, 486, 1305, 1475, 1799
BtgI	CCRYGG	3 cut(s)	61, 247, 985
BtgZI	GCGATG	1 cut(s)	902
BtsCI	GGATG	6 cut(s)	519, 802, 977, 997, 1554, 1620
BtsIMutI	CAGTG	3 cut(s)	616, 687, 1440
Cac8I	GCNNGC	2 cut(s)	1419, 1876
CaiI	CAGNNNCTG	1 cut(s)	1262
CfoI	GCGC	3 cut(s)	68, 958, 1640
Cfr10I	RCCGGY	1 cut(s)	318
Cfr13I	GGNCC	3 cut(s)	1303, 1429, 1798
Cfr42I	CCGCGG	1 cut(s)	250
CseI	GACGC	2 cut(s)	79, 449
Csp6I	GTAC	6 cut(s)	222, 550, 1195, 1212, 1480, 1735
CspCI	CAANNNNNGTGG	2 cut(s)	567, 602
CviAII	CATG	8 cut(s)	62, 388, 620, 1159, 1249, 1483, 1635, 1819
CviQI	GTAC	6 cut(s)	222, 550, 1195, 1212, 1480, 1735
DdeI	CTNAG	5 cut(s)	162, 297, 899, 1372, 1641
DinI	GGCGCC	1 cut(s)	67
DraI	TTTAAA	2 cut(s)	283, 1207
Eam1104I	CTCTTC	5 cut(s)	501, 872, 957, 1610, 1745
EarI	CTCTTC	5 cut(s)	501, 872, 957, 1610, 1745
Eco130I	CCWWGG	2 cut(s)	61, 1093
Eco147I	AGGCCT	1 cut(s)	1475
Eco24I	GRGCYC	2 cut(s)	480, 1006
Eco31I	GGTCTC	2 cut(s)	935, 1798
Eco32I	GATATC	1 cut(s)	919
Eco47I	GGWCC	1 cut(s)	1429
Eco91I	GGTNACC	1 cut(s)	251
EcoO109I	RGGNCCY	1 cut(s)	1429
EcoO65I	GGTNACC	1 cut(s)	251
EcoRI	GAATTC	1 cut(s)	758
EcoRII	CCWGG	5 cut(s)	27, 762, 945, 1347, 1475
EcoRV	GATATC	1 cut(s)	919
EcoT14I	CCWWGG	2 cut(s)	61, 1093
EcoT22I	ATGCAT	1 cut(s)	1840
EcoT38I	GRGCYC	2 cut(s)	480, 1006
EgeI	GGCGCC	1 cut(s)	67
EheI	GGCGCC	1 cut(s)	67
ErhI	CCWWGG	2 cut(s)	61, 1093
FaeI	CATG	8 cut(s)	65, 391, 623, 1162, 1252, 1486, 1638, 1822
FaqI	GGGAC	1 cut(s)	1361
FatI	CATG	8 cut(s)	61, 387, 619, 1158, 1248, 1482, 1634, 1818
FauI	CCCGC	1 cut(s)	282
Fnu4HI	GCNGC	1 cut(s)	1678
FokI	GGATG	6 cut(s)	506, 809, 984, 1004, 1541, 1607
FriOI	GRGCYC	2 cut(s)	480, 1006
Fsp4HI	GCNGC	1 cut(s)	1678
FspBI	CTAG	2 cut(s)	410, 789
GlaI	GCGC	3 cut(s)	67, 957, 1639
GluI	GCNGC	1 cut(s)	1678
GsaI	CCCAGC	2 cut(s)	498, 1259
GsuI	CTGGAG	1 cut(s)	1338
HaeII	RGCGCY	1 cut(s)	69
HaeIII	GGCC	5 cut(s)	430, 486, 1305, 1475, 1799
HapII	CCGG	8 cut(s)	20, 87, 98, 256, 319, 1000, 1117, 1854
HgaI	GACGC	2 cut(s)	79, 449
HhaI	GCGC	3 cut(s)	68, 958, 1640
Hin1I	GRCGYC	2 cut(s)	66, 90
Hin1II	CATG	8 cut(s)	65, 391, 623, 1162, 1252, 1486, 1638, 1822
Hin6I	GCGC	3 cut(s)	66, 956, 1638
HinP1I	GCGC	3 cut(s)	66, 956, 1638
HincII	GTYRAC	2 cut(s)	324, 847
HindII	GTYRAC	2 cut(s)	324, 847
HindIII	AAGCTT	1 cut(s)	1772
HinfI	GANTC	7 cut(s)	160, 229, 465, 560, 582, 652, 1368
HpaI	GTTAAC	1 cut(s)	324
HpaII	CCGG	8 cut(s)	20, 87, 98, 256, 319, 1000, 1117, 1854
