Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0005566

Physical Location & Seq

Genomic Coordinates Reverse (-)

30118755 .. 30125287

Gene Structure 6533 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002229

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2286 bp

ATGGGATGCATTTGCTCGAAAGGGCTATTAGCCAATCAATATGTTTTGAAGAATCATCGTAGGGACAAAGAATTAAAATCAAATAAGTTGCCAAAGGAATTAGATGGAGCAGCCGTCGGGGTACATGGTGGTGGAAATGATGCAACTACACGGTTGATAACCAATCATCGCACCGAGGACAATGCAGGGTCGACTCCCATTTCATCAGATGAAGGGGAGAAGAAGGTCGGGGTTACCACAGCAGGGGCAAGTGCTCCGAAATTCCCATCTGGCCTAAATAGGATAACTAGCATAACACATGGAGAAAGAGGAGCACAAGTTGTTGCTGGATGGCCTTCTTGGCTCACTGCAGTGGCAGGAGAAGCAATCAGTGGGTGGGTGCCTCGCAAGGCTGATTCATTTGAAAAACTGGACAAGATTGGACAAGGAACTTACAGCAGTGTGTACAGAGCCCGTGATCTTGAATCAAACAAAATAGTTGCATTAAAGAAGGTTCGATTTGCCAATATGGATCCAGAAAGTGTACGTTTTATGTCGAGAGAAATTCTTATTTTGCGCAGGCTTGATCACCCCAATATCATGAAGCTTGAAGGTCTGATTACATCAAGGGTTTCGGGCAGCTTATACCTTGTATTTGAATATATGGAGCATGATCTTGCAGGGATTGCATCAAGACCTGGGATCAAGTTCACTGAAGCACAGATCAAATGTTACATGCAACAGCTTCTTCGTGGACTTGAACATTGTCACAGTCATGGTATTTTGCACCGTGACATCAAGGGATCAAATCTTTTGATCGACAATTATGGCAACCTAAAGATCGCTGACTTTGGTCTAGCAACCTTTTACCGACCTCGTCAAAAGCAGCCTCTAACTAGTCGTGTAGTAACCCTGTGGTACCGACCACCTGAACTTTTGCTTGGCTCTACAGACTATGGGGTTTCTGTGGATTTGTGGAGTTCTGGTTGCATTCTTGCAGAATTGTTTCATGGGAAGCCGATCATGCCTGGAAGAACAGAGGTGGAGCAACTACATAAGATATTTAAGCTCTGTGGGTCACCTTCTGAGGAATACTGGAAGAGATCAAAATTGCCACATGCAACGATTTTTAAACCAACACAACCATACAAGCGTTGTGTTGCAGAGACCTTCAAAGATTTTCCGCACTCAGCAATGGCCCTGTTGGAGGTTCTTCTGGCAGTAGAACCTGACCGTCGTGGAACAGCCTCTTCTGCACTTCAGAGTGAGTTCTTCACATCTATGCCACTTCCCTGTGATCCTTCAACTTTGCCCAAGTACCCACCGAGCAAAGAGTTCGATGCCAAGCTCCGAGATGAGGAAGCTAGAAGGCGAAGAGCATCTGGTGGTAAAGGACATGGAGGGAGTTCCAAAGAATCAAAGGCTGTGCCAGCACCAGTTGGCACTGCTGGGCAGGAATCAACAGAGAGCCAACAAGGGCAACATAAGCCAAAAAGCATCAGCGAGAAGTACATTCCTGAAGAGGATAGTGGCTCTGGCTTTCCTCTTGAACCTCCCAAAGGAGCCACATCAAATGGCTTATCCCATTCAGGCCATTCAATGCACCCAAGTGCATATGGATCTACACGGAATATGGAGATGCGAGGCCAGTCCAAGAATGGCGCAGAGTTGAGAAGGCAGAACTCATATATGAATGGTGGAGCAGCCCAATCTTCCAGATTTTCCAGTTCAGCTTCAGTTCGAAGTGATTCGCGATTTGGTGGTGTTGTAGAAACTACTGCAAATCCACACTGGCCAGAGGAGCGTATTAACACCAGATATAATCATTTAGATGACGGGAATTCCTCTGCAAAACACGATTGGTCCCTTCAATTTCTGAATAGGCCAAAGTCTTCACAGAGAAAGGATGAACAGCCATCTGGAAAGGATTCAGCAAGGGGTTATGCTACCAAGAAGAACCGGATCCACTACTCTGGACCGCTGATGCCCCCTGGGGGAAACCTTGAGGAGATGCTCAAAGAGCATGAAAAACAAATACAACATGCTGTCCGCAAAGCTAGACTGGACAAAGCCAACACCAAGAAAGGCTATGCAAGAGCTCCTTCGAATGGAAAACGTGCTCATGTTAAAGAGAGAATCATTGGGGAACTTCACAAATCTGGCCACCAAAAGATAAATGGAATATATGGAGCTTCATCAAGTTTCATTGCGTCGGATGTATATTCTCTTTTGGAAGATGATGAGAAGGTCTCTGAGGTGGTGTTCAGATGCTGGAGACAAGCTTGTGTACAGCTGAAACCTGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

