Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0012530

Physical Location & Seq

Genomic Coordinates Forward (+)

64988400 .. 64991315

Gene Structure 2916 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ27522

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1686 bp

ATGGCTTCCTTAGGATTTTCTGTTGGCAGTGGAAACTCGTGTTTGCTGCTGAGAGCAGCGGTTTCCGATCACTCCAATCCCAATAGTAGCAGAGATGGATTTAATCCCTTAAAGAAGGGCGCTAAAAGCAGGAAGCGCTTGACGTCGACAAACGTCGCAAGTCCTCCATTACCGGCTGTTGCTGCCCCTCATCTGCCGCCGAGTAAGGAAACTGATGATCGGAGAAGCCACGTAGCTTGGACCAGTGTCCGGCAGGAGAGATGGGAAGGAGAGCTGCATGTACAAGGACAAATACCACTGTGGCTAAAAGGAACGTACTTAAGAAATGGTCCGGGAGTTTGGCACATTGAGGACTACAACTTCCGTCACCTCTTCGACGGCTACGCAATGCTAGCCAGGCTTCACTTTGAAAACGGCCGTCTGATCGCTGGCCACCGCCAAGTTGAATCCGATGCTTACAAAGCTGCAATGAAGAGCAAAAAGTTATGCTACCGTGAGTTCTCAGAAGTTCCTAAACAGGATAATTTCCTATCCTACATTGGTGAGCTGGCCAACCTATTCTCTGGTGCATCTCTGACCGATAACGCCAACACGGGCGTGGTCCACCTCGGAGATGGCCGGGTCGTGTGCCTCACCGAGACCCAAAAAGGGTCCATAGTGGTTGACCCGGACACTTTGGACACAATAGGAAGATTCGAGTATACTGACACGTTGGGAGGTTTGATTCACTCGGCACATCCTATTGTGACAGAGTCCGAGTTCTTGACTTTGTTGCCTGATTTGATCAACCCGGGTTACTTGGCGGTGAGGATGGAGCCTGGTACGAATGAAAGGAGGGTGATCGGGAGGGTTGACTGCAGCCAAGGTCCGGCGCCAGGGTGGGTTCATAGCTTTCCGGTGACCGAGCACTACATAATTGCGCCTGAAATGCCGTTGAGGTATTGTGCAGGGAATTTGCTCAGGGCCGAACCGACACCATTGTACAAGTTTGAGTGGCATCCTGAGTCCAAGGCCTATATGCATGCCATGTGTAAAGCTAGTGGTAAAATTGTGGCGAGTGTGGAAGTGCCATTGTTTGTTACTTTCCATTTCATTAATGCCTATGAAGAGAAAGATGAGGATGGGAGAGTGACGGCGCTCATTGCAGATTGTTGCGAGCACAGCGCTGATGCCACAATCCTGGATCAGCTTAGGATACAGAATCTGCGTTCATATACTGGTGAAGAAGATGTGCTACCAGATGCTAGGGTTGGAAGGTTTAGAATACCATTCGATGGGAGTGCATATGGAGAACTAGAGGCAGCATTAGACCCAAATGAACATGGAAAAGGGATGGACATGTGCAGCATAAACCCGGCATTTTTGGGTAAGAAGTATAGATATGCCTATGCTTGTGGGGCTGTGCGCCCTTGCAACTTCCCCAACACGCTCACAAAGCTTGATTTGGTGGAGAAGAAAGCTAAGAATTGGTACGATGAGGGCGCAGTGCCATCTGAACCATTCTTTGTGGCCCGACCTGGCGCAACTGAAGAAGATGATGGTGTGGTGATCTCGATGATTAGTGAGAAAAATGGAGGCAGTTATGCTTTGATACTAGATGGAACCACGTTCGAAGAGATTGCTAGAGCCAAGTTCCCTTATGGCCTTCCATATGGGCTGCATGGATGCTGGGTTCCAAAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001763 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006810 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008300 GO:0008610 GO:0009056 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009914 GO:0009926 GO:0009965 GO:0009987 GO:0010016 GO:0010033 GO:0010223 GO:0010346 GO:0010817 GO:0016042 GO:0016106 GO:0016108 GO:0016110 GO:0016114 GO:0016115 GO:0016116 GO:0016118 GO:0016119 GO:0016121 GO:0016122 GO:0016124 GO:0016491 GO:0016701 GO:0016702 GO:0018130 GO:0032501 GO:0032502 GO:0042214 GO:0042221 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046247 GO:0046483 GO:0048366 GO:0048367 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051179 GO:0051213 GO:0051234 GO:0055114 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:0099402 GO:1901334 GO:1901336 GO:1901360 GO:1901362 GO:1901575 GO:1901576 GO:1901600 GO:1901601 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko00906 map00906

