Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0005566

Physical Location & Seq

Genomic Coordinates Reverse (-)

30964720 .. 30970346

Gene Structure 5627 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1244800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGCATTGGCTGCAAAAATCGCGGCCTGAGAAATGGCGTTTGGCGTTGGGAATTAGACGAATTTTGCAGTGTTCTTGTTGGAATAATAGGGGACTGCGAAGTAGAATAATGGGCTGCATTTGCTCAAAAGGAAGTTTATCCAATGAATATGTTTCTGAGAATCATCGTAGAGACAAAGAACATAAATCAAACAAGTCATCAAAACGGTCTGAAGCTGCAGTTGGGGCTGATGCAACTGCGCGGTTGATAGCCAATCCTTGCAAAGAAGACAATGCAGGGTCAACTCCTATTTCATCAGATGAAGGGGAGAAGATGGTGGATGCTGAGAAACCCACAAAACCCTTCACGGAGGGTGGGGCAAGTGGGGGAAAGTTGCCGTCTGGGCTGACTAGGATAACCAGTGTAACAAATGGAGAAAGAGGAGCACAAGTTGTTGCTGGATGGCCTTCTTGGTTGACAGCAGTGGCCGGCGAAGCGATCAGTGGATGGGTGCCTCTCAAGGCAGAGTCATTTGAGAAATTGGAGAAGATTGGACAAGGAACTTACAGCAGTGTGTACAGAGCCCGTGATCTTGATTCAAACAAAATAGTTGCATTGAAGAAGGTGCGATTTGCCAATATGGATCCGGAAAGTGTGCGATTTATGGCGAGAGAAATTCTTATTTTGCGTAGGCTTGATCACCCCAACGTCATGAAGCTTGAAGGTCTGATTACATCGAAGGTTTCGGGCAGCTTATACCTGGTCTTTGAGTACATGGAGCATGATCTTGTAGGGATTGCAGCACGACCTGGAGTCAAGTTCACTGAAGCACAGATTAAATGTTACATGCAACAGCTTCTTTGTGGACTTGAGCATTGTCACAGTCATGGTATCTTGCACCGTGATATCAAGGGATCAAATCTTTTGATTGACTACCATGGCAAGCTAAAGATTGCTGACTTTGGACTAGCAACCTTTTACAGACCCCGTCAAAAGCAGCCTCTGACAAGCCGGGTAGTAACCTTGTGGTACAGACCACCTGAACTTCTGCTTGGCTCTACAGATTATGGGGTTGCTGTGGATTTGTGGAGTGCTGGTTGTATTCTTGCAGAATTATTTGCTGGGAAGCCTATCATGCCAGGAAGAACAGAGGTGGAGCAACTACATAAGATATTTAAACTTTGTGGCTCACCTTGTGAGGAATACTGGAAGAGATCAAAATTGCCACATGCAACTATTTTTAAACCTACACATCCATACAAGCGATGTGTTGCTGAGACATACAAGGACTTCCCTCGATCTTCATTGGCCCTGTTAGAGGTTCTTCTAGCTGTAGAACCTGAAGGTCGGGGAACAGCTTCTTCTGCCCTTCAGAGTGAGTTCTTCACATCTTTGCCTCTTCCCTGTGATCCATCAAGTTTGCCTAAGTACCCACCAAGTAAGGAGTTTGATGCCAAGCTCCGAGACGAGGAAGCTAGAAGGCGAAAAGCAGCTAGTGGTAAAGGAGGGAGTTCCAAAGACTCAAAGGCTGTGCCAGCATCAGATGCCAATGCAATACAGCGACAAGGGCAGACTAACCCCAAAAGCATCAGTGAGAAGTACAATCCTGAAGAGGATGGTGGCTCTGGCTTTCCTATCGAGCCTCCCAAGGGAGCAGCATCAAATGCCTTCTCCCATTCTGGCCAGTCAGTGCACCCAAGCGCATTTGAATCTTTGCAATCTGGCCATTCTAAGAATGGATCTTACATGTATCGTGGAGCAGCCCAGTCGTCGAGGTTTTCTAATTCAGCTTCAGTTCGAAGTGGTTCACGATTTGATGGTATCGCAGAAAGTGCGAATCCACACTGGCCAGAGGAGCGTAAGAATGCCAGATATAACCATTTAGACAACGGGGAGTCCTCTGTGAAACATGATTGGTCACACCATCTTTTGGAAAGGCCAAAGTCTTCACATAAGAAGGATGAACAGCCATCTGGGAAGGAGTATGCAACGGGTTACGGTAACAAAAAGAACCGGATTCACTACTCTGGACCATTGATGCACCCTGGAGGAAACCTTGAGGAGATGCTCAAAGAGCATGAGAAACAAATACAACATGCTGTCCGCAAAGCTCGTCTAGACAAGACCAAAGCCAAGAAAGCTAACAGTGACAATGGACAAACAGAATCCCTACTTCAGCATGTAAGAAACGGCAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

726

Amino Acids

80.38

Weight (kDa)

9.41

Isoelectric Point (pI)

48.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	171 - 455	9.2e-67	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	173 - 386	3.3e-30	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005566)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G57700
fragaria_vesca	FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28310
malus_domestica	MD08G1244800.v1.1 MD15G1439900.v1.1
prunus_persica	Prupe.1G582200_v2.0.a1 Prupe.1G582200_v2.0.a1 Prupe.1G582200_v2.0.a1 Prupe.1G582200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0220331
rosa_laevigata	RLG00000002229
rosa_multiflora	Rmu_sc0001136.1_g000085
rosa_roxburghii	Rroxscaffold_3G00239520
rosa_rugosa	Rorug07G0192900
rosa_samantha	Rh7AG334200 Rh7BG325100 Rh7CG351900 Rh7DG330900
rosa_wichuraiana	Rw7G028280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2163
Acc36I	ACCTGC	1 cut(s)	2163
AccB1I	GGYRCC	1 cut(s)	490
AccB7I	CCANNNNNTGG	1 cut(s)	1909
AccII	CGCG	2 cut(s)	22, 241
AccIII	TCCGGA	1 cut(s)	625
AciI	CCGC	3 cut(s)	22, 241, 2083
AclWI	GGATC	5 cut(s)	617, 630, 901, 1382, 1726
AcoI	YGGCCR	4 cut(s)	465, 1660, 1702, 1826
AcsI	RAATTY	2 cut(s)	60, 654
AcuI	CTGAAG	7 cut(s)	231, 825, 1334, 1341, 1608, 1755, 2138
AdeI	CACNNNGTG	1 cut(s)	1175
AfaI	GTAC	5 cut(s)	557, 752, 1010, 1409, 1580
AfiI	CCNNNNNNNGG	4 cut(s)	1297, 1447, 1628, 1909
AflIII	ACRYGT	1 cut(s)	1725
AgsI	TTSAA	4 cut(s)	579, 598, 701, 1688
AhdI	GACNNNNNGTC	1 cut(s)	791
AjnI	CCWGG	4 cut(s)	738, 787, 1117, 2023
AjuI	GAANNNNNNNTTGG	6 cut(s)	23, 55, 204, 236, 1014, 1046
Alw21I	GWGCWC	2 cut(s)	427, 1674
Alw26I	GTCTC	3 cut(s)	165, 1250, 1437
Alw44I	GTGCAC	1 cut(s)	1670
AlwI	GGATC	5 cut(s)	617, 630, 901, 1382, 1726
AlwNI	CAGNNNCTG	1 cut(s)	982
Aor13HI	TCCGGA	1 cut(s)	625