762

Amino Acids

84.17

Weight (kDa)

9.45

Isoelectric Point (pI)

46.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	134 - 418	1.2e-67	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	137 - 347	4.2e-30	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005566)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G57700
fragaria_vesca	FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28120 FvH4_5g28310
malus_domestica	MD08G1244800.v1.1 MD15G1439900.v1.1
prunus_persica	Prupe.1G582200_v2.0.a1 Prupe.1G582200_v2.0.a1 Prupe.1G582200_v2.0.a1 Prupe.1G582200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0220331
rosa_laevigata	RLG00000002229
rosa_multiflora	Rmu_sc0001136.1_g000085
rosa_roxburghii	Rroxscaffold_3G00239520
rosa_rugosa	Rorug07G0192900
rosa_samantha	Rh7AG334200 Rh7BG325100 Rh7CG351900 Rh7DG330900
rosa_wichuraiana	Rw7G028280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	557
Acc65I	GGTACC	1 cut(s)	897
AccB1I	GGYRCC	2 cut(s)	379, 897
AccI	GTMKAC	1 cut(s)	191
AccII	CGCG	1 cut(s)	1732
AciI	CCGC	3 cut(s)	1163, 1958, 2029
AclWI	GGATC	8 cut(s)	506, 519, 689, 790, 1271, 1606, 1936, 1949
AcoI	YGGCCR	2 cut(s)	1772, 2140
AcsI	RAATTY	3 cut(s)	260, 543, 1819
AcuI	CTGAAG	4 cut(s)	714, 1223, 1518, 1698
AfaI	GTAC	7 cut(s)	123, 446, 525, 899, 1298, 1490, 2268
AfiI	CCNNNNNNNGG	6 cut(s)	243, 1186, 1336, 1538, 1973, 2087
AhlI	ACTAGT	1 cut(s)	875
AjnI	CCWGG	3 cut(s)	676, 1006, 1969
AjuI	GAANNNNNNNTTGG	2 cut(s)	903, 935
AleI	CACNNNNGTG	2 cut(s)	350, 1587
Alw21I	GWGCWC	4 cut(s)	256, 316, 2080, 2101
Alw26I	GTCTC	3 cut(s)	1139, 2233, 2248
AlwI	GGATC	8 cut(s)	506, 519, 689, 790, 1271, 1606, 1936, 1949
AlwNI	CAGNNNCTG	1 cut(s)	2250
AoxI	GGCC	8 cut(s)	271, 332, 1176, 1570, 1624, 1772, 1862, 2140
ApeKI	GCWGC	4 cut(s)	110, 618, 865, 1682
ApoI	RAATTY	3 cut(s)	260, 543, 1819
Asp700I	GAANNNNTTC	3 cut(s)	984, 1726, 1906
Asp718I	GGTACC	1 cut(s)	897
AspLEI	GCGC	2 cut(s)	558, 1643
AspS9I	GGNCC	3 cut(s)	1177, 1842, 1955
AsuHPI	GGTGA	2 cut(s)	560, 1050
AsuII	TTCGAA	2 cut(s)	1720, 2084
AvaII	GGWCC	2 cut(s)	1842, 1955
BalI	TGGCCA	2 cut(s)	1774, 2142
BamHI	GGATCC	2 cut(s)	511, 1941
BanI	GGYRCC	2 cut(s)	379, 897
BanII	GRGCYC	2 cut(s)	454, 2080
