pycom15g09560
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0019553
Physical Location & Seq
Reverse (-)
6235031 .. 6235687
657 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g09560.1

Sequence Viewer

Length: 657 bp
ATGGATTTCCTTGGACCTAGTCTTGAGGATTTATTCCAAGCTTGCAGTAAGAAATTCTCTCTTAAGACGGTTCTCATGCTCGCAGATCAAATGATCGATCGAGTGGAGTTTGTTCATTCGAAATCAGTCCTGCACCGCGACATCAAGCCAGACAATTTCGTGATGGGAGCGGGTCGACGGGCGAATCAGGTTTACATCATCGACTTCGGTCTCGCTAAGAAGTATAGAAACTCTTTAACTCATCGGCATATTGCGTACAGAGACAATAAAAGTCTGGTAGGTACTGCAAGATATGCAAGCGTGAACAACCATCTTGGCATTGAACAAAGCCGCCGGGACGATTTGGAATCACTAGGATATGTGCTTTTGTACTTTCTAAGAGGAAGTCTTCCTTGGCAGGGTTTGAAAGCAGGGACAATAGAGCAGAAAGATGAGAATATCGCAGAAAAGAAAATATCAACTTCTATCGAGTCGCTATGCTCTGGTTACCCTTCCGAGTTTTCGGTATACTTCCATTACTGTAGGTCATTGACGTTTGATGCTAAACCAGATTATGCTTATCTGAAGAGACTCTTTAATAGCGTTTTTAAAAGGGAAGGTTTTCAGTTCGACTACGATTTTGATTGGACTCGATTGCTTAAACGTCTGGAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04392 map04011 map04392
Pfam Domains
Protein Families
Pkinase

Protein Analysis

219

Amino Acids

25.37

Weight (kDa)

8.99

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 7 - 79 6.6e-07 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 8 - 146 1.6e-17 Protein kinase domain
Pkinase_fungal PF17667 36 - 125 1.6e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019553)

