MD15G1105400.v1.1
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0019553
Physical Location & Seq
Reverse (-)
7372369 .. 7373268
900 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1105400.v1.1.491

Sequence Viewer

Length: 900 bp
ATGGAGCCTTTGGTGGGTGGAAGGTTTCAACTTGTTCGAAAGATCGGTAGCGGATCGTTTGGACAGGTCTACCGGGGTAGTAATATTCAGACAAACGAGGAGGTTGCTATCAAGCTCGAAAGTGCCAAGACGAGGCATCCACAACTGCTGTATGAATCAAAGTTTTATAAAATACTACATGGAGGACCTGGAGTTCCAAACGTGAGATCGTTTGGCTCCGAAGGAGACTACAGTTTTCTTGTCATGGATTTCCTTGGACCTAGTCTTGAGGATTTATTCCAAGCTTGCAGTAAGAAATTCTCTCTTAAGACGGTTCTCATGCTCGCAGATCAAATGCTCGATCGAGTGGAGTTTGTTCATTCGAAATCAGTCCTGCACCGCGACATCAAGCCAGACAATTTCGTGATGGGAGCGGGTCGACGGGCGAATCAGGTTTACATCATCGACTTCGGCCTCGCTAAGAAGTACAGAAACTCTTCAACTCATCGGCATATTCCGTACAGAGACAATAAAAGTCTGGTAGGTACTGCAAGATATGCAAGCGTGAACAACCATCTTGGCATTGAACAAAGCCGCCGGGACGATTTGGAATCACTAGGATATGTGCTTATGTACTTTCTAAGAGGAAGTCTTCCTTGGCAGGGTGTGAAAGCAGGGACAAAAATGCAGAAAGATGAGAATATCGCAGAAAAGAAAATGTCAACTTCTATCGAGTCGCTATGCTCTGGTTACCCTTCCGAGTTTTCGGTATACTTCCATTACTGTAGGTCATTGACGTTTGATGCTAAACCAGATTATGCTTATCTGAAGAGACTCTTTAATGGCGTTTTTAAAAGGGAAGGTTTTCAGTTCGACTACGATTTTGATTGGACTCGATCGCTTAAACGTCTGGAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04392 map04011 map04392
Pfam Domains
Protein Families
Pkinase

Protein Analysis

300

Amino Acids

34.31

Weight (kDa)

9.35

Isoelectric Point (pI)

44.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 9 - 228 7.1e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 10 - 217 2.1e-16 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 119 - 206 1.5e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019553)