Pfam Domains

Protein Families

RPE65

Protein Analysis

561

Amino Acids

62.1

Weight (kDa)

6.04

Isoelectric Point (pI)

38.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPE65	PF03055	82 - 559	2.5e-100	Retinal pigment epithelial membrane protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012530)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32810 AT4G32810
fragaria_vesca	FvH4_2g35610
malus_domestica	MD08G1113800.v1.1 MD15G1093500.v1.1
prunus_persica	Prupe.1G448400_v2.0.a1
pyrus_communis	pycom08g09450
rosa_chinensis	RchiOBHm_Chr6g0306201
rosa_laevigata	RLG00000010814 RLG00000011166
rosa_multiflora	Rmu_sc0003594.1_g000004
rosa_roxburghii	Rroxscaffold_7G00161940
rosa_rugosa	Rorug06G0348000 Rorug06G0348000
rosa_samantha	Rh6AG460500 Rh6BG440900 Rh6CG474500 Rh6DG460800
rosa_wichuraiana	Rw6G040110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	146
AccB1I	GGYRCC	1 cut(s)	871
AccB7I	CCANNNNNTGG	1 cut(s)	1274
AccI	GTMKAC	2 cut(s)	146, 701
AciI	CCGC	4 cut(s)	59, 197, 436, 803
AclWI	GGATC	1 cut(s)	1191
AcoI	YGGCCR	4 cut(s)	415, 430, 549, 616
AcsI	RAATTY	1 cut(s)	952
AcuI	CTGAAG	1 cut(s)	1548
AcyI	GRCGYC	2 cut(s)	143, 872
AfaI	GTAC	5 cut(s)	282, 317, 823, 983, 1472
AfeI	AGCGCT	2 cut(s)	137, 1165
AfiI	CCNNNNNNNGG	5 cut(s)	249, 648, 868, 875, 1274
AflII	CTTAAG	1 cut(s)	319
AflIII	ACRYGT	2 cut(s)	708, 1338
AgsI	TTSAA	2 cut(s)	410, 446
AjnI	CCWGG	5 cut(s)	395, 817, 874, 1179, 1516
AleI	CACNNNNGTG	1 cut(s)	596
AloI	GAACNNNNNNTCC	1 cut(s)	1656
AluBI	AGCT	9 cut(s)	236, 274, 464, 547, 891, 1037, 1189, 1438, 1460
AluI	AGCT	9 cut(s)	236, 274, 464, 547, 891, 1037, 1189, 1438, 1460
Alw21I	GWGCWC	2 cut(s)	909, 1161
Alw26I	GTCTC	1 cut(s)	632
AlwI	GGATC	1 cut(s)	1191
AlwNI	CAGNNNCTG	1 cut(s)	1204
Ama87I	CYCGRG	1 cut(s)	790
Aor51HI	AGCGCT	2 cut(s)	137, 1165
AoxI	GGCC	8 cut(s)	415, 430, 549, 616, 963, 1011, 1509, 1642
ApeKI	GCWGC	9 cut(s)	46, 56, 182, 274, 464, 858, 1301, 1344, 1657
ApoI	RAATTY	1 cut(s)	952
AseI	ATTAAT	1 cut(s)	1095
AspLEI	GCGC	9 cut(s)	122, 138, 874, 922, 1138, 1166, 1407, 1484, 1523
AspS9I	GGNCC	7 cut(s)	240, 329, 601, 651, 866, 963, 1510
