Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0016352

Physical Location & Seq

Genomic Coordinates Forward (+)

27651402 .. 27652910

Gene Structure 1509 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1213200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGAAAATGTTCAGGCGTTTCTCCAACTCTCACAATCTCGACTCTTTCTTATCACAATCCACACCCCCATTTCATTCCCCCATTGTTCCTCCCAAAACCCTAGTCTCACACGAGCCCTCTCCTCCTGGCAAATTCTCCAATTCAAATCTGCCCCACACAGAAGCTGCTTTCTCCTCTCTGAGTTCCTTGATTTCCTTATTTGGGTCAAGCCCATCTTCTCAAAATTCCCAATCTTTGCTGACTATTTACTCACTCAAGCATCAATGTCACGGACGCGTTCAGCCATTTTCCACTGCGCCTTCCGATGTGTTCGAATCTTCCTCGCCGCCAAGAGCTTCGGGTTCCGATGAGGGTAATCGATTTGGGAATGTGGTTATTGATGGGATTCGACCGGATGAGACTTCAAATCAAGTTTTGGAAATTATTGATATGATCAGAATGGGTGAGAGTGATTTAAAGTCCAAGTTGAATTCCATGAATGTAAGTCTATCTCCTGCCTCCATTACTCGGGTTTTCGAGGTTTTGAATTCTGAAAAGGTACCTGCTTTATGTTTCTTTGATTGGATTAGGGATTCACAACCCATTGGTTGTTATGGGAATGAAATATGTAGCTTGGTTATTGATAATTGTGGGCGGGTAGGTGATTACCCTGCAATGCTTCAGATTTTGAAAGATTTGCAATCCGAGGAAATTCGTCTCACCCAAAAGGCATTTGGGTTTATATCTCCAAATAAGGCTTCTGTTGTCAAAGTGGTAGAGGTTTTGAATAAAGTTGGTGGACTGTGTCGGGCAACTGGAGTGCTCTCTTTGATTGAGATGTTTAGTGTCAAGGGTTTATTTGAAATGGCTGAGTTTGTGATGAAGAAAACAGAGAGGAAGAGATGTTATTACATTATAATGATCAGAGAAACTTGTCGGGGAAGAAATTTTGGCAGAGCTGTTGATATGCTTGATGAGATGAGGCAAGTGGGTTGTGACCCAGATGCCAAAACTTACAATTATCTACTCAGTAGCTTATGCAAGAACCATAAATTTGCTGAAGCTACTAAACTATATGAAGAAATGCTAGAAAGGAATTGTCAACCTGATGGCATAACCTACGAAATATTTATTTTTTACTTATTTAAAGTTGGAAATCTTGATTTTGCAAGACAGTTATTTGATAGAATGATATTGGAGGGCATTAAACCTCGTGTTTCAACACATGCTGCCTTTATCAAGGGTTATTTTAACTTGCGGAGATATAAGGAAGCATATAATTACGTTGTTGATTTAGCTGCTAAAGACAGTTTCTCTAGCAGTTCAGTGTACAGCTTGCTGGCAAGGCTTTATATGAAGGAAGGAAATGCAGTCGTTGCCCAAAATATTCTGACCGAAATGATTAATAAGGGTTTTAGACCAGATTATTCAGTGTATGTGAGAGTTTTGAAACGGCTGTCCAAGTCAGGCAGAAGTGGTTTGGCTGAAGATTTGGAAAGCAGGTTCTCTAGTCTAGCAGGAACTGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2

Protein Analysis

503

Amino Acids

56.3

Weight (kDa)

8.71

Isoelectric Point (pI)

