Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0014333

Physical Location & Seq

Genomic Coordinates Forward (+)

19186900 .. 19189581

Gene Structure 2682 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1162900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2682 bp

ATGGCAGCTGAAATTTCGTCCGGCAGCCGAAGAAATTCCAACACTCAGCTCCTGGAAGAGCTCGAAGCACTGAGTGAGTCACTCTACACATCCCACACTTCGAGCACTAGTACTCGAAGAACTGCCTCCCTTGTCCTTCCTCGAAGCTCGGTTCCTCCCATACCATCTAAAGATGGAGTTGTTCCGCCGAGTGTTGAGGAGATTAGACTCAATAACAAACCGCGAAGGCGGATGTCTTTGTCCCCATGGCGCTCCCGCCCAAAACTTACTAATGATGAGGAAGATGAGCATAAGGATCAAGGCAAGAAGGCCGTGAAATATAATTCACCGGAGCTAAGAAGCTTGGATGACAAGGCAACTGCCATCATAGAGAGAAAAGGTATCTGGAATTGGAAGCCGGTTCGCGCTATTTCCCACATTGGAATGCAGAAGCTGAGCTGCTTATTTTCTGTTGAAGTTGTCACTGCACAAGGCCTTCCAACTTCAATGAATGGCCTTAGACTTTCTGTCTGCGTTAGAAAGAAGGAAACAAAAGACGGAGCAGTTCAAACAATGCCGTCAAGGGTGACACAAGGAGCTGCTGATTTCGAAGAGACCCTTTTCGTTCGGTGCCATGTTTATTGCAGCTCCGCCCATGGAAAAAAGACAAAGTTTGAGCCGAGACCCTTTTGGGTATATTTGTTTGCAGTTGATGCAGAGGAGCTTGATTTTGGGAGAAGTACTGTGGATTTGAGTCAGATGATTCAGGAGTCCATTGAGAAGAGCCGTGAAGGCCAACGAATTCGACAATGGGATACGAGTTTTAAACTGTTAGGGAAGGCGAAAGGCGGTGAGCTTGCTCTGAAACTAGGGTTTCAGATTATGGAGAAGGATGGAGGAGTTGGGATTTACAGTCAGGTTGAGGATTTGAAGTCTAGTAAGTCCAACAATTTCACATCTTCTTTTGGTAGGAAGCAATCGAAAACATCATTTAGTGTGCCTAGTCCAAAACTTTCAAGCAGAGGAGAAGCTTGGACACCTTCACAAGCAAGAAAAGCAGTTGATCTTCAGGGGATTGATGAACTCAACCTTGATGAACCGAACCCGGTTCCTATATCATCACCTTCTTCTTCTTCTGCAGCTCAAAAGCCTAAAGAACCAGAAGTGCCAAAGGTGGAGGAGCTTGATCTGCCAGACTTTGAGGTAGTGGATAAAGGAGTTGAATTCCAAGACAAGGGAAAGGAATATGGGGAGGAGCAATCCGAAAAGTCTGTTGGCGAAAAATCAGCAACATCAAGTGAGGTTGTGAAGGAAATTGTGCAAGACCATGTTCATACAACAAGATTGACAGAACTTGATTCAATTGCTCAGCAGATTAAGGCTCTTGAGTCTTTGATGGGGAAAGAAAAAATCGACGAAAAAGATGAAGAGGATGAAGATATCAAATCACAAAAATTGGAGGCAGATGAAGAAAATGTGACAAAGGAGTTTCTTCAAATGCTTGAGGAGGAGGAGATCCTAAACGAATACAAATTGAATCAAAGTGATGTTCCACCTTTTGCCCTTGAAGGGGCAGAGGAATCTGGTGAGGGTGAAGCAGCAGCAGTTTACCTTCCTGATCTCGGAAAGAGCTTAGGGAGTGTGGTTCAAACCCGCGATGGAGGATATTTGGCATCCATGAATCCTTTTGATACTTTGGTAGCAAGGAAAGACACTCCAAAACTTGCCATGCAGATATCAAAGCCGTTTGTTCTGCCATGGGATCAGTCCATGAGTGGATTTGAATTGTTTCAAAGGATTGCAGGTATTGGACTTGATGAGCTGAACTCCCAGATTATGAATCTGATGGCATTGGATGAATTGATGGATAAAACTGCGGAGCAGATTGCTTTTGAAGGCATTGCGTCTGCAATCATTCAAGGAAGGAACAAAGAAGGTGCTAGCTCGAGCGCTGCAAGAACAATTGCTGCTGTTAAAACCATGGCAAATACATTGAGCACAGGCAGGAAGGAGAGGATTTCGACCGGGATTTGGAATGTGAACGAGAACCCTTTGACACTAGAGGAGATTCTAGCCTTTTCCATGCAGAAAATCGAGGCCATGGCGCTCGAAGCTTTGAAAATTCAAGCTGAAATGGCTGAGGAAGAAGCCCCATTTGAAGTTTCACCAACGAACAATAGTTTCACAAATTCAAGTGGTGCAAAAGTACTTCAAAACCACCCTCTCGCTTCTTCAATATCGCTCGAGGATTGGATCAAAAACCACAGCCTGGCAAGTTCAGAAGGTTTACAAGACGAAAACCAGCCGGAGACAATTACACTGGCTGTGATTGTTCAGCTGAGGGATCCCTTAAGGCGATATGAGGCGGTTGGAGGCCCTATGATAGCGCTTATTTACGCAACGAGAGCTGATGGCGCCGTCAATGAGGAGGAGAAGAGATTCAGGGTGACAAGCATGCATGTGGGAGGATTGAAAGTGAGGACAAAAGGGGGAAAGAGGAATGCGTGGGACAGTGAGAGGCAAAGGCTAACTGCAATGCAGTGGCTAATTTCTTATGGATTGGCGAAAGCTGGTGCGAGGAAGAAGGGCAAACATCATGTGGCATCCAAAGGTCAGGATTTGTTGTGGAGCATTTCATCAAGAATGATGGCTAATATGTGGCTCAAGTATATGAGAAATCCAGATGTAAAATTTACCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006810 GO:0006928 GO:0006950 GO:0006970 GO:0006996 GO:0007097 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009657 GO:0009658 GO:0009787 GO:0009902 GO:0009903 GO:0009966 GO:0009987 GO:0010029 GO:0010646 GO:0016020 GO:0016043 GO:0019750 GO:0023051 GO:0030029 GO:0030048 GO:0030705 GO:0031022 GO:0042592 GO:0044424 GO:0044444 GO:0044464 GO:0046907 GO:0048580 GO:0048583 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051640 GO:0051641 GO:0051644 GO:0051647 GO:0051649 GO:0051656 GO:0051667 GO:0055081 GO:0055088 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0099515 GO:1900140 GO:1901419 GO:1902265 GO:1905957 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NT-C2

Protein Analysis

894

Amino Acids

99.1

Weight (kDa)

5.62

Isoelectric Point (pI)

47.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NT-C2	PF10358	142 - 291	2.9e-13	N-terminal C2 in EEIG1 and EHBP1 proteins
PMI1_PMIR1-2_C	PF21745	567 - 715	3.5e-59	PMI1/PMIR1-2, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42550
fragaria_vesca	FvH4_2g26870
malus_domestica	MD08G1162900.v1.1
prunus_persica	Prupe.1G498000_v2.0.a1
pyrus_communis	pycom08g14070 pycom15g31260
rosa_chinensis	RchiOBHm_Chr6g0295721
rosa_laevigata	RLG00000011772
rosa_multiflora	Rmu_sc0007372.1_g000001 Rmu_sc0007986.1_g000004
rosa_roxburghii	Rroxscaffold_7G00172180
rosa_rugosa	Rorug06G0253800
rosa_samantha	Rh6AG367200 Rh6BG375000 Rh6CG381300 Rh6DG368100
