Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0012222

Physical Location & Seq

Genomic Coordinates Reverse (-)

1720937 .. 1724816

Gene Structure 3880 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1027600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1941 bp

ATGGTGAAGCACCCCGCCGCCCCGGTGAAGCATAAGCATTACAGGCCTGCGGGAAAGAAGAAGGAAGGAAATGCCGCGAGGTACGTCACCAGGTCACAAGCTGTCAAGCAGCTTCAAGTCAGTCTTCCCCTCTTCAGGAAATTGTGTATTCTAAAGGGCGTGTTCCCTCGGGAGCCGAAGAAGAAGGTGAAAGGAAATCACCATACCTATTATCACTTGAAGGATGTTTCTTTCATTCAGCATGAACCGCTACTCGAGCGGTTGAGAGAGATAAGAGCATACGAAAGAAAAGTAAAGAAAGCTGATGCGAAGAAGAACAAGGAACGGGCTGCTCTCCTGAAAGAGAGAAGACCCACTTACAAATTAGATAGAATTATTTTGCAGAGGTATCCAAAATTTGTTGACGCCCTCAGAGATATAGACGACTGCATCACAATGGTTCACCTCTTTGCGGCATTACCTGCGATTGAGGACAGAATTGATGTCAAACGCATTCATAACTGTCGAAGGTTGAGTCATGAATGGCAAGCTTACATATCTCGGACTCATAAATTGCGTAAAGTCTTTGTATCTGTTAAAGGCATATATTATCAGGCCGAGGTTAAGGGGCAAAAAGTCACATGGTTGACCCCTCACCCTTTGCAGCAAGTTTTGACTGATGATATTGACTTCAATATCATGCTAAACTTTTTGGAATTTTACGAGGCTCTTCTTGCATTTGTGAATTGTCATTTATATCGTTCCATAGATGTGAAGTACCCACCGATTCTTGATTCTCGATTGGAAGCTTTAGCAGCAGAACTTTATGCACTAGCACGATACCTTGATGCAAATTCTCGGTCTGCAGTGTTGGATTCTCAAGCTTCTAGTTTGTCTGGATCTGGGAATGGTGAGAACAAGAAGATTGAGACTTCACTTGAAGAGTCTGAACTAAGACTTGCTCAACTTCAGCATCAACTGCCTTCGAATGAACCAGGTGCATTGATGCACCTTGTTAAAGATGTTTCTGGCGAGGATGAAGAGGATAATGATACAAAGGAGTGTAAGCAACTCTTCAAGGGTATGAAATTTTTCTTGAACCGTGAGGTTTACAGAGAGTCGTTGCTTTTTGTCATTCCTGCTTTTGGTGGCACTGTTTCCTGGGAAGGTGATGGGGCTCCATTTGAGGAAGCTGATGACAGCATTACCCATCAGATTGTTGATAGGCCAATGCATGGTCGTCAGACCCTTGCCAGGGAATATGTTCAACCACAATGGGTTTATGACTGTATAAATGCTCGGATCATTTTGCCAACTGGGGGTTATTTGGCGGGCAGGGATCCTCCACCACACTTGTCACCATTTGTTGACAACGAGGCAGAAGGTTATGTTCCGGATTATGCTGAGACCATAAAAAAATGGAAGTCTGCTGGTGAAGTCCTCCCCCTGCCAGGTGTGGGACAAGAAGATTTGGAAGATCCTCAGAATTTACTGTCTGAAGGTGTTATTGACCGAGCAAAAGCAAAAGAAGCTGCAGAGAGACAGAAGAAGATGATGGCTCTTGAAAAGCAGTATCACGACGAATTGAATATGGAGCTTCAGGGCATCCAACATTCATCATCTACCTCGAATGTAGATAATCAGAACTCTGCCAAGGAAACCACAGAGGATATGGAAGAGTTTGATCCTGATACTGATCGTGACAATATGTCTAAAATGTTAATGTCCCGTAAGAAGAGGGGTGTTCTTGAAGCTATGGAGGCAAAAAGCGAAAGAAGGACCACGTTGATGTCATCAGGGAACGGAAGAGAAAACTTAAAGAATCTAACAAATAAGCGGGCTAAGGCAGTCGAGAAAACTGCGGTGTTGTTATTGACATGCTGTTTTCGGAGCTCAATGGTCTGGAGAGTGTTGATGCCACCGTGGAAATTTTGGTGTTTGATTTTGCTAATGACCGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000463 GO:0000470 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0010468 GO:0016070 GO:0016072 GO:0019219 GO:0019222 GO:0022613 GO:0030684 GO:0030686 GO:0031323 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042273 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044087 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0070545 GO:0071704 GO:0071840 GO:0080090 GO:0090069 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904 GO:2000232