HphI	GGTGA	5 cut(s)	263, 1084, 1639, 1843, 1921
Hpy166II	GTNNAC	6 cut(s)	139, 324, 385, 847, 985, 1887
Hpy188I	TCNGA	8 cut(s)	300, 1018, 1238, 1296, 1367, 1506, 1529, 1814
Hpy188III	TCNNGA	8 cut(s)	98, 262, 713, 789, 1285, 1317, 1411, 1888
Hpy8I	GTNNAC	6 cut(s)	139, 324, 385, 847, 985, 1887
Hpy99I	CGWCG	2 cut(s)	95, 1909
HpyAV	CCTTC	2 cut(s)	85, 623
HpyCH4III	ACNGT	6 cut(s)	221, 451, 544, 691, 914, 1264
HpyCH4IV	ACGT	1 cut(s)	471
HpyCH4V	TGCA	8 cut(s)	198, 306, 647, 1136, 1232, 1677, 1829, 1838
HpyF10VI	GCNNNNNNNGC	3 cut(s)	29, 1178, 1835
HpyF3I	CTNAG	5 cut(s)	162, 297, 899, 1372, 1641
HpySE526I	ACGT	1 cut(s)	471
Hsp92I	GRCGYC	2 cut(s)	66, 90
Hsp92II	CATG	8 cut(s)	65, 391, 623, 1162, 1252, 1486, 1638, 1822
HspAI	GCGC	3 cut(s)	66, 956, 1638
KasI	GGCGCC	1 cut(s)	65
Kpn2I	TCCGGA	1 cut(s)	97
KspAI	GTTAAC	1 cut(s)	324
KspI	CCGCGG	1 cut(s)	250
LmnI	GCTCC	8 cut(s)	100, 249, 292, 448, 498, 1009, 1319, 1529
Lsp1109I	GCAGC	1 cut(s)	1689
LweI	GCATC	5 cut(s)	14, 322, 634, 787, 1838
MaeI	CTAG	2 cut(s)	410, 789
MaeII	ACGT	1 cut(s)	471
MaeIII	GTNAC	7 cut(s)	191, 251, 668, 815, 1072, 1175, 1849
MfeI	CAATTG	1 cut(s)	840
MflI	RGATCY	3 cut(s)	14, 1461, 1515
MhlI	GDGCHC	3 cut(s)	246, 480, 1006
Mly113I	GGCGCC	1 cut(s)	66
MlyI	GAGTC	2 cut(s)	154, 646
MmeI	TCCRAC	1 cut(s)	1018
Mph1103I	ATGCAT	1 cut(s)	1840
MroI	TCCGGA	1 cut(s)	97
MroXI	GAANNNNTTC	2 cut(s)	515, 1580
MseI	TTAA	9 cut(s)	132, 282, 323, 354, 600, 615, 659, 696, 1206
MslI	CAYNNNNRTG	2 cut(s)	624, 990
MspA1I	CMGCKG	1 cut(s)	249
MspI	CCGG	8 cut(s)	20, 87, 98, 256, 319, 1000, 1117, 1854
MspR9I	CCNGG	8 cut(s)	29, 87, 764, 947, 1001, 1118, 1349, 1477
MunI	CAATTG	1 cut(s)	840
Mva1269I	GAATGC	2 cut(s)	1232, 1838
MvaI	CCWGG	5 cut(s)	29, 764, 947, 1349, 1477
MvnI	CGCG	1 cut(s)	249
MwoI	GCNNNNNNNGC	3 cut(s)	29, 1178, 1835
NarI	GGCGCC	1 cut(s)	66
NciI	CCSGG	3 cut(s)	87, 1001, 1118
NcoI	CCATGG	1 cut(s)	61
NlaIII	CATG	8 cut(s)	65, 391, 623, 1162, 1252, 1486, 1638, 1822
NlaIV	GGNNCC	7 cut(s)	67, 294, 1005, 1304, 1431, 1517, 1858
NmeAIII	GCCGAG	1 cut(s)	1775
NmuCI	GTSAC	5 cut(s)	251, 668, 815, 1072, 1849
NsiI	ATGCAT	1 cut(s)	1840
NspI	RCATGY	2 cut(s)	1638, 1822
NspV	TTCGAA	1 cut(s)	680
PceI	AGGCCT	1 cut(s)	1475
PctI	GAATGC	2 cut(s)	1232, 1838
PdmI	GAANNNNTTC	2 cut(s)	515, 1580
PfeI	GAWTC	5 cut(s)	229, 465, 560, 582, 1368
PfoI	TCCNGGA	1 cut(s)	762
PkrI	GCNGC	1 cut(s)	1679