Species Orthologous Gene IDs
malus_domestica MD08G1125500.v1.1 MD15G1105400.v1.1
pyrus_communis pycom08g10460 pycom15g09560
rosa_laevigata RLG00000023050 RLG00000036380
rosa_roxburghii Rroxscaffold_7G00212790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 168
AccBSI CCGCTC 1 cut(s) 413
AccI GTMKAC 3 cut(s) 69, 418, 750
AccII CGCG 1 cut(s) 381
AciI CCGC 4 cut(s) 51, 379, 413, 574
AclWI GGATC 1 cut(s) 61
AcsI RAATTY 1 cut(s) 296
AcuI CTGAAG 1 cut(s) 827
AfaI GTAC 4 cut(s) 467, 500, 526, 614
AfiI CCNNNNNNNGG 3 cut(s) 14, 132, 641
AflII CTTAAG 1 cut(s) 305
AgsI TTSAA 3 cut(s) 29, 480, 566
AjnI CCWGG 1 cut(s) 187
AjuI GAANNNNNNNTTGG 2 cut(s) 619, 651
AloI GAACNNNNNNTCC 2 cut(s) 177, 209
AluBI AGCT 2 cut(s) 115, 284
AluI AGCT 2 cut(s) 115, 284
Alw26I GTCTC 3 cut(s) 219, 498, 805
AlwI GGATC 1 cut(s) 61
AoxI GGCC 1 cut(s) 451
ApoI RAATTY 1 cut(s) 296
ArsI GACNNNNNNTTYG 2 cut(s) 845, 877
Asp700I GAANNNNTTC 2 cut(s) 475, 843
AspS9I GGNCC 2 cut(s) 185, 257
AsuC2I CCSGG 2 cut(s) 74, 578
AsuII TTCGAA 2 cut(s) 37, 362
AvaII GGWCC 2 cut(s) 185, 257
BbsI GAAGAC 1 cut(s) 623
BccI CCATC 2 cut(s) 400, 561
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 1 cut(s) 189
BcnI CCSGG 2 cut(s) 74, 578
BcoDI GTCTC 3 cut(s) 219, 498, 805
BfaI CTAG 2 cut(s) 261, 596
BfmI CTRYAG 2 cut(s) 229, 763
BfrI CTTAAG 1 cut(s) 305
BisI GCNGC 1 cut(s) 574
BlsI GCNGC 1 cut(s) 575
Bme1390I CCNGG 3 cut(s) 74, 189, 578
Bme18I GGWCC 2 cut(s) 185, 257
BmgT120I GGNCC 2 cut(s) 185, 257
BmiI GGNNCC 2 cut(s) 6, 217
BmrFI CCNGG 3 cut(s) 74, 189, 578
BmsI GCATC 2 cut(s) 145, 772
BpiI GAAGAC 1 cut(s) 623
BpmI CTGGAG 1 cut(s) 210
Bpu14I TTCGAA 2 cut(s) 37, 362
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 2 cut(s) 74, 578
BsaJI CCNNGG 3 cut(s) 73, 253, 635
Bsc4I CCNNNNNNNGG 3 cut(s) 14, 132, 641
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 3 cut(s) 73, 253, 635
BseGI GGATG 1 cut(s) 136
BseLI CCNNNNNNNGG 3 cut(s) 14, 132, 641
BseRI GAGGAG 1 cut(s) 113
BsgI GTGCAG 1 cut(s) 359
Bsh1236I CGCG 1 cut(s) 381
Bsh1285I CGRYCG 2 cut(s) 343, 878
BshFI GGCC 1 cut(s) 453
BsiEI CGRYCG 2 cut(s) 343, 878
BsiSI CCGG 2 cut(s) 73, 577
BslFI GGGAC 2 cut(s) 593, 670
BslI CCNNNNNNNGG 3 cut(s) 14, 132, 641
BsmAI GTCTC 3 cut(s) 219, 498, 805
BsmFI GGGAC 2 cut(s) 593, 670
BsnI GGCC 1 cut(s) 453
Bsp119I TTCGAA 2 cut(s) 37, 362
Bsp143I GATC 6 cut(s) 42, 53, 206, 328, 340, 875
BspACI CCGC 4 cut(s) 51, 379, 413, 574
BspANI GGCC 1 cut(s) 453
BspFNI CGCG 1 cut(s) 381
BspLI GGNNCC 2 cut(s) 6, 217
BspPI GGATC 1 cut(s) 61
BspT104I TTCGAA 2 cut(s) 37, 362
BspTI CTTAAG 1 cut(s) 305
BsrBI CCGCTC 1 cut(s) 413
BssECI CCNNGG 3 cut(s) 73, 253, 635
BssMI GATC 6 cut(s) 42, 53, 206, 328, 340, 875
BssNAI GTATAC 1 cut(s) 751
BssT1I CCWWGG 2 cut(s) 253, 635