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRCT_2 Pescadillo_N

Protein Analysis

647

Amino Acids

74.22

Weight (kDa)

8.73

Isoelectric Point (pI)

47.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pescadillo_N	PF06732	19 - 281	7e-110	Pescadillo N-terminus
BRCT_2	PF16589	350 - 433	8.4e-06	BRCT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14520
fragaria_vesca	FvH4_3g26080 FvH4_3g26080 FvH4_3g26080
malus_domestica	MD09G1027600.v1.1 MD17G1027600.v1.1
prunus_persica	Prupe.3G291100_v2.0.a1
pyrus_communis	pycom111g02170 pycom17g02260
rosa_chinensis	RchiOBHm_Chr5g0047391
rosa_laevigata	RLG00000034492
rosa_multiflora	Rmu_sc0000580.1_g000062 Rmu_sc0003441.1_g000056
rosa_roxburghii	Rroxscaffold_1G00034010
rosa_rugosa	Rorug05G0236100
rosa_samantha	Rh5AG314900 Rh5CG350800 Rh5DG334700
rosa_wichuraiana	Rw5G029430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	469
AccB7I	CCANNNNNTGG	1 cut(s)	1214
AccBSI	CCGCTC	1 cut(s)	259
AccII	CGCG	1 cut(s)	77
AccIII	TCCGGA	1 cut(s)	1372
AclWI	GGATC	6 cut(s)	886, 1289, 1313, 1326, 1451, 1658
AcsI	RAATTY	6 cut(s)	395, 695, 832, 1067, 1465, 1907
AcuI	CTGAAG	4 cut(s)	118, 932, 1497, 1562
AcyI	GRCGYC	1 cut(s)	405
AfaI	GTAC	2 cut(s)	83, 758
AfiI	CCNNNNNNNGG	9 cut(s)	135, 451, 1124, 1214, 1233, 1234, 1298, 1430, 1436
AhdI	GACNNNNNGTC	1 cut(s)	1687
AjnI	CCWGG	5 cut(s)	89, 973, 1139, 1232, 1429
Alw21I	GWGCWC	1 cut(s)	1874
Alw26I	GTCTC	3 cut(s)	902, 1379, 1513
AlwI	GGATC	6 cut(s)	886, 1289, 1313, 1326, 1451, 1658
Ama87I	CYCGRG	2 cut(s)	168, 254
Aor13HI	TCCGGA	1 cut(s)	1372
AoxI	GGCC	3 cut(s)	44, 594, 1205
ApeKI	GCWGC	5 cut(s)	109, 329, 643, 794, 1511
ApoI	RAATTY	6 cut(s)	395, 695, 832, 1067, 1465, 1907
ArsI	GACNNNNNNTTYG	4 cut(s)	499, 531, 824, 856
Asp700I	GAANNNNTTC	2 cut(s)	1070, 1242
AspS9I	GGNCC	1 cut(s)	1758
AsuC2I	CCSGG	1 cut(s)	23
AsuII	TTCGAA	1 cut(s)	965
AvaI	CYCGRG	2 cut(s)	168, 254
AvaII	GGWCC	1 cut(s)	1758
BamHI	GGATCC	1 cut(s)	1318
BanII	GRGCYC	2 cut(s)	1159, 1874
BbsI	GAAGAC	2 cut(s)	116, 355
Bbv12I	GWGCWC	1 cut(s)	1874
BbvI	GCAGC	5 cut(s)	121, 316, 655, 806, 1498
BccI	CCATC	3 cut(s)	1145, 1197, 1528
