Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0012530

Physical Location & Seq

Genomic Coordinates Reverse (-)

10128303 .. 10132669

Gene Structure 4367 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1113800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1695 bp

ATGGCCTCCATAACATTTTCAGGCGCTACTGGAAACTTCTCTTTTCTGCCAACAGCTGCCGTTTCTGAGAACTCAAATAATATAAAAGGTGGGTTTAATCCCTTAAAAAAGAACTTGAATAATACCATAAAAAAGCACAATGGTCGGAGCTTGGTGTCCACAAACGTAGCAAGTCCACCATTGCCGGCAATAGCTCATCCACCCCCAAAAGAAGAAAGTGGCGATGGTTACGACGGGAGTCACGTAGCATGGACGAGTGTGCGGCAGGAGAGATGGGAAGGAGAGCTGCTTGTCCAAGGAGAAATACCATTGTGGCTGAAGGGTACGTACCTAAGAAATGGTCCAGGGCTGTGGCACGTTGGGGGCTACAACTTCCGCCACCTCTTTGACGGCTATGCCATGCTCGCCAAACTCCACTTCCACGACGGCCGCCTCACTGCTGGCCACCGCCAGATCGAATCCGAGGCCTACACAGCCGCCATGAAGAACCAAAAATTATGTTTCCGTGAATTCTCTGAGGTCCCTAAGCCAGCCAATTTCCTATCTTATATTGGAGAACTAGCCAACTTATTTTCCGGTGCATCGTTGACTGACAACGCCAACACCGGAGTGGTTAAGCTAGGTGATGGTCGGGTTGTGTGCCTCACAGAAACCCAGAAGGGATCAATAGTGATTGACCCGACAACGTTGGACACTTTGGGAAAATTTGAGTACAGTGACACATTGGGTGGTTTGATACACTCGGCACATCCTATTGTGACCGATACCGAGTTTTTGACTTTGTTGCCGGATTTAATCAACCCGGGGTATTTAGCGGTCAGGATGAAGCCGGGTACTAATGAGAGGAAGGTGATCGGGCGGGTTGATTGTAGCCGTGGACCGGCTCCTGGTTGGGTGCACTCTTTCCCTGTGACCGAGCACTACATCATTGTGCCCGAAATGCCACTGAGGTATTGTGCTGGGAATTTGCTCAAGGCCGAGCCCACTCCCTTGTACAAGTTTGAGTGGCACCCTGACTCTAAGGCGTTTATGCATGTCATGTGTAAAGCTAGTGGCAACGTTGTGGCGAGTGTGGAAGTTCCATTGTATATTACGTTCCATTTCATTAATGCTTATGAGGAGACAGATGAGGACGGTAGGGTTACTGCTGTCATTGCCGATTGTTGTGAACACAACGCTGACACCACAATTCTGGATAAGCTTAAGATTCAGAATCTTCGGTCGTATACCGGCAAAGATGTACTACCAGATGCTCAGGTTGGTAGGTTCAAGATACCACTGGATGGGAGTCCATATGGAAAATTGGAGGCAGCACTAGACCCAAATGAGCATGGAAGAGGGATGGACATGTGCAGCATAAACCCTGCTTATCTGGGCAAAAAATACAGATACGCTTATGCTTGCGGGGCTCAGCGCCCGTGCAACTTCCCTAACACCCTCACCAAGATTGATTTGGTAGAGAAGAAAGCTAAGAATTGGTACGATGAGGGAGCAGTGCCATCCGAGCCGTTCTTTGTTGCCCGTCCCGGGTCAACTGAAGAAGATGATGGTGTTGTGATCTCGATGATTAGTGAGAAAAATGGAGATGGATATGCATTGCTGTTAGATGGATCAACGTTTGAAGAGATTGCTAGGGCAAAGTTCCCCTATGGTCTTCCATATGGACTGCATGGATGCTGGGTTCCCTCCAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001763 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006810 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008300 GO:0008610 GO:0009056 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009914 GO:0009926 GO:0009965 GO:0009987 GO:0010016 GO:0010033 GO:0010223 GO:0010346 GO:0010817 GO:0016042 GO:0016106 GO:0016108 GO:0016110 GO:0016114 GO:0016115 GO:0016116 GO:0016118 GO:0016119 GO:0016121 GO:0016122 GO:0016124 GO:0016491 GO:0016701 GO:0016702 GO:0018130 GO:0032501 GO:0032502 GO:0042214 GO:0042221 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046247 GO:0046483 GO:0048366 GO:0048367 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051179 GO:0051213 GO:0051234 GO:0055114 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:0099402 GO:1901334 GO:1901336 GO:1901360 GO:1901362 GO:1901575 GO:1901576 GO:1901600 GO:1901601 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko00906 map00906