AsuC2I	CCSGG	6 cut(s)	333, 620, 668, 791, 792, 1355
AsuHPI	GGTGA	8 cut(s)	359, 554, 625, 817, 850, 910, 1232, 1558
AsuII	TTCGAA	1 cut(s)	1611
AsuNHI	GCTAGC	1 cut(s)	391
AvaI	CYCGRG	1 cut(s)	790
AvaII	GGWCC	5 cut(s)	240, 329, 601, 651, 866
AxyI	CCTNAGG	1 cut(s)	10
BalI	TGGCCA	2 cut(s)	432, 551
BanI	GGYRCC	1 cut(s)	871
BarI	GAAGNNNNNNTAC	2 cut(s)	1218, 1250
BauI	CACGAG	1 cut(s)	37
Bbv12I	GWGCWC	2 cut(s)	909, 1161
BbvI	GCAGC	9 cut(s)	33, 68, 169, 261, 451, 870, 1313, 1356, 1644
BceAI	ACGGC	5 cut(s)	394, 402, 430, 916, 1149
BcgI	CGANNNNNNTGC	4 cut(s)	1262, 1296, 1601, 1635
BciT130I	CCWGG	5 cut(s)	397, 819, 876, 1181, 1518
BciVI	GTATCC	1 cut(s)	1188
BclI	TGATCA	1 cut(s)	783
BcnI	CCSGG	6 cut(s)	333, 620, 668, 791, 792, 1355
BcoDI	GTCTC	1 cut(s)	632
BfaI	CTAG	6 cut(s)	392, 1038, 1245, 1295, 1595, 1623
BfmI	CTRYAG	1 cut(s)	856
BfoI	RGCGCY	5 cut(s)	123, 139, 875, 1139, 1167
BfrI	CTTAAG	1 cut(s)	319
BfuI	GTATCC	1 cut(s)	1188
Bme18I	GGWCC	5 cut(s)	240, 329, 601, 651, 866
BmeT110I	CYCGRG	1 cut(s)	790
BmgT120I	GGNCC	7 cut(s)	240, 329, 601, 651, 866, 963, 1510
BmiI	GGNNCC	5 cut(s)	652, 816, 873, 1603, 1674
BmsI	GCATC	6 cut(s)	442, 578, 1006, 1159, 1231, 1655
BmtI	GCTAGC	1 cut(s)	395
BoxI	GACNNNNGTC	2 cut(s)	152, 245
Bpu10I	CCTNAGC	2 cut(s)	959, 1190
Bpu14I	TTCGAA	1 cut(s)	1611
BpuMI	CCSGG	6 cut(s)	333, 620, 668, 791, 792, 1355
BsaAI	YACGTR	1 cut(s)	232
BsaHI	GRCGYC	2 cut(s)	143, 872
BsaI	GGTCTC	1 cut(s)	632
BsaJI	CCNNGG	5 cut(s)	607, 790, 862, 875, 1008
BsaWI	WCCGGW	1 cut(s)	895
Bsc4I	CCNNNNNNNGG	5 cut(s)	249, 648, 868, 875, 1274
Bse118I	RCCGGY	1 cut(s)	172
Bse1I	ACTGG	2 cut(s)	243, 1222
Bse21I	CCTNAGG	1 cut(s)	10
Bse3DI	GCAATG	3 cut(s)	393, 474, 1140
BseBI	CCWGG	5 cut(s)	397, 819, 876, 1181, 1518
BseDI	CCNNGG	5 cut(s)	607, 790, 862, 875, 1008
BseGI	GGATG	6 cut(s)	736, 816, 997, 1126, 1338, 1670
BseLI	CCNNNNNNNGG	5 cut(s)	249, 648, 868, 875, 1274
BseMI	GCAATG	3 cut(s)	393, 474, 1140
BseMII	CTCAG	4 cut(s)	41, 516, 973, 993
BseNI	ACTGG	2 cut(s)	243, 1222
BseX3I	CGGCCG	1 cut(s)	415
BseXI	GCAGC	9 cut(s)	33, 68, 169, 261, 451, 870, 1313, 1356, 1644
BseYI	CCCAGC	1 cut(s)	1668