BcgI	CGANNNNNNTGC	2 cut(s)	1820, 1854
BciT130I	CCWGG	5 cut(s)	91, 975, 1141, 1234, 1431
BciVI	GTATCC	1 cut(s)	399
BcnI	CCSGG	1 cut(s)	23
BcoDI	GTCTC	3 cut(s)	902, 1379, 1513
BfaI	CTAG	2 cut(s)	812, 867
BfmI	CTRYAG	2 cut(s)	843, 1512
BfuAI	ACCTGC	1 cut(s)	469
BfuI	GTATCC	1 cut(s)	399
BisI	GCNGC	8 cut(s)	18, 75, 110, 330, 453, 644, 795, 1512
BlsI	GCNGC	8 cut(s)	19, 76, 111, 331, 454, 645, 796, 1513
Bme1390I	CCNGG	6 cut(s)	23, 91, 975, 1141, 1234, 1431
Bme18I	GGWCC	1 cut(s)	1758
BmeRI	GACNNNNNGTC	1 cut(s)	1687
BmeT110I	CYCGRG	2 cut(s)	168, 254
BmgT120I	GGNCC	1 cut(s)	1758
BmiI	GGNNCC	3 cut(s)	174, 1158, 1320
BmrFI	CCNGG	6 cut(s)	23, 91, 975, 1141, 1234, 1431
BmrI	ACTGGG	1 cut(s)	1305
BmsI	GCATC	7 cut(s)	295, 438, 817, 961, 975, 1593, 1884
BmuI	ACTGGG	1 cut(s)	1305
BpiI	GAAGAC	2 cut(s)	116, 355
BpmI	CTGGAG	1 cut(s)	1903
Bpu10I	CCTNAGC	1 cut(s)	1821
Bpu14I	TTCGAA	1 cut(s)	965
BpuEI	CTTGAG	1 cut(s)	843
BpuMI	CCSGG	1 cut(s)	23
BsaBI	GATNNNNATC	1 cut(s)	1674
BsaHI	GRCGYC	1 cut(s)	405
BsaI	GGTCTC	1 cut(s)	1379
BsaJI	CCNNGG	7 cut(s)	21, 167, 597, 1140, 1233, 1632, 1901
BsaWI	WCCGGW	1 cut(s)	1372
Bsc4I	CCNNNNNNNGG	9 cut(s)	135, 451, 1124, 1214, 1233, 1234, 1298, 1430, 1436
Bse1I	ACTGG	1 cut(s)	1300
Bse8I	GATNNNNATC	1 cut(s)	1674
BseAI	TCCGGA	1 cut(s)	1372
BseBI	CCWGG	5 cut(s)	91, 975, 1141, 1234, 1431
BseDI	CCNNGG	7 cut(s)	21, 167, 597, 1140, 1233, 1632, 1901
BseGI	GGATG	3 cut(s)	229, 1021, 1584
BseJI	GATNNNNATC	1 cut(s)	1674
BseLI	CCNNNNNNNGG	9 cut(s)	135, 451, 1124, 1214, 1233, 1234, 1298, 1430, 1436
BseMII	CTCAG	3 cut(s)	424, 1374, 1475
BseNI	ACTGG	1 cut(s)	1300
BseXI	GCAGC	5 cut(s)	121, 316, 655, 806, 1498
Bsh1236I	CGCG	1 cut(s)	77
BshFI	GGCC	3 cut(s)	46, 596, 1207
BsiHKAI	GWGCWC	1 cut(s)	1874
BsiHKCI	CYCGRG	2 cut(s)	168, 254
BsiSI	CCGG	2 cut(s)	23, 1373
BslFI	GGGAC	2 cut(s)	1452, 1690
BslI	CCNNNNNNNGG	9 cut(s)	135, 451, 1124, 1214, 1233, 1234, 1298, 1430, 1436
BsmAI	GTCTC	3 cut(s)	902, 1379, 1513
BsmFI	GGGAC	2 cut(s)	1452, 1690
BsmI	GAATGC	1 cut(s)	492
BsnI	GGCC	3 cut(s)	46, 596, 1207
Bso31I	GGTCTC	1 cut(s)	1379
BsoBI	CYCGRG	2 cut(s)	168, 254
Bsp119I	TTCGAA	1 cut(s)	965
