Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012530

Physical Location & Seq

Genomic Coordinates Reverse (-)

6469074 .. 6474295

Gene Structure 5222 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1093500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1695 bp

ATGGCTTCCATAACATTTTCAGGCACTACTGGAAACTTCTCTTTGCTGCCAACAGCTGCCGTTTCTGAGAACTCCAATCATATAAAAGATGGGTTTAATCCCTTAAAGAAGAACTTGAGTAATACTAGAAAAAAACACAATGGTCGGAGCTTGGTGTCCACAAACGTAGCAAGTCCACCATTGCCGGCAATAACTCATCCACCCCCAAAAAGAGAGAGCGGCAATGGTTACGACGGGAGTCACGTGGCATGGACGAGTGTGCGGCAGGAGAGATGGGAAGGAGAGTTGCTTGTCCAAGGAGAAATACCATTGTGGCTGAAAGGGACGTACCTAAGAAATGGTCCAGGGCTGTGGCACGTCGGGGACTACAACTTCCGCCACCTCTTTGACGGCTATGCCATGCTCGTCAAACTTCACTTCCACGACGGTCGCCTCACTGCTGGCCACCGCCAGATCGAATCCGAGGCCTACAAAGCCGCCATGAAGAACAACAAATTATGTTTCCGTGAATTCTCTGAGGTCCCTAAGCCTAACAATTTCCTATCTTATATTGGTGAGCTAGCCAACCTATTCTCCGGTGCATCACTGACTGACAACGCCAACACCGGAGTGGTTAAGCTCGCCGATGGTCGGGTTGTGTGCCTCACAGAGACCCAGAAAGGGTCAATAGTGATTGATCCGGCTACCTTGGAAACTGTAGGGAAATTTGAGTACAGTGACACATTGGGTGGCTTGATACACTCGGCACATCCTATCGTGACCGATACCGAGTTTTTGACTTTGTTGCCGGATTTAATCAACCCAGGATATTTGGCAGTCAGGATGGAGCCGGGTACTAATGAGAGGAAGGTGATCGGGCGGGTTGATTGTAGCCGTGGACCGGCTCCGGGTTGGGTGCACTCTTTCCCTGTGACCGAGCACTACATCATTGTGCCCGAGATGCCACTGAGGTATTGTGCTGGGAATTTGCTCAAGGCCGAACCCACTCCCTTGTACAAGTTTGAGTGGCACCCTGACTCCAAGGCGTTTATGCATGTTATGTGTAAAGCTAGTGGCAACATTGTGGCGAGTGTGGAAGTTCCATTATACATTACGTTCCATTTCATTAATGCTTATGAGGAGACAGATGAGGATGGTAGGGTGACTGCAGTCATTGCAGATTGTTGCGAACACAACGCTGACACAACGATTCTGGACAAGCTTAAGATACAAAATCTTCGGTCGTATACTGGCAAAGATGTACTACCAGATGCTAAGGTTGGTAGATTCAGAATACCACTGGATGGGAGTCCATATGGAAATTTGGAGGCAGCACTAGACCCAAATGAGCATGGAAGAGGGATGGACATGTGCAGCATAAACCCCGCTTTTCTGGGCCAAAAATACAGATACGCTTATGCTTGCGGAGCTCAGCGCCCATGCAACTTTCCTAACACCCTCACCAAGATTGATTTGGTAGAGAAGAAAGCTAAGAATTGGTACGATGAAGGGGCAGTGCCATCCGAACCGTTCTTTGTGGCCCGTCCTGGGGCGACCGAAGAAGATGATGGTGTTGTAATATCGATGATTAGTGCGAAAAATGGAGATGGATATGCGTTGTTATTAGATGGATCAACGTTCGAAGAGATTGCTAGGGCAAAGTTCCCCTATGGTCTTCCATATGGACTGCATGGATGCTGGGTTCCCTTCAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001763 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0006810 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008300 GO:0008610 GO:0009056 GO:0009058 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009914 GO:0009926 GO:0009965 GO:0009987 GO:0010016 GO:0010033 GO:0010223 GO:0010346 GO:0010817 GO:0016042 GO:0016106 GO:0016108 GO:0016110 GO:0016114 GO:0016115 GO:0016116 GO:0016118 GO:0016119 GO:0016121 GO:0016122 GO:0016124 GO:0016491 GO:0016701 GO:0016702 GO:0018130 GO:0032501 GO:0032502 GO:0042214 GO:0042221 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046247 GO:0046483 GO:0048366 GO:0048367 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051179 GO:0051213 GO:0051234 GO:0055114 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:0099402 GO:1901334 GO:1901336 GO:1901360 GO:1901362 GO:1901575 GO:1901576 GO:1901600 GO:1901601 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

