Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0008207

Physical Location & Seq

Genomic Coordinates Reverse (-)

4405025 .. 4407558

Gene Structure 2534 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1056900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGCTGTTACAAAACTCAGATTTAAAGACTCAGCTTCCTTACTCATATTACTCTCTCCCTTACTGCCAATCTGAGAGAATCGTCGACAGTATAGAGAATCTTGGGGAAGTGCTCCGTGGTGAAAGGATCGAGAGCTCCCCCTATGTGAGCAAAGAAGAGGAATACTTTATCCACAACCATCTGGCATTTACTGTAAAGTACCATAAAGATCCGCGAACTGGACAGGTAAGAATTGTGGATTTGAGTGTTAAACATGAGTATGAAGGGAAATGGAATGATAGGACTCGTTTTACTAACTGTAACCCCCATTCGAAACATACTGTTGTTAATTCTAACTCTCCTCAAGAGGTTGCCGAGCAGCAAGAAATCGTATTTACCTATGATGTTGAATTCCAGGAGAGTGATGTGAAGTCGGCTTCTAGATGGGATGCTTATCTACGGATGAGTAACCAAATCCGTTGGTTTTCAATTGTCAACTCATGGATGGTTGTCTTCTTCCTTTCGGGTATGGTAGCGATGATTATGCTAAGAACACTCTACCGTGATATCTCCAAGTATAATGAACTTGAGACCCAGGAAGAAGCGCAAGAAGAACCAGGATGGAAACTTATCCACGGGGATGTCTTCAGGGCTCCAAATAATTCTGAATTGCTCTGTGTGTATGTTGGAACTGGTGTTCAATTGTTTGGAATGATACTGGTGACAATGATATTTGCTGTCCTAGGCTTCCTCTCCCCATCTAACCGGGGCGGTCTTATGACCACAATGCTCTTTCCTTTTGTCTTCATGGGACTTTTCGCCGGTTATGCCTCAGTTCGCTTGTACAAGACGTTTCAGGGTACAGAATGGATGAAAATCGCATTCAGGACTGCAGTCATGTTCCCAGCAATTATCTCTGCCATTGTCATTGTCTTGTACACTGTGATTTGGGGCCAGAACTCATCTGGAGCTGTGACAATTGGCACAATGTTGACCCTGGTCTTTTTTTGGTTCGGAATCTCAGTCCCACTCGTATTTGTGGGAGGCTATGTTGGGTTCAGGAAGCCAGCACTTGAGGATCCAGTAAAGACAAACAAAGTCCCAAGACAGATCCCAGAACAGGCCTGGTACATGAACCCAGTTTTCTCAATTCTAATCGGAGGGATCCTCCCATTCGGAGCAGTTTTCGTCGAACTATACTTCATCCTCCCTTCGATATGGCTGAACCAGTTCTACTACATGTTAAGTTTCCTCTTCTTGGTCTTCATCATCCTCATCATCACATGCGCCGAGATCACCATCGTCCTCTGCTACTTCCAGTTGTGCAGCGAGGATTACTTGTGGTGGTGGAGATCATACCTCACATCCGGCTCATCGGCTCTGTACGTCTTCCTCTACGCCACATTCTACTTCTTCACAAAGCTCGAAATCACAAAACTGGTCTCGGTCCTCTTATACTTCGGGTGCATGCTCATTGTTTCTTATGCATTCTTTGTTGTCACCGGAACAATCGGCTTCTACGCTTGCTTTTGGTTCACGAGGCTCATCTACTCATCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0006810 GO:0006873 GO:0006885 GO:0006897 GO:0006909 GO:0006950 GO:0007155 GO:0008150 GO:0009505 GO:0009628 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016192 GO:0019725 GO:0022607 GO:0022610 GO:0030003 GO:0030004 GO:0030312 GO:0030641 GO:0031090 GO:0031224 GO:0031410 GO:0031982 GO:0031984 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033157 GO:0034622 GO:0036293 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048518 GO:0048878 GO:0050789 GO:0050801 GO:0050896 GO:0051049 GO:0051050 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0060341 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0070201 GO:0070482 GO:0070861 GO:0070863 GO:0071840 GO:0071944 GO:0090087 GO:0090316 GO:0097708 GO:0098588 GO:0098657 GO:0098771 GO:0098791 GO:0098805 GO:1903827 GO:1903829 GO:1904951 GO:2000008 GO:2000010