46.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	297 - 341	8e-10	PPR repeat family
PPR_1	PF12854	324 - 355	6.2e-08	PPR repeat
PPR_2	PF13041	331 - 371	2.5e-12	PPR repeat family
PPR	PF01535	331 - 360	2.6e-06	PPR repeat
PPR_3	PF13812	351 - 409	1.6e-06	Pentatricopeptide repeat domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016352)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g33340
malus_domestica	MD08G1213200.v1.1
prunus_persica	Prupe.1G544500_v2.0.a1
pyrus_communis	pycom08g18310
rosa_chinensis	RchiOBHm_Chr7g0232061
rosa_laevigata	RLG00000001396
rosa_multiflora	Rmu_sc0004618.1_g000017 Rmu_sc0009837.1_g000001
rosa_roxburghii	Rroxscaffold_3G00228850
rosa_rugosa	Rorug07G0268800
rosa_samantha	Rh7AG423200 Rh7BG397400 Rh7CG443600 Rh7DG415500
rosa_wichuraiana	Rw7G035010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	896
Acc36I	ACCTGC	2 cut(s)	550, 1470
Acc65I	GGTACC	1 cut(s)	538
AccB1I	GGYRCC	1 cut(s)	538
AccII	CGCG	1 cut(s)	276
AciI	CCGC	3 cut(s)	326, 634, 1237
AcsI	RAATTY	7 cut(s)	131, 223, 469, 526, 690, 925, 1031
AcuI	CTGAAG	3 cut(s)	644, 1059, 1485
AfaI	GTAC	2 cut(s)	540, 1310
AfiI	CCNNNNNNNGG	2 cut(s)	201, 507
AflIII	ACRYGT	1 cut(s)	274
AgsI	TTSAA	9 cut(s)	144, 405, 469, 526, 670, 766, 842, 1200, 1429
AjnI	CCWGG	1 cut(s)	124
AjuI	GAANNNNNNNTTGG	2 cut(s)	721, 753
AloI	GAACNNNNNNTCC	2 cut(s)	1466, 1498
AluBI	AGCT	8 cut(s)	164, 335, 612, 938, 1014, 1043, 1277, 1314
AluI	AGCT	8 cut(s)	164, 335, 612, 938, 1014, 1043, 1277, 1314
Alw21I	GWGCWC	1 cut(s)	804
Alw26I	GTCTC	3 cut(s)	109, 392, 701
AlwNI	CAGNNNCTG	2 cut(s)	164, 1502
Ama87I	CYCGRG	1 cut(s)	507
ApeKI	GCWGC	3 cut(s)	164, 1208, 1277
ApoI	RAATTY	7 cut(s)	131, 223, 469, 526, 690, 925, 1031
ArsI	GACNNNNNNTTYG	2 cut(s)	87, 119
AseI	ATTAAT	1 cut(s)	1383
Asp700I	GAANNNNTTC	1 cut(s)	8
Asp718I	GGTACC	1 cut(s)	538
AspLEI	GCGC	1 cut(s)	298
AsuHPI	GGTGA	3 cut(s)	455, 653, 691
AsuII	TTCGAA	1 cut(s)	312
AvaI	CYCGRG	1 cut(s)	507
BanI	GGYRCC	1 cut(s)	538
BanII	GRGCYC	1 cut(s)	117
BauI	CACGAG	2 cut(s)	110, 1191
Bbv12I	GWGCWC	1 cut(s)	804
BbvI	GCAGC	3 cut(s)	151, 1195, 1264
BccI	CCATC	3 cut(s)	220, 374, 1082
BceAI	ACGGC	1 cut(s)	1448
BciT130I	CCWGG	1 cut(s)	126
BclI	TGATCA	2 cut(s)	432, 900
BcoDI	GTCTC	3 cut(s)	109, 392, 701
BfaI	CTAG	5 cut(s)	101, 1067, 1296, 1488, 1493
BfuAI	ACCTGC	2 cut(s)	550, 1470
BisI	GCNGC	4 cut(s)	165, 326, 1209, 1278
