MD08G1229100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010890
Physical Location & Seq
Forward (+)
29525947 .. 29526390
444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1229100.v1.1.491

Sequence Viewer

Length: 444 bp
ATGGAAGATCTCATTGCCCAATTCAGCTTCCTCTCCAACCAAGCCTTGCAAGACAAAACCTTCGACCCCTCGACGATCGAGGACCTCATGAAGCTGTTCGAGCTCGAAGCCTACAACTCGTGGGCCGCAGTCGAGCTTGAACAGCAACAAGAGCTGGAAGAAGCTGAGCGTGCCATGCAGGAAGCGGAGGAGTACATGGACTCGGTGATGGAGAGCGCCATGGACGAGTTCAGACGCTTCGAGGAGGAGATGGAACGCATGTCGAAAGCGGAATTGGACAAACTGGAGGCGACGGCGGAAGGGGCTAGGAGGATGGGGATGGTGGTGGAAAATGCAGCAACTGTTGCTGCCAAGAGGTATATTGAGGCTGCACTCAACTCTGCTACTGCTTCAATGAAATCGGCTTGGAAGGGCATTTCTGGTAAGAAGGTTCACCCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

16.68

Weight (kDa)

4.45

Isoelectric Point (pI)

51.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60870
fragaria_vesca FvH4_5g29530 FvH4_5g29530
malus_domestica MD08G1229100.v1.1 MD15G1423100.v1.1
prunus_persica Prupe.1G568400_v2.0.a1
pyrus_communis pycom15g37490 pycom15g37510
rosa_chinensis RchiOBHm_Chr7g0222451
rosa_laevigata RLG00000002058
rosa_multiflora Rmu_sc0004638.1_g000013 Rmu_sc0035136.1_g000001
rosa_roxburghii Rroxscaffold_3G00236960
rosa_rugosa Rorug07G0208800
rosa_samantha Rh7AG350900 Rh7BG341800 Rh7CG368200 Rh7DG347800
rosa_wichuraiana Rw7G029640 Rw7G029980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 126, 185, 269, 296
AfaI GTAC 1 cut(s) 194
AgsI TTSAA 2 cut(s) 140, 393
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AluBI AGCT 6 cut(s) 27, 94, 103, 136, 154, 164
AluI AGCT 6 cut(s) 27, 94, 103, 136, 154, 164
Alw21I GWGCWC 1 cut(s) 105
AlwNI CAGNNNCTG 1 cut(s) 341
AoxI GGCC 1 cut(s) 123
ApeKI GCWGC 3 cut(s) 335, 347, 368
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
Asp700I GAANNNNTTC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 218
AspS9I GGNCC 2 cut(s) 82, 123
AsuHPI GGTGA 2 cut(s) 217, 425
AvaII GGWCC 1 cut(s) 82
BanII GRGCYC 1 cut(s) 105
BauI CACGAG 1 cut(s) 118
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 3 cut(s) 334, 347, 355
BccI CCATC 4 cut(s) 202, 244, 307, 313
BceAI ACGGC 1 cut(s) 309
BfaI CTAG 1 cut(s) 306
BfoI RGCGCY 1 cut(s) 219
BglII AGATCT 1 cut(s) 7
BisI GCNGC 4 cut(s) 126, 336, 348, 369
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 4 cut(s) 127, 337, 349, 370
Bme18I GGWCC 1 cut(s) 82
BmgT120I GGNCC 2 cut(s) 82, 123
BpmI CTGGAG 1 cut(s) 305
Bpu1102I GCTNAGC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 219
Bse1I ACTGG 1 cut(s) 288
Bse3DI GCAATG 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 2 cut(s) 318, 324
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 288
BseRI GAGGAG 3 cut(s) 203, 257, 260
BseXI GCAGC 3 cut(s) 334, 347, 355
BsgI GTGCAG 1 cut(s) 354
Bsh1285I CGRYCG 1 cut(s) 78
BshFI GGCC 1 cut(s) 125
BsiEI CGRYCG 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 105
BsnI GGCC 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 2 cut(s) 7, 75
Bsp1720I GCTNAGC 1 cut(s) 165
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 4 cut(s) 126, 185, 269, 296
BspANI GGCC 1 cut(s) 125
BspCNI CTCAG 1 cut(s) 157
BspHI TCATGA 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 1 cut(s) 288
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 2 cut(s) 7, 75
BssSI CACGAG 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 219
Bst2BI CACGAG 1 cut(s) 118
Bst4CI ACNGT 1 cut(s) 343
BstAPI GCANNNNNTGC 1 cut(s) 344
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 1 cut(s) 165
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 2 cut(s) 318, 324
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 2 cut(s) 10, 78
BstMBI GATC 2 cut(s) 7, 75
BstMCI CGRYCG 1 cut(s) 78
BstMWI GCNNNNNNNGC 7 cut(s) 100, 142, 151, 170, 175, 302, 344