Bsp1286I	GDGCHC	2 cut(s)	1159, 1874
Bsp13I	TCCGGA	1 cut(s)	1372
Bsp143I	GATC	6 cut(s)	878, 1281, 1318, 1456, 1663, 1675
BspANI	GGCC	3 cut(s)	46, 596, 1207
BspCNI	CTCAG	3 cut(s)	423, 1375, 1474
BspEI	TCCGGA	1 cut(s)	1372
BspFNI	CGCG	1 cut(s)	77
BspHI	TCATGA	1 cut(s)	517
BspLI	GGNNCC	3 cut(s)	174, 1158, 1320
BspMAI	CTGCAG	2 cut(s)	847, 1516
BspMI	ACCTGC	1 cut(s)	469
BspPI	GGATC	6 cut(s)	886, 1289, 1313, 1326, 1451, 1658
BspQI	GCTCTTC	1 cut(s)	714
BspT104I	TTCGAA	1 cut(s)	965
BspTNI	GGTCTC	1 cut(s)	1379
BsrBI	CCGCTC	1 cut(s)	259
BsrI	ACTGG	1 cut(s)	1300
BssECI	CCNNGG	7 cut(s)	21, 167, 597, 1140, 1233, 1632, 1901
BssMI	GATC	6 cut(s)	878, 1281, 1318, 1456, 1663, 1675
BssNI	GRCGYC	1 cut(s)	405
BssT1I	CCWWGG	1 cut(s)	1632
Bst2UI	CCWGG	5 cut(s)	91, 975, 1141, 1234, 1431
Bst4CI	ACNGT	6 cut(s)	503, 1082, 1135, 1268, 1473, 1902
Bst6I	CTCTTC	8 cut(s)	137, 714, 915, 1014, 1058, 1650, 1709, 1780
BstACI	GRCGYC	1 cut(s)	405
BstAPI	GCANNNNNTGC	2 cut(s)	461, 958
BstBI	TTCGAA	1 cut(s)	965
BstC8I	GCNNGC	4 cut(s)	48, 528, 1312, 1818
BstDEI	CTNAG	5 cut(s)	410, 932, 1383, 1461, 1821
BstDSI	CCRYGG	1 cut(s)	1901
BstF5I	GGATG	3 cut(s)	229, 1021, 1584
BstFNI	CGCG	1 cut(s)	77
BstKTI	GATC	6 cut(s)	881, 1284, 1321, 1459, 1666, 1678
BstMAI	GTCTC	3 cut(s)	902, 1379, 1513
BstMBI	GATC	6 cut(s)	878, 1281, 1318, 1456, 1663, 1675
BstMWI	GCNNNNNNNGC	9 cut(s)	43, 247, 256, 461, 713, 794, 958, 1508, 1739
BstNI	CCWGG	5 cut(s)	91, 975, 1141, 1234, 1431
BstNSI	RCATGY	1 cut(s)	1860
BstSCI	CCNGG	6 cut(s)	21, 89, 973, 1139, 1232, 1429
BstSFI	CTRYAG	2 cut(s)	843, 1512
BstUI	CGCG	1 cut(s)	77
BstV1I	GCAGC	5 cut(s)	121, 316, 655, 806, 1498
BstV2I	GAAGAC	2 cut(s)	116, 355
BstX2I	RGATCY	3 cut(s)	878, 1318, 1456
BstYI	RGATCY	3 cut(s)	878, 1318, 1456
BsuI	GTATCC	1 cut(s)	399
BsuRI	GGCC	3 cut(s)	46, 596, 1207
BtgI	CCRYGG	1 cut(s)	1901
BtsCI	GGATG	3 cut(s)	229, 1021, 1584
BtsI	GCAGTG	1 cut(s)	852
BtsIMutI	CAGTG	2 cut(s)	852, 1131
BveI	ACCTGC	1 cut(s)	469
Cac8I	GCNNGC	4 cut(s)	48, 528, 1312, 1818
CciI	TCATGA	1 cut(s)	517
Cfr13I	GGNCC	1 cut(s)	1758
CseI	GACGC	1 cut(s)	413
CsiI	ACCWGGT	2 cut(s)	89, 973
Csp6I	GTAC	2 cut(s)	82, 757