ko00906 map00906

Pfam Domains

Protein Families

RPE65

Protein Analysis

565

Amino Acids

62.28

Weight (kDa)

6.27

Isoelectric Point (pI)

31.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPE65	PF03055	86 - 562	2e-100	Retinal pigment epithelial membrane protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012530)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32810 AT4G32810
fragaria_vesca	FvH4_2g35610
malus_domestica	MD08G1113800.v1.1 MD15G1093500.v1.1
prunus_persica	Prupe.1G448400_v2.0.a1
pyrus_communis	pycom08g09450
rosa_chinensis	RchiOBHm_Chr6g0306201
rosa_laevigata	RLG00000010814 RLG00000011166
rosa_multiflora	Rmu_sc0003594.1_g000004
rosa_roxburghii	Rroxscaffold_7G00161940
rosa_rugosa	Rorug06G0348000 Rorug06G0348000
rosa_samantha	Rh6AG460500 Rh6BG440900 Rh6CG474500 Rh6DG460800
rosa_wichuraiana	Rw6G040110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1008
AccB7I	CCANNNNNTGG	1 cut(s)	1283
AccBSI	CCGCTC	1 cut(s)	219
AccI	GTMKAC	1 cut(s)	1226
AciI	CCGC	8 cut(s)	219, 262, 376, 448, 477, 859, 1365, 1404
AclI	AACGTT	1 cut(s)	1616
AclWI	GGATC	2 cut(s)	671, 1618
AcoI	YGGCCR	1 cut(s)	442
AcsI	RAATTY	4 cut(s)	509, 704, 964, 1300
AcvI	CACGTG	1 cut(s)	244
AfaI	GTAC	6 cut(s)	329, 713, 835, 995, 1242, 1481
AfiI	CCNNNNNNNGG	7 cut(s)	630, 660, 880, 887, 1283, 1527, 1528
AflII	CTTAAG	1 cut(s)	1202
AflIII	ACRYGT	1 cut(s)	1347
AgsI	TTSAA	1 cut(s)	1690
AjiI	CACGTC	1 cut(s)	358
AjnI	CCWGG	3 cut(s)	343, 802, 1525
AleI	CACNNNNGTG	1 cut(s)	608
AloI	GAACNNNNNNTCC	1 cut(s)	1665
AluBI	AGCT	8 cut(s)	56, 150, 559, 619, 1049, 1201, 1409, 1469
AluI	AGCT	8 cut(s)	56, 150, 559, 619, 1049, 1201, 1409, 1469
Alw21I	GWGCWC	3 cut(s)	900, 921, 1411
Alw26I	GTCTC	2 cut(s)	644, 1115
Alw44I	GTGCAC	1 cut(s)	896
AlwI	GGATC	2 cut(s)	671, 1618
Ama87I	CYCGRG	1 cut(s)	935
AoxI	GGCC	5 cut(s)	442, 465, 975, 1375, 1518
ApaLI	GTGCAC	1 cut(s)	896
ApeKI	GCWGC	4 cut(s)	46, 56, 1310, 1353
ApoI	RAATTY	4 cut(s)	509, 704, 964, 1300
AseI	ATTAAT	1 cut(s)	1107
AspLEI	GCGC	1 cut(s)	1416
AspS9I	GGNCC	5 cut(s)	341, 520, 878, 1375, 1519
AsuC2I	CCSGG	2 cut(s)	831, 888
AsuHPI	GGTGA	4 cut(s)	566, 862, 1153, 1432
AsuII	TTCGAA	1 cut(s)	1620
