MD08G1125500.v1.1
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0019553
Physical Location & Seq
Reverse (-)
11797232 .. 11798122
891 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1125500.v1.1.491

Sequence Viewer

Length: 891 bp
ATGGAGCCTCTGGTGGGTGGAAGGTTTCAACTTGTTAGAAAGATCGGTAGGGGATCGTGTGGAGAGGTCTACCGAGGTACTAATATTCAGACAAACGAGGAGGTTGCTATTAAGCTCGAAAGTGGCAAGACGAGGCATCCACAACTGCTGTATGAATCGAAGTTGTATACAGTAGTGCATGGAGGACCTGGAATTCCAAACGTGAGAGCGTTTGGCTCTGAAGGAGACTACAATTTTCTTGTCATGGAATTACTTGGACCTAGTCTTGAGGATTTATTCCAAGTTTGCAATAGGAAATTCTCTCTTAAGACGGTTCTCATGCTCGCAGATAAAATGCTAGATCGACTAGAGTTTGTTCATTCGAAATCAGTCCTGCACCGCGACATCAAGCCAGACAATTTCGTAATGGGAGTGGGTCGACGAGCGAATCAGGTTTACATCATTGACTTAGGTCTCGCTAAGAAGTATAGAGACCCTTCAACTCATAGGCATATTCCTTATAGAGAAAATAAAAGTCTGATAGGTAATGCAAGGTATGCAAGCGTAAACACTCATCTTGGCATTGAACAAAGCTGCCGGGACGATTTGGAATCACTGGGATATGTGCTTATGTACTTTCTAAGAGGAAGTCTTCCTTGGCAGGGATTGAAAGTGGGATCAAAAAAGCAGAAAGATGAGAGCATCGTAGAAAAGAAAATGTCAACTTCTATTGAGTCCTTATGCACTGGTTACCCTTCAGAGTTTTCGGTATACTTCCTTTACTGTAGGTCGTTGCGGTTTGATGCGAAACCCGATTATGCTTCTCTGAAGAGACTCTTTCGTGACCTTTTTGTTCGTGAAGGTTTTCAGTTCGACTACGATTTTGATTGGGCTCGCTTAAACGTCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04392 map04011 map04392
Pfam Domains
Protein Families
Pkinase

Protein Analysis

297

Amino Acids

34.03

Weight (kDa)

9.21

Isoelectric Point (pI)

32.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 9 - 228 9.4e-26 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 10 - 202 2.8e-13 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 119 - 206 4e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019553)

