Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0014333

Physical Location & Seq

Genomic Coordinates Reverse (-)

60405024 .. 60408138

Gene Structure 3115 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG375000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2625 bp

ATGGCGGCAGCAGAGAATTCATCAGGTGGCGGAAGGAGGAATTCCAACACTCAGCTCCTGGAAGAGCTAGAAGCGCTCAGTGAGTCACTCTACCAATCCCACACGTCCACCACTACCACTCGAAGAACTGCCTCCCTTGCTCTTCCGCGAAGCTCGAATCCTGCAATCCCATCAAGAGATGAAATTGCTGCCGCGGCTAATAGCACCAAACCACTGAGAAGACGTTTGTCTTTGTCGCCTTGGCGCTCACGTCCAAACGAAAATGAACACAAAGATCGAGGCAAGGGGACGAGTAGTATTAATCAGCTGGAGCTGAAGAGCAATGTGGAGGAGAGATCAAGTAGCAAAGAGAAAAAGGGTATTTGGAATTGGAAGCCGATTCGTGCTATATCCCACATTGGAATGCACAAGATCAGCTGCTTGTTTTCGGTAGAGGTTGTCAGTGCACAAGGCCTTCCTGCTTCGATGAATGGCCTTCGTCTTTCGATTTGTGTTAGGAAGAAGGAGAGCAAAGACGGAGCAGTTCAAACAATGCCATCAAGGGTCACACAAGGAGCTGCAGATTTCGAAGAGACCCTTTTCTTTAGGTGCCATGTGTATTGTAGTTCTGGCCAGGGGAAGCCGATGAAGTTTGAGCCACGGCCCTTTTGGATATATGTGTTTGCAGTTGATGCTCAGGAGCTTGATTTTGGGAGAAATTCCGTGGATTTGAGTCAGCTGATTCAGGAGTCCATTGAGAAGAGCCATGAAGGTAAACGAATTCGCCAATGGGATAAGAGCTTTAGATTGTCAGGGAAGGCAAAAGGTGGGGAGCTTGTTCTGAAGTTGGGGTTTCAGATTATGGAGAAAGATGGAGGAGTTGGAATCTATAGTCAAGCTTCCAGGACATTCTCGTCTTCTTTTGCAAGGAAGCAGTCGAAGACTTCTTTTAGTGTGCCTAGTCCAAAGCTATCAAGCAGAGAAGCATCGACTCCTTCACAAATAGGACAATCTGCTCATGATCTTCATGGAATCGATGAGCTGAATCTTGATGAACCCAACCCAGTTCCAGTATCCTCTTCTTCTTCGGCGCAGAAGCCAAAAGAACCAGAAGCACTGAAGGTAGAGGATCTTGATCTCCCAGACTTCGAAGTAGTGGATAAGGGAGTTGAATTCCAAGACAAGGAAGGGGAATATGGGGAAGCACAATCAGAAATATCCCTTGATGAAAAATCAGCAATATCGAGTGAGGTTGTGAAGGAAATTGTGCAAGACCAAGTGCATATGAAAAGATTGACAGAGCTGGATTCGATTGCTCAGCAGATTAAAGCTCTTGAATCTATGATGGGGAAAGAAAAAACTGTCGCCAAAGACGAAGAAATTGGATCACAAATATTGGAGGCAGATGAAGAAACAGTTACCAAGGAGTTCCTTCAAATGCTTGAGGATGAGGATATCATAAATGAGTATAAGCTCACCCAAAGTGATATTCCACCATTACAGCTTGAAGGAGCTGAGGATTCCACAGAGGCTGAATCCGCAGTATTCCTTCCTGATCTAGGAAAGAGCTTAGGCTGTGTGGTTCAAACCAGAGATGGAGGTTACTTGGCAGCCATGAATCCTCTGGATACTGTGGTAGCAAGGAAAGATACACCGAAACTTGCCATGCAAATATCAAAACCATTTGTTTTGCCATGGGACCAGTCCATGAGTGGCTTCGAATTGTTCCAAAGGATTGCAGCCATTGGACTCGATGAATTGAATTCCCAAATTTCAACTTTGACGAGCATGGATGACCTGATGGATAAAACCGCAGAACAGATAGCTTTCGAAGGCATTGCTTCCGCAATCATCCAAGGGAGGAACAAAGAAGGTGCAAGCTCGAGTGCTGCACGAACCATTGCTGCTGTGAAAACCATGGCAACTGCAATGAGTGCCGGTAGAAAAGAGAGGATTTCCACAGGGATTTGGAATGTGAATGAAAACCCGTTAACAGCAGAGGAGATTCTTGCATTCTCAATGCAGAAAATTGAGTCCATGGCTCTTGAAGCTTTGAAGATTCAGGCTGAAATGGCAGAGGAAGAAGCCCCATTTGATGTTTCGCCATTGATTGGCACCACAGCAACTGGAGGAAAAGTTCAAAATCAACCACTTGCTTCTTCAATATCGCTCGAGGACTGGATCAAAGACCACAGCTTGGTTGGTGTAGATGACTTATTACAACCAGGTGGTGGTCATACCGAGACAATCACACTGGCAGTGGTTGTTCAGCTGAGGGATCCGGTAAGGCGGTATGAGGCTGTAGGAGGCCCAATGATTGCAGTTATCTATGCAACAAGAGCTGATAACACCGGTGCAGTGGATAAGAATGAGGAGGAGGAGAAGAGATTCAAAGTGGCGAGTTTGCATGTTGGAGGGTTAAAGGTGAGGTCAAAAGGCGTTAGGAGAAATGCATGGGACAGTGAGAAGCAGAGACTAACTGCAATGCAGTGGCTGGTGGCTTATGGATTTGCAAAGGCAGGGAAGAAAGGCAAACATAGTGTGTCATCGAAAGGTCAAGATCTGTTATGGAGCATTTCATCAAGAGTAATGGCTGATATGTGGCTCAAATACATGAGAAACCCAGATGTAAAGTTTACCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006810 GO:0006928 GO:0006950 GO:0006970 GO:0006996 GO:0007097 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009657 GO:0009658 GO:0009787 GO:0009902 GO:0009903 GO:0009966 GO:0009987 GO:0010029 GO:0010646 GO:0016020 GO:0016043 GO:0019750 GO:0023051 GO:0030029 GO:0030048 GO:0030705 GO:0031022 GO:0042592 GO:0044424 GO:0044444 GO:0044464 GO:0046907 GO:0048580 GO:0048583 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051640 GO:0051641 GO:0051644 GO:0051647 GO:0051649 GO:0051656 GO:0051667 GO:0055081 GO:0055088 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0099515 GO:1900140 GO:1901419 GO:1902265 GO:1905957 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NT-C2

Protein Analysis

874

Amino Acids

96.07

Weight (kDa)

5.58

Isoelectric Point (pI)

46.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NT-C2	PF10358	136 - 284	1.5e-14	N-terminal C2 in EEIG1 and EHBP1 proteins
PMI1_PMIR1-2_C	PF21745	546 - 694	5.9e-59	PMI1/PMIR1-2, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014333)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42550
fragaria_vesca	FvH4_2g26870
malus_domestica	MD08G1162900.v1.1
prunus_persica	Prupe.1G498000_v2.0.a1
pyrus_communis	pycom08g14070 pycom15g31260
rosa_chinensis	RchiOBHm_Chr6g0295721
rosa_laevigata	RLG00000011772
rosa_multiflora	Rmu_sc0007372.1_g000001 Rmu_sc0007986.1_g000004
rosa_roxburghii	Rroxscaffold_7G00172180