AsuNHI	GCTAGC	1 cut(s)	559
AvaI	CYCGRG	1 cut(s)	935
AvaII	GGWCC	3 cut(s)	341, 520, 878
BaeGI	GKGCMC	2 cut(s)	900, 936
BalI	TGGCCA	1 cut(s)	444
BanI	GGYRCC	1 cut(s)	1008
BanII	GRGCYC	1 cut(s)	1411
BbrPI	CACGTG	1 cut(s)	244
BbsI	GAAGAC	1 cut(s)	1646
Bbv12I	GWGCWC	3 cut(s)	900, 921, 1411
BbvI	GCAGC	4 cut(s)	33, 43, 1322, 1365
BceAI	ACGGC	3 cut(s)	44, 406, 858
BcgI	CGANNNNNNTGC	2 cut(s)	1610, 1644
BciT130I	CCWGG	3 cut(s)	345, 804, 1527
BcnI	CCSGG	2 cut(s)	831, 888
BcoDI	GTCTC	2 cut(s)	644, 1115
BfaI	CTAG	5 cut(s)	126, 560, 1050, 1316, 1632
BfmI	CTRYAG	2 cut(s)	696, 1146
BfoI	RGCGCY	1 cut(s)	1417
BfrI	CTTAAG	1 cut(s)	1202
BisI	GCNGC	7 cut(s)	47, 57, 220, 263, 477, 1311, 1354
BlpI	GCTNAGC	1 cut(s)	1410
BlsI	GCNGC	7 cut(s)	48, 58, 221, 264, 478, 1312, 1355
Bme1390I	CCNGG	5 cut(s)	345, 804, 831, 888, 1527
Bme18I	GGWCC	3 cut(s)	341, 520, 878
BmeT110I	CYCGRG	1 cut(s)	935
BmgBI	CACGTC	1 cut(s)	358
BmgT120I	GGNCC	5 cut(s)	341, 520, 878, 1375, 1519
BmiI	GGNNCC	5 cut(s)	522, 828, 885, 1010, 1683
BmrFI	CCNGG	5 cut(s)	345, 804, 831, 888, 1527
BmsI	GCATC	4 cut(s)	590, 930, 1240, 1664
BmtI	GCTAGC	1 cut(s)	563
BoxI	GACNNNNGTC	2 cut(s)	237, 1148
BpiI	GAAGAC	1 cut(s)	1646
Bpu10I	CCTNAGC	2 cut(s)	525, 1254
Bpu1102I	GCTNAGC	1 cut(s)	1410
Bpu14I	TTCGAA	1 cut(s)	1620
BpuEI	CTTGAG	2 cut(s)	136, 956
BpuMI	CCSGG	2 cut(s)	831, 888
Bsa29I	ATCGAT	1 cut(s)	1562
BsaAI	YACGTR	1 cut(s)	244
BsaI	GGTCTC	1 cut(s)	644
BsaJI	CCNNGG	8 cut(s)	295, 344, 462, 687, 802, 874, 1020, 1526
BsaWI	WCCGGW	2 cut(s)	575, 605
BsaXI	ACNNNNNCTCC	4 cut(s)	557, 587, 1001, 1031
Bsc4I	CCNNNNNNNGG	7 cut(s)	630, 660, 880, 887, 1283, 1527, 1528
Bse118I	RCCGGY	2 cut(s)	184, 880
Bse1I	ACTGG	3 cut(s)	34, 1234, 1284
Bse3DI	GCAATG	3 cut(s)	179, 229, 1152
BseBI	CCWGG	3 cut(s)	345, 804, 1527
BseCI	ATCGAT	1 cut(s)	1562
BseDI	CCNNGG	8 cut(s)	295, 344, 462, 687, 802, 874, 1020, 1526
BseGI	GGATG	8 cut(s)	196, 748, 828, 1138, 1288, 1347, 1499, 1679
BseLI	CCNNNNNNNGG	7 cut(s)	630, 660, 880, 887, 1283, 1527, 1528
BseMI	GCAATG	3 cut(s)	179, 229, 1152
BseMII	CTCAG	4 cut(s)	57, 507, 938, 1424
BseNI	ACTGG	3 cut(s)	34, 1234, 1284
BseRI	GAGGAG	1 cut(s)	1133
BseSI	GKGCMC	2 cut(s)	900, 936