Species Orthologous Gene IDs
malus_domestica MD08G1125500.v1.1 MD15G1105400.v1.1
pyrus_communis pycom08g10460 pycom15g09560
rosa_laevigata RLG00000023050 RLG00000036380
rosa_roxburghii Rroxscaffold_7G00212790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 4 cut(s) 69, 167, 418, 750
AccII CGCG 1 cut(s) 381
AciI CCGC 2 cut(s) 379, 775
AclWI GGATC 2 cut(s) 61, 664
AcsI RAATTY 2 cut(s) 192, 296
AcuI CTGAAG 3 cut(s) 240, 720, 827
AfaI GTAC 2 cut(s) 79, 614
AfiI CCNNNNNNNGG 2 cut(s) 14, 641
AflII CTTAAG 1 cut(s) 305
AgsI TTSAA 4 cut(s) 29, 480, 566, 649
AjnI CCWGG 1 cut(s) 187
AjuI GAANNNNNNNTTGG 2 cut(s) 619, 651
AluBI AGCT 2 cut(s) 115, 573
AluI AGCT 2 cut(s) 115, 573
Alw26I GTCTC 4 cut(s) 219, 458, 465, 805
AlwI GGATC 2 cut(s) 61, 664
ApeKI GCWGC 1 cut(s) 573
ApoI RAATTY 2 cut(s) 192, 296
ArsI GACNNNNNNTTYG 2 cut(s) 845, 877
Asp700I GAANNNNTTC 1 cut(s) 843
AspS9I GGNCC 2 cut(s) 185, 257
AsuC2I CCSGG 1 cut(s) 578
AsuII TTCGAA 1 cut(s) 362
AvaII GGWCC 2 cut(s) 185, 257
BanII GRGCYC 1 cut(s) 874
BbsI GAAGAC 1 cut(s) 623
BbvI GCAGC 1 cut(s) 560
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 1 cut(s) 189
BcnI CCSGG 1 cut(s) 578
BcoDI GTCTC 4 cut(s) 219, 458, 465, 805
BfaI CTAG 3 cut(s) 261, 338, 347
BfmI CTRYAG 1 cut(s) 763
BfrI CTTAAG 1 cut(s) 305
BisI GCNGC 1 cut(s) 574
BlsI GCNGC 1 cut(s) 575
Bme1390I CCNGG 2 cut(s) 189, 578
Bme18I GGWCC 2 cut(s) 185, 257
BmgT120I GGNCC 2 cut(s) 185, 257
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 2 cut(s) 189, 578
BmrI ACTGGG 1 cut(s) 605
BmsI GCATC 3 cut(s) 145, 690, 772
BmuI ACTGGG 1 cut(s) 605
BoxI GACNNNNGTC 1 cut(s) 450
BpiI GAAGAC 1 cut(s) 623
Bpu14I TTCGAA 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 578
BsaI GGTCTC 2 cut(s) 458, 465
BsaJI CCNNGG 2 cut(s) 73, 635
BsaWI WCCGGW 1 cut(s) 885
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 641
Bse1I ACTGG 2 cut(s) 600, 730
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 2 cut(s) 73, 635
BseGI GGATG 1 cut(s) 136
BseLI CCNNNNNNNGG 2 cut(s) 14, 641
BseNI ACTGG 2 cut(s) 600, 730
BseRI GAGGAG 1 cut(s) 113
BseXI GCAGC 1 cut(s) 560
BsgI GTGCAG 1 cut(s) 359
Bsh1236I CGCG 1 cut(s) 381
BsiSI CCGG 2 cut(s) 577, 886
BslFI GGGAC 1 cut(s) 593
BslI CCNNNNNNNGG 2 cut(s) 14, 641
BsmAI GTCTC 4 cut(s) 219, 458, 465, 805
BsmFI GGGAC 1 cut(s) 593
Bso31I GGTCTC 2 cut(s) 458, 465
Bsp119I TTCGAA 1 cut(s) 362
Bsp1286I GDGCHC 1 cut(s) 874
Bsp143I GATC 4 cut(s) 42, 53, 340, 656
BspACI CCGC 2 cut(s) 379, 775
BspFNI CGCG 1 cut(s) 381
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 2 cut(s) 61, 664
BspT104I TTCGAA 1 cut(s) 362
BspTI CTTAAG 1 cut(s) 305
BspTNI GGTCTC 2 cut(s) 458, 465
BsrI ACTGG 2 cut(s) 600, 730
BssECI CCNNGG 2 cut(s) 73, 635
BssMI GATC 4 cut(s) 42, 53, 340, 656
BssNAI GTATAC 2 cut(s) 168, 751