rosa_rugosa	Rorug06G0253800
rosa_samantha	Rh6AG367200 Rh6BG375000 Rh6CG381300 Rh6DG368100
rosa_wichuraiana	Rw6G032050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	892
AccB1I	GGYRCC	2 cut(s)	588, 2093
AccB7I	CCANNNNNTGG	3 cut(s)	2090, 2176, 2210
AccII	CGCG	2 cut(s)	148, 194
AciI	CCGC	9 cut(s)	5, 30, 146, 192, 194, 1518, 1791, 1824, 2269
AclWI	GGATC	5 cut(s)	1116, 1372, 2168, 2252, 2265
AcoI	YGGCCR	1 cut(s)	610
AcsI	RAATTY	7 cut(s)	16, 40, 697, 759, 1151, 1741, 1749
AcuI	CTGAAG	3 cut(s)	335, 842, 1118
AfeI	AGCGCT	1 cut(s)	75
AfiI	CCNNNNNNNGG	5 cut(s)	1162, 1538, 2090, 2176, 2210
AflIII	ACRYGT	1 cut(s)	102
AgeI	ACCGGT	1 cut(s)	2330
AjiI	CACGTC	2 cut(s)	105, 251
AjnI	CCWGG	4 cut(s)	57, 612, 881, 2203
AjuI	GAANNNNNNNTTGG	2 cut(s)	1452, 1484
AloI	GAACNNNNNNTCC	2 cut(s)	2100, 2132
Alw21I	GWGCWC	1 cut(s)	448
Alw26I	GTCTC	3 cut(s)	566, 2216, 2446
Alw44I	GTGCAC	1 cut(s)	444
AlwI	GGATC	5 cut(s)	1116, 1372, 2168, 2252, 2265
AlwNI	CAGNNNCTG	4 cut(s)	58, 1511, 2105, 2473
Ama87I	CYCGRG	2 cut(s)	1861, 2150
Aor51HI	AGCGCT	1 cut(s)	75
AoxI	GGCC	5 cut(s)	451, 472, 610, 641, 2287
ApaLI	GTGCAC	1 cut(s)	444
ApeKI	GCWGC	8 cut(s)	8, 188, 417, 557, 1589, 1718, 1868, 1883
ApoI	RAATTY	7 cut(s)	16, 40, 697, 759, 1151, 1741, 1749
AseI	ATTAAT	1 cut(s)	300
AsiGI	ACCGGT	1 cut(s)	2330
Asp700I	GAANNNNTTC	1 cut(s)	2366
AspLEI	GCGC	3 cut(s)	76, 246, 1072
AspS9I	GGNCC	3 cut(s)	642, 1678, 2288
AsuHPI	GGTGA	2 cut(s)	1447, 2416
AsuII	TTCGAA	4 cut(s)	567, 1128, 1698, 1809
AvaI	CYCGRG	2 cut(s)	1861, 2150
AvaII	GGWCC	1 cut(s)	1678
BaeGI	GKGCMC	1 cut(s)	448
BaeI	ACNNNNGTAYC	2 cut(s)	1599, 1632
BalI	TGGCCA	1 cut(s)	612
BamHI	GGATCC	1 cut(s)	2257
BanI	GGYRCC	2 cut(s)	588, 2093
BbsI	GAAGAC	3 cut(s)	226, 888, 926
Bbv12I	GWGCWC	1 cut(s)	448
BbvCI	CCTCAGC	2 cut(s)	1494, 2252
BbvI	GCAGC	8 cut(s)	20, 175, 404, 544, 1601, 1730, 1855, 1870
BccI	CCATC	6 cut(s)	178, 544, 845, 1318, 1568, 1774
BceAI	ACGGC	1 cut(s)	656
BcgI	CGANNNNNNTGC	2 cut(s)	1799, 1833
BciT130I	CCWGG	4 cut(s)	59, 614, 883, 2205
BciVI	GTATCC	2 cut(s)	1063, 1600
BcoDI	GTCTC	3 cut(s)	566, 2216, 2446
BfaI	CTAG	3 cut(s)	68, 939, 1538
BfmI	CTRYAG	3 cut(s)	558, 868, 2280
BfoI	RGCGCY	2 cut(s)	77, 247