BbsI	GAAGAC	1 cut(s)	1863
Bbv12I	GWGCWC	4 cut(s)	256, 316, 2080, 2101
BbvI	GCAGC	4 cut(s)	122, 630, 877, 1694
BccI	CCATC	4 cut(s)	98, 274, 324, 1903
BceAI	ACGGC	1 cut(s)	98
BcgI	CGANNNNNNTGC	4 cut(s)	164, 198, 1297, 1331
BciT130I	CCWGG	3 cut(s)	678, 1008, 1971
BclI	TGATCA	1 cut(s)	565
BcoDI	GTCTC	3 cut(s)	1139, 2233, 2248
BcuI	ACTAGT	1 cut(s)	875
BfaI	CTAG	5 cut(s)	288, 836, 876, 1344, 2037
BfmI	CTRYAG	2 cut(s)	348, 927
BglI	GCCNNNNNGGC	1 cut(s)	340
BisI	GCNGC	4 cut(s)	111, 619, 866, 1683
BlsI	GCNGC	4 cut(s)	112, 620, 867, 1684
Bme1390I	CCNGG	3 cut(s)	678, 1008, 1971
Bme18I	GGWCC	2 cut(s)	1842, 1955
BmgT120I	GGNCC	3 cut(s)	1177, 1842, 1955
BmiI	GGNNCC	6 cut(s)	381, 513, 899, 1543, 1844, 1943
BmrFI	CCNGG	3 cut(s)	678, 1008, 1971
BmsI	GCATC	9 cut(s)	130, 677, 1309, 1367, 1485, 1608, 1953, 1980, 2237
BoxI	GACNNNNGTC	1 cut(s)	831
BpiI	GAAGAC	1 cut(s)	1863
BplI	GAGNNNNNCTC	2 cut(s)	2213, 2245
BpmI	CTGGAG	1 cut(s)	2272
Bpu14I	TTCGAA	2 cut(s)	1720, 2084
BpuEI	CTTGAG	1 cut(s)	2003
BsaI	GGTCTC	2 cut(s)	1139, 2233
BsaJI	CCNNGG	4 cut(s)	174, 677, 1969, 1970
BsaWI	WCCGGW	1 cut(s)	1938
BsaXI	ACNNNNNCTCC	6 cut(s)	99, 129, 303, 333, 949, 979
Bsc4I	CCNNNNNNNGG	6 cut(s)	243, 1186, 1336, 1538, 1973, 2087
Bse1I	ACTGG	7 cut(s)	414, 1079, 1415, 1627, 1704, 1775, 2046
Bse3DI	GCAATG	2 cut(s)	1179, 2184
BseBI	CCWGG	3 cut(s)	678, 1008, 1971
BseDI	CCNNGG	4 cut(s)	174, 677, 1969, 1970
BseGI	GGATG	4 cut(s)	11, 335, 1891, 2200
BseLI	CCNNNNNNNGG	6 cut(s)	243, 1186, 1336, 1538, 1973, 2087
BseMI	GCAATG	2 cut(s)	1179, 2184
BseMII	CTCAG	4 cut(s)	1056, 1182, 2223, 2271
BseNI	ACTGG	7 cut(s)	414, 1079, 1415, 1627, 1704, 1775, 2046
BseRI	GAGGAG	3 cut(s)	324, 1793, 2000
BseXI	GCAGC	4 cut(s)	122, 630, 877, 1694
BseYI	CCCAGC	1 cut(s)	1427
BsgI	GTGCAG	1 cut(s)	1218
Bsh1236I	CGCG	1 cut(s)	1732
BshFI	GGCC	8 cut(s)	273, 334, 1178, 1572, 1626, 1774, 1864, 2142
BshNI	GGYRCC	2 cut(s)	379, 897
BsiHKAI	GWGCWC	4 cut(s)	256, 316, 2080, 2101
BsiSI	CCGG	1 cut(s)	1939
BslFI	GGGAC	2 cut(s)	77, 1828
BslI	CCNNNNNNNGG	6 cut(s)	243, 1186, 1336, 1538, 1973, 2087
BsmAI	GTCTC	3 cut(s)	1139, 2233, 2248
BsmFI	GGGAC	2 cut(s)	77, 1828
BsmI	GAATGC	1 cut(s)	969
BsnI	GGCC	8 cut(s)	273, 334, 1178, 1572, 1626, 1774, 1864, 2142