BssT1I CCWWGG 1 cut(s) 635
Bst1107I GTATAC 2 cut(s) 168, 751
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 3 cut(s) 172, 313, 764
Bst6I CTCTTC 1 cut(s) 803
BstAFI CTTAAG 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 536
BstBI TTCGAA 1 cut(s) 362
BstC8I GCNNGC 3 cut(s) 324, 541, 874
BstDEI CTNAG 3 cut(s) 448, 459, 620
BstEII GGTNACC 1 cut(s) 728
BstF5I GGATG 1 cut(s) 136
BstFNI CGCG 1 cut(s) 381
BstKTI GATC 4 cut(s) 45, 56, 343, 659
BstMAI GTCTC 4 cut(s) 219, 458, 465, 805
BstMBI GATC 4 cut(s) 42, 53, 340, 656
BstMWI GCNNNNNNNGC 1 cut(s) 536
BstNI CCWGG 1 cut(s) 189
BstPAI GACNNNNGTC 1 cut(s) 450
BstPI GGTNACC 1 cut(s) 728
BstSCI CCNGG 2 cut(s) 187, 576
BstSFI CTRYAG 1 cut(s) 763
BstUI CGCG 1 cut(s) 381
BstV1I GCAGC 1 cut(s) 560
BstV2I GAAGAC 1 cut(s) 623
BstZ17I GTATAC 2 cut(s) 168, 751
BtsCI GGATG 1 cut(s) 136
BtsIMutI CAGTG 2 cut(s) 593, 723
Cac8I GCNNGC 3 cut(s) 324, 541, 874
Cfr13I GGNCC 2 cut(s) 185, 257
Csp6I GTAC 2 cut(s) 78, 613
CviAII CATG 3 cut(s) 179, 244, 319
CviJI RGCY 6 cut(s) 7, 115, 216, 391, 573, 872
CviKI_1 RGCY 6 cut(s) 7, 115, 216, 391, 573, 872
CviQI GTAC 2 cut(s) 78, 613
DdeI CTNAG 3 cut(s) 448, 459, 620
DpnI GATC 4 cut(s) 44, 55, 342, 658
DpnII GATC 4 cut(s) 42, 53, 340, 656
Eam1104I CTCTTC 1 cut(s) 803
EarI CTCTTC 1 cut(s) 803
Eco130I CCWWGG 1 cut(s) 635
Eco24I GRGCYC 1 cut(s) 874
Eco31I GGTCTC 2 cut(s) 458, 465
Eco47I GGWCC 2 cut(s) 185, 257
Eco57I CTGAAG 3 cut(s) 240, 720, 827
Eco91I GGTNACC 1 cut(s) 728
EcoO109I RGGNCCY 1 cut(s) 185
EcoO65I GGTNACC 1 cut(s) 728
EcoRI GAATTC 1 cut(s) 192
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 635
EcoT38I GRGCYC 1 cut(s) 874
ErhI CCWWGG 1 cut(s) 635
FaeI CATG 3 cut(s) 182, 247, 322
FalI AAGNNNNNCTT 4 cut(s) 619, 651, 800, 832
FaqI GGGAC 1 cut(s) 593
FatI CATG 3 cut(s) 178, 243, 318
FblI GTMKAC 4 cut(s) 69, 167, 418, 750
Fnu4HI GCNGC 1 cut(s) 574
FokI GGATG 1 cut(s) 123
FriOI GRGCYC 1 cut(s) 874
Fsp4HI GCNGC 1 cut(s) 574
FspBI CTAG 3 cut(s) 261, 338, 347
GluI GCNGC 1 cut(s) 574
HapII CCGG 2 cut(s) 577, 886
Hin1II CATG 3 cut(s) 182, 247, 322
HincII GTYRAC 2 cut(s) 419, 702
HindII GTYRAC 2 cut(s) 419, 702
HinfI GANTC 5 cut(s) 155, 427, 590, 713, 813
HpaII CCGG 2 cut(s) 577, 886
Hpy166II GTNNAC 7 cut(s) 70, 168, 419, 436, 547, 702, 751
Hpy188I TCNGA 5 cut(s) 90, 220, 519, 739, 807
Hpy188III TCNNGA 3 cut(s) 266, 821, 836
Hpy8I GTNNAC 7 cut(s) 70, 168, 419, 436, 547, 702, 751
Hpy99I CGWCG 1 cut(s) 423
HpyAV CCTTC 5 cut(s) 15, 215, 486, 744, 833
HpyCH4III ACNGT 3 cut(s) 172, 313, 764
HpyCH4IV ACGT 2 cut(s) 201, 882
HpyCH4V TGCA 6 cut(s) 178, 288, 376, 530, 539, 723
HpyF10VI GCNNNNNNNGC 1 cut(s) 536
HpyF3I CTNAG 3 cut(s) 448, 459, 620
HpySE526I ACGT 2 cut(s) 201, 882
Hsp92II CATG 3 cut(s) 182, 247, 322
Kzo9I GATC 4 cut(s) 42, 53, 340, 656