BfuI	GTATCC	2 cut(s)	1063, 1600
BglII	AGATCT	1 cut(s)	2539
BlpI	GCTNAGC	1 cut(s)	1296
Bme1390I	CCNGG	4 cut(s)	59, 614, 883, 2205
Bme18I	GGWCC	1 cut(s)	1678
BmeT110I	CYCGRG	2 cut(s)	1861, 2150
BmgBI	CACGTC	2 cut(s)	105, 251
BmgT120I	GGNCC	3 cut(s)	642, 1678, 2288
BmiI	GGNNCC	4 cut(s)	590, 1679, 2095, 2259
BmrFI	CCNGG	4 cut(s)	59, 614, 883, 2205
BmrI	ACTGGG	1 cut(s)	1037
BmsI	GCATC	2 cut(s)	661, 974
BmuI	ACTGGG	1 cut(s)	1037
BoxI	GACNNNNGTC	1 cut(s)	226
BpiI	GAAGAC	3 cut(s)	226, 888, 926
BpmI	CTGGAG	2 cut(s)	329, 2127
Bpu10I	CCTNAGC	4 cut(s)	675, 1494, 1549, 2252
Bpu1102I	GCTNAGC	1 cut(s)	1296
Bpu14I	TTCGAA	4 cut(s)	567, 1128, 1698, 1809
BpuEI	CTTGAG	1 cut(s)	1442
Bsa29I	ATCGAT	1 cut(s)	1014
BsaBI	GATNNNNATC	1 cut(s)	1113
BsaI	GGTCTC	1 cut(s)	566
BsaWI	WCCGGW	2 cut(s)	2260, 2330
BsaXI	ACNNNNNCTCC	2 cut(s)	2100, 2130
Bsc4I	CCNNNNNNNGG	5 cut(s)	1162, 1538, 2090, 2176, 2210
Bse118I	RCCGGY	2 cut(s)	1916, 2330
Bse1I	ACTGG	6 cut(s)	1043, 1049, 1681, 2110, 2162, 2238
Bse3DI	GCAATG	5 cut(s)	328, 1815, 1878, 1914, 2469
Bse8I	GATNNNNATC	1 cut(s)	1113
BseBI	CCWGG	4 cut(s)	59, 614, 883, 2205
BseCI	ATCGAT	1 cut(s)	1014
BseGI	GGATG	3 cut(s)	1432, 1777, 1830
BseJI	GATNNNNATC	1 cut(s)	1113
BseLI	CCNNNNNNNGG	5 cut(s)	1162, 1538, 2090, 2176, 2210
BseMI	GCAATG	5 cut(s)	328, 1815, 1878, 1914, 2469
BseMII	CTCAG	7 cut(s)	65, 91, 206, 689, 1310, 1485, 2243
BseNI	ACTGG	6 cut(s)	1043, 1049, 1681, 2110, 2162, 2238
BseRI	GAGGAG	6 cut(s)	344, 870, 1994, 2366, 2369, 2372
BseSI	GKGCMC	1 cut(s)	448
BseXI	GCAGC	8 cut(s)	20, 175, 404, 544, 1601, 1730, 1855, 1870
BsgI	GTGCAG	2 cut(s)	1854, 2355
Bsh1236I	CGCG	2 cut(s)	148, 194
BshFI	GGCC	5 cut(s)	453, 474, 612, 643, 2289
BshNI	GGYRCC	2 cut(s)	588, 2093
BshTI	ACCGGT	1 cut(s)	2330
BshVI	ATCGAT	1 cut(s)	1014
BsiHKAI	GWGCWC	1 cut(s)	448
BsiHKCI	CYCGRG	2 cut(s)	1861, 2150
BsiSI	CCGG	3 cut(s)	1917, 2261, 2331
BslFI	GGGAC	3 cut(s)	301, 1691, 2450
BslI	CCNNNNNNNGG	5 cut(s)	1162, 1538, 2090, 2176, 2210
BsmAI	GTCTC	3 cut(s)	566, 2216, 2446
BsmFI	GGGAC	3 cut(s)	301, 1691, 2450
BsmI	GAATGC	2 cut(s)	408, 1991
BsnI	GGCC	5 cut(s)	453, 474, 612, 643, 2289
Bso31I	GGTCTC	1 cut(s)	566
BsoBI	CYCGRG	2 cut(s)	1861, 2150