Bso31I	GGTCTC	2 cut(s)	1139, 2233
Bsp119I	TTCGAA	2 cut(s)	1720, 2084
Bsp1286I	GDGCHC	5 cut(s)	256, 316, 454, 2080, 2101
Bsp1407I	TGTACA	2 cut(s)	444, 2266
Bsp68I	TCGCGA	1 cut(s)	1732
BspACI	CCGC	3 cut(s)	1163, 1958, 2029
BspANI	GGCC	8 cut(s)	273, 334, 1178, 1572, 1626, 1774, 1864, 2142
BspCNI	CTCAG	4 cut(s)	1057, 1181, 2224, 2272
BspFNI	CGCG	1 cut(s)	1732
BspHI	TCATGA	1 cut(s)	579
BspLI	GGNNCC	6 cut(s)	381, 513, 899, 1543, 1844, 1943
BspMAI	CTGCAG	1 cut(s)	352
BspPI	GGATC	8 cut(s)	506, 519, 689, 790, 1271, 1606, 1936, 1949
BspQI	GCTCTTC	1 cut(s)	1348
BspT104I	TTCGAA	2 cut(s)	1720, 2084
BspT107I	GGYRCC	2 cut(s)	379, 897
BspTNI	GGTCTC	2 cut(s)	1139, 2233
BsrDI	GCAATG	2 cut(s)	1179, 2184
BsrGI	TGTACA	2 cut(s)	444, 2266
BsrI	ACTGG	7 cut(s)	414, 1079, 1415, 1627, 1704, 1775, 2046
BssECI	CCNNGG	4 cut(s)	174, 677, 1969, 1970
Bst2UI	CCWGG	3 cut(s)	678, 1008, 1971
Bst4CI	ACNGT	4 cut(s)	153, 752, 770, 1214
Bst6I	CTCTTC	4 cut(s)	1073, 1234, 1348, 1494
BstAPI	GCANNNNNTGC	1 cut(s)	665
BstAUI	TGTACA	2 cut(s)	444, 2266
BstBI	TTCGAA	2 cut(s)	1720, 2084
BstC8I	GCNNGC	2 cut(s)	560, 1410
BstDEI	CTNAG	4 cut(s)	1065, 1168, 2232, 2280
BstEII	GGTNACC	2 cut(s)	232, 1056
BstENI	CCTNNNNNAGG	2 cut(s)	1184, 1536
BstF5I	GGATG	4 cut(s)	11, 335, 1891, 2200
BstFNI	CGCG	1 cut(s)	1732
BstHHI	GCGC	2 cut(s)	558, 1643
BstMAI	GTCTC	3 cut(s)	1139, 2233, 2248
BstMWI	GCNNNNNNNGC	9 cut(s)	340, 362, 665, 1003, 1232, 1409, 1465, 1780, 1999
BstNI	CCWGG	3 cut(s)	678, 1008, 1971
BstNSI	RCATGY	3 cut(s)	718, 1100, 2024
BstPAI	GACNNNNGTC	1 cut(s)	831
BstPI	GGTNACC	2 cut(s)	232, 1056
BstSCI	CCNGG	3 cut(s)	676, 1006, 1969
BstSFI	CTRYAG	2 cut(s)	348, 927
BstUI	CGCG	1 cut(s)	1732
BstV1I	GCAGC	4 cut(s)	122, 630, 877, 1694
BstV2I	GAAGAC	1 cut(s)	1863
BstX2I	RGATCY	3 cut(s)	511, 1598, 1941
BstXI	CCANNNNNNTGG	1 cut(s)	1952
BstYI	RGATCY	3 cut(s)	511, 1598, 1941
BsuRI	GGCC	8 cut(s)	273, 334, 1178, 1572, 1626, 1774, 1864, 2142
BtgZI	GCGATG	1 cut(s)	152
BtsCI	GGATG	4 cut(s)	11, 335, 1891, 2200
BtsI	GCAGTG	4 cut(s)	345, 357, 445, 1422
BtsIMutI	CAGTG	7 cut(s)	345, 357, 376, 445, 690, 1422, 1768
BtuMI	TCGCGA	1 cut(s)	1732
Cac8I	GCNNGC	2 cut(s)	560, 1410
CaiI	CAGNNNCTG	1 cut(s)	2250