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 9 cut(s) 174, 201, 386, 405, 416, 581, 590, 626, 711
Lsp1109I GCAGC 1 cut(s) 560
LweI GCATC 3 cut(s) 145, 690, 772
MaeI CTAG 3 cut(s) 261, 338, 347
MaeII ACGT 2 cut(s) 201, 882
MaeIII GTNAC 2 cut(s) 728, 821
MalI GATC 4 cut(s) 44, 55, 342, 658
MboI GATC 4 cut(s) 42, 53, 340, 656
MboII GAAGA 2 cut(s) 623, 820
MhlI GDGCHC 1 cut(s) 874
MluCI AATT 5 cut(s) 192, 232, 248, 296, 397
MlyI GAGTC 2 cut(s) 722, 807
MnlI CCTC 9 cut(s) 18, 58, 68, 91, 94, 126, 176, 262, 617
MroXI GAANNNNTTC 1 cut(s) 843
MseI TTAA 3 cut(s) 111, 306, 878
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 2 cut(s) 577, 886
MspR9I CCNGG 2 cut(s) 189, 578
MvaI CCWGG 1 cut(s) 189
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 1 cut(s) 536
NciI CCSGG 1 cut(s) 578
NdeII GATC 4 cut(s) 42, 53, 340, 656
NlaIII CATG 3 cut(s) 182, 247, 322
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 821
NspV TTCGAA 1 cut(s) 362
PdmI GAANNNNTTC 1 cut(s) 843
PfeI GAWTC 3 cut(s) 155, 427, 590
PflFI GACNNNGTC 1 cut(s) 261
PkrI GCNGC 1 cut(s) 575
PleI GAGTC 2 cut(s) 721, 807
PpsI GAGTC 2 cut(s) 721, 807
PpuMI RGGWCCY 1 cut(s) 185
PshAI GACNNNNGTC 1 cut(s) 450
Psp5II RGGWCCY 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 187
PspEI GGTNACC 1 cut(s) 728
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 185, 257
PspPPI RGGWCCY 1 cut(s) 185
PsyI GACNNNGTC 1 cut(s) 261
RsaI GTAC 2 cut(s) 79, 614
RsaNI GTAC 2 cut(s) 78, 613
SalI GTCGAC 1 cut(s) 417
SaqAI TTAA 3 cut(s) 111, 306, 878
SatI GCNGC 1 cut(s) 574
Sau3AI GATC 4 cut(s) 42, 53, 340, 656
Sau96I GGNCC 2 cut(s) 185, 257
SchI GAGTC 2 cut(s) 722, 807
ScrFI CCNGG 2 cut(s) 189, 578
SduI GDGCHC 1 cut(s) 874
SfaNI GCATC 3 cut(s) 145, 690, 772
SfcI CTRYAG 1 cut(s) 763
SfuI TTCGAA 1 cut(s) 362
SinI GGWCC 2 cut(s) 185, 257
SmlI CTYRAG 2 cut(s) 266, 305
SmoI CTYRAG 2 cut(s) 266, 305
Sse9I AATT 5 cut(s) 192, 232, 248, 296, 397
SsiI CCGC 2 cut(s) 379, 775
SspI AATATT 1 cut(s) 85
SspMI CTAG 3 cut(s) 261, 338, 347
StyD4I CCNGG 2 cut(s) 187, 576
StyI CCWWGG 1 cut(s) 635
TaaI ACNGT 3 cut(s) 172, 313, 764
TaiI ACGT 2 cut(s) 204, 885
TaqI TCGA 6 cut(s) 117, 158, 343, 362, 418, 852
TasI AATT 5 cut(s) 192, 232, 248, 296, 397
TatI WGTACW 1 cut(s) 612
TfiI GAWTC 3 cut(s) 155, 427, 590
Tru1I TTAA 3 cut(s) 111, 306, 878
Tru9I TTAA 3 cut(s) 111, 306, 878
TscAI CASTG 2 cut(s) 600, 730
TseFI GTSAC 1 cut(s) 821
TseI GCWGC 1 cut(s) 573
Tsp45I GTSAC 1 cut(s) 821
TspDTI ATGAA 2 cut(s) 168, 347
TspRI CASTG 2 cut(s) 600, 730
Tth111I GACNNNGTC 1 cut(s) 261
Vha464I CTTAAG 1 cut(s) 305
VpaK11BI GGWCC 2 cut(s) 185, 257
XapI RAATTY 2 cut(s) 192, 296
XmiI GTMKAC 4 cut(s) 69, 167, 418, 750
XmnI GAANNNNTTC 1 cut(s) 843
XspI CTAG 3 cut(s) 261, 338, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.