Pfam Domains

Protein Families

RPE65

Protein Analysis

565

Amino Acids

62.01

Weight (kDa)

6.15

Isoelectric Point (pI)

30.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPE65	PF03055	86 - 562	1.4e-100	Retinal pigment epithelial membrane protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012530)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32810 AT4G32810
fragaria_vesca	FvH4_2g35610
malus_domestica	MD08G1113800.v1.1 MD15G1093500.v1.1
prunus_persica	Prupe.1G448400_v2.0.a1
pyrus_communis	pycom08g09450
rosa_chinensis	RchiOBHm_Chr6g0306201
rosa_laevigata	RLG00000010814 RLG00000011166
rosa_multiflora	Rmu_sc0003594.1_g000004
rosa_roxburghii	Rroxscaffold_7G00161940
rosa_rugosa	Rorug06G0348000 Rorug06G0348000
rosa_samantha	Rh6AG460500 Rh6BG440900 Rh6CG474500 Rh6DG460800
rosa_wichuraiana	Rw6G040110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1008
AccB7I	CCANNNNNTGG	1 cut(s)	1283
AccI	GTMKAC	1 cut(s)	1226
AciI	CCGC	8 cut(s)	262, 376, 430, 448, 477, 815, 859, 1404
AclI	AACGTT	3 cut(s)	686, 1059, 1616
AclWI	GGATC	2 cut(s)	670, 1618
AcoI	YGGCCR	2 cut(s)	427, 442
AcsI	RAATTY	3 cut(s)	509, 704, 964
AcuI	CTGAAG	2 cut(s)	338, 1557
AfaI	GTAC	7 cut(s)	325, 329, 713, 835, 995, 1242, 1481
AfiI	CCNNNNNNNGG	4 cut(s)	880, 887, 1283, 1527
AflII	CTTAAG	1 cut(s)	1202
AflIII	ACRYGT	1 cut(s)	1347
AgsI	TTSAA	3 cut(s)	118, 1270, 1622
AjnI	CCWGG	2 cut(s)	343, 886
AjuI	GAANNNNNNNTTGG	2 cut(s)	401, 433
AleI	CACNNNNGTG	1 cut(s)	608
AloI	GAACNNNNNNTCC	1 cut(s)	1665
AluBI	AGCT	8 cut(s)	56, 150, 194, 286, 619, 1049, 1201, 1469
AluI	AGCT	8 cut(s)	56, 150, 194, 286, 619, 1049, 1201, 1469
Alw21I	GWGCWC	2 cut(s)	900, 921
Alw26I	GTCTC	1 cut(s)	1115
Alw44I	GTGCAC	1 cut(s)	896
AlwI	GGATC	2 cut(s)	670, 1618
Ama87I	CYCGRG	2 cut(s)	802, 1526
AoxI	GGCC	5 cut(s)	3, 427, 442, 465, 975
ApaLI	GTGCAC	1 cut(s)	896
ApeKI	GCWGC	4 cut(s)	56, 286, 1310, 1353
ApoI	RAATTY	3 cut(s)	509, 704, 964
AseI	ATTAAT	1 cut(s)	1107
AspLEI	GCGC	2 cut(s)	26, 1416
AspS9I	GGNCC	3 cut(s)	341, 520, 878
AsuC2I	CCSGG	5 cut(s)	803, 804, 831, 1527, 1528
AsuHPI	GGTGA	3 cut(s)	635, 862, 1432