CciI	TCATGA	1 cut(s)	579
CfoI	GCGC	2 cut(s)	558, 1643
Cfr13I	GGNCC	3 cut(s)	1177, 1842, 1955
CseI	GACGC	1 cut(s)	2178
Csp6I	GTAC	7 cut(s)	122, 445, 524, 898, 1297, 1489, 2267
CviQI	GTAC	7 cut(s)	122, 445, 524, 898, 1297, 1489, 2267
DdeI	CTNAG	4 cut(s)	1065, 1168, 2232, 2280
DraI	TTTAAA	1 cut(s)	1111
EaeI	YGGCCR	2 cut(s)	1772, 2140
Eam1104I	CTCTTC	4 cut(s)	1073, 1234, 1348, 1494
EarI	CTCTTC	4 cut(s)	1073, 1234, 1348, 1494
Ecl136II	GAGCTC	1 cut(s)	2078
Eco24I	GRGCYC	2 cut(s)	454, 2080
Eco31I	GGTCTC	2 cut(s)	1139, 2233
Eco47I	GGWCC	2 cut(s)	1842, 1955
Eco53kI	GAGCTC	1 cut(s)	2078
Eco57I	CTGAAG	4 cut(s)	714, 1223, 1518, 1698
Eco91I	GGTNACC	2 cut(s)	232, 1056
EcoICRI	GAGCTC	1 cut(s)	2078
EcoNI	CCTNNNNNAGG	2 cut(s)	1184, 1536
EcoO65I	GGTNACC	2 cut(s)	232, 1056
EcoRI	GAATTC	1 cut(s)	1819
EcoRII	CCWGG	3 cut(s)	676, 1006, 1969
EcoT22I	ATGCAT	1 cut(s)	11
EcoT38I	GRGCYC	2 cut(s)	454, 2080
FalI	AAGNNNNNCTT	2 cut(s)	2065, 2097
FaqI	GGGAC	2 cut(s)	77, 1828
FauNDI	CATATG	1 cut(s)	1594
FbaI	TGATCA	1 cut(s)	565
FblI	GTMKAC	1 cut(s)	191
Fnu4HI	GCNGC	4 cut(s)	111, 619, 866, 1683
FokI	GGATG	4 cut(s)	18, 342, 1898, 2207
FriOI	GRGCYC	2 cut(s)	454, 2080
Fsp4HI	GCNGC	4 cut(s)	111, 619, 866, 1683
FspBI	CTAG	5 cut(s)	288, 836, 876, 1344, 2037
FspI	TGCGCA	1 cut(s)	557
GlaI	GCGC	2 cut(s)	557, 1642
GluI	GCNGC	4 cut(s)	111, 619, 866, 1683
GsaI	CCCAGC	1 cut(s)	1431
GsuI	CTGGAG	1 cut(s)	2272
HaeIII	GGCC	8 cut(s)	273, 334, 1178, 1572, 1626, 1774, 1864, 2142
HapII	CCGG	1 cut(s)	1939
HgaI	GACGC	1 cut(s)	2178
HhaI	GCGC	2 cut(s)	558, 1643
Hin6I	GCGC	2 cut(s)	556, 1641
HinP1I	GCGC	2 cut(s)	556, 1641
HincII	GTYRAC	1 cut(s)	192
HindII	GTYRAC	1 cut(s)	192
HindIII	AAGCTT	2 cut(s)	584, 2259
HinfI	GANTC	9 cut(s)	52, 193, 395, 464, 1394, 1436, 1727, 1907, 2115
HpaII	CCGG	1 cut(s)	1939
HphI	GGTGA	2 cut(s)	560, 1050
Hpy166II	GTNNAC	6 cut(s)	192, 445, 524, 690, 734, 2267
Hpy8I	GTNNAC	6 cut(s)	192, 445, 524, 690, 734, 2267
Hpy99I	CGWCG	3 cut(s)	119, 1218, 2194
HpyCH4III	ACNGT	4 cut(s)	153, 752, 770, 1214
HpyCH4IV	ACGT	2 cut(s)	526, 2095
HpyF10VI	GCNNNNNNNGC	9 cut(s)	340, 362, 665, 1003, 1232, 1409, 1465, 1780, 1999
HpyF3I	CTNAG	4 cut(s)	1065, 1168, 2232, 2280