BsgI	GTGCAG	2 cut(s)	966, 1363
Bsh1285I	CGRYCG	1 cut(s)	418
BshFI	GGCC	8 cut(s)	417, 432, 551, 618, 965, 1013, 1511, 1644
BshNI	GGYRCC	1 cut(s)	871
BsiEI	CGRYCG	1 cut(s)	418
BsiHKAI	GWGCWC	2 cut(s)	909, 1161
BsiHKCI	CYCGRG	1 cut(s)	790
BsiSI	CCGG	9 cut(s)	173, 250, 332, 619, 668, 791, 869, 896, 1355
BslI	CCNNNNNNNGG	5 cut(s)	249, 648, 868, 875, 1274
BsmAI	GTCTC	1 cut(s)	632
BsnI	GGCC	8 cut(s)	417, 432, 551, 618, 965, 1013, 1511, 1644
Bso31I	GGTCTC	1 cut(s)	632
BsoBI	CYCGRG	1 cut(s)	790
Bsp119I	TTCGAA	1 cut(s)	1611
Bsp1286I	GDGCHC	2 cut(s)	909, 1161
Bsp1407I	TGTACA	2 cut(s)	280, 981
Bsp143I	GATC	7 cut(s)	67, 217, 423, 783, 840, 1183, 1548
BspACI	CCGC	4 cut(s)	59, 197, 436, 803
BspANI	GGCC	8 cut(s)	417, 432, 551, 618, 965, 1013, 1511, 1644
BspCNI	CTCAG	4 cut(s)	42, 515, 972, 994
BspLI	GGNNCC	5 cut(s)	652, 816, 873, 1603, 1674
BspMAI	CTGCAG	1 cut(s)	860
BspOI	GCTAGC	1 cut(s)	395
BspPI	GGATC	1 cut(s)	1191
BspQI	GCTCTTC	1 cut(s)	467
BspT104I	TTCGAA	1 cut(s)	1611
BspT107I	GGYRCC	1 cut(s)	871
BspTI	CTTAAG	1 cut(s)	319
BspTNI	GGTCTC	1 cut(s)	632
BsrDI	GCAATG	3 cut(s)	393, 474, 1140
BsrFI	RCCGGY	1 cut(s)	172
BsrGI	TGTACA	2 cut(s)	280, 981
BsrI	ACTGG	2 cut(s)	243, 1222
BssAI	RCCGGY	1 cut(s)	172
BssECI	CCNNGG	5 cut(s)	607, 790, 862, 875, 1008
BssMI	GATC	7 cut(s)	67, 217, 423, 783, 840, 1183, 1548
BssNAI	GTATAC	1 cut(s)	702
BssNI	GRCGYC	2 cut(s)	143, 872
BssSI	CACGAG	1 cut(s)	37
BssT1I	CCWWGG	2 cut(s)	862, 1008
Bst1107I	GTATAC	1 cut(s)	702
Bst2BI	CACGAG	1 cut(s)	37
Bst2UI	CCWGG	5 cut(s)	397, 819, 876, 1181, 1518
Bst4CI	ACNGT	2 cut(s)	300, 494
Bst6I	CTCTTC	4 cut(s)	377, 467, 1101, 1608
BstACI	GRCGYC	2 cut(s)	143, 872
BstAFI	CTTAAG	1 cut(s)	319
BstAUI	TGTACA	2 cut(s)	280, 981
BstBAI	YACGTR	1 cut(s)	232
BstBI	TTCGAA	1 cut(s)	1611
BstC8I	GCNNGC	5 cut(s)	393, 430, 549, 1023, 1157
BstDEI	CTNAG	7 cut(s)	10, 50, 502, 959, 1002, 1190, 1461
BstEII	GGTNACC	1 cut(s)	898
BstF5I	GGATG	6 cut(s)	736, 816, 997, 1126, 1338, 1670
BstH2I	RGCGCY	5 cut(s)	123, 139, 875, 1139, 1167
BstHHI	GCGC	9 cut(s)	122, 138, 874, 922, 1138, 1166, 1407, 1484, 1523