AoxI	GGCC	8 cut(s)	23, 443, 465, 1287, 1660, 1702, 1826, 1916
ApaLI	GTGCAC	1 cut(s)	1670
ApeKI	GCWGC	9 cut(s)	10, 114, 215, 729, 779, 976, 1469, 1634, 1739
ApoI	RAATTY	2 cut(s)	60, 654
Asp700I	GAANNNNTTC	2 cut(s)	1336, 1783
AspLEI	GCGC	2 cut(s)	241, 1682
AspS9I	GGNCC	2 cut(s)	1288, 2009
AsuC2I	CCSGG	1 cut(s)	992
AsuHPI	GGTGA	2 cut(s)	671, 1161
AsuII	TTCGAA	1 cut(s)	1777
AvaII	GGWCC	1 cut(s)	2009
BaeGI	GKGCMC	1 cut(s)	1674
BalI	TGGCCA	3 cut(s)	1662, 1704, 1828
BamHI	GGATCC	1 cut(s)	622
BanI	GGYRCC	1 cut(s)	490
BanII	GRGCYC	1 cut(s)	565
BbsI	GAAGAC	2 cut(s)	273, 1917
Bbv12I	GWGCWC	2 cut(s)	427, 1674
BbvI	GCAGC	8 cut(s)	101, 202, 741, 791, 988, 1481, 1646, 1751
BccI	CCATC	8 cut(s)	307, 435, 480, 1399, 1589, 1790, 1911, 1957
BceAI	ACGGC	1 cut(s)	361
BcgI	CGANNNNNNTGC	2 cut(s)	1728, 1762
BciT130I	CCWGG	4 cut(s)	740, 789, 1119, 2025
BclI	TGATCA	1 cut(s)	676
BcnI	CCSGG	1 cut(s)	992
BcoDI	GTCTC	3 cut(s)	165, 1250, 1437
BfaI	CTAG	6 cut(s)	390, 947, 1307, 1455, 1473, 2096
BfmI	CTRYAG	3 cut(s)	216, 1038, 1311
BfuAI	ACCTGC	1 cut(s)	2163
BglI	GCCNNNNNGGC	1 cut(s)	382
Bme1390I	CCNGG	5 cut(s)	740, 789, 992, 1119, 2025
Bme18I	GGWCC	1 cut(s)	2009
BmeRI	GACNNNNNGTC	1 cut(s)	791
BmgT120I	GGNCC	2 cut(s)	1288, 2009
BmiI	GGNNCC	2 cut(s)	492, 624
BmrFI	CCNGG	5 cut(s)	740, 789, 992, 1119, 2025
BmrI	ACTGGG	1 cut(s)	1738
BmsI	GCATC	9 cut(s)	220, 310, 1420, 1513, 1526, 1575, 1646, 2007, 2034
BmuI	ACTGGG	1 cut(s)	1738
BpiI	GAAGAC	2 cut(s)	273, 1917
BpmI	CTGGAG	2 cut(s)	810, 2046
Bpu14I	TTCGAA	1 cut(s)	1777
BpuEI	CTTGAG	3 cut(s)	482, 869, 2057
BpuMI	CCSGG	1 cut(s)	992
BsaJI	CCNNGG	3 cut(s)	916, 1626, 2023
BsaWI	WCCGGW	2 cut(s)	625, 1992
BsaXI	ACNNNNNCTCC	4 cut(s)	414, 444, 1060, 1090
Bsc4I	CCNNNNNNNGG	4 cut(s)	1297, 1447, 1628, 1909
Bse118I	RCCGGY	1 cut(s)	467
Bse1I	ACTGG	5 cut(s)	399, 1190, 1663, 1744, 1829
BseAI	TCCGGA	1 cut(s)	625
BseBI	CCWGG	4 cut(s)	740, 789, 1119, 2025
BseDI	CCNNGG	3 cut(s)	916, 1626, 2023
BseGI	GGATG	6 cut(s)	325, 446, 491, 1231, 1600, 1945
BseLI	CCNNNNNNNGG	4 cut(s)	1297, 1447, 1628, 1909
BseMII	CTCAG	4 cut(s)	18, 147, 315, 1245
BseNI	ACTGG	5 cut(s)	399, 1190, 1663, 1744, 1829
BseRI	GAGGAG	3 cut(s)	435, 1847, 2054
BseSI	GKGCMC	1 cut(s)	1674