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EMP70

Protein Analysis

513

Amino Acids

59.24

Weight (kDa)

6.04

Isoelectric Point (pI)

44.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EMP70	PF02990	51 - 472	1.7e-170	Endomembrane protein 70

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008207)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10840 AT5G25100 AT5G25100
fragaria_vesca	FvH4_2g41140
malus_domestica	MD08G1056300.v1.1 MD08G1056400.v1.1 MD08G1056900.v1.1 MD15G1040700.v1.1
prunus_persica	Prupe.1G393900_v2.0.a1
pyrus_communis	pycom08g04620 pycom08g04630 pycom15g03800
rosa_chinensis	RchiOBHm_Chr6g0299401
rosa_multiflora	Rmu_sc0000861.1_g000066 Rmu_sc0005509.1_g000001 Rmu_sc0013965.1_g000004 Rmu_sc0017526.1_g000004
rosa_roxburghii	Rroxscaffold_7G00168720
rosa_rugosa	Rorug06G0287800
rosa_samantha	Rh6BG407900 Rh6CG413700 Rh6DG400700
rosa_wichuraiana	Rw6G034980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	84
AccII	CGCG	1 cut(s)	214
AciI	CCGC	2 cut(s)	212, 750
AclWI	GGATC	7 cut(s)	134, 203, 1052, 1065, 1084, 1138, 1151
AcsI	RAATTY	1 cut(s)	389
AcuI	CTGAAG	1 cut(s)	610
AfaI	GTAC	6 cut(s)	200, 824, 841, 917, 1109, 1364
AfiI	CCNNNNNNNGG	3 cut(s)	218, 1099, 1237
AflIII	ACRYGT	1 cut(s)	1218
AgsI	TTSAA	3 cut(s)	389, 468, 680
AjnI	CCWGG	5 cut(s)	393, 573, 595, 975, 1103
AloI	GAACNNNNNNTCC	2 cut(s)	660, 692
AluBI	AGCT	4 cut(s)	34, 135, 950, 1402
AluI	AGCT	4 cut(s)	34, 135, 950, 1402
Alw21I	GWGCWC	2 cut(s)	114, 137
Alw26I	GTCTC	2 cut(s)	563, 1426
AlwI	GGATC	7 cut(s)	134, 203, 1052, 1065, 1084, 1138, 1151
AoxI	GGCC	2 cut(s)	931, 1101
ApeKI	GCWGC	2 cut(s)	358, 1305
ApoI	RAATTY	1 cut(s)	389
Asp700I	GAANNNNTTC	1 cut(s)	1208
AspA2I	CCTAGG	1 cut(s)	721
AspLEI	GCGC	2 cut(s)	586, 1268
AspS9I	GGNCC	2 cut(s)	931, 1426
AsuC2I	CCSGG	1 cut(s)	746
AsuHPI	GGTGA	4 cut(s)	131, 712, 1267, 1471
AsuII	TTCGAA	1 cut(s)	311
AvaII	GGWCC	1 cut(s)	1426
AvrII	CCTAGG	1 cut(s)	721
BamHI	GGATCC	2 cut(s)	1057, 1143
BanII	GRGCYC	2 cut(s)	137, 634
BauI	CACGAG	1 cut(s)	1516
BbsI	GAAGAC	5 cut(s)	484, 616, 775, 1234, 1360
Bbv12I	GWGCWC	2 cut(s)	114, 137