BstKTI	GATC	7 cut(s)	70, 220, 426, 786, 843, 1186, 1551
BstMAI	GTCTC	1 cut(s)	632
BstMBI	GATC	7 cut(s)	67, 217, 423, 783, 840, 1183, 1548
BstMCI	CGRYCG	1 cut(s)	418
BstNI	CCWGG	5 cut(s)	397, 819, 876, 1181, 1518
BstNSI	RCATGY	3 cut(s)	281, 1025, 1342
BstPAI	GACNNNNGTC	2 cut(s)	152, 245
BstPI	GGTNACC	1 cut(s)	898
BstSFI	CTRYAG	1 cut(s)	856
BstV1I	GCAGC	9 cut(s)	33, 68, 169, 261, 451, 870, 1313, 1356, 1644
BstXI	CCANNNNNNTGG	1 cut(s)	1180
BstZ17I	GTATAC	1 cut(s)	702
BstZI	CGGCCG	1 cut(s)	415
Bsu36I	CCTNAGG	1 cut(s)	10
BsuI	GTATCC	1 cut(s)	1188
BsuRI	GGCC	8 cut(s)	417, 432, 551, 618, 965, 1013, 1511, 1644
BtsCI	GGATG	6 cut(s)	736, 816, 997, 1126, 1338, 1670
BtsI	GCAGTG	2 cut(s)	34, 1491
BtsIMutI	CAGTG	4 cut(s)	34, 250, 296, 1491
Cac8I	GCNNGC	5 cut(s)	393, 430, 549, 1023, 1157
CaiI	CAGNNNCTG	1 cut(s)	1204
CfoI	GCGC	9 cut(s)	122, 138, 874, 922, 1138, 1166, 1407, 1484, 1523
Cfr10I	RCCGGY	1 cut(s)	172
Cfr13I	GGNCC	7 cut(s)	240, 329, 601, 651, 866, 963, 1510
Cfr9I	CCCGGG	1 cut(s)	790
Csp6I	GTAC	5 cut(s)	281, 316, 822, 982, 1471
CspCI	CAANNNNNGTGG	2 cut(s)	218, 253
CviAII	CATG	6 cut(s)	278, 1022, 1027, 1322, 1339, 1661
CviQI	GTAC	5 cut(s)	281, 316, 822, 982, 1471
DdeI	CTNAG	7 cut(s)	10, 50, 502, 959, 1002, 1190, 1461
DinI	GGCGCC	1 cut(s)	873
DpnI	GATC	7 cut(s)	69, 219, 425, 785, 842, 1185, 1550
DpnII	GATC	7 cut(s)	67, 217, 423, 783, 840, 1183, 1548
EaeI	YGGCCR	4 cut(s)	415, 430, 549, 616
EagI	CGGCCG	1 cut(s)	415
Eam1104I	CTCTTC	4 cut(s)	377, 467, 1101, 1608
EarI	CTCTTC	4 cut(s)	377, 467, 1101, 1608
EclXI	CGGCCG	1 cut(s)	415
Eco130I	CCWWGG	2 cut(s)	862, 1008
Eco147I	AGGCCT	1 cut(s)	1013
Eco31I	GGTCTC	1 cut(s)	632
Eco47I	GGWCC	5 cut(s)	240, 329, 601, 651, 866
Eco47III	AGCGCT	2 cut(s)	137, 1165
Eco52I	CGGCCG	1 cut(s)	415
Eco57I	CTGAAG	1 cut(s)	1548
Eco81I	CCTNAGG	1 cut(s)	10
Eco88I	CYCGRG	1 cut(s)	790
Eco91I	GGTNACC	1 cut(s)	898
EcoO65I	GGTNACC	1 cut(s)	898
EcoRII	CCWGG	5 cut(s)	395, 817, 874, 1179, 1516
EcoT14I	CCWWGG	2 cut(s)	862, 1008
EcoT22I	ATGCAT	1 cut(s)	1023
EgeI	GGCGCC	1 cut(s)	873
EheI	GGCGCC	1 cut(s)	873
ErhI	CCWWGG	2 cut(s)	862, 1008
FaeI	CATG	6 cut(s)	281, 1025, 1030, 1325, 1342, 1664