rosa_wichuraiana	Rw6G032050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1772
AccB1I	GGYRCC	2 cut(s)	609, 2393
AccB7I	CCANNNNNTGG	1 cut(s)	2248
AccII	CGCG	3 cut(s)	223, 405, 1635
AciI	CCGC	9 cut(s)	185, 221, 229, 256, 630, 828, 1633, 1856, 2345
AclWI	GGATC	6 cut(s)	303, 1489, 1749, 2240, 2318, 2331
AcsI	RAATTY	7 cut(s)	12, 34, 780, 1202, 2100, 2167, 2669
AcuI	CTGAAG	1 cut(s)	1031
AcyI	GRCGYC	1 cut(s)	2394
AdeI	CACNNNGTG	1 cut(s)	74
AfaI	GTAC	3 cut(s)	112, 721, 2187
AfeI	AGCGCT	2 cut(s)	1930, 2367
AfiI	CCNNNNNNNGG	6 cut(s)	1213, 1548, 1549, 1601, 2010, 2248
AflII	CTTAAG	1 cut(s)	2329
AhdI	GACNNNNNGTC	1 cut(s)	506
AhlI	ACTAGT	1 cut(s)	107
AjnI	CCWGG	2 cut(s)	51, 2247
AjuI	GAANNNNNNNTTGG	2 cut(s)	1236, 1268
Alw21I	GWGCWC	3 cut(s)	63, 107, 1979
Alw26I	GTCTC	3 cut(s)	587, 655, 2282
AlwI	GGATC	6 cut(s)	303, 1489, 1749, 2240, 2318, 2331
AlwNI	CAGNNNCTG	2 cut(s)	52, 433
Ama87I	CYCGRG	2 cut(s)	1924, 2222
Aor51HI	AGCGCT	2 cut(s)	1930, 2367
AoxI	GGCC	6 cut(s)	309, 472, 493, 772, 2076, 2353
ApoI	RAATTY	7 cut(s)	12, 34, 780, 1202, 2100, 2167, 2669
Asp700I	GAANNNNTTC	3 cut(s)	34, 1767, 2417
AspLEI	GCGC	6 cut(s)	252, 407, 1931, 2086, 2368, 2396
AspS9I	GGNCC	1 cut(s)	2354
AsuC2I	CCSGG	2 cut(s)	1085, 2005
AsuHPI	GGTGA	8 cut(s)	318, 577, 842, 1092, 1577, 1583, 2136, 2437
AsuII	TTCGAA	1 cut(s)	588
AsuNHI	GCTAGC	1 cut(s)	1919
AvaI	CYCGRG	2 cut(s)	1924, 2222
BaeI	ACNNNNGTAYC	2 cut(s)	1662, 1695
BamHI	GGATCC	1 cut(s)	2323
BanI	GGYRCC	2 cut(s)	609, 2393
BanII	GRGCYC	1 cut(s)	63
Bbv12I	GWGCWC	3 cut(s)	63, 107, 1979
BbvCI	CCTCAGC	2 cut(s)	2118, 2318
BceAI	ACGGC	5 cut(s)	296, 541, 750, 1708, 2381
BciT130I	CCWGG	2 cut(s)	53, 2249
BciVI	GTATCC	1 cut(s)	787
BcnI	CCSGG	2 cut(s)	1085, 2005
BcoDI	GTCTC	3 cut(s)	587, 655, 2282
BcuI	ACTAGT	1 cut(s)	107
BfaI	CTAG	7 cut(s)	108, 848, 915, 981, 1920, 2039, 2051
BfmI	CTRYAG	1 cut(s)	1116
BfoI	RGCGCY	5 cut(s)	253, 1932, 2087, 2369, 2397
BfrI	CTTAAG	1 cut(s)	2329
BfuAI	ACCTGC	1 cut(s)	1772
BfuI	GTATCC	1 cut(s)	787
BglI	GCCNNNNNGGC	1 cut(s)	771
BlpI	GCTNAGC	2 cut(s)	434, 1347
BmcAI	AGTACT	3 cut(s)	112, 721, 2187
Bme1390I	CCNGG	4 cut(s)	53, 1085, 2005, 2249
BmeRI	GACNNNNNGTC	1 cut(s)	506
BmeT110I	CYCGRG	2 cut(s)	1924, 2222
BmgT120I	GGNCC	1 cut(s)	2354