CspCI	CAANNNNNGTGG	2 cut(s)	1314, 1349
CviAII	CATG	6 cut(s)	242, 518, 621, 679, 1214, 1857
CviQI	GTAC	2 cut(s)	82, 757
DdeI	CTNAG	5 cut(s)	410, 932, 1383, 1461, 1821
DpnI	GATC	6 cut(s)	880, 1283, 1320, 1458, 1665, 1677
DpnII	GATC	6 cut(s)	878, 1281, 1318, 1456, 1663, 1675
DriI	GACNNNNNGTC	1 cut(s)	1687
Eam1104I	CTCTTC	8 cut(s)	137, 714, 915, 1014, 1058, 1650, 1709, 1780
Eam1105I	GACNNNNNGTC	1 cut(s)	1687
EarI	CTCTTC	8 cut(s)	137, 714, 915, 1014, 1058, 1650, 1709, 1780
Ecl136II	GAGCTC	1 cut(s)	1872
Eco130I	CCWWGG	1 cut(s)	1632
Eco147I	AGGCCT	1 cut(s)	46
Eco24I	GRGCYC	2 cut(s)	1159, 1874
Eco31I	GGTCTC	1 cut(s)	1379
Eco47I	GGWCC	1 cut(s)	1758
Eco53kI	GAGCTC	1 cut(s)	1872
Eco57I	CTGAAG	4 cut(s)	118, 932, 1497, 1562
Eco88I	CYCGRG	2 cut(s)	168, 254
EcoICRI	GAGCTC	1 cut(s)	1872
EcoRII	CCWGG	5 cut(s)	89, 973, 1139, 1232, 1429
EcoT14I	CCWWGG	1 cut(s)	1632
EcoT22I	ATGCAT	1 cut(s)	1215
EcoT38I	GRGCYC	2 cut(s)	1159, 1874
ErhI	CCWWGG	1 cut(s)	1632
FaeI	CATG	6 cut(s)	245, 521, 624, 682, 1217, 1860
FalI	AAGNNNNNCTT	6 cut(s)	108, 140, 340, 372, 1037, 1069
FaqI	GGGAC	2 cut(s)	1452, 1690
FatI	CATG	6 cut(s)	241, 517, 620, 678, 1213, 1856
FauI	CCCGC	4 cut(s)	22, 43, 1303, 1809
Fnu4HI	GCNGC	8 cut(s)	18, 75, 110, 330, 453, 644, 795, 1512
FokI	GGATG	3 cut(s)	236, 1028, 1571
FriOI	GRGCYC	2 cut(s)	1159, 1874
Fsp4HI	GCNGC	8 cut(s)	18, 75, 110, 330, 453, 644, 795, 1512
FspBI	CTAG	2 cut(s)	812, 867
GluI	GCNGC	8 cut(s)	18, 75, 110, 330, 453, 644, 795, 1512
GsuI	CTGGAG	1 cut(s)	1903
HaeIII	GGCC	3 cut(s)	46, 596, 1207
HapII	CCGG	2 cut(s)	23, 1373
HgaI	GACGC	1 cut(s)	413
Hin1I	GRCGYC	1 cut(s)	405
Hin1II	CATG	6 cut(s)	245, 521, 624, 682, 1217, 1860
HincII	GTYRAC	3 cut(s)	403, 627, 1348
HindII	GTYRAC	3 cut(s)	403, 627, 1348
HindIII	AAGCTT	3 cut(s)	528, 786, 861
HinfI	GANTC	8 cut(s)	514, 544, 766, 773, 854, 923, 1097, 1801
HpaII	CCGG	2 cut(s)	23, 1373
Hpy166II	GTNNAC	5 cut(s)	403, 442, 627, 1090, 1348
Hpy8I	GTNNAC	5 cut(s)	403, 442, 627, 1090, 1348
Hpy99I	CGWCG	1 cut(s)	1562
HpyCH4III	ACNGT	6 cut(s)	503, 1082, 1135, 1268, 1473, 1902
HpyCH4IV	ACGT	2 cut(s)	84, 1763
HpyF10VI	GCNNNNNNNGC	9 cut(s)	43, 247, 256, 461, 713, 794, 958, 1508, 1739