FatI	CATG	6 cut(s)	277, 1021, 1026, 1321, 1338, 1660
FauNDI	CATATG	2 cut(s)	1285, 1651
FbaI	TGATCA	1 cut(s)	783
FblI	GTMKAC	2 cut(s)	146, 701
FokI	GGATG	6 cut(s)	723, 823, 984, 1133, 1345, 1677
FspBI	CTAG	6 cut(s)	392, 1038, 1245, 1295, 1595, 1623
GlaI	GCGC	9 cut(s)	121, 137, 873, 921, 1137, 1165, 1406, 1483, 1522
GsaI	CCCAGC	1 cut(s)	1672
HaeII	RGCGCY	5 cut(s)	123, 139, 875, 1139, 1167
HaeIII	GGCC	8 cut(s)	417, 432, 551, 618, 965, 1013, 1511, 1644
HapII	CCGG	9 cut(s)	173, 250, 332, 619, 668, 791, 869, 896, 1355
HhaI	GCGC	9 cut(s)	122, 138, 874, 922, 1138, 1166, 1407, 1484, 1523
Hin1I	GRCGYC	2 cut(s)	143, 872
Hin1II	CATG	6 cut(s)	281, 1025, 1030, 1325, 1342, 1664
Hin6I	GCGC	9 cut(s)	120, 136, 872, 920, 1136, 1164, 1405, 1482, 1521
HinP1I	GCGC	9 cut(s)	120, 136, 872, 920, 1136, 1164, 1405, 1482, 1521
HincII	GTYRAC	3 cut(s)	147, 664, 853
HindII	GTYRAC	3 cut(s)	147, 664, 853
HindIII	AAGCTT	1 cut(s)	1436
HinfI	GANTC	6 cut(s)	446, 693, 724, 752, 1004, 1201
HpaII	CCGG	9 cut(s)	173, 250, 332, 619, 668, 791, 869, 896, 1355
HphI	GGTGA	8 cut(s)	359, 554, 625, 817, 850, 910, 1232, 1558
Hpy166II	GTNNAC	5 cut(s)	147, 604, 664, 702, 853
Hpy188I	TCNGA	9 cut(s)	67, 222, 423, 451, 505, 576, 611, 757, 1495
Hpy188III	TCNNGA	4 cut(s)	763, 844, 1001, 1552
Hpy8I	GTNNAC	5 cut(s)	147, 604, 664, 702, 853
Hpy99I	CGWCG	3 cut(s)	148, 158, 380
HpyAV	CCTTC	4 cut(s)	109, 260, 1248, 1655
HpyCH4III	ACNGT	2 cut(s)	300, 494
HpyCH4IV	ACGT	6 cut(s)	143, 153, 231, 314, 710, 1607
HpyF3I	CTNAG	7 cut(s)	10, 50, 502, 959, 1002, 1190, 1461
HpySE526I	ACGT	6 cut(s)	143, 153, 231, 314, 710, 1607
Hsp92I	GRCGYC	2 cut(s)	143, 872
Hsp92II	CATG	6 cut(s)	281, 1025, 1030, 1325, 1342, 1664
HspAI	GCGC	9 cut(s)	120, 136, 872, 920, 1136, 1164, 1405, 1482, 1521
KasI	GGCGCC	1 cut(s)	871
Ksp22I	TGATCA	1 cut(s)	783
Kzo9I	GATC	7 cut(s)	67, 217, 423, 783, 840, 1183, 1548
LguI	GCTCTTC	1 cut(s)	467
LmnI	GCTCC	1 cut(s)	814
Lsp1109I	GCAGC	9 cut(s)	33, 68, 169, 261, 451, 870, 1313, 1356, 1644
LweI	GCATC	6 cut(s)	442, 578, 1006, 1159, 1231, 1655
MaeI	CTAG	6 cut(s)	392, 1038, 1245, 1295, 1595, 1623