AvaI	CYCGRG	2 cut(s)	802, 1526
AvaII	GGWCC	3 cut(s)	341, 520, 878
BaeGI	GKGCMC	2 cut(s)	900, 936
BalI	TGGCCA	1 cut(s)	444
BanI	GGYRCC	1 cut(s)	1008
BanII	GRGCYC	2 cut(s)	984, 1411
BarI	GAAGNNNNNNTAC	2 cut(s)	311, 343
BbsI	GAAGAC	1 cut(s)	1646
Bbv12I	GWGCWC	2 cut(s)	900, 921
BbvI	GCAGC	4 cut(s)	43, 273, 1322, 1365
BccI	CCATC	9 cut(s)	218, 267, 620, 1277, 1336, 1507, 1541, 1580, 1601
BceAI	ACGGC	5 cut(s)	44, 406, 442, 858, 1492
BcgI	CGANNNNNNTGC	2 cut(s)	125, 159
BciT130I	CCWGG	2 cut(s)	345, 888
BcnI	CCSGG	5 cut(s)	803, 804, 831, 1527, 1528
BcoDI	GTCTC	1 cut(s)	1115
BfaI	CTAG	5 cut(s)	560, 620, 1050, 1316, 1632
BfoI	RGCGCY	2 cut(s)	27, 1417
BfrI	CTTAAG	1 cut(s)	1202
BisI	GCNGC	7 cut(s)	57, 263, 287, 430, 477, 1311, 1354
BlpI	GCTNAGC	1 cut(s)	1410
BlsI	GCNGC	7 cut(s)	58, 264, 288, 431, 478, 1312, 1355
Bme1390I	CCNGG	7 cut(s)	345, 803, 804, 831, 888, 1527, 1528
Bme18I	GGWCC	3 cut(s)	341, 520, 878
BmeT110I	CYCGRG	2 cut(s)	802, 1526
BmgT120I	GGNCC	3 cut(s)	341, 520, 878
BmiI	GGNNCC	4 cut(s)	522, 885, 1010, 1683
BmrFI	CCNGG	7 cut(s)	345, 803, 804, 831, 888, 1527, 1528
BmsI	GCATC	3 cut(s)	590, 1240, 1664
BoxI	GACNNNNGTC	1 cut(s)	237
BpiI	GAAGAC	1 cut(s)	1646
Bpu10I	CCTNAGC	2 cut(s)	525, 1254
Bpu1102I	GCTNAGC	1 cut(s)	1410
BpuEI	CTTGAG	1 cut(s)	956
BpuMI	CCSGG	5 cut(s)	803, 804, 831, 1527, 1528
BsaAI	YACGTR	2 cut(s)	244, 327
BsaJI	CCNNGG	7 cut(s)	295, 344, 462, 802, 803, 874, 1526
BsaWI	WCCGGW	2 cut(s)	575, 605
Bsc4I	CCNNNNNNNGG	4 cut(s)	880, 887, 1283, 1527
Bse118I	RCCGGY	3 cut(s)	184, 880, 1229
Bse1I	ACTGG	2 cut(s)	34, 1284
Bse3DI	GCAATG	3 cut(s)	179, 1152, 1595
BseBI	CCWGG	2 cut(s)	345, 888
BseDI	CCNNGG	7 cut(s)	295, 344, 462, 802, 803, 874, 1526
BseGI	GGATG	7 cut(s)	196, 748, 828, 1288, 1347, 1499, 1679
BseLI	CCNNNNNNNGG	4 cut(s)	880, 887, 1283, 1527
BseMI	GCAATG	3 cut(s)	179, 1152, 1595
BseMII	CTCAG	5 cut(s)	57, 507, 938, 1268, 1424
BseNI	ACTGG	2 cut(s)	34, 1284
BseRI	GAGGAG	1 cut(s)	1133
BseSI	GKGCMC	2 cut(s)	900, 936
BseX3I	CGGCCG	1 cut(s)	427
BseXI	GCAGC	4 cut(s)	43, 273, 1322, 1365
BseYI	CCCAGC	2 cut(s)	959, 1677
