MD15G1423100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010890
Physical Location & Seq
Forward (+)
52454341 .. 52454784
444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1423100.v1.1.491

Sequence Viewer

Length: 444 bp
ATGGAAGATCTCATTGCTCAATTCAGCTTCCTCTCCAACGAAGCCTTGCATGACAAATCCTTCGACCCCTCCACGGTCGAGGACCTCATGAAGCTGTTCGAGATCGAAGCCTACAGATCGTGGGCCGCGGTCGAGCTCGAACAGCAACAGGAGGTGGAAGAAGCCGAGACTGCCATGCAGGAAGCCGAGGACTACATGGACTCGGTGATGGAGAGCGCCATGGACGAGTTCCGACGCTTCGAGGAGGAGATGGAACGCATGTCGAAAGAGGAAATGGACAGCCTGGTTGCGACGGCGGAAGGGGCTAGGAGGATGGGGAAGTTGATGGAAAATGCAGCAACTGTTGCTTCCAAGAGGTACATTGAGGCTGCACTCAACTCCGCCACTGCTTCCATGAAATCGGCTTGGAAGGGCATTTCTACCAAGAAGGTTCACCCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

16.73

Weight (kDa)

4.47

Isoelectric Point (pI)

54.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010890)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G60870
fragaria_vesca FvH4_5g29530 FvH4_5g29530
malus_domestica MD08G1229100.v1.1 MD15G1423100.v1.1
prunus_persica Prupe.1G568400_v2.0.a1
pyrus_communis pycom15g37490 pycom15g37510
rosa_chinensis RchiOBHm_Chr7g0222451
rosa_laevigata RLG00000002058
rosa_multiflora Rmu_sc0004638.1_g000013 Rmu_sc0035136.1_g000001
rosa_roxburghii Rroxscaffold_3G00236960
rosa_rugosa Rorug07G0208800
rosa_samantha Rh7AG350900 Rh7BG341800 Rh7CG368200 Rh7DG347800
rosa_wichuraiana Rw7G029640 Rw7G029980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 128
AciI CCGC 4 cut(s) 126, 128, 296, 381
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 1 cut(s) 73
AjnI CCWGG 1 cut(s) 282
AluBI AGCT 3 cut(s) 27, 94, 136
AluI AGCT 3 cut(s) 27, 94, 136
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 1 cut(s) 161
AlwNI CAGNNNCTG 1 cut(s) 341
AoxI GGCC 1 cut(s) 123
ApeKI GCWGC 2 cut(s) 335, 368
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
Asp700I GAANNNNTTC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 218
AspS9I GGNCC 2 cut(s) 82, 123
AsuHPI GGTGA 2 cut(s) 217, 425
AvaII GGWCC 1 cut(s) 82
BanII GRGCYC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 347, 355
BccI CCATC 4 cut(s) 202, 244, 307, 319
BceAI ACGGC 1 cut(s) 309
BciT130I CCWGG 1 cut(s) 284
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 1 cut(s) 306
BfmI CTRYAG 1 cut(s) 112
BfoI RGCGCY 1 cut(s) 219
BglII AGATCT 1 cut(s) 7
BisI GCNGC 3 cut(s) 126, 336, 369
BlsI GCNGC 3 cut(s) 127, 337, 370
Bme1390I CCNGG 1 cut(s) 284
Bme18I GGWCC 1 cut(s) 82
BmgT120I GGNCC 2 cut(s) 82, 123
BmrFI CCNGG 1 cut(s) 284
BsaJI CCNNGG 4 cut(s) 72, 126, 186, 219
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 1 cut(s) 284
BseDI CCNNGG 4 cut(s) 72, 126, 186, 219
BseGI GGATG 1 cut(s) 318
BseLI CCNNNNNNNGG 1 cut(s) 73
BseMI GCAATG 1 cut(s) 12
BseRI GAGGAG 2 cut(s) 257, 260
BseXI GCAGC 2 cut(s) 347, 355
BsgI GTGCAG 1 cut(s) 354
Bsh1236I CGCG 1 cut(s) 128
Bsh1285I CGRYCG 2 cut(s) 78, 132
BshFI GGCC 1 cut(s) 125
BsiEI CGRYCG 2 cut(s) 78, 132
BsiHKAI GWGCWC 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 1 cut(s) 161
BsnI GGCC 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 3 cut(s) 7, 102, 116
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 4 cut(s) 126, 128, 296, 381
BspANI GGCC 1 cut(s) 125
BspFNI CGCG 1 cut(s) 128
BspHI TCATGA 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 12
BssECI CCNNGG 4 cut(s) 72, 126, 186, 219
BssMI GATC 3 cut(s) 7, 102, 116
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 2 cut(s) 76, 343
BstAPI GCANNNNNTGC 1 cut(s) 344
BstDSI CCRYGG 3 cut(s) 72, 126, 219
BstF5I GGATG 1 cut(s) 318
BstFNI CGCG 1 cut(s) 128
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 3 cut(s) 10, 105, 119
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 3 cut(s) 7, 102, 116
BstMCI CGRYCG 2 cut(s) 78, 132
BstMWI GCNNNNNNNGC 4 cut(s) 142, 170, 302, 344
BstNI CCWGG 1 cut(s) 284
BstNSI RCATGY 1 cut(s) 262
BstSCI CCNGG 1 cut(s) 282
BstSFI CTRYAG 1 cut(s) 112
BstUI CGCG 1 cut(s) 128
BstV1I GCAGC 2 cut(s) 347, 355