Species Orthologous Gene IDs
malus_domestica MD08G1125500.v1.1 MD15G1105400.v1.1
pyrus_communis pycom08g10460 pycom15g09560
rosa_laevigata RLG00000023050 RLG00000036380
rosa_roxburghii Rroxscaffold_7G00212790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 170
AccI GTMKAC 2 cut(s) 175, 507
AccII CGCG 1 cut(s) 138
AciI CCGC 3 cut(s) 136, 170, 331
AcsI RAATTY 1 cut(s) 53
AcuI CTGAAG 1 cut(s) 584
AfaI GTAC 3 cut(s) 257, 283, 371
AfiI CCNNNNNNNGG 1 cut(s) 398
AflII CTTAAG 1 cut(s) 62
AgsI TTSAA 2 cut(s) 323, 406
AjuI GAANNNNNNNTTGG 2 cut(s) 376, 408
AluBI AGCT 1 cut(s) 41
AluI AGCT 1 cut(s) 41
Alw26I GTCTC 3 cut(s) 215, 255, 562
ApoI RAATTY 1 cut(s) 53
ArsI GACNNNNNNTTYG 2 cut(s) 602, 634
Asp700I GAANNNNTTC 1 cut(s) 600
AspS9I GGNCC 1 cut(s) 14
AsuC2I CCSGG 1 cut(s) 335
AsuII TTCGAA 1 cut(s) 119
AvaII GGWCC 1 cut(s) 14
BbsI GAAGAC 1 cut(s) 380
BccI CCATC 2 cut(s) 157, 318
BcnI CCSGG 1 cut(s) 335
BcoDI GTCTC 3 cut(s) 215, 255, 562
BfaI CTAG 2 cut(s) 18, 353
BfmI CTRYAG 1 cut(s) 520
BfrI CTTAAG 1 cut(s) 62
BisI GCNGC 1 cut(s) 331
BlsI GCNGC 1 cut(s) 332
Bme1390I CCNGG 1 cut(s) 335
Bme18I GGWCC 1 cut(s) 14
BmgT120I GGNCC 1 cut(s) 14
BmrFI CCNGG 1 cut(s) 335
BmsI GCATC 1 cut(s) 529
BoxI GACNNNNGTC 1 cut(s) 207
BpiI GAAGAC 1 cut(s) 380
Bpu14I TTCGAA 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 1 cut(s) 335
Bsa29I ATCGAT 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 2 cut(s) 10, 392
Bsc4I CCNNNNNNNGG 1 cut(s) 398
BseCI ATCGAT 1 cut(s) 96
BseDI CCNNGG 2 cut(s) 10, 392
BseLI CCNNNNNNNGG 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 116
Bsh1236I CGCG 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 100
BshVI ATCGAT 1 cut(s) 96
BsiEI CGRYCG 1 cut(s) 100
BsiSI CCGG 1 cut(s) 334
BslFI GGGAC 2 cut(s) 350, 427
BslI CCNNNNNNNGG 1 cut(s) 398
BsmAI GTCTC 3 cut(s) 215, 255, 562
BsmFI GGGAC 2 cut(s) 350, 427
Bso31I GGTCTC 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 119
Bsp143I GATC 3 cut(s) 85, 93, 97
BspACI CCGC 3 cut(s) 136, 170, 331
BspDI ATCGAT 1 cut(s) 96
BspFNI CGCG 1 cut(s) 138
BspT104I TTCGAA 1 cut(s) 119
BspTI CTTAAG 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 215
BsrBI CCGCTC 1 cut(s) 170
BssECI CCNNGG 2 cut(s) 10, 392
BssMI GATC 3 cut(s) 85, 93, 97
BssNAI GTATAC 1 cut(s) 508
BssT1I CCWWGG 2 cut(s) 10, 392
Bst1107I GTATAC 1 cut(s) 508
Bst4CI ACNGT 2 cut(s) 70, 521
Bst6I CTCTTC 1 cut(s) 560
BstAFI CTTAAG 1 cut(s) 62
BstAPI GCANNNNNTGC 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 119
BstC8I GCNNGC 3 cut(s) 43, 81, 298
BstDEI CTNAG 2 cut(s) 216, 377
BstEII GGTNACC 1 cut(s) 485
BstFNI CGCG 1 cut(s) 138
BstKTI GATC 3 cut(s) 88, 96, 100
BstMAI GTCTC 3 cut(s) 215, 255, 562
BstMBI GATC 3 cut(s) 85, 93, 97
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstPAI GACNNNNGTC 1 cut(s) 207
BstPI GGTNACC 1 cut(s) 485
BstSCI CCNGG 1 cut(s) 333
BstSFI CTRYAG 1 cut(s) 520
BstUI CGCG 1 cut(s) 138
BstV2I GAAGAC 1 cut(s) 380
BstZ17I GTATAC 1 cut(s) 508
Bsu15I ATCGAT 1 cut(s) 96
BsuTUI ATCGAT 1 cut(s) 96
Cac8I GCNNGC 3 cut(s) 43, 81, 298
Cfr13I GGNCC 1 cut(s) 14
ClaI ATCGAT 1 cut(s) 96
Csp6I GTAC 3 cut(s) 256, 282, 370
CviAII CATG 1 cut(s) 76
CviJI RGCY 3 cut(s) 41, 148, 330
CviKI_1 RGCY 3 cut(s) 41, 148, 330
CviQI GTAC 3 cut(s) 256, 282, 370
DdeI CTNAG 2 cut(s) 216, 377
DpnI GATC 3 cut(s) 87, 95, 99
DpnII GATC 3 cut(s) 85, 93, 97
DraI TTTAAA 1 cut(s) 589
Eam1104I CTCTTC 1 cut(s) 560
EarI CTCTTC 1 cut(s) 560
Eco130I CCWWGG 2 cut(s) 10, 392
Eco31I GGTCTC 1 cut(s) 215