BstNSI RCATGY 1 cut(s) 262
BstV1I GCAGC 3 cut(s) 334, 347, 355
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 1 cut(s) 125
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 2 cut(s) 318, 324
Cac8I GCNNGC 1 cut(s) 171
CaiI CAGNNNCTG 1 cut(s) 341
CciI TCATGA 1 cut(s) 87
CfoI GCGC 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 82, 123
CseI GACGC 1 cut(s) 243
Csp6I GTAC 1 cut(s) 193
CviAII CATG 5 cut(s) 88, 175, 196, 220, 259
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 1 cut(s) 165
DpnI GATC 2 cut(s) 9, 77
DpnII GATC 2 cut(s) 7, 75
EciI GGCGGA 1 cut(s) 311
Ecl136II GAGCTC 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 82
Eco53kI GAGCTC 1 cut(s) 103
EcoICRI GAGCTC 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 82
EcoT14I CCWWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 5 cut(s) 91, 178, 199, 223, 262
FaiI YATR 6 cut(s) 89, 176, 197, 221, 260, 360
FatI CATG 5 cut(s) 87, 174, 195, 219, 258
Fnu4HI GCNGC 4 cut(s) 126, 336, 348, 369
FokI GGATG 2 cut(s) 325, 331
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 4 cut(s) 126, 336, 348, 369
FspBI CTAG 1 cut(s) 306
GlaI GCGC 1 cut(s) 217
GluI GCNGC 4 cut(s) 126, 336, 348, 369
GsuI CTGGAG 1 cut(s) 305
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 1 cut(s) 125
HgaI GACGC 1 cut(s) 243
HhaI GCGC 1 cut(s) 218
Hin1II CATG 5 cut(s) 91, 178, 199, 223, 262
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HinfI GANTC 1 cut(s) 200
HphI GGTGA 2 cut(s) 217, 425
Hpy166II GTNNAC 1 cut(s) 433
Hpy188I TCNGA 1 cut(s) 233
Hpy188III TCNNGA 1 cut(s) 88
Hpy8I GTNNAC 1 cut(s) 433
Hpy99I CGWCG 2 cut(s) 76, 295
HpyAV CCTTC 4 cut(s) 70, 293, 403, 421
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4V TGCA 4 cut(s) 49, 178, 335, 371
HpyF10VI GCNNNNNNNGC 7 cut(s) 100, 142, 151, 170, 175, 302, 344
HpyF3I CTNAG 1 cut(s) 165
Hsp92II CATG 5 cut(s) 91, 178, 199, 223, 262
HspAI GCGC 1 cut(s) 216
Kzo9I GATC 2 cut(s) 7, 75
LpnPI CCDG 4 cut(s) 140, 164, 269, 405
Lsp1109I GCAGC 3 cut(s) 334, 347, 355
MaeI CTAG 1 cut(s) 306
MalI GATC 2 cut(s) 9, 77
MboI GATC 2 cut(s) 7, 75
MboII GAAGA 2 cut(s) 17, 170
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 2 cut(s) 20, 272
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 1 cut(s) 60
MroXI GAANNNNTTC 1 cut(s) 95
MseI TTAA 1 cut(s) 442
MwoI GCNNNNNNNGC 7 cut(s) 100, 142, 151, 170, 175, 302, 344
NcoI CCATGG 1 cut(s) 219
NdeII GATC 2 cut(s) 7, 75
NlaIII CATG 5 cut(s) 91, 178, 199, 223, 262
NspI RCATGY 1 cut(s) 262
PagI TCATGA 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 95
PkrI GCNGC 4 cut(s) 127, 337, 349, 370
Ple19I CGATCG 1 cut(s) 78
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PpuMI RGGWCCY 1 cut(s) 82
Psp124BI GAGCTC 1 cut(s) 105
Psp5II RGGWCCY 1 cut(s) 82
PspPI GGNCC 2 cut(s) 82, 123
PspPPI RGGWCCY 1 cut(s) 82
PstNI CAGNNNCTG 1 cut(s) 341
PsuI RGATCY 1 cut(s) 7
PvuI CGATCG 1 cut(s) 78
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
SacI GAGCTC 1 cut(s) 105
SaqAI TTAA 1 cut(s) 442
SatI GCNGC 4 cut(s) 126, 336, 348, 369
Sau3AI GATC 2 cut(s) 7, 75
Sau96I GGNCC 2 cut(s) 82, 123
SchI GAGTC 1 cut(s) 194
SduI GDGCHC 1 cut(s) 105
SinI GGWCC 1 cut(s) 82
Sse9I AATT 2 cut(s) 20, 272
SsiI CCGC 4 cut(s) 126, 185, 269, 296
SspMI CTAG 1 cut(s) 306
SstI GAGCTC 1 cut(s) 105
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 343
TaqI TCGA 8 cut(s) 63, 71, 78, 99, 105, 132, 240, 263
TasI AATT 2 cut(s) 20, 272
TatI WGTACW 1 cut(s) 192
TauI GCSGC 1 cut(s) 128
Tru1I TTAA 1 cut(s) 442
Tru9I TTAA 1 cut(s) 442
TseI GCWGC 3 cut(s) 335, 347, 368
TspDTI ATGAA 2 cut(s) 104, 410
VpaK11BI GGWCC 1 cut(s) 82
XceI RCATGY 1 cut(s) 262
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.