PleI	GAGTC	2 cut(s)	154, 646
PluTI	GGCGCC	1 cut(s)	69
PpsI	GAGTC	2 cut(s)	154, 646
PpuMI	RGGWCCY	1 cut(s)	1429
Psp5II	RGGWCCY	1 cut(s)	1429
Psp6I	CCWGG	5 cut(s)	27, 762, 945, 1347, 1475
PspEI	GGTNACC	1 cut(s)	251
PspFI	CCCAGC	2 cut(s)	494, 1255
PspGI	CCWGG	5 cut(s)	27, 762, 945, 1347, 1475
PspN4I	GGNNCC	7 cut(s)	67, 294, 1005, 1304, 1431, 1517, 1858
PspPI	GGNCC	3 cut(s)	1303, 1429, 1798
PspPPI	RGGWCCY	1 cut(s)	1429
PsrI	GAACNNNNNNTAC	2 cut(s)	1568, 1600
PstNI	CAGNNNCTG	1 cut(s)	1262
PsuI	RGATCY	3 cut(s)	14, 1461, 1515
RsaI	GTAC	6 cut(s)	223, 551, 1196, 1213, 1481, 1736
RsaNI	GTAC	6 cut(s)	222, 550, 1195, 1212, 1480, 1735
RseI	CAYNNNNRTG	2 cut(s)	624, 990
SacII	CCGCGG	1 cut(s)	250
SaqAI	TTAA	9 cut(s)	132, 282, 323, 354, 600, 615, 659, 696, 1206
SatI	GCNGC	1 cut(s)	1678
Sau96I	GGNCC	3 cut(s)	1303, 1429, 1798
SchI	GAGTC	2 cut(s)	154, 646
ScrFI	CCNGG	8 cut(s)	29, 87, 764, 947, 1001, 1118, 1349, 1477
SduI	GDGCHC	3 cut(s)	246, 480, 1006
SfaNI	GCATC	5 cut(s)	14, 322, 634, 787, 1838
SfcI	CTRYAG	1 cut(s)	447
SfoI	GGCGCC	1 cut(s)	67
Sfr303I	CCGCGG	1 cut(s)	250
SfuI	TTCGAA	1 cut(s)	680
SgrBI	CCGCGG	1 cut(s)	250
SinI	GGWCC	1 cut(s)	1429
SmiMI	CAYNNNNRTG	2 cut(s)	624, 990
SmlI	CTYRAG	3 cut(s)	711, 938, 1424
SmoI	CTYRAG	3 cut(s)	711, 938, 1424
SseBI	AGGCCT	1 cut(s)	1475
SsiI	CCGC	4 cut(s)	247, 249, 289, 1122
SspDI	GGCGCC	1 cut(s)	65
SspMI	CTAG	2 cut(s)	410, 789
StuI	AGGCCT	1 cut(s)	1475
StyD4I	CCNGG	8 cut(s)	27, 85, 762, 945, 999, 1116, 1347, 1475
StyI	CCWWGG	2 cut(s)	61, 1093
TaaI	ACNGT	6 cut(s)	221, 451, 544, 691, 914, 1264
TaiI	ACGT	1 cut(s)	474
TaqI	TCGA	5 cut(s)	261, 379, 680, 1542, 1917
TatI	WGTACW	2 cut(s)	221, 1194
TfiI	GAWTC	5 cut(s)	229, 465, 560, 582, 1368
Tru1I	TTAA	9 cut(s)	132, 282, 323, 354, 600, 615, 659, 696, 1206
Tru9I	TTAA	9 cut(s)	132, 282, 323, 354, 600, 615, 659, 696, 1206
TscAI	CASTG	3 cut(s)	616, 694, 1440
TseFI	GTSAC	5 cut(s)	251, 668, 815, 1072, 1849
TseI	GCWGC	1 cut(s)	1677
Tsp45I	GTSAC	5 cut(s)	251, 668, 815, 1072, 1849
TspGWI	ACGGA	1 cut(s)	179
TspRI	CASTG	3 cut(s)	616, 694, 1440
VpaK11BI	GGWCC	1 cut(s)	1429
XapI	RAATTY	2 cut(s)	758, 1164
XbaI	TCTAGA	1 cut(s)	788
XceI	RCATGY	2 cut(s)	1638, 1822
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1313, 1473
XmnI	GAANNNNTTC	2 cut(s)	515, 1580
XspI	CTAG	2 cut(s)	410, 789
Zsp2I	ATGCAT	1 cut(s)	1840

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.