BmiI	GGNNCC	5 cut(s)	153, 611, 1089, 2325, 2395
BmrFI	CCNGG	4 cut(s)	53, 1085, 2005, 2249
BmsI	GCATC	3 cut(s)	682, 1661, 2591
BmtI	GCTAGC	1 cut(s)	1923
BplI	GAGNNNNNCTC	2 cut(s)	1790, 1822
Bpu10I	CCTNAGC	3 cut(s)	1612, 2118, 2318
Bpu1102I	GCTNAGC	2 cut(s)	434, 1347
Bpu14I	TTCGAA	1 cut(s)	588
BpuEI	CTTGAG	3 cut(s)	1385, 1502, 2627
BpuMI	CCSGG	2 cut(s)	1085, 2005
BsaHI	GRCGYC	1 cut(s)	2394
BsaI	GGTCTC	2 cut(s)	587, 655
BsaJI	CCNNGG	5 cut(s)	245, 634, 1736, 1959, 2079
BsaWI	WCCGGW	1 cut(s)	328
Bsc4I	CCNNNNNNNGG	6 cut(s)	1213, 1548, 1549, 1601, 2010, 2248
Bse118I	RCCGGY	1 cut(s)	397
Bse1I	ACTGG	1 cut(s)	2304
Bse3DI	GCAATG	2 cut(s)	1878, 2520
BseBI	CCWGG	2 cut(s)	53, 2249
BseDI	CCNNGG	5 cut(s)	245, 634, 1736, 1959, 2079
BseGI	GGATG	8 cut(s)	89, 237, 352, 877, 1417, 1652, 1840, 2582
BseLI	CCNNNNNNNGG	6 cut(s)	1213, 1548, 1549, 1601, 2010, 2248
BseMI	GCAATG	2 cut(s)	1878, 2520
BseMII	CTCAG	6 cut(s)	59, 62, 425, 1361, 2109, 2309
BseNI	ACTGG	1 cut(s)	2304
BsgI	GTGCAG	1 cut(s)	450
Bsh1236I	CGCG	3 cut(s)	223, 405, 1635
Bsh1285I	CGRYCG	1 cut(s)	2004
BshFI	GGCC	6 cut(s)	311, 474, 495, 774, 2078, 2355
BshNI	GGYRCC	2 cut(s)	609, 2393
BsiEI	CGRYCG	1 cut(s)	2004
BsiHKAI	GWGCWC	3 cut(s)	63, 107, 1979
BsiHKCI	CYCGRG	2 cut(s)	1924, 2222
BsiSI	CCGG	6 cut(s)	21, 329, 398, 1085, 2004, 2285
BslFI	GGGAC	2 cut(s)	226, 2501
BslI	CCNNNNNNNGG	6 cut(s)	1213, 1548, 1549, 1601, 2010, 2248
BsmAI	GTCTC	3 cut(s)	587, 655, 2282
BsmFI	GGGAC	2 cut(s)	226, 2501
BsmI	GAATGC	2 cut(s)	429, 2485
BsnI	GGCC	6 cut(s)	311, 474, 495, 774, 2078, 2355
Bso31I	GGTCTC	2 cut(s)	587, 655
BsoBI	CYCGRG	2 cut(s)	1924, 2222
Bsp119I	TTCGAA	1 cut(s)	588
Bsp1286I	GDGCHC	3 cut(s)	63, 107, 1979
Bsp143I	GATC	8 cut(s)	295, 1042, 1165, 1494, 1597, 1741, 2232, 2323
Bsp1720I	GCTNAGC	2 cut(s)	434, 1347
Bsp19I	CCATGG	5 cut(s)	245, 634, 1736, 1959, 2079
BspACI	CCGC	9 cut(s)	185, 221, 229, 256, 630, 828, 1633, 1856, 2345
BspANI	GGCC	6 cut(s)	311, 474, 495, 774, 2078, 2355
BspCNI	CTCAG	6 cut(s)	58, 63, 426, 1360, 2110, 2310
BspFNI	CGCG	3 cut(s)	223, 405, 1635
BspLI	GGNNCC	5 cut(s)	153, 611, 1089, 2325, 2395
BspMAI	CTGCAG	1 cut(s)	1120
BspMI	ACCTGC	1 cut(s)	1772
BspOI	GCTAGC	1 cut(s)	1923
BspPI	GGATC	6 cut(s)	303, 1489, 1749, 2240, 2318, 2331