BseXI	GCAGC	4 cut(s)	33, 43, 1322, 1365
BseYI	CCCAGC	2 cut(s)	959, 1677
BsgI	GTGCAG	1 cut(s)	1372
Bsh1285I	CGRYCG	3 cut(s)	430, 1223, 1536
BshFI	GGCC	5 cut(s)	444, 467, 977, 1377, 1520
BshNI	GGYRCC	1 cut(s)	1008
BshVI	ATCGAT	1 cut(s)	1562
BsiEI	CGRYCG	3 cut(s)	430, 1223, 1536
BsiHKAI	GWGCWC	3 cut(s)	900, 921, 1411
BsiHKCI	CYCGRG	1 cut(s)	935
BsiSI	CCGG	8 cut(s)	185, 576, 606, 680, 788, 830, 881, 887
BslFI	GGGAC	3 cut(s)	337, 377, 506
BslI	CCNNNNNNNGG	7 cut(s)	630, 660, 880, 887, 1283, 1527, 1528
BsmAI	GTCTC	2 cut(s)	644, 1115
BsmFI	GGGAC	3 cut(s)	337, 377, 506
BsnI	GGCC	5 cut(s)	444, 467, 977, 1377, 1520
Bso31I	GGTCTC	1 cut(s)	644
BsoBI	CYCGRG	1 cut(s)	935
Bsp119I	TTCGAA	1 cut(s)	1620
Bsp1286I	GDGCHC	4 cut(s)	900, 921, 936, 1411
Bsp1407I	TGTACA	1 cut(s)	993
Bsp143I	GATC	4 cut(s)	453, 676, 852, 1610
Bsp1720I	GCTNAGC	1 cut(s)	1410
BspACI	CCGC	8 cut(s)	219, 262, 376, 448, 477, 859, 1365, 1404
BspANI	GGCC	5 cut(s)	444, 467, 977, 1377, 1520
BspCNI	CTCAG	4 cut(s)	58, 508, 939, 1423
BspDI	ATCGAT	1 cut(s)	1562
BspLI	GGNNCC	5 cut(s)	522, 828, 885, 1010, 1683
BspMAI	CTGCAG	1 cut(s)	1150
BspOI	GCTAGC	1 cut(s)	563
BspPI	GGATC	2 cut(s)	671, 1618
BspT104I	TTCGAA	1 cut(s)	1620
BspT107I	GGYRCC	1 cut(s)	1008
BspTI	CTTAAG	1 cut(s)	1202
BspTNI	GGTCTC	1 cut(s)	644
BsrBI	CCGCTC	1 cut(s)	219
BsrDI	GCAATG	3 cut(s)	179, 229, 1152
BsrFI	RCCGGY	2 cut(s)	184, 880
BsrGI	TGTACA	1 cut(s)	993
BsrI	ACTGG	3 cut(s)	34, 1234, 1284
BssAI	RCCGGY	2 cut(s)	184, 880
BssECI	CCNNGG	8 cut(s)	295, 344, 462, 687, 802, 874, 1020, 1526
BssMI	GATC	4 cut(s)	453, 676, 852, 1610
BssNAI	GTATAC	1 cut(s)	1227
BssT1I	CCWWGG	3 cut(s)	295, 687, 1020
Bst1107I	GTATAC	1 cut(s)	1227
Bst2UI	CCWGG	3 cut(s)	345, 804, 1527
Bst4CI	ACNGT	4 cut(s)	428, 697, 716, 1509
Bst6I	CTCTTC	2 cut(s)	1330, 1617
BstAFI	CTTAAG	1 cut(s)	1202
BstAPI	GCANNNNNTGC	1 cut(s)	1154
BstAUI	TGTACA	1 cut(s)	993
BstBAI	YACGTR	1 cut(s)	244
BstBI	TTCGAA	1 cut(s)	1620
BstC8I	GCNNGC	5 cut(s)	186, 442, 561, 621, 1402
BstDEI	CTNAG	8 cut(s)	66, 332, 516, 525, 947, 1254, 1410, 1470
BstDSI	CCRYGG	1 cut(s)	874
BstF5I	GGATG	8 cut(s)	196, 748, 828, 1138, 1288, 1347, 1499, 1679
BstH2I	RGCGCY	1 cut(s)	1417