Bsp119I	TTCGAA	4 cut(s)	567, 1128, 1698, 1809
Bsp1286I	GDGCHC	1 cut(s)	448
Bsp1720I	GCTNAGC	1 cut(s)	1296
Bsp19I	CCATGG	3 cut(s)	1673, 1896, 2016
BspACI	CCGC	9 cut(s)	5, 30, 146, 192, 194, 1518, 1791, 1824, 2269
BspANI	GGCC	5 cut(s)	453, 474, 612, 643, 2289
BspCNI	CTCAG	7 cut(s)	64, 90, 207, 688, 1309, 1486, 2244
BspDI	ATCGAT	1 cut(s)	1014
BspFNI	CGCG	2 cut(s)	148, 194
BspHI	TCATGA	1 cut(s)	997
BspLI	GGNNCC	4 cut(s)	590, 1679, 2095, 2259
BspMAI	CTGCAG	1 cut(s)	562
BspPI	GGATC	5 cut(s)	1116, 1372, 2168, 2252, 2265
BspQI	GCTCTTC	4 cut(s)	57, 147, 311, 734
BspT104I	TTCGAA	4 cut(s)	567, 1128, 1698, 1809
BspT107I	GGYRCC	2 cut(s)	588, 2093
BspTNI	GGTCTC	1 cut(s)	566
BsrDI	GCAATG	5 cut(s)	328, 1815, 1878, 1914, 2469
BsrFI	RCCGGY	2 cut(s)	1916, 2330
BsrI	ACTGG	6 cut(s)	1043, 1049, 1681, 2110, 2162, 2238
BssAI	RCCGGY	2 cut(s)	1916, 2330
BssT1I	CCWWGG	6 cut(s)	239, 1401, 1673, 1834, 1896, 2016
Bst2UI	CCWGG	4 cut(s)	59, 614, 883, 2205
Bst4CI	ACNGT	4 cut(s)	1342, 1396, 1612, 2441
Bst6I	CTCTTC	7 cut(s)	57, 147, 311, 564, 734, 1063, 2357
BstAPI	GCANNNNNTGC	2 cut(s)	671, 1913
BstBI	TTCGAA	4 cut(s)	567, 1128, 1698, 1809
BstC8I	GCNNGC	1 cut(s)	1858
BstDEI	CTNAG	8 cut(s)	51, 77, 215, 675, 1296, 1494, 1549, 2252
BstDSI	CCRYGG	6 cut(s)	192, 638, 702, 1673, 1896, 2016
BstENI	CCTNNNNNAGG	1 cut(s)	1536
BstF5I	GGATG	3 cut(s)	1432, 1777, 1830
BstFNI	CGCG	2 cut(s)	148, 194
BstH2I	RGCGCY	2 cut(s)	77, 247
BstHHI	GCGC	3 cut(s)	76, 246, 1072
BstMAI	GTCTC	3 cut(s)	566, 2216, 2446
BstMWI	GCNNNNNNNGC	9 cut(s)	73, 137, 194, 671, 1517, 1913, 2027, 2051, 2318
BstNI	CCWGG	4 cut(s)	59, 614, 883, 2205
BstNSI	RCATGY	1 cut(s)	2390
BstPAI	GACNNNNGTC	1 cut(s)	226
BstSCI	CCNGG	4 cut(s)	57, 612, 881, 2203
BstSFI	CTRYAG	3 cut(s)	558, 868, 2280
BstSLI	GKGCMC	1 cut(s)	448
BstUI	CGCG	2 cut(s)	148, 194
BstV1I	GCAGC	8 cut(s)	20, 175, 404, 544, 1601, 1730, 1855, 1870
BstV2I	GAAGAC	3 cut(s)	226, 888, 926
BstX2I	RGATCY	3 cut(s)	1108, 2257, 2539
BstYI	RGATCY	3 cut(s)	1108, 2257, 2539
Bsu15I	ATCGAT	1 cut(s)	1014
BsuI	GTATCC	2 cut(s)	1063, 1600
BsuRI	GGCC	5 cut(s)	453, 474, 612, 643, 2289
BsuTUI	ATCGAT	1 cut(s)	1014
BtgI	CCRYGG	6 cut(s)	192, 638, 702, 1673, 1896, 2016
BtrI	CACGTC	2 cut(s)	105, 251