BspQI	GCTCTTC	2 cut(s)	51, 755
BspT104I	TTCGAA	1 cut(s)	588
BspT107I	GGYRCC	2 cut(s)	609, 2393
BspTI	CTTAAG	1 cut(s)	2329
BspTNI	GGTCTC	2 cut(s)	587, 655
BsrDI	GCAATG	2 cut(s)	1878, 2520
BsrFI	RCCGGY	1 cut(s)	397
BsrI	ACTGG	1 cut(s)	2304
BssAI	RCCGGY	1 cut(s)	397
BssECI	CCNNGG	5 cut(s)	245, 634, 1736, 1959, 2079
BssMI	GATC	8 cut(s)	295, 1042, 1165, 1494, 1597, 1741, 2232, 2323
BssNI	GRCGYC	1 cut(s)	2394
BssT1I	CCWWGG	5 cut(s)	245, 634, 1736, 1959, 2079
Bst2UI	CCWGG	2 cut(s)	53, 2249
Bst4CI	ACNGT	4 cut(s)	724, 810, 893, 2492
Bst6I	CTCTTC	5 cut(s)	51, 585, 755, 1401, 2408
BstACI	GRCGYC	1 cut(s)	2394
BstAFI	CTTAAG	1 cut(s)	2329
BstAPI	GCANNNNNTGC	1 cut(s)	692
BstBI	TTCGAA	1 cut(s)	588
BstC8I	GCNNGC	3 cut(s)	837, 1921, 2435
BstDEI	CTNAG	9 cut(s)	45, 71, 335, 434, 497, 1347, 1612, 2118, 2318
BstDSI	CCRYGG	5 cut(s)	245, 634, 1736, 1959, 2079
BstF5I	GGATG	8 cut(s)	89, 237, 352, 877, 1417, 1652, 1840, 2582
BstFNI	CGCG	3 cut(s)	223, 405, 1635
BstH2I	RGCGCY	5 cut(s)	253, 1932, 2087, 2369, 2397
BstHHI	GCGC	6 cut(s)	252, 407, 1931, 2086, 2368, 2396
BstKTI	GATC	8 cut(s)	298, 1045, 1168, 1497, 1600, 1744, 2235, 2326
BstMAI	GTCTC	3 cut(s)	587, 655, 2282
BstMBI	GATC	8 cut(s)	295, 1042, 1165, 1494, 1597, 1741, 2232, 2323
BstMCI	CGRYCG	1 cut(s)	2004
BstMWI	GCNNNNNNNGC	8 cut(s)	692, 771, 1034, 1168, 2090, 2114, 2384, 2393
BstNI	CCWGG	2 cut(s)	53, 2249
BstNSI	RCATGY	2 cut(s)	2437, 2441
BstSCI	CCNGG	4 cut(s)	51, 1083, 2003, 2247
BstSFI	CTRYAG	1 cut(s)	1116
BstUI	CGCG	3 cut(s)	223, 405, 1635
BstX2I	RGATCY	2 cut(s)	1494, 2323
BstYI	RGATCY	2 cut(s)	1494, 2323
BsuI	GTATCC	1 cut(s)	787
BsuRI	GGCC	6 cut(s)	311, 474, 495, 774, 2078, 2355
BtgI	CCRYGG	5 cut(s)	245, 634, 1736, 1959, 2079
BtgZI	GCGATG	1 cut(s)	1650
BtsCI	GGATG	8 cut(s)	89, 237, 352, 877, 1417, 1652, 1840, 2582
BtsI	GCAGTG	2 cut(s)	462, 2525
BtsIMutI	CAGTG	5 cut(s)	68, 462, 2297, 2497, 2525
BveI	ACCTGC	1 cut(s)	1772
Cac8I	GCNNGC	3 cut(s)	837, 1921, 2435
CaiI	CAGNNNCTG	2 cut(s)	52, 433
CfoI	GCGC	6 cut(s)	252, 407, 1931, 2086, 2368, 2396
Cfr10I	RCCGGY	1 cut(s)	397
Cfr13I	GGNCC	1 cut(s)	2354
CseI	GACGC	1 cut(s)	1872
Csp6I	GTAC	3 cut(s)	111, 720, 2186
CviQI	GTAC	3 cut(s)	111, 720, 2186
DdeI	CTNAG	9 cut(s)	45, 71, 335, 434, 497, 1347, 1612, 2118, 2318