BbvI	GCAGC	2 cut(s)	370, 1317
BccI	CCATC	6 cut(s)	186, 417, 478, 594, 745, 1286
BcgI	CGANNNNNNTGC	2 cut(s)	505, 539
BciT130I	CCWGG	5 cut(s)	395, 575, 597, 977, 1105
BcnI	CCSGG	1 cut(s)	746
BcoDI	GTCTC	2 cut(s)	563, 1426
BfaI	CTAG	2 cut(s)	420, 722
BfmI	CTRYAG	1 cut(s)	870
BisI	GCNGC	2 cut(s)	359, 1306
BlnI	CCTAGG	1 cut(s)	721
BlsI	GCNGC	2 cut(s)	360, 1307
Bme1390I	CCNGG	6 cut(s)	395, 575, 597, 746, 977, 1105
Bme18I	GGWCC	1 cut(s)	1426
BmgT120I	GGNCC	2 cut(s)	931, 1426
BmiI	GGNNCC	4 cut(s)	633, 932, 1059, 1145
BmrFI	CCNGG	6 cut(s)	395, 575, 597, 746, 977, 1105
BmrI	ACTGGG	1 cut(s)	1112
BmsI	GCATC	1 cut(s)	418
BmuI	ACTGGG	1 cut(s)	1112
BoxI	GACNNNNGTC	2 cut(s)	872, 977
BpiI	GAAGAC	5 cut(s)	484, 616, 775, 1234, 1360
BplI	GAGNNNNNCTC	2 cut(s)	1131, 1163
BpmI	CTGGAG	1 cut(s)	966
Bpu14I	TTCGAA	1 cut(s)	311
BpuEI	CTTGAG	3 cut(s)	327, 587, 1073
BpuMI	CCSGG	1 cut(s)	746
BsaBI	GATNNNNATC	3 cut(s)	432, 854, 1277
BsaI	GGTCTC	2 cut(s)	563, 1426
BsaJI	CCNNGG	6 cut(s)	115, 573, 613, 721, 745, 975
BsaWI	WCCGGW	1 cut(s)	1481
BsaXI	ACNNNNNCTCC	2 cut(s)	1014, 1044
Bsc4I	CCNNNNNNNGG	3 cut(s)	218, 1099, 1237
Bse118I	RCCGGY	1 cut(s)	800
Bse1I	ACTGG	8 cut(s)	223, 676, 702, 1061, 1118, 1207, 1297, 1422
Bse8I	GATNNNNATC	3 cut(s)	432, 854, 1277
BseBI	CCWGG	5 cut(s)	395, 575, 597, 977, 1105
BseDI	CCNNGG	6 cut(s)	115, 573, 613, 721, 745, 975
BseGI	GGATG	9 cut(s)	433, 447, 489, 605, 625, 855, 1182, 1248, 1343
BseJI	GATNNNNATC	3 cut(s)	432, 854, 1277
BseLI	CCNNNNNNNGG	3 cut(s)	218, 1099, 1237
BseMII	CTCAG	5 cut(s)	30, 44, 63, 825, 1014
BseNI	ACTGG	8 cut(s)	223, 676, 702, 1061, 1118, 1207, 1297, 1422
BseRI	GAGGAG	1 cut(s)	330
BseXI	GCAGC	2 cut(s)	370, 1317
BseYI	CCCAGC	1 cut(s)	883
BsgI	GTGCAG	1 cut(s)	1324
Bsh1236I	CGCG	1 cut(s)	214
BshFI	GGCC	2 cut(s)	933, 1103
BsiHKAI	GWGCWC	2 cut(s)	114, 137
BsiSI	CCGG	4 cut(s)	745, 801, 1347, 1482
BslFI	GGGAC	3 cut(s)	804, 989, 1064
BslI	CCNNNNNNNGG	3 cut(s)	218, 1099, 1237
BsmAI	GTCTC	2 cut(s)	563, 1426
BsmFI	GGGAC	3 cut(s)	804, 989, 1064
BsmI	GAATGC	2 cut(s)	860, 1466
BsnI	GGCC	2 cut(s)	933, 1103
Bso31I	GGTCTC	2 cut(s)	563, 1426