BtsCI	GGATG	3 cut(s)	1432, 1777, 1830
BtsI	GCAGTG	3 cut(s)	2244, 2343, 2474
BtsIMutI	CAGTG	9 cut(s)	85, 212, 448, 1094, 2231, 2244, 2343, 2446, 2474
Cac8I	GCNNGC	1 cut(s)	1858
CaiI	CAGNNNCTG	4 cut(s)	58, 1511, 2105, 2473
CciI	TCATGA	1 cut(s)	997
CfoI	GCGC	3 cut(s)	76, 246, 1072
Cfr10I	RCCGGY	2 cut(s)	1916, 2330
Cfr13I	GGNCC	3 cut(s)	642, 1678, 2288
Cfr42I	CCGCGG	1 cut(s)	195
ClaI	ATCGAT	1 cut(s)	1014
CsiI	ACCWGGT	1 cut(s)	2203
CspAI	ACCGGT	1 cut(s)	2330
DdeI	CTNAG	8 cut(s)	51, 77, 215, 675, 1296, 1494, 1549, 2252
DrdI	GACNNNNNNGTC	1 cut(s)	892
DseDI	GACNNNNNNGTC	1 cut(s)	892
EaeI	YGGCCR	1 cut(s)	610
Eam1104I	CTCTTC	7 cut(s)	57, 147, 311, 564, 734, 1063, 2357
EarI	CTCTTC	7 cut(s)	57, 147, 311, 564, 734, 1063, 2357
EciI	GGCGGA	1 cut(s)	45
Eco130I	CCWWGG	6 cut(s)	239, 1401, 1673, 1834, 1896, 2016
Eco147I	AGGCCT	1 cut(s)	453
Eco31I	GGTCTC	1 cut(s)	566
Eco32I	GATATC	1 cut(s)	1435
Eco47I	GGWCC	1 cut(s)	1678
Eco47III	AGCGCT	1 cut(s)	75
Eco57I	CTGAAG	3 cut(s)	335, 842, 1118
Eco88I	CYCGRG	2 cut(s)	1861, 2150
EcoNI	CCTNNNNNAGG	1 cut(s)	1536
EcoRI	GAATTC	5 cut(s)	16, 40, 759, 1151, 1741
EcoRII	CCWGG	4 cut(s)	57, 612, 881, 2203
EcoRV	GATATC	1 cut(s)	1435
EcoT14I	CCWWGG	6 cut(s)	239, 1401, 1673, 1834, 1896, 2016
EcoT22I	ATGCAT	1 cut(s)	2434
ErhI	CCWWGG	6 cut(s)	239, 1401, 1673, 1834, 1896, 2016
FalI	AAGNNNNNCTT	2 cut(s)	561, 593
FaqI	GGGAC	3 cut(s)	301, 1691, 2450
FauNDI	CATATG	1 cut(s)	1263
FokI	GGATG	3 cut(s)	1439, 1784, 1817
FspBI	CTAG	3 cut(s)	68, 939, 1538
GlaI	GCGC	3 cut(s)	75, 245, 1071
GsuI	CTGGAG	2 cut(s)	329, 2127
HaeII	RGCGCY	2 cut(s)	77, 247
HaeIII	GGCC	5 cut(s)	453, 474, 612, 643, 2289
HapII	CCGG	3 cut(s)	1917, 2261, 2331
HhaI	GCGC	3 cut(s)	76, 246, 1072
Hin6I	GCGC	3 cut(s)	74, 244, 1070
HinP1I	GCGC	3 cut(s)	74, 244, 1070
HincII	GTYRAC	1 cut(s)	1971
HindII	GTYRAC	1 cut(s)	1971
HindIII	AAGCTT	2 cut(s)	876, 2028
HpaI	GTTAAC	1 cut(s)	1971
HpaII	CCGG	3 cut(s)	1917, 2261, 2331
HphI	GGTGA	2 cut(s)	1447, 2416
Hpy166II	GTNNAC	5 cut(s)	108, 446, 755, 1971, 2616
Hpy188I	TCNGA	3 cut(s)	822, 837, 1192
Hpy8I	GTNNAC	5 cut(s)	108, 446, 755, 1971, 2616
HpyCH4III	ACNGT	4 cut(s)	1342, 1396, 1612, 2441
HpyCH4IV	ACGT	3 cut(s)	104, 223, 250