DinI	GGCGCC	1 cut(s)	2395
DpnI	GATC	8 cut(s)	297, 1044, 1167, 1496, 1599, 1743, 2234, 2325
DpnII	GATC	8 cut(s)	295, 1042, 1165, 1494, 1597, 1741, 2232, 2323
DraI	TTTAAA	1 cut(s)	805
DraIII	CACNNNGTG	1 cut(s)	74
DriI	GACNNNNNGTC	1 cut(s)	506
Eam1104I	CTCTTC	5 cut(s)	51, 585, 755, 1401, 2408
Eam1105I	GACNNNNNGTC	1 cut(s)	506
EarI	CTCTTC	5 cut(s)	51, 585, 755, 1401, 2408
EciI	GGCGGA	3 cut(s)	174, 244, 619
Ecl136II	GAGCTC	1 cut(s)	61
Eco130I	CCWWGG	5 cut(s)	245, 634, 1736, 1959, 2079
Eco147I	AGGCCT	1 cut(s)	474
Eco24I	GRGCYC	1 cut(s)	63
Eco31I	GGTCTC	2 cut(s)	587, 655
Eco32I	GATATC	2 cut(s)	1420, 1716
Eco47III	AGCGCT	2 cut(s)	1930, 2367
Eco53kI	GAGCTC	1 cut(s)	61
Eco57I	CTGAAG	1 cut(s)	1031
Eco88I	CYCGRG	2 cut(s)	1924, 2222
EcoICRI	GAGCTC	1 cut(s)	61
EcoO109I	RGGNCCY	1 cut(s)	2354
EcoRI	GAATTC	2 cut(s)	780, 1202
EcoRII	CCWGG	2 cut(s)	51, 2247
EcoRV	GATATC	2 cut(s)	1420, 1716
EcoT14I	CCWWGG	5 cut(s)	245, 634, 1736, 1959, 2079
EcoT22I	ATGCAT	1 cut(s)	2439
EcoT38I	GRGCYC	1 cut(s)	63
EgeI	GGCGCC	1 cut(s)	2395
EheI	GGCGCC	1 cut(s)	2395
ErhI	CCWWGG	5 cut(s)	245, 634, 1736, 1959, 2079
FalI	AAGNNNNNCTT	2 cut(s)	582, 614
FaqI	GGGAC	2 cut(s)	226, 2501
FauI	CCCGC	2 cut(s)	263, 1640
FokI	GGATG	8 cut(s)	76, 244, 359, 884, 1424, 1639, 1847, 2569
FriOI	GRGCYC	1 cut(s)	63
FspBI	CTAG	7 cut(s)	108, 848, 915, 981, 1920, 2039, 2051
GlaI	GCGC	6 cut(s)	251, 406, 1930, 2085, 2367, 2395
HaeII	RGCGCY	5 cut(s)	253, 1932, 2087, 2369, 2397
HaeIII	GGCC	6 cut(s)	311, 474, 495, 774, 2078, 2355
HapII	CCGG	6 cut(s)	21, 329, 398, 1085, 2004, 2285
HgaI	GACGC	1 cut(s)	1872
HhaI	GCGC	6 cut(s)	252, 407, 1931, 2086, 2368, 2396
Hin1I	GRCGYC	1 cut(s)	2394
Hin6I	GCGC	6 cut(s)	250, 405, 1929, 2084, 2366, 2394
HinP1I	GCGC	6 cut(s)	250, 405, 1929, 2084, 2366, 2394
HindIII	AAGCTT	3 cut(s)	340, 1008, 2091
HpaII	CCGG	6 cut(s)	21, 329, 398, 1085, 2004, 2285
HphI	GGTGA	8 cut(s)	318, 577, 842, 1092, 1577, 1583, 2136, 2437
Hpy166II	GTNNAC	3 cut(s)	1588, 2020, 2267
Hpy188I	TCNGA	7 cut(s)	738, 843, 858, 1243, 1604, 1824, 2260
Hpy188III	TCNNGA	7 cut(s)	385, 746, 1364, 1595, 2594, 2619, 2660
Hpy8I	GTNNAC	3 cut(s)	1588, 2020, 2267
Hpy99I	CGWCG	1 cut(s)	1397
HpyCH4III	ACNGT	4 cut(s)	724, 810, 893, 2492