BsgI	GTGCAG	1 cut(s)	1372
Bsh1285I	CGRYCG	2 cut(s)	430, 1223
BshFI	GGCC	5 cut(s)	5, 429, 444, 467, 977
BshNI	GGYRCC	1 cut(s)	1008
BsiEI	CGRYCG	2 cut(s)	430, 1223
BsiHKAI	GWGCWC	2 cut(s)	900, 921
BsiHKCI	CYCGRG	2 cut(s)	802, 1526
BsiSI	CCGG	9 cut(s)	185, 576, 606, 788, 803, 830, 881, 1230, 1527
BslFI	GGGAC	2 cut(s)	506, 1509
BslI	CCNNNNNNNGG	4 cut(s)	880, 887, 1283, 1527
BsmAI	GTCTC	1 cut(s)	1115
BsmFI	GGGAC	2 cut(s)	506, 1509
BsnI	GGCC	5 cut(s)	5, 429, 444, 467, 977
BsoBI	CYCGRG	2 cut(s)	802, 1526
Bsp1286I	GDGCHC	5 cut(s)	900, 921, 936, 984, 1411
Bsp1407I	TGTACA	1 cut(s)	993
Bsp143I	GATC	5 cut(s)	453, 662, 852, 1557, 1610
Bsp1720I	GCTNAGC	1 cut(s)	1410
BspACI	CCGC	8 cut(s)	262, 376, 430, 448, 477, 815, 859, 1404
BspANI	GGCC	5 cut(s)	5, 429, 444, 467, 977
BspCNI	CTCAG	5 cut(s)	58, 508, 939, 1267, 1423
BspLI	GGNNCC	4 cut(s)	522, 885, 1010, 1683
BspPI	GGATC	2 cut(s)	670, 1618
BspT107I	GGYRCC	1 cut(s)	1008
BspTI	CTTAAG	1 cut(s)	1202
BsrDI	GCAATG	3 cut(s)	179, 1152, 1595
BsrFI	RCCGGY	3 cut(s)	184, 880, 1229
BsrGI	TGTACA	1 cut(s)	993
BsrI	ACTGG	2 cut(s)	34, 1284
BssAI	RCCGGY	3 cut(s)	184, 880, 1229
BssECI	CCNNGG	7 cut(s)	295, 344, 462, 802, 803, 874, 1526
BssMI	GATC	5 cut(s)	453, 662, 852, 1557, 1610
BssNAI	GTATAC	1 cut(s)	1227
BssT1I	CCWWGG	1 cut(s)	295
Bst1107I	GTATAC	1 cut(s)	1227
Bst2UI	CCWGG	2 cut(s)	345, 888
Bst4CI	ACNGT	2 cut(s)	716, 1136
Bst6I	CTCTTC	2 cut(s)	1330, 1617
BstAFI	CTTAAG	1 cut(s)	1202
BstAUI	TGTACA	1 cut(s)	993
BstBAI	YACGTR	2 cut(s)	244, 327
BstC8I	GCNNGC	5 cut(s)	186, 405, 442, 531, 1402
BstDEI	CTNAG	9 cut(s)	66, 332, 516, 525, 947, 1020, 1254, 1410, 1470
BstDSI	CCRYGG	1 cut(s)	874
BstF5I	GGATG	7 cut(s)	196, 748, 828, 1288, 1347, 1499, 1679
BstH2I	RGCGCY	2 cut(s)	27, 1417
BstHHI	GCGC	2 cut(s)	26, 1416
BstKTI	GATC	5 cut(s)	456, 665, 855, 1560, 1613
BstMAI	GTCTC	1 cut(s)	1115
BstMBI	GATC	5 cut(s)	453, 662, 852, 1557, 1610
BstMCI	CGRYCG	2 cut(s)	430, 1223
BstMWI	GCNNNNNNNGC	6 cut(s)	404, 473, 940, 1154, 1406, 1504
BstNI	CCWGG	2 cut(s)	345, 888
BstNSI	RCATGY	2 cut(s)	1037, 1351
BstPAI	GACNNNNGTC	1 cut(s)	237