HpyF10VI	GCNNNNNNNGC	9 cut(s)	73, 137, 194, 671, 1517, 1913, 2027, 2051, 2318
HpyF3I	CTNAG	8 cut(s)	51, 77, 215, 675, 1296, 1494, 1549, 2252
HpySE526I	ACGT	3 cut(s)	104, 223, 250
HspAI	GCGC	3 cut(s)	74, 244, 1070
KspAI	GTTAAC	1 cut(s)	1971
KspI	CCGCGG	1 cut(s)	195
LguI	GCTCTTC	4 cut(s)	57, 147, 311, 734
LmnI	GCTCC	8 cut(s)	60, 310, 518, 554, 679, 811, 1490, 2550
Lsp1109I	GCAGC	8 cut(s)	20, 175, 404, 544, 1601, 1730, 1855, 1870
LweI	GCATC	2 cut(s)	661, 974
MabI	ACCWGGT	1 cut(s)	2203
MaeI	CTAG	3 cut(s)	68, 939, 1538
MaeII	ACGT	3 cut(s)	104, 223, 250
MaeIII	GTNAC	4 cut(s)	84, 544, 1396, 1580
MfeI	CAATTG	1 cut(s)	2620
MflI	RGATCY	3 cut(s)	1108, 2257, 2539
MhlI	GDGCHC	1 cut(s)	448
MlsI	TGGCCA	1 cut(s)	612
MluNI	TGGCCA	1 cut(s)	612
MlyI	GAGTC	6 cut(s)	92, 721, 737, 964, 1722, 2021
MmeI	TCCRAC	3 cut(s)	69, 841, 2371
Mox20I	TGGCCA	1 cut(s)	612
Mph1103I	ATGCAT	1 cut(s)	2434
MroXI	GAANNNNTTC	1 cut(s)	2366
MscI	TGGCCA	1 cut(s)	612
MseI	TTAA	4 cut(s)	300, 1305, 1970, 2399
MslI	CAYNNNNRTG	1 cut(s)	401
Msp20I	TGGCCA	1 cut(s)	612
MspA1I	CMGCKG	5 cut(s)	194, 307, 417, 718, 2251
MspI	CCGG	3 cut(s)	1917, 2261, 2331
MspR9I	CCNGG	4 cut(s)	59, 614, 883, 2205
MunI	CAATTG	1 cut(s)	2620
Mva1269I	GAATGC	2 cut(s)	408, 1991
MvaI	CCWGG	4 cut(s)	59, 614, 883, 2205
MvnI	CGCG	2 cut(s)	148, 194
MwoI	GCNNNNNNNGC	9 cut(s)	73, 137, 194, 671, 1517, 1913, 2027, 2051, 2318
NcoI	CCATGG	3 cut(s)	1673, 1896, 2016
NdeI	CATATG	1 cut(s)	1263
NlaIV	GGNNCC	4 cut(s)	590, 1679, 2095, 2259
NmuCI	GTSAC	2 cut(s)	84, 544
NsiI	ATGCAT	1 cut(s)	2434
NspI	RCATGY	1 cut(s)	2390
NspV	TTCGAA	4 cut(s)	567, 1128, 1698, 1809
PaeR7I	CTCGAG	2 cut(s)	1861, 2150
PagI	TCATGA	1 cut(s)	997
PceI	AGGCCT	1 cut(s)	453
PciSI	GCTCTTC	4 cut(s)	57, 147, 311, 734
PcsI	WCGNNNNNNNCGW	1 cut(s)	1350
PctI	GAATGC	2 cut(s)	408, 1991
PdmI	GAANNNNTTC	1 cut(s)	2366
PflMI	CCANNNNNTGG	3 cut(s)	2090, 2176, 2210
PfoI	TCCNGGA	2 cut(s)	57, 881
PinAI	ACCGGT	1 cut(s)	2330
PleI	GAGTC	6 cut(s)	91, 720, 736, 964, 1722, 2020
PpsI	GAGTC	6 cut(s)	91, 720, 736, 964, 1722, 2020
PshAI	GACNNNNGTC	1 cut(s)	226
PshBI	ATTAAT	1 cut(s)	300
Psp6I	CCWGG	4 cut(s)	57, 612, 881, 2203
PspGI	CCWGG	4 cut(s)	57, 612, 881, 2203
PspN4I	GGNNCC	4 cut(s)	590, 1679, 2095, 2259