HpySE526I	ACGT	2 cut(s)	526, 2095
HspAI	GCGC	2 cut(s)	556, 1641
KpnI	GGTACC	1 cut(s)	901
Ksp22I	TGATCA	1 cut(s)	565
LguI	GCTCTTC	1 cut(s)	1348
Lsp1109I	GCAGC	4 cut(s)	122, 630, 877, 1694
LweI	GCATC	9 cut(s)	130, 677, 1309, 1367, 1485, 1608, 1953, 1980, 2237
MaeI	CTAG	5 cut(s)	288, 836, 876, 1344, 2037
MaeII	ACGT	2 cut(s)	526, 2095
MaeIII	GTNAC	6 cut(s)	232, 710, 746, 770, 886, 1056
MflI	RGATCY	3 cut(s)	511, 1598, 1941
MhlI	GDGCHC	5 cut(s)	256, 316, 454, 2080, 2101
MlsI	TGGCCA	2 cut(s)	1774, 2142
MluCI	AATT	9 cut(s)	71, 98, 260, 543, 802, 980, 1088, 1819, 1850
MluNI	TGGCCA	2 cut(s)	1774, 2142
MlyI	GAGTC	1 cut(s)	187
MmeI	TCCRAC	2 cut(s)	1164, 2172
Mox20I	TGGCCA	2 cut(s)	1774, 2142
Mph1103I	ATGCAT	1 cut(s)	11
MroXI	GAANNNNTTC	3 cut(s)	984, 1726, 1906
MscI	TGGCCA	2 cut(s)	1774, 2142
MseI	TTAA	6 cut(s)	74, 485, 1044, 1110, 1788, 2106
MslI	CAYNNNNRTG	6 cut(s)	129, 350, 753, 1259, 1587, 1809
Msp20I	TGGCCA	2 cut(s)	1774, 2142
MspA1I	CMGCKG	2 cut(s)	1960, 2272
MspI	CCGG	1 cut(s)	1939
MspR9I	CCNGG	3 cut(s)	678, 1008, 1971
Mva1269I	GAATGC	1 cut(s)	969
MvaI	CCWGG	3 cut(s)	678, 1008, 1971
MvnI	CGCG	1 cut(s)	1732
MwoI	GCNNNNNNNGC	9 cut(s)	340, 362, 665, 1003, 1232, 1409, 1465, 1780, 1999
NdeI	CATATG	1 cut(s)	1594
NlaIV	GGNNCC	6 cut(s)	381, 513, 899, 1543, 1844, 1943
NmuCI	GTSAC	3 cut(s)	746, 770, 1056
NruI	TCGCGA	1 cut(s)	1732
NsbI	TGCGCA	1 cut(s)	557
NsiI	ATGCAT	1 cut(s)	11
NspI	RCATGY	3 cut(s)	718, 1100, 2024
NspV	TTCGAA	2 cut(s)	1720, 2084
OliI	CACNNNNGTG	2 cut(s)	350, 1587
PagI	TCATGA	1 cut(s)	579
PasI	CCCWGGG	1 cut(s)	1970
PciSI	GCTCTTC	1 cut(s)	1348
PctI	GAATGC	1 cut(s)	969
PdmI	GAANNNNTTC	3 cut(s)	984, 1726, 1906
PfeI	GAWTC	8 cut(s)	52, 395, 464, 1394, 1436, 1727, 1907, 2115
PflFI	GACNNNGTC	1 cut(s)	855
PkrI	GCNGC	4 cut(s)	112, 620, 867, 1684
PleI	GAGTC	1 cut(s)	187
PpsI	GAGTC	1 cut(s)	187
PshAI	GACNNNNGTC	1 cut(s)	831
Psp124BI	GAGCTC	1 cut(s)	2080
Psp6I	CCWGG	3 cut(s)	676, 1006, 1969
PspEI	GGTNACC	2 cut(s)	232, 1056
PspFI	CCCAGC	1 cut(s)	1427
PspGI	CCWGG	3 cut(s)	676, 1006, 1969
PspN4I	GGNNCC	6 cut(s)	381, 513, 899, 1543, 1844, 1943
PspPI	GGNCC	3 cut(s)	1177, 1842, 1955
PstI	CTGCAG	1 cut(s)	352
PstNI	CAGNNNCTG	1 cut(s)	2250
PsuI	RGATCY	3 cut(s)	511, 1598, 1941