Bst1107I GTATAC 1 cut(s) 751
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 3 cut(s) 233, 313, 764
Bst6I CTCTTC 2 cut(s) 481, 803
BstAFI CTTAAG 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 536
BstBI TTCGAA 2 cut(s) 37, 362
BstC8I GCNNGC 3 cut(s) 286, 324, 541
BstDEI CTNAG 2 cut(s) 459, 620
BstEII GGTNACC 1 cut(s) 728
BstF5I GGATG 1 cut(s) 136
BstFNI CGCG 1 cut(s) 381
BstKTI GATC 6 cut(s) 45, 56, 209, 331, 343, 878
BstMAI GTCTC 3 cut(s) 219, 498, 805
BstMBI GATC 6 cut(s) 42, 53, 206, 328, 340, 875
BstMCI CGRYCG 2 cut(s) 343, 878
BstMWI GCNNNNNNNGC 1 cut(s) 536
BstNI CCWGG 1 cut(s) 189
BstPI GGTNACC 1 cut(s) 728
BstSCI CCNGG 3 cut(s) 72, 187, 576
BstSFI CTRYAG 2 cut(s) 229, 763
BstUI CGCG 1 cut(s) 381
BstV2I GAAGAC 1 cut(s) 623
BstZ17I GTATAC 1 cut(s) 751
BsuRI GGCC 1 cut(s) 453
BtsCI GGATG 1 cut(s) 136
Cac8I GCNNGC 3 cut(s) 286, 324, 541
Cfr13I GGNCC 2 cut(s) 185, 257
Csp6I GTAC 4 cut(s) 466, 499, 525, 613
CviAII CATG 3 cut(s) 179, 244, 319
CviJI RGCY 7 cut(s) 7, 115, 216, 284, 391, 453, 573
CviKI_1 RGCY 7 cut(s) 7, 115, 216, 284, 391, 453, 573
CviQI GTAC 4 cut(s) 466, 499, 525, 613
DdeI CTNAG 2 cut(s) 459, 620
DpnI GATC 6 cut(s) 44, 55, 208, 330, 342, 877
DpnII GATC 6 cut(s) 42, 53, 206, 328, 340, 875
DraI TTTAAA 1 cut(s) 832
Eam1104I CTCTTC 2 cut(s) 481, 803
EarI CTCTTC 2 cut(s) 481, 803
Eco130I CCWWGG 2 cut(s) 253, 635
Eco47I GGWCC 2 cut(s) 185, 257
Eco57I CTGAAG 1 cut(s) 827
Eco91I GGTNACC 1 cut(s) 728
EcoO109I RGGNCCY 1 cut(s) 185
EcoO65I GGTNACC 1 cut(s) 728
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 2 cut(s) 253, 635
ErhI CCWWGG 2 cut(s) 253, 635
FaeI CATG 3 cut(s) 182, 247, 322
FalI AAGNNNNNCTT 4 cut(s) 619, 651, 800, 832
FaqI GGGAC 2 cut(s) 593, 670
FatI CATG 3 cut(s) 178, 243, 318
FauI CCCGC 1 cut(s) 406
FblI GTMKAC 3 cut(s) 69, 418, 750
Fnu4HI GCNGC 1 cut(s) 574
FokI GGATG 1 cut(s) 123
Fsp4HI GCNGC 1 cut(s) 574
FspBI CTAG 2 cut(s) 261, 596
GluI GCNGC 1 cut(s) 574
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 1 cut(s) 453
HapII CCGG 2 cut(s) 73, 577
Hin1II CATG 3 cut(s) 182, 247, 322
HincII GTYRAC 2 cut(s) 419, 702
HindII GTYRAC 2 cut(s) 419, 702
HindIII AAGCTT 1 cut(s) 282
HinfI GANTC 6 cut(s) 155, 427, 590, 713, 813, 871
HpaII CCGG 2 cut(s) 73, 577
Hpy166II GTNNAC 6 cut(s) 70, 419, 436, 547, 702, 751
Hpy188I TCNGA 4 cut(s) 90, 220, 739, 807
Hpy188III TCNNGA 3 cut(s) 266, 403, 890
Hpy8I GTNNAC 6 cut(s) 70, 419, 436, 547, 702, 751
Hpy99I CGWCG 1 cut(s) 423
HpyAV CCTTC 4 cut(s) 15, 215, 744, 833
HpyCH4III ACNGT 3 cut(s) 233, 313, 764
HpyCH4IV ACGT 3 cut(s) 201, 776, 886
HpyCH4V TGCA 5 cut(s) 288, 376, 530, 539, 667
HpyF10VI GCNNNNNNNGC 1 cut(s) 536
HpyF3I CTNAG 2 cut(s) 459, 620
HpySE526I ACGT 3 cut(s) 201, 776, 886