HpyF3I	CTNAG	5 cut(s)	410, 932, 1383, 1461, 1821
HpySE526I	ACGT	2 cut(s)	84, 1763
Hsp92I	GRCGYC	1 cut(s)	405
Hsp92II	CATG	6 cut(s)	245, 521, 624, 682, 1217, 1860
Kpn2I	TCCGGA	1 cut(s)	1372
Kzo9I	GATC	6 cut(s)	878, 1281, 1318, 1456, 1663, 1675
LguI	GCTCTTC	1 cut(s)	714
LmnI	GCTCC	4 cut(s)	172, 1162, 1573, 1869
Lsp1109I	GCAGC	5 cut(s)	121, 316, 655, 806, 1498
LweI	GCATC	7 cut(s)	295, 438, 817, 961, 975, 1593, 1884
MabI	ACCWGGT	2 cut(s)	89, 973
MaeI	CTAG	2 cut(s)	812, 867
MaeII	ACGT	2 cut(s)	84, 1763
MaeIII	GTNAC	5 cut(s)	85, 93, 616, 1335, 1679
MalI	GATC	6 cut(s)	880, 1283, 1320, 1458, 1665, 1677
MbiI	CCGCTC	1 cut(s)	259
MboI	GATC	6 cut(s)	878, 1281, 1318, 1456, 1663, 1675
MflI	RGATCY	3 cut(s)	878, 1318, 1456
MhlI	GDGCHC	2 cut(s)	1159, 1874
MlyI	GAGTC	4 cut(s)	523, 538, 932, 1106
MmeI	TCCRAC	2 cut(s)	831, 1612
Mph1103I	ATGCAT	1 cut(s)	1215
MroI	TCCGGA	1 cut(s)	1372
MroXI	GAANNNNTTC	2 cut(s)	1070, 1242
MseI	TTAA	5 cut(s)	576, 603, 996, 1700, 1796
MslI	CAYNNNNRTG	3 cut(s)	434, 749, 1766
MspI	CCGG	2 cut(s)	23, 1373
MspR9I	CCNGG	6 cut(s)	23, 91, 975, 1141, 1234, 1431
Mva1269I	GAATGC	1 cut(s)	492
MvaI	CCWGG	5 cut(s)	91, 975, 1141, 1234, 1431
MvnI	CGCG	1 cut(s)	77
MwoI	GCNNNNNNNGC	9 cut(s)	43, 247, 256, 461, 713, 794, 958, 1508, 1739
NciI	CCSGG	1 cut(s)	23
NdeII	GATC	6 cut(s)	878, 1281, 1318, 1456, 1663, 1675
NlaIII	CATG	6 cut(s)	245, 521, 624, 682, 1217, 1860
NlaIV	GGNNCC	3 cut(s)	174, 1158, 1320
NmeAIII	GCCGAG	1 cut(s)	622
NmuCI	GTSAC	5 cut(s)	85, 93, 616, 1335, 1679
NsiI	ATGCAT	1 cut(s)	1215
NspI	RCATGY	1 cut(s)	1860
NspV	TTCGAA	1 cut(s)	965
PaeR7I	CTCGAG	1 cut(s)	254
PagI	TCATGA	1 cut(s)	517
PceI	AGGCCT	1 cut(s)	46
PciSI	GCTCTTC	1 cut(s)	714
PctI	GAATGC	1 cut(s)	492
PdmI	GAANNNNTTC	2 cut(s)	1070, 1242
PfeI	GAWTC	4 cut(s)	766, 773, 854, 1801
PflMI	CCANNNNNTGG	1 cut(s)	1214
PkrI	GCNGC	8 cut(s)	19, 76, 111, 331, 454, 645, 796, 1513
PleI	GAGTC	4 cut(s)	522, 538, 931, 1105
PpsI	GAGTC	4 cut(s)	522, 538, 931, 1105
Psp124BI	GAGCTC	1 cut(s)	1874
Psp6I	CCWGG	5 cut(s)	89, 973, 1139, 1232, 1429
PspGI	CCWGG	5 cut(s)	89, 973, 1139, 1232, 1429