BseXI	GCAGC	8 cut(s)	101, 202, 741, 791, 988, 1481, 1646, 1751
BseYI	CCCAGC	1 cut(s)	1100
Bsh1236I	CGCG	2 cut(s)	22, 241
BshFI	GGCC	8 cut(s)	25, 445, 467, 1289, 1662, 1704, 1828, 1918
BshNI	GGYRCC	1 cut(s)	490
BsiHKAI	GWGCWC	2 cut(s)	427, 1674
BsiSI	CCGG	4 cut(s)	468, 626, 991, 1993
BslFI	GGGAC	1 cut(s)	105
BslI	CCNNNNNNNGG	4 cut(s)	1297, 1447, 1628, 1909
BsmAI	GTCTC	3 cut(s)	165, 1250, 1437
BsmBI	CGTCTC	1 cut(s)	1437
BsmFI	GGGAC	1 cut(s)	105
BsmI	GAATGC	1 cut(s)	1849
BsnI	GGCC	8 cut(s)	25, 445, 467, 1289, 1662, 1704, 1828, 1918
Bsp119I	TTCGAA	1 cut(s)	1777
Bsp1286I	GDGCHC	3 cut(s)	427, 565, 1674
Bsp13I	TCCGGA	1 cut(s)	625
Bsp1407I	TGTACA	1 cut(s)	555
Bsp19I	CCATGG	1 cut(s)	916
BspACI	CCGC	3 cut(s)	22, 241, 2083
BspANI	GGCC	8 cut(s)	25, 445, 467, 1289, 1662, 1704, 1828, 1918
BspCNI	CTCAG	4 cut(s)	19, 148, 316, 1246
BspEI	TCCGGA	1 cut(s)	625
BspFNI	CGCG	2 cut(s)	22, 241
BspHI	TCATGA	1 cut(s)	690
BspLI	GGNNCC	2 cut(s)	492, 624
BspMAI	CTGCAG	1 cut(s)	220
BspMI	ACCTGC	1 cut(s)	2163
BspPI	GGATC	5 cut(s)	617, 630, 901, 1382, 1726
BspT104I	TTCGAA	1 cut(s)	1777
BspT107I	GGYRCC	1 cut(s)	490
BsrFI	RCCGGY	1 cut(s)	467
BsrGI	TGTACA	1 cut(s)	555
BsrI	ACTGG	5 cut(s)	399, 1190, 1663, 1744, 1829
BssAI	RCCGGY	1 cut(s)	467
BssECI	CCNNGG	3 cut(s)	916, 1626, 2023
BssT1I	CCWWGG	2 cut(s)	916, 1626
Bst2UI	CCWGG	4 cut(s)	740, 789, 1119, 2025
Bst4CI	ACNGT	5 cut(s)	207, 863, 881, 1979, 2126
Bst6I	CTCTTC	3 cut(s)	1184, 1383, 1584
BstAPI	GCANNNNNTGC	4 cut(s)	10, 1523, 1643, 1811
BstAUI	TGTACA	1 cut(s)	555
BstBI	TTCGAA	1 cut(s)	1777
BstC8I	GCNNGC	3 cut(s)	469, 923, 1515
BstDEI	CTNAG	6 cut(s)	27, 156, 324, 1254, 1404, 1710
BstDSI	CCRYGG	1 cut(s)	916
BstENI	CCTNNNNNAGG	1 cut(s)	1295
BstF5I	GGATG	6 cut(s)	325, 446, 491, 1231, 1600, 1945
BstFNI	CGCG	2 cut(s)	22, 241
BstHHI	GCGC	2 cut(s)	241, 1682
BstMAI	GTCTC	3 cut(s)	165, 1250, 1437
BstNI	CCWGG	4 cut(s)	740, 789, 1119, 2025
BstNSI	RCATGY	5 cut(s)	829, 1211, 1729, 2078, 2162
BstSCI	CCNGG	5 cut(s)	738, 787, 990, 1117, 2023
BstSFI	CTRYAG	3 cut(s)	216, 1038, 1311
BstSLI	GKGCMC	1 cut(s)	1674
BstUI	CGCG	2 cut(s)	22, 241
BstV1I	GCAGC	8 cut(s)	101, 202, 741, 791, 988, 1481, 1646, 1751
BstV2I	GAAGAC	2 cut(s)	273, 1917