Bsp119I	TTCGAA	1 cut(s)	311
Bsp1286I	GDGCHC	3 cut(s)	114, 137, 634
Bsp1407I	TGTACA	2 cut(s)	822, 915
Bsp143I	GATC	7 cut(s)	126, 208, 1057, 1089, 1143, 1272, 1331
BspACI	CCGC	2 cut(s)	212, 750
BspANI	GGCC	2 cut(s)	933, 1103
BspCNI	CTCAG	5 cut(s)	29, 43, 64, 824, 1013
BspFNI	CGCG	1 cut(s)	214
BspLI	GGNNCC	4 cut(s)	633, 932, 1059, 1145
BspMAI	CTGCAG	1 cut(s)	874
BspPI	GGATC	7 cut(s)	134, 203, 1052, 1065, 1084, 1138, 1151
BspT104I	TTCGAA	1 cut(s)	311
BspTNI	GGTCTC	2 cut(s)	563, 1426
BsrFI	RCCGGY	1 cut(s)	800
BsrGI	TGTACA	2 cut(s)	822, 915
BsrI	ACTGG	8 cut(s)	223, 676, 702, 1061, 1118, 1207, 1297, 1422
BssAI	RCCGGY	1 cut(s)	800
BssECI	CCNNGG	6 cut(s)	115, 573, 613, 721, 745, 975
BssMI	GATC	7 cut(s)	126, 208, 1057, 1089, 1143, 1272, 1331
BssSI	CACGAG	1 cut(s)	1516
BssT1I	CCWWGG	1 cut(s)	721
Bst2BI	CACGAG	1 cut(s)	1516
Bst2UI	CCWGG	5 cut(s)	395, 575, 597, 977, 1105
Bst4CI	ACNGT	6 cut(s)	89, 193, 299, 322, 542, 922
Bst6I	CTCTTC	2 cut(s)	150, 1238
BstAUI	TGTACA	2 cut(s)	822, 915
BstBI	TTCGAA	1 cut(s)	311
BstC8I	GCNNGC	3 cut(s)	1047, 1448, 1504
BstDEI	CTNAG	6 cut(s)	16, 30, 72, 527, 811, 1000
BstDSI	CCRYGG	2 cut(s)	115, 613
BstF5I	GGATG	9 cut(s)	433, 447, 489, 605, 625, 855, 1182, 1248, 1343
BstFNI	CGCG	1 cut(s)	214
BstHHI	GCGC	2 cut(s)	586, 1268
BstKTI	GATC	7 cut(s)	129, 211, 1060, 1092, 1146, 1275, 1334
BstMAI	GTCTC	2 cut(s)	563, 1426
BstMBI	GATC	7 cut(s)	126, 208, 1057, 1089, 1143, 1272, 1331
BstMWI	GCNNNNNNNGC	1 cut(s)	806
BstNI	CCWGG	5 cut(s)	395, 575, 597, 977, 1105
BstNSI	RCATGY	3 cut(s)	1222, 1266, 1450
BstPAI	GACNNNNGTC	2 cut(s)	872, 977
BstSCI	CCNGG	6 cut(s)	393, 573, 595, 744, 975, 1103
BstSFI	CTRYAG	1 cut(s)	870
BstUI	CGCG	1 cut(s)	214
BstV1I	GCAGC	2 cut(s)	370, 1317
BstV2I	GAAGAC	5 cut(s)	484, 616, 775, 1234, 1360
BstX2I	RGATCY	4 cut(s)	208, 1057, 1089, 1143
BstYI	RGATCY	4 cut(s)	208, 1057, 1089, 1143
BsuRI	GGCC	2 cut(s)	933, 1103
BtgI	CCRYGG	2 cut(s)	115, 613
BtgZI	GCGATG	1 cut(s)	530
BtsCI	GGATG	9 cut(s)	433, 447, 489, 605, 625, 855, 1182, 1248, 1343
BtsIMutI	CAGTG	1 cut(s)	918
Cac8I	GCNNGC	3 cut(s)	1047, 1448, 1504
CfoI	GCGC	2 cut(s)	586, 1268