BstSCI	CCNGG	7 cut(s)	343, 801, 802, 829, 886, 1525, 1526
BstSLI	GKGCMC	2 cut(s)	900, 936
BstSNI	TACGTA	1 cut(s)	327
BstV1I	GCAGC	4 cut(s)	43, 273, 1322, 1365
BstV2I	GAAGAC	1 cut(s)	1646
BstXI	CCANNNNNNTGG	2 cut(s)	351, 1192
BstZ17I	GTATAC	1 cut(s)	1227
BstZI	CGGCCG	1 cut(s)	427
BsuRI	GGCC	5 cut(s)	5, 429, 444, 467, 977
BtgI	CCRYGG	1 cut(s)	874
BtgZI	GCGATG	1 cut(s)	237
BtsCI	GGATG	7 cut(s)	196, 748, 828, 1288, 1347, 1499, 1679
BtsI	GCAGTG	2 cut(s)	435, 1500
BtsIMutI	CAGTG	5 cut(s)	435, 721, 944, 1277, 1500
Cac8I	GCNNGC	5 cut(s)	186, 405, 442, 531, 1402
CfoI	GCGC	2 cut(s)	26, 1416
Cfr10I	RCCGGY	3 cut(s)	184, 880, 1229
Cfr13I	GGNCC	3 cut(s)	341, 520, 878
Cfr9I	CCCGGG	2 cut(s)	802, 1526
Csp6I	GTAC	7 cut(s)	324, 328, 712, 834, 994, 1241, 1480
CviAII	CATG	8 cut(s)	249, 400, 481, 1034, 1039, 1331, 1348, 1670
CviQI	GTAC	7 cut(s)	324, 328, 712, 834, 994, 1241, 1480
DdeI	CTNAG	9 cut(s)	66, 332, 516, 525, 947, 1020, 1254, 1410, 1470
DpnI	GATC	5 cut(s)	455, 664, 854, 1559, 1612
DpnII	GATC	5 cut(s)	453, 662, 852, 1557, 1610
EaeI	YGGCCR	2 cut(s)	427, 442
EagI	CGGCCG	1 cut(s)	427
Eam1104I	CTCTTC	2 cut(s)	1330, 1617
EarI	CTCTTC	2 cut(s)	1330, 1617
EciI	GGCGGA	1 cut(s)	365
EclXI	CGGCCG	1 cut(s)	427
Eco105I	TACGTA	1 cut(s)	327
Eco130I	CCWWGG	1 cut(s)	295
Eco147I	AGGCCT	1 cut(s)	467
Eco24I	GRGCYC	2 cut(s)	984, 1411
Eco47I	GGWCC	3 cut(s)	341, 520, 878
Eco52I	CGGCCG	1 cut(s)	427
Eco57I	CTGAAG	2 cut(s)	338, 1557
Eco88I	CYCGRG	2 cut(s)	802, 1526
EcoO109I	RGGNCCY	1 cut(s)	520
EcoRI	GAATTC	1 cut(s)	509
EcoRII	CCWGG	2 cut(s)	343, 886
EcoT14I	CCWWGG	1 cut(s)	295
EcoT22I	ATGCAT	2 cut(s)	1035, 1597
EcoT38I	GRGCYC	2 cut(s)	984, 1411
ErhI	CCWWGG	1 cut(s)	295
FaeI	CATG	8 cut(s)	252, 403, 484, 1037, 1042, 1334, 1351, 1673
FaqI	GGGAC	2 cut(s)	506, 1509
FatI	CATG	8 cut(s)	248, 399, 480, 1033, 1038, 1330, 1347, 1669
FauI	CCCGC	2 cut(s)	852, 1397
FauNDI	CATATG	2 cut(s)	1294, 1660
FblI	GTMKAC	1 cut(s)	1226
Fnu4HI	GCNGC	7 cut(s)	57, 263, 287, 430, 477, 1311, 1354
FokI	GGATG	7 cut(s)	183, 735, 835, 1295, 1354, 1486, 1686
FriOI	GRGCYC	2 cut(s)	984, 1411