BlsI	GCNGC	4 cut(s)	166, 327, 1210, 1279
Bme1390I	CCNGG	1 cut(s)	126
BmeT110I	CYCGRG	1 cut(s)	507
BmiI	GGNNCC	2 cut(s)	343, 540
BmrFI	CCNGG	1 cut(s)	126
BmsI	GCATC	2 cut(s)	268, 973
BpmI	CTGGAG	1 cut(s)	816
Bpu14I	TTCGAA	1 cut(s)	312
BpuEI	CTTGAG	1 cut(s)	239
Bsa29I	ATCGAT	1 cut(s)	358
BsaJI	CCNNGG	1 cut(s)	684
BsaWI	WCCGGW	1 cut(s)	391
Bsc4I	CCNNNNNNNGG	2 cut(s)	201, 507
Bse1I	ACTGG	2 cut(s)	799, 1507
Bse3DI	GCAATG	1 cut(s)	660
BseBI	CCWGG	1 cut(s)	126
BseCI	ATCGAT	1 cut(s)	358
BseDI	CCNNGG	1 cut(s)	684
BseGI	GGATG	1 cut(s)	400
BseLI	CCNNNNNNNGG	2 cut(s)	201, 507
BseMI	GCAATG	1 cut(s)	660
BseMII	CTCAG	3 cut(s)	170, 840, 1021
BseNI	ACTGG	2 cut(s)	799, 1507
BseRI	GAGGAG	2 cut(s)	111, 163
BseXI	GCAGC	3 cut(s)	151, 1195, 1264
Bsh1236I	CGCG	1 cut(s)	276
Bsh1285I	CGRYCG	1 cut(s)	392
BshNI	GGYRCC	1 cut(s)	538
BshVI	ATCGAT	1 cut(s)	358
BsiEI	CGRYCG	1 cut(s)	392
BsiHKAI	GWGCWC	1 cut(s)	804
BsiHKCI	CYCGRG	1 cut(s)	507
BsiSI	CCGG	1 cut(s)	392
BslI	CCNNNNNNNGG	2 cut(s)	201, 507
BsmAI	GTCTC	3 cut(s)	109, 392, 701
BsmBI	CGTCTC	1 cut(s)	701
BsoBI	CYCGRG	1 cut(s)	507
Bsp119I	TTCGAA	1 cut(s)	312
Bsp1286I	GDGCHC	2 cut(s)	117, 804
Bsp1407I	TGTACA	1 cut(s)	1308
Bsp143I	GATC	2 cut(s)	432, 900
BspACI	CCGC	3 cut(s)	326, 634, 1237
BspCNI	CTCAG	3 cut(s)	171, 841, 1020
BspDI	ATCGAT	1 cut(s)	358
BspFNI	CGCG	1 cut(s)	276
BspLI	GGNNCC	2 cut(s)	343, 540
BspMI	ACCTGC	2 cut(s)	550, 1470
BspT104I	TTCGAA	1 cut(s)	312
BspT107I	GGYRCC	1 cut(s)	538
BsrDI	GCAATG	1 cut(s)	660
BsrGI	TGTACA	1 cut(s)	1308
BsrI	ACTGG	2 cut(s)	799, 1507
BssECI	CCNNGG	1 cut(s)	684
BssMI	GATC	2 cut(s)	432, 900
BssSI	CACGAG	2 cut(s)	110, 1191
Bst2BI	CACGAG	2 cut(s)	110, 1191
Bst2UI	CCWGG	1 cut(s)	126
Bst4CI	ACNGT	3 cut(s)	783, 1155, 1289
Bst6I	CTCTTC	1 cut(s)	872
BstAPI	GCANNNNNTGC	1 cut(s)	1355
BstAUI	TGTACA	1 cut(s)	1308
BstBI	TTCGAA	1 cut(s)	312
BstC8I	GCNNGC	2 cut(s)	1316, 1320
BstDEI	CTNAG	3 cut(s)	179, 849, 1007
BstF5I	GGATG	1 cut(s)	400
BstFNI	CGCG	1 cut(s)	276
BstHHI	GCGC	1 cut(s)	298
BstKTI	GATC	2 cut(s)	435, 903
BstMAI	GTCTC	3 cut(s)	109, 392, 701
BstMBI	GATC	2 cut(s)	432, 900
BstMCI	CGRYCG	1 cut(s)	392
BstMWI	GCNNNNNNNGC	2 cut(s)	1324, 1355
BstNI	CCWGG	1 cut(s)	126
BstNSI	RCATGY	1 cut(s)	1208
BstSCI	CCNGG	1 cut(s)	124
BstUI	CGCG	1 cut(s)	276
BstV1I	GCAGC	3 cut(s)	151, 1195, 1264
Bsu15I	ATCGAT	1 cut(s)	358
BsuTUI	ATCGAT	1 cut(s)	358