BstHHI	GCGC	1 cut(s)	1416
BstKTI	GATC	4 cut(s)	456, 679, 855, 1613
BstMAI	GTCTC	2 cut(s)	644, 1115
BstMBI	GATC	4 cut(s)	453, 676, 852, 1610
BstMCI	CGRYCG	3 cut(s)	430, 1223, 1536
BstMWI	GCNNNNNNNGC	4 cut(s)	473, 940, 1154, 1406
BstNI	CCWGG	3 cut(s)	345, 804, 1527
BstNSI	RCATGY	2 cut(s)	1037, 1351
BstPAI	GACNNNNGTC	2 cut(s)	237, 1148
BstSCI	CCNGG	5 cut(s)	343, 802, 829, 886, 1525
BstSFI	CTRYAG	2 cut(s)	696, 1146
BstSLI	GKGCMC	2 cut(s)	900, 936
BstV1I	GCAGC	4 cut(s)	33, 43, 1322, 1365
BstV2I	GAAGAC	1 cut(s)	1646
BstXI	CCANNNNNNTGG	1 cut(s)	351
BstZ17I	GTATAC	1 cut(s)	1227
Bsu15I	ATCGAT	1 cut(s)	1562
BsuRI	GGCC	5 cut(s)	444, 467, 977, 1377, 1520
BsuTUI	ATCGAT	1 cut(s)	1562
BtgI	CCRYGG	1 cut(s)	874
BtrI	CACGTC	1 cut(s)	358
BtsCI	GGATG	8 cut(s)	196, 748, 828, 1138, 1288, 1347, 1499, 1679
BtsI	GCAGTG	2 cut(s)	435, 1500
BtsIMutI	CAGTG	6 cut(s)	435, 584, 721, 944, 1277, 1500
Cac8I	GCNNGC	5 cut(s)	186, 442, 561, 621, 1402
CfoI	GCGC	1 cut(s)	1416
Cfr10I	RCCGGY	2 cut(s)	184, 880
Cfr13I	GGNCC	5 cut(s)	341, 520, 878, 1375, 1519
ClaI	ATCGAT	1 cut(s)	1562
Csp6I	GTAC	6 cut(s)	328, 712, 834, 994, 1241, 1480
CviAII	CATG	8 cut(s)	249, 400, 481, 1034, 1331, 1348, 1419, 1670
CviQI	GTAC	6 cut(s)	328, 712, 834, 994, 1241, 1480
DdeI	CTNAG	8 cut(s)	66, 332, 516, 525, 947, 1254, 1410, 1470
DpnI	GATC	4 cut(s)	455, 678, 854, 1612
DpnII	GATC	4 cut(s)	453, 676, 852, 1610
EaeI	YGGCCR	1 cut(s)	442
Eam1104I	CTCTTC	2 cut(s)	1330, 1617
EarI	CTCTTC	2 cut(s)	1330, 1617
EciI	GGCGGA	1 cut(s)	365
Ecl136II	GAGCTC	1 cut(s)	1409
Eco130I	CCWWGG	3 cut(s)	295, 687, 1020
Eco147I	AGGCCT	1 cut(s)	467
Eco24I	GRGCYC	1 cut(s)	1411
Eco31I	GGTCTC	1 cut(s)	644
Eco47I	GGWCC	3 cut(s)	341, 520, 878
Eco53kI	GAGCTC	1 cut(s)	1409
Eco72I	CACGTG	1 cut(s)	244
Eco88I	CYCGRG	1 cut(s)	935
EcoICRI	GAGCTC	1 cut(s)	1409
EcoO109I	RGGNCCY	1 cut(s)	520
EcoRI	GAATTC	1 cut(s)	509
EcoRII	CCWGG	3 cut(s)	343, 802, 1525
EcoT14I	CCWWGG	3 cut(s)	295, 687, 1020
EcoT22I	ATGCAT	1 cut(s)	1035
EcoT38I	GRGCYC	1 cut(s)	1411
ErhI	CCWWGG	3 cut(s)	295, 687, 1020
FaeI	CATG	8 cut(s)	252, 403, 484, 1037, 1334, 1351, 1422, 1673
FalI	AAGNNNNNCTT	2 cut(s)	98, 130
FaqI	GGGAC	3 cut(s)	337, 377, 506