HpyF10VI	GCNNNNNNNGC	8 cut(s)	692, 771, 1034, 1168, 2090, 2114, 2384, 2393
HpyF3I	CTNAG	9 cut(s)	45, 71, 335, 434, 497, 1347, 1612, 2118, 2318
Hsp92I	GRCGYC	1 cut(s)	2394
HspAI	GCGC	6 cut(s)	250, 405, 1929, 2084, 2366, 2394
KasI	GGCGCC	1 cut(s)	2393
Kzo9I	GATC	8 cut(s)	295, 1042, 1165, 1494, 1597, 1741, 2232, 2323
LguI	GCTCTTC	2 cut(s)	51, 755
LweI	GCATC	3 cut(s)	682, 1661, 2591
MaeI	CTAG	7 cut(s)	108, 848, 915, 981, 1920, 2039, 2051
MaeIII	GTNAC	5 cut(s)	78, 460, 565, 1456, 2425
MalI	GATC	8 cut(s)	297, 1044, 1167, 1496, 1599, 1743, 2234, 2325
MboI	GATC	8 cut(s)	295, 1042, 1165, 1494, 1597, 1741, 2232, 2323
MfeI	CAATTG	2 cut(s)	1341, 1941
MflI	RGATCY	2 cut(s)	1494, 2323
MhlI	GDGCHC	3 cut(s)	63, 107, 1979
Mly113I	GGCGCC	1 cut(s)	2394
MlyI	GAGTC	5 cut(s)	86, 201, 742, 758, 1376
MmeI	TCCRAC	4 cut(s)	63, 503, 948, 2329
Mph1103I	ATGCAT	1 cut(s)	2439
MroXI	GAANNNNTTC	3 cut(s)	34, 1767, 2417
MseI	TTAA	4 cut(s)	804, 1356, 1953, 2330
MslI	CAYNNNNRTG	2 cut(s)	422, 2438
MspA1I	CMGCKG	2 cut(s)	8, 2317
MspCI	CTTAAG	1 cut(s)	2329
MspI	CCGG	6 cut(s)	21, 329, 398, 1085, 2004, 2285
MspR9I	CCNGG	4 cut(s)	53, 1085, 2005, 2249
MunI	CAATTG	2 cut(s)	1341, 1941
Mva1269I	GAATGC	2 cut(s)	429, 2485
MvaI	CCWGG	2 cut(s)	53, 2249
MvnI	CGCG	3 cut(s)	223, 405, 1635
MwoI	GCNNNNNNNGC	8 cut(s)	692, 771, 1034, 1168, 2090, 2114, 2384, 2393
NarI	GGCGCC	1 cut(s)	2394
NciI	CCSGG	2 cut(s)	1085, 2005
NcoI	CCATGG	5 cut(s)	245, 634, 1736, 1959, 2079
NdeII	GATC	8 cut(s)	295, 1042, 1165, 1494, 1597, 1741, 2232, 2323
NheI	GCTAGC	1 cut(s)	1919
NlaIV	GGNNCC	5 cut(s)	153, 611, 1089, 2325, 2395
NmeAIII	GCCGAG	2 cut(s)	213, 684
NmuCI	GTSAC	5 cut(s)	78, 460, 565, 1456, 2425
NsiI	ATGCAT	1 cut(s)	2439
NspI	RCATGY	2 cut(s)	2437, 2441
NspV	TTCGAA	1 cut(s)	588
PaeI	GCATGC	1 cut(s)	2437
PaeR7I	CTCGAG	2 cut(s)	1924, 2222
PceI	AGGCCT	1 cut(s)	474
PciSI	GCTCTTC	2 cut(s)	51, 755
PctI	GAATGC	2 cut(s)	429, 2485
PdmI	GAANNNNTTC	3 cut(s)	34, 1767, 2417
PfeI	GAWTC	8 cut(s)	742, 1337, 1516, 1559, 1660, 1819, 2047, 2418
PflMI	CCANNNNNTGG	1 cut(s)	2248
PfoI	TCCNGGA	1 cut(s)	51
PleI	GAGTC	5 cut(s)	85, 201, 741, 757, 1375
PluTI	GGCGCC	1 cut(s)	2397
PpsI	GAGTC	5 cut(s)	85, 201, 741, 757, 1375
Psp124BI	GAGCTC	1 cut(s)	63
Psp6I	CCWGG	2 cut(s)	51, 2247
PspGI	CCWGG	2 cut(s)	51, 2247