BtsCI	GGATG	1 cut(s)	400
BtsI	GCAGTG	1 cut(s)	291
BtsIMutI	CAGTG	3 cut(s)	291, 1311, 1416
BveI	ACCTGC	2 cut(s)	550, 1470
Cac8I	GCNNGC	2 cut(s)	1316, 1320
CaiI	CAGNNNCTG	2 cut(s)	164, 1502
CfoI	GCGC	1 cut(s)	298
ClaI	ATCGAT	1 cut(s)	358
CseI	GACGC	1 cut(s)	282
Csp6I	GTAC	2 cut(s)	539, 1309
CviAII	CATG	2 cut(s)	475, 1205
CviQI	GTAC	2 cut(s)	539, 1309
DdeI	CTNAG	3 cut(s)	179, 849, 1007
DpnI	GATC	2 cut(s)	434, 902
DpnII	GATC	2 cut(s)	432, 900
DraI	TTTAAA	2 cut(s)	456, 1126
Eam1104I	CTCTTC	1 cut(s)	872
EarI	CTCTTC	1 cut(s)	872
Eco24I	GRGCYC	1 cut(s)	117
Eco57I	CTGAAG	3 cut(s)	644, 1059, 1485
Eco88I	CYCGRG	1 cut(s)	507
EcoRI	GAATTC	2 cut(s)	469, 526
EcoRII	CCWGG	1 cut(s)	124
EcoT38I	GRGCYC	1 cut(s)	117
Esp3I	CGTCTC	1 cut(s)	701
FaeI	CATG	2 cut(s)	478, 1208
FalI	AAGNNNNNCTT	2 cut(s)	199, 231
FatI	CATG	2 cut(s)	474, 1204
FauI	CCCGC	1 cut(s)	627
FbaI	TGATCA	2 cut(s)	432, 900
Fnu4HI	GCNGC	4 cut(s)	165, 326, 1209, 1278
FokI	GGATG	1 cut(s)	407
FriOI	GRGCYC	1 cut(s)	117
Fsp4HI	GCNGC	4 cut(s)	165, 326, 1209, 1278
FspBI	CTAG	5 cut(s)	101, 1067, 1296, 1488, 1493
GlaI	GCGC	1 cut(s)	297
GluI	GCNGC	4 cut(s)	165, 326, 1209, 1278
GsuI	CTGGAG	1 cut(s)	816
HapII	CCGG	1 cut(s)	392
HgaI	GACGC	1 cut(s)	282
HhaI	GCGC	1 cut(s)	298
Hin1II	CATG	2 cut(s)	478, 1208
Hin6I	GCGC	1 cut(s)	296
HinP1I	GCGC	1 cut(s)	296
HincII	GTYRAC	1 cut(s)	1082
HindII	GTYRAC	1 cut(s)	1082
HinfI	GANTC	4 cut(s)	41, 314, 385, 572
HpaII	CCGG	1 cut(s)	392
HphI	GGTGA	3 cut(s)	455, 653, 691
Hpy166II	GTNNAC	3 cut(s)	779, 1082, 1309
Hpy188I	TCNGA	9 cut(s)	180, 304, 346, 437, 532, 663, 685, 905, 1371
Hpy188III	TCNNGA	2 cut(s)	38, 1139
Hpy8I	GTNNAC	3 cut(s)	779, 1082, 1309
HpyAV	CCTTC	3 cut(s)	309, 1330, 1334
HpyCH4III	ACNGT	3 cut(s)	783, 1155, 1289
HpyCH4IV	ACGT	1 cut(s)	1263
HpyCH4V	TGCA	5 cut(s)	653, 679, 1020, 1148, 1349
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1324, 1355
HpyF3I	CTNAG	3 cut(s)	179, 849, 1007
HpySE526I	ACGT	1 cut(s)	1263
Hsp92II	CATG	2 cut(s)	478, 1208
HspAI	GCGC	1 cut(s)	296
KpnI	GGTACC	1 cut(s)	542
Ksp22I	TGATCA	2 cut(s)	432, 900
Kzo9I	GATC	2 cut(s)	432, 900
Lsp1109I	GCAGC	3 cut(s)	151, 1195, 1264
LweI	GCATC	2 cut(s)	268, 973
MaeI	CTAG	5 cut(s)	101, 1067, 1296, 1488, 1493
MaeII	ACGT	1 cut(s)	1263
MaeIII	GTNAC	2 cut(s)	266, 974
MalI	GATC	2 cut(s)	434, 902
MboI	GATC	2 cut(s)	432, 900
MboII	GAAGA	7 cut(s)	207, 309, 874, 889, 933, 1070, 1478