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 1 cut(s) 125
BtgI CCRYGG 3 cut(s) 72, 126, 219
BtsCI GGATG 1 cut(s) 318
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 1 cut(s) 384
CaiI CAGNNNCTG 1 cut(s) 341
CciI TCATGA 1 cut(s) 87
CfoI GCGC 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 82, 123
Cfr42I CCGCGG 1 cut(s) 129
CseI GACGC 1 cut(s) 243
Csp6I GTAC 1 cut(s) 358
CviAII CATG 7 cut(s) 50, 88, 175, 196, 220, 259, 394
CviQI GTAC 1 cut(s) 358
DpnI GATC 3 cut(s) 9, 104, 118
DpnII GATC 3 cut(s) 7, 102, 116
EciI GGCGGA 2 cut(s) 311, 370
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 82
Eco53kI GAGCTC 1 cut(s) 136
EcoICRI GAGCTC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 282
EcoT14I CCWWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 7 cut(s) 53, 91, 178, 199, 223, 262, 397
FaiI YATR 7 cut(s) 51, 89, 176, 197, 221, 260, 395
FatI CATG 7 cut(s) 49, 87, 174, 195, 219, 258, 393
Fnu4HI GCNGC 3 cut(s) 126, 336, 369
FokI GGATG 1 cut(s) 325
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 3 cut(s) 126, 336, 369
FspBI CTAG 1 cut(s) 306
GlaI GCGC 1 cut(s) 217
GluI GCNGC 3 cut(s) 126, 336, 369
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 1 cut(s) 125
HgaI GACGC 1 cut(s) 243
HhaI GCGC 1 cut(s) 218
Hin1II CATG 7 cut(s) 53, 91, 178, 199, 223, 262, 397
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HinfI GANTC 1 cut(s) 200
HphI GGTGA 2 cut(s) 217, 425
Hpy166II GTNNAC 1 cut(s) 433
Hpy188I TCNGA 1 cut(s) 233
Hpy188III TCNNGA 3 cut(s) 88, 100, 441
Hpy8I GTNNAC 1 cut(s) 433
Hpy99I CGWCG 2 cut(s) 237, 295
HpyAV CCTTC 4 cut(s) 70, 293, 403, 421
HpyCH4III ACNGT 2 cut(s) 76, 343
HpyCH4V TGCA 4 cut(s) 49, 178, 335, 371
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 170, 302, 344
Hsp92II CATG 7 cut(s) 53, 91, 178, 199, 223, 262, 397
HspAI GCGC 1 cut(s) 216
KspI CCGCGG 1 cut(s) 129
Kzo9I GATC 3 cut(s) 7, 102, 116
LpnPI CCDG 4 cut(s) 134, 164, 269, 296
Lsp1109I GCAGC 2 cut(s) 347, 355
MaeI CTAG 1 cut(s) 306
MalI GATC 3 cut(s) 9, 104, 118
MboI GATC 3 cut(s) 7, 102, 116
MboII GAAGA 2 cut(s) 17, 170
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 1 cut(s) 138
MluCI AATT 1 cut(s) 20
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 2 cut(s) 60, 256
MroXI GAANNNNTTC 1 cut(s) 95
MspA1I CMGCKG 1 cut(s) 128
MspR9I CCNGG 1 cut(s) 284
MvaI CCWGG 1 cut(s) 284
MvnI CGCG 1 cut(s) 128
MwoI GCNNNNNNNGC 4 cut(s) 142, 170, 302, 344
NcoI CCATGG 1 cut(s) 219
NdeII GATC 3 cut(s) 7, 102, 116
NlaIII CATG 7 cut(s) 53, 91, 178, 199, 223, 262, 397
NmeAIII GCCGAG 2 cut(s) 190, 211
NspI RCATGY 1 cut(s) 262
PagI TCATGA 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 95
PkrI GCNGC 3 cut(s) 127, 337, 370
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PpuMI RGGWCCY 1 cut(s) 82
Psp124BI GAGCTC 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 82
Psp6I CCWGG 1 cut(s) 282
PspGI CCWGG 1 cut(s) 282
PspPI GGNCC 2 cut(s) 82, 123
PspPPI RGGWCCY 1 cut(s) 82
PstNI CAGNNNCTG 1 cut(s) 341
PsuI RGATCY 1 cut(s) 7
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SacI GAGCTC 1 cut(s) 138
SacII CCGCGG 1 cut(s) 129
SatI GCNGC 3 cut(s) 126, 336, 369
Sau3AI GATC 3 cut(s) 7, 102, 116
Sau96I GGNCC 2 cut(s) 82, 123
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 1 cut(s) 284
SduI GDGCHC 1 cut(s) 138
SetI ASST 7 cut(s) 29, 87, 96, 138, 156, 359, 432
SfcI CTRYAG 1 cut(s) 112
Sfr303I CCGCGG 1 cut(s) 129
SgrBI CCGCGG 1 cut(s) 129
SinI GGWCC 1 cut(s) 82
Sse9I AATT 1 cut(s) 20
SsiI CCGC 4 cut(s) 126, 128, 296, 381
SspMI CTAG 1 cut(s) 306
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 1 cut(s) 282
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 2 cut(s) 76, 343
TaqI TCGA 8 cut(s) 63, 78, 99, 105, 132, 138, 240, 263
TasI AATT 1 cut(s) 20
TauI GCSGC 1 cut(s) 128
TscAI CASTG 1 cut(s) 391
TseI GCWGC 2 cut(s) 335, 368
TspDTI ATGAA 2 cut(s) 104, 410
TspRI CASTG 1 cut(s) 391
VpaK11BI GGWCC 1 cut(s) 82
XceI RCATGY 1 cut(s) 262
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.