Cfr10I	RCCGGY	1 cut(s)	800
Cfr13I	GGNCC	2 cut(s)	931, 1426
Csp6I	GTAC	6 cut(s)	199, 823, 840, 916, 1108, 1363
CviAII	CATG	8 cut(s)	254, 480, 787, 877, 1111, 1219, 1263, 1447
CviQI	GTAC	6 cut(s)	199, 823, 840, 916, 1108, 1363
DdeI	CTNAG	6 cut(s)	16, 30, 72, 527, 811, 1000
DpnI	GATC	7 cut(s)	128, 210, 1059, 1091, 1145, 1274, 1333
DpnII	GATC	7 cut(s)	126, 208, 1057, 1089, 1143, 1272, 1331
DraI	TTTAAA	1 cut(s)	24
Eam1104I	CTCTTC	2 cut(s)	150, 1238
EarI	CTCTTC	2 cut(s)	150, 1238
Ecl136II	GAGCTC	1 cut(s)	135
Eco130I	CCWWGG	1 cut(s)	721
Eco147I	AGGCCT	1 cut(s)	1103
Eco24I	GRGCYC	2 cut(s)	137, 634
Eco31I	GGTCTC	2 cut(s)	563, 1426
Eco32I	GATATC	1 cut(s)	547
Eco47I	GGWCC	1 cut(s)	1426
Eco53kI	GAGCTC	1 cut(s)	135
Eco57I	CTGAAG	1 cut(s)	610
EcoICRI	GAGCTC	1 cut(s)	135
EcoRI	GAATTC	1 cut(s)	389
EcoRII	CCWGG	5 cut(s)	393, 573, 595, 975, 1103
EcoRV	GATATC	1 cut(s)	547
EcoT14I	CCWWGG	1 cut(s)	721
EcoT22I	ATGCAT	1 cut(s)	1468
EcoT38I	GRGCYC	2 cut(s)	137, 634
ErhI	CCWWGG	1 cut(s)	721
FaeI	CATG	8 cut(s)	257, 483, 790, 880, 1114, 1222, 1266, 1450
FaqI	GGGAC	3 cut(s)	804, 989, 1064
FatI	CATG	8 cut(s)	253, 479, 786, 876, 1110, 1218, 1262, 1446
FblI	GTMKAC	1 cut(s)	84
Fnu4HI	GCNGC	2 cut(s)	359, 1306
FokI	GGATG	9 cut(s)	440, 454, 496, 612, 632, 862, 1169, 1235, 1330
FriOI	GRGCYC	2 cut(s)	137, 634
Fsp4HI	GCNGC	2 cut(s)	359, 1306
FspBI	CTAG	2 cut(s)	420, 722
GlaI	GCGC	2 cut(s)	585, 1267
GluI	GCNGC	2 cut(s)	359, 1306
GsaI	CCCAGC	1 cut(s)	887
GsuI	CTGGAG	1 cut(s)	966
HaeIII	GGCC	2 cut(s)	933, 1103
HapII	CCGG	4 cut(s)	745, 801, 1347, 1482
HhaI	GCGC	2 cut(s)	586, 1268
Hin1II	CATG	8 cut(s)	257, 483, 790, 880, 1114, 1222, 1266, 1450
Hin6I	GCGC	2 cut(s)	584, 1266
HinP1I	GCGC	2 cut(s)	584, 1266
HincII	GTYRAC	3 cut(s)	85, 475, 972
HindII	GTYRAC	3 cut(s)	85, 475, 972
HinfI	GANTC	5 cut(s)	28, 78, 97, 283, 996
HpaII	CCGG	4 cut(s)	745, 801, 1347, 1482
HphI	GGTGA	4 cut(s)	131, 712, 1267, 1471
Hpy166II	GTNNAC	5 cut(s)	85, 475, 918, 972, 1515
Hpy188I	TCNGA	6 cut(s)	19, 73, 646, 995, 1139, 1157
Hpy188III	TCNNGA	7 cut(s)	130, 344, 420, 865, 945, 1039, 1516
Hpy8I	GTNNAC	5 cut(s)	85, 475, 918, 972, 1515