FatI	CATG	8 cut(s)	248, 399, 480, 1033, 1330, 1347, 1418, 1669
FauI	CCCGC	2 cut(s)	852, 1372
FauNDI	CATATG	2 cut(s)	1294, 1660
FblI	GTMKAC	1 cut(s)	1226
Fnu4HI	GCNGC	7 cut(s)	47, 57, 220, 263, 477, 1311, 1354
FokI	GGATG	8 cut(s)	183, 735, 835, 1145, 1295, 1354, 1486, 1686
FriOI	GRGCYC	1 cut(s)	1411
Fsp4HI	GCNGC	7 cut(s)	47, 57, 220, 263, 477, 1311, 1354
FspBI	CTAG	5 cut(s)	126, 560, 1050, 1316, 1632
GlaI	GCGC	1 cut(s)	1415
GluI	GCNGC	7 cut(s)	47, 57, 220, 263, 477, 1311, 1354
GsaI	CCCAGC	2 cut(s)	963, 1681
HaeII	RGCGCY	1 cut(s)	1417
HaeIII	GGCC	5 cut(s)	444, 467, 977, 1377, 1520
HapII	CCGG	8 cut(s)	185, 576, 606, 680, 788, 830, 881, 887
HhaI	GCGC	1 cut(s)	1416
Hin1II	CATG	8 cut(s)	252, 403, 484, 1037, 1334, 1351, 1422, 1673
Hin6I	GCGC	1 cut(s)	1414
HinP1I	GCGC	1 cut(s)	1414
HindIII	AAGCTT	1 cut(s)	1199
HinfI	GANTC	6 cut(s)	238, 458, 1016, 1189, 1266, 1288
HpaII	CCGG	8 cut(s)	185, 576, 606, 680, 788, 830, 881, 887
HphI	GGTGA	4 cut(s)	566, 862, 1153, 1432
Hpy166II	GTNNAC	5 cut(s)	159, 176, 878, 898, 1227
Hpy188I	TCNGA	6 cut(s)	67, 147, 463, 517, 1271, 1504
Hpy188III	TCNNGA	3 cut(s)	757, 820, 1193
Hpy8I	GTNNAC	5 cut(s)	159, 176, 878, 898, 1227
Hpy99I	CGWCG	3 cut(s)	236, 362, 428
HpyAV	CCTTC	3 cut(s)	272, 841, 1481
HpyCH4III	ACNGT	4 cut(s)	428, 697, 716, 1509
HpyCH4IV	ACGT	6 cut(s)	165, 243, 326, 357, 1094, 1616
HpyCH4V	TGCA	8 cut(s)	581, 898, 1033, 1148, 1157, 1353, 1422, 1669
HpyF10VI	GCNNNNNNNGC	4 cut(s)	473, 940, 1154, 1406
HpyF3I	CTNAG	8 cut(s)	66, 332, 516, 525, 947, 1254, 1410, 1470
HpySE526I	ACGT	6 cut(s)	165, 243, 326, 357, 1094, 1616
Hsp92II	CATG	8 cut(s)	252, 403, 484, 1037, 1334, 1351, 1422, 1673
HspAI	GCGC	1 cut(s)	1414
KroI	GCCGGC	1 cut(s)	184
KroNI	GCCGGC	1 cut(s)	186
Kzo9I	GATC	4 cut(s)	453, 676, 852, 1610
LmnI	GCTCC	4 cut(s)	147, 826, 889, 1406
Lsp1109I	GCAGC	4 cut(s)	33, 43, 1322, 1365
LweI	GCATC	4 cut(s)	590, 930, 1240, 1664
MaeI	CTAG	5 cut(s)	126, 560, 1050, 1316, 1632
MaeII	ACGT	6 cut(s)	165, 243, 326, 357, 1094, 1616
MaeIII	GTNAC	6 cut(s)	227, 239, 716, 757, 910, 1141
MalI	GATC	4 cut(s)	455, 678, 854, 1612
MbiI	CCGCTC	1 cut(s)	219
MboI	GATC	4 cut(s)	453, 676, 852, 1610