PspN4I	GGNNCC	5 cut(s)	153, 611, 1089, 2325, 2395
PspPI	GGNCC	1 cut(s)	2354
PspXI	VCTCGAGB	2 cut(s)	1924, 2222
PstI	CTGCAG	1 cut(s)	1120
PstNI	CAGNNNCTG	2 cut(s)	52, 433
PsuI	RGATCY	2 cut(s)	1494, 2323
PvuII	CAGCTG	2 cut(s)	8, 2317
RsaI	GTAC	3 cut(s)	112, 721, 2187
RsaNI	GTAC	3 cut(s)	111, 720, 2186
RseI	CAYNNNNRTG	2 cut(s)	422, 2438
SacI	GAGCTC	1 cut(s)	63
SapI	GCTCTTC	2 cut(s)	51, 755
SaqAI	TTAA	4 cut(s)	804, 1356, 1953, 2330
Sau3AI	GATC	8 cut(s)	295, 1042, 1165, 1494, 1597, 1741, 2232, 2323
Sau96I	GGNCC	1 cut(s)	2354
ScaI	AGTACT	3 cut(s)	112, 721, 2187
SchI	GAGTC	5 cut(s)	86, 201, 742, 758, 1376
ScrFI	CCNGG	4 cut(s)	53, 1085, 2005, 2249
SduI	GDGCHC	3 cut(s)	63, 107, 1979
SfaNI	GCATC	3 cut(s)	682, 1661, 2591
SfcI	CTRYAG	1 cut(s)	1116
SfoI	GGCGCC	1 cut(s)	2395
Sfr274I	CTCGAG	2 cut(s)	1924, 2222
SfuI	TTCGAA	1 cut(s)	588
SlaI	CTCGAG	2 cut(s)	1924, 2222
SmiMI	CAYNNNNRTG	2 cut(s)	422, 2438
SmlI	CTYRAG	6 cut(s)	1364, 1481, 1924, 2222, 2329, 2642
SmoI	CTYRAG	6 cut(s)	1364, 1481, 1924, 2222, 2329, 2642
SpeI	ACTAGT	1 cut(s)	107
SphI	GCATGC	1 cut(s)	2437
SseBI	AGGCCT	1 cut(s)	474
SsiI	CCGC	9 cut(s)	185, 221, 229, 256, 630, 828, 1633, 1856, 2345
SspDI	GGCGCC	1 cut(s)	2393
SspMI	CTAG	7 cut(s)	108, 848, 915, 981, 1920, 2039, 2051
SstI	GAGCTC	1 cut(s)	63
StuI	AGGCCT	1 cut(s)	474
StyD4I	CCNGG	4 cut(s)	51, 1083, 2003, 2247
StyI	CCWWGG	5 cut(s)	245, 634, 1736, 1959, 2079
TaaI	ACNGT	4 cut(s)	724, 810, 893, 2492
TatI	WGTACW	3 cut(s)	110, 719, 2185
TfiI	GAWTC	8 cut(s)	742, 1337, 1516, 1559, 1660, 1819, 2047, 2418
Tru1I	TTAA	4 cut(s)	804, 1356, 1953, 2330
Tru9I	TTAA	4 cut(s)	804, 1356, 1953, 2330
TscAI	CASTG	5 cut(s)	75, 469, 2304, 2497, 2525
TseFI	GTSAC	5 cut(s)	78, 460, 565, 1456, 2425
Tsp45I	GTSAC	5 cut(s)	78, 460, 565, 1456, 2425
TspGWI	ACGGA	1 cut(s)	552
TspRI	CASTG	5 cut(s)	75, 469, 2304, 2497, 2525
Van91I	CCANNNNNTGG	1 cut(s)	2248
Vha464I	CTTAAG	1 cut(s)	2329
XapI	RAATTY	7 cut(s)	12, 34, 780, 1202, 2100, 2167, 2669
XceI	RCATGY	2 cut(s)	2437, 2441
XhoI	CTCGAG	2 cut(s)	1924, 2222
XmnI	GAANNNNTTC	3 cut(s)	34, 1767, 2417
XspI	CTAG	7 cut(s)	108, 848, 915, 981, 1920, 2039, 2051
ZrmI	AGTACT	3 cut(s)	112, 721, 2187
Zsp2I	ATGCAT	1 cut(s)	2439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.