BstX2I	RGATCY	2 cut(s)	622, 1718
BstYI	RGATCY	2 cut(s)	622, 1718
BsuRI	GGCC	8 cut(s)	25, 445, 467, 1289, 1662, 1704, 1828, 1918
BtgI	CCRYGG	1 cut(s)	916
BtgZI	GCGATG	1 cut(s)	1258
BtsCI	GGATG	6 cut(s)	325, 446, 491, 1231, 1600, 1945
BtsI	GCAGTG	3 cut(s)	74, 468, 556
BveI	ACCTGC	1 cut(s)	2163
Cac8I	GCNNGC	3 cut(s)	469, 923, 1515
CaiI	CAGNNNCTG	1 cut(s)	982
CciI	TCATGA	1 cut(s)	690
CfoI	GCGC	2 cut(s)	241, 1682
Cfr10I	RCCGGY	1 cut(s)	467
Cfr13I	GGNCC	2 cut(s)	1288, 2009
CsiI	ACCWGGT	1 cut(s)	738
Csp6I	GTAC	5 cut(s)	556, 751, 1009, 1408, 1579
CviQI	GTAC	5 cut(s)	556, 751, 1009, 1408, 1579
DdeI	CTNAG	6 cut(s)	27, 156, 324, 1254, 1404, 1710
DraI	TTTAAA	2 cut(s)	1156, 1222
DraIII	CACNNNGTG	1 cut(s)	1175
DriI	GACNNNNNGTC	1 cut(s)	791
EaeI	YGGCCR	4 cut(s)	465, 1660, 1702, 1826
Eam1104I	CTCTTC	3 cut(s)	1184, 1383, 1584
Eam1105I	GACNNNNNGTC	1 cut(s)	791
EarI	CTCTTC	3 cut(s)	1184, 1383, 1584
Eco130I	CCWWGG	2 cut(s)	916, 1626
Eco24I	GRGCYC	1 cut(s)	565
Eco32I	GATATC	1 cut(s)	886
Eco47I	GGWCC	1 cut(s)	2009
Eco57I	CTGAAG	7 cut(s)	231, 825, 1334, 1341, 1608, 1755, 2138
EcoNI	CCTNNNNNAGG	1 cut(s)	1295
EcoRII	CCWGG	4 cut(s)	738, 787, 1117, 2023
EcoRV	GATATC	1 cut(s)	886
EcoT14I	CCWWGG	2 cut(s)	916, 1626
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	1 cut(s)	565
ErhI	CCWWGG	2 cut(s)	916, 1626
Esp3I	CGTCTC	1 cut(s)	1437
FaqI	GGGAC	1 cut(s)	105
FbaI	TGATCA	1 cut(s)	676
FokI	GGATG	6 cut(s)	332, 453, 498, 1218, 1607, 1952
FriOI	GRGCYC	1 cut(s)	565
FspBI	CTAG	6 cut(s)	390, 947, 1307, 1455, 1473, 2096
GlaI	GCGC	2 cut(s)	240, 1681
GsaI	CCCAGC	1 cut(s)	1104
GsuI	CTGGAG	2 cut(s)	810, 2046
HaeIII	GGCC	8 cut(s)	25, 445, 467, 1289, 1662, 1704, 1828, 1918
HapII	CCGG	4 cut(s)	468, 626, 991, 1993
HhaI	GCGC	2 cut(s)	241, 1682
Hin6I	GCGC	2 cut(s)	239, 1680
HinP1I	GCGC	2 cut(s)	239, 1680
HincII	GTYRAC	2 cut(s)	282, 456
HindII	GTYRAC	2 cut(s)	282, 456
HindIII	AAGCTT	1 cut(s)	695
HpaII	CCGG	4 cut(s)	468, 626, 991, 1993
HphI	GGTGA	2 cut(s)	671, 1161
Hpy166II	GTNNAC	7 cut(s)	282, 456, 556, 801, 845, 1672, 1787
Hpy188I	TCNGA	8 cut(s)	157, 211, 298, 708, 984, 1353, 1442, 1522
Hpy188III	TCNNGA	7 cut(s)	572, 626, 691, 1586, 1788, 2007, 2096