MaeII	ACGT	6 cut(s)	143, 153, 231, 314, 710, 1607
MaeIII	GTNAC	6 cut(s)	365, 745, 794, 898, 1078, 1129
MalI	GATC	7 cut(s)	69, 219, 425, 785, 842, 1185, 1550
MboI	GATC	7 cut(s)	67, 217, 423, 783, 840, 1183, 1548
MhlI	GDGCHC	2 cut(s)	909, 1161
MlsI	TGGCCA	2 cut(s)	432, 551
MluCI	AATT	5 cut(s)	523, 915, 952, 1047, 1465
MluNI	TGGCCA	2 cut(s)	432, 551
Mly113I	GGCGCC	1 cut(s)	872
MlyI	GAGTC	2 cut(s)	761, 1013
MmeI	TCCRAC	1 cut(s)	1231
Mox20I	TGGCCA	2 cut(s)	432, 551
Mph1103I	ATGCAT	1 cut(s)	1023
MscI	TGGCCA	2 cut(s)	432, 551
MseI	TTAA	4 cut(s)	102, 110, 320, 1095
MslI	CAYNNNNRTG	1 cut(s)	596
Msp20I	TGGCCA	2 cut(s)	432, 551
MspA1I	CMGCKG	1 cut(s)	59
MspCI	CTTAAG	1 cut(s)	319
MspI	CCGG	9 cut(s)	173, 250, 332, 619, 668, 791, 869, 896, 1355
MvaI	CCWGG	5 cut(s)	397, 819, 876, 1181, 1518
NarI	GGCGCC	1 cut(s)	872
NciI	CCSGG	6 cut(s)	333, 620, 668, 791, 792, 1355
NdeI	CATATG	2 cut(s)	1285, 1651
NdeII	GATC	7 cut(s)	67, 217, 423, 783, 840, 1183, 1548
NheI	GCTAGC	1 cut(s)	391
NlaIII	CATG	6 cut(s)	281, 1025, 1030, 1325, 1342, 1664
NlaIV	GGNNCC	5 cut(s)	652, 816, 873, 1603, 1674
NmeAIII	GCCGAG	2 cut(s)	225, 710
NmuCI	GTSAC	4 cut(s)	365, 745, 898, 1129
NsiI	ATGCAT	1 cut(s)	1023
NspI	RCATGY	3 cut(s)	281, 1025, 1342
NspV	TTCGAA	1 cut(s)	1611
OliI	CACNNNNGTG	1 cut(s)	596
PaeI	GCATGC	1 cut(s)	1025
PceI	AGGCCT	1 cut(s)	1013
PciI	ACATGT	1 cut(s)	1338
PciSI	GCTCTTC	1 cut(s)	467
PfeI	GAWTC	4 cut(s)	446, 693, 724, 1201
PflFI	GACNNNGTC	1 cut(s)	751
PflMI	CCANNNNNTGG	1 cut(s)	1274
PfoI	TCCNGGA	2 cut(s)	331, 1179
PleI	GAGTC	2 cut(s)	760, 1012
PluTI	GGCGCC	1 cut(s)	875
PpsI	GAGTC	2 cut(s)	760, 1012
Ppu21I	YACGTR	1 cut(s)	232
PscI	ACATGT	1 cut(s)	1338
PshAI	GACNNNNGTC	2 cut(s)	152, 245
PshBI	ATTAAT	1 cut(s)	1095
Psp6I	CCWGG	5 cut(s)	395, 817, 874, 1179, 1516
PspEI	GGTNACC	1 cut(s)	898
PspFI	CCCAGC	1 cut(s)	1668
PspGI	CCWGG	5 cut(s)	395, 817, 874, 1179, 1516
PspN4I	GGNNCC	5 cut(s)	652, 816, 873, 1603, 1674
PspPI	GGNCC	7 cut(s)	240, 329, 601, 651, 866, 963, 1510
PstI	CTGCAG	1 cut(s)	860
PstNI	CAGNNNCTG	1 cut(s)	1204
PsyI	GACNNNGTC	1 cut(s)	751
RsaI	GTAC	5 cut(s)	282, 317, 823, 983, 1472