MhlI	GDGCHC	2 cut(s)	117, 804
MluI	ACGCGT	1 cut(s)	274
MlyI	GAGTC	1 cut(s)	35
MmeI	TCCRAC	2 cut(s)	48, 1111
MroXI	GAANNNNTTC	1 cut(s)	8
MseI	TTAA	5 cut(s)	455, 1125, 1185, 1230, 1383
MslI	CAYNNNNRTG	1 cut(s)	896
MspI	CCGG	1 cut(s)	392
MspR9I	CCNGG	1 cut(s)	126
MvaI	CCWGG	1 cut(s)	126
MvnI	CGCG	1 cut(s)	276
MwoI	GCNNNNNNNGC	2 cut(s)	1324, 1355
NdeII	GATC	2 cut(s)	432, 900
NlaIII	CATG	2 cut(s)	478, 1208
NlaIV	GGNNCC	2 cut(s)	343, 540
NmuCI	GTSAC	2 cut(s)	266, 974
NspI	RCATGY	1 cut(s)	1208
NspV	TTCGAA	1 cut(s)	312
PdmI	GAANNNNTTC	1 cut(s)	8
PfeI	GAWTC	3 cut(s)	314, 385, 572
PflFI	GACNNNGTC	1 cut(s)	783
PkrI	GCNGC	4 cut(s)	166, 327, 1210, 1279
PleI	GAGTC	1 cut(s)	35
PpsI	GAGTC	1 cut(s)	35
PshBI	ATTAAT	1 cut(s)	1383
PsiI	TTATAA	1 cut(s)	896
Psp6I	CCWGG	1 cut(s)	124
PspGI	CCWGG	1 cut(s)	124
PspN4I	GGNNCC	2 cut(s)	343, 540
PstNI	CAGNNNCTG	2 cut(s)	164, 1502
PsyI	GACNNNGTC	1 cut(s)	783
RsaI	GTAC	2 cut(s)	540, 1310
RsaNI	GTAC	2 cut(s)	539, 1309
RseI	CAYNNNNRTG	1 cut(s)	896
SaqAI	TTAA	5 cut(s)	455, 1125, 1185, 1230, 1383
SatI	GCNGC	4 cut(s)	165, 326, 1209, 1278
Sau3AI	GATC	2 cut(s)	432, 900
SchI	GAGTC	1 cut(s)	35
ScrFI	CCNGG	1 cut(s)	126
SduI	GDGCHC	2 cut(s)	117, 804
SfaNI	GCATC	2 cut(s)	268, 973
SfuI	TTCGAA	1 cut(s)	312
SmiMI	CAYNNNNRTG	1 cut(s)	896
SmlI	CTYRAG	1 cut(s)	254
SmoI	CTYRAG	1 cut(s)	254
SsiI	CCGC	3 cut(s)	326, 634, 1237
SspI	AATATT	2 cut(s)	1107, 1366
SspMI	CTAG	5 cut(s)	101, 1067, 1296, 1488, 1493
StyD4I	CCNGG	1 cut(s)	124
TaaI	ACNGT	3 cut(s)	783, 1155, 1289
TaiI	ACGT	1 cut(s)	1266
TaqI	TCGA	5 cut(s)	39, 312, 358, 388, 516
TaqII	GACCGA	1 cut(s)	1388
TatI	WGTACW	1 cut(s)	1308
TauI	GCSGC	1 cut(s)	328
TfiI	GAWTC	3 cut(s)	314, 385, 572
Tru1I	TTAA	5 cut(s)	455, 1125, 1185, 1230, 1383
Tru9I	TTAA	5 cut(s)	455, 1125, 1185, 1230, 1383
TscAI	CASTG	3 cut(s)	298, 1311, 1416
TseFI	GTSAC	2 cut(s)	266, 974
TseI	GCWGC	3 cut(s)	164, 1208, 1277
Tsp45I	GTSAC	2 cut(s)	266, 974
TspDTI	ATGAA	7 cut(s)	17, 62, 491, 615, 875, 1071, 1349
TspGWI	ACGGA	1 cut(s)	285
TspRI	CASTG	3 cut(s)	298, 1311, 1416
Tth111I	GACNNNGTC	1 cut(s)	783
VspI	ATTAAT	1 cut(s)	1383
XapI	RAATTY	7 cut(s)	131, 223, 469, 526, 690, 925, 1031
XceI	RCATGY	1 cut(s)	1208
XcmI	CCANNNNNNNNNTGG	1 cut(s)	710
XmnI	GAANNNNTTC	1 cut(s)	8
XspI	CTAG	5 cut(s)	101, 1067, 1296, 1488, 1493

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.