Fsp4HI	GCNGC	7 cut(s)	57, 263, 287, 430, 477, 1311, 1354
FspBI	CTAG	5 cut(s)	560, 620, 1050, 1316, 1632
GlaI	GCGC	2 cut(s)	25, 1415
GluI	GCNGC	7 cut(s)	57, 263, 287, 430, 477, 1311, 1354
GsaI	CCCAGC	2 cut(s)	963, 1681
HaeII	RGCGCY	2 cut(s)	27, 1417
HaeIII	GGCC	5 cut(s)	5, 429, 444, 467, 977
HapII	CCGG	9 cut(s)	185, 576, 606, 788, 803, 830, 881, 1230, 1527
HhaI	GCGC	2 cut(s)	26, 1416
Hin1II	CATG	8 cut(s)	252, 403, 484, 1037, 1042, 1334, 1351, 1673
Hin6I	GCGC	2 cut(s)	24, 1414
HinP1I	GCGC	2 cut(s)	24, 1414
HincII	GTYRAC	2 cut(s)	588, 1533
HindII	GTYRAC	2 cut(s)	588, 1533
HindIII	AAGCTT	1 cut(s)	1199
HinfI	GANTC	6 cut(s)	238, 458, 1016, 1207, 1213, 1288
HpaII	CCGG	9 cut(s)	185, 576, 606, 788, 803, 830, 881, 1230, 1527
HphI	GGTGA	3 cut(s)	635, 862, 1432
Hpy166II	GTNNAC	8 cut(s)	159, 176, 588, 878, 898, 1169, 1227, 1533
Hpy188I	TCNGA	6 cut(s)	67, 147, 463, 517, 1212, 1504
Hpy188III	TCNNGA	4 cut(s)	820, 1193, 1270, 1561
Hpy8I	GTNNAC	8 cut(s)	159, 176, 588, 878, 898, 1169, 1227, 1533
Hpy99I	CGWCG	2 cut(s)	236, 428
HpyAV	CCTTC	4 cut(s)	272, 313, 652, 841
HpyCH4III	ACNGT	2 cut(s)	716, 1136
HpyCH4IV	ACGT	8 cut(s)	165, 243, 326, 357, 686, 1059, 1094, 1616
HpyCH4V	TGCA	7 cut(s)	581, 898, 1033, 1353, 1422, 1595, 1669
HpyF10VI	GCNNNNNNNGC	6 cut(s)	404, 473, 940, 1154, 1406, 1504
HpyF3I	CTNAG	9 cut(s)	66, 332, 516, 525, 947, 1020, 1254, 1410, 1470
HpySE526I	ACGT	8 cut(s)	165, 243, 326, 357, 686, 1059, 1094, 1616
Hsp92II	CATG	8 cut(s)	252, 403, 484, 1037, 1042, 1334, 1351, 1673
HspAI	GCGC	2 cut(s)	24, 1414
KroI	GCCGGC	1 cut(s)	184
KroNI	GCCGGC	1 cut(s)	186
Kzo9I	GATC	5 cut(s)	453, 662, 852, 1557, 1610
LmnI	GCTCC	3 cut(s)	147, 889, 1490
Lsp1109I	GCAGC	4 cut(s)	43, 273, 1322, 1365
LweI	GCATC	3 cut(s)	590, 1240, 1664
MaeI	CTAG	5 cut(s)	560, 620, 1050, 1316, 1632
MaeII	ACGT	8 cut(s)	165, 243, 326, 357, 686, 1059, 1094, 1616
MaeIII	GTNAC	6 cut(s)	227, 239, 716, 757, 910, 1141
MalI	GATC	5 cut(s)	455, 664, 854, 1559, 1612
MboI	GATC	5 cut(s)	453, 662, 852, 1557, 1610
MboII	GAAGA	9 cut(s)	224, 496, 1208, 1347, 1474, 1550, 1553, 1634, 1646