PspN4I	GGNNCC	3 cut(s)	174, 1158, 1320
PspPI	GGNCC	1 cut(s)	1758
PspXI	VCTCGAGB	1 cut(s)	254
PstI	CTGCAG	2 cut(s)	847, 1516
PsuI	RGATCY	3 cut(s)	878, 1318, 1456
RsaI	GTAC	2 cut(s)	83, 758
RsaNI	GTAC	2 cut(s)	82, 757
RseI	CAYNNNNRTG	3 cut(s)	434, 749, 1766
SacI	GAGCTC	1 cut(s)	1874
SapI	GCTCTTC	1 cut(s)	714
SaqAI	TTAA	5 cut(s)	576, 603, 996, 1700, 1796
SatI	GCNGC	8 cut(s)	18, 75, 110, 330, 453, 644, 795, 1512
Sau3AI	GATC	6 cut(s)	878, 1281, 1318, 1456, 1663, 1675
Sau96I	GGNCC	1 cut(s)	1758
SchI	GAGTC	4 cut(s)	523, 538, 932, 1106
ScrFI	CCNGG	6 cut(s)	23, 91, 975, 1141, 1234, 1431
SduI	GDGCHC	2 cut(s)	1159, 1874
SexAI	ACCWGGT	2 cut(s)	89, 973
SfaNI	GCATC	7 cut(s)	295, 438, 817, 961, 975, 1593, 1884
SfcI	CTRYAG	2 cut(s)	843, 1512
Sfr274I	CTCGAG	1 cut(s)	254
SfuI	TTCGAA	1 cut(s)	965
SinI	GGWCC	1 cut(s)	1758
SlaI	CTCGAG	1 cut(s)	254
SmiMI	CAYNNNNRTG	3 cut(s)	434, 749, 1766
SmlI	CTYRAG	2 cut(s)	254, 858
SmoI	CTYRAG	2 cut(s)	254, 858
SseBI	AGGCCT	1 cut(s)	46
SspMI	CTAG	2 cut(s)	812, 867
SstI	GAGCTC	1 cut(s)	1874
StuI	AGGCCT	1 cut(s)	46
StyD4I	CCNGG	6 cut(s)	21, 89, 973, 1139, 1232, 1429
StyI	CCWWGG	1 cut(s)	1632
TaaI	ACNGT	6 cut(s)	503, 1082, 1135, 1268, 1473, 1902
TaiI	ACGT	2 cut(s)	87, 1766
TaqI	TCGA	6 cut(s)	255, 505, 778, 965, 1607, 1830
TaqII	GACCGA	2 cut(s)	828, 1506
TauI	GCSGC	3 cut(s)	20, 77, 455
TfiI	GAWTC	4 cut(s)	766, 773, 854, 1801
Tru1I	TTAA	5 cut(s)	576, 603, 996, 1700, 1796
Tru9I	TTAA	5 cut(s)	576, 603, 996, 1700, 1796
TscAI	CASTG	2 cut(s)	852, 1138
TseFI	GTSAC	5 cut(s)	85, 93, 616, 1335, 1679
TseI	GCWGC	5 cut(s)	109, 329, 643, 794, 1511
Tsp45I	GTSAC	5 cut(s)	85, 93, 616, 1335, 1679
TspDTI	ATGAA	8 cut(s)	223, 258, 485, 534, 984, 1032, 1079, 1584
TspGWI	ACGGA	1 cut(s)	1797
TspRI	CASTG	2 cut(s)	852, 1138
Van91I	CCANNNNNTGG	1 cut(s)	1214
VpaK11BI	GGWCC	1 cut(s)	1758
XapI	RAATTY	6 cut(s)	395, 695, 832, 1067, 1465, 1907
XceI	RCATGY	1 cut(s)	1860
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1648
XhoI	CTCGAG	1 cut(s)	254
XmnI	GAANNNNTTC	2 cut(s)	1070, 1242
XspI	CTAG	2 cut(s)	812, 867
Zsp2I	ATGCAT	1 cut(s)	1215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.