PsyI	GACNNNGTC	1 cut(s)	855
PvuII	CAGCTG	1 cut(s)	2272
RruI	TCGCGA	1 cut(s)	1732
RsaI	GTAC	7 cut(s)	123, 446, 525, 899, 1298, 1490, 2268
RsaNI	GTAC	7 cut(s)	122, 445, 524, 898, 1297, 1489, 2267
RseI	CAYNNNNRTG	6 cut(s)	129, 350, 753, 1259, 1587, 1809
SacI	GAGCTC	1 cut(s)	2080
SalI	GTCGAC	1 cut(s)	190
SapI	GCTCTTC	1 cut(s)	1348
SaqAI	TTAA	6 cut(s)	74, 485, 1044, 1110, 1788, 2106
SatI	GCNGC	4 cut(s)	111, 619, 866, 1683
Sau96I	GGNCC	3 cut(s)	1177, 1842, 1955
SchI	GAGTC	1 cut(s)	187
ScrFI	CCNGG	3 cut(s)	678, 1008, 1971
SduI	GDGCHC	5 cut(s)	256, 316, 454, 2080, 2101
SfaNI	GCATC	9 cut(s)	130, 677, 1309, 1367, 1485, 1608, 1953, 1980, 2237
SfcI	CTRYAG	2 cut(s)	348, 927
SfuI	TTCGAA	2 cut(s)	1720, 2084
SinI	GGWCC	2 cut(s)	1842, 1955
SmiMI	CAYNNNNRTG	6 cut(s)	129, 350, 753, 1259, 1587, 1809
SmlI	CTYRAG	1 cut(s)	1982
SmoI	CTYRAG	1 cut(s)	1982
SpeI	ACTAGT	1 cut(s)	875
Sse9I	AATT	9 cut(s)	71, 98, 260, 543, 802, 980, 1088, 1819, 1850
SsiI	CCGC	3 cut(s)	1163, 1958, 2029
SspMI	CTAG	5 cut(s)	288, 836, 876, 1344, 2037
SstI	GAGCTC	1 cut(s)	2080
StyD4I	CCNGG	3 cut(s)	676, 1006, 1969
TaaI	ACNGT	4 cut(s)	153, 752, 770, 1214
TaiI	ACGT	2 cut(s)	529, 2098
TaqI	TCGA	8 cut(s)	17, 191, 496, 536, 798, 1317, 1720, 2084
TasI	AATT	9 cut(s)	71, 98, 260, 543, 802, 980, 1088, 1819, 1850
TatI	WGTACW	3 cut(s)	444, 1488, 2266
TfiI	GAWTC	8 cut(s)	52, 395, 464, 1394, 1436, 1727, 1907, 2115
Tru1I	TTAA	6 cut(s)	74, 485, 1044, 1110, 1788, 2106
Tru9I	TTAA	6 cut(s)	74, 485, 1044, 1110, 1788, 2106
TscAI	CASTG	7 cut(s)	352, 357, 376, 445, 697, 1429, 1775
TseFI	GTSAC	3 cut(s)	746, 770, 1056
TseI	GCWGC	4 cut(s)	110, 618, 865, 1682
Tsp45I	GTSAC	3 cut(s)	746, 770, 1056
TspGWI	ACGGA	1 cut(s)	1621
TspRI	CASTG	7 cut(s)	352, 357, 376, 445, 697, 1429, 1775
Tth111I	GACNNNGTC	1 cut(s)	855
VpaK11BI	GGWCC	2 cut(s)	1842, 1955
XagI	CCTNNNNNAGG	2 cut(s)	1184, 1536
XapI	RAATTY	3 cut(s)	260, 543, 1819
XceI	RCATGY	3 cut(s)	718, 1100, 2024
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1634, 2153
XmiI	GTMKAC	1 cut(s)	191
XmnI	GAANNNNTTC	3 cut(s)	984, 1726, 1906
XspI	CTAG	5 cut(s)	288, 836, 876, 1344, 2037
Zsp2I	ATGCAT	1 cut(s)	11

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.