Hpy99I	CGWCG	2 cut(s)	86, 1172
HpyAV	CCTTC	2 cut(s)	257, 1200
HpyCH4III	ACNGT	6 cut(s)	89, 193, 299, 322, 542, 922
HpyCH4IV	ACGT	2 cut(s)	830, 1365
HpyCH4V	TGCA	4 cut(s)	872, 1305, 1446, 1466
HpyF10VI	GCNNNNNNNGC	1 cut(s)	806
HpyF3I	CTNAG	6 cut(s)	16, 30, 72, 527, 811, 1000
HpySE526I	ACGT	2 cut(s)	830, 1365
Hsp92II	CATG	8 cut(s)	257, 483, 790, 880, 1114, 1222, 1266, 1450
HspAI	GCGC	2 cut(s)	584, 1266
Kzo9I	GATC	7 cut(s)	126, 208, 1057, 1089, 1143, 1272, 1331
LmnI	GCTCC	5 cut(s)	117, 140, 637, 947, 1157
Lsp1109I	GCAGC	2 cut(s)	370, 1317
LweI	GCATC	1 cut(s)	418
MaeI	CTAG	2 cut(s)	420, 722
MaeII	ACGT	2 cut(s)	830, 1365
MaeIII	GTNAC	6 cut(s)	6, 299, 446, 700, 952, 1477
MalI	GATC	7 cut(s)	128, 210, 1059, 1091, 1145, 1274, 1333
MboI	GATC	7 cut(s)	126, 208, 1057, 1089, 1143, 1272, 1331
MfeI	CAATTG	3 cut(s)	468, 680, 957
MflI	RGATCY	4 cut(s)	208, 1057, 1089, 1143
MhlI	GDGCHC	3 cut(s)	114, 137, 634
MlyI	GAGTC	2 cut(s)	22, 277
MmeI	TCCRAC	1 cut(s)	646
Mph1103I	ATGCAT	1 cut(s)	1468
MroXI	GAANNNNTTC	1 cut(s)	1208
MseI	TTAA	4 cut(s)	23, 249, 327, 1223
MslI	CAYNNNNRTG	2 cut(s)	258, 618
MspI	CCGG	4 cut(s)	745, 801, 1347, 1482
MspR9I	CCNGG	6 cut(s)	395, 575, 597, 746, 977, 1105
MunI	CAATTG	3 cut(s)	468, 680, 957
Mva1269I	GAATGC	2 cut(s)	860, 1466
MvaI	CCWGG	5 cut(s)	395, 575, 597, 977, 1105
MvnI	CGCG	1 cut(s)	214
MwoI	GCNNNNNNNGC	1 cut(s)	806
NciI	CCSGG	1 cut(s)	746
NdeII	GATC	7 cut(s)	126, 208, 1057, 1089, 1143, 1272, 1331
NlaIII	CATG	8 cut(s)	257, 483, 790, 880, 1114, 1222, 1266, 1450
NlaIV	GGNNCC	4 cut(s)	633, 932, 1059, 1145
NmeAIII	GCCGAG	2 cut(s)	379, 1294
NmuCI	GTSAC	3 cut(s)	700, 952, 1477
NsiI	ATGCAT	1 cut(s)	1468
NspI	RCATGY	3 cut(s)	1222, 1266, 1450
NspV	TTCGAA	1 cut(s)	311
PaeI	GCATGC	1 cut(s)	1450
PceI	AGGCCT	1 cut(s)	1103
PciI	ACATGT	1 cut(s)	1218
PctI	GAATGC	2 cut(s)	860, 1466
PdmI	GAANNNNTTC	1 cut(s)	1208
PfeI	GAWTC	3 cut(s)	78, 97, 996
PfoI	TCCNGGA	1 cut(s)	393
PkrI	GCNGC	2 cut(s)	360, 1307
PleI	GAGTC	2 cut(s)	22, 277
PpsI	GAGTC	2 cut(s)	22, 277
PscI	ACATGT	1 cut(s)	1218
PshAI	GACNNNNGTC	2 cut(s)	872, 977
Psp124BI	GAGCTC	1 cut(s)	137