PspPI	GGNCC	3 cut(s)	642, 1678, 2288
PspXI	VCTCGAGB	2 cut(s)	1861, 2150
PstI	CTGCAG	1 cut(s)	562
PstNI	CAGNNNCTG	4 cut(s)	58, 1511, 2105, 2473
PsuI	RGATCY	3 cut(s)	1108, 2257, 2539
PvuII	CAGCTG	4 cut(s)	307, 417, 718, 2251
RseI	CAYNNNNRTG	1 cut(s)	401
SacII	CCGCGG	1 cut(s)	195
SapI	GCTCTTC	4 cut(s)	57, 147, 311, 734
SaqAI	TTAA	4 cut(s)	300, 1305, 1970, 2399
Sau96I	GGNCC	3 cut(s)	642, 1678, 2288
SchI	GAGTC	6 cut(s)	92, 721, 737, 964, 1722, 2021
ScrFI	CCNGG	4 cut(s)	59, 614, 883, 2205
SduI	GDGCHC	1 cut(s)	448
SexAI	ACCWGGT	1 cut(s)	2203
SfaNI	GCATC	2 cut(s)	661, 974
SfcI	CTRYAG	3 cut(s)	558, 868, 2280
Sfr274I	CTCGAG	2 cut(s)	1861, 2150
Sfr303I	CCGCGG	1 cut(s)	195
SfuI	TTCGAA	4 cut(s)	567, 1128, 1698, 1809
SgrAI	CRCCGGYG	1 cut(s)	2330
SgrBI	CCGCGG	1 cut(s)	195
SinI	GGWCC	1 cut(s)	1678
SlaI	CTCGAG	2 cut(s)	1861, 2150
SmiMI	CAYNNNNRTG	1 cut(s)	401
SmlI	CTYRAG	3 cut(s)	1421, 1861, 2150
SmoI	CTYRAG	3 cut(s)	1421, 1861, 2150
SseBI	AGGCCT	1 cut(s)	453
SsiI	CCGC	9 cut(s)	5, 30, 146, 192, 194, 1518, 1791, 1824, 2269
SspI	AATATT	1 cut(s)	1374
SspMI	CTAG	3 cut(s)	68, 939, 1538
StuI	AGGCCT	1 cut(s)	453
StyD4I	CCNGG	4 cut(s)	57, 612, 881, 2203
StyI	CCWWGG	6 cut(s)	239, 1401, 1673, 1834, 1896, 2016
TaaI	ACNGT	4 cut(s)	1342, 1396, 1612, 2441
TaiI	ACGT	3 cut(s)	107, 226, 253
TauI	GCSGC	3 cut(s)	8, 194, 197
Tru1I	TTAA	4 cut(s)	300, 1305, 1970, 2399
Tru9I	TTAA	4 cut(s)	300, 1305, 1970, 2399
TscAI	CASTG	9 cut(s)	85, 219, 448, 1101, 2238, 2244, 2343, 2446, 2474
TseFI	GTSAC	2 cut(s)	84, 544
TseI	GCWGC	8 cut(s)	8, 188, 417, 557, 1589, 1718, 1868, 1883
Tsp45I	GTSAC	2 cut(s)	84, 544
TspGWI	ACGGA	2 cut(s)	531, 691
TspRI	CASTG	9 cut(s)	85, 219, 448, 1101, 2238, 2244, 2343, 2446, 2474
Van91I	CCANNNNNTGG	3 cut(s)	2090, 2176, 2210
VneI	GTGCAC	1 cut(s)	444
VpaK11BI	GGWCC	1 cut(s)	1678
VspI	ATTAAT	1 cut(s)	300
XagI	CCTNNNNNAGG	1 cut(s)	1536
XapI	RAATTY	7 cut(s)	16, 40, 697, 759, 1151, 1741, 1749
XceI	RCATGY	1 cut(s)	2390
XcmI	CCANNNNNNNNNTGG	4 cut(s)	645, 1600, 1688, 2177
XhoI	CTCGAG	2 cut(s)	1861, 2150
XmnI	GAANNNNTTC	1 cut(s)	2366
XspI	CTAG	3 cut(s)	68, 939, 1538
Zsp2I	ATGCAT	1 cut(s)	2434

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.