MhlI	GDGCHC	5 cut(s)	900, 921, 936, 984, 1411
MlsI	TGGCCA	1 cut(s)	444
MluCI	AATT	8 cut(s)	494, 509, 535, 704, 964, 1188, 1301, 1474
MluNI	TGGCCA	1 cut(s)	444
MlyI	GAGTC	3 cut(s)	247, 1010, 1297
MmeI	TCCRAC	2 cut(s)	125, 669
Mox20I	TGGCCA	1 cut(s)	444
Mph1103I	ATGCAT	2 cut(s)	1035, 1597
MroNI	GCCGGC	1 cut(s)	184
MscI	TGGCCA	1 cut(s)	444
MseI	TTAA	6 cut(s)	96, 104, 615, 794, 1107, 1203
MslI	CAYNNNNRTG	2 cut(s)	608, 929
Msp20I	TGGCCA	1 cut(s)	444
MspA1I	CMGCKG	1 cut(s)	56
MspCI	CTTAAG	1 cut(s)	1202
MspI	CCGG	9 cut(s)	185, 576, 606, 788, 803, 830, 881, 1230, 1527
MspR9I	CCNGG	7 cut(s)	345, 803, 804, 831, 888, 1527, 1528
MvaI	CCWGG	2 cut(s)	345, 888
MwoI	GCNNNNNNNGC	6 cut(s)	404, 473, 940, 1154, 1406, 1504
NaeI	GCCGGC	1 cut(s)	186
NciI	CCSGG	5 cut(s)	803, 804, 831, 1527, 1528
NdeI	CATATG	2 cut(s)	1294, 1660
NdeII	GATC	5 cut(s)	453, 662, 852, 1557, 1610
NgoMIV	GCCGGC	1 cut(s)	184
NlaIII	CATG	8 cut(s)	252, 403, 484, 1037, 1042, 1334, 1351, 1673
NlaIV	GGNNCC	4 cut(s)	522, 885, 1010, 1683
NmeAIII	GCCGAG	2 cut(s)	722, 1003
NmuCI	GTSAC	4 cut(s)	239, 716, 757, 910
NsiI	ATGCAT	2 cut(s)	1035, 1597
NspI	RCATGY	2 cut(s)	1037, 1351
OliI	CACNNNNGTG	1 cut(s)	608
PceI	AGGCCT	1 cut(s)	467
PciI	ACATGT	1 cut(s)	1347
PcsI	WCGNNNNNNNCGW	1 cut(s)	240
PdiI	GCCGGC	1 cut(s)	186
PfeI	GAWTC	3 cut(s)	458, 1207, 1213
PflMI	CCANNNNNTGG	1 cut(s)	1283
PkrI	GCNGC	7 cut(s)	58, 264, 288, 431, 478, 1312, 1355
PleI	GAGTC	3 cut(s)	246, 1010, 1296
PpsI	GAGTC	3 cut(s)	246, 1010, 1296
Ppu21I	YACGTR	2 cut(s)	244, 327
PpuMI	RGGWCCY	1 cut(s)	520
PscI	ACATGT	1 cut(s)	1347
PshAI	GACNNNNGTC	1 cut(s)	237
PshBI	ATTAAT	1 cut(s)	1107
Psp1406I	AACGTT	3 cut(s)	686, 1059, 1616
Psp5II	RGGWCCY	1 cut(s)	520
Psp6I	CCWGG	2 cut(s)	343, 886
PspFI	CCCAGC	2 cut(s)	959, 1677
PspGI	CCWGG	2 cut(s)	343, 886
PspN4I	GGNNCC	4 cut(s)	522, 885, 1010, 1683
PspPI	GGNCC	3 cut(s)	341, 520, 878
PspPPI	RGGWCCY	1 cut(s)	520
PsrI	GAACNNNNNNTAC	2 cut(s)	1079, 1111
PvuII	CAGCTG	1 cut(s)	56
RsaI	GTAC	7 cut(s)	325, 329, 713, 835, 995, 1242, 1481
RsaNI	GTAC	7 cut(s)	324, 328, 712, 834, 994, 1241, 1480