Hpy8I	GTNNAC	7 cut(s)	282, 456, 556, 801, 845, 1672, 1787
Hpy99I	CGWCG	1 cut(s)	1753
HpyCH4III	ACNGT	5 cut(s)	207, 863, 881, 1979, 2126
HpyCH4IV	ACGT	1 cut(s)	687
HpyF3I	CTNAG	6 cut(s)	27, 156, 324, 1254, 1404, 1710
HpySE526I	ACGT	1 cut(s)	687
HspAI	GCGC	2 cut(s)	239, 1680
Kpn2I	TCCGGA	1 cut(s)	625
KroI	GCCGGC	1 cut(s)	467
KroNI	GCCGGC	1 cut(s)	469
Ksp22I	TGATCA	1 cut(s)	676
LmnI	GCTCC	7 cut(s)	422, 757, 1135, 1443, 1631, 1736, 1834
Lsp1109I	GCAGC	8 cut(s)	101, 202, 741, 791, 988, 1481, 1646, 1751
LweI	GCATC	9 cut(s)	220, 310, 1420, 1513, 1526, 1575, 1646, 2007, 2034
MabI	ACCWGGT	1 cut(s)	738
MaeI	CTAG	6 cut(s)	390, 947, 1307, 1455, 1473, 2096
MaeII	ACGT	1 cut(s)	687
MaeIII	GTNAC	8 cut(s)	403, 821, 857, 997, 1896, 1973, 1979, 2126
MflI	RGATCY	2 cut(s)	622, 1718
MhlI	GDGCHC	3 cut(s)	427, 565, 1674
MlsI	TGGCCA	3 cut(s)	1662, 1704, 1828
MluCI	AATT	7 cut(s)	51, 60, 518, 654, 1091, 1199, 1762
MluNI	TGGCCA	3 cut(s)	1662, 1704, 1828
MlyI	GAGTC	4 cut(s)	515, 801, 1493, 1883
MmeI	TCCRAC	1 cut(s)	59
Mox20I	TGGCCA	3 cut(s)	1662, 1704, 1828
Mph1103I	ATGCAT	1 cut(s)	6
MroI	TCCGGA	1 cut(s)	625
MroNI	GCCGGC	1 cut(s)	467
MroXI	GAANNNNTTC	2 cut(s)	1336, 1783
MscI	TGGCCA	3 cut(s)	1662, 1704, 1828
MseI	TTAA	3 cut(s)	816, 1155, 1221
MslI	CAYNNNNRTG	1 cut(s)	864
Msp20I	TGGCCA	3 cut(s)	1662, 1704, 1828
MspI	CCGG	4 cut(s)	468, 626, 991, 1993
MspR9I	CCNGG	5 cut(s)	740, 789, 992, 1119, 2025
Mva1269I	GAATGC	1 cut(s)	1849
MvaI	CCWGG	4 cut(s)	740, 789, 1119, 2025
MvnI	CGCG	2 cut(s)	22, 241
NaeI	GCCGGC	1 cut(s)	469
NciI	CCSGG	1 cut(s)	992
NcoI	CCATGG	1 cut(s)	916
NgoMIV	GCCGGC	1 cut(s)	467
NlaIV	GGNNCC	2 cut(s)	492, 624
NmuCI	GTSAC	3 cut(s)	857, 1896, 2126
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	5 cut(s)	829, 1211, 1729, 2078, 2162
NspV	TTCGAA	1 cut(s)	1777
PagI	TCATGA	1 cut(s)	690
PaqCI	CACCTGC	1 cut(s)	2163
PciI	ACATGT	1 cut(s)	1725
PctI	GAATGC	1 cut(s)	1849
PdiI	GCCGGC	1 cut(s)	469
PdmI	GAANNNNTTC	2 cut(s)	1336, 1783
PfeI	GAWTC	6 cut(s)	160, 575, 1688, 1816, 1996, 2144
PflFI	GACNNNGTC	1 cut(s)	966
PflMI	CCANNNNNTGG	1 cut(s)	1909
PleI	GAGTC	4 cut(s)	514, 800, 1493, 1882
PpsI	GAGTC	4 cut(s)	514, 800, 1493, 1882
PscI	ACATGT	1 cut(s)	1725