MboII	GAAGA	9 cut(s)	121, 496, 1208, 1347, 1474, 1550, 1553, 1634, 1646
MhlI	GDGCHC	4 cut(s)	900, 921, 936, 1411
MlsI	TGGCCA	1 cut(s)	444
MluCI	AATT	7 cut(s)	494, 509, 535, 704, 964, 1300, 1474
MluNI	TGGCCA	1 cut(s)	444
MlyI	GAGTC	3 cut(s)	247, 1010, 1297
MmeI	TCCRAC	1 cut(s)	125
Mox20I	TGGCCA	1 cut(s)	444
Mph1103I	ATGCAT	1 cut(s)	1035
MroNI	GCCGGC	1 cut(s)	184
MscI	TGGCCA	1 cut(s)	444
MseI	TTAA	6 cut(s)	96, 104, 615, 794, 1107, 1203
MslI	CAYNNNNRTG	2 cut(s)	608, 929
Msp20I	TGGCCA	1 cut(s)	444
MspA1I	CMGCKG	1 cut(s)	56
MspCI	CTTAAG	1 cut(s)	1202
MspI	CCGG	8 cut(s)	185, 576, 606, 680, 788, 830, 881, 887
MspR9I	CCNGG	5 cut(s)	345, 804, 831, 888, 1527
MvaI	CCWGG	3 cut(s)	345, 804, 1527
MwoI	GCNNNNNNNGC	4 cut(s)	473, 940, 1154, 1406
NaeI	GCCGGC	1 cut(s)	186
NciI	CCSGG	2 cut(s)	831, 888
NdeI	CATATG	2 cut(s)	1294, 1660
NdeII	GATC	4 cut(s)	453, 676, 852, 1610
NgoMIV	GCCGGC	1 cut(s)	184
NheI	GCTAGC	1 cut(s)	559
NlaIII	CATG	8 cut(s)	252, 403, 484, 1037, 1334, 1351, 1422, 1673
NlaIV	GGNNCC	5 cut(s)	522, 828, 885, 1010, 1683
NmeAIII	GCCGAG	1 cut(s)	722
NmuCI	GTSAC	5 cut(s)	239, 716, 757, 910, 1141
NsiI	ATGCAT	1 cut(s)	1035
NspI	RCATGY	2 cut(s)	1037, 1351
NspV	TTCGAA	1 cut(s)	1620
OliI	CACNNNNGTG	1 cut(s)	608
PceI	AGGCCT	1 cut(s)	467
PciI	ACATGT	1 cut(s)	1347
PcsI	WCGNNNNNNNCGW	1 cut(s)	240
PdiI	GCCGGC	1 cut(s)	186
PfeI	GAWTC	3 cut(s)	458, 1189, 1266
PflMI	CCANNNNNTGG	1 cut(s)	1283
PkrI	GCNGC	7 cut(s)	48, 58, 221, 264, 478, 1312, 1355
PleI	GAGTC	3 cut(s)	246, 1010, 1296
PmaCI	CACGTG	1 cut(s)	244
PmlI	CACGTG	1 cut(s)	244
PpsI	GAGTC	3 cut(s)	246, 1010, 1296
Ppu21I	YACGTR	1 cut(s)	244
PpuMI	RGGWCCY	1 cut(s)	520
PscI	ACATGT	1 cut(s)	1347
PshAI	GACNNNNGTC	2 cut(s)	237, 1148
PshBI	ATTAAT	1 cut(s)	1107
Psp124BI	GAGCTC	1 cut(s)	1411
Psp1406I	AACGTT	1 cut(s)	1616
Psp5II	RGGWCCY	1 cut(s)	520
Psp6I	CCWGG	3 cut(s)	343, 802, 1525
PspCI	CACGTG	1 cut(s)	244
PspFI	CCCAGC	2 cut(s)	959, 1677
PspGI	CCWGG	3 cut(s)	343, 802, 1525
PspN4I	GGNNCC	5 cut(s)	522, 828, 885, 1010, 1683
PspPI	GGNCC	5 cut(s)	341, 520, 878, 1375, 1519
PspPPI	RGGWCCY	1 cut(s)	520
PstI	CTGCAG	1 cut(s)	1150
PvuII	CAGCTG	1 cut(s)	56
RsaI	GTAC	6 cut(s)	329, 713, 835, 995, 1242, 1481