Hsp92II CATG 3 cut(s) 182, 247, 322
Kzo9I GATC 6 cut(s) 42, 53, 206, 328, 340, 875
LmnI GCTCC 3 cut(s) 4, 221, 410
LweI GCATC 2 cut(s) 145, 772
MaeI CTAG 2 cut(s) 261, 596
MaeII ACGT 3 cut(s) 201, 776, 886
MaeIII GTNAC 1 cut(s) 728
MalI GATC 6 cut(s) 44, 55, 208, 330, 342, 877
MbiI CCGCTC 1 cut(s) 413
MboI GATC 6 cut(s) 42, 53, 206, 328, 340, 875
MboII GAAGA 3 cut(s) 468, 623, 820
MluCI AATT 2 cut(s) 296, 397
MlyI GAGTC 3 cut(s) 722, 807, 865
MnlI CCTC 7 cut(s) 91, 94, 126, 176, 262, 464, 617
MroXI GAANNNNTTC 2 cut(s) 475, 843
MseI TTAA 4 cut(s) 306, 819, 831, 882
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 2 cut(s) 73, 577
MspR9I CCNGG 3 cut(s) 74, 189, 578
MvaI CCWGG 1 cut(s) 189
MvnI CGCG 1 cut(s) 381
MwoI GCNNNNNNNGC 1 cut(s) 536
NciI CCSGG 2 cut(s) 74, 578
NdeII GATC 6 cut(s) 42, 53, 206, 328, 340, 875
NlaIII CATG 3 cut(s) 182, 247, 322
NlaIV GGNNCC 2 cut(s) 6, 217
NspV TTCGAA 2 cut(s) 37, 362
PdmI GAANNNNTTC 2 cut(s) 475, 843
PfeI GAWTC 3 cut(s) 155, 427, 590
PflFI GACNNNGTC 1 cut(s) 261
PkrI GCNGC 1 cut(s) 575
Ple19I CGATCG 2 cut(s) 343, 878
PleI GAGTC 3 cut(s) 721, 807, 865
PpsI GAGTC 3 cut(s) 721, 807, 865
PpuMI RGGWCCY 1 cut(s) 185
PsiI TTATAA 1 cut(s) 168
Psp5II RGGWCCY 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 187
PspEI GGTNACC 1 cut(s) 728
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 6, 217
PspPI GGNCC 2 cut(s) 185, 257
PspPPI RGGWCCY 1 cut(s) 185
PsyI GACNNNGTC 1 cut(s) 261
PvuI CGATCG 2 cut(s) 343, 878
RsaI GTAC 4 cut(s) 467, 500, 526, 614
RsaNI GTAC 4 cut(s) 466, 499, 525, 613
SalI GTCGAC 1 cut(s) 417
SaqAI TTAA 4 cut(s) 306, 819, 831, 882
SatI GCNGC 1 cut(s) 574
Sau3AI GATC 6 cut(s) 42, 53, 206, 328, 340, 875
Sau96I GGNCC 2 cut(s) 185, 257
SchI GAGTC 3 cut(s) 722, 807, 865
ScrFI CCNGG 3 cut(s) 74, 189, 578
SfaNI GCATC 2 cut(s) 145, 772
SfcI CTRYAG 2 cut(s) 229, 763
SfuI TTCGAA 2 cut(s) 37, 362
SinI GGWCC 2 cut(s) 185, 257
SmlI CTYRAG 2 cut(s) 266, 305
SmoI CTYRAG 2 cut(s) 266, 305
Sse9I AATT 2 cut(s) 296, 397
SsiI CCGC 4 cut(s) 51, 379, 413, 574
SspI AATATT 1 cut(s) 85
SspMI CTAG 2 cut(s) 261, 596
StyD4I CCNGG 3 cut(s) 72, 187, 576
StyI CCWWGG 2 cut(s) 253, 635
TaaI ACNGT 3 cut(s) 233, 313, 764
TaiI ACGT 3 cut(s) 204, 779, 889
TasI AATT 2 cut(s) 296, 397
TatI WGTACW 2 cut(s) 465, 612
TauI GCSGC 1 cut(s) 576
TfiI GAWTC 3 cut(s) 155, 427, 590
Tru1I TTAA 4 cut(s) 306, 819, 831, 882
Tru9I TTAA 4 cut(s) 306, 819, 831, 882
TspDTI ATGAA 2 cut(s) 168, 347
TspGWI ACGGA 1 cut(s) 486
Tth111I GACNNNGTC 1 cut(s) 261
Vha464I CTTAAG 1 cut(s) 305
VpaK11BI GGWCC 2 cut(s) 185, 257
XapI RAATTY 1 cut(s) 296
XmiI GTMKAC 3 cut(s) 69, 418, 750
XmnI GAANNNNTTC 2 cut(s) 475, 843
XspI CTAG 2 cut(s) 261, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.