RseI	CAYNNNNRTG	2 cut(s)	608, 929
SaqAI	TTAA	6 cut(s)	96, 104, 615, 794, 1107, 1203
SatI	GCNGC	7 cut(s)	57, 263, 287, 430, 477, 1311, 1354
Sau3AI	GATC	5 cut(s)	453, 662, 852, 1557, 1610
Sau96I	GGNCC	3 cut(s)	341, 520, 878
SchI	GAGTC	3 cut(s)	247, 1010, 1297
ScrFI	CCNGG	7 cut(s)	345, 803, 804, 831, 888, 1527, 1528
SduI	GDGCHC	5 cut(s)	900, 921, 936, 984, 1411
SfaNI	GCATC	3 cut(s)	590, 1240, 1664
SinI	GGWCC	3 cut(s)	341, 520, 878
SmaI	CCCGGG	2 cut(s)	804, 1528
SmiMI	CAYNNNNRTG	2 cut(s)	608, 929
SmlI	CTYRAG	2 cut(s)	971, 1202
SmoI	CTYRAG	2 cut(s)	971, 1202
SnaBI	TACGTA	1 cut(s)	327
Sse9I	AATT	8 cut(s)	494, 509, 535, 704, 964, 1188, 1301, 1474
SseBI	AGGCCT	1 cut(s)	467
SsiI	CCGC	8 cut(s)	262, 376, 430, 448, 477, 815, 859, 1404
SspMI	CTAG	5 cut(s)	560, 620, 1050, 1316, 1632
StuI	AGGCCT	1 cut(s)	467
StyD4I	CCNGG	7 cut(s)	343, 801, 802, 829, 886, 1525, 1526
StyI	CCWWGG	1 cut(s)	295
TaaI	ACNGT	2 cut(s)	716, 1136
TaiI	ACGT	8 cut(s)	168, 246, 329, 360, 689, 1062, 1097, 1619
TaqI	TCGA	2 cut(s)	456, 1562
TaqII	GACCGA	3 cut(s)	776, 929, 1209
TasI	AATT	8 cut(s)	494, 509, 535, 704, 964, 1188, 1301, 1474
TatI	WGTACW	3 cut(s)	711, 993, 1240
TauI	GCSGC	3 cut(s)	265, 432, 479
TfiI	GAWTC	3 cut(s)	458, 1207, 1213
Tru1I	TTAA	6 cut(s)	96, 104, 615, 794, 1107, 1203
Tru9I	TTAA	6 cut(s)	96, 104, 615, 794, 1107, 1203
TscAI	CASTG	5 cut(s)	442, 721, 951, 1284, 1500
TseFI	GTSAC	4 cut(s)	239, 716, 757, 910
TseI	GCWGC	4 cut(s)	56, 286, 1310, 1353
Tsp45I	GTSAC	4 cut(s)	239, 716, 757, 910
TspDTI	ATGAA	3 cut(s)	497, 839, 1093
TspGWI	ACGGA	1 cut(s)	494
TspMI	CCCGGG	2 cut(s)	802, 1526
TspRI	CASTG	5 cut(s)	442, 721, 951, 1284, 1500
Van91I	CCANNNNNTGG	1 cut(s)	1283
Vha464I	CTTAAG	1 cut(s)	1202
VneI	GTGCAC	1 cut(s)	896
VpaK11BI	GGWCC	3 cut(s)	341, 520, 878
VspI	ATTAAT	1 cut(s)	1107
XapI	RAATTY	3 cut(s)	509, 704, 964
XceI	RCATGY	2 cut(s)	1037, 1351
XcmI	CCANNNNNNNNNTGG	2 cut(s)	607, 1450
XmaI	CCCGGG	2 cut(s)	802, 1526
XmiI	GTMKAC	1 cut(s)	1226
XspI	CTAG	5 cut(s)	560, 620, 1050, 1316, 1632
Zsp2I	ATGCAT	2 cut(s)	1035, 1597

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.