RsaNI	GTAC	5 cut(s)	281, 316, 822, 982, 1471
RseI	CAYNNNNRTG	1 cut(s)	596
SalI	GTCGAC	1 cut(s)	145
SapI	GCTCTTC	1 cut(s)	467
SaqAI	TTAA	4 cut(s)	102, 110, 320, 1095
Sau3AI	GATC	7 cut(s)	67, 217, 423, 783, 840, 1183, 1548
Sau96I	GGNCC	7 cut(s)	240, 329, 601, 651, 866, 963, 1510
SchI	GAGTC	2 cut(s)	761, 1013
SduI	GDGCHC	2 cut(s)	909, 1161
SfaNI	GCATC	6 cut(s)	442, 578, 1006, 1159, 1231, 1655
SfcI	CTRYAG	1 cut(s)	856
SfoI	GGCGCC	1 cut(s)	873
SfuI	TTCGAA	1 cut(s)	1611
SinI	GGWCC	5 cut(s)	240, 329, 601, 651, 866
SmaI	CCCGGG	1 cut(s)	792
SmiMI	CAYNNNNRTG	1 cut(s)	596
SmlI	CTYRAG	1 cut(s)	319
SmoI	CTYRAG	1 cut(s)	319
SphI	GCATGC	1 cut(s)	1025
Sse9I	AATT	5 cut(s)	523, 915, 952, 1047, 1465
SseBI	AGGCCT	1 cut(s)	1013
SsiI	CCGC	4 cut(s)	59, 197, 436, 803
SspDI	GGCGCC	1 cut(s)	871
SspMI	CTAG	6 cut(s)	392, 1038, 1245, 1295, 1595, 1623
StuI	AGGCCT	1 cut(s)	1013
StyI	CCWWGG	2 cut(s)	862, 1008
TaaI	ACNGT	2 cut(s)	300, 494
TaiI	ACGT	6 cut(s)	146, 156, 234, 317, 713, 1610
TaqI	TCGA	6 cut(s)	146, 375, 696, 1272, 1553, 1611
TaqII	GACCGA	2 cut(s)	593, 917
TasI	AATT	5 cut(s)	523, 915, 952, 1047, 1465
TatI	WGTACW	2 cut(s)	280, 981
TauI	GCSGC	1 cut(s)	199
TfiI	GAWTC	4 cut(s)	446, 693, 724, 1201
Tru1I	TTAA	4 cut(s)	102, 110, 320, 1095
Tru9I	TTAA	4 cut(s)	102, 110, 320, 1095
TscAI	CASTG	4 cut(s)	34, 250, 303, 1491
TseFI	GTSAC	4 cut(s)	365, 745, 898, 1129
TseI	GCWGC	9 cut(s)	46, 56, 182, 274, 464, 858, 1301, 1344, 1657
Tsp45I	GTSAC	4 cut(s)	365, 745, 898, 1129
TspDTI	ATGAA	7 cut(s)	485, 843, 875, 1081, 1119, 1200, 1332
TspGWI	ACGGA	1 cut(s)	353
TspMI	CCCGGG	1 cut(s)	790
TspRI	CASTG	4 cut(s)	34, 250, 303, 1491
Tth111I	GACNNNGTC	1 cut(s)	751
Van91I	CCANNNNNTGG	1 cut(s)	1274
Vha464I	CTTAAG	1 cut(s)	319
VpaK11BI	GGWCC	5 cut(s)	240, 329, 601, 651, 866
VspI	ATTAAT	1 cut(s)	1095
XapI	RAATTY	1 cut(s)	952
XceI	RCATGY	3 cut(s)	281, 1025, 1342
XcmI	CCANNNNNNNNNTGG	2 cut(s)	595, 611
XmaI	CCCGGG	1 cut(s)	790
XmiI	GTMKAC	2 cut(s)	146, 701
XspI	CTAG	6 cut(s)	392, 1038, 1245, 1295, 1595, 1623
ZraI	GACGTC	1 cut(s)	144
Zsp2I	ATGCAT	1 cut(s)	1023

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.