Psp6I	CCWGG	4 cut(s)	738, 787, 1117, 2023
PspFI	CCCAGC	1 cut(s)	1100
PspGI	CCWGG	4 cut(s)	738, 787, 1117, 2023
PspN4I	GGNNCC	2 cut(s)	492, 624
PspPI	GGNCC	2 cut(s)	1288, 2009
PstI	CTGCAG	1 cut(s)	220
PstNI	CAGNNNCTG	1 cut(s)	982
PsuI	RGATCY	2 cut(s)	622, 1718
PsyI	GACNNNGTC	1 cut(s)	966
RsaI	GTAC	5 cut(s)	557, 752, 1010, 1409, 1580
RsaNI	GTAC	5 cut(s)	556, 751, 1009, 1408, 1579
RseI	CAYNNNNRTG	1 cut(s)	864
SaqAI	TTAA	3 cut(s)	816, 1155, 1221
Sau96I	GGNCC	2 cut(s)	1288, 2009
SchI	GAGTC	4 cut(s)	515, 801, 1493, 1883
ScrFI	CCNGG	5 cut(s)	740, 789, 992, 1119, 2025
SduI	GDGCHC	3 cut(s)	427, 565, 1674
SexAI	ACCWGGT	1 cut(s)	738
SfaNI	GCATC	9 cut(s)	220, 310, 1420, 1513, 1526, 1575, 1646, 2007, 2034
SfcI	CTRYAG	3 cut(s)	216, 1038, 1311
SfuI	TTCGAA	1 cut(s)	1777
SinI	GGWCC	1 cut(s)	2009
SmiMI	CAYNNNNRTG	1 cut(s)	864
SmlI	CTYRAG	3 cut(s)	497, 848, 2036
SmoI	CTYRAG	3 cut(s)	497, 848, 2036
Sse9I	AATT	7 cut(s)	51, 60, 518, 654, 1091, 1199, 1762
SsiI	CCGC	3 cut(s)	22, 241, 2083
SspMI	CTAG	6 cut(s)	390, 947, 1307, 1455, 1473, 2096
StyD4I	CCNGG	5 cut(s)	738, 787, 990, 1117, 2023
StyI	CCWWGG	2 cut(s)	916, 1626
TaaI	ACNGT	5 cut(s)	207, 863, 881, 1979, 2126
TaiI	ACGT	1 cut(s)	690
TaqI	TCGA	5 cut(s)	716, 1276, 1617, 1751, 1777
TasI	AATT	7 cut(s)	51, 60, 518, 654, 1091, 1199, 1762
TatI	WGTACW	3 cut(s)	555, 750, 1578
TauI	GCSGC	1 cut(s)	25
TfiI	GAWTC	6 cut(s)	160, 575, 1688, 1816, 1996, 2144
Tru1I	TTAA	3 cut(s)	816, 1155, 1221
Tru9I	TTAA	3 cut(s)	816, 1155, 1221
TseFI	GTSAC	3 cut(s)	857, 1896, 2126
TseI	GCWGC	9 cut(s)	10, 114, 215, 729, 779, 976, 1469, 1634, 1739
Tsp45I	GTSAC	3 cut(s)	857, 1896, 2126
TspDTI	ATGAA	6 cut(s)	159, 282, 315, 707, 1272, 1956
TspGWI	ACGGA	1 cut(s)	362
Tth111I	GACNNNGTC	1 cut(s)	966
Van91I	CCANNNNNTGG	1 cut(s)	1909
VneI	GTGCAC	1 cut(s)	1670
VpaK11BI	GGWCC	1 cut(s)	2009
XagI	CCTNNNNNAGG	1 cut(s)	1295
XapI	RAATTY	2 cut(s)	60, 654
XbaI	TCTAGA	1 cut(s)	2095
XceI	RCATGY	5 cut(s)	829, 1211, 1729, 2078, 2162
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1712
XmnI	GAANNNNTTC	2 cut(s)	1336, 1783
XspI	CTAG	6 cut(s)	390, 947, 1307, 1455, 1473, 2096
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.