Psp6I	CCWGG	5 cut(s)	393, 573, 595, 975, 1103
PspFI	CCCAGC	1 cut(s)	883
PspGI	CCWGG	5 cut(s)	393, 573, 595, 975, 1103
PspN4I	GGNNCC	4 cut(s)	633, 932, 1059, 1145
PspPI	GGNCC	2 cut(s)	931, 1426
PsrI	GAACNNNNNNTAC	2 cut(s)	1196, 1228
PstI	CTGCAG	1 cut(s)	874
PsuI	RGATCY	4 cut(s)	208, 1057, 1089, 1143
RsaI	GTAC	6 cut(s)	200, 824, 841, 917, 1109, 1364
RsaNI	GTAC	6 cut(s)	199, 823, 840, 916, 1108, 1363
RseI	CAYNNNNRTG	2 cut(s)	258, 618
SacI	GAGCTC	1 cut(s)	137
SalI	GTCGAC	1 cut(s)	83
SaqAI	TTAA	4 cut(s)	23, 249, 327, 1223
SatI	GCNGC	2 cut(s)	359, 1306
Sau3AI	GATC	7 cut(s)	126, 208, 1057, 1089, 1143, 1272, 1331
Sau96I	GGNCC	2 cut(s)	931, 1426
SchI	GAGTC	2 cut(s)	22, 277
ScrFI	CCNGG	6 cut(s)	395, 575, 597, 746, 977, 1105
SduI	GDGCHC	3 cut(s)	114, 137, 634
SfaNI	GCATC	1 cut(s)	418
SfcI	CTRYAG	1 cut(s)	870
SfuI	TTCGAA	1 cut(s)	311
SinI	GGWCC	1 cut(s)	1426
SmiMI	CAYNNNNRTG	2 cut(s)	258, 618
SmlI	CTYRAG	3 cut(s)	342, 566, 1052
SmoI	CTYRAG	3 cut(s)	342, 566, 1052
SphI	GCATGC	1 cut(s)	1450
SseBI	AGGCCT	1 cut(s)	1103
SsiI	CCGC	2 cut(s)	212, 750
SspMI	CTAG	2 cut(s)	420, 722
SstI	GAGCTC	1 cut(s)	137
StuI	AGGCCT	1 cut(s)	1103
StyD4I	CCNGG	6 cut(s)	393, 573, 595, 744, 975, 1103
StyI	CCWWGG	1 cut(s)	721
TaaI	ACNGT	6 cut(s)	89, 193, 299, 322, 542, 922
TaiI	ACGT	2 cut(s)	833, 1368
TaqI	TCGA	6 cut(s)	84, 129, 311, 1170, 1193, 1404
TaqII	GACCGA	1 cut(s)	1414
TatI	WGTACW	2 cut(s)	822, 915
TfiI	GAWTC	3 cut(s)	78, 97, 996
Tru1I	TTAA	4 cut(s)	23, 249, 327, 1223
Tru9I	TTAA	4 cut(s)	23, 249, 327, 1223
TscAI	CASTG	1 cut(s)	925
TseFI	GTSAC	3 cut(s)	700, 952, 1477
TseI	GCWGC	2 cut(s)	358, 1305
Tsp45I	GTSAC	3 cut(s)	700, 952, 1477
TspDTI	ATGAA	7 cut(s)	276, 576, 775, 866, 1127, 1171, 1234
TspGWI	ACGGA	3 cut(s)	104, 446, 454
TspRI	CASTG	1 cut(s)	925
VpaK11BI	GGWCC	1 cut(s)	1426
XapI	RAATTY	1 cut(s)	389
XbaI	TCTAGA	1 cut(s)	419
XceI	RCATGY	3 cut(s)	1222, 1266, 1450
XcmI	CCANNNNNNNNNTGG	2 cut(s)	941, 1101
XmaJI	CCTAGG	1 cut(s)	721
XmiI	GTMKAC	1 cut(s)	84
XmnI	GAANNNNTTC	1 cut(s)	1208
XspI	CTAG	2 cut(s)	420, 722
Zsp2I	ATGCAT	1 cut(s)	1468

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.