Eco47I GGWCC 1 cut(s) 14
Eco57I CTGAAG 1 cut(s) 584
Eco91I GGTNACC 1 cut(s) 485
EcoO65I GGTNACC 1 cut(s) 485
EcoT14I CCWWGG 2 cut(s) 10, 392
ErhI CCWWGG 2 cut(s) 10, 392
FaeI CATG 1 cut(s) 79
FaiI YATR 9 cut(s) 77, 225, 249, 294, 360, 478, 508, 555, 655
FalI AAGNNNNNCTT 4 cut(s) 376, 408, 557, 589
FaqI GGGAC 2 cut(s) 350, 427
FatI CATG 1 cut(s) 75
FauI CCCGC 1 cut(s) 163
FblI GTMKAC 2 cut(s) 175, 507
Fnu4HI GCNGC 1 cut(s) 331
Fsp4HI GCNGC 1 cut(s) 331
FspBI CTAG 2 cut(s) 18, 353
GluI GCNGC 1 cut(s) 331
HapII CCGG 1 cut(s) 334
Hin1II CATG 1 cut(s) 79
HincII GTYRAC 1 cut(s) 176
HindII GTYRAC 1 cut(s) 176
HindIII AAGCTT 1 cut(s) 39
HinfI GANTC 5 cut(s) 184, 347, 470, 570, 628
HpaII CCGG 1 cut(s) 334
Hpy166II GTNNAC 4 cut(s) 176, 193, 304, 508
Hpy188I TCNGA 2 cut(s) 496, 564
Hpy188III TCNNGA 3 cut(s) 23, 160, 647
Hpy8I GTNNAC 4 cut(s) 176, 193, 304, 508
Hpy99I CGWCG 1 cut(s) 180
HpyAV CCTTC 2 cut(s) 501, 590
HpyCH4III ACNGT 2 cut(s) 70, 521
HpyCH4IV ACGT 2 cut(s) 533, 643
HpyCH4V TGCA 4 cut(s) 45, 133, 287, 296
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 2 cut(s) 216, 377
HpySE526I ACGT 2 cut(s) 533, 643
Hsp92II CATG 1 cut(s) 79
Kzo9I GATC 3 cut(s) 85, 93, 97
LmnI GCTCC 1 cut(s) 167
LweI GCATC 1 cut(s) 529
MaeI CTAG 2 cut(s) 18, 353
MaeII ACGT 2 cut(s) 533, 643
MaeIII GTNAC 1 cut(s) 485
MalI GATC 3 cut(s) 87, 95, 99
MbiI CCGCTC 1 cut(s) 170
MboI GATC 3 cut(s) 85, 93, 97
MboII GAAGA 2 cut(s) 380, 577
MluCI AATT 2 cut(s) 53, 154
MlyI GAGTC 3 cut(s) 479, 564, 622
MnlI CCTC 2 cut(s) 19, 374
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 5 cut(s) 63, 236, 576, 588, 639
MspCI CTTAAG 1 cut(s) 62
MspI CCGG 1 cut(s) 334
MspR9I CCNGG 1 cut(s) 335
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 1 cut(s) 293
NciI CCSGG 1 cut(s) 335
NdeII GATC 3 cut(s) 85, 93, 97
NlaIII CATG 1 cut(s) 79
NspV TTCGAA 1 cut(s) 119
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 2 cut(s) 184, 347
PflFI GACNNNGTC 1 cut(s) 18
PkrI GCNGC 1 cut(s) 332
Ple19I CGATCG 1 cut(s) 100
PleI GAGTC 3 cut(s) 478, 564, 622
PpsI GAGTC 3 cut(s) 478, 564, 622
PshAI GACNNNNGTC 1 cut(s) 207
PspEI GGTNACC 1 cut(s) 485
PspPI GGNCC 1 cut(s) 14
PsyI GACNNNGTC 1 cut(s) 18
PvuI CGATCG 1 cut(s) 100
RsaI GTAC 3 cut(s) 257, 283, 371
RsaNI GTAC 3 cut(s) 256, 282, 370
SalI GTCGAC 1 cut(s) 174
SaqAI TTAA 5 cut(s) 63, 236, 576, 588, 639
SatI GCNGC 1 cut(s) 331
Sau3AI GATC 3 cut(s) 85, 93, 97
Sau96I GGNCC 1 cut(s) 14
SchI GAGTC 3 cut(s) 479, 564, 622
ScrFI CCNGG 1 cut(s) 335
SetI ASST 8 cut(s) 19, 43, 192, 283, 527, 536, 601, 646
SfaNI GCATC 1 cut(s) 529
SfcI CTRYAG 1 cut(s) 520
SfuI TTCGAA 1 cut(s) 119
SinI GGWCC 1 cut(s) 14
SmlI CTYRAG 2 cut(s) 23, 62
SmoI CTYRAG 2 cut(s) 23, 62
Sse9I AATT 2 cut(s) 53, 154
SsiI CCGC 3 cut(s) 136, 170, 331
SspMI CTAG 2 cut(s) 18, 353
StyD4I CCNGG 1 cut(s) 333
StyI CCWWGG 2 cut(s) 10, 392
TaaI ACNGT 2 cut(s) 70, 521
TaiI ACGT 2 cut(s) 536, 646
TaqI TCGA 8 cut(s) 96, 100, 119, 175, 201, 468, 609, 631
TaqII GACCGA 1 cut(s) 197
TasI AATT 2 cut(s) 53, 154
TatI WGTACW 1 cut(s) 369
TauI GCSGC 1 cut(s) 333
TfiI GAWTC 2 cut(s) 184, 347
Tru1I TTAA 5 cut(s) 63, 236, 576, 588, 639
Tru9I TTAA 5 cut(s) 63, 236, 576, 588, 639
TspDTI ATGAA 1 cut(s) 104
Tth111I GACNNNGTC 1 cut(s) 18
Vha464I CTTAAG 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 14
XapI RAATTY 1 cut(s) 53
XmiI GTMKAC 2 cut(s) 175, 507
XmnI GAANNNNTTC 1 cut(s) 600
XspI CTAG 2 cut(s) 18, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.