RsaNI	GTAC	6 cut(s)	328, 712, 834, 994, 1241, 1480
RseI	CAYNNNNRTG	2 cut(s)	608, 929
SacI	GAGCTC	1 cut(s)	1411
SaqAI	TTAA	6 cut(s)	96, 104, 615, 794, 1107, 1203
SatI	GCNGC	7 cut(s)	47, 57, 220, 263, 477, 1311, 1354
Sau3AI	GATC	4 cut(s)	453, 676, 852, 1610
Sau96I	GGNCC	5 cut(s)	341, 520, 878, 1375, 1519
SchI	GAGTC	3 cut(s)	247, 1010, 1297
ScrFI	CCNGG	5 cut(s)	345, 804, 831, 888, 1527
SduI	GDGCHC	4 cut(s)	900, 921, 936, 1411
SfaNI	GCATC	4 cut(s)	590, 930, 1240, 1664
SfcI	CTRYAG	2 cut(s)	696, 1146
SfuI	TTCGAA	1 cut(s)	1620
SinI	GGWCC	3 cut(s)	341, 520, 878
SmiMI	CAYNNNNRTG	2 cut(s)	608, 929
SmlI	CTYRAG	3 cut(s)	115, 971, 1202
SmoI	CTYRAG	3 cut(s)	115, 971, 1202
Sse9I	AATT	7 cut(s)	494, 509, 535, 704, 964, 1300, 1474
SseBI	AGGCCT	1 cut(s)	467
SsiI	CCGC	8 cut(s)	219, 262, 376, 448, 477, 859, 1365, 1404
SspMI	CTAG	5 cut(s)	126, 560, 1050, 1316, 1632
SstI	GAGCTC	1 cut(s)	1411
StuI	AGGCCT	1 cut(s)	467
StyD4I	CCNGG	5 cut(s)	343, 802, 829, 886, 1525
StyI	CCWWGG	3 cut(s)	295, 687, 1020
TaaI	ACNGT	4 cut(s)	428, 697, 716, 1509
TaiI	ACGT	6 cut(s)	168, 246, 329, 360, 1097, 1619
TaqI	TCGA	3 cut(s)	456, 1562, 1620
TaqII	GACCGA	4 cut(s)	776, 929, 1209, 1550
TasI	AATT	7 cut(s)	494, 509, 535, 704, 964, 1300, 1474
TatI	WGTACW	3 cut(s)	711, 993, 1240
TauI	GCSGC	3 cut(s)	222, 265, 479
TfiI	GAWTC	3 cut(s)	458, 1189, 1266
Tru1I	TTAA	6 cut(s)	96, 104, 615, 794, 1107, 1203
Tru9I	TTAA	6 cut(s)	96, 104, 615, 794, 1107, 1203
TscAI	CASTG	6 cut(s)	442, 591, 721, 951, 1284, 1500
TseFI	GTSAC	5 cut(s)	239, 716, 757, 910, 1141
TseI	GCWGC	4 cut(s)	46, 56, 1310, 1353
Tsp45I	GTSAC	5 cut(s)	239, 716, 757, 910, 1141
TspDTI	ATGAA	3 cut(s)	497, 1093, 1500
TspGWI	ACGGA	1 cut(s)	494
TspRI	CASTG	6 cut(s)	442, 591, 721, 951, 1284, 1500
Van91I	CCANNNNNTGG	1 cut(s)	1283
Vha464I	CTTAAG	1 cut(s)	1202
VneI	GTGCAC	1 cut(s)	896
VpaK11BI	GGWCC	3 cut(s)	341, 520, 878
VspI	ATTAAT	1 cut(s)	1107
XapI	RAATTY	4 cut(s)	509, 704, 964, 1300
XceI	RCATGY	2 cut(s)	1037, 1351
XcmI	CCANNNNNNNNNTGG	2 cut(s)	607, 1450
XmiI	GTMKAC	1 cut(s)	1226
XspI	CTAG	5 cut(s)	126, 560, 1050, 1316, 1632
Zsp2I	ATGCAT	1 cut(s)	1035

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.